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Cocoa (Theobroma cacao L.), indigenous to the tropical forests of the Americas, is renowned not only as the primary raw material for chocolate and its derivatives (cocoa liquor and butter) but also as a rich source of phytonutrients with beneficial health effects. Current research has elucidated that within the post-harvest process, fermentation stands as the critical stage for the formation of the principal biochemical quality markers in cocoa, known as polyphenols. These compounds contribute to the bitterness and astringency that constitute the complex flavor profile of chocolate; however, their excessive presence can be organoleptically undesirable. A high phenolic content (>10%) is associated with insufficient fermentation and certain varieties of ordinary cocoa, thereby serving as a discriminatory parameter between fine-flavor cocoa (Nacional) and bulk cocoa (CCN-51). Beyond their technological significance, these components have garnered substantial scientific interest, as polyphenol consumption is associated with potential protective effects against the development of non-communicable chronic diseases (including diabetes, cancer, and atherosclerosis), attributable to their potent antioxidant properties. In this context, the objective of this study was to evaluate the impact of fermentation time on the antioxidant capacity (AC) and total polyphenol content (TPC) in the principal Ecuadorian cocoa varieties (i.e., CCN-51 clone and Nacional). Pilot-scale fermentation experiments demonstrated significant variations in antioxidant capacity (CCN-51 clone: 785.61 to 1852.78 and Nacional: 564.32 to 1428.60 µmol TE/g) and total polyphenol content (CCN-51 clone: 52.92 to 162.82; Nacional: 40.55 to 157.50 mg gallic acid/g). Both parameters decreased markedly throughout the process, with the CCN-51 clone exhibiting greater retention.

This study involved the evaluation of the effect of vacuum pasteurization on physicochemical characteristics (pH, total soluble solids, titratable acidity, chroma, tone, IO, vitamin C, 5-hydroxymethylfurfural), microbiological properties (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, total coliforms, total mesophilic aerobes, molds and yeasts) and sensory characteristics of orange and carrot nectar. The thermal treatments were designed based on the thermal lethality of two heat-resistant microorganisms typical of the product (Neosartorya fischeri and Zygosaccaromyces bailii). The evaluation was carried out on raw nectar and pasteurized nectar. The shelf life was estimated to be 30 days (6 °C). The most favorable results were obtained by applying a heat treatment at 88 °C for 32.68 min, managing to retain 85.87% of vitamin C and a microbiological stability of 12 days (6 ± 0.6 °C) with regard to total mesophilic aerobes. Likewise, the tasters established that this treatment resulted in the best flavor, texture and acceptability characteristics.

Cannabis is an herbaceous species of the Cannabaceae family and is native to the Himalayas. It contains hundreds of metabolites with potential bioactivity, including cannabinoids, terpenes, and flavonoids. The objective of this experiment was to evaluate the yield and the cannabidiol (CBD) and tetrahydrocannabinol (THC) content of medicinal cannabis (variety Cherry Oregon) in two localities of the Ecuadorian highlands. A Randomized Complete Block Design with four treatments and four replications was carried out. Treatment 1 was maintained with natural light, treatment 2 received 16/8 photoperiod (16 hours light, 8 hours dark) for one week, treatment 3 received 16/8 photoperiod for two weeks, and treatment 4 received 16/8 photoperiod for 3 weeks. In the locality 1, treatment 4 showed better values of plant height, dry biomass, biomass yield per plant and greater biomass yield per m2, in both cycles. In the locality 2, the treatments were statistically similar in all variables in both cycles, but a trend where treatment 4 presented higher values compared to the rest of the treatments was observed. The THC and CBD contents for the treatments and cycles ranged between 0.06% and 0.51%, and between 13.76% and 15.29% respectively in both localities. Finally, the results obtained agree with what is reported in the variety's technical sheet, and the THC content does not exceed the maximum value allowed by Ecuadorian regulations.

The physicochemical properties of fruits at different maturity stages using grafting technology are of great importance since grafting can alter the nutritional and functional parameters of the fruit. In this study, grafted yellow pitahaya (Selenicereus megalanthus Haw.) fruit, grown on live tutors, was evaluated from stages 0 to 5. The following response variables were recorded: fruit weight, diameter, and length; pulp weight with seed and peel; color; firmness; total soluble solids content; titratable acidity; pH; total flavonoid content; total polyphenol content; and antioxidant activity determined using FRAP and ABTS. The results show that fruits harvested from grafted plants have better physical characteristics such as fruit weight, diameter, and length. However, the total soluble solids content and titratable acidity were similar in fruits from grafted and ungrafted plants. The highest content of total polyphenols, flavonoids, and antioxidant activity determined by ABTS and FRAP were found in fruits at maturity stage 0, and the content decreased as the fruits ripened. A positive correlation was found between the total polyphenol content, total flavonoid content, and antioxidant capacity with protein content. The S. megalanthus grafting technique is a promising technology for sustainable production because it reduces pesticide use by combatting soil pathogens and not modifying fruit quality.

The phytochemical components and antioxidant capacity of Açaí (Euterpe oleracea) give it nutritional and bioactive characteristics with anti-cancer and anti-inflammatory properties; it is exported mainly from Brazil to various places worldwide. In Ecuador, the cultivated Euterpe oleracea variety has an abundant production that has not been used or studied in depth; because of this, it is relevant to expand the study of this fruit’s phytochemical and antioxidant properties. This paper presents the results of evaluating the concentration of antioxidants and antioxidant activity in different stages of maturation and geographical locations of the Açaí, for which samples obtained in the Ecuadorian provinces of Sucumbíos and Orellana have been evaluated. Antioxidant concentrations were determined with a UV/VIS spectrophotometer at 450–760 nm wavelengths. Antioxidant capacity was determined using the ABTS and FRAP methods. It was evidenced that the values of total polyphenols and total flavonoids decrease with increasing ripening; the opposite effect occurs with total anthocyanins that have a higher concentration in ripe fruits and evidencing an antioxidant capacity that decreases with ripening determined by both methods (FRAP and ABTS).

Quinoa is known for its high nutritional value and adaptability; however, there is a lack of data about the chemical composition of quinoa produced in Ecuador, especially the Chimborazo ecotype. Our research aims to evaluate the nutritional components of Chimborazo quinoa. This knowledge (chemical composition) can help to improve cultivation and farmers’ understanding. Samples were collected from 49 plots at four altitude ranges (3000–3200; 3201–3300; 3301–3400; 3401–3533) m.a.s.l. Each sample of 2 kg quinoa was cleaned, dried (32 °C/15 h), and stored at −20 °C before analyzing water activity, proximate composition, mineral content, antioxidant activity, and functional compounds. The data were analyzed using ANOVA and mean comparison, Pearson correlation, and principal component analysis. The Chimborazo ecotype shows protein content comparable to or exceeding other global quinoa cultivars. Statistical analysis revealed that altitude had a minimal influence on quinoa’s chemical composition, resulting in overlapping altitude-based clusters. Complex relationships between quinoa variables were identified, which varied with altitude. These findings suggest that cultivation of high-quality quinoa across a range of altitudes is feasible without compromising its intrinsic quality. Moreover, the extensive and diverse results from our study provide a solid foundation for further plant breeding and the development of specialized quinoa varieties.

The aim of the study was to determine the profile of bioactive compounds in cocoa residues (mucilage and bean shells), and to evaluate their antioxidant activity in two cocoa varieties, Nacional X Trinitario type (Fine Aroma) and the variety CCN-51. The extraction of phytonutrients from the residues was carried out selectively. The characterization and quantification of the total polyphenol content (TPC), and the total flavonoid content (TFC) were determined by UV–VIS spectrophotometry. High-performance liquid chromatography (HPLC) was used to determine the phenolic profile and methylxanthines. The antioxidant activity was evaluated by the methods of 2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) cation bleaching (ABTS), ferric-reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). The exudate mucilage samples from Nacional X Trinitario-type cocoa presented the highest content of TPC 105.08 mg gallic acid equivalents (GAE)/100 mL, TFC 36.80 mg catechin equivalents (CE)/100 mL, catechin (CAT) 35.44 mg/g, procyanidins (PCB2: 35.10; PCB1: 25.68; PCC1: 16.83 mg/L), epicatechin (EPI) 13.71 mg/L, caffeine (CAF) 0.90% and theobromine (TBR) 2.65%. In the cocoa bean shell, the variety CCN-51 presented a higher content of TPC (42.17 mg GAE/100 g) and TFC (20.57 mg CE/100 g). However, CAT (16.16 mg/g), CAF (0.35%) and TBR (1.28%) were higher in the Nacional X Trinitario cocoa type. The EPI presented no significant differences between the two samples studied (0.83 and 0.84 mg/g). The antioxidant activity values (ABTS, FRAP and ORAC methods) were higher in the samples of CCN-51 than in the Nacional X Trinitario type. The bean shell samples presented antioxidant values of 171.32, 192.22 and 56.87 mg Trolox equivalents (TE)/g, respectively, and the bean shell samples presented antioxidant values of 167.06, 160.06 and 52.53 mg TE/g, respectively. The antioxidant activity (ABTS, FRAP and ORAC) of the residues was correlated with the bioactive compounds of the mucilage and bean shells, showing a strong positive correlation (

Avocado demand has increased in recent years due to the nutraceutical properties that this fruit has and its positive impacts on human health; however, avocado production also requires sustainable alternatives to improve its cultivation. The objective of this study was to carry out characterization of the mineral content and phytochemical compounds in avocado fruit of the Hass variety grown using sustainable agricultural practices in Ecuador. Our results show an increase in fruit quality traits, such as firmness, and in the content of soluble solids, protein, fiber, fat, carotenoids, Ca, Mg, Zn and stearic acid in the pulp of the avocado Hass variety, as well as an initial trend of yield increase with the application of sustainable practices. Moreover, antioxidant activity was associated with polyphenol content. There were positive correlations of Mg with K and Ca, and of flavonoids with linolelaidic, linoleic and linolenic acids. Overall, our results indicate that avocado can be used as a functional and nutritional food due to its phytochemical composition and the mineral content of its pulp, which contributes to the promotion of its consumption and encourages healthy eating. In addition, the use of sustainable practices, such as fertigation and the application of microorganisms, is also promoted for growing avocado.

El orégano (Origanum vulgare L.) es una especie introducida en la flora ecuatoriana. Por sus propiedades es muy apreciada y consumida, por lo que es importante determinar la naturaleza de sus beneficios. En este estudio se evaluó la composición fisicoquímica y el contenido de los compuestos funcionales responsables de la actividad antioxidante de la planta en la accesión ECU-20229 del Banco de Germoplasma del Instituto Nacional de Investigaciones Agropecuarias (INIAP). El estudio se realizó en Italquí provincia de Imbabura y en Tumbaco provincia de Pichincha. Se analizó la planta entera y sus partes (raíz, tallo y hojas), con la finalidad de determinar dónde se encuentra el mayor contenido de proteína, grasa, minerales y los compuestos funcionales. Se realizó el análisis proximal, concentración de proteína, minerales, polifenoles y flavonoides totales, así como la capacidad antioxidante. El contenido de los compuestos analizados fue estadísticamente diferentes entre las partes de la planta analizadas, y entre los sitios estudiados. La concentración de proteína, grasa y carbohidratos fue superior en las hojas que en las otras partes de la planta. La concentración de estos compuestos fue mayor en Tumbaco que en Italquí. Los macro y microminerales se encontraron en mayor cantidad en Italquí, al igual que el contenido de polifenoles y flavonoides totales. La capacidad de antioxidante fue mayor en Tumbaco. En este estudio se han confirmado las propiedades nutritivas descritas para esta planta en beneficio de la salud humana y animal, pero además se aporta información sobre el valor agregado a las partes de la planta que regularmente son desaprovechadas.

There are several species of passion fruit grown in South America. However, there is a lack of information about the mineral content in their pulp. Thus, the objective of the present research was to determine the mineral content in the pulp of different germplasms of passion fruit [Passiflora edulis f. flavicarpa (INIAP 2009 and P10), P. alata (Sweet passion fruit), P. edulis f. edulis (Gulupa) and Passiflora sp. (Criollo POR1 and Criollo PICH1)] grown in Ecuador and to determine their relationship with relevant fruit quality traits. The results showed that high Mg content was associated with less peel thickness, soluble solids was negatively related to K and B content, and vitamin C was negatively related to S content. INIAP 2009 had high titratable acidity and fruit weight but low N and Na; P10 showed the highest contents of N, K, Na, Mn and fruit weight but less P, Mg, and Fe; sweet passion fruit showed high S, Zn, Cu, soluble solids, and peel thickness but low K, Ca, B, and titratable acidity; Gulupa had high Mg, B, and Zn but low S, Fe, and Mn; Criollo POR1 showed high N and Fe but low Zn; and Criollo PICH1 showed high P, Ca, Mg, and Cu but low soluble solids and peel thickness. These results provide additional information on passion fruit germplasm grown in Ecuador and constitutes a reference for further breeding programs.

Tree tomato (Solanum betaceum Cav.) is an Andean fruit crop that is grown in Ecuador. It is an exceptional source of minerals and vitamins, thus has nutraceutical properties. The objective of this research was to carry out a phytochemical characterization of a breeding population composed of 90 segregants. Pulp (including mesocarp, mucilage, seeds and placenta) was ground and sieved in order to obtain the liquid pulp to be lyophilized for the chemical analyzes. Antioxidants compounds were determined by spectrophotometry and vitamin C by reflectometry. Data were analyzed by principal components, grouping, and variance analyses; in addition, Z Score estimation was carried out to select elite individuals. There was a broad variability in the data obtained for the breeding population, polyphenol content varied from 5.11 to 16.59 mg GAE g−1, flavonoids from 1.24 to 6.70 mg cat g−1, carotenoids from 50.39 to 460.72 µg β-carotene g−1, anthocyanins from 1.06 to 240.49 mg cy-3-glu 100 g−1, antioxidant capacity from 49.51 to 312.30 µm Trolox g−1, and vitamin C from 78.29 to 420.16 mg 100 g−1. It can be concluded that tree tomato is a good source of beneficial biocompounds and has a high antioxidant capacity.

Tropical fruits are in high demand for their flavor and for their functional composition because these compounds are considered nutraceuticals. Passion fruit production is of economic importance to Ecuador; however, several Passiflora species are grown and each has to be analyzed to identify their phytochemical composition. In this study, the polyphenol, flavonoid, carotenoid, vitamin C, sugar and organic acid contents were determined. Six different Passiflora spp. germplasms were analyzed, coming from Passiflora edulis f. flavicarpa, Passiflora alata, Passiflora edulis f. edulis and unidentified Passiflora species (local germplasm). Measurement techniques included reflectometry for vitamin C, spectrophotometry for antioxidant compounds and HPLC for sugars and organic acids. Data were analyzed by principal component analysis, correlation and analysis of variance. Results showed that INIAP 2009 and P10 showed a high amount of polyphenols, antioxidant activity and citric content. Sweet passion fruit had the lowest vitamin C content while Gulupa showed the highest content. In terms of the local germplasm, POR1 showed the lowest content of flavonoids while PICH1 had high flavonoid and carotenoid content. Polyphenols were the main compounds that influenced antioxidant activity. This phytochemical information adds value to passion fruit as a nutraceutical source.

Andean blackberries (Rubus glaucus Benth) are fruits rich in phytocomponents with high antioxidant activity. In this work, the changes in the total polyphenol content (TPC), the total flavonoid content (TFC), and the total anthocyanin content (TAC) of four blackberry varieties at three maturity stages (E1-25%, E2-50%, and E3-100%) were measured. The antioxidant activity (AA) was evaluated using the 2,2’azinobis-(3-ethylbenzthiazolin 6-sulphonic acid (ABTS) and ferric reducing antioxidant power (FRAP) methods. TPC and TFC content decreased with the increase in the maturity stage. The blackberry Brazos cultivar presented TPC values of 51.26, 38.16, and 31.59 mg of gallic acid equivalents (GAE)/g dry weight (DW) at E1, E2, and E3, respectively. The TAC and soluble solids increased with the increase in the maturity stage of the fruits. The Andimora variety at E3 presented a high TPC content, and the Colombiana variety presented a high TFC content. The blackberry Colombiana cultivar presented TAC values of 1.40, 2.95, and 12.26 mg cy-3-glu/100g DW at E1, E2, and E3, respectively. The blackberry Colombiana cultivar presented a high AA value at 1278.63 µmol TE/g DW according to the ABTS method and 1284.55 µmol TE/g DW according to the FRAP method. The TPC and TFC showed a high correlation with the AA according to the ABTS and the FRAP methods. The Pearson correlation between the TFC and AA/ABTS has a value of r = 0.92. The TFC and AA/FRAP present a value of r = 0.94.

Ecuador Andean purple corn (Zea mays L.) was subjected to a germination process at 15–40 °C for 24–168 incubation hours. Purple corn protein concentrates (PCPCs) were obtained by alkaline extraction at pH 8.0 and pH 10.0, followed by an isoelectric precipitation process at pH 4.0, pH 5.0 and pH 6.0. Proteins and phenolic content of PCPCs was calculated. PCPC antioxidant properties were determined by the ferric-reducing antioxidant power (FRAP) in vitro method and by the 2,2-azinobis, 3-ethyl-benzothiazoline-6-sulfonic acid, (ABTS) in vitro method. Andean purple corn seeds were able to germinate under the germination conditions tested in this study. The higher percentage of germination was of 63.33% at 168 h/25 °C. The PCPCs protein profile was characterized for the presence of six bands with molecular weights of 14.50 kDa, 20.12 kDa, 25.18 kDa, 41.85 kDa, 59.59 kDa, and 65.87 kDa. Germinated PCPC presented a high TPC content with ranges of 605.71–1820.00 mg gallic acid equivalents (GAE)/g PCPC dry weight (DW), germinated PCPC/72 h/25 °C presented a higher value of 1820.00 mg GAE/g PCPC, DW. All germinated PCPCs samples assayed presented strong antioxidant activity when measured by the ABTS and FRAP methods. Germinated PCPC/144 h/35 °C presented high antioxidant activity by ABTS with 804.35 µmol Trolox equivalents (TE)/g PCPC DW and germinated PCPC/144 h/30 °C presented a high value by the FRAP method, 987.83 µmol TE/g PCPC DW.

Crop productivity and food quality are affected by environmental conditions. The objective of this work was to evaluate the effect of the environment on the concentration of phytochemical components in several potato (Solanum tuberosum) cultivars. The content of vitamin C (ascorbic acid, AA), the total carotenoids content (TCC), the total polyphenols content (TPC), and the total anthocyanins content (TAC) of 11 potatoes varieties grown in Ecuador (Cutuglahua, Pujilí, and Pilahuín) was measured by the spectrophotometric method. The antioxidant capacity (AC) of potato cultivars was evaluated by the ABTS method. The AA concentration ranged between 12.67 to 39.49 mg/100g fresh weight (FW), the TCC ranged between 50.00 and 1043.50 μg/100g FW, the TPC ranged between 0.41 and 3.25 g of gallic acid equivalents (GAE)/kg dry weight (DW), the TAC ranged between 2.74 and 172.53 μg/g FW and finally the AC ranged between 36.80 and 789.19 μg of trolox equivalents (TE)/g FW. Genotypes (G), location (L), and interaction (G x L) were significant at p < 0.01. The genotype (G) showed a greater variation in the phytochemical contents. AA and TPC showed the highest correlation with the AC. A selection of genotypes with these characteristics can be used to develop germplasms with a high AC.

Anthocyanins, carotenoids and polyphenols are biomolecules that give the characteristic color to fruits. Carotenoids relate to yellow, orange and red colors whereas anthocyanins and polyphenols mainly relate to purple and red colors. Presently, standard determination of antioxidants is carried out using relatively complex methods and techniques. The aim of this study was to develop a mathematical prediction model to relate the internal color parameters of the Amazonic fruits araza (Eugenia stipitata Mc Vaugh), Andean fruit blackberry (Rubus glaucus Benth), Andean blueberry (Vaccinium floribundum Kunth), goldenberry (Physalis peruviana L.), naranjilla (Solanum quitoense Lam.), and tamarillo (Solanum betaceum Cav.) to their respective anthocyanins, carotenoids and polyphenols contents. The mathematical model was effective in predicting the total anthocyanins content (TAC), the total carotenoids content (TCC) and finally the total phenolic content (TPC) of fruits assayed. Andean blueberry presented a TPC with an experimental value of 7254.62 (mg GAE/100 g sample) with respect to a TPC prediction value of 7315.73 (mg GAE/100 g sample). Andean blackberry presented a TAC with an experimental value of 1416.69 (mg chloride cyanidin 3-glucoside/100 g) with respect to a prediction TAC value of 1413 (mg chloride cyanidin 3-glucoside/100 g).