1. Anaplasma phagocytophilum from Rodents and Sheep, China
- Author
-
Lin Zhan, Wu-Chun Cao, Jia-Fu Jiang, Xiao-Ai Zhang, Yun-Xi Liu, Xiao-Ming Wu, Wen-Yi Zhang, Pan-He Zhang, Chang-Ling Bian, J. Stephen Dumler, Hong Yang, Shu-Qing Zuo, Chen-Yi Chu, Li-Qun Fang, Jan H. Richardus, and J. Dik F. Habbema
- Subjects
Anaplasma phagocytophilum ,isolation ,rodents ,sheep ,China ,rickettsia ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
- Published
- 2010
- Full Text
- View/download PDF