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1. European Handbook of Plant Diseases

Author

I. M. Smith, J. Dunez, D. H. Phillips, R. A. Lelliott, S. A. Archer, I. M. Smith, J. Dunez, D. H. Phillips, R. A. Lelliott, S. A. Archer

Published
2009

2. Abstracts of presentations on plant protection issues at the xth international congress of virology: August 11-16, 1996 Binyanei haOoma, Jerusalem Iarael part 3(final part)

Author

O. Faktor, G. P. Lomonossoff, S. Souche, B. Levine, C. Ford, D. Schumann, Sh. Abo-El-Saad, Fuh-Jyh Jan, F. Revere, J. Zalewski, M. Ravelonandro, M. Zaitlin, K. Lento, V. Hardi, V. Gaba, A. Romero, M. A. El-Saied, Michael J. Adams, O. N. Denisenko, M. Pilowsky, B. Böddi, W. Zagórski, M. Kikkert, J. Nahring, J. Peterka, U. Oertel, S. Seal, Kristin D. Kasschau, Susan Phillips, David C. Baulcombe, Michael G. K. Jones, S. A. MacFarlane, David M. Tricoli, Phil Larkin, M. A. Mayo, Maja Ravnikar, Patricio Arce-Johnson, H. Uchino, P. Bodegom, T. J. Higgins, Aiming Wang, Roger N. Beachy, T. Lin, M. I. Boulton, T. Natsuaki, S. Astier, A. de Kochko, E. Meiri, Marc G. Fortin, Orit Edelbaum, K. H. Hellwald, B. Falk, M. Ikegami, R. S. Zemetra, D. E. Purcifull, Rafael Rivera-Bustamante, X. W. Xiao, W. K. Kaniewski, M. M. Maugeri, Silvy Zeituny, N. H. Soliman, A. A. Brunt, S. Zimmermann, Hector D. Quemada, J. A. Daròs, I. Sela, I. Oukropec, Ab van Kammen, E. Dagless, R. Carson, P. de Haan, D. D. Shukla, P. J. Shiel, T. Ruiz-Perez, Darja Barlič Maganja, S. C. Taylor, Kristina Gruden, G. Salem, Varsha Wesley, D. L. Gellatly, V. Thole, H. Kawashima, E. Pehu, Erwin Cardol, P. Kominek, L. Mehlo, J. van Lent, K. Graichen, Frank Rabenstein, D. Robinson, J. Walter, M. Kelve, C. Porta, L. Tabe, M. El-Hariry, Johannes Fütterer, Stephen J. Wylie, A. Merits, Roger Hull, R. Goldbach, R. Arroyo, D. K. Becker, A. Ragozzino, H. Chatel, Velitchka Nikolaeva, J. F. Bol, N. Paltridge, G. I. Atiri, K. M. Taylor, A. N. Chiang, Andrew Wieczorek, K. H. Ryu, J. Polák, Y. Chupeau, J. C. Thouvenel, Daniel Plante, Na-Sheng Lin, J. Angel-Diaz, J. Porter, D. Gahakwa, A. Mooney, M. J. Frenkel, S. D. Wyatt, P. Chu, Brandt G. Cassidy, A. Van Schepen, G. R. Smith, A. R. De Rover, L. Sela, Y. Stram, Margarita Yankulova, T. Otsubo, O. Livneh, S. Tsuda, M. R. Hajimorad, J. E. Johnson, G. D. May, L. Levy, T. Candresse, Yan Yitang, Marilyn J. Roossinck, D. J. Hwang, C. M. Fauquet, J. J. L. Gielen, S. Lock, David Charles Baulcombe, B. J. Shi, M. Bitoova, Duk-Ju Hwang, E. Ham, V. M. Ursin, M. Barba, B. L. Barrón, Y. Kageyama, P. Palukaitis, Yau-Heiu Hsu, E. Aliskevicius, O. Le Gall, J. Antoniw, C. J. Arntzen, J. A. Sanchez-Navarro, T. Tamm, W. Chaleeprom, S. Dinant, S. Yu. Morozov, Biing-Yuan Lin, J. L. Badge, S. S. Bishnoi, J. L. Dale, R. Kormelink, N. E. Turner, J. P. T. Valkonen, A. E. Aboul-Ata, S. W. Ding, C. W. A. Pleij, Chu-Hui Chiang, Thomas Hohn, S. Kashiwazaki, F. Ponz, A. M. Chachulska, A. Almási, E. Vardi, J. M. Martínez-Zapater, E. Hiebert, P. Roy, Sheldon Cohen, J. Kunhara, M. Saarma, E. Truve, M. Taliansky, Marcel Prins, Joze Brzin, R. H. Symons, M. Leech, Sheng-Zhi Pang, D. A. Zelenina, M. Storms, M. Saito, M. Kinta, K. Tomaru, Peter de Haan, F. E. Christensen, J. Rathjen, S. J. Yang, E. Fuchs, A. Zelcer, I. B. Kaplan, E. C. Lawson, Hélène Sanfaçon, B. C. Rodoni, G. Mazithulela, Ching-Hsien Wang, M. Ekes, S. Wittmann, S. Schillberg, T. Maki-Valkama, E. Sivamani, M. Shams-Bakhsh, R. Turner, E. Mueller, Borut Štrukelj, M. Karamagioli, V. Somsap, A. Hernádez, A. Golshani, H. P. Loo, E. Ramirez, P. W. G. Chu, Colin W. Ward, T. B. Clifford, N. Collins, K. M. Makkouk, Amis Druka, G. D. Foster, T. Mitsky, M. F. Bateson, Igor Kregar, A. Mathews, Maria-Rosa Marano, L. Rasochova, James C. Carrington, C. A. Powell, K. Makinen, P. H. Berger, Mounir G. AbouHaidar, Nilgun Turner, J. J. English, F. Blaise, M. Q. J. M. Van Grinsven, Helen Rothnie, Frank Ratcliff, Rahat Makhdoom, Kim Carney, Y. M. Hou, R. L. Gilbertson, I. García-Luque, M. T. Serra, G. I. Dwyer, A. Murayama, Rob W. Goldbach, F. DiSerio, C. Jacquet, N. Banerjee, Stanislaw Flasinski, D. Twell, C. Kusiak, M. Chrzanowska, J. Feldman, Lidia Ivanova, Karma Carrier, John F. Bol, W. A. Miller, B. Raccah, J. A. Handley, J. Bachelier, T. Tamada, L. Nigul, R. N. Beachy, T. Kuittinen, R. Floras, A. K. Koenen, S. Guelemerov, Dennis Gonsalves, Joan Wellink, P. Gilardi, Monique M. van Oers, T. Kiguchi, L. Chen, Moshe Lapidot, G. Adam, D. H. L. Bishop, Ivan Ivanov, Shyi-Dong Yeh, G. Rodriguez-Alvarado, A. Callahan, G. Davenport, R. Stratford, B. Passmore, J. E. Faure, R. Gaborianvi, Gang Chen, Richard Kormelink, H. Huet, P. L. McCarthy, J. Drossard, Titia Sijen, R. Fischer, Z. Szigeti, P. Christou, D. Wolf, J. F. Laliberté, J. G. Atabekov, L. López, L. Tomassoli, B. A. L. M. Deiman, L. Kuznetsova, M. A. Wurscher, C. Robaglia, Q. Li, R. B. Sangwan, J. Dunez, R. M. Harding, Amit Gal-On, V. E. Spall, M. J. Soto, J. Hansen, N. Sugimura, C. A. Ong, G. P. Lodhi, Huub J. M. Linthorst, D. Li, R. Scorza, W. X. Li, Narayan Rishi, J. Schubert, M. Aulik, H. Lot, T. Kusume, R. Sanders, J. W. Davies, M. Satour, O. N. Fedorkin, G. A. Prody, M. V. Mikhailov, G. J. Hafner, Sandra Corsten, T. Pehu, Claire Huguenot, F. Garcia-Arenal, Y. P. Duan, D. Marshall, V. Pallas, A. Atanassov, P. Sithisarn-Burns, J. C. Bachelier, and J. Albouy

Subjects
0106 biological sciences, 010602 entomology, Insect Science, Ecology (disciplines), International congress, Physiology, Library science, Plant Science, Biology, 01 natural sciences, 010606 plant biology & botany
Published
2018

4. Abstracts of presentations on plant protection issues at the fifth international Mango Symposium Abstracts of presentations on plant protection issues at the Xth international congress of Virology

Author

J. E. Peña, M. Wysoki, Gajendra Singh, Nancy Boscán de M., Freddy J. Godoy, A. Obligado, C. J. Rossetto, I. J. A. Ribeiro, P. B. Gallo, N. B. Soares, J. C. Sabino, A. L. M. Martins, N. Bortoletto, R. C. Ploetz, D. Benscher, Aimé Vázquez, A. Colls, Julianne Nagel, B. Schaffer, Y. Pinkas, M. Maymon, S. Freeman, Mikhail Bostros Bastawros, M. J. Gosbee, G. I. Johnson, D. C. Joyce, J. A. G. Irwin, W. C. Saaiman, D. Prusky, E. Falik, I. Kobiler, Y. Fuchs, G. Zauberman, E. Pesis, M. Ackerman, I. Roth, A. Weksler, O. Yekutiely, A. Waisblum, A. Keinan, G. Ofek, R. Reved, R. Barak, P. Bel, L. Artes, N. Visarathanonth, Z. Xu, L. Ponce de León, C. Muñoz, L. Pérez, F. Diaz de León, C. Kerbel, S. Esparza, E. Bósquez, M. Trinidad, L. M. Coates, A. W. Cooke, J. R. Dean, Ana Lucia Duarte, Paulo Alberto Otto, Aldo Malavasi, M. C. C. Lizado, M. L. Bautista, L. A. Artes, N. S. Bacalangco, U. Farungsang, N. Farungsang, D. P. Waskar, S. D. Masalkar, R. S. Gaikwad, S. V. Damame, Ian S. E. Bally, Tim J. O’Hare, Rowland J. Holmes, J. G. Atabekov, Claude M. Fauquet, O. Tomori, D. L. Nuss, P. Ahlquist, J. Díez, M. Ishikawa, M. Janda, B. D. Price, M. Restrepo-Hartwig, J. F. Bol, C. M. A. van Rossum, M. L. Garcia, E. A. G. van der Vossen, Chantal B. E. M. Reusken, T. R. Canto, A. Gal-On, P. Palukaitis, M. J. Roossinck, S. Flasinski, Maria A. Restrepo-Hartwig, Paul Ahlquist, Ekaterina Smirnyagina, Na-Sheng Lin, Peter D. Nagy, Marek Figlerowicz, Jozef J. Bujarski, D. F. Proll, K. J. Guyatt, A. D. Davidson, Kook-Hyung Kim, Eric Miller, Cynthia Hemenway, Z. Havelda, T. Dalmay, J. Burgyán, C. M. Kearney, M. Thomson, K. E. Roland, W. O. Dawson, Y. Bao, S. A. Carter, R. S. Nelson, P. M. Derrick, Xin Shun Ding, J. K. Eskarous, S. Sarkar, M. El-Shamy, J. Chen, N. Sako, W. Yuichiro, K. Ohshima, Y. Okada, Brice Felden, Yuri G. Kuznetsov, Alexander J. Malkin, Aaron Greenwood, Alexander McPherson, K. I. Ivanov, Y. L. Dorokhov, C. H. Kim, Katalin Sálanki, Isabelle Carrére, Mireille Jacquemond, Mark Tepfer, Ervin Balazs, A. I. Sanz, M. T. Serra, I. García-Luque, F. Revers, T. Candresse, O. LeGall, S. Souche, H. Lot, J. Dunez, E. Cecchini, J. Milner, N. Al-Kaff, S. Covey, Z. Gong, C. Geri, S. N. Covey, K. R. Richert-Pöggeler, R. J. Shepherd, R. Casper, Eti Meiri, B. Raccah, A. Gera, S. Singer, E. K. Allam, Soheir I. El Afifi, M. A. Abo El Nasr, M. H. Abd El Ghaffar, I. Elisabeth Johansen, K. E. Keller, R. O. Hampton, Karina SÕrensen, S. S. Bishnoi, Narayan Rishi, M. Y. D. Gumedzoe, K. Atissime, S. Yedibahoma, Joan Wellink, Jan Verver, Peter Bertens, Jan van Lent, Rob W. Goldbach, Ab van Kammen, Annemarie Lekkerkerker, K. M. Taylor, V. E. Spall, G. P. Lomonossoff, S. Yu. Morozov, A. G. Solovyev, D. A. Zelenina, E. I. Savenkov, V. Z. Grdzelishvili, S. Y. Morozov, K. A. J. Jansen, C. J. A. M. Wolfs, H. Lohuis, B. J. M. Verduin, V. A. Stein-Margolina, Y. H. Hsu, B. Y. Chang, N. S. Lin, Marcel Pilartz, Holger Jeske, Jeanmarie Verchot, David C. Baulcombe, David J. English, E. Müller, D. C. Baulcombe, Isabelle Malcuit, Tony Kavanagh, J. P. T. Valkonen, Ü. Puurand, A. Merits, F. Rabinstein, O. Sorri, M. Saarma, Y. C. Liao, C. Vaquero-Martin, M. Monecke, W. Rohde, D. Prüfer, R. Fischer, Y. Antignus, O. Lachman, M. Pearlsman, S. Cohen, W. P. Qiu, J. W. Moyer, A. Feldhoff, M. Kikkert, R. Kormelink, G. Krczal, D. Peters, György Szittya, József Burgyán, K. Wvpijewski, E. Paduch-Cichal, A. Rezler, S. Skrzeczkowska, J. Augustyniak, L. Nemchinov, E. Maiss, A. Hadidi, Anita Wittner, László Palkovics, Ervin Balázs, A. Crescenzi, P. Piazzolla, A. Kheyr-Pour, G. A. Dafalla, H. Lecoq, B. Gronenborn, U. Bauer, I. Laux, M. R. Hajimorad, X. S. Ding, Stanislaw Flasinski, Pour G. Cassidy, B. Dugdale, P. R. Beetham, R. M. Harding, J. L. Dale, G. Qiu, J. G. Shaw, A. Molnár, P. Más, J. M. Balsalobre, M. A. Sánchez-Pina, V. Pallás, J. Rahontei, L. López, J. J. Lázara, M. Barón, R. A. Owens, G. Steger, Y. Hu, A. Fels, R. W. Hammond, D. Riesner, A. R. W. Schröder, A. Góra, J. Pawlowicz, A. Kierzek, W. Zagorski, T. Baumstark, W. Schiebel, R. Schiebel, A. Axmann, B. Haas, H. L. Sänger, Yang Xicai, Yie Yin, Zhu Feng, Liu Yule, Kang Liangyi, Tien Po, H. Poliyka, U. Staub, M. Wagner, H. J. Gross, Teruo Sano, Akiro Ishiguro, J. Fayos, R. Garro, J. M. Bellés, V. Conejero, R. G. Bonfiglioli, D. R. Webb, R. H. Symons, K. A. El-Dougdoug, A. A. Abo-Zeid, S. Ambrós, C. Hernandez, J. C. C. Desvignes, R. Flores, M. d’Aquilio, V. Lisa, G. Boccardo, A. Vera, J. A. Daròs, J. Henkel, R. Spieker, C. Higgins, R. Turley, D. Chamberlain, M. Bateson, J. Dale, L. d’Aquino, A. Ragozzino, J. Henderson, M. F. Bateson, W. Chaleeprom, A. J. Gibbs, K. Graichen, F. Rabenstein, E. Schliephake, H. G. Smith, M. Stevens, E. Sadowy, D. Hulanicka, B. Wegener, M. T. Martin, T. Wetzel, G. Cook, G. G. F. Kasdorf, G. Pietersen, Kathryn S. Braithwaite, Cherie F. Gambley, Grant R. Smith, Arnis Druka, Lucille Villegas, Ganesh Dahal, Roger Hull, N. A. Senchugova, C. Büchen-Osmond, M. J. Dallwitz, L. D. Blaine, P. S. Naik, A. B. Sonone, A. S. Kolaskar, J. Y. Sgro, A. C. Palmenberg, Denis Leclerc, Thomas Hohn, E. Moriones, A. Batlle, M. Luis, J. Alvarez, J. J. Bernal, J. L. Alonso, J. Spak, D. Kubelkova, T. T. Kuo, K. K. Gachechiladze, R. S. Adamia, N. S. Balardshishvili, T. G. Chanishvili, D. H. Krüger, Tibor Nagy, Péter Élö, Péter Papp, László Orosz, N. Licis, V. Berzins, Carlos A. Sariol-Carbelo, C. M. RodrCarlos, D. Janzen, Colin W. Ward, S. W. Scott, P. J. Shiel, P. H. Berger, M. E. Aleman, R. N. Beachy, C. M. Fauquet, S. N. Salm, E. P. Rybicki, M. E. C. Rey, R. W. Briddon, G. Harper, A. Druka, S. Phillips, A. A. Brunt, R. Hull, Jo Hay, Indranil Dasgupta, Fan Zaifeng, Brian M. Meehan, Daniel Todd, Hans-Jörk Bunk, F. Grieco, G. P. Martelli, P. Saldarelli, A. Minafra, A. Morag, M. Mumcuoglu, T. Baybikov, M. Schlesinger, Z. Zakay-Rones, B. Shohat, M. Shohat, M. Miller, M. Shaklay, Z. Kalvatchev, R. Walder, D. Garzaro, M. Barrios, Ali Karagöz, Avni Kuru, M. R. Karim, A. J. Johnson, S. Takida, M. C. Thompson, H. M. K. Omer, O. L. M. Omer, L. Biyiti, R. H. Amvam, G. Lamaty, P. Bouchet, J. Xu, K. L. Hefferon, M. G. Abou Haidar, and A. X. X. Meng

Subjects
0106 biological sciences, Zucchini yellow mosaic virus, Barley stripe mosaic virus, biology, Ecology (disciplines), Plant Science, Coat protein, biology.organism_classification, 01 natural sciences, Cucumber mosaic virus, 010602 entomology, Insect Science, International congress, Botany, 010606 plant biology & botany
Published
1997
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6. Immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants

Author

M. Ravelonandro, Olivier Peyruchaud, G. de Marcillac, J. Dunez, L. Garrigue, Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), and Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Transcription, Genetic, viruses, Nicotiana tabacum, Blotting, Western, Genetic Vectors, Molecular Sequence Data, In Vitro Techniques, 01 natural sciences, Virus, Plant Viruses, 03 medical and health sciences, Plasmid, Cistron, Virology, Endopeptidases, Tobacco, Protein biosynthesis, Animals, Amino Acid Sequence, Gene, Plant Diseases, 030304 developmental biology, 0303 health sciences, Base Sequence, biology, fungi, Potyvirus, food and beverages, General Medicine, Agrobacterium tumefaciens, Plants, Genetically Modified, PLUM POX VIRUS PHYTOPATHOGENE, biology.organism_classification, Precipitin Tests, Molecular biology, VIROLOGIE, Plants, Toxic, PLANTE TRANSGENETIQUE, Protein Biosynthesis, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Rabbits, Protein Processing, Post-Translational, Plasmids, 010606 plant biology & botany
Abstract

International audience; Tobacco plants (Nicotiana tabacum cv. Xanthi) have been transformed viaAgrobacterium tumefaciens vectors, with cDNAs corresponding to the plum pox virus (PPV) cistron 2 encoding helper component (HC-Pro) and with the first two and half cistrons of the PPV genome. Presence of the HC-Pro in PPV-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the PPV HC-Pro. The results suggest that two proteases are involved in the processing of the PPV N-terminal polyprotein to yield a protein of 48 k (HC-Pro). HC-Pro autolytically cleaves at its carboxyl-terminus and a proteolytic activity, probably associated with the protein (P 1) encoded by the cistron 1, is required for the cleavage in planta between the proteins derived from cistrons 1 and 2.

Published
1993
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20. Ascomycetes IV: Dothideales

Author

D. H. Phillips, I. M. Smith, R. A. Lelliott, S. A. Archer, and J. Dunez

Subjects
Dothideales, biology, Botany, biology.organism_classification
Published
2009
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22. Isolation and partial characterization of a new virus infecting Prunus armeniaca (apricot) and belonging to the Nepovirus genus

Author

P. Gentit, R.P. Delbos, J. Dunez, T. Candresse, ProdInra, Migration, Génomique, développement et pouvoir pathogène (GD2P), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, biology, [SDV]Life Sciences [q-bio], 04 agricultural and veterinary sciences, Horticulture, biology.organism_classification, Isolation (microbiology), 01 natural sciences, Prunus armeniaca, Virology, Virus, VIROLOGIE, [SDV] Life Sciences [q-bio], Genus, Botany, 040103 agronomy & agriculture, Nepovirus, 0401 agriculture, forestry, and fisheries, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany
Abstract

International audience

Published
2001

23. High resistance and control of biological risks in transgenic plants expressing modified plum pox virus coat protein

Author

C, Jacquet, M, Ravelonandro, and J, Dunez

Subjects
Plants, Toxic, Capsid, Transformation, Genetic, Plum Pox Virus, Virulence, Mosaic Viruses, Aphids, Tobacco, Animals, Transgenes, Plants, Genetically Modified, Risk Assessment, Plant Diseases
Abstract

Transgenic plums transformed with the plum pox virus coat protein (PPV CP) gene displayed a resistance to the sharka disease (Ravelonandro et al., 1997). However, the expression of PPV CP in transgenic plants may lead to complementation of deficient characteristic of an incoming potyvirus. Indeed, an aphid-intransmissible strain of zucchini yellow mosaic virus (ZYMV-NAT) could be transmitted when encapsidated by the engineered PPV CP (Lecoq et al., 1993). To control such a risk, new PPV CP constructs were designed and introduced into Nicotiana benthamiana genome. In the first construct, the DAG amino acid triplet involved in the potyvirus aphid-transmission was deleted. The second construct encoded a truncated PPV CP lacking its first 140 amino acids. In the last construct, the nucleotides encoding the charged amino-acids R220, Q221 and D264 localized in the core of the PPV CP were removed. A bacterial expression system was developed to show that these deletions prevent the assembly of the PPV CP subunits. For each construct, several transgenic lines were produced and first challenged with several strains of PPV. Two phenotypes of resistance were observed: recovery and immunity. Their biochemical characterization showed that the resistance was RNA-mediated and therefore can be classified as homology-dependent (Jacquet et al., 1998a). Resistant lines producing high level of wild type or modified PPV CP were then inoculated with ZYMV-NAT to perform an aphid-transmission assay. Results of these experiments demonstrated that the use of modified forms of PPV CP genes in transgenic plants provide a good way to control the biological risks associated with heteroencapsidation (Jacquet et al., 1998b).

Published
1999

24. Characterization of phenotype resistance to plum pox of transgenic plums expressing plum pox virus capsid gene

Author

M, Ravelonandro, R, Scorza, and J, Dunez

Subjects
Capsid, Plum Pox Virus, Virulence, Reverse Transcriptase Polymerase Chain Reaction, Fruit, Enzyme-Linked Immunosorbent Assay, Rosales, Plants, Genetically Modified, Plant Diseases, Trees
Abstract

Resistance to plum pox virus (PPV) infection can be obtained in transgenic plants that express the virus capsid gene. An Agrobacterium-mediated transformation was used to introduce the PPV capsid gene into Prunus domestica plants. Over 11 regenerated plants (clones) were observed for the development of the disease symptoms and analysed for the presence of PPV by enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and reverse transcription-polymerase chain reaction (RT-PCR) through 4 dormancy cycles. The level of protection against PPV was determined in the transformed plants, non-transformed plants, and a control transgenic plant "transformed" with the plasmid vector alone. One clone, C-5, appeared fully protected, while PT-6 and C-4 clones accumulated a low concentration of virus and the rest of the clones was entirely susceptible. Little is known about the mechanisms of resistance to virus infection in transgenic woody plants. To investigate this aspect, comparative studies based on the characteristics of resistant and susceptible clones have been started. A question, whether the phenotype resistance of clone C-5 is similar to that observed in transgenic herbaceous plants or not, has been addressed. Recent progress in this investigation is presented.

Published
1999

25. Analysis of plum pox virus variability and development of strain-specific PCR assays

Author

J. Dunez, T. Candresse, G. Macquaire, L. Quiot-Douine, Jean-Bernard Quiot, M. Lanne, M. Bousalem, Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and ProdInra, Migration

Subjects
0106 biological sciences, 0303 health sciences, Strain (chemistry), [SDV]Life Sciences [q-bio], Pcr assay, Horticulture, Biology, 01 natural sciences, Virology, VIROLOGIE, [SDV] Life Sciences [q-bio], 03 medical and health sciences, Pox virus, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience

Published
1994

27. In vitro and in vivo expression of PPV helper-component gene

Author

Olivier Peyruchaud, L. Garrigue, J. Dunez, Geneviève Tavert-Roudet, M. Ravelonandro, Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), and ProdInra, Migration

Subjects
0106 biological sciences, Chemistry, [SDV]Life Sciences [q-bio], VECTEUR, 04 agricultural and veterinary sciences, Horticulture, 01 natural sciences, In vitro, Cell biology, VIROLOGIE, [SDV] Life Sciences [q-bio], FACTEUR INSISTANT, In vivo, Component (UML), 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Gene, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany
Abstract

International audience

Published
1991

28. Molecular techniques for plum pox potyvirus (PPV) detection and cloning of the genomic RNA from an atypical strain : PPV EL AMAR

Author

T. Candresse, T. Wetzel, G. Macquaire, R.P. Delbos, A.E. Aboul Ata, H. M. Mazyad, M. Ravelonandro, J. Dunez, Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), and ProdInra, Migration

Subjects
0106 biological sciences, Cloning, Strain (biology), [SDV]Life Sciences [q-bio], AMPLIFICATION CHAINE POLYMERASE, Horticulture, Biology, BIOLOGIE MOLECULAIRE, 01 natural sciences, Virology, 3. Good health, Plum pox potyvirus, [SDV] Life Sciences [q-bio], 03 medical and health sciences, 0302 clinical medicine, Genomic rna, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany, 030215 immunology
Abstract

International audience

Published
1991

29. Transgenic tobacco plants that contain the plum pox virus (PPV) coat protein gene

Author

J. Dunez, M. Ravelonandro, M. Monsion, R.P. Delbos, Pierre-Yves Teycheney, Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), and ProdInra, Migration

Subjects
0106 biological sciences, 0303 health sciences, Transgene, [SDV]Life Sciences [q-bio], Horticulture, Biology, Coat protein, 01 natural sciences, Virology, Plum pox virus PPV, VIROLOGIE, [SDV] Life Sciences [q-bio], 03 medical and health sciences, Gene, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience

Published
1991

30. Nucleotide sequence of the 3'-terminal region of the RNA of the El Amar strain of plum pox potyvirus

Author

T, Wetzel, T, Candresse, M, Ravelonandro, R P, Delbos, H, Mazyad, A E, Aboul-Ata, and J, Dunez

Subjects
Base Sequence, Molecular Sequence Data, Restriction Mapping, DNA-Directed RNA Polymerases, Plant Viruses, Open Reading Frames, Viral Proteins, Capsid, Microcomputers, Endopeptidases, RNA, Viral, Amino Acid Sequence, Cloning, Molecular, Software
Abstract

The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putative 6K protein, the NIa protease, the NIb RNA polymerase and the coat protein genes, linked together as one large open reading frame (ORF) in a fashion similar to the canonical genomic organization of other potyviruses. The large ORF encoding the polyprotein is followed by a 217 nucleotide non-coding region and a poly(A) tail. However, whereas the three PPV strains previously sequenced show levels of identity in excess of 98%, PPV-El Amar shows levels of heterogeneity of 20% in the nucleotide sequence and 10% in the amino acid sequence, when compared with these previously sequenced strains. The N-terminal region of the capsid protein, postulated to be involved in the aphid transmission mechanism of the virus, was found to be the region which differed most between PPV-El Amar and the other strains.

Published
1991

31. Genomic organization of plum pox virus and recombinant DNA technology to combat plum pox virus

Author

J. Dunez, T. Wetzel, Pierre-Yves Teycheney, M. Monsion, G. Tavert, René Delbos, M. Ravelonandro, ProdInra, Migration, Unité mixte de recherche santé végétale, and Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)

Subjects
0106 biological sciences, medicine.medical_specialty, [SDV]Life Sciences [q-bio], Horticulture, Biology, 01 natural sciences, law.invention, 03 medical and health sciences, law, Molecular genetics, Plant virus, PRUNIER, medicine, Pox virus, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Genomic organization, Genetics, 0303 health sciences, Virology, PHYTOPATOLOGIE, 3. Good health, [SDV] Life Sciences [q-bio], INSECTE, LUTTE, Recombinant DNA, ADN RECOMBINANT, 010606 plant biology & botany
Abstract

International audience

Published
1990

33. Abstracts of papers presented at a Symposium on Biological Control and Microbial Interactions Abstracts of papers presented at the French-Israeli Colloquium on Selection of Plants for Disease Resistance Abstracts of papers presented at the 3rd Meeting on Pheromone Research in Israel

Author

G. Loebenstein, J. Halperin, B. Doz, J. B. Marder, Tamar Kimmel, A. Shani, A. Greenberger, Bernadette Dubos, Shoshana Yathom, Y. Zilberstein, P. Cwielong, P. Lazarovici, Jaacov Katan, M. Malka, Rivka Eliyahu, Ilan Chet, S. Gothilf, Y. Bashan, R. Baker, E. Dunkelblum, Y. Golan, S. Tam, P. Cornuet, Yigal Cohen, F. Jailloux, Rut Ideses, H. Lecoq, R. Delon, G. Massonie, J. Dunez, Y. Szivos, A. Hüttermann, P. Camporota, A. Grinstein, Joseph M. Bové, P. Davet, P. Maison, B. A. Peleg, A. Berville, J. T. Klug, M. Trottet, Jacqueline Skorka, J. Bulit, A. Rochel, A. Hefetz, M. Pitrat, I. Bar-Zakkai, Y. Okon, Michele Paillard, E. Breuer, G. E. Harman, A. Sivan, R. G. Kenneth, M. Chen, G. Doussinault, R. Delbos, J. Renaudin, D. Netzer, D. Becker, M. Platt, Annegret Haars, Ph Auriau, M. Sternlicht, Y. Yom-Tov, F. Rapilly, M. Artigues, Francoise Dosba, R. Dahan, P. Schiltz, E. Gurevitz, R. Ikan, Rina Ben Jaacov, R. Kagan, Shabtai Cohen, J. Jahier, C. Mouches, Z. Eyal, Yigal Elad, M. Kehat, and A. Gera

Subjects
0106 biological sciences, 010602 entomology, Insect Science, Ecology (disciplines), Physiology, Library science, Plant Science, Biology, Plant disease resistance, 01 natural sciences, Selection (genetic algorithm), 010606 plant biology & botany
Published
1982
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34. Immunoenzymatic techniques for the detection of Phoma exigua in infected potato tissues

Author

J. Dunez, M. Aguelon, Unité mixte de recherche santé végétale, and Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)

Subjects
0106 biological sciences, Antiserum, 0303 health sciences, Industrial crop, biology, Phoma tracheiphila, medicine.drug_class, Inoculation, [SDV]Life Sciences [q-bio], fungi, Phoma exigua, biology.organism_classification, Monoclonal antibody, 01 natural sciences, 3. Good health, Microbiology, 03 medical and health sciences, [SDE]Environmental Sciences, Exigua, medicine, Agronomy and Crop Science, Mycelium, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience; Antisera to Phoma exigua var. foveata and var. exigua were prepared by injecting rabbits and mice with protein solutions from mycelium. Specific antibodies were isolated and immunoenzymatic techniques (double antibody sandwich ELISA and indirect ELISA) were used to test for the fungus in inoculated tubers and sprouts and in stems grown from these tubers. The fungus was detected in these different tissues, with var. foveata being more aggressive, demonstrating the applicability and sensitivity of the techniques. The antibodies produced to the two varieties of the fungus were not specific to their own varieties. They also reacted with Phoma tracheiphila but did not react with several other common potato pathogens. Preliminary results obtained with antibodies from mouse ascite liquid suggest the possibility of producing specific monoclonal antibodies.

Published
1984
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42. Analysis of nine isolates of almond mosaic

Author

R. Bernhard, M. Lansac, G. Detienne, J. Dunez, Station de recherches fruitières, Institut National de la Recherche Agronomique (INRA), UR 0418 Station de recherches fruitières, Villenave-d'Ornon, and Unité de recherches Espèces Fruitières et Vigne (UREFV)

Subjects
0106 biological sciences, viruses, [SDV]Life Sciences [q-bio], fungi, food and beverages, Mosaic (geodemography), 04 agricultural and veterinary sciences, Horticulture, Biology, 01 natural sciences, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, 010606 plant biology & botany
Abstract

International audience; Nine almond tree cultivars originating from the Mediterranean area and cultivated in South France, express mosaic symptoms on the leaves. The purpose of this study is to investigate the possible virus origin of almond mosaic. The nine isolates were observed during four years and indexed on woody and herbaceous indicators. The immuno-enzymatic ELISA procedure was applied for prunus necrotic ring spot virus (PNRSV), prune dwarf virus (PDV) and apple chlorotic leaf spot virus (CLSV). It appears that mosaic symptoms in almond can result from infection by PNRSV, PDV and CLSV, but it also appears in the absence of these viruses and can be induced by another virus which, so far, has not been identified and could be distantly related to some ILAR viruses.

Published
1980
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43. Interest in Serological Techniques for the Detection and Identification of Phoma Exigua, the Agent of Potato Gangrene

Author

J. Dunez, C. Kerlan, M. Aguelon, L. Hingand, Bernard Jouan, and S. Le Coz

Subjects
biology, fungi, food and beverages, Early detection, Phoma exigua, Fungus, biology.organism_classification, Serology, Microbiology, Rapid identification, Potato gangrene, Brown pigment, Botany, Exigua
Abstract

Potato gangrene is an important storage disease of potatoes. The responsible fungus is Phoma exigua. The two varieties of this fungus, P. exigua and P. foveata can induce necrotic lesions on tubers, but P. exigua var. foveata is responsible for most damage. Early detection of infection and precise and rapid identification of the fungus variety are of major interest. So far, differentiation of the varieties have relied on biochemical tests and specific production of a brown pigment in the P. foveata cultures. Our attempts were to develop serological tests both for the detection of P. exigua and differentiation of the 2 varieties.

Published
1988
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44. Virus infection of walnuts in France

Author

M. Lansac, J. Dunez, E. Germain, F. Dosba, C. Kerlan, R. Delbos, UR 0418 Station de recherches fruitières, Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), Station de recherches fruitières, and Unité de recherches Espèces Fruitières et Vigne (UREFV)

Subjects
0106 biological sciences, 0303 health sciences, 03 medical and health sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, Horticulture, Biology, 01 natural sciences, Virology, Virus, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience; In the past 20 years, Juglans nigra has been introduced in the new French plantations of Walnut as a dwarfing rootstock able to induce a rapid fruiting. Presently in France, there are 2 millions trees, half growing as separate trees. The percentage of trees grafted in orchards on J. nigra varies with the region from 5 % to 95 % with an average of 15–20 % : most trees grafted on J. nigra are less than 20 years old. Black line (BL) symptom at the union was observed on J. regia grafted on J. nigra as it was described in the USA on trees grafted on J. hindsii or Paradox. The black line symptom was observed on 15–20 years old trees and seems to appear earlier when the trees grow in poor soils. Trees die within 2–4 years after the first BL symptom appears. BL symptom was never observed on J. regia grafted on J. regia. Cherry leaf roll virus was isolated from trees grafted on J.nigra with BL symptoms but was also present in some symptomless trees without any symptoms. Such symptomless infections were also detected on trees grafted on J. regia. The virus was identified by bioassay on herbaceous plants and serology (ELISA). Presence of CLRV was demonstrated on 6 varieties. The French Walnut CLRV isolates have been successfully transmitted onto the peach seedlings GF 305 which show a stunt reaction very similar to that observed when infected with CLRV strains from other origins. The virus is seed borne and the percentage of infected seedlings in J. regia is 5–20 %. From the serological tests, the French isolates appear to be closely related to the US Walnut isolates and the Elderberry strain of Cherry leaf roll and are also related to the Walnut ring spot and Walnut yellow vein viruses described in Italy.

Published
1982
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46. Identification and comparison of different strains od apple chlorotic leaf spot virus and possibilities of cross protection

Author

J. Dunez, C. Marenaud, R. Bernhard, INRA : UR419, Institut National de la Recherche Agronomique (INRA), Unité de recherches Espèces Fruitières et Vigne (UREFV), Station d'Arboriculture Fruitière, and ProdInra, Migration

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], 04 agricultural and veterinary sciences, Horticulture, Biology, 01 natural sciences, Apple chlorotic leaf spot virus, [SDV] Life Sciences [q-bio], Botany, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Identification (biology), ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany
Abstract

International audience

Published
1976

47. Detection of Chrysanthemum stunt and chlorotic mottle viroids by slab gel electrophoresis

Author

J. Dunez, J. C. Bachelier, M. Monsion, C. Faydi, G. Macquaire, UR 0420 - Station de pathologie végétale, Laboratoire de pathologie forestière, and Institut National de la Recherche Agronomique (INRA)-Institut National de la Recherche Agronomique (INRA)

Subjects
Gel electrophoresis, viruses, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Slab, medicine, food and beverages, Mottle, Horticulture, Biology, medicine.disease, Virology
Abstract

The two Chrysanthemum viroids, Chrysanthemum stunt (CSV) and Chrysanthemum chlorotic mottle (CChMV) are detected by polyacrylamide gel electrophoresis ; the electrophoretic assay is an adaptation to slab gels (PASGE) of the Morris and Smith's procedure previously applied to Potato spindle tuber viroid. Use of ethidium bromide staining, specific of double stranded nucleic acids, makes the method much more sensitive than after toluidine blue 0 staining or by U.V. scanning of cylindrical gels.Compared to the biological indexing the technique appears highly sensitive : this biochemical test allows CSV detection in samples as small as 10 mg plant material.By using PASGE the presence of viroids can be noticed earlier after infection than with the normal biological indexing : viroid RNA can be observed in plants which do not show any symptom.The importance of sampling is emphasized. Viroids are detected in any part of the infected Chrysanthemum plants. Nevertheless, viroids concentration is higher in the tips and the young leaves which are the best material for indexing.This technique has been applied to a large number of samples. It is simple, rapid, highly sensitive and reliable and consequently seems to be very appropriate to a large scale indexing.

Published
1980
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48. European Handbook of Plant Diseases

Author

J. Dunez, I. M. Smith, D. H. Phillips, S. A. Archer, and R. A. Lelliott

Subjects
Dothideales, Helotiales, biology, Ophiostomatales, viruses, Botany, Plant pathology, Ustilaginales, Eurotiales, biology.organism_classification, Diaporthales, Chytridiomycetes, Microbiology
Abstract

Viruses I: General: Major virus groups Viruses II: Minor virus groups and virus complexes Viruses III: Ungrouped viruses and virus complexes Viruses IV: Diseases assumed to be due to viruses Mollicutes and rickettsia-like organisms Bacteria I: Pseudomonas and Xanthomonas Bacteria II: Agrobacterium, Corynebacterium, Erwinia and other bacteria Oomycetes Chytridiomycetes, Plasmodiophoromycetes, Zygomycetes Ascomycetes I: General: Endomycetales, Taphrinales, Eurotiales, Erysiphales Ascomycetes II: Clavicipitales, Hypocreales Ascomycetes III: Ophiostomatales, Diaporthales, Polystigmatales, Sphaeriales, Diatrypales, Coryneliales Ascomycetes IV: Dothideales Ascomycetes V: Rhytismatales, Caliciales, Pezizales, Caliciales Helotiales Basidiomycetes I: Ustilaginales Basidiomycetes II: Uredinales Basidiomycetes III: Hymenomycetes Pathogen index Host index

Published
1988
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49. [Satellite RNA (RNA3) of tomato black ring virus is found with one of the 2 major RNAs (RNA2) in a new capsid nucleoprotein]

Author

B, Doz, J, Dunez, and J M, Bove

Subjects
Viral Proteins, Capsid, Nucleoproteins, Ribonucleoproteins, RNA, Viral, Plant Viruses
Abstract

Tomato Black Ring Virus (TBRV) like other NEPOviruses posseses two nucleoproteins M and B and two major RNAs, RNA1 and RNA2 respectively distributed in B and M. A new nucleoprotein has just been discovered and comprises one molecule of RNA2 associated with one molecule of RNA3. RNA3 is a small RNA of molecular weight 500,000 d considered to be a satellite RNA. Its level appears to depend on the infection stage, local or systemic. RNA3 is able to modify the relative proportions of nucleoproteins M and B and their respective RNAs. The satellite RNA, might be part of the genome and represent a monocistronic mRNA for protein capsid synthesis. However it seems perhaps more tempting to correlate TBRV-RNA3 with satellite RNA5 of certain strains of Cucumber mosaic virus.

Published
1977

50. Preliminary studies on the antagonism between strains of plum pox virus

Author

J. Dunez, C. Kerlan, P. Maison, M. Lansac, UR 0418 Station de recherches fruitières, Villenave-d'Ornon, Institut National de la Recherche Agronomique (INRA), Station de recherches fruitières, and Unité de recherches Espèces Fruitières et Vigne (UREFV)

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Pox virus, 04 agricultural and veterinary sciences, Horticulture, Biology, Antagonism, 01 natural sciences, Virology, 010606 plant biology & botany
Abstract

International audience; The cross protecting effect of some mild strains of plum pox virus was checked in preliminary experiments carried out in the greenhouse. These mild strains were inoculated by chip-budding. The severe challenge strain was inoculated by aphid transmission using the peach green aphid, Myzus persicae. The results indicate a protective effect of the mild strains. Protection varies with, the nature of the mild strain but in each case the protected trees did not show the severe symptoms usually induced by the challenging severe strain. Symptoms appeared intermediate in intensity and the incubation period was longer than in the unprotected plants

Published
1980
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