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Vibrio parahaemolyticus is a kind of food-borne pathogenic bacterium, which can seriously infect food, especially seafood causing gastroenteritis and other disease. We studied the global proteome responses of V. parahaemolyticus under cold stress by nano-liquid chromatography-tandem mass spectrometry to improve the present understanding of V. parahaemolyticus proteomics events under cold stress. A total of 1151 proteins were identified and 101 proteins were differentially expressed, of which 69 were significantly up-regulated and 32 were downregulated. Functional categorization of these proteins revealed distinct differences between cold-stressed and control cells. These proteins were grouped into 21 functional categories by the clusters of orthologous groups (COG) analysis. The most of up-regulated proteins were functionally categorized as nucleotide transport and metabolism, transcription, function unknown, and defense mechanisms. These up-regulated proteins play an important role under cold stress. [ABSTRACT FROM AUTHOR]

Vibrio parahaemolyticus is a common foodborne bacterial pathogen, which survives in cold environments and is sometimes difficult to culture. Fatty acid analysis under cold stress was conducted for several V. parahaemolyticus strains using gas chromatography/mass spectrometry, and the results were compared with those of the controls. All the fatty acid profiles obtained were visualized by multidimensional scaling ( MDS) and self-organized map ( SOM). It was observed that the fatty acid profiles of V. parahaemolyticus substantially changed under cold stress. The percentage of methyl palmitate remarkably decreased and that of methyl palmitoleate (except for two strains) and methyl oleate increased. These findings demonstrate the role of fatty acids in cold stress. The changes in the fatty acid profiles illustrated by MDS and SOM could differentiate strains under cold stress from the controls and can potentially lead to a method of detecting injured cold-stressed V. parahaemolyticus. [ABSTRACT FROM AUTHOR]

The development of fluvial systems in East Asia is closely linked to the evolving topography following India-Eurasia collision. Despite this, the age of the Yangtze River system has been strongly debated, with estimates ranging from 40 to 45 Ma, to a more recent initiation around 2 Ma. Here, we present 40Ar/39Ar ages from basalts interbedded with fluvial sediments from the lower reaches of the Yangtze together with detrital zircon U-Pb ages from sand grains within these sediments. We show that a river containing sediments indistinguishable from the modern river was established before ~23 Ma. We argue that the connection through the Three Gorges must postdate 36.5 Ma because of evaporite and lacustrine sedimentation in the Jianghan Basin before that time. We propose that the present Yangtze River system formed in response to regional extension throughout eastern China, synchronous with the start of strike-slip tectonism and surface uplift in eastern Tibet and fed by strengthened rains caused by the newly intensified summer monsoon. [ABSTRACT FROM AUTHOR]

Objective: To explore detecting methods and quality control standard system for hepatitis A virus( HAV) in water for fruits and vegetables production., Methods: 100 μL of coliphage MS2( 2. 5 × 10~9 pfu / mL) was added into each water sample, and positively charged membrane filter method was used to capture virus. The virus extraction efficiency of each sample can be calculated according to standard curve of coliphage. Then the quality control system and real-time RT-PCR method were established., Results: This research compared the extraction efficiency of HAV and coliphage MS2 which were added into the water samples at the same time. The extraction efficiency of HAV was from 1. 24% to 32. 68%, and coliphage MS2 1. 64% to 14. 24%. The efficiency met the requirements of ISO / TS 15216-2-2013. Meanwhile, the HAV in 30 water samples were detected, and one was positive. The extraction efficiency of coliphage MS2 was from1. 24% to 24. 19%, and the standard deviation was 0. 0612., Conclusion: This research establish a quality control system to ensure the quality of test results.

Objective: Bacterial strain F5-1 isolated from the Homarus americanus was characterized and its changes in membrane fatty acid composition in response to low temperature were also studied., Methods: The physiological and biochemical characteristics were carried out by using VITEK 2 compact automated microbiology system. The 16S rRNA gene was sequenced and subjected to phylogenetic analysis. Fatty acids were detected by gas chromatography-mass spectrometry (GC-MS)., Results: Strain F5-1 was Gram-negative and susceptible to the vibriostatic agent O/129. Strain F5-1 was resistant to Penicillin. The isolated strain exhibited the highest levels of 99% probability to Vibrio metschnikovii based on the conventional physiological test. The sequence analysis of 16S rRNA gene of F5-1 isolation and comparison with that of other related vibrios showed that F5-1 was very close to V. metschnikovii (GenBank No. HQ658055). The similarity was 99%. The major fatty acids were C12:0, C14:0, C16:0 and C16:1 (n-7). Palmitoleic acid was the dominant unsaturated fatty acids. The major change in fatty acid composition occurred in response to low temperature, with an increase in palmitoleic acid from 34% to 40%., Conclusion: Bacterial strain F5-1 isolated from Homarus americanus was identified as V. metschnikovii and was sensitive to multiple drugs. The fatty acid composition of F5-1 was different from V. metschnikovii isolated from a drinking water reservoir near Vladivostok City in the Russia Far East. Results of this study indicated that environmental conditions allowed modulation of the fatty acid composition of V. metschnikovii.