90 results on '"JIN-PENG ZHANG"'
Search Results
2. Unraveling the genetic basis of grain number-related traits in a wheat-Agropyron cristatum introgressed line through high-resolution linkage mapping
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Yun-Feng Xu, Fei-Fei Ma, Jin-Peng Zhang, Hong Liu, Li-Hui Li, and Diao-Guo An
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Grain number per spike ,Grain number per spikelet ,Quantitative trait locus (QTL) ,High-density linkage map ,Wheat-Agropyron cristatum germplasm ,Botany ,QK1-989 - Abstract
Abstract Background Grain number per spike (GNS) is a pivotal determinant of grain yield in wheat. Pubing 3228 (PB3228), a wheat-Agropyron cristatum germplasm, exhibits a notably higher GNS. Results In this study, we developed a recombinant inbred line (RIL) population derived from PB3228/Gao8901 (PG-RIL) and constructed a high-density genetic map comprising 101,136 loci, spanning 4357.3 cM using the Wheat 660 K SNP array. The genetic map demonstrated high collinearity with the wheat assembly IWGSC RefSeq v1.0. Traits related to grain number and spikelet number per spike were evaluated in seven environments for quantitative trait locus (QTL) analysis. Five environmentally stable QTLs were detected in at least three environments. Among these, two major QTLs, QGns-4A.2 and QGns-1A.1, associated with GNS, exhibited positive alleles contributed by PB3228. Further, the conditional QTL analysis revealed a predominant contribution of PB3228 to the GNS QTLs, with both grain number per spikelet (GNSL) and spikelet number per spike (SNS) contributing to the overall GNS trait. Four kompetitive allele-specific PCR (KASP) markers that linked to QGns-4A.2 and QGns-1A.1 were developed and found to be effective in verifying the QTL effect within a diversity panel. Compared to previous studies, QGns-4A.2 exhibited stability across different trials, while QGns-1A.1 represents a novel QTL. The results from unconditional and conditional QTL analyses are valuable for dissecting the genetic contribution of the component traits to GNS at the individual QTL level and for understanding the genetic basis of the superior grain number character in PB3228. The KASP markers can be utilized in marker-assisted selection for enhancing GNS. Conclusions Five environmentally stable QTLs related to grain number and spikelet number per spike were identified. PB3228 contributed to the majority of the QTLs associated with GNS.
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- 2023
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3. Observation of GRB 221009A Early Afterglow in X-Ray/Gamma-Ray Energy Bands
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Chao Zheng, Yan-Qiu Zhang, Shao-Lin Xiong, Cheng-Kui Li, He Gao, Wang-Chen Xue, Jia-Cong Liu, Chen-Wei Wang, Wen-Jun Tan, Wen-Xi Peng, Zheng-Hua An, Ce Cai, Ming-Yu Ge, Dong-Ya Guo, Yue Huang, Bing Li, Ti-Pei Li, Xiao-Bo Li, Xin-Qiao Li, Xu-Fang Li, Jin-Yuan Liao, Cong-Zhan Liu, Fang-Jun Lu, Xiang Ma, Rui Qiao, Li-Ming Song, Jin Wang, Ping Wang, Xi-Lu Wang, Yue Wang, Xiang-Yang Wen, Shuo Xiao, Yan-Bing Xu, Yu-Peng Xu, Zhi-Guo Yao, Qi-Bing Yi, Shu-Xu Yi, Yuan You, Fan Zhang, Jin-Peng Zhang, Peng Zhang, Shu Zhang, Shuang-Nan Zhang, Yan-Ting Zhang, Zhen Zhang, Xiao-Yun Zhao, Yi Zhao, and Shi-Jie Zheng
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Gamma-ray bursts ,Astrophysics ,QB460-466 - Abstract
The early afterglow of a gamma-ray burst (GRB) can provide critical information on the jet and progenitor of the GRB. The extreme brightness of GRB 221009A allows us to probe its early afterglow in unprecedented detail. In this Letter, we report comprehensive observation results of the early afterglow of GRB 221009A (from T _0 +660 s to T _0 +1860 s, where T _0 is the Insight-HXMT/HE trigger time) in X-ray/gamma-ray energy band (from 20 keV to 20 MeV) by Insight-HXMT High Energy X-ray Telescope, GECAM-C, and Fermi/Gamma-ray Burst Monitor. We find that the spectrum of the early afterglow in 20 keV–20 MeV can be well described by a cutoff power law with an extra power law that dominates the low- and high-energy bands, respectively. The cutoff power law E _peak is ∼30 keV, and the power-law photon index is ∼1.8 throughout the early afterglow phase. By fitting the light curves in different energy bands, we find that a significant achromatic break (from keV to TeV) is required at T _0 + ${1246}_{-26}^{+27}$ s (i.e., 1021 s since the afterglow starting time T _AG = T _0 +225 s), providing compelling evidence of a jet break. Interestingly, both the pre-break and post-break decay slopes vary with energy, and these two slopes become closer in the lower energy band, making the break less identifiable. Intriguingly, the spectrum of the early afterglow experienced a slight hardening before the break and a softening after the break. These results provide new insights into the physics of this remarkable GRB.
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- 2024
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4. Genetic effects of Agropyron cristatum 2P chromosome translocation fragments in a wheat background
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Shi-rui XU, Bo JIANG, Hai-ming HAN, Xia-jie JI, Jin-peng ZHANG, Sheng-hui ZHOU, Xin-ming YANG, Xiu-quan LI, Li-hui LI, and Wei-hua LIU
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wheat–Agropyron cristatum 2P chromosome translocation lines ,flag leaf ,spikelet density ,genetic effects ,Agriculture (General) ,S1-972 - Abstract
Agropyron cristatum (2n=4x=28, PPPP) is a wild relative of common wheat which contains a large number of desirable genes that can be exploited for wheat improvement. Wheat–A. cristatum 2P alien translocation lines exhibit many desirable traits, such as small flag leaves, a high spikelet number and density, and a compact plant type. An agronomic trait evaluation and a genetic analysis were carried out on translocation lines and backcross populations of these lines carrying different translocation fragments. The results showed that a translocation fragment from 2PT-3 (2PL) reduced the length of the flag leaves, while translocation fragments from 2PT-3 (2PL) and 2PT-5 (2PL (0.60–1.00)) reduced the width of the flag leaves. A translocation fragment from 2PT-13 (2PS (0.18–0.36)) increased the length and area of the flag leaves. Translocation fragments from 2PT-3 (2PL) and 2PT-8 (2PL (0.86–1.00)) increased the density of spikelets. Translocation fragments from 2PT-7 (2PL (0.00–0.09)), 2PT-8 (2PL (0.86–1.00)), 2PT-10 (2PS), and 2PT-13 (2PS (0.18–0.36)) reduced plant height. This study provides a scientific basis for the effective utilization of wheat–A. cristatum translocation lines.
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- 2023
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5. Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat
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Hong-xin CHEN, Hai-ming HAN, Qing-feng LI, Jin-peng ZHANG, Yu-qing LU, Xin-ming YANG, Xiu-quan LI, Wei-hua LIU, and Li-hui LI
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common wheat ,Agropyron cristatum ,derivatives ,in situ ,hybridization ,molecular markers ,Agriculture (General) ,S1-972 - Abstract
Agropyron cristatum, a wild relative of common wheat (Triticum aestivum L.), provides many desirable genetic resources for wheat improvement, such as tolerance to cold, drought, and disease. To transfer and utilize these desirable genes, in this study, two wheat-A. cristatum derivatives II-13 and II-23 were identified and analyzed. We found that the number of root tip cell chromosomes was 44 in both II-13 and II-23, but there were four and six P genome chromosomes in II-13 and II-23, respectively, based on genomic in situ hybridization (GISH). The chromosome configurations of II-13 and II-23 were both 2n=22II by the meiotic analysis of pollen mother cells (PMCs) at metaphase I, indicating that there were two and three pairs of P chromosomes in II-13 and II-23, respectively. Notably, wheat chromosome 7D was absent in derivative line II-13 while II-23 lacked chromosomes 4B and 7A based on SSR analysis combining fluorescence in situ hybridization (FISH) analysis with pAs1 and pSc119.2 as probes. Chromosomes 2P and 7P were detected in both II-13 and II-23. Another pair of P genome chromosomes in II-23 was determined to be 4P based on expressed-sequences tags-sequence tagged sites (EST-STS) markers specific to A. cristatum and FISH with probes pAcTRT1 and pAcpCR2. Overall, these results suggest that II-13 was a 7P (7D) substitution line with one pair of additional 2P chromosomes and II-23 was a multiple 4P (4B), 7P (7A) substitution line with one pair of additional 2P chromosomes. Moreover, we obtained six alien disomic addition lines and five alien disomic substitution lines by backcrossing. These new materials will allow desirable genes from A. cristatum to be used in common wheat.
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- 2018
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6. Genetic characteristics of a wheat founder parent and a widely planted cultivar derived from the same cross
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Li-fang CHANG, Hui-hui LI, Xiao-yang WU, Yu-qing LU, Jin-peng ZHANG, Xin-ming YANG, Xiu-quan LI, Wei-hua LIU, and Li-hui LI
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wheat ,founder parents ,widely planted cultivars ,SNP ,Agriculture (General) ,S1-972 - Abstract
Founder parents have contributed significantly to the improvement of wheat breeding and production. In order to investigate the genetic characteristics of founder parents and widely planted cultivars, Mazhamai (M), Biyumai (B) and six sibling lines (BM1–6) derived from the cross M×B were phenotyped for eight yield-related traits over multiple years and locations and genotyped using the the wheat 90K single nucleotide polymorphism (SNP) assay. BM4 has been used as a founder parent, and BM1 has been widely planted, whereas BM2, 3, 5, and 6 have not been used extensively for breeding or planting in China. Phenotypic comparisons revealed that BM4 and BM1 displayed a better overall performance than the other sibling lines. BM1 showed higher thousand-grain weight than BM4, whereas BM4 exhibited lower coefficient of variation for most of the yield-related traits across different years and locations, indicating that BM4 was widely adaptable and more stable in different environments. SNP analysis revealed that BM4 and BM1 inherited similar proportions of the M genome but are dissimilar to BM2, 3, 5, and 6. Both BM1 and BM4 have specific alleles that differ from the other BM lines, and most of these alleles are concentrated in specific chromosomal regions that are found to associate with favorable QTLs, these SNPs and their surrounding regions may carry the genetic determinants important for the superior performance of the two lines. But BM4 has more genetic diversity than BM1 with more specific alleles and pleiotropic regions, indicating that the genome of BM4 may be more complex than the other sibling lines and has more favorable gene resources. Our results provide valuable information that can be used to select elite parents for wheat and self-pollinating crop breeding.
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- 2018
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7. Novel and favorable genomic regions for spike related traits in a wheat germplasm Pubing 3504 with high grain number per spike under varying environments
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Dan CHEN, Xiao-yang WU, Kuo WU, Jin-peng ZHANG, Wei-hua LIU, Xin-ming YANG, Xiu-quan LI, Yu-qing LU, and Li-hui LI
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wheat ,high GNPS germplasm ,QTL mapping ,genomic region ,Agriculture (General) ,S1-972 - Abstract
Grain number per spike (GNPS) is a major factor in wheat yield breeding. A new wheat germplasm Pubing 3504 shows superior features in spike traits. To elucidate the genetic basis of spike and yield related traits in Pubing 3504, 282 F2:3 families were generated from the cross Pubing 3504×Jing 4839, and seven spike and yield related traits, including GNPS, spike length (SL), kernel number per spikelet (KPS), spikelet number per spike (SNS), thousand-grain weight (TGW), spike number per plant (SNP), and plant height (HT) were investigated. Correlation analysis indicated significant positive correlations between GNPS and spike-related traits, including KPS, SNS, and SL, especially KPS. A genetic map was constructed using 190 polymorphic simple sequence repeat (SSR), expressed sequence tag (EST)-SSR, and sequence-tagged-site (STS) markers. For the seven traits measured, a total of 37 quantitative trait loci (QTLs) in a single-environment analysis and 25 QTLs in a joint-environment analysis were detected. Additive effects of 70.3% (in a single environment) and 57.6% (in a joint environment) of the QTLs were positively contributed by Pubing 3504 alleles. Five important genomic regions on chromosomes 1A, 4A, 4B, 2D, and 4D could be stably detected in different environments. Among these regions, the marker interval Xmag834–Xbarc83 on the short arm of chromosome 1A was a novel important genomic region that included QTLs controlling GNPS, KPS, SNS, TGW, and SNP with stable environmental repeatability. This genomic region can improve the spike trait and may play a key role in improving wheat yield in the future. We deduced that this genomic region was vital to the high GNPS of Pubing 3504.
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- 2017
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8. Gene and protein expression profiling analysis of young spike development in large spike wheat germplasms
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Dan CHEN, Jin-peng ZHANG, Wei-hua LIU, Xiao-yang WU, Xin-ming YANG, Xiu-quan LI, Yu-qing LU, and Li-hui LI
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wheat ,high grain number per spike ,spike development ,expression profiling ,Agriculture (General) ,S1-972 - Abstract
The wheat grain number per spike (GNPS) is a major yield-limiting factor in wheat-breeding programs. Germplasms with a high GNPS are therefore valuable for increasing wheat yield potential. To investigate the molecular characteristics of young spike development in large-spike wheat germplasms with high GNPS, we performed gene and protein expression profiling analysis with three high-GNPS wheat lines (Pubing 3228, Pubing 3504 and 4844-12) and one low-GNPS control variety (Fukuho). The phenotypic data for the spikes in two growth seasons showed that the GNPS of the three large-spike wheat lines were significantly higher than that of the Fukuho control line. The Affymetrix wheat chip and isobaric tags for relative and absolute quantitation-tandam mass spectrometry (iTRAQ-MS/MS) technology were employed for gene and protein expression profiling analyses of young spike development, respectively, at the floret primordia differentiation stage. A total of 598 differentially expressed transcripts (270 up-regulated and 328 down-regulated) and 280 proteins (122 up-regulated and 158 down-regulated) were identified in the three high-GNPS lines compared with the control line. We found that the expression of some floral development-related genes, including Wknox1b, the AP2 domain protein kinase and the transcription factor HUA2, were up-regulated in the high-GNPS lines. The expression of the SHEPHERD (SHD) gene was up-regulated at both the transcript and protein levels. Overall, these results suggest that multiple regulatory pathways, including the CLAVATA pathway and the meristem-maintaining KNOX protein pathway, take part in the development of the high-GNPS phenotype in our wheat germplasms.
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- 2016
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9. Experimental Study on Basic Mechanical Properties of Steel Fiber-Reinforced Siliceous Wet Shotcrete
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Xu Yan, Li-min Liu, Jin-peng Zhang, Yan-hui Li, and He Wang
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Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
In order to solve the problem of low strength and easy cracking of shotcrete in permanent support of tunnel single shell lining, the effects of steel fiber-reinforced siliceous on mechanical properties of wet shotcrete were investigated by the tests of compressive strength, splitting tensile strength, flexural strength, shear strength, and bending toughness. The strength and the cracking resistance mechanism of steel fiber-reinforced siliceous wet shotcrete were analyzed by the bending toughness evaluation method. The results show that (1) the steel fiber-reinforced siliceous can improve splitting tensile, flexural, and shear capacity of the shotcrete, and the maximum growth rates were 77.42%, 72.73%, and 98.31%. The steel fiber plays a major role, and silica fume plays a subsidiarity role. (2) The effect of different types and contents of steel fibers on the compressive strength of wet shotcrete are not obvious. (3) The strengthening effect of shear undaform steel fiber on concrete is obviously better than that of shear flat steel fiber. (4) The flexural toughness of wet shotcrete with the silica content of 10% and the shear steel fiber of 60 kg/m3 is the best.
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- 2018
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10. Inheritance and Availability of High Grain Number Per Spike in Two Wheat Germplasm Lines
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Dan CHEN, Jin-peng ZHANG, Jian-sheng WANG, Xin-ming YANG, Wei-hua LIU, Ai-nong GAO, Xiu-quan LI, and Li-hui LI
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wheat ,high grain number per spike ,inheritance ,yield breeding ,Agriculture (General) ,S1-972 - Abstract
Grain number per spike (GNPS) is a major factor in wheat yield breeding. The development of high GNPS germplasm is widely emphasized in wheat-yield breeding. This paper reported two high GNPS wheat germplasm lines, Pubing 3228 and Pubing 3504, which had a stable and wide adaptability to different ecological regions. By exploring a nested cross design with reciprocals using Pubing 3228 or Pubing 3504 as a common parent and investigating the GNPS phenotypes of F1 hybrids in 2007-2008 and F2 populations in 2008-2009 of different cross combinations, the narrow-sense GNPS heritability was up to 49.58 and 52.23%, respectively. Genetic model analysis predictions suggested that GNPS in Pubing 3228 and Pubing 3504 was mainly controlled by additive genetic effects. Correlation analysis results between GNPS and 1 000-kernel weight (TKW) of F2 populations showed that TKW was not influenced with the increase of GNPS. The good coordination among three yield components of spike number per plant (SNPP), GNPS, and TKW in the F2 segregating population implied that selection of good candidate individuals in breeding programs would be relatively straightforward. Overall, our results indicated that Pubing 3228 and Pubing 3504 are two potential germplasm lines for yield improvement of GNPS in pedigree selection of wheat breeding.
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- 2012
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11. Developmental genetics of the perianthless flowers and bracts of a paleoherb species, Saururus chinensis.
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Yin-He Zhao, Zachary Larson-Rabin, Guo-Ying Wang, Michael Möller, Cheng-Yun Li, Jin-Peng Zhang, Hong-Tao Li, and De-Zhu Li
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Medicine ,Science - Abstract
Saururus chinensis is a core member of Saururaceae, a perianthless (lacking petals or sepals) family. Due to its basal phylogenetic position and unusual floral composition, study of this plant family is important for understanding the origin and evolution of perianthless flowers and petaloid bracts among angiosperm species. To isolate genes involved in S. chinensis flower development, subtracted floral cDNA libraries were constructed by using suppression subtractive hybridization (SSH) on transcripts isolated from developing inflorescences and seedling leaves. The subtracted cDNA libraries contained a total of 1,141 ESTs and were used to create cDNA microarrays to analyze transcript profiles of developing inflorescence tissues. Subsequently, qRT-PCR analyses of eight MADS-box transcription factors and in situ hybridizations of two B-class MADS-box transcription factors were performed to verify and extend the cDNA microarray results. Finally, putative phylogenetic relationships within the B-class MADS-box gene family were determined using the discovered S. chinensis B-class genes to compare K-domain sequences with B genes from other basal angiosperms. Two hundred seventy-seven of the 1,141 genes were found to be expressed differentially between S. chinensis inflorescence tissues and seedling leaves, 176 of which were grouped into at least one functional category, including transcription (14.75%), energy (12.59%), metabolism (9.12%), protein-related function (8.99%), and cellular transport (5.76%). qRT-PCR and in situ hybridization of selected MADS-box genes supported our microarray data. Phylogenetic analysis indicated that a total of six B-class MADS-box genes were isolated from S. chinensis. The differential regulation of S. chinensis B-class MADS-box transcription factors likely plays a role during the development of subtending bracts and perianthless flowers. This study contributes to our understanding of inflorescence development in Saururus, and represents an initial step toward understanding the formation of petaloid bracts in this species.
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- 2013
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12. Application of PSO-GA&CGA in Sea-Clutter Doppler Spectrum Modeling.
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Shu-Bin Zhang, Tingting Ji, Wen-Yuan Wang, and Jin-Peng Zhang
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- 2020
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13. Deep learning for inversion of significant wave height based on actual sea surface backscattering coefficient model.
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Tao Wu 0017, Yunhua Cao, Zhensen Wu, Jiaji Wu, Tan Qu, and Jin-Peng Zhang
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- 2020
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14. Study on the Relationship between Microstructure and Macroproperties of Rubber Asphalt at Different Activation Temperatures
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Guang-tai Zhang, Bao Yi, Xiao-xu Zhang, and Jin-peng Zhang
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- 2021
15. Design and application of QR code positioning for orbital inspection robot in high-voltage substations
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Lan-xi Shi, Hong-yu Ni, Jin-peng Zhang, Zu-rong Lin, and Wen-xu Yan
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- 2022
16. Cypermethrin induces Sertoli cell apoptosis through mitochondrial pathway associated with calcium
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Jin-Peng Zhang, Mei-Rong Zhang, Rui Zhang, Yue Yu, Lu-Shan Wang, Zhen Ding, Qi Wang, Zheng Li, Li-Chun Xu, and Heng-Xue Wang
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Paper ,0301 basic medicine ,Calmodulin ,biology ,Chemistry ,Health, Toxicology and Mutagenesis ,Toxicology ,Sertoli cell ,Cell biology ,Cypermethrin ,03 medical and health sciences ,chemistry.chemical_compound ,Cytosol ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Apoptosis ,Ca2+/calmodulin-dependent protein kinase ,biology.protein ,medicine ,Inner mitochondrial membrane ,030217 neurology & neurosurgery ,Intracellular - Abstract
Cypermethrin, one kind of pyrethroid pesticides, has been shown to act as endocrine-disrupting chemicals (EDCs). The purpose of this study was to explore the roles of Sertoli cell apoptosis through mitochondrial pathway associated with calcium (Ca2+) in cypermethrin-induced male reproductive toxicology. The mouse Sertoli cells TM4 were cultured with 0 μM, 10 μM, 20 μM, 40 μM and 80 μM of cypermethrin. We used flow cytometry, Fluo-4 AM, western blot and JC-1 Assay Kit to examine apoptosis, intracellular Ca2+, expressions of mitochondrial apoptotic pathway-related proteins and mitochondrial membrane potential. We found cypermethrin increased apoptosis rate of TM4 cells significantly and with a significant increase in intracellular Ca2+ concentration. Cypermethrin significantly decreased the protein expressions of cytosolic B-cell lymphoma-2 (Bcl-2) and mitochondrial cytochrome c (Cyt-c). The protein expressions of cytosolic Bcl-2-associated x (Bax), Cyt-c, cleaved caspase-3, calmodulin (CaM), Ca2+/CaM-dependent protein kinases II (CaMKII) and phosphorylated CaMKII were increased significantly in cypermethrin-exposed TM4 cells. Cypermethrin decreased mitochondrial membrane potential significantly. Then, Bcl-2 family and Ca2+/CaM/CaMKII pathway participate in cypermethrin-induced homeostasis. Ca2+ overload activates mitochondrial pathway by increasing permeability of mitochondrial membrane and decreasing mitochondrial membrane potential. We suggest cypermethrin induces Sertoli cell apoptosis involving mitochondrial pathway associated with Ca2+ regulated by Bcl-2 family and Ca2+/CaM/CaMKII pathway. The study provides a new insight into mechanisms involved in cypermethrin-induced male reproductive toxicology.
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- 2021
17. BMP4 Regulates EMT to be Involved in non-Syndromic Cleft lip With or Without Palate
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Jia-Wei Hong, Yue Yu, Lu-Shan Wang, Zheng Li, Rui Zhang, Qi Wang, Zhen Ding, Jin-Peng Zhang, Mei-Rong Zhang, and Li-Chun Xu
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Otorhinolaryngology ,Oral Surgery - Abstract
Objective In the previous study, we identified bone morphogenetic protein 4 (BMP4) responsible for non-syndromic cleft lip with or without cleft palate (NSCL/P). We aimed to elucidate the effects and mechanisms of BMP4 on epithelial–mesenchymal transition (EMT) through Smad1 signaling pathway to be involved in NSCL/P. Methods The human oral epidermoid carcinoma cells (KBs) were transfected with plasmids or small interfering RNA (siRNA) to build the models. The migration of the cells was evaluated by transwell assay. Western blotting and quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) were used to detect the expressions of BMP4, E-cadherin, N-cadherin, EMT-related transcription factors snal1 and snal2, matrix metalloproteinase 2 (MMP2), MMP9, Smad1, and phosphorylated Smad1. Results In the overexpression group, the migration number of cells was increased significantly. The protein expression of E-cadherin was decreased significantly, while the protein expression level of the N-cadherin was increased significantly. The protein and mRNA expressions of MMP2, MMP9, snal1, and snal2 were significantly higher. The expression level of Smad1 was not significantly changed, while the phosphorylation of Smad1 was significantly increased. In the BMP4-siRNA group, the migrating number cells was significantly decreased. The protein expression of E-cadherin was increased significantly, while the expression of N-cadherin was significantly decreased. The protein and mRNA expressions of MMP2, MMP9, snal1, and snal2 were significantly lower than that of the control group. The expressions of Smad1 and phosphorylation of Smad1 were not significantly changed. Conclusions BMP4 enhances cell migration and promotes cell EMT through Smad1 signaling pathway. Abnormal BMP4 mediates migration and EMT through other relevant signaling pathways resulting in NSCL/P. The study provides new insight into the mechanisms of NSCL/P associated with BMP4.n
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- 2022
18. Cooperative sliding-mode guidance law for active protection
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Wen-Hua, Hua, primary and Jin-Peng, Zhang, additional
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- 2022
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19. Millennial-Scale Organic Carbon Storage in Coastal Sediments Regulated by the Changes of Relative Sea Level in the Pearl River Estuary, South China
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Qing Zhu, Fang Chen, Shao-Hua Yu, Yang Zhou, Hua-Yang Gan, Jin-Peng Zhang, Cong Wu, Jin-Lian Wang, Chang Zhuang, and Jiang-Hai Wang
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
20. Mitogen-activated protein kinase TaMPK3 suppresses ABA response by destabilising TaPYL4 receptor in wheat
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Ying Liu, Tai‐Fei Yu, Yi‐Tong Li, Lei Zheng, Zhi‐Wei Lu, Yong‐Bin Zhou, Jun Chen, Ming Chen, Jin‐Peng Zhang, Guo‐Zhong Sun, Xin‐You Cao, Yong‐Wei Liu, You‐Zhi Ma, and Zhao‐Shi Xu
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Physiology ,Gene Expression Regulation, Plant ,Seedlings ,Stress, Physiological ,Plant Science ,Mitogen-Activated Protein Kinases ,Carrier Proteins ,Plants, Genetically Modified ,Abscisic Acid ,Droughts ,Plant Proteins - Abstract
Abscisic acid (ABA) receptors are considered as the targeted manipulation of ABA sensitivity and water productivity in plants. Regulation of their stability or activity will directly affect ABA signalling. Mitogen-activated protein kinase (MAPK) cascades link multiple environmental and plant developmental cues. However, the molecular mechanism of ABA signalling and MAPK cascade interaction remains largely elusive. TaMPK3 overexpression decreases drought tolerance and wheat sensitivity to ABA, significantly weakening ABA's inhibitory effects on growth. Under drought stress, overexpression lines show lower survival rates, shoot fresh weight and proline content, but higher malondialdehyde levels at seedling stage, as well as decreased grain width and 1000 grain weight in both glasshouse and field conditions at the adult stage. TaMPK3-RNAi increases drought tolerance. TaMPK3 interaction with TaPYL4 leads to decreased TaPYL4 levels by promoting its ubiquitin-mediated degradation, whereas ABA treatment diminishes TaMPK3-TaPYL interactions. In addition, the expression of ABA signalling proteins is impaired in TaMPK3-overexpressing wheat plants under ABA treatment. The MPK3-PYL interaction module was found to be conserved across monocots and dicots. Our results suggest that the MPK3-PYL module could serve as a negative regulatory mechanism for balancing appropriate drought stress response with normal plant growth signalling in wheat.
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- 2021
21. Temperature field evolution and heat transfer during continual local induction cladding
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Si-yu Liu, Jin-peng Zhang, Jun Zhan, and Xunpeng Qin
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0209 industrial biotechnology ,Heat-affected zone ,Materials science ,Metals and Alloys ,General Engineering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Microstructure ,Inductor ,Cladding (fiber optics) ,Temperature measurement ,Grain size ,Finite element method ,020901 industrial engineering & automation ,Heat transfer ,Composite material ,0210 nano-technology - Abstract
The evolution of temperature field of the continual motion induction cladding and the depth of heat affected zone are studied in this study. A three-dimensional finite element model for the point type continual induction cladding is established to investigate temperature distributions of fixed and motion induction cladding modes. The novel inductor is designed for cladding of curved surfaces. The modeling reliability is verified by the temperature measurements. The influence of process parameters on the maximum temperature and the generation and transfer of heat are studied. Quantitative calculation is performed to its melting rate to verify the temperature distribution and microstructures. The results show that a good metallurgical bond can be formed between the cladding layer and substrate. The melting rate gradually falls from the top of the cladding layer to the substrate, and the grain size in the substrate gradually rises. The heat affected zone is relatively small compared to integral heating.
- Published
- 2020
22. Chinese-Khmer Parallel fragments Extraction from Comparable Corpus Based on Dirichlet Process
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Xin Yan, Qing Xie, Ning Shan, Jin Peng Zhang, Yu Nuo, and Feng Zhou
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Topic model ,Matching (graph theory) ,Computer science ,business.industry ,Computer Science::Computation and Language (Computational Linguistics and Natural Language and Speech Processing) ,020206 networking & telecommunications ,02 engineering and technology ,Filter (higher-order function) ,Poisson distribution ,computer.software_genre ,Field (computer science) ,Dirichlet process ,symbols.namesake ,Fragment (logic) ,0202 electrical engineering, electronic engineering, information engineering ,symbols ,General Earth and Planetary Sciences ,020201 artificial intelligence & image processing ,Artificial intelligence ,business ,computer ,Natural language processing ,General Environmental Science ,Gibbs sampling - Abstract
Aiming at the problems existing in the Chinese-Khmer parallel corpus, such as single field, small scale, and poor timeliness, a method of Chinese-Khmer parallel fragment extraction from comparable corpus based on Dirichlet process is proposed. The method firstly obtains the topic distribution of bilingual comparable corpus through bilingual topic model, then uses Poisson distribution to randomly divide the bilingual texts, sets up a threshold to initially filter parallel fragment of comparable corpus, and then obtains the matching probability between parallel fragments by Dirichlet process. Obtaining the final parallel fragments by Gibbs sampling. From the comparison experiments, the method of parallel fragment extraction from bilingual comparable corpus based on Dirichlet process can obtain higher quality parallel fragments without providing any parallel data, which is more suitable for languages where bilingual resources are low.
- Published
- 2020
23. Deep learning for inversion of significant wave height based on actual sea surface backscattering coefficient model
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Zhensen Wu, Tan Qu, Tao Wu, Yunhua Cao, Jin-Peng Zhang, and Jiaji Wu
- Subjects
Backscatter ,Computer Networks and Communications ,Computer science ,020207 software engineering ,Inversion (meteorology) ,02 engineering and technology ,Geodesy ,Electromagnetic radiation ,Swell ,Root mean square ,Hardware and Architecture ,Wind wave ,0202 electrical engineering, electronic engineering, information engineering ,Media Technology ,Significant wave height ,Software - Abstract
Ocean waves are complex systems with the contributions of wind waves and swells. The study on interaction mechanism between electromagnetic wave and actual sea surface is of significant importance in ocean remote sensing and engineering application, which is also helpful in the prediction and inversion of wave information. In this paper, an efficient model for estimating backscattering coefficient is built, considering the characteristics of the wind-wave regime based on the inverse wave age. The backscattering coefficient results have been verified by comparing with the data collected in Lingshan Island during the period of October and November 2014 at low grazing angles and the Ku-band measurements at moderate grazing angles. The results indicate perfect agreement (within about 2 dB) with field data. Deep learning is an excellent method that can be used not only for classification but also for inversion and fitting of non-linear functions. In order to simulate the application of actual radar detection and inversion technology, the inversion of significant wave height from actual sea surface backscattering coefficients train data sets has been performed by using deep learning technology. The accuracy of 99.01% has been achieved under the condition of three hidden layers and iterating 100 times. The root mean square errors of the test data sets are less than 0.10, which indicates that deep learning is available in the inversion of significant wave height.
- Published
- 2019
24. Application of Benzofuran-Derived Azadienes as Two-Carbon Building Blocks in Annulations: Chemo- and Diastereoselective Construction of Spiro-Benzofuran Scaffolds
- Author
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Feng-Tao Sheng, Ye-Xin Wang, Feng Shi, Wei Tan, Yi-Nan Lu, Jin-Peng Zhang, and Lin-Lin Xu
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Annulation ,010405 organic chemistry ,Organic Chemistry ,chemistry.chemical_element ,010402 general chemistry ,01 natural sciences ,Combinatorial chemistry ,Catalysis ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Chemoselectivity ,Benzofuran ,Carbon - Abstract
A base-promoted (2+4) annulation of benzofuran-derived azadienes with para-quinone methide derivatives has been established, which afforded spiro-benzofuran derivatives in generally high yields (57–97%) and with good diastereoselectivities (85:15 to >95:5 dr). This reaction not only represents the first application of benzofuran-derived azadienes as two-carbon building blocks in annulations, but also provides an efficient protocol for the construction of spiro-benzofuran scaffolds with chemoselectivity and high diastereoselectivity. This approach will enrich the chemistry of benzofuran-derived azadiene-involved reactions.
- Published
- 2020
25. Effects of small interfering RNA-mediated silencing of susceptibility genes of non-syndromic cleft lip with or without cleft palate on cell proliferation and migration
- Author
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Jin-Peng Zhang, Meng-Xue Li, Lu-Shan Wang, Qi Wang, Mei-Rong Zhang, Yue Yu, Rui Zhang, Zhen Ding, Zheng Li, and Li-Chun Xu
- Subjects
Small interfering RNA ,Genotype ,Cleft Lip ,Vesicular Transport Proteins ,Kinesins ,beta-Lactamases ,Mitochondrial Proteins ,03 medical and health sciences ,0302 clinical medicine ,RNA interference ,Cell Movement ,030225 pediatrics ,Medicine ,Gene silencing ,Humans ,Genetic Predisposition to Disease ,Gene Silencing ,RNA, Small Interfering ,030223 otorhinolaryngology ,Gene ,Cells, Cultured ,Cell Proliferation ,PDGFC ,Fibroblast growth factor receptor 2 ,Cell growth ,business.industry ,Membrane Proteins ,Cell migration ,General Medicine ,Molecular biology ,Cleft Palate ,Otorhinolaryngology ,Pediatrics, Perinatology and Child Health ,ATP-Binding Cassette Transporters ,business - Abstract
Background Non-syndrome cleft lip with or without cleft palate (NSCL/P) is the most common congenital defect with a complex etiology involving both genetic and environmental factors. Our previous research has identified susceptibility genes of NSCL/P using whole-exome sequencing. The study was to determine the effects of small interfering RNA (siRNA)-mediated silencing of genes on cell proliferation and migration to confirm the roles of the genes in NSCL/P. Methods We silenced the genes by RNA interference (RNAi) with siRNA in human oral keratinocyte (HOK). We used the Cell Counting Kit-8 (CCK8) assay to determine cell proliferation and the wound healing assay to determine cell migration. Results Migration of HOK was inhibited by RNAi-induced silencing of adenosine triphosphate binding cassette transporter A4 (ABCA4), erythropoietin produces hepatocyte A receptor 3 (EPHA3), alpha-parvin (PARVA), and platelet-derived growth factor C (PDGFC). The change of proliferation was not found. Treated with siRNA-mediated silencing of type IV collagen (COL4A2), eukaryotic translation initiation factor 2B subunit (EIF2B3), fibroblast growth factor receptor 2 (FGFR2), kinesin family member 20B (KIF20B), β-lactamase serine-like protein (LACTB), SEC16 homolog A (SEC16A) and thyroid adenoma target gene (THADA) had no effects on cell proliferation and migration of HOK. Conclusions We suggest mutations of the four susceptibility genes ABCA4, EPHA3, PARVA and PDGFC are involved in NSCL/P through inhibiting cell migration. The study provides new candidates for future study of NSCL/P.
- Published
- 2020
26. Application of PSO-GA&CGA in Sea-Clutter Doppler Spectrum Modeling
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Ting-Ting Ji, Shu-Bin Zhang, Wen-Yuan Wang, and Jin-Peng Zhang
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020209 energy ,Computer Science::Neural and Evolutionary Computation ,Particle swarm optimization ,02 engineering and technology ,01 natural sciences ,Field (computer science) ,symbols.namesake ,Search algorithm ,0103 physical sciences ,Genetic algorithm ,0202 electrical engineering, electronic engineering, information engineering ,Key (cryptography) ,symbols ,Clutter ,010306 general physics ,Doppler effect ,Algorithm ,Hamming code - Abstract
In the field of sea clutter research, the key of sea targets recognition and detection is that accuracy estimated parameters of the Doppler Spectrum modeling through the optimal solution search algorithm. Our work provides two ways to improve heuristic search algorithm. One is CGA(Continuesmutation Genetic Algorithm) that cloud prevent Hamming Cliff, which is considered as GA’s inherent issue. The other is that the PSO-GA (Particle Swarm Optimization and Genetic Algorithm) and PSO-CGA (Particle Swarm Optimization and Continuesmutation Genetic Algorithm) obtained by combining algorithms, which can take advantages of the original methods and increase the individuals utilization rate. Both these method can reduce the error of the obtained results without increase solving time. In addition, we also proposes a new evaluation method of the heuristic search algorithm to efficiency measure algorithms.
- Published
- 2020
27. Identification of key genes in cleft lip with or without cleft palate regulated by miR-199a-5p
- Author
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Xing Ge, Li-Chun Xu, Jin-Peng Zhang, Jia-Wei Hong, Zhen Ding, Meng-Xue Li, Jun-Yu Shen, Qiao-Mei Shi, Gang Chen, Heng-Xue Wang, and Qi Wang
- Subjects
Genetic Markers ,0301 basic medicine ,Hippo signaling pathway ,TGF alpha ,MiRTarBase ,business.industry ,Cleft Lip ,Computational Biology ,General Medicine ,Computational biology ,Transforming Growth Factor alpha ,MiRBase ,Cleft Palate ,Focal adhesion ,MicroRNAs ,03 medical and health sciences ,030104 developmental biology ,Otorhinolaryngology ,Transcription (biology) ,Pediatrics, Perinatology and Child Health ,microRNA ,Humans ,Medicine ,business ,Gene - Abstract
Background Cleft lip with or without cleft palate (CL/P) is one of the most common congenital defects, which etiology involves both genetic and environmental factors. Previous studies have shown that miR-199a-5p may mediate the occurrence of CL/P. However, the key target genes regulated by miR-199a-5p are not clear. In this study, we employed a systematic bioinformatics analysis of target genes regulated by miR-199a-5p which may be involved in CL/P. Methods The miRBase, Human miRNA tissue atlas, miRecords, miRpathDB, miRWalk, miRTarBase, DIANA-TarBase (v7.0), Literature search, DAVID software, Cytoscape plugin ClueGO + Cluepedia app, MalaCards, TargetScanhuman7.1, Venny 2.1, STRING and GEO databases were comprehensive employed to identify the key genes regulated by miR-199a-5p associated with CL/P. Results Total 429 experimentally validated target genes were obtained from five miRNAs related databases. Expressions of miR-199a-5p and its experimentally validated target genes were elevated in bone, brain and skin. KEGG pathway analysis revealed that the target genes were enriched in focal adhesion, microRNAs in cancer and hippo signaling pathway. Biological process categorization revealed that significant portions of the target genes were grouped as transcription, DNA-templated. Total eight intersection genes were identified by using MalaCards and TargetScanhuman7.1. The target gene transforming growth factor alpha (TGFA) of miR-199a-5p involved in CL/P is screened and verified. Conclusion MiR-199a-5p may mediate CL/P by regulating key target gene TGFA. The study may contribute to a better understanding of the etiology of CL/P.
- Published
- 2018
28. Hantavirus Gc induces long-term immune protection via LAMP-targeting DNA vaccine strategy
- Author
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Zichao Li, Jin-Peng Zhang, Yuchen Lu, Zhen-Hua Lu, Lianhe Zheng, Lin-Feng Cheng, Fanglin Zhang, Dongbo Jiang, Yun Li, Guan-Wen Zhang, Kun Yang, and Zi-Xin Zhang
- Subjects
0301 basic medicine ,Orthohantavirus ,Cellular immunity ,Hantavirus Infections ,Recombinant Fusion Proteins ,Antigen presentation ,Antibodies, Viral ,Major histocompatibility complex ,Epitope ,Cell Line ,DNA vaccination ,Mice ,Viral Proteins ,03 medical and health sciences ,Immune system ,Antigen ,Neutralization Tests ,Virology ,Vaccines, DNA ,Animals ,Humans ,Pharmacology ,Immunity, Cellular ,biology ,Lysosome-Associated Membrane Glycoproteins ,Antibodies, Neutralizing ,Disease Models, Animal ,030104 developmental biology ,Inactivated vaccine ,biology.protein ,Female ,Immunologic Memory ,Epitope Mapping ,Plasmids - Abstract
Hemorrhagic fever with renal syndrome (HFRS) occurs widely throughout Eurasia. Unfortunately, there is no effective treatment, and prophylaxis remains the best option against the major pathogenic agent, hantaan virus (HTNV), which is an Old World hantavirus. However, the absence of cellular immune responses and immunological memory hampers acceptance of the current inactivated HFRS vaccine. Previous studies revealed that a lysosome-associated membrane protein 1 (LAMP1)-targeting strategy involving a DNA vaccine based on the HTNV glycoprotein Gn successfully conferred long-term immunity, and indicated that further research on Gc, another HTNV antigen, was warranted. Plasmids encoding Gc and lysosome-targeted Gc, designated pVAX-Gc and pVAX-LAMP/Gc, respectively, were constructed. Proteins of interest were identified by fluorescence microscopy following cell line transfection. Five groups of 20 female BALB/c mice were subjected to the following inoculations: inactivated HTNV vaccine, pVAX-LAMP/Gc, pVAX-Gc, and, as the negative controls, pVAX-LAMP or the blank vector pVAX1. Humoral and cellular immunity were assessed by enzyme-linked immunosorbent assays (ELISAs) and 15-mer peptide enzyme-linked immunospot (ELISpot) epitope mapping assays. Repeated immunization with pVAX-LAMP/Gc enhanced adaptive immune responses, as demonstrated by the specific and neutralizing antibody titers and increased IFN-γ production. The inactivated vaccine induced a comparable humoral reaction, but the negative controls only elicited insignificant responses. Using a mouse model of HTNV challenge, the in vivo protection conferred by the inactivated vaccine and Gc-based constructs (with/without LAMP recombination) was confirmed. Evidence of pan-epitope reactions highlighted the long-term cellular response to the LAMP-targeting strategy, and histological observations indicated the safety of the LAMP-targeting vaccines. The long-term protective immune responses induced by pVAX-LAMP/Gc may be due to the advantage afforded by lysosomal targeting after exogenous antigen processing initiation and major histocompatibility complex (MHC) class II antigen presentation trafficking. MHC II-restricted antigen recognition effectively primes HTNV-specific CD4+ T-cells, leading to the promotion of significant immune responses and immunological memory. An epitope-spreading phenomenon was observed, which mirrors the previous result from the Gn study, in which the dominant IFN-γ-responsive hot-spot epitopes were shared between HLA-II and H2d. Importantly, the pan-epitope reaction to Gc indicated that Gc should be with potential for use in further hantavirus DNA vaccine investigations.
- Published
- 2018
29. Study on Micro-expansive Concrete-Filled Steel Tube Support for Controlling the Stability of Surrounding Rock in Deep Roadway
- Author
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Jin-peng Zhang and Li-min Liu
- Subjects
business.industry ,Soil Science ,020101 civil engineering ,Geology ,02 engineering and technology ,Structural engineering ,Deformation (meteorology) ,Geotechnical Engineering and Engineering Geology ,0201 civil engineering ,Water reducer ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Architecture ,Strength testing ,Steel tube ,business ,Expansive - Abstract
The overall supporting capacity of ordinary concrete-filled steel tube support (CFSTS) can be decreased due to the self-shrinkage of concrete and the cracks of hardened concrete. The micro-expansive CFSTS is put forward in this paper. Firstly, the optimum proportion of micro-expansion concrete was determined by concrete strength testing. Then, the deformation of micro-expansion CFSTS and ordinary CFSTS under the same load w studied by numerical simulation. Finally, the micro-expansion CFSTS and ordinary CFSTS were applied to the roadway respectively. The results show: The expansive agent content and water reducer content is 11 and 0.5% in the optimum proportion of micro-expansive concrete, respectively. Under the same load, the deformation of the ordinary CFSTS was much larger than that of the micro-expansion CFSTS. The deformations of roof–floor and two sides of roadway supported by the micro-expansion CFSTS were obviously smaller than that of roadway supported by the ordinary CFSTS. The micro-expansion CFSTS has stronger supporting capacity than ordinary CFSTS.
- Published
- 2018
30. Tunable shape recovery progress of thermoplastic polyurethane by solvents
- Author
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Kou Bo, Ziqi Wu, Lu Tianxuan, Wang Changchun, Hang Zusheng, Jin-Peng Zhang, and Xuejuan Zhao
- Subjects
chemistry.chemical_classification ,Materials science ,Ether ,02 engineering and technology ,Molding (process) ,Polymer ,Shape-memory alloy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Annealing (glass) ,chemistry.chemical_compound ,Hildebrand solubility parameter ,Thermoplastic polyurethane ,chemistry ,Materials Chemistry ,Acetone ,Composite material ,0210 nano-technology - Abstract
Purpose This study aims to present that the chemo-responsive shape recovery of thermoplastic polyurethane (TPU) is tunable by solvents with different solubility parameters, and it is generic for chemo-responsive shape-memory polymer and its composites. Design/methodology/approach Two kinds of commercial TPU samples with different thicknesses were prepared by panel vulcanizer and injection molding (an industrial manner) to investigate their chemo-responsive shape memory properties in acetic ether and acetone. Findings Results showed that all of TPU films with different thicknesses can fully recover their original shapes weather they recover in acetic ether or acetone. But the recovery time of TPU films in acetone is greatly reduced, especially for the twisting samples. The residual strains of recovery TPU samples after extension reduce obviously. Research limitations/implications The great decrement of recovery time is related to two factors. One is due to the bigger solubility parameter of acetone with higher dipole moment compared with those of acetic ether, and the other is the remained internal stress of TPU films after preparation. The internal stress is identified to have an effect on the shape-memory properties by comparing the recovery process of samples with/without annealing. The reduced residual strains of recovery TPU samples after extension is due to the increasing mobility of polymer segments after molecules of acetic ether penetrates into the polymeric chains. Originality/value This is a universal strategy to control the recovery process of shape-memory materials or composites. The underlying mechanism is generic and should be applicable to chemo-responsive shape-memory polymers or their composites.
- Published
- 2018
31. Cypermethrin inhibits interleukin-6-induced androgen receptor transactivation through signal transducer and activator of transcription 3
- Author
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Qi Wang, Ji-Long Zhou, Li-Chun Xu, Gang Chen, Mei-Rong Zhang, Xing Ge, Hui Wang, Heng-Xue Wang, Zhen Ding, Qiang Ju, Jin-Peng Zhang, Qiao-Mei Shi, and Meng-Xue Li
- Subjects
STAT3 Transcription Factor ,Transcriptional Activation ,0301 basic medicine ,Insecticides ,Health, Toxicology and Mutagenesis ,Toxicology ,Cell Line ,Cypermethrin ,03 medical and health sciences ,Transactivation ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,Pyrethrins ,medicine ,Animals ,Humans ,Phosphorylation ,Interleukin 6 ,STAT3 ,biology ,medicine.diagnostic_test ,Interleukin-6 ,Molecular biology ,Androgen receptor ,030104 developmental biology ,chemistry ,Receptors, Androgen ,030220 oncology & carcinogenesis ,STAT protein ,biology.protein - Abstract
The insecticide cypermethrin has been considered as an endocrine-disrupting chemicals (EDCs) with anti-androgenic activity by interfering with interleukin-6 (IL-6) – induced ligand-independent AR signaling. The purpose of this study was to clarify whether the signal transducer and activator of transcription 3 (STAT3) was involved in the antagonism effect of cypermethrin. In this study, the Western blot was to test the level of STAT3 phosphorylation and the mammalian two-hybrid assay was developed to assess the AR–STAT3 interaction. The date showed that IL-6 increased the phosphorylation level of STAT3 and enhanced the AR–STAT3 interaction. Cypermethrin did not affect the phosphorylation level of STAT3 induced by IL-6, while suppressed the AR-STAT3 interaction induced by IL-6 significantly at the concentration of 10−5 M (p
- Published
- 2017
32. Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice
- Author
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Jin-Peng Zhang, Dongbo Jiang, Shao-Bo Xiao, Li-Juan Sun, Yuanjie Sun, Shuya Yang, Fanglin Zhang, Lin-Feng Cheng, Jing Wang, and Kun Yang
- Subjects
0301 basic medicine ,Enzyme-Linked Immunospot Assay ,Orthohantavirus ,Hantavirus Infections ,030106 microbiology ,DNA, Recombinant ,Biology ,Antibodies, Viral ,Epitope ,DNA vaccination ,law.invention ,Mice ,Viral Proteins ,03 medical and health sciences ,Immune system ,Antigen ,law ,Virology ,Vaccines, DNA ,Animals ,Hantavirus ,Pharmacology ,Mice, Inbred BALB C ,Membrane Glycoproteins ,ELISPOT ,Lysosome-Associated Membrane Glycoproteins ,Viral Vaccines ,DNA ,Antibodies, Neutralizing ,030104 developmental biology ,Immunology ,Recombinant DNA ,biology.protein ,Antibody ,Immunologic Memory - Abstract
Background Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring long-term evaluations. Methods & results Immune responses of Balb/c mice were assessed by specific and neutralizing antibodies, interferon-γ ELISpot assay, and cytotoxic T-lymphocyte cytotoxicity assay. HTNV-challenge assay identified long-term protection. Safety was confirmed by histological and behavioral analysis. Epitope-spreading phenomenon was noted, revealing two sets of dominant T-cell epitopes cross-species. Conclusion pVAX-LAMP/Gn established memory responses within a long-term protection. Lysosome-targeted strategy showed promise on Gn-based DNA vaccine and further investigations are warranted in other immunogenic Hantaviral antigens.
- Published
- 2017
33. Inhibition effect of cypermethrin mediated by co-regulators SRC-1 and SMRT in interleukin-6-induced androgen receptor activation
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Jin-Peng Zhang, Mei-Rong Zhang, Zhen Ding, Li-Chun Xu, Xiao-Long Zhou, Ji-Long Zhou, Xing Ge, Hui Wang, Qiang Ju, Qiao-Mei Shi, Hong-Min Yu, Chen Pan, and Qi Wang
- Subjects
Male ,0301 basic medicine ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Pharmacology ,Biology ,Cell Line ,Cypermethrin ,03 medical and health sciences ,chemistry.chemical_compound ,Nuclear Receptor Coactivator 1 ,0302 clinical medicine ,Cell Line, Tumor ,Two-Hybrid System Techniques ,Chlorocebus aethiops ,Pyrethrins ,LNCaP ,Androgen Receptor Antagonists ,Animals ,Humans ,Environmental Chemistry ,Nuclear Receptor Co-Repressor 2 ,Receptor ,Thyroid hormone receptor ,Interleukin-6 ,Public Health, Environmental and Occupational Health ,Prostatic Neoplasms ,General Medicine ,General Chemistry ,Pollution ,Nuclear receptor coactivator 1 ,Androgen receptor ,030104 developmental biology ,chemistry ,Receptors, Androgen ,030220 oncology & carcinogenesis ,Antagonism - Abstract
It is hypothesized that the pesticide cypermethrin may induce androgen receptor (AR) antagonism via ligand-independent mechanisms. The Real-Time Cell Analysis (RTCA) iCELLigence system was used to investigate the inhibitory effect of cypermethrin on interleukin-6 (IL-6)-induced ligand-independent LNCaP cell growth. Then, the mammalian two-hybrid assays were applied to clarify whether the mechanism of IL-6-induced AR antagonism of cypermethrin was associated with the interactions of the AR and co-activator steroid receptor co-activator-1 (SRC-1) and co-repressor silencing mediator for retinoid and thyroid hormone receptors (SMRT). Cypermethrin inhibited the LNCaP cell growth induced by IL-6. The interactions of AR-SRC-1 and AR-SMRT mediated by IL-6 were suppressed by cypermethrin. The results indicate that the IL-6-mediated AR antagonism induced by cypermethrin is related to repress the recruitment of co-regulators SRC-1 and SMRT to the AR in a ligand-independent manner. Inhibition of the interactions of AR-SRC-1 and AR-SMRT mediated by IL-6 contributes to the AR antagonism induced by cypermethrin.
- Published
- 2016
34. Inhibitory Effects of Cypermethrin on Interactions of the Androgen Receptor with Coactivators ARA70 and ARA55
- Author
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Zhen, Ding, Jun Yu, Shen, Jia Wei, Hong, Rui, Zhang, Zheng, Li, Qi, Wang, Jin Peng, Zhang, Mei Rong, Zhang, and Li Chun, Xu
- Subjects
Insecticides ,Receptors, Androgen ,Two-Hybrid System Techniques ,Chlorocebus aethiops ,Nuclear Receptor Coactivators ,Pyrethrins ,Androgen Receptor Antagonists ,Intracellular Signaling Peptides and Proteins ,Animals ,Androgen Antagonists ,LIM Domain Proteins ,Cell Line - Abstract
The purpose of this study was to investigate the anti-androgenic mechanism of cypermethrin involving coactivators.Mammalian two-hybrid assays were performed to study the effects of cypermethrin on interactions of the androgen receptor (AR) with the coactivators androgen receptor-associated protein 70 (ARA70) and androgen receptor-associated protein 55 (ARA55).The results showed that AR-ARA70 and AR-ARA55 interactions were remarkably enhanced by dihydrotestosterone (DHT,The study indicates that cypermethrin exhibits inhibitory effects on AR transcription associated with repression of AR-ARA70 and AR-ARA55 interactions in a ligand-dependent manner. The data show novel anti-androgenic mechanisms of cypermethrin that contribute to male reproductive toxicology.
- Published
- 2019
35. The anti-androgenic effects of cypermethrin mediated by non-classical testosterone pathway activation of mitogen-activated protein kinase cascade in mouse Sertoli cells
- Author
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Mei-Rong Zhang, Jia-Wei Hong, Qi Wang, Gang Chen, Li-Chun Xu, Zhen Ding, Heng-Xue Wang, Meng-Xue Li, Jin-Peng Zhang, Jing Li, Zheng Li, Jun-Yu Shen, and Rui Zhang
- Subjects
MAPK/ERK pathway ,Male ,MAP Kinase Signaling System ,Health, Toxicology and Mutagenesis ,0211 other engineering and technologies ,02 engineering and technology ,010501 environmental sciences ,urologic and male genital diseases ,CREB ,01 natural sciences ,Mice ,Epidermal growth factor ,Pyrethrins ,medicine ,Animals ,Inhibins ,Testosterone ,Phosphorylation ,Protein kinase A ,Cyclic AMP Response Element-Binding Protein ,Androgen-binding protein ,0105 earth and related environmental sciences ,Cell Proliferation ,021110 strategic, defence & security studies ,Sertoli Cells ,biology ,Chemistry ,Public Health, Environmental and Occupational Health ,Androgen Antagonists ,General Medicine ,Sertoli cell ,Pollution ,Cell biology ,ErbB Receptors ,medicine.anatomical_structure ,Gene Expression Regulation ,biology.protein ,Androgens ,Signal transduction ,Mitogen-Activated Protein Kinases ,Proto-oncogene tyrosine-protein kinase Src ,Signal Transduction - Abstract
Previous studies have demonstrated that the anti-androgenic effects of cypermethrin (CYP) are associated with testosterone (T) - related signaling pathway. This study was to investigate the effects of CYP on mouse Sertoli cells (TM4) and clarify whether the mechanisms were mediated by non-classical T signaling pathway activating mitogen-activated protein kinase (MAPK) cascade. The Cell Counting Kit 8 (CCK8) and Real-Time Cell Analysis iCELLigence (RTCA-iCELLigence) system were performed to detect the effects of 10 μM, 20 μM, 40 μM and 80 μM CYP on the viability and proliferation of TM4. The mammalian two hybrid assay, quantitative Real-Time PCR (qRT-PCR) and western blot were conducted to analyze the key genes and proteins involved in T-mediated MAPK signaling pathway. CYP was found to inhibit the viability and proliferation of TM4. Additionally, CYP disturbed the functions of Sertoli cells by inhibiting inhibin B (INH B) expression and facilitating androgen binding protein (ABP) and transferrin (TF) expression. Moreover, CYP suppressed the interaction of AR and Src kinase and inhibited androgen-mediated phosphorylation of Src, epidermal growth factor receptor (EGFR), extracellular-regulated kinase1/2 (ERK1/2) and transcription factor cAMP response element binding protein (CREB). Furthermore, the androgen-induced mRNA and protein expression of CREB-regulated gene early growth response factor (Egr1) decreased after treated with CYP. It is indicated that CYP inhibits the viability and proliferation of Sertoli cells and non-classical T signaling pathway activation of MAPK cascade is involved in anti-androgenic effect of CYP. This study provides a novel insight into the CYP-induced reproductive toxicity.
- Published
- 2018
36. Parallel computation of Doppler spectrum from dynamic sea surfaces at microwave bands
- Author
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Longxiang Linghu, Jiaji Wu, Jin-Peng Zhang, and Zhensen Wu
- Subjects
Scattering ,Computer science ,Gravitational wave ,0211 other engineering and technologies ,Bragg's law ,02 engineering and technology ,Computational science ,symbols.namesake ,Superposition principle ,symbols ,Doppler effect ,Microwave ,ComputingMethodologies_COMPUTERGRAPHICS ,021101 geological & geomatics engineering ,Data transmission - Abstract
The Doppler spectrum of the electromagnetic (EM) scattering field from the two-dimensional dynamic sea surface is calculated based on the composite scattering model. The two-dimensional dynamic sea surfaces are generally simulated as a superposition of large-scale gravity waves and small-scale capillary ripples. On this basis, the Doppler spectrum of the EM scattering field from the two-dimensional dynamic sea surface can be calculated based on the composite scattering model, which takes both the quasi-specular scattering and Bragg scattering mechanism into account. However, due to the high resolution and real-time dynamic complexity of the dynamic sea surfaces, the calculation of the Doppler spectrum will be computationally expensive and very time-consuming. In this paper, a GPU-based algorithm of Doppler spectrum was proposed by utilizing the Tesla K80 GPUs with diverse CUDA optimization techniques. The GPU-based Doppler spectrum implementation includes five optimization strategies: first, the temporary arrays are utilized to reduce the repeat float-points operations in the loop; then the device memory was effectively exploited to reduce the data transfer time between the CPU and GPU; the fast math compiler option was also utilized to further improve the computation performance of the Doppler spectrum calculation; finally the data transfer time between the device and host memories can be effectively hide by using the asynchronous data transfer (ADT). Compared to the CPU serial program executed on Intel(R) Core(TM) i5-3450 CPU, the GPU-based Doppler spectrum implementation can achieve a significant speedup of1200× .
- Published
- 2018
37. Cypermethrin induces Sertoli cell apoptosis through mitochondrial pathway associated with calcium.
- Author
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Heng-Xue Wang, Rui Zhang, Zheng Li, Lu-Shan Wang, Yue Yu, Qi Wang, Zhen Ding, Jin-Peng Zhang, Mei-Rong Zhang, and Li-Chun Xu
- Subjects
ENDOCRINE disruptors ,CYPERMETHRIN ,SERTOLI cells ,MITOCHONDRIAL pathology ,CALCIUM - Abstract
Cypermethrin, one kind of pyrethroid pesticides, has been shown to act as endocrine-disrupting chemicals (EDCs). The purpose of this study was to explore the roles of Sertoli cell apoptosis through mitochondrial pathway associated with calcium (Ca
2+ ) in cypermethrin-induced male reproductive toxicology. The mouse Sertoli cells TM4 were cultured with 0 μM, 10 μM, 20 μM, 40 μM and 80 μM of cypermethrin. We used flow cytometry, Fluo-4 AM, western blot and JC-1 Assay Kit to examine apoptosis, intracellular Ca2+ , expressions of mitochondrial apoptotic pathway-related proteins and mitochondrial membrane potential. We found cypermethrin increased apoptosis rate of TM4 cells significantly and with a significant increase in intracellular Ca2+ concentration. Cypermethrin significantly decreased the protein expressions of cytosolic B-cell lymphoma-2 (Bcl-2) and mitochondrial cytochrome c (Cyt-c). The protein expressions of cytosolic Bcl-2-associated x (Bax), Cyt-c, cleaved caspase-3, calmodulin (CaM), Ca2+ /CaM-dependent protein kinases II (CaMKII) and phosphorylated CaMKII were increased significantly in cypermethrin-exposed TM4 cells. Cypermethrin decreased mitochondrial membrane potential significantly. Then, Bcl-2 family and Ca2+ /CaM/CaMKII pathway participate in cypermethrin-induced homeostasis. Ca2+ overload activates mitochondrial pathway by increasing permeability of mitochondrial membrane and decreasing mitochondrial membrane potential. We suggest cypermethrin induces Sertoli cell apoptosis involving mitochondrial pathway associated with Ca2+ regulated by Bcl-2 family and Ca2+ /CaM/CaMKII pathway. The study provides a new insight into mechanisms involved in cypermethrin-induced male reproductive toxicology. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
38. Sensitive chemiluminescence immunoassay for staphylococcal enterotoxin C1 based on the use of dye-encapsulated mesoporous silica nanoparticles
- Author
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Zhujun Zhang, Jin-Peng Zhang, Xiaohua Li, Chunmei Zhang, Kuocheng Yan, Liang Tao, Kun Yang, Boquan Jin, and Yuanjie Sun
- Subjects
Detection limit ,Chromatography ,02 engineering and technology ,Enterotoxin ,Mesoporous silica ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Oxalate ,0104 chemical sciences ,Analytical Chemistry ,law.invention ,Rhodamine 6G ,Hydrophobic effect ,chemistry.chemical_compound ,chemistry ,law ,Fluorescein ,0210 nano-technology ,Chemiluminescence - Abstract
A chemiluminescent immunoassay for the staphylococcal enterotoxin C1 (SEC1) based on the use of dye-encapsulated mesoporous silica nanoparticles (m-SiNPs) as a label is described. The dyes are retained in the m-SiNPs via strong hydrophobic interactions. The assay comprises the following steps: (a) Microplates coated with antibody against SEC1 are filled with sample upon which the SEC antigen will be bound to the surface; (b) following a washing step, secondary antibody linked to m-SiNPs (that were covalently labeled with rhodamine 6G and fluorescein) were added to form the sandwich complex; (c) after another washing step, bis(2,4,6-trichlorophenyl) oxalate, H2O2 and imidazole are added to generate chemiluminescence whose intensity is proportional to the number of m-SiNPs and thus to the number of antigen (SEC) molecules. It is found that the use of functionalized m-SiNPs strongly amplifies the signal. Enterotoxin SEC1 can be detected by this method in the 0.025 to 2 ng⋅mL‾1 concentration range, the detection limit is 19 pg⋅mL‾1 (at 3σ), and the relative standard deviation (for 11 parallel measurements at a 1 ng⋅mL‾1 level) is 4.6 %. The use of an automated chemiluminescence analyzer further improves detection.
- Published
- 2016
39. Effects and mechanisms of pyrethroids on male reproductive system
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Jia-Wei Hong, Heng-Xue Wang, Jin-Peng Zhang, Zheng Li, Qi Wang, Jun-Yu Shen, Zhen Ding, Rui Zhang, Li-Chun Xu, and Mei-Rong Zhang
- Subjects
Male ,0301 basic medicine ,Insecticides ,Estrogen receptor ,Physiology ,Endocrine Disruptors ,Genitalia, Male ,Biology ,Toxicology ,medicine.disease_cause ,Risk Assessment ,03 medical and health sciences ,0302 clinical medicine ,Pyrethrins ,parasitic diseases ,medicine ,Animals ,Humans ,Male reproductive system ,Reproductive health ,business.industry ,Reproduction ,Androgen receptor ,Reproductive Health ,030104 developmental biology ,Steroid synthesis ,business ,Reproductive toxicity ,030217 neurology & neurosurgery ,Oxidative stress ,Signal Transduction - Abstract
Synthetic pyrethroids are used as insecticides in agriculture and a variety of household applications worldwide. Pyrethroids are widely distributed in all environmental compartments and the general populations are exposed to pyrethroids through various routes. Pyrethroids have been identified as endocrine-disrupting chemicals (EDCs) which are responsible for the male reproductive impairments. The data confirm pyrethroids cause male reproductive damages. The insecticides exert the toxic effects on male reproductive system through various complex mechanisms including antagonizing androgen receptor (AR), inhibiting steroid synthesis, affecting the hypothalamic-pituitary-gonadal (HPG) axis, acting as estrogen receptor (ER) modulators and inducing oxidative stress. The mechanisms of male reproductive toxicity of pyrethroids involve multiple targets and pathways. The review will provide further insight into pyrethroid-induced male reproductive toxicity and mechanisms, which is crucial to preserve male reproductive health.
- Published
- 2020
40. Study on Concrete Carrier Replacing Chain Pillar Between Two Working-faces of the Mining
- Author
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Yang Li, Chen Gao, Li-min Liu, and Jin-peng Zhang
- Subjects
Chain (algebraic topology) ,Computer science ,business.industry ,Pillar ,Structural engineering ,business - Published
- 2018
41. Electroacupuncture combined with iontophoresis of Chinese medicine for lumbar intervertebral disc herniation
- Author
-
Lu-wen Zhu, Qiang Tang, and Jin-peng Zhang
- Subjects
medicine.medical_specialty ,Iontophoresis ,business.industry ,Electroacupuncture ,medicine.medical_treatment ,Traditional Chinese medicine ,Low back pain ,Surgery ,Complementary and alternative medicine ,Intervertebral Disc Displacement ,Anesthesia ,Acupuncture ,medicine ,Clinical efficacy ,medicine.symptom ,business ,Lumbar intervertebral disc - Abstract
Objective To observe the clinical efficacy of electroacupuncture (EA) combined with iontophoresis of Chinese medicine for lumbar intervertebral disc herniation (LIDH).
- Published
- 2015
42. Identification of susceptibility genes in non-syndromic cleft lip with or without cleft palate using whole-exome sequencing
- Author
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Mei-Rong Zhang, Li-Chun Xu, Xiu Peng, Ji-Long Zhou, Hong-Min Yu, Jin-Peng Zhang, Chen Pan, Qin-Rong Wu, Xiao-Long Zhou, Yi-Qun Li, Qi Wang, Yu-Juan Xia, Ya-Peng Liu, and Li-Fang Xu
- Subjects
Nonsynonymous substitution ,Candidate gene ,dbSNP ,Cleft Lip ,Odontología ,Biology ,Polymorphism, Single Nucleotide ,symbols.namesake ,Humans ,Exome ,Genetic Predisposition to Disease ,1000 Genomes Project ,General Dentistry ,Exome sequencing ,Genetics ,Sanger sequencing ,Base Sequence ,Genetic heterogeneity ,Research ,CIENCIAS MÉDICAS [UNESCO] ,Ciencias de la salud ,Cleft Palate ,Otorhinolaryngology ,UNESCO::CIENCIAS MÉDICAS ,symbols ,Surgery ,Oral Surgery - Abstract
Background Non-syndromic cleft lip with or without cleft palate (NSCL/P) is among the most common congenital malformations. The etiology of NSCL/P remains poorly characterized owing to its complex genetic heterogeneity. The objective of this study was to identify genetic variants that increase susceptibility to NSCL/P. Material and Methods Whole-exome sequencing (WES) was performed in 8 fetuses with NSCL/P in China. Bioinformatics analysis was performed using commercially available software. Variants detected by WES were validated by Sanger sequencing. Results By filtering out synonymous variants in exons, we identified average 8575 nonsynonymous single nucleotide variants (SNVs). We subsequently compared the SNVs against public databases including NCBI dbSNP build 135 and 1000 Genomes Project and obtained an average of 203 SNVs. Total 12 reported candidate genes were verified by Sanger sequencing. Sanger sequencing also confirmed 16 novel SNVs shared by two or more samples. Conclusions We have found and confirmed 16 susceptibility genes responsible for NSCL/P, which may play important role in the etiology of NSCL/P. The susceptibility genes identified in this study will not only be useful in revealing the etiology of NSCL/P but also in diagnosis and treatment of the patients with NSCL/P. Key words:Non-syndromic cleft lip with or without cleft palate, whole-exome sequencing, sanger sequencing, susceptibility gene, single nucleotide variants (SNVs).
- Published
- 2015
43. Combustion of crushed and pelletized peanut shells in a pilot-scale fluidized-bed combustor with flue gas recirculation
- Author
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Yuan-Jie Wang, Feng Duan, Jin-Peng Zhang, Chien-Song Chyang, and Jim Tso
- Subjects
Flue gas ,Waste management ,Chemistry ,General Chemical Engineering ,food and beverages ,Energy Engineering and Power Technology ,Flue-gas emissions from fossil-fuel combustion ,Combustion ,Fuel Technology ,Fluidized bed ,Pellet ,Combustor ,Particle ,NOx - Abstract
Peanut shell generated from peanut harvesting has a great potential to be converted into an energy resource in developing countries such as China, Vietnam, and Indonesia. The main objective of this study is to investigate the combustion characteristics of peanut shell in a pilot-scale vortexing fluidized-bed combustor with flue gas recirculation. Crushed and pelletized peanut shells are used as the feeding materials. The effects of in-bed stoichiometric oxygen ratio (Sb), particle sizes, and particle shapes on the combustion characteristics as well as pollutant emissions are investigated. Results show that similar temperature profiles are observed in all cases. CO emissions from the combustion of both fuels are all less than 70 ppm. CO emission decreases, while NOx emissions increase with particle sizes. CO and NOx emissions of pellet combustion show an inverse trend from that of crushed shell combustion at different in-bed stoichiometric oxygen ratios.
- Published
- 2014
44. Anti-androgenic mechanisms of Bisphenol A involve androgen receptor signaling pathway
- Author
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Meng-Xue Li, Xing Ge, Qiao-Mei Shi, Mei-Rong Zhang, Qiang Ju, Heng-Xue Wang, Qi Wang, Jin-Peng Zhang, Hui Wang, Gang Chen, Zhen Ding, and Li-Chun Xu
- Subjects
0301 basic medicine ,Male ,endocrine system ,medicine.medical_specialty ,Androgen receptor signaling pathway ,Sertoli cell proliferation ,010501 environmental sciences ,Biology ,Endocrine Disruptors ,Toxicology ,Transfection ,01 natural sciences ,Cell Line ,03 medical and health sciences ,Mice ,Nuclear Receptor Coactivator 1 ,Phenols ,Internal medicine ,medicine ,Animals ,Nuclear Receptor Co-Repressor 1 ,Nuclear Receptor Co-Repressor 2 ,Benzhydryl Compounds ,Receptor ,0105 earth and related environmental sciences ,Cell Proliferation ,Thyroid hormone receptor ,Sertoli Cells ,Dose-Response Relationship, Drug ,urogenital system ,Haplorhini ,Sertoli cell ,Cell biology ,Androgen receptor ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Nuclear receptor ,Receptors, Androgen ,Signal transduction ,hormones, hormone substitutes, and hormone antagonists ,Protein Binding ,Signal Transduction - Abstract
We have shown Bisphenol A (BPA) acts as an androgen receptor (AR) antagonist in the previous study. However, the mechanisms underlying anti-androgenic effects of BPA remain unclear. The objective of this study was to explore whether the AR signaling was involved in AR antagonism of BPA. The Cell Counting Kit-8 (CCK-8) assay and Real-Time Cell Analysis (RTCA) iCELLigence system were applied to analyze the mouse Sertoli cell TM4 proliferation. The mammalian two-hybrid assays were performed to investigate the effects of BPA on the AR amino- and carboxyl-terminal regions (N/C) interaction and the interactions of the AR with steroid receptor coactivator-1 (SRC-1), co-repressors including silencing mediator for thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (NCoR). BPA exposure resulted in decreased TM4 cell proliferation. BPA inhibited the AR N/C interaction significantly. Furthermore, BPA enhanced the interactions of AR-SMRT and AR-NCoR significantly. In conclusion, these data suggest BPA inhibits Sertoli cell proliferation due to its anti-androgenic actions. The mechanisms responsible for AR antagonism of BPA involve inhibiting the AR N/C interaction and enhancing the interactions of AR-SMRT and AR-NCoR. The data uncover novel anti-androgenic mechanisms by which BPA antagonizes AR signaling, contributing to Sertoli cell proliferation suppression and male reproductive toxicology.
- Published
- 2017
45. The co-regulators SRC-1 and SMRT are involved in interleukin-6-induced androgen receptor activation
- Author
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Ji-Long Zhou, Jin-Peng Zhang, Mei-Rong Zhang, Qiang Ju, Zhen Ding, Li-Chun Xu, Xiao-Long Zhou, Qiao-Mei Shi, Hui Wang, Xing Ge, Qi Wang, and Hong-Min Yu
- Subjects
0301 basic medicine ,Male ,Clinical Biochemistry ,Immunology ,03 medical and health sciences ,0302 clinical medicine ,Nuclear Receptor Coactivator 1 ,Cell Line, Tumor ,LNCaP ,Immunology and Allergy ,Humans ,Nuclear Receptor Co-Repressor 2 ,Receptor ,Thyroid hormone receptor ,Retinoid X receptor alpha ,Chemistry ,Interleukin-6 ,Prostatic Neoplasms ,Retinoid X receptor gamma ,Neoplasm Proteins ,Androgen receptor ,030104 developmental biology ,Hormone receptor ,Receptors, Androgen ,030220 oncology & carcinogenesis ,Cancer research ,Retinoid X receptor beta - Abstract
The androgen receptor (AR) can be stimulated by interleukin-6 (IL-6) in the absence of androgens to induce prostate cancer progression. The purpose of this study was to investigate whether the co-activator steroid receptor coactivator-1 (SRC-1) and co-repressor silencing mediator for retinoid and thyroid hormone receptors (SMRT) are involved in IL-6-induced AR activation. The effects of IL-6 on LNCaP cell proliferation were monitored using real-time cell analysis (RTCA) iCELLigence system. The impacts of IL-6 on the association of the AR with SRC-1 and SMRT were investigated using the mammalian two-hybrid assay. IL-6 increased the proliferation of LNCaP cells with maximal induction at 50 ng/mL. The AR-SRC-1interaction was enhanced by IL-6, with maximal induction at the concentration of 50 ng/mL (P
- Published
- 2017
46. Experimenting Mechanism and Material Optimization of Fe-Ni Foam Metal about Suppressing Gas Explosion and Propagation
- Author
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Hai Bo Sun, Jun Li, Chun Rong Wei, Jin Peng Zhang, and Jian Hua Sun
- Subjects
Shock wave ,Metal ,Materials science ,visual_art ,Gas explosion ,General Engineering ,visual_art.visual_art_medium ,Composite material ,Overpressure ,Adiabatic flame temperature - Abstract
Using self-designed 30cm×30cm gas explosion experiment pipe and data acquisition system, we found Fe-Ni foam metal suppresses better gas explosion. We analyzed mechanism of suppressing gas explosion from material characteristics,. The three-dimensional holing-through structure of foam metal can quench exploding flame and decline exploding shock wave. Foam metal material has dual advantages of material and structure which can suppress transmission of fire and shock wave. Effect on different parameters of Fe-Ni foam metal materials was contrasted from declining overpressure and flame temperature, and suitable Fe-Ni form metal was optimized for engineering application.
- Published
- 2014
47. Modeling signal amplitude of ground-based GPS occultation in marine tropospheric ducts
- Author
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Hui Han, Fang Sun, Zhensen Wu, Hong-guang Wang, and Jin-Peng Zhang
- Subjects
business.industry ,General Physics and Astronomy ,Geodesy ,GPS signals ,Refraction ,Occultation ,Electronic, Optical and Magnetic Materials ,Radio propagation ,symbols.namesake ,Amplitude ,Fourier transform ,Global Positioning System ,symbols ,Electrical and Electronic Engineering ,Reflection coefficient ,business ,Geology - Abstract
The propagation characteristics of Global Positioning System (GPS) signals at very low elevation angles are frequently affected by tropospheric refraction as the satellite rises or sets at the horizon. Previous research indicated that the signal can be used to detect normal and surface-based duct refractive conditions. Using reciprocity, we develop a model to predict ground-based GPS occultation signal propagation in the presence of marine tropospheric ducts. To involve the effects of refraction, sea surface impedance, roughness, and the Earth’s curvature, the parabolic equation (PE) method is adopted in the low atmosphere. In implementing the PE numerically using the split-step discrete mixed Fourier transform algorithm, we propose a new approach of utilizing the relationship between the impedance coefficient and the reflection coefficient to modeling the right-hand circularly polarized GPS signal. The refraction effects from the top of the PE region to the satellite are modeled by ray tracing technique....
- Published
- 2014
48. Visual acuity of young men in China
- Author
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Lei Shang, Yi Wan, Yong-Yong Xu, Yang Cao, Xun Jiang, Jie-Qiong Zhang, Ning-Xu Chen, and Jin-Peng Zhang
- Subjects
education.field_of_study ,medicine.medical_specialty ,Visual acuity ,genetic structures ,business.industry ,Cross-sectional study ,Public health ,Population ,General Medicine ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,medicine ,Cluster sampling ,Observational study ,030212 general & internal medicine ,medicine.symptom ,Young adult ,China ,education ,business ,Demography - Abstract
The prevalence of low vision has increased in China especially among youth population, which is an important public health issue. The trend on the prevalence of subnormal visual acuity and updated information is essential to quantify health effects and to prompt decision makers to prioritize action and assess the effectiveness of measures. Therefore, the study aimed to analyze the prevalence and geographical distribution of visual acuity level among young men in China based on 3 national cross-sectional surveys from 1974 to 2012.The data on visual acuity of young men were collected from 3 national surveys among military recruit youth conducted in 1974, 2001, and 2012 by using a stratified cluster sampling method in China. The prevalence of visual acuity among military recruit youth during this period was analyzed by region, year, age, and economic level.A total of 139,929, 72,894, and 58,106 young men were included, covering all 31 provinces of mainland of China, from the 3 national surveys respectively. The prevalence of subnormal visual acuity had geographic diversity and increased significantly from 1974 to 2012 (P < .05). The visual acuity level was negatively correlated with the age (17-23 years) in 2012 (P < .05). Furthermore, the prevalence of subnormal visual acuity was positively correlated with the gross domestic product in 31 provinces of China (P ≤ .001).The prevalence of subnormal visual acuity increased with economic development among young men from 1974 to 2012, with distinct variation among geographic areas in China. Furthermore, subnormal visual acuity was increasingly prevalent with age and warrant public health attention.
- Published
- 2019
49. Anti-androgen effects of the pyrethroid pesticide cypermethrin on interactions of androgen receptor with corepressors
- Author
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Ji-Long Zhou, Yan-fang Li, Qi Wang, Mei-Rong Zhang, Jin-Peng Zhang, Li-Fang Xu, Min Jiang, Hong-Min Yu, Chen Pan, Xiao-Long Zhou, Li-Chun Xu, Ya-Peng Liu, and Jin-xia Hu
- Subjects
Insecticides ,Thyroid hormone receptor ,Androgen Antagonists ,Haplorhini ,Biology ,Toxicology ,Molecular biology ,Cell Line ,Cypermethrin ,Androgen receptor ,N-terminus ,chemistry.chemical_compound ,Mediator ,Nuclear receptor ,chemistry ,Receptors, Androgen ,Protein Interaction Mapping ,Pyrethrins ,Animals ,Nuclear Receptor Co-Repressor 1 ,Gene silencing ,Nuclear Receptor Co-Repressor 2 ,Corepressor - Abstract
To clarify whether the mechanism of androgen receptor (AR) antagonism of the pyrethroid pesticide cypermethrin associates with the interactions between the AR and corepressors silencing mediator for thyroid hormone receptors (SMRT) and nuclear receptor corepressor (NCoR), we have developed the mammalian two-hybrid assays. The AR N-terminal domain 1-660 amino acid residues were subcloned into the plasmid pVP16 to construct VP16-ARNTD. The C-terminal receptor interaction domains (RIDs) of SMRT and NCoR were used to construct pM-SMRT and pM-NCoR. The constructed vectors pVP16-ARNTD, pM-SMRT or pM-NCoR, the reporter pG5CAT and the control pCMVβ were cotranfected into the CV-1 cells. The cells were treated with cypermethrin at the indicated concentrations. The AR N terminus interacted with RIDs of SMRT and NCoR. The interactions between the AR and corepressors SMRT and NCoR were enhanced by cypermethrin, and the significant enhancement was detected at the concentration of 10(-5)M. The mammalian two-hybrid assays demonstrate the utility to detect the interactions of the AR with SMRT and NCoR. Cypermethrin functions as an anti-androgen by enhancing the associations of the AR with SMRT and NCoR. We provide a novel mechanism in anti-androgen action of cypermethrin associated with the recruitment of SMRT and NCoR to AR.
- Published
- 2013
50. Research on functional and real-time fault-localization method for Flight Control software
- Author
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Ji-Bing Wang, Yang Yang, Ping Zhang, Jin-Peng Zhang, and Bei-Bei Yin
- Subjects
Engineering ,business.industry ,Reliability (computer networking) ,media_common.quotation_subject ,05 social sciences ,Real-time computing ,Process (computing) ,020207 software engineering ,02 engineering and technology ,Fault (power engineering) ,Automation ,Visualization ,Software ,Software fault tolerance ,0502 economics and business ,0202 electrical engineering, electronic engineering, information engineering ,business ,Function (engineering) ,050203 business & management ,media_common - Abstract
Flight control software has higher requirements on reliability, as well as real-time performance. What's more, the fault of flight control software not only contains traditional functional fault, but also contains real-time fault. Thus, fault localization and removing for Flight Control software is a huge challenge for relative engineers. To locate and remove those faults effectively, this paper adopts Sober algorithm as the functional fault-localization algorithm for flight control software. Meanwhile, this paper proposes the module-level and function- level real-time fault-localization algorithms inspired by Sober algorithm. The experimental results indicate that all algorithms have obtained acceptable localization results. For further goals on increasing the automation level and the efficiency of software fault-localization process, we develop a fault-localization and visualization (FL&V) tool for flight control software at last.
- Published
- 2016
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