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Mechanical weeding augmented with automation technology should result in highly effective weeding systems. However, the interaction between weeding mechanisms and soil is not well understood. Moreover, soil is highly variable, which makes studying this interaction challenging. The main objective of this research was to develop a method to investigate the effects of mechanical tool-soil interaction on weeding performance for different operating conditions in a controlled environment. Experiments were conducted in an indoor soil bin with loam soil, and the weeding performance was studied using small wooden cylinders as simulated weed plants. The investigations featured a single cylindrical tine and a rotating tine mechanism, vertically oriented and inserted into the soil. The total width of soil disturbance and potential weeding rate were evaluated for the single cylindrical tine at different levels of three operating parameters: tine diameter (6.35, 7.94, and 9.53 mm), working soil depth (25.4, 50.8, and 76.2 mm), and tine speed (0.23 and 0.45 m s-1). Potential weeding rate was examined for the rotating tine mechanism with two operating parameters: working soil depth (25.4 and 76.2 mm) and rotational speed (25, 50, and 100 rpm). Statistical analysis was performed using ANOVA at p < 0.05. A simulation of the rotating tine mechanism was developed that estimated the disturbed area. For the single tine, soil disturbance width was independent of tine speed; however, tine diameter and depth had significant effects, as the width increased with increased levels of these two parameters. All three parameters had significant effects on the potential weeding rate of the single tine, and the rates were observed to increase with higher levels of the parameters. For the rotating tine mechanism, both depth and rotational speed were significant. The potential weeding rate for the rotating tine mechanism was found to increase with higher levels of these parameters. The results showed that although the width of soil disturbance due to a cylindrical tine was affected by the tine diameter and working soil depth, operating parameters such as increased longitudinal and rotational speeds also affected plant disturbance. The percentage of disturbed soil area in the simulation followed similar patterns as the percentage of disturbed plants observed in the experiments. Keywords: Inter-row weeding, Intra-row weeding, Mechanical weeding, Rotating tine mechanism, Soil disturbance, Tine.