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The unique physical profile of each player's role in sub-elite women's cricket is vital for optimizing performance as these players progress to the elite levels. This quantitative, cross-sectional study investigates the physical profiles of sub-elite women's cricket players as a group and compares these profiles across different player roles. Sub-elite female cricket players in the South African domestic women's cricket league were included in this study. A battery of physical assessments were conducted at the start of the 2022/23 season. The physical assessments included body composition, individual muscle strength testing using dynamometry, 2-km time trial (TT), countermovement jump (CMJ), single-leg jump (SLJ), isometric mid-thigh pull, push-up, and hop test on force plates. A total of 44 female players (20.86 ± 1.6 years) were included in the study. Differences were found in muscle mass (p = 0.004) and peak power (p = 0.040) for all-rounders and bowlers. Player roles presented with different dominant (p = 0.006) and non-dominant (p = 0.066) knee flexion strength. The bowlers' body composition and physical strength profile are compromised compared with batters and all-rounders. There were several physical strength and power differences between pace and spin bowlers in CMJ and SLJ tests for jump height (p = 0.009) and peak power (p = 0.006). Batters performed the best in the 2-km TT. Body composition and musculoskeletal profiles for each player role can be baseline markers in sub-elite women's cricket. Stakeholders can use this information to guide physical preparation for players advancing to elite levels. [ABSTRACT FROM AUTHOR]