137 results on '"Jahani‐Sherafat, Somayeh"'
Search Results
2. Alterations in the gut microbiota and their metabolites in human intestinal epithelial cells of patients with colorectal cancer
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Jahani-Sherafat, Somayeh, Azimirad, Masoumeh, Raeisi, Hamideh, Azizmohammad looha, Mehdi, Tavakkoli, Sajjad, Ahmadi Amoli, Hamed, Moghim, Sharareh, Rostami-Nejad, Mohammad, Yadegar, Abbas, and Zali, Mohammad Reza
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- 2024
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3. Overview of the compromised mucosal integrity in celiac disease
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Taraz, Tannaz, Mahmoudi-Ghehsareh, Mohadeseh, Asri, Nastaran, Nazemalhosseini-Mojarad, Ehsan, Rezaei-Tavirani, Mostafa, Jahani-Sherafat, Somayeh, Naseh, Ali, and Rostami-Nejad, Mohammad
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- 2024
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4. The role of mir-197-3p in regulating the tight junction permeability of celiac disease patients under gluten free diet
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Asri, Nastaran, Fallah, Shayan, Rostami-Nejad, Mohammad, Fallah, Zahra, Khanlari-Kochaksaraei, Majid, Jafari-Marandi, Sayeh, Forouzesh, Flora, Shahrokh, Shabnam, Jahani-Sherafat, Somayeh, and Zali, Mohammad Reza
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- 2023
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5. Elevated interleukin-17A levels despite reduced microRNA-326 gene expression in celiac disease patients under gluten-free diet
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Nafari Maedeh, Asri Nastaran, Rostami-Nejad Mohammad, Forouzesh Flora, Ehsani-Ardakani Mohammad Javad, Jahani-Sherafat Somayeh, Rezaei-Tavirani Mostafa, and Aghdaei Hamid Asadzadeh
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autoimmune diseases ,celiac disease ,gene expression ,micrornas ,reverse transcriptase polymerase chain reaction ,Internal medicine ,RC31-1245 - Abstract
Introduction: The microRNA-326 (miR-326) gene, by targeting ETS Proto-Oncogene 1 (ETS1), regulates the differentiation and interleukin-17A production of T helper 17 (Th17) cells. Celiac disease (CD) is an intestinal autoimmune disorder, in which the cascade of Th17 cells plays an important role in its pathogenicity. The aim of this study was to evaluate the expression changes of miR-326 and its two target genes ETS1 and IL-17A in celiac disease patients under a gluten-free diet (GFD). We expected the expression of miR-326 and IL-17A gene to decrease, and the expression of the ETS1 gene to increase, following the adherence to GFD.
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- 2022
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6. The connection between fatty acids and inflammation in celiac disease; a deep exploring
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Bakhtiari, Sajjad, primary, Asri, Nastaran, additional, Jahdkaran, Mahtab, additional, Rezaei-Tavirani, Mostafa, additional, Jahani-Sherafat, Somayeh, additional, and Rostami-Nejad, Mohammad, additional
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- 2024
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7. The role of tryptophan metabolism and tolerogenic dendritic cells in maintaining immune tolerance: Insights into celiac disease pathogenesis.
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Asgari, Fatemeh, Khodadoust, Mahdi, Nikzamir, Abdolrahim, Jahani‐Sherafat, Somayeh, Rezaei Tavirani, Mostafa, and Rostami‐Nejad, Mohammad
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AMINO acid metabolism ,ESSENTIAL amino acids ,IMMUNOLOGICAL tolerance ,DENDRITIC cells ,PHENOTYPIC plasticity ,CELIAC disease - Abstract
Background: In mammals, amino acid metabolism has evolved to control immune responses. Tryptophan (Trp) is the rarest essential amino acid found in food and its metabolism has evolved to be a primary regulatory node in the control of immune responses. Celiac disease (CeD) is a developed immunological condition caused by gluten intolerance and is linked to chronic small intestine enteropathy in genetically predisposed individuals. Dendritic cells (DCs), serving as the bridge between innate and adaptive immunities, can influence immunological responses in CeD through phenotypic alterations. Objective: This review aims to highlight the connection between Trp metabolism and tolerogenic DCs, and the significance of this interaction in the pathogenesis of CeD. Results: It is been recognized that various DC subtypes contribute to the pathogenesis of CeD. Tolerogenic DCs, in particular, are instrumental in inducing immune tolerance, leading to T‐reg differentiation that helps maintain intestinal immune tolerance against inflammatory responses in CeD patients and those with other autoimmune disorders. T‐regs, a subset of T‐cells, play a crucial role in maintaining intestinal immunological homeostasis by regulating the activities of other immune cells. Notably, Trp metabolism, essential for T‐reg function, facilitates T‐reg differentiation through microbiota‐mediated degradation and the kynurenine pathway. Conclusion: Therefore, alterations in Trp metabolism could potentially influence the immune response in CeD, affecting both the development of the disease and the persistence of symptoms despite adherence to a gluten‐free diet. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Evaluating CD4 and Foxp3 mRNA Expression in Tissue Specimens of Celiac Disease and Colorectal Cancer Patients
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Asri, Nastaran, primary, Nazemalhosseini Mojarad, Ehsan, additional, Taleghani, Mohammad Yaghoob, additional, Houri, Hamidreza, additional, Saeedi Niasar, Mahsa, additional, Rezaei-Tavirani, Mostafa, additional, Jahani- Sherafat, Somayeh, additional, Shahbazkhani, Amirhossein, additional, Nikzamir, Abdolrahim, additional, Ehsani Ardakani, Mohammad Javad, additional, Khodadost, Mahmood, additional, and Rostami-Nejad, Mohammad, additional
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- 2024
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9. The link between gluten intake and the risk of cancers.
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Bakhtiari, Sajjad, Asri, Nastaran, Maleki, Sepehr, Rahimi, Saba, Jabbari, Amirreza, Ahmadzadeh, Alireza, Jahani-Sherafat, Somayeh, Farahani, Masoumeh, Mojarad, Ehsan Nazemalhosseini, and Rostami-Nejad, Mohammad
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TUMOR risk factors ,LYMPHOMA risk factors ,RISK assessment ,FOOD consumption ,COLORECTAL cancer ,AGE distribution ,CAUSES of death ,CHROMOSOME abnormalities ,GENE expression profiling ,GLUTEN ,CELIAC disease ,DISEASE risk factors - Abstract
Gluten is a complex mixture of hundreds of related proteins, with the two major groups being gliadin and glutenin. Gliadin primarily affects the viscosity of dough, while glutenin contributes to its strength. Nowadays, there is evidence suggesting an increase in gluten exposure due to advancements in cereal technology. Consumption of gluten can lead to development of gluten-related disorders (GRDs) in susceptible individuals. Some GRDs have been strongly associated with an increased risk of developing certain types of cancer. Colorectal cancer and lymphoma are among the most commonly reported malignancies associated with GRDs. Dietary factors, including gluten intake, have been recognized as significant modifiable risk factors for the development of digestive system cancers. The present study aimed to collect current information on the effect of gluten on the incidence of cancer in the general population and among GRDs patients. Protein-Protein Interaction (PPI) Network analysis of common genes between celiac disease (CD) and cancer was also conducted. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Evaluation of the Cellular Resistance Process in Treated Cells Via Extracorporeal Photopheresis
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Arjmand, Babak, primary, Rezaei Tavirani, Mostafa, additional, Jahani Sherafat, Somayeh, additional, Rezaei, Mitra, additional, Farahani, Masoumeh, additional, and Rezaei Tavirani, Majid, additional
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- 2023
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11. Application of Artificial Intelligence in Celiac Disease: from diagnosis to patient follow-up
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Mahmoudi Ghehsareh, Mohadeseh, additional, Asri, Nastaran, additional, Maleki, Sepehr, additional, Rezaei-Tavirani, Mostafa, additional, Jahani-Sherafat, Somayeh, additional, and Rostami-Nejad, Mohammad, additional
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- 2023
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12. The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses
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Ramedani, Najmeh, Seidita, Aurelio, Asri, Nastaran, Azimirad, Masoumeh, Yadegar, Abbas, Jahani-Sherafat, Somayeh, Sharifan, Anousheh, Mansueto, Pasquale, Carroccio, Antonio, Rostami-Nejad, Mohammad, Ramedani, Najmeh, Seidita, Aurelio, Asri, Nastaran, Azimirad, Masoumeh, Yadegar, Abba, Jahani-Sherafat, Somayeh, Sharifan, Anousheh, Mansueto, Pasquale, Carroccio, Antonio, and Rostami-Nejad, Mohammad
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Settore MED/09 - Medicina Interna ,non-celiac wheat sensitivity ,gliadin ,Caco-2 cells ,probiotic - Abstract
Background: Non-celiac wheat sensitivity (NCWS) is a poorly understood gluten-related disorder (GRD) and its prominent symptoms can be ameliorated by gluten avoidance. This study aimed to determine the effectiveness of a probiotic mixture in hydrolyzing gliadin peptides (toxic components of gluten) and suppressing gliadin-induced inflammatory responses in Caco-2 cells. Methods: Wheat dough was fermented with a probiotic mix for 0, 2, 4, and 6 h. The effect of the probiotic mix on gliadin degradation was monitored by SDS-PAGE. The expression levels of IL-6, IL-17A, INF-G, IL-10, and TGF-B were evaluated using ELISA and qRT-PCR methods. Results: According to our findings, fermenting wheat dough with a mix of B. longum, L. acidophilus, and L. plantarum for 6 h was effective in gliadin degradation. This process also reduced levels of IL-6 (p = 0.004), IL-17A (p = 0.004), and IFN-G (p = 0.01) mRNA, as well as decreased IL-6 (p = 0.006) and IFN-G (p = 0.0009) protein secretion. 4 h fermentation led to a significant decrease in IL-17A (p = 0.001)and IFN-G (p = 0.003) mRNA, as well as reduced levels of IL-6 (p = 0.002) and IFN-G (p < 0.0001) protein secretion. This process was also observed to increase the expression levels of IL-10 (p < 0.0001) and TGF-B (p < 0.0001) mRNA. Conclusions: 4 h fermentation of wheat flour with the proposed probiotic mix might be a good strategy to develop an affordable gluten-free wheat dough for NCWS and probably other GRD patients.
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- 2023
13. The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses
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Ramedani, Najmeh, primary, Seidita, Aurelio, additional, Asri, Nastaran, additional, Azimirad, Masoumeh, additional, Yadegar, Abbas, additional, Jahani-Sherafat, Somayeh, additional, Sharifan, Anousheh, additional, Mansueto, Pasquale, additional, Carroccio, Antonio, additional, and Rostami-Nejad, Mohammad, additional
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- 2023
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14. The effectiveness of photobiomodulation therapy in modulation the gut microbiome dysbiosis related diseases.
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Jahani-Sherafat, Somayeh, Taghavi, Hooman, Asri, Nastaran, Tavirani, Mostafa Rezaei, Razzaghi, Zahra, and Rostami-Nejad, Mohammad
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HOMEOSTASIS , *PHOTOBIOMODULATION therapy , *PATHOGENESIS , *GUT microbiome , *MICROBIOLOGY , *PHENOMENOLOGICAL biology , *INFLAMMATION , *CIRCADIAN rhythms , *TREATMENT effectiveness - Abstract
Maintaining a healthy balance between commensal, and pathogenic bacteria within the gut microbiota is crucial for ensuring the overall health, and well-being of the host. In fact, by affecting innate, and adaptive immune responses, the gut microbiome plays a key role in maintaining intestinal homeostasis and barrier integrity. Dysbiosis is the loss of beneficial microorganisms and the growth of potentially hazardous microorganisms in a microbial community, which has been linked to numerous diseases. As the primary inducer of circadian rhythm, light can influence the human intestinal microbiome. Photobiomodulation therapy (PBMT), which is the use of red (630-700 nm), and near-infrared light (700 and 1200 nm), can stimulate healing, relieve pain, and reduce inflammation, and affect the circadian rhythm and gut microbiome beneficially. Our focus in this paper is on the effects of PBMT on gut microbiota, to provide an overview of how it can help control gut microbiota dysbiosis-related disorders. [ABSTRACT FROM AUTHOR]
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- 2023
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15. The Clinical Effects of Laser Acupuncture on Hospitalized Patients With Severe COVID-19: A Randomized Clinical Trial
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Shojaeddin, Arista, primary, Fatemi, Alireza, additional, Razzaghi, Zahra, additional, Pishgahi, Mehdi, additional, Jahani Sherafat, Somayeh, additional, Razzaghi, Mohammadreza, additional, Shahrzad, Mohamad Karim, additional, Anaraki, Nafiseh, additional, Salehi, Chiman, additional, and Amiri, Aslan, additional
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- 2023
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16. Device-associated infection rates and bacterial resistance in six academic teaching hospitals of Iran: Findings from the International Nocosomial Infection Control Consortium (INICC)
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Jahani-Sherafat, Somayeh, Razaghi, Maryam, Rosenthal, Victor D., Tajeddin, Elahe, Seyedjavadi, Simasadat, Rashidan, Marjan, Alebouyeh, Masoud, Rostampour, Maryam, Haghi, Arezo, Sayarbayat, Masoumeh, Farazmandian, Somayeh, Yarmohammadi, Tahere, Arshadi, Fardokht K., Mansouri, Nahid, Sarbazi, Mohammad R., Vilar, Mariano, and Zali, Mohammad R.
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- 2015
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17. The role of mir-197-3p in regulating the tight junction permeability of celiac disease patients under gluten free diet
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Asri, Nastaran, primary, Fallah, Shayan, additional, Rostami-Nejad, Mohammad, additional, Fallah, Zahra, additional, Khanlari-Kochaksaraei, Majid, additional, Jafari-Marandi, Sayeh, additional, Forouzesh, Flora, additional, Shahrokh, Shabnam, additional, Jahani-Sherafat, Somayeh, additional, and Zali, Mohammad Reza, additional
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- 2022
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18. The Effect of 2.45 GHz Electromagnetic Fields on Fear Memory Extinction in Male Rats
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Dehghani, Zobeideh, primary, Mahdavi, Seyed Mohammad, additional, Modarresi Chahardehi, Amir, additional, Mansouri, Vahid, additional, and Jahani Sherafat, Somayeh, additional
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- 2022
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19. The modulation of macrophage subsets in celiac disease pathogenesis
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Molaaghaee‐Rouzbahani, Sara, primary, Asri, Nastaran, additional, Jahani‐Sherafat, Somayeh, additional, Amani, Davar, additional, Masotti, Andrea, additional, Baghaei, Kaveh, additional, Yadegar, Abbas, additional, Mirjalali, Hamed, additional, and Rostami‐Nejad, Mohammad, additional
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- 2022
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20. The Effect of Gluten-Free Diet Duration on Body Mass Index of Iranian Patients with Celiac Disease
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Asri, Nastaran, primary, Taraghikhah, Nazanin, additional, Baniasadi, Reihaneh, additional, Ishaq, Sauid, additional, Rezaei-Tavirani, Mostafa, additional, Sadeghi, Amir, additional, Ehsani-Ardakani, Mohammad-Javad, additional, Jahani-Sherafat, Somayeh, additional, Asadzadeh Aghdaei, Hamid, additional, and Rostami-Nejad, Mohammad, additional
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- 2022
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21. A trend of Medical Negligence in Laser Therapy in the Capital City: A Nine-Year Survey
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Jahani- Sherafat, Somayeh, primary, Razzaghi, Zahra, additional, Mahdavi, Seyed Amirhossein, additional, Forouzesh, Mehdi, additional, Emam Hadi, Mohammad Ali, additional, Rostami-Nejad, Mohammad, additional, Rezaei Tavirani, Mostafa, additional, and Razzaghi, Mohammadreza, additional
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- 2022
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22. Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
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Khalkhal, Ensieh, primary, Rezaei-Tavirani, Mostafa, additional, Asri, Nastaran, additional, Nobakht, Fatemeh, additional, Jahani-Sherafat, Somayeh, additional, Haidari, Mohammad Hosain, additional, and Rostami-Nejad, Mohammad, additional
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- 2022
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23. The Principal Role of Several Members of HLA and IRF Genes in Prevention of Oral Mucositis After Chemoradiotherapy
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Arjmand, Babak, primary, Khodadost, Mahmood, additional, Jahani Sherafat, Somayeh, additional, Rezaei Tavirani, Mostafa, additional, Ahmadi, Nayebali, additional, Hamzeloo Moghadam, Maryam, additional, Okhovatian, Farshad, additional, and Rezaei Tavirani, Sina, additional
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- 2021
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24. Low-Level Laser Therapy Effects on Rat Blood Hemostasis Via Significant Alteration in Fibrinogen and Plasminogen Expression Level
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Arjmand, Babak, primary, Khodadost, Mahmood, additional, Jahani Sherafat, Somayeh, additional, Rezaei Tavirani, Mostafa, additional, Ahmadi, Nayebali, additional, Okhovatian, Farshad, additional, and Rezaei Tavirani, Majid, additional
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- 2021
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25. Side effects of omeprazole: a system biology study
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Hamzeloo-Moghadam, Maryam, Rezaei Tavirani, Mostafa, Jahani-Sherafat, Somayeh, Rezaei Tavirani, Sina, Esmaeili, Somayeh, Ansari, Mojtaba, and Ahmadzadeh, Alireza
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System biology ,Original Article ,Network analysis ,Omeprazole - Abstract
Aim: To assess the effects of omeprazole on the human cardiovascular system is the main aim of this study. Background: Omeprazole as a proton pump inhibitor is widely consumed to inhibit gastric acid secretion. Methods: Gene expression profiles of “human coronary artery endothelial cells” in the absence and presence of omeprazole were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) interacted as an interactome, and the hub nodes are determined. The DEGs were enriched via gene ontology (GO) analysis. The critical hubs were identified based on the GO findings. Results: Among 103 queried DEGs, 61 individuals were included in the main connected component. CTNNB1, HNRNPA1, SRSF4, TRA2A, SFPQ, and RBM5 genes were identified as critical hub genes. Six clusters of biological terms were introduced as deregulated elements in the presence of omeprazole. Conclusion: In conclusion, long-term consumption of omeprazole may be accompanied with undesirable effects, however more evidence is required.
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- 2021
26. Low-Level Laser Therapy: Potential and Complications
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Arjmand, Babak, primary, Khodadost, Mahmood, additional, Jahani Sherafat, Somayeh, additional, Rezaei Tavirani, Mostafa, additional, Ahmadi, Nayebali, additional, Hamzeloo Moghadam, Maryam, additional, Okhovatian, Farshad, additional, Rezaei Tavirani, Sina, additional, and Rostami-Nejad, Mohammad, additional
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- 2021
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27. Gene expression profile analysis of colon cancer grade II into grade III transition by using system biology
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Rostami-Nejad, Mohammad, Rezaei Tavirani, Sina, Mansouri, Vahid, Jahani-Sherafat, Somayeh, and Moravvej Farshi, Hamideh
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biomarker ,Original Article ,gene ,Colon cancer - Abstract
Aim: Gene expression profile analysis of colon cancer grade II into grade III transition by using system biology. Background: Colon cancer is one of lethal cancer in men and women. Treatment in advanced colon cancer is difficult and survival rate is low. Methods: Gene expression profiles of children patients with non-preforated appendicitis in comparison with the samples with non- appendicitis abdominal pain are analysis via protein – protein interaction PPI and the critical compounds are introduced by STITCH. Results: Six critical genes including MAPK3, AKT1, SRC, TP53, GAPDH, and ALB were identified as a possible biomarker panel related to colon cancer grade II to III transition. Among these critical genes roles of MAPK3, AKT1, SRC, TP53 are highlighted. Conclusion: It was concluded that target therapy to regulate SRC and TP53 may be the effective therapeutic way to treatment of colon cancer and more researches in necessary to design drugs for these purposes.
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- 2019
28. The rate and importance of Clostridium difficile in colorectal cancer patients
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Jahani-Sherafat, Somayeh, Azimirad, Masoumeh, Alebouyeh, Masoud, Ahmadi Amoli, Hadi, Hosseini, Parnian, Ghasemian-Safaei, Hajieh, and Moghim, Sharareh
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Intestinal microbiota ,Original Article ,Clostridium difficile ,Toxin ,CRC - Abstract
Aim: The aim of this study was to analyze the Clostridium difficile and their toxins in cancerous tissues in comparison to their adjacent healthy tissues in patients with colorectal cancer (CRC) in Iran. Background: Intestinal infection or colonization by microbial pathogens and their released metabolites may have a role in the exacerbation of CRC. Methods: A total of 60 biopsy samples from 30 cancerous and 30 adjacent healthy tissues were collected from patients with CRC. Biopsies were homogenized and cultured in cycloserine cefoxitin fructose agar-agar medium to investigate the presence of C. difficile. DNA was extracted, PCR was performed on pure colonies for bacteria detection, and toxin genes were evaluated in each bacterium positive cases. Real-time PCR was performed on extracted DNA for quantitative comparison of Clostridium difficile in healthy and tumor tissues in CRC patients. Results: Clostridium difficile was isolated from 18 of the cancerous tissue (60%) and 6 of their healthy adjacent tissue (20%) in the culture medium, but toxin genes were positive just in one sample in both groups. Real-time PCR showed the colonization in all samples. Conclusion: This study showed a higher prevalence of Clostridium difficile in cancerous lesions in comparison to healthy tissues. We suggest that the investigation of the rate of CD of colorectal cancer patients before surgery is critical for patients. Further studies with more samples size to study the importance of this bacterium and its toxins in the investigation of colorectal cancer patients survey is recommended.
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- 2019
29. Introducing GATA3 as a prominent player in Crohn's disease
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Rezaei-Tavirani, Sina, Asri, Nastaran, Emamhadi, MohammadAli, Jahani-Sherafat, Somayeh, Seyed Salehi, Ali, Gholamrezaei, Zahra, and Lak, Elena
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Crohn’s disease ,Genes ,Original Article ,Gene ontology ,GATA3 transcription factor - Abstract
Aim: This study was aimed at gene assessment of Crohn's disease (CD) through protein-protein interaction (PPI) network analysis to find crucial genes. Background: CD is a major subtype of inflammatory bowel diseases (IBD), which affects gastrointestinal tract. PPI network analysis is a suitable tool to clarify a critical gene as a drug target or diagnostic biomarker for these types of diseases. Methods: Gene expression profile GSE126124 of 20 CD patients and 20 healthy controls was obtained from the Gene Expression Omnibus (GEO) database. RNA profile of peripheral blood mononuclear cells (PBMCs) and colon biopsy samples of the studied groups was investigated. Crucial genes were selected and analyzed via the PPI network by Cytoscape software. Gene ontology enrichment for the hubs, bottlenecks, and hub-bottlenecks was performed via CluGO plugin of Cytoscape software. Results: Eighty-one differentially expressed genes (DEGs) among 250 initial DEGs were highlighted as significant by FC>2 and p-value ≤ 0.05, and 69 significant DEGs were used for PPI network construction. The network was characterized by poor connections, so 20 top neighbors were added to form a scale-free network. The main connected component included 39 query DEGs and 20 added first neighbors. Three clusters of biological processes associated with crucial genes were identified and discussed. Conclusion: The results of this study indicated that GATA3 has a key role in CD pathogenesis and could be a possible drug target or diagnostic biomarker for Crohn’s disease.
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- 2021
30. Prevalence and outcome of COVID-19 among Iranian celiac patients.
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Gholam-Mostafaei, Fahimeh Sadat, Asri, Nastaran, Parvani, Naser, khamene, Elham Aghamohammadi, Barzegar, Farnoosh, Rostami-Nejad, Mohammad, Rezaei-Tavirani, Mostafa, Shahbazkhani, Bijan, Jahani-Sherafat, Somayeh, Rostami, Kamran, and Zali, Mohammad Reza
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COVID-19 ,MYALGIA ,FEVER ,CROSS-sectional method ,ANTIVIRAL agents ,CELIAC disease ,SEVERITY of illness index ,RISK assessment ,DYSPNEA ,HOSPITAL care ,DESCRIPTIVE statistics ,ANOREXIA nervosa ,HYDROXYCHLOROQUINE ,DATA analysis software - Abstract
Aim: This study aimed to evaluate the prevalence and outcome of COVID-19 among Iranian celiac disease patients. Background: Patients with celiac disease (CD) might be at greater risk for opportunistic viral infections. Coronavirus disease-2019 (COVID-19) is a new coronavirus (SARS-CoV-2) cause of respiratory disorder which spread around the world at the end of 2019. The question is does COVID-19 infection increase the risk of severe outcome and/or a higher mortality in treated celiac disease?. Methods: Data regarding demographic details, clinical history, and COVID-19 infection symptoms among treated celiac disease patients was collected from July 2020 to January 2021 and analyzed using SPSS version 25. Results A total of 455 celiac disease patients were included in this study. The prevalence of Covid-19 infection among celiac disease patients was 2.4%. Infection among women (72.7%) was higher than the men, and only one overweight man who smoked was hospitalized. Among COVID-19 infected celiac disease patients, the most common symptoms were myalgia 90.9% (10/11), fever, body trembling, headache, shortness of breath, loss of smell and taste, and anorexia (72.7%). Treatments for COVID-19, included antibiotics (90.9%), pain analgesics (54.5%), antihistamines (27.3%), antivirals (9.1%) and hydroxychloroquine (9.1%). Conclusion: This study shows that treated celiac disease is not a risk factor for severity or higher mortality in patients infected with COVID-19. Women, however, might need extra-protection to prevent COVID-19 infection. [ABSTRACT FROM AUTHOR]
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- 2022
31. The Effectiveness of Photobiomudulation therapy (PBMT) in COVID-19 Infection
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Jahani Sherafat, Somayeh, primary, Razaghi, Zahra, additional, Rostami-Nejad, Mohammad, additional, Rezaei Tavirani, Mostafa, additional, and Razzaghi, Mohammadreza, additional
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- 2020
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32. Seroprevalence of Toxoplasma gondii, HBV and HCV infections
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Rostami-Nejad, Mohammad, primary, Mohebbi, Seyed-Reza, additional, Jahani-Sherafat, Somayeh, additional, Barzegar, Farnoush, additional, Asadzade-Aghdaei, Hamid, additional, Sadeghi, Amir, additional, and Zali, Mohammad-Reza, additional
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- 2018
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33. The effect of intestinal microbiota metabolites on HT29 cell line using MTT method in patients with colorectal cancer.
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Jahani-Sherafat, Somayeh, Azimirad, Masoumeh, Ghasemian-Safaei, Hajieh, Amoli, Hadi Ahmadi, Moghim, Sharareh, Sherkat, Ghazal, and Zali, Mohammad Reza
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CELL proliferation , *BIOLOGICAL assay , *BIOPSY , *CELL culture , *CELL lines , *COLON tumors , *COLORIMETRY , *CULTURE media (Biology) , *SULFUR compounds , *TIME , *GUT microbiome , *CROSS-sectional method , *CELL survival ,RECTUM tumors - Abstract
Aim: The aim of this study was to evaluate the effect of intestinal microbiota metabolites in colorectal cancer patients on HT29 cell line using MTT assay. Background: Colorectal cancer is one of the most common malignant tumors. Human guts harbor abundant microbes that adjust many aspects of the host physiology. Increasing studies suggest that gut microbiota play a significant role in the incidence and expansion of CRC, as a result of virulence factors, bacterial metabolites, or inflammatory pathways. Methods: In this cross-sectional study, 60 biopsy samples including 30 cancerous and 30 adjacent healthy tissues were collected from patients with CRC during 2017. Biopsy samples were first cultured on Thioglycollate broth medium for 24hr after which the microbiota metabolites were filtered and stored at -20 C° for further evaluation. HT29 cells were treated by microbiota metabolites at different times (3, 6, 12, 18h) and its viability was assessed by MTT assay. Results: The cells treated with microbiota metabolites showed increased viability and proliferation in time-dependent analysis by MTT assay, but there was not significant differences between the two groups. Conclusion: It seems that microbial metabolites are able to induce proliferation and increase cell viability and thus induce colorectal cancer. [ABSTRACT FROM AUTHOR]
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- 2019
34. Comparison between medical therapy and surgical approach in treatment of patients with anal fissures; a randomized clinical trial study
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Shishegar, Azita, primary, Ghalkhani, Foorozan, additional, Vasei, Narges, additional, and Jahani-Sherafat, Somayeh, additional
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- 2016
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35. Role of gut microbiota in the pathogenesis of colorectal cancer; a review article.
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Jahani-Sherafat, Somayeh, Alebouyeh, Masoud, Moghim, Sharareh, Amoli, Hamed Ahmadi, and Ghasemian-Safaei, Hajieh
- Subjects
- *
ANAEROBIC bacteria , *CANCER patients , *COLON tumors , *GUT microbiome ,RECTUM tumors - Abstract
Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide. Lifestyle is identified as one of the most important risk factors for CRC, especially in sporadic colorectal cancer. The natural composition of the gut microbiota changes rapidly during the first decade of life. Maintaining homeostasis in the gut is essential as structural and metabolic functions of the commensal microbiota inhibit gut colonization of pathogens. Dysbiosis, imbalance in function or structure of gut microbiota, has been associated with a variety of diseases, such as colorectal cancer. The aim of this review was to investigate the possible links between the dysbiosis in gut microbiota and colorectal cancer, and the potential role of anaerobic gut microbiota in the pathogenesis of colorectal cancer. Based on this review, various studies have shown that some of the gut microbiota such as anaerobic bacteria significantly increased in CRC patients, but we suggest more investigations are required to assess the importance of these bacteria and their metabolites in the pathogenesis of CRC are required. [ABSTRACT FROM AUTHOR]
- Published
- 2018
36. Common and differential genetically pathways between ulcerative colitis and colon adenocarcinoma.
- Author
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Akbari, Somayeh, Hosseini, Mostafa, Rezaei-Tavirani, Majid, Rezaei-Tavirani, Mostafa, Salehi, Seyed Hamid, Alemrajabi, Mahdi, Vaseghi-Maghvan, Padina, and Jahani-Sherafat, Somayeh
- Subjects
ADENOCARCINOMA ,ULCERATIVE colitis ,COLON tumors ,GENES ,ONTOLOGIES (Information retrieval) ,DISEASE prevalence ,GENETICS - Abstract
Aim: In the present study, genes of Ulcerative Colitis and Colon Adenocarcinoma (COAC) were extracted by string App in Cytoscape software version 3.5.1. Then protein- protein interaction (PPI) networks analyzed. Background: One of the most common chronic digestive problems is ulcerative colitis (UC) especially in developing countries. Prevalence of the disease is reported about 7.6 to 245 cases per 100,000 per year. UC can lead to colon cancer that is the third malignancy related death in the world. So awareness of the future of the patient with UC and the possibility of colon cancer is a very helpful approach. Methods: The analysis was based on centralities values. The goal is determining common gene pathways and differential gene pathways of the two diseases. Results: Results showed there are 11 and 29 central genes related to COAC and UC respectively. At least five common key genes between the two diseases were introduced. The number of 26 terms related to the common key genes were determined and clustered in seven clusters. Conclusion: ALB, AKT1, TP53, SRC and MYC are the common genes that play crucial roles in the related biological processes of UC and COAC. Besides introducing the common genes the differentiate genes related to the two diseases were proposed. [ABSTRACT FROM AUTHOR]
- Published
- 2017
37. The evaluation of functional results before and after laparoscopic Heller myotomy for achalasia: a single center experience.
- Author
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Pooshani, Abdollah, Frootan, Mojgan, Abdi, Saeed, Jahani-Sherafat, Somayeh, and Kamani, Freshteh
- Subjects
APHASIA ,ESOPHAGEAL achalasia ,GASTROINTESTINAL motility ,LAPAROSCOPIC surgery ,CROSS-sectional method - Abstract
Aim: The aim of this study was to evaluate and compare the functional results before and after laparoscopic Heller myotomy for Iranian patients with achalasia. Background: Achalasia is a severe neuromuscular disorder of the esophagus, characterized by the loss of peristalsis and an inability of the lower esophageal sphincter (LES) to reach optimal relaxation. Methods: In this cross sectional study, patients who underwent Heller myotomy for achalasia via laparoscopy in Taleghani Hospital Tehran, Iran were evaluated. Symptoms including pressure of residual, integrated relaxation sphincter (IRP), pressure of free drinking, pressure of LES, dysphasia score and peristalsis movement was measured and recorded by manometry before and after (2 months) treating with Heller myotomy. Results: In this study, 23 patients with achalasia (12 females and 11 males) with a mean age of 30±3.5 years (minimum 20, maximum 44 years) who met the inclusion criteria of the study were examined. Results of this study showed that, all the diagnostic criteria that were measured before the treatment was significantly different from after the treatment (P<0.05). The average decline in LES, IRP, Residual Pressure, Free drinking esophagus, and dysphasia score were 23.1 mmHg, 16.24 mmHg, 18,7 mmHg, 18.9 mmHg, and 5.0 unit, respectively. Also the average increase of the peristalsis movement was 8.26±13.7 in 8 patients. Conclusion: Considering the results of Heller myotomy surgery can be as a treatment of choice for achalasia. Free Drinking pressure can be a suitable criteria after treatment for evaluation and prediction of the reducing the dysphasia score after the surgery. [ABSTRACT FROM AUTHOR]
- Published
- 2017
38. The early efficacy of Heller myotomy in the treatment of Iranian patients with achalasia.
- Author
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Abdi, Saeed, Forotan, Mojgan, Nikzamir, Abdolrahim, Zomorody, Saeedeh, and Jahani-Sherafat, Somayeh
- Subjects
DEGLUTITION disorders ,ESOPHAGUS ,ESOPHAGEAL achalasia ,DEMOGRAPHY ,GASTROENTEROLOGY ,MANOMETERS ,T-test (Statistics) ,TREATMENT effectiveness ,DATA analysis software ,DESCRIPTIVE statistics ,SYMPTOMS ,DIAGNOSIS ,ANATOMY - Abstract
Aim: The purpose of this study was to determine the efficacy of Heller myotomy for the treatment of achalasia in a referral center in Tehran, and investigate the clinical characteristics, manometric results and treatment responses among three achalasia subtypes in Iranian patients. Background: Esophageal achalasia is an unusual swallowing disorder, characterized by high pressure in the lower esophageal sphincter (LES) on swallowing, failure relaxation of the LES and the absence of peristalsis in esophageal. Patients and methods: In this cross sectional study, clinical symptom and esophageal manometry before and 2 months after treating with Heller myotomy in 20 patients with achalasia who were referred to Taleghani Hospital, Tehran, in 2013 were evaluated. Patients' demographic, clinical features and response to treatment were analyzed using SPSS software (version 20, Chicago, IL, USA). Results: All the diagnostic criteria measured after the treatment were significantly different (P<0.05) before and after the therapy. The average decline in the length of the esophagus was 1.8 cm and dysphasia score was 7.25 units. Also an average decline in LES Resting Pressure, LES Residual Pressure, PIP, and IRP were 23.2 mmHg, 14.3 mmHg, 3.4 mmHg and 17.8 mmHg, respectively. Conclusion: Results of this study showed that the Heller myotomy is highly effective in relieving dysphasia in patients with achalasia. Also, type II achalasia is the most common subtype of achalasia with a better response to Heller myotomy compared to the other types. [ABSTRACT FROM AUTHOR]
- Published
- 2016
39. Lack of Association between Helicobacter pylori Infection and Biliary Tract Diseases
- Author
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JAHANI SHERAFAT, SOMAYEH, primary, TAJEDDIN, ELAHE, additional, REZA SEYYED MAJIDI, MOHAMMAD, additional, VAZIRI, FARZAM, additional, ALEBOUYEH, MASOUD, additional, MOHAMMAD ALIZADEH, AMIR HOOSHANG, additional, NAZEMALHOSSEINI MOJARAD, EHSAN, additional, and REZA ZALI, MOHAMMAD, additional
- Published
- 2012
- Full Text
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40. Association of Helicobacter pylori with central serous chorioretinopathy in Iranian patients.
- Author
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Roshani, Mohammad, Ahangar Davoodi, Nasrin, SeyyedMajidi, Mohammad Reza, Zojaji, Homayoun, Jahani Sherafat, Somayeh, Hashemi, Massih, and Zali, Mohammad Reza
- Subjects
DIAGNOSIS of eye diseases ,HELICOBACTER disease diagnosis ,RETINAL disease diagnosis ,RETINAL anatomy ,ACADEMIC medical centers ,GASTROENTEROLOGY ,HELICOBACTER pylori ,LONGITUDINAL method ,U-statistics ,DATA analysis ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Aim: This study was conducted to investigate a possible association between H. pylori infection and CSR. Background: Helicobacter pylori infection is one of the most common infections worldwide. Central Serous Chorioretinopathy (CSR) is a serious macular detachment that usually affects young people. The etiopathogenesis of the disease is still not completely understood. Patients and methods: A prospective study was performed and we evaluated a total of 35 CSR patients and control group of 138 patients who referred to gastroenterology research center of Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Central serous chorioretinopathy was diagnosed on the basis of findings in ophthalmic examinations and confirmed by fluoresce in angiogram. All patients underwent a 13C-urea breath test (UBT) to detect H. pylori infection. Patients were defined as H. pylori infected, if breath test was positive. Results: The mean duration of symptoms before diagnosis was 8.3± 2.5 days. Overall, no statistically significant difference was found between left and right eyes, bilateral CSR was observed in 5 patients (14.2%).The incidence of H. pylori infection was 85.7% in CSR patients and 55.1% in control subjects (p=0.001). Odd's ratio was 4.895. Conclusion: These results indicate that the prevalence of H. pylori infection is significantly higher in patients with CSR than in controls. No effect of age or sex was seen on H. pylori test results. Further multiple centers, randomized, case control trials are necessary to confirm the potential contributory role of the H. pylori infection in the pathogenesis of CSR as a possible association between infectious agents and endothelial dysfunction. [ABSTRACT FROM AUTHOR]
- Published
- 2014
41. The efficacy and safety of adding the probiotic Saccharomyces boulardii to standard triple therapy for eradication of H.pylori: a randomized controlled trial.
- Author
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Zojaji, Homayoun, Ghobakhlou, Mehdi, Rajabalinia, Hassan, Ataei, Elnaz, Jahani Sherafat, Somayeh, Moghimi-Dehkordi, Bijan, and Bahreiny, Rasoul
- Subjects
HELICOBACTER disease treatment ,THERAPEUTIC use of probiotics ,AMOXICILLIN ,CARCINOGENS ,COMBINATION drug therapy ,CHI-squared test ,CLINICAL trials ,ENDOSCOPY ,GASTROENTEROLOGY ,HELICOBACTER diseases ,T-test (Statistics) ,UREA ,PROTON pump inhibitors ,DATA analysis ,PROBIOTICS ,TREATMENT effectiveness ,TREATMENT duration ,DATA analysis software ,DESCRIPTIVE statistics ,CLARITHROMYCIN ,DISEASE complications ,EVALUATION - Abstract
Aim: Evaluating the efficacy and safety of adding the probiotic Saccharomyces boulardii to standard triple therapy for eradication of Helicobacter pylori. Background: Several probiotics such as Saccharomyces boulardii have been investigated for their clinical efficacy. This probiotic inhibit H. pylori urease by lowering the gastric pH, adhesion of H. pylori to gastric epithelial cells, stabilize the gastric barrier function and reduce the side effects of antibiotics. Patients and methods: In this randomized controlled trial we evaluated 160 adult patients with biopsy confirmed H. Pylori infection referred to gastroenterology ward of Taleghani hospital. The patients were randomized into two treatment regimens: patients in group A (n=80) were given amoxicillin (1000 mg, b.i.d), clarithromycin (500 mg, b.i.d), omeprazole (20 mg, b.i.d) and probiotic of saccaromyces boularidii (Yomogi) (250 mg, b.i.d) for 14 days. Moreover, patients in group B (n=80) were given amoxicillin (1000 mg, b.i.d), clarithromycin (500 mg b.i.d) and omeprazole (20 mg,b.i.d) for 14 days. Results: 160 patients (66 male 41.25%, 94 female 58.75%) with the mean age of 47.1±11.4 years were evaluated. The success rate for H. pylori eradication in group A was higher 75(87.5%) than group B 65 (81.2%), but the difference between two groups was not significant (p=0.350). Moreover, in case group, side effects such as nausea, diarrhea, abdominal discomfort and bloating were significantly lower than control group in first and second weeks. Conclusion: This study showed that saccaromyces boularidii decreased the adverse effects associated with H.pylori therapy but did not significantly increase the eradication rate of H.pylori. [ABSTRACT FROM AUTHOR]
- Published
- 2013
42. The effect of the treatment of Helicobacter pylori infection on the glycemic control in type 2 diabetes mellitus.
- Author
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Zojaji, Homayon, Ataei, Elnaz, Jahani Sherafat, Somayeh, Ghobakhlou, Mehdi, and Fatemi, Seyed Reza
- Subjects
HELICOBACTER disease diagnosis ,TYPE 2 diabetes diagnosis ,STOMACH ,ACADEMIC medical centers ,ANALYSIS of variance ,BIOMARKERS ,CLINICAL trials ,DEMOGRAPHY ,DIABETES ,ENDOCRINOLOGY ,GLYCOSYLATED hemoglobin ,HELICOBACTER diseases ,IMMUNOGLOBULINS ,EVALUATION of medical care ,METABOLIC regulation ,T-test (Statistics) ,DATA analysis ,DISEASE duration ,DESCRIPTIVE statistics ,DISEASE complications ,ANATOMY - Abstract
Aim: To evaluate the possible long-term effects of Helicobacter pylori infection on Hemoglobin A1c and fasting blood sugar levels in patients with type 2 diabetes. Background: Helicobacter pylori causes the gastrointestinal tract inflammation, which it plays an important role in distortion of glucose and lipids absorption that altered lipid metabolism and energy harvesting and develops type 2 diabetes, insulin resistance and has been linked to impaired blood glucose. Patients and methods: In this clinical trial, patients with type 2 diabetes and confirmed Helicobacter pylori infection were recruited from the endocrinology clinic of the Shahid Beheshti University Tehran, Iran. Before and after 3 months of eradication therapy fasting blood samples were taken and glycalated hemoglobin levels and fasting blood sugar levels were measured. Results: 85 (27 male 31.8%, 58 female 68.2%) patients with the mean age of 52.±4.7 years were recruited. 52 (62%) had successful Helicobacter pylori eradication (16 male, 30.8% and 36 female, 69.2%). The mean glycalated haemoglobin levels before successful treatment was 8.7±1.1 and after treatment was 8.3±0.9 and difference was significant (p<0.001). Mean IgG level of serology was 3.3±1.1 and the correlation with glycalated haemoglobin was significant (p=0.02) (r=0.4). Conclusion: Our results indicate that the Helicobacter pylori treatment can improve the mean glycalated haemoglobin in patients with type 2 diabetes. More investigations will be required to evaluate the effects of Helicobacter pylori eradication among different age groups and in relation to obesity status, diabetes and other disease, and it may be beneficial for patients at risk of diabetes to be checked for the presence of Helicobacter pylori infection. [ABSTRACT FROM AUTHOR]
- Published
- 2013
43. Non-Helicobacter pylori, non-NSAIDs peptic ulcers: a descriptive study on patients referred to Taleghani hospital with upper gastrointestinal bleeding.
- Author
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Rajabalinia, Hasan, Ghobakhlou, Mehdi, Nikpour, Shahriar, Dabiri, Reza, Bahriny, Rasoul, Jahani Sherafat, Somayeh, Moghaddam, Pardis Ketabi, and Alizadeh, Amirhoushang Mohammad
- Subjects
GASTROINTESTINAL hemorrhage diagnosis ,GASTROINTESTINAL disease diagnosis ,HELICOBACTER disease diagnosis ,KIDNEY stones diagnosis ,MEDICAL needs assessment ,ENDOSCOPY ,GASTROENTEROLOGY ,GASTROINTESTINAL hemorrhage ,PATIENT aftercare ,CIRRHOSIS of the liver ,RESEARCH methodology ,MEDICAL referrals ,NONSTEROIDAL anti-inflammatory agents ,PEPTIC ulcer ,COMORBIDITY ,DATA analysis ,DESCRIPTIVE statistics ,DISEASE complications ,SYMPTOMS - Abstract
Aim: The purpose of the present study was to evaluate the number and proportion of various causes of upper gastrointestinal bleeding and actual numbers of non-NSAID, non-Helicobacter pylori (H.pylori) peptic ulcers seen in endoscopy of these patients. Background: The number and the proportion of patients with non- H.pylori, non-NSAIDs peptic ulcer disease leading to upper gastrointestinal bleeding is believed to be increasing after eradication therapy for H.pylori. Patients and methods: Medical records of patients referred to the emergency room of Taleghani hospital from 2010 with a clinical diagnosis of upper gastrointestinal bleeding (hematemesis, coffee ground vomiting and melena) were included in this study. Patients with hematochezia with evidence of a source of bleeding from upper gastrointestinal tract in endoscopy were also included in this study. Results: In this study, peptic ulcer disease (all kinds of ulcers) was seen in 61 patients which were about 44.85% of abnormalities seen on endoscopy of patients. Among these 61 ulcers, 44 were duodenal ulcer, 22 gastric ulcer (5 patients had the both duodenal and gastric ulcers). Multiple biopsies were taken and be sent to laboratory for Rapid Urease Test and pathological examination. About 65.53% of patients had ulcers associated with H.pylori, 9.83% had peptic ulcer disease associated with NSAIDs and 11.47% of patients had ulcers associated with both H.pylori and consumption of NSAIDs. 13.11% of patients had non-NSAIDs non- H.pylori peptic ulcer disease. Conclusion: The results of this study supports the results of other studies that suggest the incidence of H.pylori infection related with duodenal ulcer is common, and that non-H pylori and non-NSAIDs duodenal ulcer is also common. [ABSTRACT FROM AUTHOR]
- Published
- 2012
44. The correlation between fecal microbiota profiles and intracellular junction genes expression in young Iranian patients with celiac disease.
- Author
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Mahmoudi Ghehsareh, Mohadeseh, Asri, Nastaran, Gholam-Mostafaei, Fahimeh Sadat, Houri, Hamidreza, Forouzesh, Flora, Ahmadipour, Shokoufeh, Jahani- Sherafat, Somayeh, Rostami-Nejad, Mohammad, Mansueto, Pasquale, and Seidita, Aurelio
- Abstract
Celiac disease (CD) is characterized by the disruption of the intestinal barrier integrity and alterations in the microbiota composition. This study aimed to evaluate the changes in the fecal microbiota profile and mRNA expressions of intracellular junction-related genes in pediatric patients with CD compared to healthy controls (HCs). Thirty treated CD patients, 10 active CD, and 40 HCs were recruited. Peripheral blood (PB) and fecal samples were collected. Microbiota analysis was performed using quantitative real-time PCR (qPCR) test. The mRNA expressions of ZO-1, occludin, β-catenin, E-cadherin, and COX-2 were also evaluated. In active and treated CD patients, the PB expression levels of ZO-1 (
p = 0.04 and 0.002, respectively) and β-catenin (p = 0.006 and 0.02, respectively) were lower than in HCs. PB Occludin’s level was upregulated in both active and treated CD patients compared to HCs (p = 0.04 and 0.02, respectively). However, PB E-cadherin and COX-2 expression levels and fecal mRNA expressions of ZO-1, occludin, and COX-2 did not differ significantly between cases and HCs (P˃0.05). Active CD patients had a higher relative abundance of theFirmicutes (p = 0.04) andActinobacteria (p = 0.03) phyla compared to treated subjects. The relative abundance ofVeillonella (p = 0.04) andStaphylococcus (p = 0.01) genera was lower in active patients in comparison to HCs. Researchers should explore the precise impact of the gut microbiome on the molecules and mechanisms involved in intestinal damage of CD. Special attention should be given toBifidobacteria and Enterobacteriaceae, as they have shown a significant correlation with the expression of tight junction-related genes. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
45. The Effect of Photobiomodulation and Akkermansia muciniphila on THP-1 Derived Macrophage Polarization Treated with Gliadin Peptide.
- Author
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Jahani-Sherafat S, Mollaghaei S, Asri N, Rezaei Tavirani M, Baghaei K, and Rostami-Nejad M
- Abstract
Introduction: Photobiomodulation (PBM) and Akkermansia muciniphila have been shown to be effective in improving inflammatory conditions with positive effects on increasing the population of anti-inflammatory M2 macrophages (MQs). In this study, gliadin-stimulated THP-1 derived MQs were treated with A. muciniphila and PBM to evaluate their effects on promoting the polarization of M2 MQs. Methods: The human monocyte cell line (THP-1) was differentiated to MQs. MQs were stimulated with 200 μg/mL gliadin for 24 hours and then treated with PBM 810 nm alone and in combination with A. muciniphila for the following 24 hours to evaluate their effects on MQs polarization. THP-1 derived MQs were also treated with PBM and A. muciniphila to evaluate their effects on non-stimulated MQs. CD11b, CD80, and CD206 levels were evaluated by using the flow cytometry technique. Moreover, the expression of some M1 and M2-related cytokines was determined. Results: PBM therapy of gliadin-stimulated MQs decreased IL-6 and increased TGF-β, IL-10 and TNF-α expression compared with gliadin exposed MQs. PBM along with A. muciniphila treatment induced IL-6, TNF-α, and IL-10 expression in MQs in comparison to the untreated group. It also elevated TGF-β, IL-10 and TNF-α levels in gliadin-triggered MQs in comparison to gliadin-stimulated MQ cells. Conclusion: The result of this study showed the potential of PBMT and A. muciniphila for modulating inflammatory responses and MQs polarization. This may open new perspectives to find possible therapeutic targets for celiac diseases., Competing Interests: No potential conflict of interest was reported by the authors., (Copyright © 2024 J Lasers Med Sci.)
- Published
- 2024
- Full Text
- View/download PDF
46. Assessment of Recovery Time Effects on Human Primary Neonatal Dermal Fibroblasts After Exposure to Solar-Simulated Ultraviolet Radiation.
- Author
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Bandarian F, Razi F, Rezaei-Tavirani M, Arjmand B, Jahani Sherafat S, and Rostami Nejad M
- Abstract
Introduction: Photoaging that is accompanied by gene expression alteration is known as early aging of the skin due to overexposure to natural and/or artificial ultraviolet radiation (UVR). The assessment of gene expression alteration in human primary neonatal dermal fibroblasts depending on recovery time after exposure to solar simulated ultraviolet radiation (ssUVR) is the main aim of this bioinformatic study. Methods: Data are extracted from Gene Expression Omnibus (GEO). The pre-evaluation is done via the GEO2R program. The Significant differentially expressed genes (DEGs) were assessed via protein-protein interaction (PPI) network analysis, and the central genes were identified. The central genes were enriched via gene ontology assessment. Results: Among 224 significant DEGs, 20 central genes including TOP2A, MKI67, BRCA1, HELLS, MAD2L1, ANLN, KIF11, MSH2, KRAS, NCAPG, RFC3, PLK4, WDHD1, BLM, CDKN3, KIF15, SMARCA5, and ATAD2 as hub genes and TOP2A, MKI67, BRCA1, ANLN, KRAS, PLK4, SMARCA5, MMP2, and TLR4 as bottleneck genes were determined. Eight central genes were associated with 16 biological terms. Conclusion: In conclusion, significant differences appeared between gene expression conditions of the cells after 1-day and 5-day recovery. Molecular events include the repair and continuation of photodamages. It is possible to introduce drug targets to prevent the progress of induced damages., Competing Interests: The authors declare they have no conflicts of interest, (Copyright © 2024 J Lasers Med Sci.)
- Published
- 2024
- Full Text
- View/download PDF
47. Introducing critical proteins related to liver ischemia/reperfusion injury.
- Author
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Arjmand B, Khodadoost M, Jahani Sherafat S, Rezaei Tavirani M, Ahmadi N, and Rezaei Tavirani S
- Abstract
Aim: The current study aimed to introduce the key proteins involved in liver ischemia/reperfusion (I/R) injury through protein-protein interaction (PPI) analysis., Background: Liver transplantation (LT) is a well-known treatment for liver diseases that threaten patients with mortality. LT is a complex operation, and several risks, including liver I/R injury, affect its success. Improving LT requires detection of its molecular mechanism. Experiments have revealed that high throughput methods such as proteomics in combination with bioinformatics are useful tools for analyzing the molecular mechanism of disease., Methods: The differentially expressed proteins (DEPs) involved in liver I/R injury were extracted from the literature. The queried DEPs plus the first 100 neighbors were included in a network through STRING database using Cytoscape software. Degree, betweenness centrality, closeness centrality, and stress were considered to determine the central nodes. The queried DEPs were assessed by action map analysis using the CluePedia application of Cytoscape software. The key proteins were identified by comparing network analysis and action map evaluation results., Results: Six proteins, namely ALB, INS, GAPDH, CAT, IL6, and TNF, among the added first neighbors were determined as the central first neighbors. MPO, CRP, MMP9, and HMOX1 were selected as central DEPs among the queried proteins. Action map analysis confirmed the PPI findings. The final evaluation revealed that MMP9 in combination with CRP and HMOX1 plays a critical role in liver I/R injury., Conclusion: The significant role of MMP9 in liver I/R injury was detected in this study. Two central proteins (CRP and HMOX1) were shown to have a regulatory effect on MMP9; CRP activated MMP9, while HMXO1 downregulated it., Competing Interests: The authors declare no conflict of interest., (© 2024, Gastroenterology and Hepatology From Bed to Bench (GHFBB).)
- Published
- 2024
- Full Text
- View/download PDF
48. Targeting colon cancer via antimicrobial RT2 peptide: a system biology study.
- Author
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Hosseinpour Z, Zamanian Azodi M, Jahani Sherafat S, and Rezaei Tavirani M
- Abstract
Aim: This study aims to investigate the anticancer molecular mechanism of RT2 through protein-protein interaction (PPI) network analysis. For this aim, a bioinformatics evaluation of the proteome profile of colon cancer is carried out., Background: Antimicrobial peptides such as RT2 showed anticancer properties against various tumors. The molecular mechanism of the anticancer effect of RT2 is a challenging subject., Methods: By applying Cytoscape V.3.9.1 and integrated apps, the profile of the interaction network and related centrality is analyzed. An enrichment analysis of hub bottlenecks was also performed, and highlighted biological processes were visualized and determined., Results: Several 207 differentially expressed proteins were retrieved by PPI network analysis, and 10 hub bottlenecks were introduced. Among these differentially expressed proteins (DEPs), only AKT1 is from the queried DEPs. Key biological processes contributing to RT2 targeting mechanism include "Regulation of fibroblast proliferation", "Positive regulation of cyclin-dependent protein serine/threonine kinase activity", "positive regulation of miRNA transcription", and "fungiform papilla formation"., Conclusion: In conclusion, central proteins Tp53, MYC, EGFR, AKT1, HDAC1, and SRC can be introduced as a targeted biomarker panel of bioactive peptide treatments. However, extensive research is required to establish this claim before clinical application., Competing Interests: The authors declare no conflict of interest., (© 2023, Gastroenterology and Hepatology From Bed to Bench (GHFBB).)
- Published
- 2023
- Full Text
- View/download PDF
49. Network analysis of liver cancer: a system biology approach.
- Author
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Arjmand B, Jahani Sherafat S, Rezaei Tavirani M, Hamzeloo Moghadam M, and Abbasi MA
- Abstract
Aim: Determining critical dysregulated proteins in liver cancer was the main aim of this study., Background: Liver cancer is a common health problem characterized by difficulties in early diagnosis and rapid progression. Due to the lack of targeted drugs and the other features of the disease, the survival rate for patients is extremely low., Methods: The related dysregulated proteins for liver cancer were retrieved from the STRING database. The queried proteins were included in a network by Cytoscape software, and the central nodes of the network were enriched via gene ontology., Results: Among 11 introduced central nodes (GAPDH, TP53, EGFR, MYC, INS, ALB, IL6, AKT1, VEGFA, CDH1, and HRAS), HRAS and AKT1 were highlighted as critical dysregulated proteins which can be considered as possible biomarkers., Conclusion: Analysis revealed that AKT1, HRAS and the related biochemical pathways (especially "HIF-1 signaling pathway") are the possible diagnostic and therapeutic agents of liver cancer., Competing Interests: There is no conflict of interest., (© 2023, Gastroenterology and Hepatology From Bed to Bench (GHFBB).)
- Published
- 2023
- Full Text
- View/download PDF
50. Down-regulation of TP53 is a highlighted molecular event in gastric ulcer.
- Author
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Vafaee R, Jahani Sherafat S, Rezaei Tavirani M, and Ahmadi N
- Abstract
Aim: The current study explored the crucial dysregulate proteins and biochemical pathways in gastric ulcer as its main aim., Background: Gastric ulcer as an acid-related gastrointestinal disease is known as one of the most public gastrointestinal disorders., Methods: A total of 100 proteins from STRING database were analyzed by Cytoscape and its applications to find the central proteins and the related biochemical pathways. Action map analysis was applied to explore regulatory relationships between the critical proteins., Results: Network analysis and gene ontology revealed that IL6, ALB, TNF, INS, IL1B, IL10, TP53, CXCL8, and PTGS2 are the highlighted proteins related to gastric ulcer. Six clusters of biochemical pathways, namely "response to external stimulus," "multicellular organismal process," "regulation of biological quality," "cellular response to stimulus," "cellular response to chemical stimulus," and "transport" were identified as the dysregulated pathway in patients., Conclusion: Down-regulation of TP53 by IL2, PTGS2, and TNF seems to be a main process occurring in gastric ulcer patients., Competing Interests: The authors declare that they have no conflict of interest., (©2022 RIGLD, Research Institute for Gastroenterology and Liver Diseases.)
- Published
- 2022
- Full Text
- View/download PDF
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