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Human-caused mortality is a major cause of decline for large carnivore populations worldwide. Here we use a long-term data compiled from 1980 to 2018 to highlight the possible linkages between conflict-related mortality of lions (Panthera leo) and diverse socioecological factors in the Greater Etosha Landscape (GEL) surrounding Etosha National Park (ENP) in northwestern Namibia. We also use conflict-related mortality records (2001–2018) to model the spatial risk of fatality for lions from anthropogenic mortality, identify the major predictors of mortality, and map potential hotspots of anthropogenic mortality across the GEL. There were 698 conflict related lion mortalities reported between 1980 and 2018 with an average annual mortality of 22 lions (SE 16), although these are likely underestimates as not all anthropogenic mortality may have been reported. Conflict-related anthropogenic mortality of lions peaked during the cold dry season when availability of water was limited and game movements were concentrated around perennial waterpoints, and was greater in areas with localized livestock populations and low woody cover. Our landscape risk analysis revealed that areas bordering ENP function as ecological traps with certain pockets within the GEL acting as hotspots of conflict-related mortality. The majority of the conflict-related lion mortality was reported from commercial freehold farms reflecting Namibia’s colonial history and land ownership. Our findings suggest that mitigation measures to reduce human-lion conflict at the interface of ENP and other protected areas in Sub-Saharan Africa need to consider both ecological and cultural factors to have maximal impact. Given the high conflict-related mortality of lions in the landscape surrounding ENP, a conservation management strategy is critically needed to ensure human-lion coexistence within the GEL.

Abstract Invasive wild pigs (Sus scrofa) are one of the most widespread, destructive vertebrate species globally. Their success can largely be attributed to their generalist diets, which are dominated by plant material but also include diverse animal taxa. Wild pigs are demonstrated nest predators of ground-nesting birds and reptiles, and likely pose a threat to amphibians given their extensive overlap in wetland use. DNA metabarcoding of fecal samples from 222 adult wild pigs culled monthly from 2017 to 2018 revealed a diverse diet dominated by plant material, with 166 plant genera from 56 families and 18 vertebrate species identified. Diet composition varied seasonally with availability for plants and was consistent between sexes. Amphibians were the most frequent vertebrate group consumed and represented the majority of vertebrate species detected, suggesting amphibians are potentially vulnerable to predation by wild pigs in our study region. Mammal, reptile, and bird species were also detected in pig diets, but infrequently. Our results highlight the need for research on the impacts of wild pigs on amphibians to better inform management and conservation of imperiled species.

For many species, the relationship between space use and diet composition is complex, with individuals adopting varying space use strategies such as territoriality to facilitate resource acquisition. Coyotes (Canis latrans) exhibit two disparate types of space use; defending mutually exclusive territories (residents) or moving nomadically across landscapes (transients). Resident coyotes have increased access to familiar food resources, thus improved foraging opportunities to compensate for the energetic costs of defending territories. Conversely, transients do not defend territories and are able to redirect energetic costs of territorial defense towards extensive movements in search of mates and breeding opportunities. These differences in space use attributed to different behavioral strategies likely influence foraging and ultimately diet composition, but these relationships have not been well studied. We investigated diet composition of resident and transient coyotes in the southeastern United States by pairing individual space use patterns with analysis of stable carbon (δ13C) and nitrogen (δ15N) isotope values to assess diet. During 2016–2017, we monitored 41 coyotes (26 residents, 15 transients) with GPS radio-collars along the Savannah River area in the southeastern United States. We observed a canopy effect on δ13C values and little anthropogenic food in coyote diets, suggesting 13C enrichment is likely more influenced by reduced canopy cover than consumption of human foods. We also observed other land cover effects, such as agricultural cover and road density, on δ15N values as well as reduced space used by coyotes, suggesting that cover types and localized, resident-like space use can influence the degree of carnivory in coyotes. Finally, diets and niche space did not differ between resident and transient coyotes despite differences observed in the proportional contribution of potential food sources to their diets. Although our stable isotope mixing models detected differences between the diets of resident and transient coyotes, both relied mostly on mammalian prey (52.8%, SD = 15.9 for residents, 42.0%, SD = 15.6 for transients). Resident coyotes consumed more game birds (21.3%, SD = 11.6 vs 13.7%, SD = 8.8) and less fruit (10.5%, SD = 6.9 vs 21.3%, SD = 10.7) and insects (7.2%, SD = 4.7 vs 14.3%, SD = 8.5) than did transients. Our findings indicate that coyote populations fall on a feeding continuum of omnivory to carnivory in which variability in feeding strategies is influenced by land cover characteristics and space use behaviors.

The genus Dracunculus contains numerous species of subcutaneous parasites of mammals and reptiles. In North America, there are at least three mammal-infecting species of Dracunculus. Reports of Dracunculus infections have been reported from river otters (Lontra canadensis) since the early 1900s; however, little is known about the species infecting otters or their ecology. Most reports of Dracunculus do not have a definitive species identified because females, the most common sex found due to their larger size and location in the extremities of the host, lack distinguishing morphological characteristics, and few studies have used molecular methods to confirm identifications. Thus, outside of Ontario, Canada, where both D. insignis and D. lutrae have been confirmed in otters, the species of Dracunculus in river otters is unknown. In the current study, molecular characterization of nematodes from river otters revealed a high diversity of Dracunculus species. In addition to confirming D. insignis infections, two new clades were detected. One clade was a novel species in any host and the other was a clade previously detected in Virginia opossums (Didelphis virginiana) from the USA and a domestic dog from Spain. No infections with D. lutrae were detected and neither new lineage was genetically similar to D. jaguape, which was recently described from a neotropical otter (Lontra longicaudis) from Argentina. These data also indicate that Dracunculus spp. infections in otters are widespread throughout Eastern North America. Currently the life cycles for most of the Dracunculus spp. infecting otters are unknown. Studies on the diversity, life cycle, and natural history of Dracunculidae parasites in wildlife are important because the related parasite, D. medinensis (human Guinea worm) is the subject of an international eradication campaign and there are increasing reports of these parasites in new geographic locations and new hosts, including new species in humans and domestic dogs.

Understanding the epidemiology and transmission dynamics of transboundary animal diseases (TADs) among wild pigs (Sus scrofa) will aid in preventing the introduction or containment of TADs among wild populations. Given the challenges associated with studying TADs in free-ranging populations, a surrogate pathogen system may predict how pathogens may circulate and be maintained within wild free-ranging swine populations, how they may spill over into domestic populations, and how management actions may impact transmission. We assessed the suitability of Torque teno sus virus 1 (TTSuV1) to serve as a surrogate pathogen for molecular epidemiological studies in wild pigs by investigating the prevalence, persistence, correlation with host health status and genetic variability at two study areas: Archbold’s Buck Island Ranch in Florida and Savannah River Site in South Carolina. We then conducted a molecular epidemiological case study within Archbold’s Buck Island Ranch site to determine how analysis of this pathogen could inform transmission dynamics of a directly transmitted virus. Prevalence was high in both study areas (40%, n = 190), and phylogenetic analyses revealed high levels of genetic variability within and between study areas. Our case study showed that pairwise host relatedness and geographic distance were highly correlated to pairwise viral genetic similarity. Molecular epidemiological analyses revealed a distinct pattern of direct transmission from pig to pig occurring within and between family groups. Our results suggest that TTSuV1 is highly suitable for molecular epidemiological analyses and will be useful for future studies of transmission dynamics in wild free-ranging pigs.

Abstract Raccoons (Procyon lotor) are routinely translocated both legally and illegally to mitigate conflicts with humans, which has contributed to the spread of rabies virus across eastern North America. The movement behavior of translocated raccoons has important ramifications for disease transmission yet remains understudied and poorly quantified. To examine the spatial ecology of raccoons following experimental translocation, we performed reciprocal 16 km-distance translocations of 30 raccoons between habitats of high and low raccoon density (bottomland hardwood and upland pine, respectively) across the Savannah River Site (SRS) in Aiken, South Carolina, USA (2018–2019). Translocation influenced patterns of raccoon space use, with translocated animals exhibiting a 13-fold increase in 95% utilization distributions (UDs) post- compared to pre-translocation (mean 95% UD 35.8 ± 36.1 km2 vs 1.96 ± 1.17 km2). Raccoons originating from upland pine habitats consistently had greater space use and larger nightly movement distances post-translocation compared to raccoons moved from bottomland hardwood habitats, whereas these differences were generally not observed prior to translocation. Estimated home ranges of male raccoons were twice the area as estimated for female raccoons, on average, and this pattern was not affected by translocation. After a transient period lasting on average 36.5 days (SD = 30.0, range = 3.25–92.8), raccoons often resumed pre-experiment movement behavior, with 95% UD sizes not different from those prior to translocation (mean = 2.27 ± 1.63km2). Most animals established new home ranges after translocation, whereas three raccoons moved > 16 km from their release point back to the original capture location. Four animals crossed a 100-m wide river within the SRS post-translocation, but this behavior was not documented among collared raccoons prior to translocation. Large increases in space use combined with the crossing of geographic barriers such as rivers may lead to elevated contact rates with conspecifics, which can heighten disease transmission risks following translocation. These results provide additional insights regarding the potential impacts of raccoon translocation towards population level risks of rabies outbreaks and underscore the need to discourage mesocarnivore translocations to prevent further spread of wildlife rabies.

Abstract To successfully establish itself in a novel environment, an animal must make an inherent trade-off between knowledge accumulation and exploitation of knowledge gained (i.e., the exploration–exploitation dilemma). To evaluate how habitat quality affects the spatio-temporal scale of switching between exploration and exploitation during home range establishment, we conducted experimental trials comparing resource selection and space-use of translocated animals to those of reference individuals using reciprocal translocations between habitat types of differing quality. We selected wild pigs (Sus scrofa) as a model species to investigate hypotheses related to the movement behavior of translocated individuals because they are globally distributed large mammals that are often translocated within their introduced range to facilitate recreational hunting. Individuals translocated to higher quality habitat (i.e. higher proportions of bottomland hardwood habitats) exhibited smaller exploratory movements and began exploiting resources more quickly than those introduced to lower quality areas, although those in lower-quality areas demonstrated an increased rate of selection for preferred habitat as they gained knowledge of the landscape. Our data demonstrate that habitat quality mediates the spatial and temporal scale at which animals respond behaviorally to novel environments, and how these processes may determine the success of population establishment.

Due to successful conservation initiatives and legislations, the grey wolf (Canis lupus) is re-colonising its historic range in Europe. However, wolves have never been extirpated across large areas in Eastern Europe but are often constrained to remote and inaccessible places due to centuries of persecution. This study aimed to identify the potentially suitable wolf habitats in Polesia, a massive cross-border lowland region extending over southern Belarus and northern Ukraine, which are often neglected in large carnivore studies at the continental scale. We hypothesized that anthropogenic rather than environmental factors govern wolf habitat suitability. We used a dataset of 4191 GPS locations obtained from radio-collared wolves (n = 26) and confirmed observations (n = 231) during 2014–2021 and applied maximum entropy method to estimate relative habitat suitability for wolves in Polesia. Artificial light at night (ALAN), proportion of cropland and tree cover were the most important factors affecting wolf habitat suitability. Road densities contributed poorly to predicting habitat suitability for wolves. Our models predicted a quarter of Polesia as suitable habitat and revealed priority areas connecting the important source populations in the Chornobyl Exclusion Zone in the east and the Białowieża Forest in the west and thus essential for long-term wolf conservation. Our results provide the bases for effective, long-term wolf monitoring and management programs in both Belarus and Ukraine. However, national and transboundary wolf management in Polesia has been extremely challenging since 2022 due to the ongoing war and subsequent habitat degradation in this part of Europe.

Abstract The selection or avoidance of certain carrion resources by vertebrate scavengers can alter the flow of nutrients in ecosystems. Evidence suggests higher trophic level carrion is scavenged by fewer vertebrate species and persists longer when compared to lower trophic level carrion, although it is unclear how scavengers distinguish between carcasses of varying species. To investigate carnivore carrion avoidance and explore sensory recognition mechanisms in scavenging species, we investigated scavenger use of intact and altered (i.e., skin, head, and feet removed) coyote—Canis latrans (carnivore) and wild pig—Sus scrofa (omnivore) carcasses experimentally placed at the Savannah River Site, SC, USA. We predicted carnivore carcasses would persist longer due to conspecific and intraguild scavenger avoidance. Further, we hypothesized visually modifying carcasses would not reduce avoidance of carnivore carrion, given scavengers likely depend largely on chemical cues when assessing carrion resources. As expected, mammalian carnivores largely avoided scavenging on coyote carcasses, resulting in carnivore carcasses having longer depletion times than wild pig carcasses at intact and altered trials. Therefore, nutrients derived from carnivore carcasses are not as readily incorporated into higher trophic levels and scavengers largely depend on olfactory cues when assessing benefits and risks associated with varying carrion resources.

Adequate information on external exposure is often a limiting factor in ecological risk assessments because of a lack of exposure data for free-ranging animals as they interact with a heterogeneous distribution of contaminants. Instead, most wildlife exposure data are estimated from model simulations. However, empirical exposure data are rarely available to verify model results, or to test fundamental assumptions inherent in exposure assessments. We used novel, GPS-coupled radiation dosimeters on reindeer (Rangifer tarandus) to quantify their external exposure for five months in a Chernobyl fallout affected mountainous area of Norway. The exposure data were then used to test a fundamental assumption in screening-level risk assessments: Mean soil contaminant concentrations can be used to conservatively estimate chronic external exposures to representative animals. External exposure and GPS coordinates were obtained hourly and compared to model simulations of exposure based on airborne surveys of 137Cs soil contamination. Our objectives were to determine 1) if modelled dose estimates based on mean 137Cs soil concentrations were conservative, and 2) if modelled dose estimates could be improved by adjusting for spatial–temporal heterogeneity of contaminants using 137Cs concentrations within reindeers’ GPS-defined home ranges and core areas. We found that external exposure modelled from mean soil contaminant concentrations underestimated exposure by 70% compared to empirical measurements from GPS-dosimeter collars worn on individual animals. Using area-weighted mean contaminant levels within the animals’ home range improved the model estimates, but still underestimated the field dosimeter data by 53%. The best estimate of external exposure was obtained when high-use areas within their home range, along with spatial heterogeneity of the contaminant, was accounted for by using area-weighted mean soil contaminant levels within GPS-derived core areas. Accounting for temporal use of core areas within animals’ home range provided a 24% improvement. A comparison of our analyses to similar work performed on Chernobyl wolves found the two studies concur that risk assessors should be cautious in relying on the paradigm of using averaged soil contaminant levels to provide conservative estimates of external exposure to representative individuals. Results of modelled external exposure based on mean soil contaminant concentrations could misguide assessors and cause inappropriate management decisions.

Large amounts of Cesium-137 (137Cs, physical half-life ca. 30 years) were released into the environment from the 2011 Fukushima Daiichi Nuclear Power Plant (FDNPP) accident in Japan. Initially contaminants from the accident were deposited on the surface soil layers in terrestrial ecosystems, but over time vertical migration of 137Cs has occurred, with most 137Cs now distributed a few centimeters below the soil surface. Wild boar (Sus scrofa) rooting likely alters the distribution of contaminants in soil profiles. Such impacts may be particularly pronounced in areas contaminated from the FDNPP accident, given the increase in abundance of wild boar in these areas. Our objective was to determine whether rooting by wild boar alters the distribution of 137Cs contamination within soil profiles through collection of soil cores from control sites and two different types of rooting samples, “new depressions” and “old depressions” within the difficult-to-return zone surrounding the FDNPP. The 137Cs ratio [activity concentration of 137Cs at each depth / the maximum activity concentration of 137Cs in each core sample] rapidly decreased after reaching a peak at the control sites. However, the 137Cs ratio at all rooted sites decreased more slowly, and 137Cs was distributed deeper into the soil layers than control sites, across both new and old rooting sites. Moreover, the 137Cs soil profiles varied greatly among core samples and sampling sites. These results demonstrate that soil disturbance from wild boar rooting can directly influence the distribution of contaminants within soil profiles. However, the extent to which deeper layers are affected by contamination depends on the unique characteristics (i.e., depth of rooting) of each wild boar rooting disturbance. Further studies are needed to examine the effects of long-term rooting on the alteration of 137Cs in soil profiles.

Abstract The 2011 nuclear accident in Fukushima, Japan caused the evacuation of > 100,000 people and prompted studies on environmental impacts of radiological contamination. However, few researchers have explored how the human evacuation has affected ecosystem processes. Despite contamination, one common scavenger (wild boar, Sus scrofa) is 2–3× more abundant inside the Fukushima Exclusion Zone (FEZ). Shifts in abundance of some scavenger species can have cascading effects on ecosystems, so our objective was to investigate impacts of the evacuation and the resulting increase in wild boar on vertebrate scavenger communities. We deployed cameras at 300 carcasses in the FEZ and a nearby inhabited area, and quantified carcass fate, scavenger species, and detection/persistence times. We also tested effects of carcass size and habitat on scavenger community composition and efficiency by balancing trials across two carcass sizes and habitats in each zone. Overall scavenger richness and carcass removal rates (73%) were similar in the FEZ and inhabited area, but species-specific carcass removal rates and occurrence differed between zones. Wild boar removed substantially more carcasses inside the FEZ, with implications for nutrient and contaminant distribution. Our results suggest carcass size affects scavenging dynamics more than human activity or habitat, and abundance changes of common scavengers can influence carrion resource allocation.

The erection of peripheral fences around protected areas has been shown to adversely affect the movement behavior, genetic connectivity and long-term viability of animal populations. However, fencing is also used extensively to reduce human encroachment, limit poaching activities, and mitigate human–wildlife conflicts. Limited information on the movement behavior of carnivores is available along the periphery of protected areas, particularly in semi-arid environments. To quantify the potential effects of the 824 km perimeter fence surrounding the Etosha National Park (Etosha, Namibia) on the movement and space use of carnivores, we collected GPS movement data from 36 lions (Panthera leo) and seven spotted hyenas (Crocuta crocuta) over a 14-year period. For these two apex carnivores, we measured the impact of the Etosha fence on their movements (step lengths and path straightness), calculated the likelihood of fence crossings, and evaluated the spatial–temporal characteristics of these crossings. Our results indicated that lions and hyenas moved faster (longer step lengths) when closer to the fence. Lions showed no variation in path straightness with respect to the fence but hyenas had straighter movement paths when closer to the fence. When moving within the vicinity of the fence, lions had a 9% likelihood of crossing the fence, while hyenas were much more likely (18%) to do so, which suggests that the Etosha fence was more permeable for hyenas than for lions. Fence crossings predominantly occurred at night for both species. Lions were more likely to cross the fence during the cold dry season (May–August) whereas hyenas crossed more often during the warm wet season (December–April). Female and adult lions had a higher probability of crossing the fence than males and subadults. These findings could be useful in the development of appropriate strategies to mitigate human–carnivore conflicts and thus promote the conservation of carnivores in multiple-use areas along the periphery of the park. Further research is needed to better elucidate the impact of perimeter fences on a broader suite of carnivores and other large mammals, including species-specific variation in the response to fences, as well as the economic benefits of fencing provided to local communities.

Abstract Skewed sex ratios at birth are widely reported in wild populations, however, the extent to which parents are able to modulate the sex ratio of offspring to maximize their own fitness remains unclear. This is particularly true for highly polytocous species as maximizing fitness may include trade‐offs between sex ratio and the size and number of offspring in litters. In such cases, it may be adaptive for mothers to adjust both the number of offspring per litter and offspring sex to maximize individual fitness. Investigating maternal sex allocation in wild pigs (Sus scrofa) under stochastic environmental conditions, we predicted that under favorable conditions, high‐quality mothers (larger and older) would produce male‐biased litters and invest more in producing larger litters with more males. We also predicted sex ratio would vary relative to litter size, with a male‐bias among smaller litters. We found evidence that increasing wild boar ancestry, maternal age and condition, and resource availability may weakly contribute to male‐biased sex ratio, however, unknown factors not measured in this study are assumed to be more influential. High‐quality mothers allocated more resources to litter production, but this relationship was driven by adjustment of litter size, not sex ratio. There was no relationship between sex ratio and litter size. Collectively, our results emphasized that adjustment of litter size appeared to be the primary reproductive characteristic manipulated in wild pigs to increase fitness rather than adjustment of offspring sex ratio.

Abstract Interest in control methods for invasive wild pigs (Sus scrofa) has increased due to their range expansion, population growth, and an improved understanding of their destructive ecological and economic effects. Recent technological advances in traps for control of pig populations facilitate capture of entire social groups (sounders), but the efficacy of “whole-sounder” trapping strategies is heavily dependent on the degree of territoriality among sounders, a topic little research has explored. We assessed territoriality in wild pig sounders on the Savannah River Site, South Carolina, USA, and examined whether availability of food resources provided by a municipal-waste landfill affected among-sounder territoriality. We estimated utilization distribution overlap and dynamic interactions among 18 neighboring sounders around a landfill. We found that although neighboring sounders overlapped in space, intensity of use in shared areas was uniformly low, indicating territorial behavior. Neighbors tended to share slightly more space when closer to the landfill waste cells, indicating availability of a super-abundant resource somewhat weakens the degree of territoriality among sounders. Nevertheless, we conclude that sounders behaved in a generally territorial manner, and we discuss implications for whole-sounder trapping programs, particularly near concentrated resources such as landfills and crop fields.

Abstract Understanding factors influencing survival of neonates for wild species is important for successful management, particularly for determining drivers of population dynamics. Wild pigs (Sus scrofa) are invasive and populations are rapidly increasing in part due to high reproductive capacity. Survival of adults is generally high, however, survival of piglets, and particularly neonates, is largely unknown. We located neonates at the natal nest and quantified survival in relation to individual and maternal biological attributes, and environmental variables. During 2017–2020, we captured 50 neonates from 13 litters and documented 28 mortalities (56%) over six weeks. Survival was positively influenced by pelage coloration, likely as a form of camouflage from predators. Male neonates had higher survival. They were born larger than females, which could be beneficial for thermoregulation and competition for milk. Neonates born to larger sows had lower survival. Sow size was positively correlated with litter size, and this finding may reflect the increased nutritional demands of sustaining large litters, or difficulties in defending more neonates against predators. Neonates born in warmer months had higher survival than those born in cooler months. Neonates are inefficient thermoregulators, thus being born in warmer months could be beneficial for maintaining homeostasis as well as access to more food resources. These are the largest and most complete data for neonate wild pig survival and will inform population models for the development of management strategies to reduce negative impacts of this destructive invasive species on native ecosystems.

Abstract Human altered landscapes have caused declines in the diversity of wildlife where behaviorally plastic species (i.e., mesocarnivores and invasive species) tend to monopolize these areas and consume predictable and readily accessible food resources, such as human food waste and carrion. Increased consumption of carrion by vertebrates and invasive invertebrate species can alter population dynamics of native necrophagous insects relying on these resources. We tested the hypothesis that vertebrate scavengers and invasive species reduce blow fly (1) ability to use carrion and (2) reproduction in human‐impacted environments in central Texas, USA, with season, habitat (field and wooded landscapes), and carrion type (species of carrion and coat color) acting synergistically. Vertebrate scavengers in this habitat, of which 75% of the documented species were mesocarnivores and obligate scavengers, consumed 100% of carrion during the winter and 62% during summer despite having low species richness (2–5 species). Of the remaining carcasses available for arthropod activity during summer, the invasive red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae), monopolized 34%, and blow flies (e.g., Lucilia eximia and Chrysomya rufifacies [Diptera: Calliphoridae]) were only able to colonize 25%. Approximately 90% of carrion that was utilized by blow flies was co‐colonized by fire ants, and subsequent production of adult blow flies experienced up to a ninefold reduction in production compared with carcasses that were not scavenged by vertebrates or fire ants. Our results demonstrate oviposition resources used by blow flies in environments altered by human activity are reduced significantly by vertebrate scavengers and an invasive ant species. Future research should determine whether competitive interactions between vertebrate and invasive ant competitors for access to carrion resources have population‐level impacts to blow flies in human‐mediated ecosystems, or whether blow flies are able to shift to other resources to maintain sustainable populations and continue providing ecosystem services, such as pollination.

Abstract Scavenging plays a vital role in maintaining ecosystem health and contributing to ecological functions; however, research in this sub‐discipline of ecology is underutilized in developing and implementing wildlife conservation and management strategies. We provide an examination of the literature and recommend priorities for research where improved understanding of scavenging dynamics can facilitate the development and refinement of applied wildlife conservation and management strategies. Due to the application of scavenging research broadly within ecology, scavenging studies should be implemented for informing management decisions. In particular, a more direct link should be established between scavenging dynamics and applied management programs related to informing pharmaceutical delivery and population control through bait uptake for scavenging species, prevention of unintentional poisoning of nontarget scavenging species, the epidemiological role that scavenging species play in disease dynamics, estimating wildlife mortalities, nutrient transfer facilitated by scavenging activity, and conservation of imperiled facultative scavenging species. This commentary is intended to provide information on the paucity of data in scavenging research and present recommendations for further studies that can inform decisions in wildlife conservation and management. Additionally, we provide a framework for decision‐making when determining how to apply scavenging ecology research for management practices and policies. Due to the implications that scavenging species have on ecosystem health, and their overall global decline as a result of anthropic activities, it is imperative to advance studies in the field of scavenging ecology that can inform applied conservation and management programs.

Abstract Elucidating correlations between wild pig (Sus scrofa) behavior and landscape attributes can aid in the advancement of management strategies for controlling populations. Using GPS data from 49 wild pigs in the southeastern U.S., we used hidden Markov models to define movement path characteristics and assign behaviors (e.g., resting, foraging, travelling). We then explored the connection between these behaviors and resource selection for both sexes between two distinct seasons based on forage availability (i.e., low forage, high forage). Females demonstrated a crepuscular activity pattern in the high-forage season and a variable pattern in the low-forage season, while males exhibited nocturnal activity patterns across both seasons. Wild pigs selected for bottomland hardwoods and dense canopy cover in all behavioral states in both seasons. Males selected for diversity in vegetation types while foraging in the low-forage season compared to the high-forage season and demonstrated an increased use of linear anthropogenic features across seasons while traveling. Wild pigs can establish populations and home ranges in an array of landscapes, but our results demonstrate male and female pigs exhibit clear differences in movement behavior and there are key resources associated with common behaviors that can be targeted to improve the efficiency of management programs.

Abstract With a rise in human induced changes to natural habitats, large predators are forced to share space and resources with people to coexist within multiple-use landscapes. Within such shared landscapes, co-occurrence of humans and predators often leads to human-carnivore conflicts and pose a substantial challenge for biodiversity conservation. To better elucidate large carnivore space use within a hotspot of human-wildlife conflicts, we used GPS data for leopards (N = 6) to identify behavioral states and document spatial patterns of resource selection in response to season and human activity periods within a fragmented landscape of North Bengal, eastern India. We identified two major behavioral states (i.e. resting and travelling). From the resource selection models, we found leopards selected habitats with dense to moderate vegetation cover and proximity to water while resting and travelling within the landscape. During the dry season, when risk of human-leopard conflicts is highest, leopards selected tea plantations, forest patches but avoided protected areas. These results suggest a potential for increase in human-carnivore conflicts and a strategy to conserve large predators within multiple-use landscapes of South Asia.

The present study is aimed at evaluating the genetic diversity, genetic status and the extent of hybridization with the domestic horse for the Przhevalski’s horse (Equus ferus przewalskii Poliakov 1881) population free-ranging in the territory of the Chernobyl Exclusion Zone (CEZ) in Belarus and Ukraine. The sample size included 12 individuals (10 sampled in the Belarusian part of the CEZ and 2 from the Ukrainian part of the CEZ). Ten microsatellites recommended by the International Society for Animal Genetics (ISAG) for horse genetic status and pedigree determination were used as markers in this study. The fragment analysis data obtained utilising this microsatellite panel determined that two individuals from Belarus possess no allelic variants typical for Przhevalski’s horse. Most of the other individuals presented diagnostically valuable allelic variants. Demographic history analysis for the population did not indicate any drastic population shrinkage events in the population’s recent history. The studied population is characterised by heterogeneous population structure with signs of inbreeding (0.21 %), intermediate level of genetic diversity (He = 0.63) and allelic richness (5.15), possesses 16 unique alleles among 2 microsatellite loci and valuable alleles for loci HMS3 and HMS7 (46.4 and 67.9 % specific alleles for Przhevalski’s horse, respectively). Genetic structure evaluation for the population was performed via Bayesian population structure analysis and factorial correspondence analysis (FCA), which indicated the presence of intrapopulation genetic subdivision. Taking into account the obtained indicators of genetic diversity, we may conclude on the relatively favourable status of Przewalski’s horse in the exclusion zone with good potential for the long-term existence of the species population in the wild. In order to minimise inbreeding effects and the risk of a decline in genetic diversity in the population of Przewalski’s horse of the exclusion zone, as well as to increase the value of this free-living group to preserve the gene pool of the species as a whole, it is necessary to provide detailed genetic monitoring of the livestock’s state, as well as develop a regional population management plan, including measures aimed to minimise the possibility of further hybridisation of wild horses with domestic ones.

Abstract Motion‐activated wildlife cameras (or “camera traps”) are frequently used to remotely and noninvasively observe animals. The vast number of images collected from camera trap projects has prompted some biologists to employ machine learning algorithms to automatically recognize species in these images, or at least filter‐out images that do not contain animals. These approaches are often limited by model transferability, as a model trained to recognize species from one location might not work as well for the same species in different locations. Furthermore, these methods often require advanced computational skills, making them inaccessible to many biologists. We used 3 million camera trap images from 18 studies in 10 states across the United States of America to train two deep neural networks, one that recognizes 58 species, the “species model,” and one that determines if an image is empty or if it contains an animal, the “empty‐animal model.” Our species model and empty‐animal model had accuracies of 96.8% and 97.3%, respectively. Furthermore, the models performed well on some out‐of‐sample datasets, as the species model had 91% accuracy on species from Canada (accuracy range 36%–91% across all out‐of‐sample datasets) and the empty‐animal model achieved an accuracy of 91%–94% on out‐of‐sample datasets from different continents. Our software addresses some of the limitations of using machine learning to classify images from camera traps. By including many species from several locations, our species model is potentially applicable to many camera trap studies in North America. We also found that our empty‐animal model can facilitate removal of images without animals globally. We provide the trained models in an R package (MLWIC2: Machine Learning for Wildlife Image Classification in R), which contains Shiny Applications that allow scientists with minimal programming experience to use trained models and train new models in six neural network architectures with varying depths.

Summary Contaminants such as heavy metals may contribute to the dissemination of antimicrobial resistance (AMR) by enriching resistance gene determinants via co‐selection mechanisms. In the present study, a survey was performed on soils collected from four areas at the Savannah River Site (SRS), South Carolina, USA, with varying contaminant profiles: relatively pristine (Upper Three Runs), heavy metals (Ash Basins), radionuclides (Pond B) and heavy metal and radionuclides (Tim’s Branch). Using 16S rRNA gene amplicon sequencing, we explored the structure and diversity of soil bacterial communities. Sites with legacies of metal and/or radionuclide contamination displayed significantly lower bacterial diversity compared to the reference site. Metagenomic analysis indicated that multidrug and vancomycin antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) including those associated with copper, arsenic, iron, nickel and zinc were prominent in all soils including the reference site. However, significant differences were found in the relative abundance and diversity of certain ARGs and MRGs in soils with metal/radionuclide contaminated soils compared to the reference site. Co‐occurrence patterns revealed significant ARG/MRG subtypes in predominant soil taxa including Acidobacteriaceae, Bradyrhizobium, Mycobacterium, Streptomyces, Verrumicrobium, Actinomadura and Solirubacterales. Overall, the study emphasizes the potential risk of human activities on the dissemination of AMR in the environment.

ABSTRACT Baiting is a fundamental strategy for the global management of wild pigs (Sus scrofa); however, little information exists on how anthropogenic bait affects wild pig movements on a landscape. We investigated factors that are important in determining the spatial area of attraction for wild pigs to bait (‘area of influence’ of a bait site) using data from Global Positioning System (GPS) collars and locations of bait sites. We monitored movements of wild pigs in 2 distinct study areas in the United States from February to September 2016 and used locational data using GPS collars to analyze the influence of habitat quality (dependent on site), home range size, number of bait sites in the home range, distance to a bait site, and sex in relation to movement in time and space. We determined the average area of influence by calculating the area of a circle with the radius as the average maximum distance travelled by wild pigs to reach a bait site. The average area of influence for our bait sites was 6.7 km2 (or a radius of approximately 1.5 km), suggesting a bait spacing of approximately 1.5 km would be adequate to capture visitation by most wild pigs and a spacing of 3 km could allow substantial visitation while minimizing redundant effort depending on the spatial structure of the populations. Eighty percent of wild pigs first visited bait sites within 8.9 days after bait deployment; and they visited earlier when their home range size was larger. As the number of bait sites in an individual's home range increased, individual pigs visited more bait sites, and the probability of a visit increased dramatically up to approximately 5 bait sites and much less thereafter. Wild pigs travelled farther distances to visit bait sites in lower quality habitat. Our results support the hypothesis that habitat quality can mediate the efficacy of baiting programs for wildlife by influencing their movement patterns and motivation to use anthropogenic resources. Our results suggest wild pigs will travel extensively within their home range to visit bait sites, and that in lower quality habitat, most animals will find bait sites more quickly. Determining the area of influence of bait sites can increase the efficacy of planning and monitoring management programs. Our study provides new information to help managers plan baiting designs to attract the greatest number of pigs. © 2020 The Wildlife Society.

ABSTRACT Reliable and efficient population estimates are a critical need for effective management of invasive wild pigs (Sus scrofa). We evaluated the use of 10‐day camera grids for rapid population assessment (RPA) of wild pigs at 3 study sites that varied in vegetation communities and wild pig densities. Study areas included Buck Island Ranch, Florida; Tejon Ranch, California; and the Savannah River Site, South Carolina, USA, during 2016–2018. Rapid population assessments grids were composed of baited camera traps spaced approximately 500 or 750 m apart. Two RPA grids were deployed per study site and each grid was deployed twice (4–6 months apart) to assess changes in response to season or population control efforts. We assessed the ability of RPA grids to track population trends, how camera number influenced estimate precision, and how relative abundance indices related to density estimates. We detected changes in occupancy probability, detection probability, and N‐mixture estimates following removal operations and between seasons, but the ability of RPA grids to track population trends was dependent on the statistical method used and number of cameras traps. Increasing the number of cameras traps used in RPA grids increased precision, and these results can be used in determining survey design and estimate choice. We found that estimates of occupancy probability, detection probability, and N‐mixture estimates were positively correlated with spatially explicit capture–recapture density estimates. Thus, these less labor‐intensive estimates from RPA grids showed potential to index the relative abundance of wild pigs in some systems. Our evaluation of RPAs indicates that using study‐specific combinations of statistical method and number of cameras can provide a useful tool for monitoring wild pig presence, tracking population trends, and evaluating the effectiveness of management actions. © 2020 The Wildlife Society.

The health effects associated with chronic low-dose, low-dose rate (LD-LDR) exposures to environmental radiation are uncertain. All dose-effect studies conducted outside controlled laboratory conditions are challenged by inherent complexities of ecological systems and difficulties quantifying dose to free-ranging organisms in natural environments. Consequently, the effects of chronic LD-LDR radiation exposures on wildlife health remain poorly understood and much debated. Here, samples from wild boar (Sus scrofa leucomystax) and rat snakes (Elaphe spp.) were collected between 2016 and 2018 across a gradient of radiation exposures in Fukushima, Japan. In vivo biomarkers of DNA damage and stress were evaluated as a function of multiple measurements of radiation dose. Specifically, we assessed frequencies of dicentric chromosomes (Telomere-Centromere Fluorescence in situ Hybridization: TC-FISH), telomere length (Telo-FISH, qPCR), and cortisol hormone levels (Enzyme Immunoassay: EIA) in wild boar, and telomere length (qPCR) in snakes. These biological parameters were then correlated to robust calculations of radiation dose rate at the time of capture and plausible upper bound lifetime dose, both of which incorporated internal and external dose. No significant relationships were observed between dicentric chromosome frequencies or telomere length and dose rate at capture or lifetime dose (p value range: 0.20–0.97). Radiation exposure significantly associated only with cortisol, where lower concentrations were associated with higher dose rates (r2 = 0.58; p

Abstract Noninvasive, camera‐based, scent station surveys have become a common approach to estimate population parameters for mammalian carnivores. Many carnivores are particularly sensitive to olfactory cues; and, as such, the quality and quantity of odor attractant used during surveys can significantly affect the accuracy and precision of the survey. Additionally, survey length is also an important consideration because many mammalian predators are highly mobile and elusive in nature. Using scent station surveys in autumn 2013 and 2014 on the Savannah River Site, Aiken, South Carolina, USA, we tested the effects of 5 lures: fatty acid, fish oil, synthetic fermented egg, beaver castor, and skunk essence on visitation rates, visitation probability, and latency to visitation of multiple predator species. We also estimated occupancy probability for 2 carnivore species using different survey lengths to determine the effect of survey period on the precision of occupancy estimates. We found that skunk essence had greater visitation probability and visits than other lures evaluated. Species‐specific bait‐preference models revealed that raccoons (Procyon lotor) were more likely to visit skunk essence, and coyotes (Canis latrans) were less likely to visit fish oil as compared with fatty acid. Latency to visitation ranged from 104.7 to 112.1 hours and did not vary among scents. Longer survey durations resulted in more precise estimates of occupancy and detection probability. Our results suggest a wide range of scents commonly used for carnivore capture may perform as well as, or better than, fatty acid for noninvasive carnivore surveys. Further, study designs should carefully consider the duration of scent‐station surveys depending on the level of precision desired in estimates of occupancy and detection probability. © 2019 The Wildlife Society.

Abstract Background As obligate scavengers utilizing similar habitats, interspecific competition undoubtedly occurs between resident black (Coragyps atratus) and turkey (Cathartes aura) vultures. In the interest of exploring how sympatric species coexist through habitat segregation, we examined resource selection of resident black and turkey vultures in the southeastern United States (US) for evidence of niche differentiation. Methods Using fine-scale movement data, we assessed interspecific seasonal differences in monthly roost reuse frequency and roost site fidelity, as well as monthly flight, roost, and diurnal rest site resource selection based on > 2.8 million locations of 9 black vultures and 9 turkey vultures tracked from September 2013 to August 2015 using Groupe Spécial Mobile/Global Positioning System (GSM/GPS) transmitters. Results Black vultures generally exhibited greater roost fidelity as well as a greater maximum number of nights spent at a single roost than turkey vultures. Patterns of flight, roost, and resting habitat selection within the home range varied monthly as well as between species, providing evidence for habitat segregation and niche differentiation by sympatric vultures. In particular, our results indicate the importance of wooded wetlands for resting and roosting locations for both species, and revealed clear differences in the use of forested habitats between species during flight, resting, and roosting behavioral states. Conclusions By examining differences in resource selection and spatial ecology of black and turkey vultures across a range of behaviors, this study demonstrates mechanisms of niche differentiation in these ecologically similar species, and enhances potential for conservation and informed management of this important group of birds.

The order Piroplasmida contains a diverse group of intracellular parasites, many of which can cause significant disease in humans, domestic animals, and wildlife. Two piroplasm species have been reported from raccoons (Procyon lotor), Babesia lotori (Babesia sensu stricto clade) and a species related to Babesia microti (called B. microti-like sp.). The goal of this study was to investigate prevalence, distribution, and diversity of Babesia in raccoons. We tested raccoons from selected regions in the United States and Canada for the presence of Babesia sensu stricto and Babesia microti-like sp. piroplasms. Infections of Babesia microti-like sp. were found in nearly all locations sampled, often with high prevalence, while Babesia sensu stricto infections had higher prevalence in the Southeastern United States (20–45% prevalence). Co-infections with both Babesia sp. were common. Sequencing of the partial 18S rRNA and cytochrome oxidase subunit 1 (cox1) genes led to the discovery of two new Babesia species, both found in several locations in the eastern and western United States. One novel Babesia sensu stricto sp. was most similar to Babesia gibsoni while the other Babesia species was present in the ‘western piroplasm’ group and was related to Babesia conradae. Phylogenetic analysis of the cox1 sequences indicated possible eastern and western genetic variants for the three Babesia sensu stricto species. Additional analyses are needed to characterize these novel species; however, this study indicates there are now at least four species of piroplasms infecting raccoons in the United States and Canada (Babesia microti-like sp., Babesia lotori, a novel Babesia sensu stricto sp., a novel western Babesia sp.) and a possible fifth species (Babesia sensu stricto) in raccoons in Japan. Keywords: Babesia, Cryptic species, Piroplasms, Raccoons, Tick-borne pathogens

The aquatic cooling canal system associated with a nuclear reactor built in the early 1950s received accidental releases of radiocesium (137Cs) from the reactor between 1954 and 1964, resulting in the dispersion of ~8.2 × 1012 Bq of 137Cs into the associated canals and ponds. The primary purpose of this study was to document concentrations of 137Cs in littoral zone fish currently occupying components of the cooling canal system, 3 canals and 2 impoundments, to determine how concentrations varied among these various components. Secondarily, we examined for potential influence of weirs within the canal system on concentrations in fish as well as the potential relationship between fish species and body size and on 137Cs concentrations in fish. We collected samples of sediment, biofilm, and fish from each component of the R-Reactor cooling system and compared 137Cs among sites and species in individual sites. Concentrations of 137Cs in sediments, biofilms and mosquitofish varied significantly among sampling areas with higher concentrations in RCAN1, a canal segment that was the closest to the reactor and received reactor effluent for a longer period than other components. Comparisons among other components of the cooling system, and species comparisons relative to presumed trophic positions and fish length were not consistent. However, littoral zone fish in the cooling canal system continue to bioaccumulate 137Cs >50 years after the original releases of contamination. Keywords: 137Cs, Radiocesium, Fish, Reactor cooling canals, Bioaccumulation

ABSTRACT The prevalence of antibiotic resistance genes (ARGs) can be driven by direct selection from antibiotic use and indirect selection from substances such as heavy metals (HMs). While significant progress has been made to characterize the influence of HMs on the enrichment and dissemination of ARGs in the environment, there is still much we do not know. To fill this knowledge gap, we present a comprehensive analysis of gut bacteria associated with wild cotton mice (Peromyscus gossypinus) trapped from several areas affected by legacies of HM and radionuclide contamination. We explore how these contaminants affect gut microbial community (GMC) composition and diversity and the enrichment of antibiotic, biocide, and metal resistance genes. Although we were able to identify that a myriad of co-occurring antimicrobial and HM resistance genes appear in mice from all areas, including those without a history of contamination, the proportions of co-occurring ARGs and metal resistance genes (MRGs) are higher in sites with radionuclide contamination. These results support those from several previous studies and enhance our understanding of the coselection process, while providing new insights into the ubiquity of antimicrobial resistance in the resistome of wild animals. IMPORTANCE Antimicrobial resistance is a serious global public health concern because of its prevalence and ubiquitous distribution. The rapid dissemination of antibiotic resistance genes is thought to be the result of the massive overuse of antibiotics in agriculture and therapeutics. However, previous studies have demonstrated that the spread of antibiotic resistance genes can also be influenced by heavy metal contamination. This coselection phenomenon, whereby different resistance determinants are genetically linked on the same genetic element (coresistance) or a single genetic element provides resistance to multiple antimicrobial agents (cross-resistance), has profound clinical and environmental implications. In contrast to antibiotics, heavy metals can persist in the environment as a selection pressure for long periods of time. Thus, it is important to understand how antibiotic resistance genes are distributed in the environment and to what extent heavy metal contaminants may be driving their selection, which we have done in one environmental setting.

Abstract Background New World vultures (Cathartiformes: Cathartidae) are obligate scavengers comprised of seven species in five genera throughout the Americas. Of these, turkey vultures (Cathartes aura) and black vultures (Coragyps atratus) are the most widespread and, although ecologically similar, have evolved differences in morphology, physiology, and behaviour. Three species of haemosporidians have been reported in New World vultures to date: Haemoproteus catharti, Leucocytozoon toddi and Plasmodium elongatum, although few studies have investigated haemosporidian parasites in this important group of species. In this study, morphological and molecular methods were used to investigate the epidemiology and molecular biology of haemosporidian parasites of New World vultures in North America. Methods Blood and/or tissue samples were obtained from 162 turkey vultures and 95 black vultures in six states of the USA. Parasites were identified based on their morphology in blood smears, and sequences of the mitochondrial cytochrome b and nuclear adenylosuccinate lyase genes were obtained for molecular characterization. Results No parasites were detected in black vultures, whereas 24% of turkey vultures across all sampling locations were positive for H. catharti by blood smear analysis and/or PCR testing. The phylogenetic analysis of cytochrome b gene sequences revealed that H. catharti is closely related to MYCAMH1, a yet unidentified haemosporidian from wood storks (Mycteria americana) in southeastern USA and northern Brazil. Haemoproteus catharti and MYCAMH1 represent a clade that is unmistakably separate from all other Haemoproteus spp., being most closely related to Haemocystidium spp. from reptiles and to Plasmodium spp. from birds and reptiles. Conclusions Haemoproteus catharti is a widely-distributed parasite of turkey vultures in North America that is evolutionarily distinct from other haemosporidian parasites. These results reveal that the genetic diversity and evolutionary relationships of avian haemosporidians are still being uncovered, and future studies combining a comprehensive evaluation of morphological and life cycle characteristics with the analysis of multiple nuclear and mitochondrial genes will be useful to redefine the genus boundaries of these parasites and to re-evaluate the relationships amongst haemosporidians of birds, reptiles and mammals.

ABSTRACT As a result of substantial ecological and economic damage attributed to wild pigs (Sus scrofa), there is international interest in using pharmaceutical baits to control populations. To assess the efficacy and specificity of baiting programs, chemical biomarkers can be used to evaluate uptake of pharmaceutical baits. Rhodamine B (RB) is known to be an effective biomarker in wild pigs. However, significant data gaps exist regarding the minimum effective dosage and persistence of RB in wild pigs. We used a controlled double‐blind study experiment conducted in spring of 2014 on the Savannah River Site, Aiken, South Carolina, USA, wherein we administered a one‐time dose of RB at 3 treatment levels (5 mg/kg, 15 mg/kg, or 30 mg/kg) to 15 captive pigs, with 5 pigs/treatment group to investigate persistence of RB. Facial vibrissae were collected pre‐RB ingestion as a control and every 2 weeks post‐RB ingestion for 12 weeks. We examined samples for RB presence and used a generalized linear mixed model (GLMM) to determine the influence of treatment dose on persistence of RB. Additionally, we measured distance moved by the RB mark away from the vibrissae root and used a GLMM to assess movement rates of RB bands along growing vibrissae. We found consistently greater persistence of RB in the 15‐ and 30‐mg/kg treatments across the sampling period. A significant, positive movement trend in RB bands was observed within the 15 mg/kg and 30 mg/kg groups. Based on our results, a 15 mg/kg dosage can be considered a minimum effective dose for wild pigs and will reliably produce a detectable RB mark up to and likely beyond 12 weeks after ingestion. © 2017 This article is a U.S. Government work and is in the public domain in the USA.

ABSTRACT Bird strikes are a major safety and financial concern for modern aviation. Audible stimuli are common bird dispersal techniques, but their effectiveness is limited by the saliency and relevance of the stimulus. Furthermore, high ambient sound levels present at airfields might require that effective audible stimuli rely more on total volume (i.e., exceeding physiological tolerances) than ecological relevance. Acoustic hailing devices (AHD) are capable of sound output with a narrow beam width and at volumes high enough to cause physical discomfort at long distances. We tested the effectiveness of an AHD as a dispersal tool on free‐ranging birds recognized as hazardous to aviation safety at the Savannah River Site and Phinizy Swamp Nature Park in South Carolina and Georgia, USA, respectively, between October 2013 and March 2015. Our study design included experimental trials with timed‐interval counts of birds directly before and after AHD treatment. For most species, counts of birds associated with treatment periods (use of AHD) and control periods (no use of AHD) occurred on different days. Sound treatments yielded variable success at dispersing birds. Specifically, AHD treatment was effective for dispersing vultures (Coragyps atratus and Cathartes aura) and gulls (Laridae), but ineffective for dispersing blackbirds (Icteridae), diving ducks (Aythya spp., Bucephala spp., Oxyura spp.), and coots (Fulica americana). Trials were conducted in a relatively quiet environment with birds that were unhabituated to excessive noise; thus, we cannot unequivocally recommend an AHD as a universally effective avian dispersing tool. However, future research should consider AHD testing integrated with other methods, as well as investigation of treatments that might be salient to specific target species. © 2017 The Wildlife Society.

Abstract Background The movement behavior of an animal is determined by extrinsic and intrinsic factors that operate at multiple spatio-temporal scales, yet much of our knowledge of animal movement comes from studies that examine only one or two scales concurrently. Understanding the drivers of animal movement across multiple scales is crucial for understanding the fundamentals of movement ecology, predicting changes in distribution, describing disease dynamics, and identifying efficient methods of wildlife conservation and management. Methods We obtained over 400,000 GPS locations of wild pigs from 13 different studies spanning six states in southern U.S.A., and quantified movement rates and home range size within a single analytical framework. We used a generalized additive mixed model framework to quantify the effects of five broad predictor categories on movement: individual-level attributes, geographic factors, landscape attributes, meteorological conditions, and temporal variables. We examined effects of predictors across three temporal scales: daily, monthly, and using all data during the study period. We considered both local environmental factors such as daily weather data and distance to various resources on the landscape, as well as factors acting at a broader spatial scale such as ecoregion and season. Results We found meteorological variables (temperature and pressure), landscape features (distance to water sources), a broad-scale geographic factor (ecoregion), and individual-level characteristics (sex-age class), drove wild pig movement across all scales, but both the magnitude and shape of covariate relationships to movement differed across temporal scales. Conclusions The analytical framework we present can be used to assess movement patterns arising from multiple data sources for a range of species while accounting for spatio-temporal correlations. Our analyses show the magnitude by which reaction norms can change based on the temporal scale of response data, illustrating the importance of appropriately defining temporal scales of both the movement response and covariates depending on the intended implications of research (e.g., predicting effects of movement due to climate change versus planning local-scale management). We argue that consideration of multiple spatial scales within the same framework (rather than comparing across separate studies post-hoc) gives a more accurate quantification of cross-scale spatial effects by appropriately accounting for error correlation.

Measurements of external contaminant exposures on individual wildlife are rare because of difficulties in using contaminant monitors on free-ranging animals. Most wildlife contaminant exposure data are therefore simulated with computer models. Rarely are empirical exposure data available to verify model simulations, or to test fundamental assumptions inherent in exposure assessments. We used GPS-coupled contaminant monitors to quantify external exposures to individual wolves (Canis lupus) living within the Belarus portion of Chernobyl's 30-km exclusion zone. The study provided data on animal location and contaminant exposure every 35 min for 6 months, resulting in ~6600 individual locations and 137Cs external exposure readings per wolf, representing the most robust external exposure data published to date on free ranging animals. The data provided information on variation in external exposure for each animal over time, as well as variation in external exposure among the eight wolves across the landscape of Chernobyl. The exposure data were then used to test a fundamental assumption in screening-level risk assessments, espoused in guidance documents of the U.S. Environmental Protection Agency and U.S. Department of Energy, — Mean contaminant concentrations conservatively estimate individual external exposures. We tested this assumption by comparing our empirical data to a series of simulations using the ERICA modeling tool. We found that modeled simulations of mean external exposure (10.5 mGy y−1), based on various measures of central tendency, under-predicted mean exposures measured on five of the eight wolves wearing GPS-contaminant monitors (i.e., 12.3, 26.3, 28.0, 28.8 and 35.7 mGy y−1). If under-prediction of exposure occurs for some animals, then arguably the use of averaged contaminant concentrations to predict external exposure is not as conservative as proposed by current risk assessment guidance. Thus, a risk assessor's interpretation of simulated exposures in a screening-level risk analysis might be misguided if contaminant concentrations are based on measures of central tendency. We offer three suggestions for risk assessors to consider in order to reduce the probability of underestimating exposure in a screening-level risk assessment. Keywords: Chernobyl, Wolves, Environmental risk assessment, Model verification, External exposure, Radiation dose

ABSTRACT Sus scrofa is both a destructive invasive species and a popular game animal in many parts of the world, but there is a lack of consistency and accuracy in how scientists and wildlife managers refer to wild‐living members of the species. The growing importance of this invasive species necessitates that scientists, managers, and policy‐makers standardize use of a common name in a taxonomically accurate manner to effectively communicate to the general public and scientific community. In this commentary, we discuss the current terminology used for S. scrofa and, based upon the history of introductions of this species, propose that these animals be referred to as wild pigs within their introduced range unless it is known that the population consists of genetically pure wild boar or domestic pigs that have recently been released and become feral. Use of the term “wild pig” should reduce the potential to misclassify populations as a result of genetic introgression and evolution following release. Furthermore, we recommend that, when appropriate, the terms “nonnative” or “invasive” be included to describe wild pigs in their introduced range to emphasize their negative impacts on natural and anthropogenic environments. The effective control of wild pig populations considered to be invasive will require informed public support and sound scientific management, necessitating clear communication about this species among the research community, wildlife managers, and the general public. © 2016 The Wildlife Society.

Abstract As carrion feeders competing for a limited and ephemeral resource, avian scavengers are ideal model organisms to study mechanisms of niche partitioning. Previous work has focused on observations of species’ interactions at carcasses, and although fruitful, the inclusion of behavior beyond that at carcasses, such as movement patterns, could yield a more comprehensive understanding of mechanisms of foraging niche partitioning. Our goal was to assess how differences in physiological, morphological, and social characteristics between sympatric black vultures (Coragyps atratus) and turkey vultures (Cathartes aura) manifest interspecific variation in movement patterns, foraging habitats, and carcass use to reduce direct competition. Both species are obligate scavengers that co‐occur across much of the New World. Turkey vultures are solitary foragers with enhanced olfactory capabilities and wings optimized for energy‐efficient low‐altitude soaring. Black vultures are aggressive, heavier bodied, and forage socially. We assessed interspecific variation in carcass exploitation using experimental carcass trials encompassing a range of habitats, carcass sizes, and seasons, and used GPS telemetry to compare how soaring conditions influenced daily space use and diurnal patterns of movement activity for each species. Turkey vultures occurred more often at small (i.e., rabbit) carcasses, and those obscured by forest cover than black vultures, and were the first vultures recorded at 94% of carcasses visited by both species. Both species increased movement rates and daily ranges when soaring conditions were favorable; however, turkey vultures had higher travel rates regardless of soaring conditions. Our results suggest the olfactory sense of turkey vultures allows them to locate smaller and visually obscured carcasses more efficiently than black vultures, facilitated by wing morphology that allows them to forage over large areas efficiently. Socially foraging black vultures appear specialized at locating larger carcasses in open habitats, which does not require as much foraging flight as turkey vultures. Our study illustrates how inclusion of movement ecology into niche partitioning studies provides a more complete understanding of the mechanisms of coexistence in avian scavenger guilds. This approach may provide important information to guide conservation efforts, such as supplemental feeding, of imperiled vulture species.

Comprehensive information on crop damage by wildlife species is critical if effective strategies for controlling wildlife damage are to be formulated. Discriminating how landscape composition and configuration attributes influence crop damage is important for implementing landscape management techniques to resolve human–wildlife conflicts. We analyzed crop damage data from 100 corn fields and 60 soybean fields located in the Upper Wabash River Basin of northern Indiana during 2003 and 2004. We used negative binomial regression to model the rate of damage to corn and soybean crops in response to local and landscape variables. Rate of crop damage was best predicted by a combination of local and landscape variables for both corn and soybeans. Models with landscape configuration variables were better able to explain patterns of corn damage, and models with landscape composition variables (specifically, amount of wooded areas) were better able to explain patterns of soybean damage. In general, rate of crop damage was negatively related to size of the crop field and positively related to proportion of a field’s perimeter that was adjacent to wooded areas, amount of wooded areas, amount of forest edge, and mean size of forest patches. Specific associations between local and landscape variables and rates of crop damage may serve as a guide to planting strategies and landscape management to minimize wildlife damage to crops.

Management programs aimed at reducing wildlife damage to row crops rely on information concerning the spatial nature of wildlife damage at local and landscape scales. In this study we explored spatial patterns of wildlife damage within individual corn and soybean fields by describing relationships between specific locations where wildlife damage was recorded and distances from such locations to various habitat types that presumably influenced animal abundance and movements in our study area. Using stratified random sampling, we conducted depredation surveys of 100 corn fields and 60 soybean fields from May through October both in 2003 and 2004 and recorded the specific global positioning satellite (GPS) coordinates of wildlife damage to individual corn and soybean plants. We then generated random point locations in the same fields using a geographic information system (GIS) and evaluated whether damage point locations and random point locations differed with respect to distances to the nearest patches of forest, developed area, or grassland and shrubland habitats. For both crop types, damage point locations were significantly closer to forest patches than were random point locations, but farther from developed areas than random point locations. Logistic regression analyses further indicated that distance to forest influenced the probability of wildlife damage within fields, although pseudo r2 values of the best models were low (0.15). Our results clearly indicated that fi eld portions that were nearest to forested habitats were more likely to suffer wildlife damage than fi eld portions farther from forested habitats. We suggest that targeted removals of depredating species, concentrated along crop-forest interfaces, may be an effective, cost-effective means of reducing corn and soybean damage in areas where wildlife damage is especially problematic.

Wildlife damage to crops is a widespread concern among agricultural producers and wildlife professionals. In the United States, raccoon (Procyon lotor) damage to field corn (Zea maize) has become a serious concern, as raccoon depredation to corn has increased significantly in recent years. However, little information is available to suggest the underlying factors responsible for recent increases in raccoon depredation on agricultural crops because there is a limited understanding of the ecological factors influencing wildlife damage to crops at local scales. During 2004, we initiated a study to elucidate the ecological factors influencing depredation to field corn by raccoons, and, in particular, to determine the relationship between local raccoon abundance and raccoon damage to corn. We used mark-recapture techniques to trap raccoons in 14 forest patches in northern Indiana and estimated raccoon abundance for each patch using the Huggins closed capture maximum likelihood approach in Program MARK™. All cornfields adjacent to the trapped forest patches were surveyed for raccoon damage to obtain patch-specific estimates of crop damage for each patch. We used the best subsets regression moderated by r2 and Akaike's information criterion (AICc), as well as stepwise multiple linear regression to model the influence of raccoon abundance, raccoon sex and age ratios, and landscape characteristics (e.g., forest patch size, isolation) on the amount of damage incurred to cornfields by raccoons. Both best subsets and stepwise regression produced an optimal model that included raccoon abundance and the proportion of forest patch edge bordered by corn as important predictors of raccoon damage to corn. Both raccoon abundance and the proportion of forest patch edge bordered by corn were positively related to the amount of raccoon damage to corn. The results of our study support the supposition that recent increases in raccoon abundance throughout much of the midwestern United States likely have contributed to the concomitant increases in damage to agricultural crops observed in this region. Although current management regimes for raccoons appear to be ineffective at regulating raccoon populations at the landscape level, our results suggest that sustained localized management of raccoon populations may decrease the amount of damage incurred to cornfields at local scales.

Baylisascaris procyonis roundworms, a parasite of raccoons, can infect humans, sometimes fatally. Parasite eggs can remain viable in raccoon latrines for years. To develop a management technique for parasite eggs, we tested anthelmintic baiting. The prevalence of eggs decreased at latrines, and larval infections decreased among intermediate hosts, indicating that baiting is effective.

Abstract Many aspects of parasite biology critically depend on their hosts, and understanding how host‐parasite populations are co‐structured can help improve our understanding of the ecology of parasites, their hosts, and host‐parasite interactions. This study utilized genetic data collected from raccoons (Procyon lotor), and a specialist parasite, the raccoon tick (Ixodes texanus), to test for genetic co‐structuring of host‐parasite populations at both landscape and host scales. At the landscape scale, our analyses revealed a significant correlation between genetic and geographic distance matrices (i.e., isolation by distance) in ticks, but not their hosts. While there are several mechanisms that could lead to a stronger pattern of isolation by distance in tick vs. raccoon datasets, our analyses suggest that at least one reason for the above pattern is the substantial increase in statistical power (due to the ≈8‐fold increase in sample size) afforded by sampling parasites. Host‐scale analyses indicated higher relatedness between ticks sampled from related vs. unrelated raccoons trapped within the same habitat patch, a pattern likely driven by increased contact rates between related hosts. By utilizing fine‐scale genetic data from both parasites and hosts, our analyses help improve our understanding of epidemiology and host ecology.