376 results on '"Jan, Gwénaël"'
Search Results
2. Calcium-rich dairy matrix protects better than mineral calcium against colonic luminal haem-induced alterations in male rats
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Olier, Maïwenn, Naud, Nathalie, Fouché, Edwin, Tondereau, Valérie, Ahn, Ingrid, Leconte, Nadine, Blas-Y-Estrada, Florence, Garric, Gilles, Heliès-Toussaint, Cécile, Harel-Oger, Marielle, Marmonier, Corinne, Théodorou, Vassilia, Guéraud, Françoise, Jan, Gwénaël, and Pierre, Fabrice
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- 2024
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3. Exploring the anti-inflammatory effects of postbiotic proteins from Lactobacillus delbrueckii CIDCA 133 on inflammatory bowel disease model
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Freitas, Andria dos Santos, Barroso, Fernanda Alvarenga Lima, Campos, Gabriela Munis, Américo, Monique Ferrary, Viegas, Rhayane Cristina dos Santos, Gomes, Gabriel Camargos, Vital, Kátia Duarte, Fernandes, Simone Odília Antunes, Carvalho, Rodrigo Dias de Oliveira, Jardin, Julien, Miranda, Ana Paula Gomes dos Santos, Ferreira, Enio, Martins, Flaviano Santos, Laguna, Juliana Guimarães, Jan, Gwénaël, Azevedo, Vasco, and de Jesus, Luís Cláudio Lima
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- 2024
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4. From taste to purchase: Understanding the influence of sensory perceptions and informed tasting on plant-based product purchases - An extension of the theory of planned behavior
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Mustapa, Muhammad Adzran Che, Kallas, Zein, Silande, Charles, Gagnaire, Valérie, Jan, Gwénaël, López-Mas, Laura, and Aguiló-Aguayo, Ingrid
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- 2024
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5. Comprehensive probiogenomics analysis of the commensal Escherichia coli CEC15 as a potential probiotic strain
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da Silva, Tales Fernando, Glória, Rafael de Assis, de Sousa, Thiago Jesus, Americo, Monique Ferrary, Freitas, Andria dos Santos, Viana, Marcus Vinicius Canário, de Jesus, Luís Cláudio Lima, da Silva Prado, Ligia Carolina, Daniel, Nathalie, Ménard, Olivia, Cochet, Marie-Françoise, Dupont, Didier, Jardin, Julien, Borges, Amanda Dias, Fernandes, Simone Odília Antunes, Cardoso, Valbert Nascimento, Brenig, Bertram, Ferreira, Enio, Profeta, Rodrigo, Aburjaile, Flavia Figueira, de Carvalho, Rodrigo Dias Oliveira, Langella, Philippe, Le Loir, Yves, Cherbuy, Claire, Jan, Gwénaël, Azevedo, Vasco, and Guédon, Éric
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- 2023
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6. Functional Swiss-type cheeses promote beneficial effects in mice gut microbiome during homeostasis and inflammation
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Carvalho, Rodrigo Dias de Oliveira, Rabah, Houem, Ariute, Juan Carlos, Aburjaile, Flávia Figueira, Brenig, Bertram, Guédon, Eric, Le Loir, Yves, Jan, Gwénaël, and Azevedo, Vasco
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- 2023
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7. Fat matters: Fermented whole milk potentiates the anti-colitis effect of Propionibacterium freudenreichii
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Mantel, Marine, da Silva, Tales Fernando, Gloria, Rafael, Vassaux, Danièle, Vital, Kátia Duarte, Cardoso, Valbert Nascimento, Fernandes, Simone Odília Antunes, Guédon, Éric, Le Loir, Yves, Faria, Ana Maria Caetano, Rolli-Derkinderen, Malvyne, Azevedo, Vasco, and Jan, Gwénaël
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- 2023
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8. Development of innovative fermented products by exploiting the diversity of immunomodulatory properties and fermentative activity of lactic and propionic acid bacteria
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Illikoud, Nassima, do Carmo, Fillipe Luiz Rosa, Daniel, Nathalie, Jan, Gwénaël, and Gagnaire, Valérie
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- 2023
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9. Propionibacterium freudenreichii: General Characteristics and Probiotic Traits
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de Rezende Rodovalho, Vinícius, primary, Lucas Neres Rodrigues, Diego, additional, Jan, Gwénaël, additional, Le Loir, Yves, additional, Ariston de Carvalho Azevedo, Vasco, additional, and Guédon, Eric, additional
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- 2022
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10. Bacteria, Beneficial: Propionibacterium spp. and Acidipropionibacterium spp.
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Turgay, Meral, primary, Bachmann, Hans-Peter, additional, Irmler, Stefan, additional, von Ah, Ueli, additional, Fröhlich-Wyder, Marie-Therese, additional, Falentin, Hélène, additional, Deutsch, Stéphanie-Marie, additional, Jan, Gwénaël, additional, and Thierry, Anne, additional
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- 2022
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11. Improving the drying of Propionibacterium freudenreichii starter cultures
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Jeantet, Romain and Jan, Gwénaël
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- 2021
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12. Postbiotic effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on amurine model of 5-FU-induced intestinal mucositis
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Glória, Rafael de Assis, primary, Silva, Tales Fernando da, additional, Gomes, Tomás Andrade Magalhães, additional, Vital, Kátia Duarte, additional, Fernandes, Simone Odília Antunes, additional, Cardoso, Valbert Nascimento, additional, Ferreira, Ênio, additional, Chatel, Jean-Marc, additional, Langella, Philippe, additional, Cherbuy, Claire, additional, Loir, Yves Le, additional, Jan, Gwénaël, additional, Guédon, Éric, additional, and Azevedo, Vasco Ariston de Carvalho, additional
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- 2023
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13. Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
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Gaucher, Floriane, Rabah, Houem, Kponouglo, Koffigan, Bonnassie, Sylvie, Pottier, Sandrine, Dolivet, Anne, Marchand, Pierre, Jeantet, Romain, Blanc, Philippe, and Jan, Gwénaël
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- 2020
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14. Benefits and drawbacks of osmotic adjustment in Propionibacterium freudenreichii
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Gaucher, Floriane, Bonnassie, Sylvie, Rabah, Houem, Leverrier, Pauline, Pottier, Sandrine, Jardin, Julien, Briard-Bion, Valérie, Marchand, Pierre, Jeantet, Romain, Blanc, Philippe, and Jan, Gwénaël
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- 2019
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15. Propionic fermentation by the probiotic Propionibacterium freudenreichii to functionalize whey
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Huang, Song, Rabah, Houem, Ferret-Bernard, Stéphanie, Le Normand, Laurence, Gaucher, Floriane, Guerin, Sylvie, Nogret, Isabelle, Le Loir, Yves, Chen, Xiao Dong, Jan, Gwénaël, Boudry, Gaëlle, and Jeantet, Romain
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- 2019
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16. Innovations and prospects
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Fenelon, Mark A., primary, Murphy, Eoin G., additional, Martins, Evandro, additional, Fialho, Tatiana Lopes, additional, Schuck, Pierre, additional, de Carvalho, Antônio Fernandes, additional, Stephani, Rodrigo, additional, Perrone, Ítalo Tuler, additional, Ho, Thao Minh, additional, Zou, Zhengzheng, additional, Bhandari, Bhesh, additional, Bansal, Nidhi, additional, Tanguy, Gaëlle, additional, Méjean, Serge, additional, Jeantet, Romain, additional, Dolivet, Anne, additional, Huang, Song, additional, Jan, Gwénaël, additional, and Fenelon, Mark A., additional
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- 2020
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17. Propionibacterium freudenreichii CIRM-BIA 129 mitigates colitis through S layer protein B-dependent epithelial strengthening.
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Mantel, Marine, Durand, Tony, Bessard, Anne, Pernet, Ségolène, Beaudeau, Julie, Guimaraes-Laguna, Juliana, Maillard, Marie-Bernadette, Guédon, Eric, Neunlist, Michel, Loir, Yves Le, Jan, Gwénaël, and Rolli-Derkinderen, Malvyne
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COLITIS ,INTESTINAL barrier function ,PROPIONIBACTERIUM ,GENE expression ,SODIUM sulfate ,OCCLUDINS - Abstract
The growing incidence of human diseases involving inflammation and increased gut permeability makes the quest for protective functional foods more crucial than ever. Propionibacterium freudenreichii (P. freudenreichii) is a beneficial bacterium used in the dairy and probiotic industries. Selected strains exert anti-inflammatory effects, and the present work addresses whether the P. freudenreichii CIRM-BIA129, consumed daily in a preventive way, could protect mice from acute colitis induced by dextran sodium sulfate (DSS), and more precisely, whether it could protect from intestinal epithelial breakdown induced by inflammation. P. freudenreichii CIRM-BIA129 mitigated colitis severity and inhibited DSS-induced permeability. It limited crypt length reduction and promoted the expression of zonula occludens-1 (ZO-1), without reducing interleukin-1b mRNA (il-1b ) expression. In vitro, P. freudenreichii CIRM-BIA129 prevented the disruption of a Caco-2 monolayer induced by proinflammatory cytokines. It increased transepithelial electrical resistance (TEER) and inhibited permeability induced by inflammation, along with an increased ZO-1 expression. Extracellular vesicles (EVs) from P. freudenreichii CIRM-BIA129, carrying the surface layer protein (SlpB), reproduced the protective effect of P. freudenreichii CIRM-BIA129. A mutant strain deleted for slpB (DslpB), or EVs from this mutant strain, had lost their protective effects and worsened both DSS-induced colitis and inflammation in vivo. These results shown that P. freudenreichii CIRM-BIA129 daily consumption has the potential to greatly alleviate colitis symptoms and, particularly, to counter intestinal epithelial permeability induced by inflammation by restoring ZO-1 expression through mechanisms involving S-layer protein B. They open new avenues for the use of probiotic dairy propionibacteria and/or postbiotic fractions thereof, in the context of gut permeability. NEW & NOTEWORTHY Propionibacterium freudenreichii reduces dextran sodium sulfate (DSS)-induced intestinal permeability in vivo. P. freudenreichii does not inhibit inflammation but damages linked to inflammation. P. freudenreichii inhibits intestinal epithelial breakdown through S-layer protein B. The protective effects of P. freudenreichii depend on S-layer protein B. Extracellular vesicles from P. freudenreichii CB 129 mimic the protective effect of the probiotic. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Comprehensive Probiogenomics Analysis of the Commensal Escherichia Coli CEC15 as a Potential Probiotic Strain
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Silva, Tales Fernando da, primary, Glória, Rafael de Assis, additional, Sousa, Thiago de Jesus, additional, Americo, Monique Ferrary, additional, Freitas, Andria dos Santos, additional, Viana, Marcus Vinicius Canário, additional, de Jesus, Luis Claudio Lima, additional, Prado, Ligia Carolina da Silva, additional, Daniel, Nathalie, additional, Ménard, Olivia, additional, Cochet, Marie-Françoise, additional, Dupont, Didier, additional, Jardin, Julien, additional, Borges, Amanda Dias, additional, Fernandes, Simone Odília Antunes, additional, Cardoso, Valbert Nascimento, additional, Brenig, Bertram, additional, Ferreira, Enio, additional, Profeta, Rodrigo, additional, Aburjaile, Flavia Figueira, additional, Carvalho, Rodrigo Dias de Oliveira, additional, Langella, Philippe, additional, Loir, Yves Le, additional, Cherbuy, Claire, additional, Jan, Gwénaël, additional, Azevedo, Vasco, additional, and Guédon, Éric, additional
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- 2023
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19. Development of innate immune memory by non-immune cells during Staphylococcus aureus infection depends on reactive oxygen species
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Chaumond, Emmanuel, primary, Peron, Sandrine, additional, Daniel, Nathalie, additional, Le Gouar, Yann, additional, Guédon, Éric, additional, Williams, David L., additional, Le Loir, Yves, additional, Jan, Gwénaël, additional, and Berkova, Nadia, additional
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- 2023
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20. Double use of concentrated sweet whey for growth and spray drying of probiotics: Towards maximal viability in pilot scale spray dryer
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Huang, Song, Méjean, Serge, Rabah, Houem, Dolivet, Anne, Le Loir, Yves, Chen, Xiao Dong, Jan, Gwénaël, Jeantet, Romain, and Schuck, Pierre
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- 2017
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21. Beneficial propionibacteria: from traditional cheeses to next generation probiotics
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Jan, Gwénaël, Foligné, Benoît, Gagnaire, Valérie, Boudry, Gaëlle, Azevedo, Vasco, Guédon, Eric, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte
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[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,propionibacteria ,traditional cheese ,inflammatory response ,immunomodulation ,Colitis ,fermented vegetable ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,probiotic - Abstract
International audience; Swiss cheeses have been produced for centuries in a traditional way and their typical opening indicates the presence of propionibacteria. These last are indeed present in raw milk, their growth is favored by the specific technology of Swiss-type cheeses, and their high population in the ripened cheese is responsible for its characteristic sensory properties, including the opening and the aroma. In addition to this long history of safe use and consumption, they constitute a source of nutraceuticals. The specific propionic fermentation leads to the production of short chain fatty acids as main final obligatory products. These exert beneficial effects including nourishment of digestive epithelial cells, as well as promotion of differentiation and of apoptotic depletion of colon cancer cells. Propionibacteria also produce vitamins of the B group, which enhances the nutritional value of the food products they ferment. Some selected strains were moreover shown to produce immunomodulins, proteins able to modify the secretion of cytokines in human immune cells. These last were identified as proteinaceous components of propionibacteria surface layer. They also produce extracellular vesicles which modulate in vitro inflammation and the NFκB pathway in human intestinal epithelial cells. Accordingly, such strains modulate the immune response and mitigate inflammation. In mice, they prevent both mucositis and colitis, via such immunomodulins limiting acute inflammatory response and protecting the gut epithelial barrier. In pigs, their consumption also modulates the inflammatory response and the lymphocyte populations. Finally, clinical studies dealing with consumption of propionibacteria whey cultures suggest a beneficial role in the context of IBD. Propionibacteria strains were recently identified in the gut microbiota of healthy newborns, yet not in that of children with necrotizing enterocolitis. Indeed, propionibacteria were detected in human fecal samples, which means that propionibacteria may be traditional cheese starters or next generation probiotics. The growing knowledge of these peculiar actinobacteria, including their great strain-diversity, opens new avenues for the development of functional foods and of food supplements, in a general context of increasing incidence of ailments that include an inflammatory component. Moreover, their ability to ferment both dairy and vegetable matrices is presently investigated and the first promising advances open new perspectives for the development of functional fermented vegetable products.
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- 2023
22. Functional Swiss-type experimental cheeses diet promotes beneficial effects in mice gut microbiome during homeostasis and inflammation
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Carvalho, Rodrigo, primary, Rabah, Houem, additional, Carmo, Fillipe Luiz Rosa, additional, Ariute, Juan Carlos, additional, Aburjaile, Flávia Figueira, additional, Brenig, Bertram, additional, Guédon, Eric, additional, Loir, Yves Le, additional, Jan, Gwénaël, additional, and Azevedo, Vasco, additional
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- 2023
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23. Double use of highly concentrated sweet whey to improve the biomass production and viability of spray-dried probiotic bacteria
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Huang, Song, Cauty, Chantal, Dolivet, Anne, Le Loir, Yves, Chen, Xiao Dong, Schuck, Pierre, Jan, Gwénaël, and Jeantet, Romain
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- 2016
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24. Metagenomic Approaches for Investigating the Role of the Microbiome in Gut Health and Inflammatory Diseases
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Carvalho, Rodrigo, primary, Carmo, Fillipe, additional, Heloisa, Sara, additional, Cordeiro, Barbara, additional, Vaz, Aline, additional, Gimenez, Enrico, additional, Goulart, Luis, additional, Góes-Neto, Aristóteles, additional, Loir, Yves Le, additional, Jan, Gwénaël, additional, and Azevedo, Vasco, additional
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- 2018
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25. Different culture media and purification methods unveil the core proteome of Propionibacterium freudenreichii-derived extracellular vesicles
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Rodovalho, Vinícius de Rezende, primary, da Luz, Brenda Silva Rosa, additional, Nicolas, Aurélie, additional, Jardin, Julien, additional, Briard-Bion, Valérie, additional, Folador, Edson Luiz, additional, Santos, Anderson Rodrigues, additional, Jan, Gwénaël, additional, Loir, Yves Le, additional, Azevedo, Vasco Ariston de Carvalho, additional, and Guédon, Éric, additional
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- 2023
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26. Single-strain starter experimental cheese reveals anti-inflammatory effect of Propionibacterium freudenreichii CIRM BIA 129 in TNBS-colitis model
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Plé, Coline, Richoux, Romain, Jardin, Julien, Nurdin, Marine, Briard-Bion, Valérie, Parayre, Sandrine, Ferreira, Stéphanie, Pot, Bruno, Bouguen, Guillaume, Deutsch, Stéphanie-Marie, Falentin, Hélène, Foligné, Benoit, and Jan, Gwénaël
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- 2015
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27. Surface proteins of Propionibacterium freudenreichii are involved in its anti-inflammatory properties
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Le Maréchal, Caroline, Peton, Vincent, Plé, Coline, Vroland, Christophe, Jardin, Julien, Briard-Bion, Valérie, Durant, Gaël, Chuat, Victoria, Loux, Valentin, Foligné, Benoit, Deutsch, Stéphanie-Marie, Falentin, Hélène, and Jan, Gwénaël
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- 2015
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28. Dairy starters and fermented dairy products modulate gut mucosal immunity
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Illikoud, Nassima, primary, Mantel, Marine, additional, Rolli-Derkinderen, Malvyne, additional, Gagnaire, Valérie, additional, and Jan, Gwénaël, additional
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- 2022
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29. Inflammatory bowel disease therapeutic strategies by modulation of the microbiota: how and when to introduce pre-, pro-, syn-, or postbiotics?
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Lê, Amélie, primary, Mantel, Marine, additional, Marchix, Justine, additional, Bodinier, Marie, additional, Jan, Gwénaël, additional, and Rolli-Derkinderen, Malvyne, additional
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- 2022
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30. Data from a proteomic comparative analysis highlight differential adaptation of Lactobacillus delbrueckii subsp. bulgaricus to cow milk versus to soy milk environments
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Jan, Gwénaël, primary, Tarnaud, Florian, additional, do Carmo, Fillipe Luiz Rosa, additional, Illikoud, Nassima, additional, Canon, Fanny, additional, Jardin, Julien, additional, Briard-Bion, Valérie, additional, Guyomarc'h, Fanny, additional, and Gagnaire, Valérie, additional
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- 2022
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31. The dairy bacterium Propionibacterium freudenreichii against colitis and mucositis: a key role of the surface layer protein SlpB
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Jan, Gwénaël, Foligné, Benoît, Rosa Do Carmo, Fillipe Luiz, Rabah, Houem, Gaucher, Floriane, Azevedo, Vasco, Guédon, Eric, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil] (UFMG), and Giboulot, Anne
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Mucositis ,[SDV] Life Sciences [q-bio] ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Microbiota ,[SDV]Life Sciences [q-bio] ,Emmental ,Colitis ,Immun system ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,Gut inflammation ,Propionibacteria freudenreichii - Abstract
International audience; ContextGut inflammation constitutes a growing health concern in developed countries. It may consist in spontaneous ailments of the gut, involving both the host immune system and microbiota, such as IBD, including ulcerative colitis and Crohn’s disease. It may be caused by a medical treatment, such as mucositis induced by cancer chemotherapy and/or radiotherapy. It coincides with a dysbiosis including a lack of anti-inflammatory bacteria. As an example, propionibacteria are lacking in the microbiota of newborns that develop necrotizing enterocolitis.Methods and resultsWe thus focused on the immunomodulatory properties of GRAS propionibacteria. Selected strains of Propionibacterium freudenreichii induced the regulatory IL-10 cytokine in human immune cells (Foligné et al., 2010, 2013), depending on surface proteins (Le Marechal et al., 2015). Mutation of the slpB gene suppressed this immunomodulatory effect and the resulting slpB mutant induced a rather proinflammatory response (Deutsch et al., 2017). Consumption of wild-type P. freudenreichii protected from colitis induced by both TNBS and by DSS. It alleviated severity of symptoms, modulated local and systemic inflammation, as well as colonic oxidative stress and epithelial cell damages (Plé et al., 2015, 2016; Rabah et al., 2020). Accordingly, consumption of Lactococcus lactis NCDO 2118 harboring pXIES-SEC:slpB and expressing the propionibacterial SlpB reduced severity of colitis, lowered weight loss, disease activity index, shortening of the colon length, and histopathological score, compared with mice treated with L. lactis NCDO 2118 wild-type strain.In the context of mucositis induced by the chemotherapy 5-FU, P. freudenreichii prevented weight loss, reduced inflammation and consequently intestinal damages. It regulated key markers, including Claudin-1 and IL-17a genes, as well as IL-12 and IL-1β cytokines levels (Cordeiro et al., 2018). Mutant strain slpB displayed opposite regulatory effect on cld1 expression and on IL-12 levels, and failed to afford protection towards 5-FU-mucositis (do Carmo et al., 2019).ConclusionThis work emphasizes the importance of SlpB in P. freudenreichii ability to reduce both mucositis and colitis inflammation. It opens perspectives for the development of probiotic products aimed at decreasing side effects of chemotherapy and at helping treatment of colitis, thanks to GRAS bacteria.ReferencesCordeiro, B. F., Oliveira, E. R., Silva, D., H, S., Savassi, B. M., Acurcio, L. B., et al. (2018). Whey Protein Isolate-Supplemented Beverage, Fermented by Lactobacillus casei BL23 and Propionibacterium freudenreichii 138, in the Prevention of Mucositis in Mice. Front. Microbiol. 9. doi:10.3389/fmicb.2018.02035.Deutsch, S.-M., Mariadassou, M., Nicolas, P., Parayre, S., Le Guellec, R., Chuat, V., et al. (2017). Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study. Sci Rep 7, 46409. doi:10.1038/srep46409.do Carmo, F. L. R., Rabah, H., Cordeiro, B. F., da Silva, S. H., Pessoa, R. M., Fernandes, S. O. A., et al. (2019). Probiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy. Oncotarget 10, 7198–7219. doi:10.18632/oncotarget.27319.Foligné, B., Breton, J., Mater, D., and Jan, G. (2013). Tracking the microbiome functionality: focus on Propionibacterium species. Gut 62, 1227–1228.Foligné, B., Deutsch, S. M., Breton, J., Cousin, F. J., Dewulf, J., Samson, M., et al. (2010). Promising immunomodulatory effects of selected strains of dairy propionibacteria as evidenced in vitro and in vivo. Appl.Environ.Microbiol. 76, 8259–8264.Le Marechal, C., Peton, V., Ple, C., Vroland, C., Jardin, J., Briard-Bion, V., et al. (2015). Surface proteins of Propionibacterium freudenreichii are involved in its anti-inflammatory properties. J.Proteomics. 113C, 447–461.Plé, C., Breton, J., Richoux, R., Nurdin, M., Deutsch, S.-M., Falentin, H., et al. (2016). Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: Reverse engineering development of an anti-inflammatory cheese. Mol Nutr Food Res 60, 935–948. doi:10.1002/mnfr.201500580.Plé, C., Richoux, R., Jardin, J., Nurdin, M., Briard-Bion, V., Parayre, S., et al. (2015). Single-strain starter experimental cheese reveals anti-inflammatory effect of Propionibacterium freudenreichii CIRM BIA 129 in TNBS-colitis model. Journal of Functional Foods 18, 575–585. doi:10.1016/j.jff.2015.08.015.Rabah, H., do Carmo, F. L. R., Carvalho, R. D. de O., Cordeiro, B. F., da Silva, S. H., Oliveira, E. R., et al. (2020). Beneficial Propionibacteria within a Probiotic Emmental Cheese: Impact on Dextran Sodium Sulphate-Induced Colitis in Mice. Microorganisms 8, 380. doi:10.3390/microorganisms8030380.- PF induces IL-10 in human PBMCs- Surface layer extraction reduces IL-10 induction- Extracted surface layer induces IL-10- Mutation of the SlpB gene reduces IL-10 induction- PF prevents colitis- PF prevents mucositis- PF slpB mutant does not prevent mucositis- Lactococcus lactis expressing SlpB prevents colitis
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- 2022
32. The stressing life of Lactobacillus delbrueckii subsp. bulgaricus in soy milk
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Jan, Gwénaël, primary, Tarnaud, Florian, additional, Rosa do Carmo, Fillipe Luiz, additional, Illikoud, Nassima, additional, Canon, Fanny, additional, Jardin, Julien, additional, Briard-Bion, Valérie, additional, Guyomarc'h, Fanny, additional, and Gagnaire, Valérie, additional
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- 2022
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33. A Unique Enhancement of Propionibacterium freudenreichii’s Ability to Remove Pb(II) from Aqueous Solution by Tween 80 Treatment
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George, Fanny, primary, Titécat, Marie, additional, Barois, Nicolas, additional, Daniel, Catherine, additional, Garat, Anne, additional, Jan, Gwénaël, additional, and Foligné, Benoît, additional
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- 2022
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34. List of Contributors
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Altenbach, Susan B., primary, Arena, Simona, additional, Barros-Velázquez, Jorge, additional, Bassols, Anna, additional, Bendixen, Emoke, additional, Böhme, Karola, additional, Brunisholz, René, additional, Calo-Mata, Pilar, additional, Campos, Alexandre, additional, Cañas, Benito, additional, Carrera, Mónica, additional, Claudius, Marondedze, additional, Costa-Lima, Bruno R.C., additional, Cristobal, Susana, additional, D’Amato, Alfonsina, additional, D’Ambrosio, Chiara, additional, de Almeida, André M., additional, Di Luccia, Aldo, additional, Drissner, David, additional, Fernández-No, Inmaculada C., additional, Ferranti, Pasquale, additional, Gagaoua, Mohammed, additional, Gagliardi, Rosa, additional, Gagnaire, Valérie, additional, Gallardo, José M., additional, Hashiguchi, Akiko, additional, Haynes, Paul A., additional, Jan, Gwénaël, additional, Josić, Djuro, additional, Komatsu, Setsuko, additional, Kraljevic Pavelić, Sandra, additional, Kuruvilla, Jacob, additional, la Gatta, Barbara, additional, Ludivine, Thomas, additional, Mamone, Gianfranco, additional, Mann, Karlheinz, additional, Martinović, Tamara, additional, Méndez, Lucía, additional, Miller, Ingrid, additional, Mirzaei, Mehdi, additional, Montowska, Magdalena, additional, Nair, Mahesh N., additional, Nitride, Chiara, additional, Ortea, Ignacio, additional, Pazos, Manuel, additional, Pechanova, Olga, additional, Pechan, Tibor, additional, Pedreschi, Romina, additional, Peršurić, Željka, additional, Picard, Brigitte, additional, Picariello, Gianluca, additional, Raftos, David, additional, Renzone, Giovanni, additional, Rešetar, Dina, additional, Righetti, Pier Giorgio, additional, Rodrigues, Pedro M., additional, Rodríguez, Eva M., additional, Rusco, Giusy, additional, Rutigliano, Mariacinzia, additional, Salzano, Anna Maria, additional, Sassi, Mauro, additional, Satoh, Rie, additional, Scaloni, Andrea, additional, Schrama, Denise, additional, Suman, Surendranath P., additional, Teshima, Reiko, additional, Thompson, Emma, additional, Tresse, Odile, additional, Vaibhav, Vineet, additional, Wes Schilling, M., additional, Willows, Robert D., additional, Worden, Paul, additional, and Wu, Yunqi, additional
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- 2017
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35. Proteomics of Fermented Milk Products
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Gagnaire, Valérie, primary and Jan, Gwénaël, additional
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- 2017
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36. Staphylococcus aureus proteins differentially recognized by the ovine immune response in mastitis or nasal carriage
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Seyffert, Nubia, Le Maréchal, Caroline, Jardin, Julien, McCulloch, John A., Rosado, Fabio R., Miyoshi, Anderson, Even, Sergine, Jan, Gwenaël, Berkova, Nadia, Vautor, Eric, Thiéry, Richard, Azevedo, Vasco, and Le Loir, Yves
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- 2012
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37. La fermentation comme moyen de rendre un aliment fonctionnel
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Jan, Gwénaël, Gagnaire, Valérie, and Giboulot, Anne
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[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,fonctionnalité alimentaire ,bactérie lactique ,aliment fermenté ,[SDV.IDA] Life Sciences [q-bio]/Food engineering ,probiotique ,fermentation ,produit laitier - Published
- 2022
38. Combined protective effects of dairy propionic bacteria and n-6 polyunsaturated fatty acids on the intestinal epithelial barrier
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Marine, Mantel, Mahe, Maxime, Illikoud, Nassima, Cenac, Nicolas, Neunlist, Michel, Jan, Gwénaël, Rolli-Derkinderen, Malvyne, and Giboulot, Anne
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[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,n-6 polyunsaturated fatty acids ,intestinal epithelial barrier ,Propionibacterium feudenreichii ,Inflammatory bowel disease ,dairy propionic bacteria - Abstract
Introduction Intestinal epithelial barrier (IEB) integrity plays a central role in the pathogenesis of inflammatory bowel disease (IBD). There is a growing interest in dietary compounds that can strengthen IEB. First, candidate strains of dairy propionic bacteria such as Propionibacterium freudenreichii are able to reduce inflammation and improve barrier strength. Knowing that certain products of omega-6 polyunsaturated fatty acid (n-6 PUFA) metabolism also reinforce IEB, and that bacteria can modulate the bioavailability of host lipid compounds, this project aims to identify strains of P. freudenreichii that could protect against colitis by strengthening IEB and increasing the bioavailability of certain n-6 PUFA. MethodsA screening of thirty-three strains of P. freudenreichii was performed based on the following criteria: 1) characterization of the ability to induce production of anti-inflammatory cytokines (IL-10) by stimulation of human PBMCs 2) identification of surface proteins (S-layer protein) responsible for anti-inflammatory properties 3) measurement of their ability to increase the bioavailability of certain n-6 PUFAs, such as conjugated linoleic acids (CLAs). The ability of the selected fermented products to prevent the inflammation-induced increase in permeability of an IEB was then studied. ResultsAll strains are able to induce IL-10 production and release CLAs. Ten strains show an anti-inflammatory S-layer profile. Some fermented products significantly prevent the inflammation-induced increase in permeability, and may involve surface proteins and bioactive lipids. DiscussionThese results will allow the selection of strains with anti-inflammatory as well as pro-repair potential to be tested in vivo to reduce colitis, with or without n-6 PUFA supplementation.
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- 2022
39. Environmental conditions modulate the protein content and immunomodulatory activity of extracellular vesicles produced by the probiotic Propionibacterium freudenreichii
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Rodovalho, Vinícius de Rezende, Luz, Brenda Silva Rosa Da, Nicolas, Aurélie, Rosa Do Carmo, Fillipe Luiz, Jardin, Julien, Briard-Bion, Valérie, Jan, Gwénaël, Le Loir, Yves, Ariston de Carvalho Azevedo, Vasco, Guédon, Eric, and Giboulot, Anne
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comparative proteomics ,protein-protein interaction ,Immunomodulation ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,growth conditions ,inflammatory response ,extracellular vesicle ,extracellular vesicles ,membrane vesicle ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,Propionibacterium freudenreichii ,probiotic ,anti-inflammatory - Abstract
Propionibacterium freudenreichii is a probiotic Gram-positive bacterium with promising immunomodulatory properties. It modulates regulatory cytokines, mitigates the inflammatory response in vitro and in vivo These properties were initially attributed to specific bacterial surface proteins. Recently, we showed that extracellular vesicles (EVs) produced by P. freudenreichii CIRM-BIA129 mimic the immunomodulatory features of parent cells in vitro (i.e. modulating NF-κB transcription factor activity and IL-8 release) which underlies the role of EVs as mediators of the probiotic effects of the bacterium. The modulation of EV properties, and particularly of those with potential therapeutic applications such as the EVs produced by the probiotic P. freudenreichii, is one of the challenges in the field to achieve efficient yields with the desired optimal functionality. Here we evaluated whether the culture medium in which the bacteria are grown could be used as a lever to modulate the protein content and hence the properties of P. freudenreichii CIRM-BIA129 EVs. The physical, biochemical and functional properties of EVs produced from cells cultivated on laboratory Yeast Extract Lactate (YEL) medium and cow milk ultrafiltrate (UF) medium were compared. UF-derived EVs were more abundant, smaller in diameter and displayed more intense anti-inflammatory activity than YEL-derived EVs. Furthermore, the growth media modulated EV content in terms of both the identities and abundances of their protein cargos, suggesting different patterns of interaction with the host. Proteins involved in amino acid metabolism and central carbon metabolism were modulated, as were the key surface proteins mediating host-propionibacteria interactions.Importance Extracellular vesicles (EVs) are cellular membrane-derived nanosized particles that are produced by most cells in all three kingdoms of life. They play a pivotal role in cell-cell communication through their ability to transport bioactive molecules from donor to recipient cells. Bacterial EVs are important factors in host-microbe interactions. Recently we have shown that EVs produced by the probiotic P. freudenreichii exhibited immunomodulatory properties. We evaluate here the impact of environmental conditions, notably culture media, on P. freudenreichii EV production and function. We show that EVs display considerable differences in protein cargo and immunomodulation depending on the culture medium used. This work offers new perspectives for the development of probiotic EV-based molecular delivery systems, and reinforces the optimization of growth conditions as a tool to modulate the potential therapeutic applications of EVs.
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- 2022
40. Immunomodulatory role of Propionibacterium freudenreichii extracellular vesicles
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Silva, Tales, de Rezende Rodovalho, Vinícius, Luz, Brenda Silva Rosa Da, Rosa Do Carmo, Fillipe Luiz, Nicolas, Aurélie, Jardin, Julien, Briard -Bion, Valérie, Jan, Gwénaël, Le Loir, Yves, Azevedo, Vasco, Guédon, Eric, and Giboulot, Anne
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immunomodulation ,extracellular vesicles ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,Propionibacterium freudenreichii - Abstract
The role of Propionibacterium freudenreichii in mitigating inflammation has been a subject of study for many years. Lately, it has been shown that immunomodulation properties are strain- specific and that the major responsible for inflammatory modulation ofimmunomodulation by strain CIRM-BIA129 is the presence of the surface layer protein B (SlpB), which has presented immunomodulation even when expressed in other bacteria. Extracellular vesicles (EVs) are nanosized spherical structures, produced by organisms of all kingdoms, including bacteria. They have been associated with inter-organism communication, pathogenesis, competition, and immunomodulation. Recent studies were aiming to address the role of EVs, in the probiotic effects of bacteria. The properties of P. freudenreichii CIRM-BIA129- derived EVs have been investigated. EVs produced by CIRM-BIA129 cultured in milk ultrafiltrate medium (UF) have been characterized by regarding size and morphology. UF-derived EVs displayed a monodisperse pattern with a modal size of 84.80 ± 2.34 nm. They are composed of a wide variety of proteins, mainly involved in metabolic processes, cellular processes and signaling, and storage and processing of information. A, among these proteins, SlpB was found in high abundance. P. freudenreichii EVs were able to inhibit, in a dose dependent manner, the increase of IL-8 production in HT-29 cells induced with LPS, due to NF-KB pathway inhibition, without causing cell cytotoxicity. EVs produced by a CIRM-BIA129 mutant strain with a knockout for SlpB showed a less efficient reduction in IL-8 production. Results have shown that the environmental conditions are able to modify EVs content and, consequently, their immunomodulatory effects. A change in the growth medium, from UF to YEL (yeast extract-lactate) showed a lower production of EVs, with a slightly larger size. EVs produced in YEL did not perform as well in the inhibition of the NF-KB pathway and had no effect on IL-8 production. Recent results have shown that P. freudenreichii EVs were able to protect Caco-2 cells from inflammation-induced excessive increasing permeability. These Altogether, these results show that Evs produced by beneficial propionibacteria are able to generate immunomodulationtrigger immunomodulation, similar to the parental strain. As for the SlpB role in EVs immunomodulation
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- 2022
41. Developing innovative plant-based added-value food products through the promotion of LOCAL Mediterranean NUT and LEGUME crops
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Gagnaire, Valérie, Jan, Gwénaël, and Giboulot, Anne
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Nut ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,Innovation & Sustainability ,Local Agri-food System ,Fermented food ,Legume ,Mediterraean - Abstract
The aim of LOCALNUTLEG project is to empower local Mediterranean nuts and legumes with quality characteristics that depend exclusively on the territory on which they are produced through the development of nniovative plant-based food products, fermented or not. To do so, LOCALNUTLEG will promote local Mediterranean nuts and legumes with a legal registered trademark (Protected Designation of Origin (PDO) or Protected Geographical Indication (PGI)) or autochthonous identity and will represent a potential opportunity to Mediterranean countries to balance the sustainability of Agri-food resources and dietary habits considering the economical and health impact of the new developed products. The project aims to develop innovative food products by the identification of local nut and legumes varieties tied to Mediterranean gastronomic culture, providing the maximum amount of nutrients and bioactive compounds, and to promote Mediterranean diet through plant-based food products., Le but de LOCALNUTLEG est d’autonomiser des filières locales méditerranéennes de produits végétaux, fermentés ou non, sous signe de qualité géographique ou avec une identité locale forte. Pour cela le projet développera des produits alimentaires innovants à partir des variétés locales de fruits secs et légumes secs liés à la culture gastronomique méditerranéennes, riches en nutriments et composés bioactifs. Ces produits seront une opportunité de conjuguer la durabilité des matières premières agricoles locales et des habitudes alimentaires pour des produits sains et contribuant à l’économie locale.
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- 2022
42. The dairy probiotic bacterium Propionibacterium freudenreichii against colitis and mucositis: a key role of the surface layer protein SlpB
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Jan, Gwénaël, Foligné, Benoît, Rosa Do Carmo, Fillipe Luiz, Rodovalho, Vinícius, Rabah, Houem, Gaucher, Floriane, Azevedo, Vasco, Guédon, Eric, Giboulot, Anne, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), and Universidade Federal de Minas Gerais [Belo Horizonte] (UFMG)
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Inflammation ,Mucositis ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Vesicle ,Gut ,Colitis ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology - Abstract
International audience; IntroductionGut inflammation constitutes a growing health concern in developed countries. It may consist in spontaneous ailments of the gut, involving both the host immune system and microbiota, such as IBD, including ulcerative colitis and Crohn’s disease. It may be caused by a medical treatment, such as mucositis induced by cancer chemotherapy and/or radiotherapy. It coincides with a dysbiosis including a lack of anti-inflammatory bacteria. As an example, propionibacteria are lacking in the microbiota of newborns that develop necrotizing enterocolitis.Methods Dairy propionibacteria strains were tested in vitro on human PBMCs with respect to their ability to induce immunomodulatory cytokines. Selected strains were then tested in vitro with respect to their ability to protect mice from TNBS-induced colitis, from DSS-induced colitis, and from 5FU-induced mucositis.ResultsWe thus focused on the immunomodulatory properties of GRAS propionibacteria. Selected strains of Propionibacterium freudenreichii induced the regulatory IL-10 cytokine in human immune cells (Foligné et al., 2010, 2013), depending on surface proteins (Le Marechal et al., 2015). Mutation of the slpB gene suppressed this immunomodulatory effect and the resulting slpB mutant induced a rather proinflammatory response (Deutsch et al., 2017). Consumption of wild-type P. freudenreichii protected from colitis induced by both TNBS and by DSS. It alleviated severity of symptoms, modulated local and systemic inflammation, as well as colonic oxidative stress and epithelial cell damages (Plé et al., 2015, 2016; Rabah et al., 2020). Accordingly, consumption of Lactococcus lactis NCDO 2118 harboring pXIES-SEC:slpB and expressing the propionibacterial SlpB reduced severity of colitis, lowered weight loss, disease activity index, shortening of the colon length, and histopathological score, compared with mice treated with L. lactis NCDO 2118 wild-type strain (Belo et al., 2021).In the context of mucositis induced by the chemotherapy 5-FU, P. freudenreichii prevented weight loss, reduced inflammation and consequently intestinal damages. It regulated key markers, including Claudin-1 and IL-17a genes, as well as IL-12 and IL-1β cytokines levels (Cordeiro et al., 2018). Mutant strain slpB displayed opposite regulatory effect on cld1 expression and on IL-12 levels, and failed to afford protection towards 5-FU-mucositis (do Carmo et al., 2019).P. freudenreichii was further shown to produce extracellular vesicles (EVs), which mimic the immunomodulatory features of propionibacteria in vitro by modulating NF-κB transcription factor activity and IL-8 release in cultured human intestinal epithelial cells (Rodovalho et al., 2020). Proteomic analysis revealed presence of SlpB in these EVs.DiscussionThis work emphasizes the importance of SlpB in P. freudenreichii ability to reduce both mucositis and colitis inflammation. It opens perspectives for the development of probiotic products aimed at decreasing side effects of chemotherapy and at helping treatment of colitis, thanks to GRAS bacteria.
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- 2022
43. Do probiotic dairy starters adapt to vegetable milks?
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Illikoud, Nassima, Tarnaud, Florian, Gaucher, Floriane, Rosa Do Carmo, Fillipe Luiz, Jardin, Julien, Briard-Bion, Valérie, Guyomarc'H, Fanny, Gagnaire, Valérie, Jan, Gwénaël, and Giboulot, Anne
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Lactobacillus ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,proteome ,Propionibacterium ,soy milk ,probiotic - Abstract
In a general context of food transition, plant-based fermented products experience a considerable development. This results from evolution of consumers’ habits, demanding healthy products with a low environmental impact. This requires research and development, and thus higher scientific knowledge. Fermented soy milks constitute a favorable alternative source of live probiotics. Lactobacillus delbrueckii subsp. bulgaricus and Propionibacterium freudenreichii are extensively used in probiotic dairy products. Their adaptation to the dairy environment has been extensively studied and documented. However, little is known about their adaptation to the soy environment. We investigated suitability of soymilk as a substratefor growth and acidification by Lactobacillus delbrueckii CIRM-BIA1592 and Propionibacterium freudenreichii CIRM-BIA129. A specific focus was made on changes in term of proteome composition, cellular morphology in comparison with the cultivation in cow milk. In order to understand such adaptation, we explored their growth and survival in soy milk ultrafiltrate and compared it to cow’s milk ultrafiltrate. The two species grew in cow milk,yet not in soy milk. P. freudenreichii did grow in soy milk when co-cultured with the lactic acid bacterium L. plantarum while L. delbrueckii did not, even in co-culture with S. thermophilus. Our results revealed major differences in bacterial morphology and proteome composition. Lactobacilli upregulated stress proteins, and downregulated cell cycle and division machinery. Regarding propionibacteria, differential expression of proteins involved in amino acid metabolism, in carbohydrate metabolism, and in stress remediation was observed. These results suggest that changing the fermented substrate may significantly affect both technological and probiotic properties of conventional dairy starters. This should be considered for the development of new fermented functional foods. Screening of a large collection of lactic and propionic acid bacteria is in progress, based on their technological and probiotic properties. Bacterial stains and consortia thereof will be selected for the production of new plant-based fermented functional foods
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- 2022
44. Effets protecteurs combinés de bactéries propioniques laitières et des acides gras polyinsaturés n-6 sur la barrière épithéliale intestinale
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Marine, Mantel, beaudeau, julie, Mahe, Maxime, ROSA DO CARMO, Fillipe Luiz, Illikoud, Nassima, Cenac, Nicolas, Jan, Gwénaël, Rolli-Derkinderen, Malvyne, Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Claude Bernard Lyon 1 - Faculté des sciences et technologies (UCBL FST), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, The Enteric Nervous System in gut and brain disorders [U1235] (TENS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Physiopathology Of Gut Brain Axis, Digestive health research institute, INSERM U1220, Université de Nantes, (CNRS dans l'institut du Thorax) Institut des Maladies de l'Appareil Digestif, réseau, Galactinnov, est porté par Pierre Germon (INRA, UMR1282 Infectiologie et Santé Publique-ISP, Nouzilly)., Galactinnov, Giboulot, Anne, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Inserm CRCN UMR_S 1235 The Enteric Nervous System In Gut And Brain Disorders, and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST
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propionibacterium freudenreichi ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,acides gras polyinsaturés oméga-6 ,barrière épithéliale intestinale ,colite ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,inflammation de l'intestin - Abstract
International audience; L’intégrité de la barrière épithéliale intestinale (BEI) joue un rôle central dans la pathogenèse des maladies inflammatoires chroniques de l’intestin (MICI). Un intérêt grandissant est porté aux composés alimentaires capables de renforcer ou de restaurer la BEI. C’est le cas de souches de Propionibacterium freudenreichii, une bactérie propionique laitière, capable de restaurer la BEI fragilisée dans un contexte inflammatoire. Egalement, il existe une protection locale de la BEI exercée par les produits du métabolisme des acides gras polyinsaturés oméga-6 (AGPI n-6) par les cellules intestinales. Les omégas 6 sont apportés par l’alimentation, dont le lait. Les enzymes de leur métabolisme sont sous-exprimés chez les patients atteints de MICI, empêchant l’action bénéfique d’une supplémentation en AGPI n-6 chez ces patients. L’augmentation de la biodisponibilité des dérivés AGPI n-6 reste une piste intéressante. Par ailleurs, certaines souches de P. freudenreichii, sont capables de convertir l’acide linoléique, un AGPI n-6, en acide linoléique conjugué (ALC), capable de prévenir des colites induites chez des souris. Ces souches pourraient donc moduler la biodisponibilité et le métabolisme des AGPI n-6. Notre objectif vise à rechercher des effets additifs voire synergiques entre l’effet de 1) P. freudenreichii et 2) des AGPI n-6 sur la BEI. Un criblage de trente-trois souches de P. freudenreichii a été réalisé basé sur les critères suivants : 1) caractérisation de la capacité à induire la production de cytokines anti-inflammatoire (IL-10) par stimulation de PBMCs humaines 2) identification de protéines de surface (S-layer protein) responsable des propriétés anti-inflammatoire par une technique de rasage enzymatique suivis d’une identification par MS/MS 3) mesure de l’activité lipolytique dans la matière grasse laitière et de la conversion de l’acide linoléique en ALC avec une identification des lipides par GC-MS. Toutes les souches sont capables d’induire la production d’IL-10. Seulement, la puissance d’induction d’IL-10 est souche dépendante. Sur les trente-trois souches, dix présentent un profil S-layer anti-inflammatoire. Toutes les souches sont capables de lipolyser la matière grasse laitière, certaines produisent de l’acide linoléique. Toutes les souches exceptées une, sont capable de convertir l’acide linoléique en ALC. De façon similaire aux profils des cytokines, la production d’ALC est souche dépendante. Ces résultats apportent de nouvelles connaissances sur P. freudenreichii, et vont permettre sélectionner des souches qui, ayant un potentiel anti-inflammatoire ainsi que pro-réparateur, seront testées in vivo pour réduire la colite, avec ou sans supplémentation en AGPI n-6.
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- 2021
45. SlpB Protein Enhances the Probiotic Potential of L. lactis NCDO 2118 in Colitis Mice Model
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Belo, Giovanna A., primary, Cordeiro, Bárbara F., additional, Oliveira, Emiliano R., additional, Braga, Marina P., additional, da Silva, Sara H., additional, Costa, Bruno G., additional, Martins, Flaviano dos S., additional, Jan, Gwénaël, additional, Le Loir, Yves, additional, Gala-García, Alfonso, additional, Ferreira, Enio, additional, Azevedo, Vasco, additional, and do Carmo, Fillipe L. R., additional
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- 2021
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46. Lyophilized Symbiotic Mitigates Mucositis Induced by 5-Fluorouracil
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Savassi, Bruna, primary, Cordeiro, Bárbara F., additional, Silva, Sara H., additional, Oliveira, Emiliano R., additional, Belo, Giovanna, additional, Figueiroa, Alessandra Gomes, additional, Alves Queiroz, Maria Izabel, additional, Faria, Ana Maria Caetano, additional, Alves, Juliana, additional, Silva, Tales Fernando da, additional, Campos, Gabriela Munis, additional, Esmerino, Erick A., additional, Rocha, Ramon S., additional, Freitas, Monica Q., additional, Silva, Marcia C., additional, Cruz, Adriano G., additional, Vital, Kátia Duarte, additional, Fernandes, Simone O.A., additional, Cardoso, Valbert N., additional, Acurcio, Leonardo Borges, additional, Jan, Gwénaël, additional, Le Loir, Yves, additional, Gala-Garcia, Alfonso, additional, do Carmo, Fillipe Luiz R., additional, and Azevedo, Vasco, additional
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- 2021
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47. Assembling immunomodulatory strains of Propionibacterium freudenreichii, Lactobacillus delbrueckii and Streptococcus thermophilus to produce an anti-inflammatory Emmental cheese
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Rosa Do Carmo, Fillipe Luiz, de Oliveira Carvalho, Rodrigo Dias, da Silva, Sara Heloisa, Oliveira, Emiliano Rosa, Cara, Denise Carmona, Faria, Ana Maria Caetano, Rabah, Houem, Luiz, Fillipe, Do Carmo, Rosa, Dias, Rodrigo, Carvalho, Oliveira, Fernandes Cordeiro, Bárbara, Heloisa da Silva, Sara, Oliveira, Emiliano, Lemos, Luisa, Cara, Denise, Caetano Faria, Ana, Garric, Gilles, Harel-Oger, Marielle, Le Loir, Yves, Azevedo, Vasco, Bouguen, Guillaume, Foligne, Benoit, Jan, Gwénaël, Universidade Federal de Minas Gerais [Belo Horizonte] (UFMG), I.M. Sechenov First Moscow State Medical University (Sechenov University), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Pôle Agronomique Ouest, Régions Bretagne et Pays de la Loire F-35 042 Rennes, Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Departamento de Genética, Ecologia e evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte Minas Gerais CP 486 CEP 31270-901 , Brazil, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), and Giboulot, Anne
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colitis ,Inflammatory Bowel Disease ,food and beverages ,immunomodulation ,cheese ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,inflammation ,Propionibacteria ,Emmental ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,intestine ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,probiotic - Abstract
International audience; Introduction and Aims. Inflammatory Bowel Diseases (IBD), including Ulcerative Colitis (UC), coincide with alterations in the gut microbiota. Consumption of immunomodulatory strains of probiotic bacteria may induce or prolong remission in UC patients. Fermented foods, including cheeses, constitute major vectors for bacteria consumption. New evidences revealed anti-inflammatory effects of selected strains within the species P.freudenreichii, S.thermophilus and L.delbrueckii. We thus hypothesized that consumption of a functional cheese, fermented by selected strains of these three species, may exert a positive effect on IBD.Methods. Strains of each bacterial species were screened based on the ability to induce regulatory IL-10 in human immune PBMC cells, or to downregulate nuclear factor kappa B (NF-κB) activation in a cultured HT-29 human intestinal epithelial cell line. We then investigated the effect of monostrain experimental cheese. We further investigated the impact two-strains experimental cheese. Finally, we produced in industrial conditions an Emmental cheese using one selected strain of each of the three species, i.e. P. freudenreichii CIRM-BIA 129 in combination with Lactobacillus delbrueckii CNRZ327 and Streptococcus thermophilus LMD-9. Consumption of all cheeses was investigated with respect to prevention of chemically induced colitis in mice.Results. Consumption of the experimental cheeses, or of the industrial Emmental, reduced the severity of subsequent chemically induced colitis, weight loss, disease activity index and histological score, in mice. Furtherrmore, consumption of the Emmental cheese, in a preventive way, reduced small bowel Immunoglobulin A (IgA) secretion, restored occludin gene expression and prevented induction of Tumor Necrosis Factor α (TNFα), Interferon γ (IFNγ) and Interleukin-17 (IL-17).Conclusion. Assembling immunomodulatory strains of both lactic acid and propionic acid starter bacteria leads to an anti-inflammatory Emmental cheese, as revealed in vivo. This opens new perspectives for the development of functional fermented food products for personalised nutrition in the context of IBD.
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- 2021
48. Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: Reverse engineering development of an anti-inflammatory cheese
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Plé, Coline, Breton, Jérôme, Richoux, Romain, Nurdin, Marine, Deutsch, Stéphanie-Marie, Falentin, Hélène, Hervé, Christophe, Chuat, Victoria, Lemée, Riwanon, Maguin, Emmanuelle, Jan, Gwénaël, Van de Guchte, Maarten, and Foligné, Benoit
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- 2016
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49. Therapeutic Effects of Probiotic Minas Frescal Cheese on the Attenuation of Ulcerative Colitis in a Murine Model
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Cordeiro, Barbara Fernandes, Alves, Juliana de Lima, Angeli Belo, Giovanna, Oliveira, Emiliano Rosa, Braga, Marina P., Lemos, Luisa, Guimaraes, Jonas T., Silva, Ramon Lima, Rocha, Ramon S., Jan, Gwénaël, Silva, Marcia C., Freitas, Mônica Q., Esmerino, Erik A., Gala Garcia, Alfonso, Ferreira, Ennio, Faria, Anna Maria C., Cruz, Adriano G., Azevedo, Vasco, Rosa Do Carmo, Fillipe Ruiz, Instituto de Ciencias Biologicas [Minas Gerais], Universidade Federal de Minas Gerais [Belo Horizonte] (UFMG), Massachusetts General Hospital, Harvard Medical School [Boston] (HMS), Fluminense Federal University [Niterói], Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and This work was supported by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
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functional food ,cheese ,Lactococcus lactis ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,colitis ,inflammatory bowel disease ,[SDV]Life Sciences [q-bio] ,digestive system diseases ,probiotic ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; Inflammatory bowel diseases (IBDs) constitute disturbances of gastrointestinal tract that cause irreversible changes in the structure and function of tissues. Ulcerative colitis (UC), the most frequent IBD in the population, is characterized by prominent inflammation of the human colon. Functional foods containing probiotic bacteria have been studied as adjuvants to the treatment or prevention of IBDs. The selected probiotic strain Lactococcus lactis NCDO 2118 (L. lactis NCDO 2118) exhibits immunomodulatory effects, with promising results in UC mouse model induced by dextran sodium sulfate (DSS). Additionally, cheese is a dairy food that presents high nutritional value, besides being a good delivery system that can be used to improve survival and enhance the therapeutic effects of probiotic bacteria in the host. Therefore, this work investigated the probiotic therapeutic effects of an experimental Minas Frescal cheese containing L. lactis NCDO 2118 in DSS-induced colitis in mice. During colitis induction, mice that consumed the probiotic cheese exhibited reduced in the severity of colitis, with attenuated weight loss, lower disease activity index, limited shortening of the colon length, and reduced histopathological score. Moreover, probiotic cheese administration increased gene expression of tight junctions’ proteins zo-1, zo-2, ocln, and cln-1 in the colon and increase IL-10 release in the spleen and lymph nodes. In this way, this work demonstrates that consumption of probiotic Minas Frescal cheese, containing L. lactis NCDO 2118, prevents the inflammatory process during DSS-induced colitis in mice, opening perspectives for the development of new probiotic functional foods for personalized nutrition in the context of IBD.
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- 2021
50. A Unique Enhancement of Propionibacterium freudenreichii 's Ability to Remove Pb(II) from Aqueous Solution by Tween 80 Treatment.
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George, Fanny, Titécat, Marie, Barois, Nicolas, Daniel, Catherine, Garat, Anne, Jan, Gwénaël, and Foligné, Benoît
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AQUEOUS solutions ,PROPIONIBACTERIUM ,LEAD abatement ,POLYSORBATE 80 ,BIOREMEDIATION - Abstract
Microbial agents have promise for the bioremediation of Pb(II)-polluted environments and wastewater, the biodecontamination of foods, and the alleviation of toxicity in living organisms. The dairy bacterium Propionibacterium freudenreichii is poorly able to remove Pb(II) from aqueous solution at 25 ppm, ranging from 0 to 10% of initial concentration. Here, we report on an original strong enhancement of this activity (ranging from 75% to 93%, p < 0.01) following the addition of a polysorbate detergent (Tween
® 80) during or either shortly after the growth of a P. freudenreichii culture. We evaluated the optimal Tween® 80 concentration for pretreatment conditions, documented the role of other detergents, and explored the possible mechanisms involved. Our results reveal a novel, environmentally friendly, low-cost pretreatment procedure for enhancing the selective removal of lead from water by probiotic-documented bacteria. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
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