Search

Your search keyword '"Janakiraman Vani"' showing total 48 results
Start Over Author "Janakiraman Vani"
48 results on '"Janakiraman Vani"'

1. TLR 9 activation in dendritic cells enhances salmonella killing and antigen presentation via involvement of the reactive oxygen species.

Author

Amit Lahiri, Ayan Lahiri, Priyanka Das, Janakiraman Vani, M S Shaila, and Dipshikha Chakravortty

Subjects
Medicine, Science
Abstract

Synthetic CpG containing oligodeoxynucleotide Toll like receptor-9 agonist (CpG DNA) activates innate immunity and can stimulate antigen presentation against numerous intracellular pathogens. It was observed that Salmonella Typhimurium growth can be inhibited by the CpG DNA treatment in the murine dendritic cells. This inhibitory effect was mediated by an increased reactive oxygen species production. In addition, it was noted that CpG DNA treatment of dendritic cells during Salmonella infection leads to an increased antigen presentation. Further this increased antigen presentation was dependent on the enhanced reactive oxygen species production elicited by Toll like receptor-9 activation. With the help of an exogenous antigen it was shown that Salmonella antigen could also be cross-presented in a better way by CpG induction. These data collectively indicate that CpG DNA enhance the ability of murine dendritic cells to contain the growth of virulent Salmonella through reactive oxygen species dependent killing.

Published
2010
Full Text
View/download PDF

3. Toward the discovery of vaccine adjuvants: coupling in silico screening and in vitro analysis of antagonist binding to human and mouse CCR4 receptors.

Author

Matthew N Davies, Jagadeesh Bayry, Elma Z Tchilian, Janakiraman Vani, Melkote S Shaila, Emily K Forbes, Simon J Draper, Peter C L Beverley, David F Tough, and Darren R Flower

Subjects
Medicine, Science
Abstract

BACKGROUND:Adjuvants enhance or modify an immune response that is made to an antigen. An antagonist of the chemokine CCR4 receptor can display adjuvant-like properties by diminishing the ability of CD4+CD25+ regulatory T cells (Tregs) to down-regulate immune responses. METHODOLOGY:Here, we have used protein modelling to create a plausible chemokine receptor model with the aim of using virtual screening to identify potential small molecule chemokine antagonists. A combination of homology modelling and molecular docking was used to create a model of the CCR4 receptor in order to investigate potential lead compounds that display antagonistic properties. Three-dimensional structure-based virtual screening of the CCR4 receptor identified 116 small molecules that were calculated to have a high affinity for the receptor; these were tested experimentally for CCR4 antagonism. Fifteen of these small molecules were shown to inhibit specifically CCR4-mediated cell migration, including that of CCR4(+) Tregs. SIGNIFICANCE:Our CCR4 antagonists act as adjuvants augmenting human T cell proliferation in an in vitro immune response model and compound SP50 increases T cell and antibody responses in vivo when combined with vaccine antigens of Mycobacterium tuberculosis and Plasmodium yoelii in mice.

Published
2009
Full Text
View/download PDF

6. A wash-free, dip-type fiber optic plasmonic (DiP) assay for sub-zeptomole analyte detection

Author

Ramakrishna, Bandaru, Janakiraman, Vani, and Sai, V V R

Subjects
Physics - Applied Physics, Physics - Biological Physics
Abstract

This study demonstrates a rapid, wash-free dip-type fiber optic plasmonic sandwich (DiP) assay capable of zeptomole analyte detection and 6-orders of wide dynamic range. The DiP assay is realized on a compact U-bent fiber optic probe surface by dipping the antibody functionalized probe into a mixture of sample solution and AuNP labeled reagent. U-bent fiberoptic probes with a high evanescent wave absorbance sensitivity allow detection of the high extinction gold nanoparticle (AuNP) labels in terms of a drop in the light intensity, which is measured with the help of a pair of LED and photodetector (PD). This simple and low-cost DiP assay gave rise to unprecedented detection limit down to 0.17 zeptomole of human immunoglobulin G (HIgG) in 0.025 ml buffer solution within an assay time of 25 min. Further, silver enhancement of AuNP labels over 5 min resulted in a limit of quantitation (LoQ) down to 0.17 zeptomole (~100 molecules in 0.025 ml). The DiP assay with high sensitivity and low detection limits within an assay time of 30 min demonstrated here could facilitate low cost point-of-care diagnostics as well as high-throughput systems.

Published
2018

20. Elucidating important structural features for the binding affinity of spike ‐ SARS‐CoV‐2 neutralizing antibody complexes.

Author

Sharma, Divya, Rawat, Puneet, Janakiraman, Vani, and Gromiha, M. Michael

Abstract

The coronavirus disease 2019 (COVID‐19) has affected the lives of millions of people around the world. In an effort to develop therapeutic interventions and control the pandemic, scientists have isolated several neutralizing antibodies against SARS‐CoV‐2 from the vaccinated and convalescent individuals. These antibodies can be explored further to understand SARS‐CoV‐2 specific antigen–antibody interactions and biophysical parameters related to binding affinity, which can be utilized to engineer more potent antibodies for current and emerging SARS‐CoV‐2 variants. In the present study, we have analyzed the interface between spike protein of SARS‐CoV‐2 and neutralizing antibodies in terms of amino acid residue propensity, pair preference, and atomic interaction energy. We observed that Tyr residues containing contacts are highly preferred and energetically favorable at the interface of spike protein–antibody complexes. We have also developed a regression model to relate the experimental binding affinity for antibodies using structural features, which showed a correlation of 0.93. Moreover, several mutations at the spike protein–antibody interface were identified, which may lead to immune escape (epitope residues) and improved affinity (paratope residues) in current/emerging variants. Overall, the work provides insights into spike protein–antibody interactions, structural parameters related to binding affinity and mutational effects on binding affinity change, which can be helpful to develop better therapeutics against COVID‐19. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

23. Dendritic Cells in Autoimmune Diseases

Author

Mohan S. Maddur, Jordan D. Dimitrov, Sébastien Lacroix-Desmazes, Jagadeesh Bayry, Srini V. Kaveri, Kithiganahalli Narayanaswamy Balaji, and Janakiraman Vani

Subjects
medicine.medical_specialty, Systemic lupus erythematosus, business.industry, chemical and pharmacologic phenomena, medicine.disease, medicine.disease_cause, Rheumatology, Autoimmunity, Immune tolerance, Intracellular signal transduction, Immune system, Co-stimulation, Internal medicine, Immunology, Medicine, business, Antigen-presenting cell
Abstract

Dendritic cells (DCs) are professional antigen presenting cells, which play a crucial role both in maintaining immune tolerance and in inducing adaptive immune responses. Therefore, DCs are the most sought in inciting autoimmu- nity and its sustenance to autoimmune diseases. The emerging knowledge of the importance of DCs, DC-derived cytoki- nes, and intracellular signal transduction pathways in mediating autoimmune diseases and in creating an inflammatory en- vironment provides a rationale for pursuing strategies to block these inflammatory pathways for therapeutic purposes.

Published
2010

25. Immunization of mice with DNA coding for the variable regions of anti-idiotypic antibody generates antigen-specific response

Author

R. Nayak, Janakiraman Vani, and Melkote S. Shaila

Subjects
Male, T-Lymphocytes, T cell, Biology, Immunoglobulin light chain, Major histocompatibility complex, Antibodies, Immunoglobulin G, Epitope, Cell Line, Epitopes, Mice, Antigen, Antibody Specificity, Escherichia coli, Vaccines, DNA, medicine, Animals, Cloning, Molecular, Cell Proliferation, Microbiology & Cell Biology, B-Lymphocytes, Mice, Inbred BALB C, General Veterinary, General Immunology and Microbiology, Reverse Transcriptase Polymerase Chain Reaction, T-cell receptor, Public Health, Environmental and Occupational Health, Antibodies, Monoclonal, DNA, Flow Cytometry, Recombinant Proteins, Hemagglutinins, Infectious Diseases, medicine.anatomical_structure, Antibody Formation, Immunology, biology.protein, Cytokines, Molecular Medicine, Immunization, Antibody, Immunologic Memory, T-Lymphocytes, Cytotoxic
Abstract

Understanding the mechanisms of generation and maintenance of immunological memory is crucial for rational vaccine design.Ahypothesis known as relay hypothesis was earlier proposed which explains the maintenance of immunological memory through interaction of idiotypic and anti-idiotypic lymphocytes. In the present study, we have shown that immunization with rinderpest virus hemagglutinin protein specific anti-idiotypic antibody $(Ab2v_\beta)$ DNAs coding for heavy and light chains generates antigen-specific antibody and T cell responses as well as Ab1 specific T cell response. We further show that boosting with the recombinant $Ab_2-v_\beta$ proteins generates B and T cell memory response specific for antigen in anti-id DNA primed mice. This study provides experimental evidence for perpetuation of immunological memory through idiotypic network interactions.

Published
2007

26. GM-CSF along with IL-4 but not alone is indispensable for the differentiation of human dendritic cells from monocytes

Author

Janakiraman Vani, Sahana Holla, Meenu Sharma, Srini V. Kaveri, Jagadeesh Bayry, Kithiganahalli Narayanaswamy Balaji, Indian Institute of Science [Bangalore] (IISc Bangalore), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), École pratique des hautes études (EPHE), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bayry, Jagadeesh, and Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)

Subjects
Microbiology & Cell Biology, Immunology, Dendritic Cells, Th1 Cells, Biology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Cell biology, Th2 Cells, Hypersensitivity, Animals, Immunology and Allergy, Interleukin-4, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, Interleukin 4, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

27. Mycobacterium tuberculosis cell wall-associated Rv3812 protein induces strong dendritic cell-mediated interferon γ responses and exhibits vaccine potential

Author

Melukote S. Shaila, Srini V. Kaveri, Janakiraman Vani, Jagadeesh Bayry, Kithiganahalli Narayanaswamy Balaji, Jamma Trinath, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Indian Institute of Science, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), École pratique des hautes études (EPHE), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bayry, Jagadeesh, and Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)

Subjects
Biology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Microbiology, law.invention, Cell wall, Bacterial protein, Mycobacterium tuberculosis, 03 medical and health sciences, Interferon-gamma, 0302 clinical medicine, Interferon γ, Bacterial Proteins, law, Cell Wall, medicine, Immunology and Allergy, Humans, Interferon gamma, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Tuberculosis Vaccines, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Dendritic cell, Dendritic Cells, biology.organism_classification, Virology, Recombinant Proteins, Toll-Like Receptor 2, 3. Good health, Infectious Diseases, Recombinant DNA, Cytokines, Tuberculosis vaccines, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, 030215 immunology, medicine.drug
Abstract

International audience

Published
2013

28. Impact of gp120 on Dendritic Cell-Derived Chemokines: Relevance for the Efficacy of gp120-Based Vaccines for HIV-1

Author

Meenu Sharma, Srini V. Kaveri, Janakiraman Vani, Melkote S. Shaila, Jagadeesh Bayry, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bayry, Jagadeesh, École Pratique des Hautes Études (EPHE), and Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE)

Subjects
Microbiology (medical), Chemokine, Clinical Biochemistry, Immunology, Human immunodeficiency virus (HIV), HIV Infections, Biology, Vaccine antigen, HIV Envelope Protein gp120, medicine.disease_cause, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Immunology and Allergy, Humans, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Letter to the Editor, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, AIDS Vaccines, 0303 health sciences, Dendritic cell, Dendritic Cells, biochemical phenomena, metabolism, and nutrition, Virology, 3. Good health, biology.protein, Chemokines, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, 030215 immunology
Abstract

Dendritic cells (DC) are the professional antigen-presenting cells and act as sentinels of the immune system. Therefore, the activation status of DC upon an encounter with vaccine antigens determines the intensity and duration of protective immune responses. DC are also critical for the transmission

Published
2012

29. Myeloid dendritic cell dysfunction during primary HIV-1 infection is independent of interaction with gp120

Author

Janakiraman Vani, Srini V. Kaveri, and Jagadeesh Bayry

Subjects
Primary (chemistry), business.industry, Myeloid Dendritic Cell, Human immunodeficiency virus (HIV), HIV Infections, Dendritic Cells, HIV Envelope Protein gp120, medicine.disease_cause, Infectious Diseases, Text mining, Immunology, Host-Pathogen Interactions, medicine, HIV-1, Immunology and Allergy, Animals, Humans, business
Published
2012

30. Inhibition of differentiation, amplification, and function of human TH17 cells by intravenous immunoglobulin

Author

Mohan S. Maddur, Pushpa Hegde, Janakiraman Vani, Srini V. Kaveri, Sébastien Lacroix-Desmazes, Jagadeesh Bayry, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Bayry, Jagadeesh, Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), École Pratique des Hautes Études (EPHE), and Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE)

Subjects
Immunology, Biology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, 03 medical and health sciences, Interleukin 21, 0302 clinical medicine, Humans, Immunology and Allergy, Cytotoxic T cell, IL-2 receptor, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Antigen-presenting cell, STAT4, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, Cell Proliferation, 030304 developmental biology, Interleukin 3, 0303 health sciences, ZAP70, Immunoglobulins, Intravenous, Cell Differentiation, Natural killer T cell, 3. Good health, Cytokines, Th17 Cells, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, Signal Transduction, 030215 immunology
Abstract

Background T H 17 cells play a critical role in the pathogenesis of several autoimmune and allergic diseases. Intravenous immunoglobulin (IVIg), a therapeutic preparation of polyclonal IgG that is increasingly used in the treatment of diverse autoimmune and allergic diseases, might target T H 17 cells to exert therapeutic effects. Objective We sought to examine whether IVIg interferes with the development and function of human T H 17 cells. Methods T H 17 cells were differentiated from naive human CD4 + T cells in the presence of TGF-β and IL-21. T H 17 cells were amplified by stimulating memory CD4 + T cells in the presence of IL-1β and IL-6. The effect of IVIg was examined on the differentiation and amplification of T H 17 cells, expression of the T H 17 lineage-specific transcription factor retinoic acid-related orphan receptor C, secretion of T H 17 effector cytokines, and phosphorylation of signal transducer and activator of transcription 3, a transcription factor that plays an important role in T H 17 cell development and function. Results IVIg inhibits the differentiation and amplification of human T H 17 cells, as well as the production of their effector cytokines IL-17A, IL-17F, IL-21, and CCL20. The inhibitory effects of IVIg on T H 17 cells are F(ab′) 2 dependent and involve interference with the expression of retinoic acid-related orphan receptor C and activation of signal transducer and activator of transcription 3. Also, IVIg significantly enhanced forkhead box protein 3–positive regulatory T cells among the memory CD4 + T cells. Conclusion These results reveal a novel mechanism of action of IVIg in achieving a therapeutic effect in autoimmune and allergic diseases, in which T H 17 cells play a key modulatory role in sustaining the chronic inflammatory response. Our results also suggest a reciprocal regulation of T H 17 and regulatory T-cell populations by IVIg.

Published
2011

31. Intravenous immunoglobulin induces proliferation and immunoglobulin synthesis from B cells of pathients wit common variable immunodeficiency: a mechanism underlying the beneficial effect of IVIg in primary immunodeficiencies

Author

Olivier Hermine, Bharath Wootla, Luc Mouthon, Yoann Crabol, Janakiraman Vani, Sophie Sibéril, Jordan D. Dimitrov, Yves Levy, Sébastien Lacroix-Desmazes, Jagadeesh Bayry, Emilie M. Fournier, Mikael Berdah, Eric Oksenhendler, Srini V. Kaveri, Catherine Sautès-Fridman, Mohan S. Maddur, Slama, Catherine, Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), Architecture et Réactivité de l'ARN (ARN), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Department of Neurology, Mayo Clinic Comprehensive Cancer Center, Mayo Clinic, CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'Immunopathologie Clinique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Supported by grants from Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Université Paris Descartes and Laboratoire Français du Fractionnement et des Biotechnologies, France. France IVIg (Tegeline®) is a kind gift from Laboratoire Français du Fractionnement et des Biotechnologies, France., Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), École Pratique des Hautes Études (EPHE), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Department of Structural Biology, Weizmann Institute, Cytokines, hématopoïèse et réponse immune (CHRI), Bayry, Jagadeesh, Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), Université de Lyon - Université de Lyon - Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de référence français pour les maladies auto-immunes et systémiques rares [Hôpital Pitié-Salpêtrière - APHP], Université Pierre et Marie Curie - Paris 6 (UPMC)-CHU Pitié-Salpêtrière [APHP], and Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)

Subjects
Adult, Male, medicine.medical_treatment, Immunology, Cell Separation, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Flow cytometry, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Immune system, hemic and lymphatic diseases, medicine, Immunology and Allergy, Humans, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Transcription factor, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, B cell, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Aged, Cell Proliferation, 0303 health sciences, B-Lymphocytes, biology, medicine.diagnostic_test, Effector, business.industry, Common variable immunodeficiency, Immunoglobulins, Intravenous, Middle Aged, medicine.disease, Flow Cytometry, 3. Good health, Cytokine, medicine.anatomical_structure, Common Variable Immunodeficiency, Antibody Formation, biology.protein, Female, Antibody, business, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, 030215 immunology
Abstract

International audience; Common variable immunodeficiency (CVID) is associated with low serum immunoglobulin concentrations and an increased susceptibility to infections and autoimmune diseases. The treatment of choice for CVID patients is replacement intravenous immunoglobulin (IVIg) therapy. IVIg has been beneficial in preventing or alleviating the severity of infections and autoimmune and inflammatory process in majority of CVID patients. Although the mechanisms of action of IVIg given as 'therapeutic high dose' in patients with autoimmune diseases are well studied, the underlying mechanisms of beneficial effects of IVIg in primary immunodeficiencies are not completely understood. Therefore we investigated the effect of 'replacement dose' of IVIg by probing its action on B cells from CVID patients. We demonstrate that IVIg at low doses induces proliferation and immunoglobulin synthesis from B cells of CVID patients. Interestingly, B cell stimulation by IVIg is not associated with induction of B cell effector cytokine IFN-γ and of transcription factor T-bet. Together, our results indicate that in some CVID patients, IVIg rectifies the defective signaling of B cells normally provided by T cells and delivers T-independent signaling for B cells to proliferate. IVIg 'replacement therapy' in primary immunodeficiencies is therefore not a merepassive transfer of antibodies to prevent exclusively the recurrent infections; rather it has an active role in regulating autoimmune and inflammatory responses through modulating B cell functions and thus imposing dynamic equilibrium of the immune system.

Published
2011

32. Autoimmunity as a Predisposition for Infectious Diseases

Author

Sébastien Lacroix-Desmazes, Janakiraman Vani, Jagadeesh Bayry, Srinivas V. Kaveri, and Mohan S. Maddur

Subjects
lcsh:Immunologic diseases. Allergy, Opinion, Immunology, Inflammation, Autoimmunity, Biology, medicine.disease_cause, Microbiology, Communicable Diseases, Pathogenesis, Immune system, Virology, Genetics, medicine, Humans, Genetic Predisposition to Disease, Molecular Biology, Pathological, lcsh:QH301-705.5, Cytokine Therapy, Autoantibody, Infectious Diseases, lcsh:Biology (General), biology.protein, Parasitology, Antibody, medicine.symptom, lcsh:RC581-607
Abstract

Autoimmunity refers to an inappropriate immune response against self-components of the host that results in pathological conditions. Autoimmune diseases are characterized by an activation of autoreactive T and B cells, are associated in some cases with the production of pathogenic autoantibodies against self-molecules, culminating in inflammation and tissue damage. The reasons for the breakdown of tolerance mechanisms leading to autoimmunity are not clearly known. However, a combination of genetic, immunological, and environmental factors plays a critical role in the pathogenesis of autoimmunity [1]–[5].

Published
2010

33. TLR 9 Activation in Dendritic Cells Enhances Salmonella Killing and Antigen Presentation via Involvement of the Reactive Oxygen Species

Author

M. S. Shaila, Priyanka Das, Janakiraman Vani, Ayan Lahiri, Amit Lahiri, and Dipshikha Chakravortty

Subjects
Science, Antigen presentation, Microbiology/Innate Immunity, Biology, Microbiology, Infectious Diseases/Bacterial Infections, Antigen, Salmonella, Immunology/Immunity to Infections, Cytotoxic T cell, Humans, Antigen-presenting cell, Microbiology & Cell Biology, Antigens, Bacterial, Multidisciplinary, Innate immune system, Increased reactive oxygen species production, Dendritic Cells, Toll-Like Receptor 9, CpG site, Others, Medicine, CpG Islands, Reactive Oxygen Species, Research Article
Abstract

Synthetic CpG containing oligodeoxynucleotide Toll like receptor-9 agonist (CpG DNA) activates innate immunity and can stimulate antigen presentation against numerous intracellular pathogens. It was observed that Salmonella Typhimurium growth can be inhibited by the CpG DNA treatment in the murine dendritic cells. This inhibitory effect was mediated by an increased reactive oxygen species production. In addition, it was noted that CpG DNA treatment of dendritic cells during Salmonella infection leads to an increased antigen presentation. Further this increased antigen presentation was dependent on the enhanced reactive oxygen species production elicited by Toll like receptor-9 activation. With the help of an exogenous antigen it was shown that Salmonella antigen could also be cross-presented in a better way by CpG induction. These data collectively indicate that CpG DNA enhance the ability of murine dendritic cells to contain the growth of virulent Salmonella through reactive oxygen species dependent killing.

Published
2010

34. Contribution of myeloid dendritic cells to type I interferon-induced cytokines and chemokines: comment on the article by Bilgic et al

Author

Mohan S, Maddur, Janakiraman, Vani, Sébastien, Lacroix-Desmazes, Srini V, Kaveri, and Jagadeesh, Bayry

Subjects
Interferon Type I, Gene Expression, Humans, RNA-Binding Proteins, Bone Marrow Cells, Dendritic Cells, Chemokines, Carrier Proteins, Biomarkers, Cells, Cultured, Dermatomyositis, Adaptor Proteins, Signal Transducing, Autoimmune Diseases
Published
2010

35. Immunomodulation by Intravenous Immunoglobulin: Role of Regulatory T Cells

Author

Pushpa Hegde, Srini V. Kaveri, Shivashankar Othy, Janakiraman Vani, Mohan S. Maddur, Sébastien Lacroix-Desmazes, Jagadeesh Bayry, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Bayry, Jagadeesh, École Pratique des Hautes Études (EPHE), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Encephalomyelitis, Autoimmune, Experimental, Immunology, chemical and pharmacologic phenomena, Inflammation, T-Lymphocytes, Regulatory, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Autoimmune Diseases, 03 medical and health sciences, Mice, 0302 clinical medicine, hemic and lymphatic diseases, medicine, Immunology and Allergy, Animals, Humans, Immunologic Factors, In patient, Immune homeostasis, IL-2 receptor, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Mode of action, Beneficial effects, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Immunity, Cellular, biology, business.industry, Models, Immunological, FOXP3, Immunoglobulins, Intravenous, hemic and immune systems, Adoptive Transfer, 3. Good health, biology.protein, medicine.symptom, Antibody, business, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, 030215 immunology
Abstract

An altered immune homeostasis as a result of deficiency or defective function of CD4(+)CD25(+)FoxP3(+) regulatory T cells (Tregs) is common in several autoimmune diseases. Hence, therapeutic strategies to render Tregs functionally competent are being investigated. Intravenous immunoglobulin (IVIG) is being increasingly used for the treatment of a wide range of autoimmune and inflammatory diseases. Recent studies have demonstrated that IVIG induces the expansion of Tregs and enhances their suppressive functions. These effects of IVIG on Tregs correlate with the beneficial effects of IVIG in patients with autoimmune diseases. Thus, modulation of Tregs by IVIG represents a novel mode of action that explains the therapeutic effects of IVIG in T cell-mediated autoimmune diseases. However, the molecular mechanisms involved in IVIG-mediated modulation of Tregs are unclear and need further investigation.

Published
2010

36. Contribution of myeloid dendritic cells to type I interferon-induced cytokines and chemokines

Author

Janakiraman Vani, Srini V. Kaveri, Mohan S. Maddur, Sébastien Lacroix-Desmazes, and Jagadeesh Bayry

Subjects
Chemokine, Myeloid, biology, business.industry, medicine.medical_treatment, Immunology, Signal transducing adaptor protein, Dendritic cell, medicine.anatomical_structure, Cytokine, Rheumatology, Interferon, medicine, biology.protein, Immunology and Allergy, Pharmacology (medical), business, medicine.drug
Published
2010

37. Parameters that influence the prediction of epidemiological benefits of more-effective tuberculosis vaccines, drugs, and diagnosis

Author

Sébastien Lacroix-Desmazes, Jagadeesh Bayry, Srini V. Kaveri, Mohan S. Maddur, and Janakiraman Vani

Subjects
medicine.medical_specialty, education.field_of_study, Multidisciplinary, Tuberculosis, biology, business.industry, Incidence (epidemiology), Population, medicine.disease, biology.organism_classification, Mycobacterium tuberculosis, Tuberculosis diagnosis, Immunology, Global health, Medicine, business, Intensive care medicine, education, Tuberculosis vaccines, Cause of death
Abstract

Mycobacterium tuberculosis, the etiologic agent of tuberculosis, is a leading cause of death worldwide, killing an estimated 3 million people annually. An estimate of one-third of world's population is infected with tuberculosis bacilli. By using an age-structured mathematical model of tuberculosis, Abu-Raddad et al. (1) recently explored the potential benefits of novel interventions under development and those not yet in the portfolio, focusing on the WHO Southeast Asia region. Their model predicts that a triple combination of a portfolio vaccine, drug regimen, and diagnostics reduces the incidence of tuberculosis by 71%. Although we agree that novel regimens are expected to reduce tuberculosis incidence in the future, for the reasons discussed below, we believe that the proposed reductions in tuberculosis incidence is overestimated. The major drawback with the model is that the authors have considered only the latent infections as a parameter that may determine the sensitivity and uncertainty of the predictions. However, tuberculosis is a very complex disease and, in addition to host–pathogen parameters, one has to consider several socio-economic factors for modeling tuberculosis.

Published
2009

38. B Lymphocytes from Patients with Tuberculosis Exhibit Hampered Antigen‐Specific Responses with Concomitant Overexpression of Interleukin‐8

Author

Janakiraman Vani, Srini V. Kaveri, Uma Maheshwari Krishnaswamy, Melukote S. Shaila, Mohan K.N. Rao, Jagadeesh Bayry, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), École pratique des hautes études (EPHE), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bayry, Jagadeesh, and Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)

Subjects
Chemokine, Tuberculosis, T cell, Inflammation, Biology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Proinflammatory cytokine, Mycobacterium tuberculosis, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Immunology and Allergy, Interleukin 8, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Microbiology & Cell Biology, 0303 health sciences, medicine.disease, biology.organism_classification, 3. Good health, Infectious Diseases, medicine.anatomical_structure, Immunology, biology.protein, medicine.symptom, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, 030215 immunology
Abstract

To the Editor-Tuberculosis due to Mycobacterium tuberculosis is 1 of the 3 major killers among infectious diseases. Deciphering the interactions between M. tuberculosis and the innate and adaptive immune compartments of the host is critical for understanding the pathogenesis of tuberculosis and for designing effective immunotherapeutic interventions. By use of a juvenile rhesus monkey model, Qiu et al recently demonstrated that severe tuberculosis induces unbalanced up-regulation of the immune gene networks of inflammatory cytokines, chemokines, and their receptors, but low levels of T cell responses specific to purified protein derivative [1]. The authors conclude that the overexpression of immune genes after tuberculosis infection favors inflammation and suppression of antigen-specific T cells.

Published
2009

39. Immunointervention for patients with HIV and tuberculosis

Author

Janakiraman Vani, Srini V. Kaveri, Michel D. Kazatchkine, Jagadeesh Bayry, Kushagra Bansal, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Immunopathologie et immunointervention thérapeutique, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR58-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bayry, Jagadeesh, École Pratique des Hautes Études (EPHE), and Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE)

Subjects
Tuberculosis, Human immunodeficiency virus (HIV), HIV Infections, medicine.disease_cause, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, 03 medical and health sciences, 0302 clinical medicine, Immunity, Pandemic, medicine, Humans, 030212 general & internal medicine, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Antigen-presenting cell, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Microbiology & Cell Biology, AIDS Vaccines, 0303 health sciences, Immunity, Cellular, biology, business.industry, Contraindications, medicine.disease, Virology, 3. Good health, Infectious Diseases, Immunology, biology.protein, Therapeutic vaccine, Antibody, business, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, Immune activation
Abstract

Therapeutic options aimed at confronting the HIV pandemic face many obstacles. Current opinion on HIV-induced pathogenic immune activation and strategies aimed at eliminating HIV, including a potential role for non-neutralising antibodies as part of a therapeutic vaccine option, was elegantly reviewed by Martin Cadogan and Angus Dalgleish. 1 It is important to note that, for eliciting such antibody responses in patients, functionally fit antigen presenting cells and effector T and B cells are cruc.

Published
2009

40. Modulation of human dendritic cell maturation and function by natural IgG antibodies

Author

Michel D. Kazatchkine, Marie-Françoise Bloch, Sriramulu Elluru, Sandrine Delignat, Sébastien Lacroix-Desmazes, Jagadeesh Bayry, Janakiraman Vani, Srini V. Kaveri, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunopathologie et immunointervention thérapeutique, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR58-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), GEC UTC (GEC UTC), Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Ministère de l'Europe et des Affaires étrangères (MEAE), Octave, Stephane, Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université de Technologie de Compiègne (UTC)

Subjects
X-linked agammaglobulinemia, [SDV.IMM] Life Sciences [q-bio]/Immunology, T-Lymphocytes, Immunology, Natural IgG antibodies, chemical and pharmacologic phenomena, Dendritic cells, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Immunoglobulin G, Immune tolerance, Common variable immunodeficiency, 03 medical and health sciences, 0302 clinical medicine, Immune system, Agammaglobulinemia, medicine, Humans, Immunology and Allergy, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Intravenous immunoglobulin, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, B-Lymphocytes, 0303 health sciences, biology, Cell Differentiation, Genetic Diseases, X-Linked, Dendritic cell, medicine.disease, Virology, 3. Good health, Immunization, biology.protein, [SDV.IMM]Life Sciences [q-bio]/Immunology, Antibody, 030215 immunology
Abstract

International audience; Dendritic cells (DCs) are professional antigen-presenting cells, which have a central role in the initiation of primary immune responses and in maintaining immune tolerance. The functions of DCs can be regulated both by environmental signals as well as signals delivered by endogenous molecules. Recently we have examined regulation of human DCs by B cells via natural IgG antibodies. Natural antibodies (NAbs) are defined as antibodies that circulate in normal individuals in the absence of deliberate immunization or microbial aggression. We demonstrate that the differentiation of DCs is severely impaired in primary immunodeficient patients such as X-linked agammaglobulinemia (XLA) and common variable immunodeficiency (CVID) at least in part due to the deficiency of circulating NAbs. Further, we show that NAbs are able to restore normal phenotypes of DCs from patients with XLA and CVID. Our results suggest that B cells promote bystander DC development through NAbs and the interaction between NAbs and DCs may play a role in steady-state migration of DCs.

Published
2008

41. Role of natural antibodies in immune homeostasis: IVIg perspective

Author

Michel D. Kazatchkine, Vir Singh Negi, Sriramulu Elluru, Jagadeesh Bayary, Srini V. Kaveri, Janakiraman Vani, Sébastien Lacroix-Desmazes, Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Immunopathologie et immunointervention thérapeutique, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR58-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bayry, Jagadeesh, Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), École pratique des hautes études (EPHE), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Idiotype, T-Lymphocytes, Immunology, Receptors, Cell Surface, Receptors, Fc, Biology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, 03 medical and health sciences, 0302 clinical medicine, Complement Inactivator Proteins, Immunoglobulin Idiotypes, hemic and lymphatic diseases, Immunology and Allergy, Homeostasis, Humans, Immune homeostasis, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Receptor, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Autoantibodies, 0303 health sciences, B-Lymphocytes, Autoantibody, Immunoglobulins, Intravenous, Dendritic Cells, 3. Good health, biology.protein, Cytokines, Antibody, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, 030215 immunology
Abstract

Intravenous immunoglobulin (IVIg) has increasingly been used for the treatment of autoimmune and systemic inflammatory diseases in addition to supportive therapy of immunodeficient patients. Although a considerable progress has been made in understanding the mechanisms by which IVIg exerts immunomodulatory functions in these diseases, they remain not fully elucidated. The mode of action of IVIg is complex, involving interference with activation of complement and the cytokine network, modulation of: idiotype network, expression of Fc receptors, and activation, differentiation and effector functions of T and B cells and of antigen-presenting cells such as dendritic cells. The therapeutic effects of IVIg most likely reflect the functions of natural antibodies in maintaining immune homeostasis in healthy individuals.

Published
2008

42. Maintenance of antigen-specific immunological memory through variable regions of heavy and light chains of anti-idiotypic antibody

Author

R. Nayak, Melkote S. Shaila, and Janakiraman Vani

Subjects
medicine.drug_class, Immunology, Molecular Sequence Data, Immunoglobulin Variable Region, Epitopes, T-Lymphocyte, Hemagglutinins, Viral, Immunoglobulin light chain, Monoclonal antibody, Binding, Competitive, Cell Line, Rats, Sprague-Dawley, Mice, Viral Proteins, Immune system, Antigen, Species Specificity, Splenocyte, medicine, Immunology and Allergy, Animals, Amino Acid Sequence, Cell Proliferation, Glycoproteins, Microbiology & Cell Biology, B-Lymphocytes, Mice, Inbred BALB C, biology, Original Articles, In vitro, Hypervariable region, Antibodies, Anti-Idiotypic, Rats, biology.protein, Female, Immunization, Immunoglobulin Light Chains, Antibody, Immunoglobulin Heavy Chains, Immunologic Memory, Sequence Alignment
Abstract

Immunological memory is characterized by a quick and enhanced immune response after re-exposure to the same antigen. To explain the mechanism involved in generation and maintenance of immunological memory, we had earlier proposed a hypothesis involving the relay of memory by idiotypic and anti-idiotypic B cells. The peptidomimic present in the hypervariable region of anti-idiotypic antibody was hypothesized to carry forward immunological memory. In the present work, we provide evidence supporting a role for the anti-idiotypic antibody in eliciting antigen-specific B-cell and T-cell responses. Employing the idiotypic monoclonal antibody $(Ab_1)$ specific for haemagglutinin (H) protein of rinderpest virus, $Ab_{2\beta}$ was generated, which possesses an internal image of the H protein in the region between amino acids 527 and 556. We demonstrate that antigen-specific memory is perpetuated by immunization with $Ab_2$, as shown by maintenance of antigen-specific T-cell responses upon restimulation in vitro of $Ab_2$ immune splenocytes by antigen-presenting cells expressing H protein or pulsed with H-protein-derived peptides. We have also shown that boosting with antigen-specific anti-idiotypic B cells generates a memory response in antigen-primed mice. Evidence has been provided for the existence of an antigen-specific B-cell idiotypic network in the body that supports the perpetuation of immunological memory as proposed in the relay hypothesis.

Published
2007

43. A CD8(+) T cell clone specific for antigen also recognizes peptidomimics present in anti-idiotypic antibody: Implications for T cell memory

Author

R. Nayak, Janakiraman Vani, and Melukote S. Shaila

Subjects
T cell, Immunology, Antigen presentation, Naive B cell, Immunoglobulin Variable Region, Epitopes, T-Lymphocyte, Hemagglutinins, Viral, Mice, Antigen, medicine, Cytotoxic T cell, Animals, Antigen-presenting cell, Antigens, Viral, Microbiology & Cell Biology, Antigen Presentation, Mice, Inbred BALB C, CD40, biology, Molecular Mimicry, Rinderpest virus, Antibodies, Anti-Idiotypic, Clone Cells, B-1 cell, medicine.anatomical_structure, biology.protein, Immunization, Peptides, Immunologic Memory, T-Lymphocytes, Cytotoxic
Abstract

The relay hypothesis [R. Nayak, S. Mitra-Kaushik, M.S. Shaila, Perpetuation of immunological memory: a relay hypothesis, Immunology 102 (2001) 387-395] was earlier proposed to explain perpetuation of immunological memory without requiring long lived memory cells or persisting antigen. This hypothesis envisaged cycles of interaction and proliferation of complementary idiotypic B cells (Burnet cells) and anti-idiotypic B cells (Jerne cells) as the primary reason for perpetuation of immunological memory. The presence of pepti-domimics of antigen in anti-idiotypic antibody and their presentation to antigen specific T cells was postulated to be primary reason for perpetuation of T cell memory. Using a viral hemagglutinin as a model, in this work, we demonstrate the presence of peptidomimics in the variable region of ail anti-idiotypic antibody capable of functionally mimicking the antigen derived peptides. A CD8(+) CTL clone was generated against the hemagglutinin protein which specifically responds to either peptidomimic synthesizing cells or peptidomimic pulsed antigen presenting cells. Thus, it appears reasonable that a population of activated antigen specific T cells is maintained in the body by presentation of peptidomimic through Jerne cells and other antigen presenting cells long after immunization. (C) 2007 Elsevier Inc. All rights reserved.

Published
2007

44. Peptidomimics of antigen are present in variable region of heavy and light chains of anti-idiotypic antibody and function as surrogate antigen for perpetuation of immunological memory

Author

Melkote S. Shaila, Janakiraman Vani, R. Chandra Nagasuma, R. Nayak, and Jayakani Justin

Subjects
Immunology, Molecular Sequence Data, Immunoglobulin Variable Region, Biology, Epitope, Mice, Antigen, BioInformatics Centre, Cell Line, Tumor, MHC class I, Animals, Amino Acid Sequence, Antigens, Antigen-presenting cell, Molecular Biology, Microbiology & Cell Biology, Mice, Inbred BALB C, Antigen processing, Molecular Mimicry, Virology, Antibodies, Anti-Idiotypic, Epitope mapping, Polyclonal B cell response, biology.protein, Female, Immunoglobulin Light Chains, Antibody, Immunoglobulin Heavy Chains, Peptides, Immunologic Memory, Epitope Mapping
Abstract

Peptide antigens composed of relevant B cell and T cell epitopes, capable of inducing protective immune response against the whole pathogen, are potentially safe, alternative vaccine antigens for prevention of wide range of diseases. Here, we show that short peptides derived from internal image sequences of anti-idiotypic antibody (peptidomimics) can function as both B and T cell epitopes and perpetuate antigen specific immunological memory.We have sequenced the variable regions of heavy and light chains of the anti-idiotypic antibody specific to rinderpest virus hemagglutinin protein and predicted T cell epitopes in these sequences by an immuno-informatics approach. We have studied the interaction of these epitopes with MHC class I by in vitro assays and in silico analysis by molecular modeling of the idiopeptide–MHC complexes as well as antigen-derived peptide–MHC complexes. The functional capacity of anti-idiotypic antibody derived peptides to stimulate antigen specific T cells in vitro was tested. The ability of peptidomimics to proliferate the immune splenocytes in vitro was 10 times more when compared with that of a control peptide taken from the constant region of immunoglobulin. Similarly three- to fivefold more amounts of IL-2 and IFN-gamma were secreted by immune splenocytes in response to in vitro re-stimulation with peptidomimics. Further, we have provided evidence for the generation of antibodies against peptidomimics in memory response generated on antigen or anti-idiotypic antibody immunizations. In summary, our experiments suggest that peptidomimics are generated in the body after antigen immunization and may have important roles in vivo in regulating antigen specific immunological memory.

Published
2006

45. A combined immuno-informatics and structure-based modeling approach for prediction of T cell epitopes of secretory proteins of Mycobacterium tuberculosis

Author

Nagasuma Chandra, Melkote S. Shaila, R. Nayak, and Janakiraman Vani

Subjects
Models, Molecular, Antigens, Bacterial, Protein Conformation, Protein subunit, In silico, Immunology, Computational Biology, Epitopes, T-Lymphocyte, Human leukocyte antigen, Plasma protein binding, Computational biology, Mycobacterium tuberculosis, Biology, Microbiology, Virology, Epitope, Infectious Diseases, Secretory protein, Antigen, Computer Simulation, Amino Acid Sequence, Peptide sequence, Protein Binding
Abstract

The role of secretory proteins of Mycobacterium tuberculosis in pathogenesis and stimulation of specific host responses is well documented. They are also shown to activate different cell types, which subsequently present mycobacterial antigens to T cells. Therefore identification of T cell epitopes from this set of proteins may serve to define candidate antigens with vaccine potential. Fifty-two secretory proteins of M. tuberculosis H37Rv were analyzed computationally for the presence of HLA class I binding nonameric peptides. All possible overlapping nonameric peptide sequences from 52 secretory proteins were generated in silico and analyzed for their ability to bind to 33 alleles belonging to A, B and C loci of HLA class I. Fifteen percent of generated peptides are predicted to bind to HLA with halftime of dissociation T(1/2) >or=100 min and 73% of the peptides predicted to bind are mono-allelic in their binding. The structural basis for recognition of no-namers by different HLA molecules was studied employing structural modeling of HLA class I-peptide complexes and there exists a good correlation between structural analysis and binding prediction. Pathogen peptides that could behave as self- or partially self-peptides in the host were eliminated using a comparative study with the human proteome, thus reducing the number of peptides for analysis. The implications of the finding for vaccine development are discussed vis-a-vis the limitations of the use of subunit vaccine and DNA vaccine.

Published
2005

Catalog

Books, media, physical & digital resources
See catalog results