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4 results on '"Janeiro, A.M."'

1. Quantitative Genetics Validates Previous Genetic Variants and Identifies Novel Genetic Players Influencing Alzheimer's Disease Cerebrospinal Fluid Biomarkers

Author

Matos, Mafalda Ramos de, Ferreira, C., Herukka, S.K., Soininen, H., Janeiro, A.M., Santana, I., Verbeek, M.M., Mendonca, A. de, Martins, M., Meulenbroek, O.V., Matos, Mafalda Ramos de, Ferreira, C., Herukka, S.K., Soininen, H., Janeiro, A.M., Santana, I., Verbeek, M.M., Mendonca, A. de, Martins, M., and Meulenbroek, O.V.

Abstract

Item does not contain fulltext

Published
2018

2. Quantitative Genetics Validates Previous Genetic Variants and Identifies Novel Genetic Players Influencing Alzheimer's Disease Cerebrospinal Fluid Biomarkers

Author

Matos, Mafalda Ramos de, Ferreira, C., Herukka, S.K., Soininen, H., Janeiro, A.M., Santana, I., Verbeek, M.M., Mendonca, A. de, Martins, M., Meulenbroek, O.V., Matos, Mafalda Ramos de, Ferreira, C., Herukka, S.K., Soininen, H., Janeiro, A.M., Santana, I., Verbeek, M.M., Mendonca, A. de, Martins, M., and Meulenbroek, O.V.

Abstract

Item does not contain fulltext

Published
2018

3. Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study

Author

Kruse, N., Persson, S., Alcolea, D., Bahl, J.M., Baldeiras, I., Capello, E., Chiasserini, D., Bocchio Chiavetto, L., Emersic, A., Engelborghs, S., Eren, E., Fladby, T., Frisoni, G., Garcia-Ayllon, M.S., Genc, S., Gkatzima, O., Heegaard, N.H.H., Janeiro, A.M., Kovacech, B., Kuiperij, H.B., Leitao, M.J., Lleo, A., Martins, M., Matos, M., Mollergard, H.M., Nobili, F., Ohrfelt, A., Parnetti, L., Oliveira, C.R., Rot, U., Saez-Valero, J., Struyfs, H., Tanassi, J.T., Taylor, P., Tsolaki, M., Vanmechelen, E., Verbeek, M.M., Zilka, N., Blennow, K., Zetterberg, H., Mollenhauer, B., Kruse, N., Persson, S., Alcolea, D., Bahl, J.M., Baldeiras, I., Capello, E., Chiasserini, D., Bocchio Chiavetto, L., Emersic, A., Engelborghs, S., Eren, E., Fladby, T., Frisoni, G., Garcia-Ayllon, M.S., Genc, S., Gkatzima, O., Heegaard, N.H.H., Janeiro, A.M., Kovacech, B., Kuiperij, H.B., Leitao, M.J., Lleo, A., Martins, M., Matos, M., Mollergard, H.M., Nobili, F., Ohrfelt, A., Parnetti, L., Oliveira, C.R., Rot, U., Saez-Valero, J., Struyfs, H., Tanassi, J.T., Taylor, P., Tsolaki, M., Vanmechelen, E., Verbeek, M.M., Zilka, N., Blennow, K., Zetterberg, H., and Mollenhauer, B.

Abstract

Item does not contain fulltext, Decreased levels of alpha-synuclein (aSyn) in cerebrospinal fluid (CSF) in Parkinson's disease and related synucleinopathies have been reported, however, not consistently in all cross-sectional studies. To test the performance of one recently released human-specific enzyme-linked immunosorbent assay (ELISA) for the quantification of aSyn in CSF, we carried out a round robin trial with 18 participating laboratories trained in CSF ELISA analyses within the BIOMARKAPD project in the EU Joint Program - Neurodegenerative Disease Research. CSF samples (homogeneous aliquots from pools) and ELISA kits (one lot) were provided centrally and data reported back to one laboratory for data analysis. Our study showed that although factors such as preanalytical sample handling and lot-to-lot variability were minimized by our study design, we identified high variation in absolute values of CSF aSyn even when the same samples and same lots of assays were applied. We further demonstrate that although absolute concentrations differ between laboratories the quantitative results are comparable. With further standardization this assay may become an attractive tool for comparing aSyn measurements in diverse settings. Recommendations for further validation experiments and improvement of the interlaboratory results obtained are given.

Published
2015

4. Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study

Author

Kruse, N., Persson, S., Alcolea, D., Bahl, J.M., Baldeiras, I., Capello, E., Chiasserini, D., Bocchio Chiavetto, L., Emersic, A., Engelborghs, S., Eren, E., Fladby, T., Frisoni, G., Garcia-Ayllon, M.S., Genc, S., Gkatzima, O., Heegaard, N.H.H., Janeiro, A.M., Kovacech, B., Kuiperij, H.B., Leitao, M.J., Lleo, A., Martins, M., Matos, M., Mollergard, H.M., Nobili, F., Ohrfelt, A., Parnetti, L., Oliveira, C.R., Rot, U., Saez-Valero, J., Struyfs, H., Tanassi, J.T., Taylor, P., Tsolaki, M., Vanmechelen, E., Verbeek, M.M., Zilka, N., Blennow, K., Zetterberg, H., Mollenhauer, B., Kruse, N., Persson, S., Alcolea, D., Bahl, J.M., Baldeiras, I., Capello, E., Chiasserini, D., Bocchio Chiavetto, L., Emersic, A., Engelborghs, S., Eren, E., Fladby, T., Frisoni, G., Garcia-Ayllon, M.S., Genc, S., Gkatzima, O., Heegaard, N.H.H., Janeiro, A.M., Kovacech, B., Kuiperij, H.B., Leitao, M.J., Lleo, A., Martins, M., Matos, M., Mollergard, H.M., Nobili, F., Ohrfelt, A., Parnetti, L., Oliveira, C.R., Rot, U., Saez-Valero, J., Struyfs, H., Tanassi, J.T., Taylor, P., Tsolaki, M., Vanmechelen, E., Verbeek, M.M., Zilka, N., Blennow, K., Zetterberg, H., and Mollenhauer, B.

Abstract

Item does not contain fulltext, Decreased levels of alpha-synuclein (aSyn) in cerebrospinal fluid (CSF) in Parkinson's disease and related synucleinopathies have been reported, however, not consistently in all cross-sectional studies. To test the performance of one recently released human-specific enzyme-linked immunosorbent assay (ELISA) for the quantification of aSyn in CSF, we carried out a round robin trial with 18 participating laboratories trained in CSF ELISA analyses within the BIOMARKAPD project in the EU Joint Program - Neurodegenerative Disease Research. CSF samples (homogeneous aliquots from pools) and ELISA kits (one lot) were provided centrally and data reported back to one laboratory for data analysis. Our study showed that although factors such as preanalytical sample handling and lot-to-lot variability were minimized by our study design, we identified high variation in absolute values of CSF aSyn even when the same samples and same lots of assays were applied. We further demonstrate that although absolute concentrations differ between laboratories the quantitative results are comparable. With further standardization this assay may become an attractive tool for comparing aSyn measurements in diverse settings. Recommendations for further validation experiments and improvement of the interlaboratory results obtained are given.

Published
2015

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