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CD4+ T cells play major roles in the adaptive immune system, which requires antigen recognition, costimulation, and cytokines for its elaborate orchestration. Recent studies have provided new insight into the importance of the supramolecular activation cluster (SMAC), which comprises concentric circles and is involved in the amplification of CD4+ T cell activation. However, the underlying mechanism of SMAC formation remains poorly understood. Here, we performed single-cell RNA sequencing of CD4+ T cells left unstimulated and stimulated with anti-CD3 and anti-CD28 antibodies to identify novel proteins involved in their regulation. We found that intraflagellar transport 20 (IFT20), previously known as cilia-forming protein, was upregulated in antibody-stimulated CD4+ T cells compared to unstimulated CD4+ T cells. We also found that IFT20 interacted with tumor susceptibility gene 101 (TSG101), a protein that endocytoses ubiquitinated T-cell receptors. The interaction between IFT20 and TSG101 promoted SMAC formation, which led to amplification of AKT-mTOR signaling. However, IFT20-deficient CD4+ T cells showed SMAC malformation, resulting in reduced CD4+ T cell proliferation, aerobic glycolysis, and cellular respiration. Finally, mice with T-cell-specific IFT20 deficiency exhibited reduced allergen-induced airway inflammation. Thus, our data suggest that the IFT20-TSG101 axis regulates AKT-mTOR signaling via SMAC formation.

Thyristor Controlled Reactor (TCR) has been used to improve power quality through firing angle control by parallel connection to the system in the form of ∆-connection. The inductances of three-phase TCR are assumed to be a same value, but a difference is generated in the inductance value of each phase in the manufacturing process. Due to the difference in three-phase inductance, a conventional TCR control algorithm causes the imbalance of phase voltage. So, this paper proposes a method to solve the phase imbalance problem of TCR using a modified firing angle control scheme, and the performance of the proposed method was verified through Matlab simulation.

T cells of the γδ lineage are unconventional T cells with functions not restricted to MHC-mediated antigen presentation. Because of their broad antigen specificity and NK-like cytotoxicity, γδ T-cell importance in tumor immunology has been emphasized. However, some γδ T-cell subsets, especially those expressing IL-17, are immunosuppressive or tumor-promoting cells. Their cytokine profile and cytotoxicity are seemingly determined by cross-talk with microenvironment components, not by the γδTCR chain. Furthermore, much about the TCR antigen of γδ T cells remains unknown compared with the extreme diversity of their TCR chain pairs. Thus, the investigation and application of γδ T cells have been relatively difficult. Nevertheless, γδ T cells remain attractive targets for antitumor therapy because of their independence from MHC molecules. Because tumor cells have the ability to evade the immune system through MHC shedding, heterogeneous antigens, and low antigen spreading, MHC-independent γδ T cells represent good alternative targets for immunotherapy. Therefore, many approaches to using γδ T cells for antitumor therapy have been attempted, including induction of endogenous γδ T cell activation, adoptive transfer of expanded cells ex vivo, and utilization of chimeric antigen receptor (CAR)-T cells. Here, we discuss the function of γδ T cells in tumor immunology and their application to cancer therapy., Cancer: T cell subset could boost immunotherapy An under-researched subset of T cells may find application in cancer immunotherapy once their multiple roles are fully understood. Jang Hyun Park and Heung Kyu Lee at the Korea Advanced Institute of Science and Technology, Daejeon, South Korea, have reviewed the current understanding of gamma delta (γδ) T cells, which are found in peripheral blood and specific tissues, for example, the skin. They form part of the immune response, but instead of recognizing antigens presented by MHC molecule as do other T cells, they respond to markers of cellular stress during events such as tumorigenesis. Evidence suggests γδ T cells play different roles in the tumor microenvironment depending on the proteins they produce and can induce anti-tumor or pro-tumor activity. The authors suggest that harnessing the anti-tumor properties of γδ T cells for use in immunotherapy could transform cancer treatments.

The anatomic location and immunologic characteristics of brain tumors result in strong lymphocyte suppression. Consequently, conventional immunotherapies targeting CD8 T cells are ineffective against brain tumors. Tumor cells escape immunosurveillance by various mechanisms and tumor cell metabolism can affect the metabolic states and functions of tumor-infiltrating lymphocytes. Here, we discovered that brain tumor cells had a particularly high demand for oxygen, which affected γδ T cell-mediated antitumor immune responses but not those of conventional T cells. Specifically, tumor hypoxia activated the γδ T cell protein kinase A pathway at a transcriptional level, resulting in repression of the activatory receptor NKG2D. Alleviating tumor hypoxia reinvigorated NKG2D expression and the antitumor function of γδ T cells. These results reveal a hypoxia-mediated mechanism through which brain tumors and γδ T cells interact and emphasize the importance of γδ T cells for antitumor immunity against brain tumors. Glioblastoma is one of the most intractable tumors and presents a hypoxic and immunologically cold microenvironment. Lee and colleagues demonstrate that normalizing oxygen tension unleashes γδ T cell anti-glioblastoma function.

A novel approximation-free differentiator-based output-feedback controller for uncertain large-scale systems (LSSs) is proposed. The considered LSS has nonautonomous and nonaffine-in-the-control subsystems which is yet to be tackled for decentralized output-feedback controller in the previous researches. The controller adopts a higher-order switching differentiator that can track the time-derivatives of a time-varying signal asymptotically. Through the differentiators, time-derivatives of output tracking errors are estimated and unstructured uncertainties in the controlled subsystems are compensated. The proposed decentralized output-feedback control formulae and the stability analysis are relatively simple in comparison to the previously proposed decentralized controllers. In this case, approximators such as fuzzy systems or neural networks are not required. The proposed controller guarantees that the tracking errors of the subsystems are asymptotically convergent to zeros and all the signals involved in the closed-loop systems are bounded.

Obesity is detrimental to the immune system. It impairs lymphatics, T cell development, and lymphopoiesis; induces dysfunction of antitumor immunity; and also promotes tumor progression. However, direct evidence of the impact of obesity on viral infection is lacking. We report a protective role of obesity against herpes simplex virus 2 infection of the genital mucosa in mice. Although conventional antiviral immunity is comparable between obese mice and lean mice, obesity enhances the cytotoxic subset of γδ T cells. This effect is mediated by L-arginine produced by commensal microbiota in the genital mucosa, which induces "pseudonormoxia" of γδ T cells, resulting in increased natural killer (NK) group 2 D (NKG2D) expression of γδ T cells through the downregulation of hypoxia-inducible factor 1-alpha (HIF1A) by inducing nitric oxide (NO) production, thereby protecting mice from lethal genital herpes. Thus, our work illuminates one mechanism by which obesity-induced compositional changes in the vaginal microbiota can affect mucosal immune responses against viral infection.

The Korean Institute of Technology Satellite (KITSAT-1) is the first satellite developed by the Satellite Technology Research Center and the University of Surrey. KITSAT-1 is orbiting the Earth’s orbit as space debris with a 1,320 km altitude after the planned mission. Due to its relatively small size and altitude, tracking the KITSAT-1 was a difficult task. In this research, we analyzed the tracking results of KITSAT-1 for one year using the Midland Space Radar (MSR) in Texas and the Poker Flat Incoherent Scatter Radar (PFISR) in Alaska operated by LeoLabs, Inc. The tracking results were analyzed on a weekly basis for MSR and PFISR. The observation was conducted by using both stations at an average frequency of 10 times per week. The overall corrected range measurements for MSR and PFISR by LeoLabs were under 50 m and 25 m, respectively. The ionospheric delay, the dominant error source, was confirmed with the International Reference of Ionosphere-16 model and Global Navigation Satellite System data. The weekly basis orbit determination results were compared with two-line element data. The comparison results were used to confirm the orbital consistency of the estimated orbits.

A novel output-feedback controller for uncertain nonautonomous nonlinear systems with unknown relative-degree is proposed in this study. With the assumption that the upper bound of the relative degree is known, the proposed control scheme is designed based on the input filter and higher-order switching differentiator(HOSD) with over dimension. Using the overestimated time-derivatives of tracking error by HOSD, the proposed controller compensates for the effects of uncertainties including an unknown relative degree in the controlled system. Assuming that the internal zero dynamics, if they exist, are stable, the asymptotic stability of the closed-loop system is guaranteed.

This paper proposed the transformer characteristic analysis method for the wireless power transfer (WPT) device and linear motor (LM) integration system that can be applied to industrial cleanroom transfer systems. A cable is required to supply the power in conventional systems. In comparison, the proposed system utilizes a WPT device that can simplify power transfers and make a better space utilization. The shape of the wireless power transmission system is proposed along with the discussion of the 2D FEA analysis method about the inductance analyzing method, which are important parameters in magnetic coupling. In addition, ferrite iron loss was calculated based on the analysis results, and applied to the entire modeling circuit to verify the validity of the measured and analyzed values. Finally, the proposed analysis method for the transformer coupling characteristics of the wireless power transfer combined with the transfer system is verified by experiments and simulations.

This paper presents a 10 kW, 12-slot 10-pole surface-mounted permanent magnet synchronous motor (SPMSM) design with fractional-slot concentrated winding for a podded propulsion system. Its load is a propeller that is proportional to the square of the rotational speed and the fifth power of the propeller diameter. Taking this into account, three SPMSMs with rated rotational speeds of 600, 1200, and 1800 rpm with the same rated output power of 10 kW were analyzed. These were designed under the same conditions (i.e., torque per rotor volume, air-gap length, current density, power factor, fill-factor, and supply voltage). Based on the SPMSMs designed by electromagnetic analysis, the housing of a podded propulsor for each SPMSM was modeled for mechanical analysis, including such parameters as forced vibration, radiated noise, and modal acoustics analysis in air and water. From the modal acoustics analysis, it is confirmed that the natural frequencies of a structure in water are lower than those in air because of the added mass effect of water.

The Optical Wide-field patroL-Network (OWL-Net) is a global optical network for Space Situational Awareness in Korea. The primary operational goal of the OWL-Net is to track Low Earth Orbit (LEO) satellites operated by Korea and to monitor the Geostationary Earth Orbit (GEO) region near the Korean peninsula. To obtain dense measurements on LEO tracking, the chopper system was adopted in the OWL-Net’s back-end system. Dozens of angle-only measurements can be obtained for a single shot with the observation mode for LEO tracking. In previous work, the reduction process of the LEO tracking data was presented, along with the mechanical specification of the back-end system of the OWL-Net. In this research, we describe an integrity assessment method of time-position matching and verification of results from real observations of LEO satellites. The change rate of the angle of each streak in the shot was checked to assess the results of the matching process. The time error due to the chopper rotation motion was corrected after re-matching of time and position. The corrected measurements were compared with the simulated observation data, which were taken from the Consolidated Prediction File from the International Laser Ranging Service. The comparison results are presented in the In-track and Cross-track frame.

A novel backstepping controller for uncertain single-input single-output pure-feedback nonaffine and nonautonomous nonlinear systems based on the time-derivative estimator (TDE) is proposed. Using TDEs, time-derivatives of error signals used in virtual control terms are directly estimated in every backstepping design steps. As a result, the control law has a relatively simple form. In addition, convergence of tracking error to a small neighborhood of origin is guaranteed regardless of unstructured uncertainties or unmatched disturbances in the controlled system. It does not require separate adaptive schemes or universal approximators such as neural networks or fuzzy logic systems adaptively tuned online to cope with system uncertainties. Simulation results demonstrated the simplicity and good performance of the proposed approximation-free controller.

Applying a concrete surface treatment method (epoxy or primer) can prevent water from penetrating concrete through surface pores. However, if the concrete surface is damaged, the subsequent reconstruction can be expensive and time-consuming. Concrete that is resistant to internal and external water has been extensively developed and used to supplement the surface treatment method. Herein, we prepared specimens by mixing cement mortar with fatty-acid-salt-based calcium stearate attached to two natural pozzolanic materials—diatomite and yellow clay. The physical tests measured (1) the air content, (2) flow test, (3) compressive strength, and (4) activity Factor. Durability experiments were performed on (1) the contact angle, (2) chloride ion diffusion coefficient, and (3) water absorption test. The results revealed that the compressive strength of concrete decreased as the calcium stearate content increased. Furthermore, it was confirmed that the contact angle of the test piece using the pozzolanic substance and calcium stearate was twice as high. It was confirmed that the sand test specimen had the highest water absorption rate, and the DT3% had the lowest. (Sand%: 11.8 > OPC: 6.5 > DT3%: 2.4), the chloride diffusion coefficient also showed similar results. (Sand%: 12.5 > OPC: 8.4 > DT1%: 8.8)Due to its unique insolubility, calcium stearate retards hydrate formation when mixed alone and negates compressive strength loss when combined with pozzolanic mixtures rich in SiO2 and Al2O3. Furthermore, the ideal method for producing water-resistant cement mortar is to evenly disperse calcium stearate in the porous powder of cement mortar.

Radar sensors are used for space situational awareness (SSA) to determine collision risk and detect re-entry of space objects. The capability of SSA radar system includes radar sensitivity such as the detectable radar cross-section as a function of range and tracking capability to indicate tracking time and measurement errors. The time duration of the target staying in a range cell is short; therefore, the signal-to-noise ratio cannot be improved through the pulse integration method used in pulse-Doppler signal processing. In this study, a method of improving the signal-to-noise ratio during range migration is presented. The improved detection performance from signal processing gains realized in this study can be used as a basis for comprehensively designing an SSA radar system.

Infiltrating tumor-associated macrophages (TAM) are known to impede immunotherapy against glioblastoma (GBM), however, TAMs are heterogeneous, and there are no clear markers to distinguish immunosuppressive and potentially immune-activating populations. Here we identify a subset of CD169

Permanent magnet Vernier machine (PMVM) is a strong candidate for direct-drive applications in low-speed region because its power characteristic is higher than conventional permanent magnet machine (PMM). In this paper, the design of a dual-stator PMVM (DSPMVM) with spoke-type rotor is introduced. As a radial motor with dual-stator configuration, one on the outer and inner side, the rotor is equipped with support bars for practical and simple manufacturing, which is the aim of this study. The characteristics and performance of the proposed machine with and without the support bar are examined with finite element analysis (FEA). The DSPMVM and the support were manufactured and tested through experiments to verify proposed structure. Both simulation and experiment results show that there is little to no difference in performance when the support bar is equipped. Furthermore, the average deviation between simulation and experiment results is approximately 7% which is within the acceptable range.

We aimed to analyze airborne microorganisms and assess air quality, temperature, and relative humidity at “J” Market, an arcade-type traditional market in Anseong (South Korea). Measurements were taken 16 times, twice per quarter (January, April, July, and October), at both the entrance and intersection of the market in 2020. The concentrations of airborne bacteria and fungi at the entrance and intersection were highest in October and lowest in April; however, they were below the recommended indoor levels (airborne bacteria

The detrimental role of obesity in various diseases, including metabolic, cardiovascular, autoimmune, infectious, and cancerous diseases, has been known for many years. Conversely, a phenomenon wherein obesity exerts a positive effect in certain conditions has recently been discovered, and this is known as the obesity paradox. However, the obesity paradox remains controversial, and the underlying mechanisms are still not clear. In particular, a possible role for obesity in response to viral infection has not been fully elucidated. In this study, we investigated a case of the obesity paradox in vaginal herpes simplex virus 2 (HSV-2) infection and uncovered the mechanisms whereby obesity protects from vaginal HSV-2 infection by inducing vaginal dysbiosis. Critically, these obesity-related vaginal microbiota produce L-arginine, which promotes γδ T cell immunity that enhances early protection against HSV-2 infection. This study reveals that obesity-induced translocation of gut microbiota to vagina potentiates anti-HSV-2 immunity via reprogramming of γδ T cell metabolism which provides novel insight for development of anti-HSV-2 drugs and understanding the role of microbiota, antiviral γδ T cell immunity, and mechanisms of obesity paradox. Supported by the National Research Foundation of Korea grant NRF-2019R1A2C208749013

Switched reluctance motor (SRM) is a low-cost, robust motor that is suitable for a wide range of applications. However, SRM has high torque ripple and acoustic noise, which is most apparent in the single-phase configurations. In this paper, the strategy and considerations in designing single-phase SRMs are presented. Permanent magnets (PMs) are included, making the structure hybrid. PMs help to secure the initial rotating position and act as another phase for continuous rotation. Finite element analysis (FEA) is performed to observe the characteristics of the models. Each proposed design is manufactured, and experiments were done to verify the effectiveness in practical application.

Respiratory syncytial virus (RSV) is a leading cause of respiratory tract infections in infants. Macroautophagy/autophagy is a catalytic metabolic process required for cellular homeostasis. Although intracellular metabolism is important for immune responses in dendritic cells, the link between autophagy and immunometabolism remains unknown. Here, we show that the autophagy-related protein ATG5 regulates immunometabolism. Atg5-deficient mouse dendritic cells showed increased CD8A+ T-cell response and increased secretion of proinflammatory cytokines upon RSV infection. Transcriptome analysis showed that Atg5 deficiency alters the expression of metabolism-related genes. Atg5-deficient dendritic cells also showed increased activation of glycolysis and the AKT-MTOR-RPS6KB1 pathway and decreased mitochondrial activity, all of which are cellular signatures for metabolic activation. These cells also showed elevated CD8A+ T-cell priming and surface major histocompatibility complex (MHC) class I expression. Our results suggested that ATG5 regulated host immune responses by modulating dendritic cell metabolism. These findings may help develop potential antiviral therapies that alter host immunity by regulating autophagy and immunometabolism. Abbreviations : 2-DG: 2-deoxyglucose; AAK1: AP2 associated kinase 1; AKT: AKT serine/threonine kinase; AM: alveolar macrophage; ATG: autophagy; ATP: adenosine triphosphate; BAL: bronchoalveolar lavage; BMDC: bone marrow dendritic cell; CSF2/GM-CSF: colony-stimulating factor 2 (granulocyte-macrophage); CTL: cytotoxic T lymphocyte; ELISA: enzyme-linked immunosorbent assay; GFP: green fluorescent protein; GSEA: gene-set enrichment analysis; H-2Db: H-2 class I histocompatibility antigen, D-B alpha chain; H-2Kb: MHC class I H2-K-b; HIF1A: hypoxia-inducible factor 1 alpha; IFNG: interferon-gamma; IL: interleukin; ITGAX: integrin alpha X; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MHC: major histocompatibility complex; MTORC1: mammalian target of rapamycin kinase complex 1; PBS: phosphate-buffered saline; PFU: plaque-forming unit; RLR: retinoic acid-inducible-I-like receptor; ROS: reactive oxygen species; RPMI: Roswell Park Memorial Institute; RPS6KB1/S6K: ribosomal protein S6 kinase, polypeptide 1; RSV: respiratory syncytial virus; Th: T helper; TLR: toll-like receptor; Treg: regulatory T cells; UMAP: uniform manifold approximation and projection.

Recently, because of the increase in the number of connections to Distributed Generation (DG), the problem of lowering voltage stability in the distribution system has become an issue. Reactive power compensators, such as Static Synchronous Compensators (STATCOM), may be used to solve the problem of voltage stability degradation. However, because of the complexity of the distribution system, it is very difficult to select the installation location for STATCOM. Furthermore, when installed in the wrong location, economical efficiency and availability problems may occur. This paper proposes a Virtual STATCOM Configuration and Control method that would operate like a single STATCOM based on multiple DGs connected to the system. The proposed Virtual STATCOM has the merit of being economical by using existing facilities without adding new power facilities, and it solves the problem of the difficulty of selecting the installation location because of the complexity of the distribution system. In addition, while the conventional STATCOM uses an independent control method in consideration of the power quality of the access point, the Virtual STATCOM performs the Point of Common Coupling (PCC) power quality compensation using the integrated control of multiple DGs connected to the system. In the proposed method, the Virtual STATCOM integrated control algorithm is configured by adopting linear programming, and the compensation is performed while considering the distance between DG and PCC, the inverter’s rated capacity, and the power generation. The performance of the Virtual STATCOM power quality compensation was verified using MATLAB/SIMULINK and Real Time Simulator (OPAL-RT).

Hypoxia is a hallmark of glioblastoma multiforme (GBM), the most aggressive cancer of the central nervous system, and is associated with multiple aspects of tumor pathogenesis. For example, hypoxia induces resistance to conventional cancer therapies and inhibits antitumor immune responses. Thus, targeting hypoxia is an attractive strategy for GBM therapy. However, traditional studies on hypoxia have largely excluded the immune system. Recently, the critical role of the immune system in the defense against multiple tumors has become apparent, leading to the development of effective immunotherapies targeting numerous cancer types. Critically, however, GBM is classified as a “cold tumor” due to poor immune responses. Thus, to improve GBM responsiveness against immunotherapies, an improved understanding of both immune function in GBM and the role of hypoxia in mediating immune responses within the GBM microenvironment is needed. In this review, we discuss the role of hypoxia in GBM from a clinical, pathological, and immunological perspective.

지속적인 우주개발은 인공위성의 지구 추락, 우주잔해물과 우주선 간의 충돌 등 우주위험의 발생 가능성을 크게 증가 시킨다. 국내에서는 이러한 우주위험을 감시하기 위한 광학감시체계 구축은 진행하였으나, 독자적인 상시 우주감시 정보 획득 능력을 갖는 우주감시 레이다기술에 대해서는 확보가 필요한 실정이다. 본 논...