Search

Made available in DSpace on 2022-05-01T06:02:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The oral lymphoepithelial cyst (OLC) is an uncommon lesion whose pathogenesis remains poorly understood. The aim of this study was to report the clinicopathologic features of the OLCs and to verify a possible association between OLCs and subgemmal neurogenous plaque (SNP) in the posterior lateral region of the tongue. A retrospective descriptive cross-sectional study was carried out. A total of 106,282 biopsy records of oral and maxillofacial lesions from six oral pathology services in Brazil were analyzed. All cases of OLCs were reviewed, and clinical and histopathological data were collected. Immunohistochemical reactions for S-100 protein were performed to confirm the diagnosis of SNP. Among all lesions, there were 132 (0.11%) cases of OLCs. The series comprised 83 females (62.9%) and 49 males (37.1%), with a 1.7:1 female-to-male ratio and a mean age of 45.8 ± 17.7 years. Most cases involved the tongue (n = 80; 62.0%) and presented clinically as asymptomatic papules or nodules with a yellow or whitish color. Microscopically, most of the cysts were entirely lined by parakeratinized stratified epithelium (n = 89; 67.4%) and filled with desquamated cells, keratin debris, amorphous eosinophilic material, and inflammatory cells in varying amounts. Connection with the epithelium of oral mucosa was observed in 18 cases (13.6%). SNP was found in 9/80 (11.2%) cases involving the tongue. The clinical and demographic features of OLCs were similar to those described in previous studies. Overall, this lesion has a predilection for the posterior region of the tongue of female adults. Clinicians must include the OLC in the differential diagnosis of yellow/white papules and nodules of the oral cavity. Department of Oral Diagnosis Piracicaba Dental School University of Campinas (UNICAMP) Department of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP) School of Dentistry University of Fortaleza (UNIFOR) Department of Oral Diagnosis and Pathology School of Dentistry Federal University of Rio de Janeiro (UFRJ) School of Dentistry State University of Rio de Janeiro (UERJ) Oral Pathology Section Department of Oral Diagnosis Piracicaba Dental School University of Campinas (UNICAMP), Av. Limeira 901, P.O. Box 52 Department of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)

Submitted by Jaqueline Lemes Ribeiro (jaqueline.ribeiro@unesp.br) on 2021-08-16T20:37:27Z No. of bitstreams: 1 Manuscript_R1.pdf: 700112 bytes, checksum: f03bdcfd7dccfcc1cd700da04e819d3d (MD5) Approved for entry into archive by Silvana Alvarez null (silvana@ict.unesp.br) on 2021-08-18T17:02:02Z (GMT) No. of bitstreams: 1 ribeiro_jl_preprint_sjc_oral.pdf: 700112 bytes, checksum: f03bdcfd7dccfcc1cd700da04e819d3d (MD5) Made available in DSpace on 2021-08-18T17:02:02Z (GMT). No. of bitstreams: 1 ribeiro_jl_preprint_sjc_oral.pdf: 700112 bytes, checksum: f03bdcfd7dccfcc1cd700da04e819d3d (MD5) Previous issue date: 2021-07 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background: Pyogenic granuloma (PG) is a lesion characterized by the proliferation of blood vessels, commonly affecting the skin and the mouth. We aimed to compare clinical, microscopic, and immunohistochemical features of the two types of oral PG: lobular capillary hemangioma (LCH) and non-LCH (NLCH). Methods: Epidemiological and clinical data from 2000 to 2018 were collected from the archives of our institution, and histopathological sections of PG were reviewed. Immunohistochemical analyses (CD34, D2-40, SMA, mast cell, and Ki-67) were performed in 34 cases. Results: Sixty-two LCH and 107 non-LCH samples were included. The mean (±SD) age of the patients was 38.59 ± 16.96 years; 55.62% were female; 39.64% of cases occurred in the gingiva, 44% of the nodules were pedunculated, and 13.02% of patients reported a history of trauma. NLCH was more prevalent among older patients than LCH. The most prevalent site of LCH was the lips, while NLCH occurred more in the gingiva (P < 0.05). Epithelial atrophy, microvessels, SMApositive areas, and Ki-67-positive nuclei were more prevalent in LCH (P < 0.05). Conclusions: PG accounted for 2.25% of lesions archived in the pathology service and most cases were NLCH. LCH and NLCH exhibited clinicopathological differences in terms of age, site, epithelial atrophy, vascularization, and proliferation rate. Preprint Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil.

Submitted by Luana Priscila Costa (luana@reitoria.unesp.br) on 2020-09-28T19:37:32Z No. of bitstreams: 1 moraes_rm_preprint_sjc_live.pdf: 719766 bytes, checksum: a9e3b89c126b7e394531c62ad32351b9 (MD5) Approved for entry into archive by Luana Priscila Costa (luana@reitoria.unesp.br) on 2020-09-28T19:43:17Z (GMT) No. of bitstreams: 1 moraes_rm_preprint_sjc_live.pdf: 719766 bytes, checksum: a9e3b89c126b7e394531c62ad32351b9 (MD5) Made available in DSpace on 2020-09-28T19:43:17Z (GMT). No. of bitstreams: 1 moraes_rm_preprint_sjc_live.pdf: 719766 bytes, checksum: a9e3b89c126b7e394531c62ad32351b9 (MD5) Previous issue date: 2020-09-08 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective Lactobacillus reuteri is reported as a beneficial adjunct to conventional periodontitis treatment or as monotherapy for gingivitis in several clinical trials. However, probiotics are not free from the risk of adverse effects. We investigated the effects of live and heat-killed (paraprobiotic) L. reuteri during the development of ligature-induced periodontitis in rats. Methods Thirty-two male rats were divided into four groups—control group: healthy rats, LIP group: rats with ligature induced periodontitis, LIP/PRO group: rats with periodontitis that received live L. reuteri, LIP/PARA group: rats with periodontitis that received heat-killed L. reuteri. Treatment was administered for 30 days prior to ligation and continued for 14 days until euthanasia. The hemimandibles were removed and submitted to radiographic analysis, microcomputerized microtomography (μCT) and histomorphometric analysis. Results Radiographic analysis revealed that pro- and paraprobiotics enhanced the percentage of mesial bone support. The μCT analysis showed that paraprobiotic treatment increased bone volume and trabecular number while decreasing total bone porosity and trabecular separation. Live probiotic led to higher trabecular thickness. In the histomorphometric analysis, paraprobiotics significantly prevented furcation bone loss and collagen breakdown, although both pro- and paraprobiotics similarly reduced mesial bone loss. There were no significant differences in attachment loss or the number of tartrate-resistant acid phosphatase -positive cells across treatments. Conclusions Although viable and heat-killed bacteria reduced mesial alveolar bone loss and ameliorated furcation microarchitecture parameters, paraprobiotics presented the most pronounced results preventing periodontitis development. This can contribute to the future use of paraprobiotics in the prevention and treatment of periodontitis. Preprint Universidade Estadual Paulista Julio de Mesquita Filho - Unesp FAPESP: 2016/06946-1 FAPESP: 2017/26461-5

Vascular leiomyomas (VL) are benign lesions of perivascular origin. We report two new cases and discuss their clinical, histological and immunohistochemical characteristics, in order to facilitate the diagnosis and treatment of such lesions. The patients, both male, presented asymptomatic nodules located in the bottom of the labial sulcus and buccal mucosa. In the second case, color doppler ultrasonography was performed, which showed no change in blood flow. After excisional biopsies, a limited lesion was observed histologically, with multiple tortuous vessels of varying sizes and calibers, and among them, spindle cells bundles, positive for smooth muscle actin. Oral VLs have clinical features similar to those of other more common lesions, making preoperative diagnosis difficult. Imaging examination, such as color doppler ultrasonography, may help in diagnosis. In general, excisional biopsy is performed, due to the ease of removal of the lesion during surgery. The treatment of choice is the complete excision of the lesion, which has an excellent prognosis and a low rate of recurrence. Key words:Vascular leiomyoma, spindle cells, thrombus, Doppler.

Subgemmal neurogenous plaque (SNP) is a neural structure present in the posterolateral border of the tongue, forming a morphophysiological complex with the taste buds. It was first described 20 years ago, and still now it is not well recognized by clinicians and pathologists. The aim of this study is to report 3 cases of SNP incidentally found after biopsies of posterior tongue lesions (lymphoepithelial cyst, lymphatic malformation, and mucoepidermoid carcinoma). The plexus usually exhibits a biphasic pattern, with bundle of fusiform wavy cells parallel to the epithelial surface, and a deepest portion composed of small nerve fascicles. The clinical findings of the SNP are unspecific, but several cases are symptomatic. The histologic pattern can be mistaken for soft tissue reactive or neoplastic neural lesions, which makes the recognition of the morphologic aspects of this anatomic structure very important.