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A Schiff base ligand, 3-((6-amino-1,6-dihydropyridin-2-yl)imino)indolin-2-one, was synthesized by condensing isatin with 2,6-diaminopyridine in this study. Elemental analysis, NMR spectroscopy, and infrared spectroscopy enabled confirmation of the structure of the pyridine incorporated ligand. Additional coordination compounds of this ligand with Mn(II), Co(II), Ni(II), Cu(II), Zn(II), and Cd(II) metals were synthesized with metal-to-ligand proportions verified through trace elemental examinations using CHN. Various spectroscopic techniques including UV-visible absorption spectrophotometry, IR spectrophotometry, electrical conductivity quantifications, melting point determinations, and magnetic susceptibility analyses facilitated characterization of the produced complexes. The antimicrobial potential of the organic ligand and inorganic complexes was screened against Bacillus subtilis and Staphylococcus aureus bacterial strains through the agar disc diffusion technique. Both the uncoordinated ligand and metal-ligand coordination compounds displayed appreciable antibacterial effects on the tested microorganisms. Tetrahedral configurations were proposed for all prepared complexes based on combined analytical and spectroscopic evidence. [ABSTRACT FROM AUTHOR]

A precise, reproducible, rapid, and highly sensitive spectrophotometric procedure was created for the micro determination of Tetracycline hydrochloride (TCH) in bulk form and pharmaceutical formulation. The suggested method depends on the proton transfer reaction by coupling the substance to be determined with the reagent 2,6-diaminopyridine in the basic medium to form a colored complex that shows the maximum absorption value at the wavelength of 385 nm. To reach the optimum conditions, different factors were studied. It was observed that Beer's law is followed in the range of (3-32 µg/mL) with a molar absorptivity of 18.755 L/mol.cm. The methods indicated a high degree of sensitivity in terms of limit of detection which was described to be as low as 0.137 µg/mL while the quantification limit was 0.458 µg/mL. The average recovery value was 97.45%, and the relative standard deviation value (RSD) was less than 4.3%. The correlation ratio with the detector was 2:1 (drug: reagent). Therefore, the proton transfer reaction was then applied successfully for the quantitative assay of tetracycline in its pharmaceutical preparations with satisfactory results. [ABSTRACT FROM AUTHOR]