Search

This research aimed to investigate the use of essential oils (EO) in vivo as a feed additive on rumen fermentation characteristics and methane gas through a meta-analysis approach by integrating various related studies. The data analyzed came from 21 journals, 26 studies, and 73 data points from 2007–2021, which were tabulated using Microsoft Excel and analyzed with SAS using the mixed model method. The results of EO supplementation exhibited a significant (P < 0.01) decrease in ammonia concentration, Diplodinium population, and methane gas production, as well as a significant (P < 0.05) reduction of acetate proportion and Dasytrica populations. In contrast, EO supplementation had a significant effect (P < 0.01) on final body weight increase. The crude protein tended to increase (P < 0.1) with EO supplementation. In conclusion, EO supplementation as a feed additive can positively affect rumen fermentability and methane gas production.

Mycotoxin-contaminated feed is one of the main causes of contamination that may increase broiler mortality and impair their production. A total of 161 in vivo data acquired from 31 published articles were used as a database for determining the effectiveness of mycotoxin binders on performance and health of broiler. The Hedges’ d value was employed as a measure of effect size (ES) in the present meta-analysis. Increasing mycotoxin binder in feed enhanced body weight (ES=0.784; P

Background and Aim: Rice straw, a widely available agricultural byproduct globally, has significant potential as a basal diet for livestock. The major challenge lies in obtaining high-protein foliage that can be easily extracted using natural water rather than chemical solvents. This study aimed to assess the ability of distilled water to extract protein concentrate from Indigofera leaves (Indigofera zollingeriana Miq.) and to evaluate its effectiveness in enhancing rumen feed fermentation and digestibility in low-quality rice straw basal diets. Materials and Methods: The study was conducted in two experimental series. Experiment 1 was designed to explore the ability of distilled water to extract protein concentrate from fresh and dry Indigofera leaves by comparing it with the 0.1 N NaOH standard solvent. Experiment 2 focused on the in vitro digestibility of protein concentrates extracted from fresh Indigofera leaves based on optimal findings from experiment 1. Five treatments consisting of 0.5% and 1.0% protein concentrate and two extractants (distilled water and 0.1 N NaOH) were used to extract protein from Indigofera leaves. These extracts were then added to rice straw-based diets. Rice straw without supplements was used as a control. The treatments were arranged using a randomized complete design with five replicates. Results: The results of experiment 1 showed that distilled water was superior to 0.1 N NaOH for extracting protein concentrate from fresh Indigofera leaves, as revealed by higher dry matter, protein yield, total amino acids (AA), and total essential AA (EAA) production. For in vitro experiment 2, supplementation with distilled water-extracted protein concentrates successfully increased rumen fermentation and digestibility in rice straw basal diets, as indicated by higher gas production, total volatile fatty acid, and microbial protein levels compared with 0.1 N NaOH. Conclusion: Findings from this study confirm that Indigofera leaf protein concentrate offers a new alternative for enhancing rumen feed fermentation and the digestibility of low-quality rice straw diets. This study implies that it is an easy, cost-effective, and environmentally friendly approach, particularly beneficial for smallholders, to extract protein concentrate from fresh Indigofera leaves using distilled water and use it to enhance the quality of rice straw for ruminant feed. The limitation of this study is that the Indigofera supplement was established using in vitro digestibility under controlled laboratory conditions, which does not reflect real rumen conditions. Therefore, further studies using in vivo digestibility in ruminant animals are required to confirm the ability of the protein extracted from indigofera to enhance rumen feed fermentation in low-protein basal diets.

Controlled/slow-release fertilizers (CSRF) continue to be developed because of the need to increase agricultural productivity and reduce the economic, social, and environmental impacts of conventional fertilizers leaching into nature. Additionally, the use of biodegradable polymers as CSRF materials continues to be a priority in CSRF development. Therefore, this review synthesizes the current research on lignin- and polysaccharide-based CSRFs and emphasize their compatibility and performance in agricultural applications. Lignin, an abundant and renewable biopolymer, was evaluated for its effectiveness as a slow-release agent in CSRF. Studies have demonstrated the potential of lignin to improve nutrient release profiles and environmental sustainability when used alone or in combination with other polymers. Polysaccharides, which are known for their biocompatibility and biodegradability, have also been explored. The incorporation of lignin into polysaccharide-based CSRFs has been highlighted, particularly in starch, cellulose, chitosan, and sodium alginate matrices. These composites offer improved mechanical properties, controlled nutrient release, and enhanced soil water retention. The challenges and future directions regarding lignin- and polysaccharide-based CSRF are also reviewed. These findings underscore the importance of developing sustainable fertilizer technologies to meet future food demands while mitigating environmental impacts.

Background and Aim: Zinc (Zn) is important for various physiological processes in broiler chickens, including protein and carbohydrate metabolism, growth, and reproduction. The gastrointestinal absorption of Zn in broiler chickens was notably low. One approach that has been explored for enhancing the bioavailability of Zn is the development of Zn nanoparticles (NPs). Zn is required for various physiological processes in broiler chickens, including protein and carbohydrate metabolism, growth, and reproduction. Therefore, this study aimed to assess the impact of conventional Zn and Zn NPs on broiler chickens using a meta-analysis methodology. Materials and Methods: A database was built from published literature to evaluate the effects of the addition of Zn NPs and conventional Zn on broiler chicken responses, including the following parameters: production performance; carcass cuts; visceral organ weight; lymphoid organ weight; nutrient digestibility; intestinal villi; mineral Zn, calcium, and phosphorus concentrations; hematology; blood parameters; immunoglobulin; and intestinal bacterial population. Various scientific platforms, including Scopus, Web of Science, PubMed Central, and Google Scholar, were used to search for peer-reviewed articles. A database was created from 25 studies that met the inclusion criteria. The data were then processed for a meta-analysis using a mixed-model methodology. Different types of Zn (NPs versus conventional) were considered fixed effects, different studies were treated as random effects, and p-values were used as model statistics. Results: Across the parameters observed in this study, the use of Zn NPs was more efficient in Zn utilization than conventional Zn, as evidenced by the average dose of Zn NPs being much lower than that of conventional Zn (79.44 vs. 242.76 mg/kg) yet providing similar (p > 0.05) or even significantly better effects (p < 0.05) compared to conventional Zn usage. Conclusion: This investigation revealed the beneficial influence of Zn NPs in broiler chickens compared to the conventional utilization of Zn through an all-encompassing meta-analysis. Moreover, Zn NPs have proven to be more effective in Zn utilization when juxtaposed with conventional Zn, as demonstrated by the significantly lower quantity of Zn NPs administered compared to conventional Zn, while yielding comparable or even superior outcomes compared to the traditional utilization of Zn. A limitation of this study is that the Zn NPs used were sourced from inorganic Zn NPs. Therefore, future research should focus on evaluating the efficiency of organic Zn NPs in broiler chicken feed.

Fatty acid content and carcass characteristics are two meat quality traits consumers choose to ob tain healthy and high-quality lamb. Genetic improvement of fatty acid content and carcass characteris tics in sheep is important to increase the public appeal of lamb meat. The Biglycan gene (BGN) is a gene that functions to regulate the growth and maintenance of connective tissues, such as bone and cartilage. This study examined the polymorphism of the BGN gene to get a deeper understanding of the correlation between fatty acid content and carcass traits in Indonesian sheep. The PCR-RFLP ap proach was used to detect polymorphism in the BGN gene in meat samples obtained from the longissi mus dorsi region of 115 rams aged between 10 and 12 months. The General Linear Model (GLM) was used test to analyze genotyping through association studies between genotypes and phenotypic attrib utes. The results indicated that the carcass' fatty acid composition, including tridecanoic acid (C13:0), arachidonic acid (C20:4n-6), and polyunsaturated fatty acids (PUFA), as well as the BGN gene with SNP point g. 77807325 G>C (GG and CC genotypes), were significantly (PC can be a potential genetic marker for selecting fatty acid (tridecanoic acid C13:0), pol yunsaturated fatty acid (PUFA), arachidonic acid), carcass percentage, and carcass length in sheep meat in Indonesia.

Background: The Trichuris eggs are collected from naturally infected sheep. Natural antihelmintics such as herbal medicines are needed as an alternative, such as natural compounds from endemic plants. Aim: This present study aims to evaluate the ovicidal activity and cytotoxicity effects of ethanolic extract of Curcuma longa (EECL) and Camelia sinensis (EECS) as a biological anthelmintic against the egg of Trichuris sp. Methods: The Trichuris eggs are collected from naturally infected sheep. CMC-Na solution 1% was used as control. The treatments were 0.12% EECL; 0.24% EECL; 0.15% EECS; 0.30% EECS; combination of 0.12% EECL and 0.30% EECS; combination 0.24% EECL; and 0.15% EECS. Ovicidal activity testing by microscopic examination of eggs treated using different concentrations of EECL extract, EECS and a combination of them. They were exposed for various times (7, 14, 21 and 28 days) and incubated at room temperature. Results: The study shows that combination of Curcuma longa extract and tea extract exhibits good ovicidal anthelmintic activity against the Trichuris sp. in sheep. Cytotoxicity examination using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) test. Based on MTT data processed using regression analysis, the obtained LC50 from administration of EECL, EECS and a combination of both in a ratio of 1:1, 2:2, 1:2 and 2:1. The combination of EECL extract and EECS with the highest concentration produced cell viability of 28.46%, 17.25%, 56.01%, and 46.47%, respectively. Conclusion: It can be concluded that the most cytotoxic ingredient is found in the combination of EECL and EECS (2:2) at 17.25% and the safest is in the ratio (1:2) at 56.01%. [Open Vet J 2024; 14(6.000): 1467-1475]

Blanching is the most common pretreatment method for fruits and vegetables to maintain product quality. Numerous studies revealed desirable impacts of the process to total phenolic content, while the others showed opposite results. In addition, some differences in the blanching condition such as methods and classification of fruits and vegetables were also known able to affect total phenolic content. This study aimed to analyze the effect of blanching on total phenolic content in vegetables and fruits through a meta-analysis approach. Study selection was conducted with a PRISMA method. There were 28 articles and 82 data used for meta-analysis. The data were analyzed by Hedges’d to calculate the effect size with confidence interval (CI) utilizing OpenMee software. The result showed that blanching demonstrated significant effects on the rise of total phenolic content (hedges’d[95% CI]: 0.782 [0.037 to 1.527]; p

The mechanical advances of this cutting-edge time have significantly affected the manner in which individuals live, the most significant of which is in the part of communication. Handheld phones are communications devices that are being used today. The large number of customers' demands for smart phones due to the advantages of the features they possess make companies compete to be far superior to their rivals. The examination is pointed toward taking a gander at the impact of brand picture and item quality on iPHONE buy choices in Denpasar City. The method used is a partial least square with three latent variables in particular brand image, product quality, and purchase decision. The results obtained are brand image & product quality effect on the iPHONE purchase decision in Denpasar city. Brand image and product quality can explain or affect iPHONE purchase decisions by percent.

Background and Aim: In the livestock sector, particularly ruminants, an approach to minimize methane emissions can be carried out through a feeding strategy involving herbal plants containing bioactive compounds that can reduce protozoa and decrease methane gas emissions. The aim of this in vitro study was to analyze the effects of herbal plant supplementation on rumen fermentation, total gas, and methane production, in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), and protozoa populations within the rumen. Materials and Methods: Two experiments were conducted in this study. Experiment 1 was conducted to determine the most promising herbal plants capable of increasing total gas production and reducing protozoan populations. Three potential herbals selected in Experiment 1 were continued in Experiment 2 as supplements in the palm kernel meal (PKM)-based ration (70% PKM + 30% herbal plants). Results: Experiment 1 revealed that Eurycoma longifolia (EL), Cola acuminata (CLA), and Cassia alata (CSA) were potential herbal candidates for enhancing total gas production and the percentages of IVDMD and IVOMD. In Experiment 2, supplementation with EL, CLA, and CSA significantly increased IVDMD from 62.84% to 70.15%, IVOMD from 61.61% to 53.18%, and NH3 from 13 mM to 17 mM, as well as reduced partial volatile fatty acids and total gas production. In addition, the methane gas and protozoan populations were reduced. Conclusion: The utilization of EL, CLA, and CSA effectively increased the production of total gas, IVDMD, and IVOMD while reducing methane gas protozoa populations in rumen fermentation compared with the control.

Objective: Different sources and levels of starch in the total mixed ration might result in different rumen fermentation profiles, rumen microbial composition, and enteric methane production. The current study aimed to evaluate the effect of dietary starch intake on enteric methane production through a meta-analytical approach by integrating related published studies. Materials and Methods: Papers that provided study results on enteric methane production from cattle fed different sources and levels of starch were selected. A total of 52 publications were filtered based on some specified criteria, comprised of 73 studies focused on enteric methane production in cattle fed a basal diet supplemented with starch. The collected data were subjected to statistical meta-analysis through a mixed methodology model. The p-value and root mean square error (RMSE) were applied as the statistical models. Results: Results showed that increasing the level of dietary starch intake and its digestibility in the rumen as well as increasing propionate significantly decreased the acetate to propionate (A/P)ratio as well as enteric methane production (p < 0.01). Conclusions: It is concluded that the addition of starch to diets mitigates enteric methane production from cattle, primarily through two ways, i.e., manipulation of the protozoa population and alteration to a lower A/P ratio. [J Adv Vet Anim Res 2024; 11(1.000): 212-230]

Background and Aim: Irradiated chitosan can be used as a matrix for slow-release urea (SRU) production. This study aimed to (1) determine the optimal formulation of irradiated chitosan matrix for controlling nitrogen release and (2) evaluate the characteristics of SRU in vitro fermentation based on irradiated chitosan as a feed supplement. Materials and Methods: In the first phase of the investigation, four chitosan-based SRU formulations with varying amounts of acrylamide (3 and 5 g) and gamma irradiation (5 and 10 kGy) were evaluated. Scanning electron microscopy, Fourier transform mid-infrared spectroscopy, and ammonia release characteristics were used to observe morphological, functional group, and ammonia release characteristics. In the second phase of research, the most effective SRU formulation was utilized as a supplement to ruminant rations based on rice straw, sorghum straw, and alfalfa. Gas production, rumen fermentation characteristics, and methane gas production were observed in vitro. Results: On the basis of surface image analysis, the four SRU formulas generate a similar appearance. Compared with untreated urea, the SRU3 formula reduced the percentage of ammonia emission by 12.85%–27.64% after 24 h of incubation (p = 0.05), as determined by the first phase study. SRU3 became the basis for the second testing phase. The addition of SRU3 did not affect the optimal gas production in vitro. SRU3 treatment produced less gas than Optigen® treatment (p = 0.05). With regard to rumen fermentation and digestibility, Optigen® yielded better results than SRU3 (p = 0.05). However, the treatment with SRU3 resulted in reduced methane production compared to that in the control (p = 0.05). Conclusion: Irradiated chitosan as an SRU matrix may control the release of ammonia in the rumen medium. The SRU3 formulation is the most effective. The addition of SRU to rice straw-based rations reduces methane production without affecting in vitro digestibility.

There are many factors affecting human life, one of which is climate. Differences in climatic conditions in each region result in differences in the environment in society. The differences are referred to as potential natural resources, livelihoods, and social cultural conditions. The climate has an impact on culture in terms of how people dress, the shape of houses, and so on. The regency group in Central Java Province is based on similarities in climate characteristics using fuzzy clustering. The data used were taken from Central Java Provincial Statistical Office in 2022.The results of district grouping in Central Java Province are based on similarities in climate characteristics using fuzzy clustering with 4 different number of clusters and the validity tests of the Partition Coefficient and Classification Entropy indices. Based on the results of the index validity test, the optimal grouping results are 2 clusters with a Partition Coefficient value of 0.911233 and a Classification Entropy value of 0.07979. The 1st cluster consists of 13 districts with a cluster center at 27.9°C for air temperature, 2418mm for rainfall, and 804% for keelThe 2nd cluster and airbase consist of 22 cluster central districts at 26.6 °C for air temperature, 4087 mm for rainfall, and 81.3% for humidity.

Background and Aim: Successful rearing of laying hens to achieve optimal egg production is an endeavor that often faces various constraints and challenges, such as infectious diseases, environmental stressors, and fluctuations in feed quality. The incorporation of essential oils (EOs) into the diet of laying hens has attracted considerable attention in recent years. Therefore, our study aimed to evaluate the efficacy of EO inclusion in laying hen diets by considering the effects of production phase and breed on performance, egg quality, serum biochemistry, gut health, and antioxidant activity. Materials and Methods: The articles were obtained from the Web of Science, Scopus, Science Direct, and PubMed using the search terms “essential oils,” “laying hens,” and “phytobiotics.” Data from 27 articles and 71 experiments were grouped according to laying hen production phase and breed in the database. The EO levels ranged from 0 to 1000 mg/kg, with thymol and carvacrol being the major EOs. A mixed model was used to analyze the data. Random effects were applied to the treatment, and fixed effects were applied to EO level, production phase, and breed. Results: Egg production, feed intake, feed efficiency, eggshell quality, villus height, crypt depth, superoxide dismutase, and glutathione peroxidase levels increased linearly (p = 0.05) and egg weight and mass increased quadratically (p < 0.05) with increasing EO concentrations. An interaction was observed between the EO level egg production and feed conversion ratio (p = 0.05). Serum glucose, cholesterol, and malondialdehyde levels decreased with increasing EO concentrations (p < 0.05). Conclusion: The inclusion of EOs effectively increased egg production, feed efficiency, egg weight, egg mass, eggshell quality, oxidative enzymes, and intestinal health. In addition, the proportion of dietary EOs in lightweight laying hens was higher than that in semi-heavy-weight laying hens in improving egg production and feeding efficiency.

Starch is extensively utilized in food processing for various purposes. However, the use of native starch is limited due to its unsuitability with processing conditions or products characteristics. Physical modification of starch is commonly employed to enhance the properties of native starch. The physical starch modification using microwave heating is presently developed due to its more efficient energy consumption than that of traditional heating methods. The process of microwave heating followed by cooling has been found to induce the formation of type 3 resistant starch (RS3). However, the effects of microwaving heating towards the the increase of resistant starch contents varies among researchers. For this reason, a meta-analysis was conducted to evaluate the effect of microwave heating on the levels of resistant starch in carbohydrate sources such as cereals, pulses, and tubers. The objective of this study was to analyze the carbohydrate food groups that demonstrated the most significant increase in resistant starch levels due to microwave heating, and to determine the optimal microwave treatment parameters within these food groups using meta-analysis. The findings indicate that microwave heating treatment is particularly effective for cereals, with rice being the most responsive. The most favorable treatment parameters include a power range of 401-600 W, heating time of 60-99 s, and starch moisture content of 40-60%.

A meta-analysis was conducted to determine the effects of different types of oligosaccharides (OS) on the egg quality, intestinal profile, nutrient digestibility, and immunity of laying hens. A dataset was constructed from articles published between 2006 and 2023 via a literature search using several keywords related to oligosaccharides and laying hens’ performance. After careful evaluation, the final dataset was developed from 25 in vivo studies comprising 165 comparisons. The meta-regression showed that levels of OS supplementation curvilinearly increased (p 0.05) feed intake (FI), feed conversion ratio (FCR), egg mass, egg weight, eggshell thickness, shell strength, and Haugh unit. OS supplementation showed a positively improved antioxidant activity, as indicated by the curvilinear effect on superoxide dismutase (SOD) and malondialdehyde (MDA) concentration. In the categorical meta-analysis, mannan oligosaccharide (MOS), xylooligosaccharides (XOS), and chitosan oligosaccharide (COS) significantly increased (p

The present research aimed to evaluate the effects of extruded feed ingredients on the growth performance and apparent ileal digestion of broilers using a meta-analysis. The database was developed based on 30 studies comprising 150 data points. The data were analysed using the OpenMEE software, considering the extrusion of feed ingredients as fixed effects and individual studies as random effects. Results showed that the extruded feed ingredients enhanced weight gain (p

A meta-analysis was conducted to determine the effects of probiotics on the performance, blood parameters, nutrient digestibility and carcase quality of domesticated rabbits. A dataset was constructed based on relevant published papers. An algorithm was constructed from 2004 to 2022, with a search in Scopus, Web of Science, Pub Med, and Medline using the MESH terms ‘probiotics’, ‘rabbit’, ‘performance’, ‘blood parameters’, ‘nutrient digestibility’, and ‘carcasses’. After carefully evaluation, the final dataset consisted 35 in-vivo studies comprising 964 treatment units. The data analysis and coding were performed using software R version 4.2.1 ‘Funny-looking kid’ computing with library mode (cowplot); (tidyverse); and (viridis); and (nlme). The results showed the level of probiotics increased body-weight gain with a linear pattern (p

Objective Our objective was to examine the relationships of supplemental rumen-protected lysine (RPL) or lysine + methionine (RPLM) on lactational performance, plasma amino acids (AA) concentration, and nitrogen use efficiency of lactating dairy cows by using a meta-analysis approach. Methods A total of 56 articles comprising 77 experiments with either RPL or RPLM supplementation were selected and analyzed using a mixed model methodology by considering the treatments and other potential covariates as fixed effects and different experiments as random effects. Results In early lactating cows, milk yield was linearly increased by RPL (β1 = 0.013; p< 0.001) and RPLM (β1 = 0.014; p

One of the promising ways to enhance resistant starch (RS), which is safer and available in food-grade form, is to use organic acids. Several studies have reported the effect of the organic acids (OAs) intervention on RS content of foods, but the results have not been quantitatively summarized yet. Therefore, this study aimed to evaluate such OAs interventions using a meta-analysis method. A total of 18 articles with 86 comparisons between the control and experimental (OAs intervention) groups were included as a database in this meta-analysis. The results revealed that the modification of RS with OAs significantly decreased (P tubers > cereals. The RS content of both the flour and starch samples was enhanced (P

The two most extensively beekeeping honeybee species were Apis mellifera and A. cerana. Other species that produced honey, albeit with infrequent cultivation, include A. dorsata, A. florea, and some stingless bees, including Trigona and Melipona. Different types of honeybees were known to affect the quality of honey. Hence, this investigation aimed to conduct a meta-analysis to examine the similarities in honey quality between honey sourced from beekeeping and wild honeybees. Data analysis was performed using the OpenMEE software, facilitating the calculation of effect size and standard error. The effect size and common error data were subsequently organized into separate columns within a CSV file. This CSV file was then imported into the JASP 0.16.2 software to conduct heterogeneity and Egger tests to detect potential publication bias. The findings indicated significant disparities in the quality of honey produced by beekeeping, wild, and stingless bee honey, as determined by various parameters, including pH value, moisture, total sugar, acidity, HMF (hydroxymethylfurfural), and diastase enzyme levels (p < 0.05). According to the results of the meta-analysis, honey from beekeeping exhibited superior quality to that of wild and stingless bees. However, the average values of all parameters still adhered to the established honey quality standards set by the Standar Nasional Indonesia and the International Honey Standard (IHS). Keywords: honey, honeybees, meta-analysis, physicochemical quality

This study evaluated the effects of arbuscular mycorrhizal fungi (AMF) on plant growth, nutrient uptake, and heavy metal accumulation on polluted land using a meta-analysis approach. Data from 33 relevant studies were selected based on inclusion criteria, specifically articles in English, observational research, and investigating the role of AMF in plant growth and productivity on polluted land. The results showed that plants inoculated with AMF experienced significant accumulation of heavy metals in roots, such as Pb (p

A meta-analysis was conducted to determine the effects of herbal plant extracts on the growth performance, blood parameters, nutrient digestibility and carcase quality of farmed rabbits. A dataset was created from 33 in vivo studies comprising 121 experimental units. Statistical meta-analysis was performed using a random-effects model and linear-mixed model meta-regression using R software (v. 4.3.0). Our results showed that although supplemental herbs did not affect average daily gain (ADG) and final body weight (BW), they reduced (P

Antara, the official news agency of the state, reported a record-breaking population of 303 Bali starlings in the West Bali National Park (WBNP) in June 2020, attributing this achievement to the park's captive reproduction initiative. This paper presents a study on the dynamic equilibrium of Bali Starlings and proposes a mathematical model for analyzing this dynamic. The research also examines parameters ensuring the stability of the captive breeding model for Bali starlings in WBNP in a sustainable manner. The Bali starlings are categorized into two groups: those in the wild and those in captive breeding, with hatched eggs in captivity included in the latter. The dynamic model is analyzed for system stability around the endemic critical point using the Routh-Hurwitz stability criteria. As an illustrative example, a simulation is conducted to assess the model's suitability under real field conditions. The model analysis reveals that the existence of an endemic critical point can be maintained if the percentage of stolen Bali starlings or eggs reintroduced to the wild is less than the difference between the percentage of Bali starlings laying eggs and the population growth rate in WBNP. Furthermore, the stability of the endemic critical point is confirmed as long as the percentage of Bali starlings laying eggs exceeds the population growth rate. This dynamic model offers a valuable tool for evaluating the sustainability of Bali starling breeding programs and optimizing the benefits associated with their conservation efforts.

Objective The present study aimed to evaluate the effects of extruded and unextruded feeding on the performance, milk composition, digestibility and ruminal fermentation of dairy cows through a meta-analysis. Methods The database was compiled from 53 studies in Scopus and PubMed. The data were analyzed using a random effects model in OpenMEE software. Extruded feed was grouped as the experiment group while and the others as control group. The bias of publication in the main parameter of dairy performance was evaluated by a funnel plot. Results The result showed that extruded feed enhanced the milk yield, dry matter and crude protein digestibility, butyrate and valerate acid production (p

Transportation is a very important field of activities in the life of Indonesian people. Recognizing the importance role of transportation, traffic and road transportation must be organized in an integrated national transportation system and able to realize the availability of transportation services in accordance to the level of need. One of the most widely used transportation is ojek online. Of course, some factors become the influence of the use of online motorcycle taxis. One quantitative method that can measure customer perception using online motorcycle taxis is the Factor Analysis method, which is a statistical analysis used to find out the factors that underlie and show interrelationships between changemakers. Based on questionnaires distributed to 150 students in the FMIPA environment of Udayana University in 2021 and after an analysis of factors on questionnaire data, it was obtained that factors which influence students' decisions to use online motorcycle taxis are location and destination factors, service factors, application factors, and promotional factors. These factors can explain the diversity of students to use online motorcycle taxis by 34.666%; 28,897%; 22.563% and 10.873%. The dominant factor that mostly influence students' decision to use online motorcycle taxis is the factor of place and destination location that can be explained by 34.666%.

Background and Aim: Herbal plants have the potential to reduce the population of metagonic bacteria and protozoa due to the bioactive compound contained in herbal plants. This study aimed to evaluate the effect of herbal plant supplementation on rumen fermentation characteristics, methane (CH4) gas emissions, in vitro nutrient digestibility, and protozoan populations. Materials and Methods: This study consisted of two stages. Stage I involved determining the potential of herbal plants to increase total gas production (Orskov and McDonald methods) and reduce the protozoan population (Hristov method). Three potential herbs were selected at this stage and used in Stage II as supplements in the palm kernel cake (PKC)-based diet (30% herbal plants + 70% PKC). Proximate and Van Soest analyses were used to determine the chemical composition. In vitro dry matter digestibility (IVDMD), organic matter (IVOMD), and rumen fermentation characteristics were determined using Theodorous method. Conway microdiffusion was used to determine ammonia concentration (NH3). Gas chromatography was used to determine the total and partial volatile fatty acid production. Results: The results of the first stage showed that seven herbal plants (Moringa oleifera, Rhodomyrtus tomentosa, Clerodendron serratum, Curcuma longa Linn., Urena lobata, Uncaria, and Parkia timoriana) significantly differed in terms of total gas production (p < 0.05). Herbal plants can increase gas production and reduce protozoan populations. The highest total gas production was observed using P. timoriana, M. oleifera, and C. longa Linn. Moringa oleifera plants were the most effective in lowering protozoa population. In Stage 2, the supplementation of herbal plants in PKC-based-diet significantly increased IVDMD, that was ranged from 56.72% to 65.77%, IVOMD that was ranged from 52.10% to 59.54%, and NH3, that was ranged from 13.20 mM to 17.91 mM. Volatile fatty acid partial and total gas production potential and CH4> gas emissions were also significantly different from those of the control (p < 0.05). Conclusion: Supplementation of M. oleifera, C. longa Linn., and P. timoriana in ruminant diet effectively increased total gas production, IVDMD percentage, and IVOMD, and reduced CH4 gas emissions and protozoa populations during rumen fermentation.

The effect of using saponins on ruminants' performance differed from several published research data based on the level of saponins added to the feed. This research was conducted to analyze the effect of saponins on fermentation characteristics, digestibility, and estimation of methane in ruminants with a mixed model approach from published journal articles—a total of 127 studies from 32 journals national and international. The variable measured included the level of saponins (%), dry matter intake, Average Daily Gain (ADG), Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Crude protein (CP), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), protozoa population, bacterial population, Volatile Fatty Acid (VFA), acetate/C2, propionate/C3, butyrate/C4, valerate/C5, acetate/propionate (C2/C3), NH3, pH, and methane gas production. The results showed that using saponins in ruminants increased ADG, CP, ADF, NDF degradation, Total VFA, and proportion of propionate. The addition of saponins level reduced the protozoa population, acetate proportion, and the ratio of acetate: to propionate (C2/C3). In contrast, feed intake and digestibility decreased with the administration of saponin. The bacterial population were similar among treatments, and methane production increased by increasing saponins. In conclusion, the administration of saponin level recommended is 0.3-3.1% of the total ration to improve performance and increase feed efficiency.

Objective This study was conducted to evaluate the use of Megasphaera elsdenii (M. elsdenii) as a probiotic on rumen fermentation, production performance, carcass traits and health of ruminants by integrating data from various related studies using meta-analysis. Methods A total of 32 studies (consisted of 136 data points) were obtained and integrated into a database. The parameters integrated were fermentation products, rumen microbes, production performance, carcass quality, animal health, blood and urine metabolites. Statistical analysis of the compiled database used a mixed model methodology. Different studies were considered random effects, while M. elsdenii supplementation doses were considered fixed effects. p-values and the Akaike information criterion were employed as model statistics. The model was deemed significant at p

Bangun ruang lingkaran merupakan salah satu bentuk bangun ruang yang sering digunakan dalam aplikasi teknik dan industri, sepeti pada mesin turbin, pompa dan perpipaan. Saat benda yang berbentuk linkaran bergerak didalam aliran fluida, maka akan mengalami gaya hambat atau drag yang dapat mempengaruhi kinerjanya. Penelitian ini bertujuan untuk mempelajari pengaruh variasi kecepatan aliran fluida terhadap nilai koefisien drag pada bangun ruang lingkaran sesuai dengan standar ASME PTC 16. Pengujian dilakukan dengan menggunakan model skala yang terbuat dari bahan kayu berbentuk lingkaran dengan diameter 6 cm dan panjang 20,5 cm. Pengujian dilakukan di laboratorium mekanika fluida dengan menggunakan alat wind tunnel pada variasi kecepatan aliran fluida 30 m/s dan 40 m/s dan dilakukan simulasi dengan menggunakan aplikasi Ansys. Hasil pengujian menunjukkan bahwa nilai koefisien drag meningkat seiring dengan peningkatan kecepatan aliran fluida. Tekanan terukur yang dihasilkan pada kecepatan aliran fluida 30 m/s pada menit ke 20 sebesar 2,8 mmH2O sedangkan pada kecepatan aliran fluida 40 m/s sebesar 5,2 mmH2O. Distribusi kecepatan fluida pada permukaan lingkaran menunjukkan bahwa adanya turbulensi pada kecepatan aliran fluida yang tinggi. Olehnya itu semakin tinggi kecepatan aliran fluida maka semakin besar kemungkinan terjadinya turbulensi.

The increase in electricity consumption goes hand in hand with the growth of population and industry. Sustainable power plants such as turbines are one option. Turbines and generators are the main components as well as the availability of sufficient water discharge to turn the turbine so that it can be forwarded to the generator. This research was conducted in order to test the performance of a turbine laboratory scale that has been designed. The output power generated in the crossflow turbine is 12.5 Watt to 287.906 Watt for a load of 100 Watt and a variable guide blade with a static head of 0.25 kg/cm

The present meta-analysis study was aimed to determine effects of essential oil and their derivatives (EOD) supplementation as natural additives on rumen fermentation characteristics and rumen biohydrogenation (BH) activity in vitro. A meta-analysis database was built from the 28 verified scientific articles and further all data were analysed through the continuous random effects model using OpenMEE. It was evaluated that the EOD levels up to 500 mg/L. It showed that high EOD levels increased pH and acetate concentration (P < 0.001) but reduced ammonia, propionate, the acetate to propionate ratio, total volatile fatty acid, and rumen gas production in vitro (P < 0.001). On the perspective of rumen fatty acids profile, the increased EOD levels also positively accumulating n-9 monounsaturated fatty acid, conjugated linoleic acid c9 t11, as well as n-6 and n-3 polyunsaturated fatty acid (PUFA) in the rumen after in vitro fermentation (P < 0.001), and consequently inhibited rumen biohydrogenation (BH) of n-6 and n-3 PUFA (P < 0.001). Meta-regression analysis also showed a negative correlation between EOD supplementation levels and the BH of C18:2 n-6, C18:3 n-3, C20:5 n-3, and C22:6 n-3. It was suggested that the EOD supplementation at 300 mg/L indicated a positive effect on modulating the rumen PUFA metabolism. Above all evidences, it can be concluded that EOD treatment can reduce rumen fermentability but increase the proportion of beneficial fatty acids and inhibit the rate of BH PUFA.

Last survivor insurance is life insurance for two or more participants with premiums paid up to the death of the last participant. This study discusses term last survivor insurance for two participants, namely husband and wife. Compensation money is paid after the second person dies. The purpose of this study is to determine the value of annual premiums and benefit reserves in last survivor term insurance with non-constant premiums using a retrospective calculation method. The conclusion obtained for the annual premium model is not constant, the 10-year term last survivor insurance with an annual premium change of 10% of the fixed premium( is that during the first 5 years, premiums with ? negative are always greater than premiums with ? positive . But the opposite is true for the next 5 years. then, the value of reserves in the same year with ? negative is greater than ? positive, reserves with ? positive and ? negative also have the same pattern, namely increasing in the first year to the 6th year then decreasing in the 7th year until the contract expires.

Since the Covid-19 pandemic, Indonesian tourism has experienced a drastic decline. This decline can be seen in the number of foreign tourists visiting Indonesia. The number of foreign tourist arrivals in 2020 and 2021 is far less compared to 2019 before Covid-19 entered. As a result, the Indonesian economy also suffered. Regarding the recovery of Indonesian tourism after the pandemic has been slow down, this study aims to cluster foreign tourists visiting Indonesia based on the amount of their expenditures and length of stays using the K-means algorithm. Secondary data from National Statistics Bureau classified the origin of tourists were 86 countries. Applying k-means algorithm methods to cluster country of origin, result showed they were three clusters formed based on the attributes of visiting, i.e. length of stay in Indonesia and total amount of their expenditures. Each cluster consists of 14, 54 and 18 countries. The first cluster is characterized by countries that have high tourism spending; the second cluster is formed by countries with moderate tourism spending; and the third cluster is characterized by countries with low tourism spending. The accuracy of the three clusters in explaining the variance of tourist spending is 68.8 percent.

Strongyloides have been known to infect many hosts, including humans and animals around the world. The two, S. stercoralis and S. fuellborni, were zoonotic species that cause human strongyloidiasis. The disease induces a hyper infection syndrome in an immunocompetence person, while the clinical signs of infections in animals vary from asymptomatic to sudden death in severe and fatal cases. However, integrated data on Strongyloides infection among human and animal hosts in Indonesia is sparse. The present study aims to provide information on the prevalence of Strongyloides concerning host type and geographical region in Indonesia. Literature searches were conducted to identify epidemiological data on the occurrence of Strongyloides in humans and animals from 1985-2022 in seven regions of Indonesia, including 1) Sumatra; 2) Java; 3) Kalimantan; 4) Bali and Nusa Tenggara; 5) Sulawesi; 6) Maluku; and 7) Papua. The data were described and mentioned as percent prevalence and confidence intervals (CIs) of 95%. A total of 46 articles confirmed the Strongyloides infection in Indonesia during 1985-2022. The articles consisted of nine on humans (12 data) and 37 on animals (59 data). Human strongyloidiasis could be confirmed in five regions (Bali and Nusa Tenggara, Kalimantan, Maluku, Papua, and Sulawesi). Strongyloides infected some animal group hosts, such as pigs, ruminants (beef cattle, swamp buffalo, sheep, and goat), rodents, nonhuman primates (orangutan, lutung (Trachypithecus auratus), and long-tailed macaque), and others (rabbit, snake, and chicken) in different regions. This study provides the prevalence data on Strongyloides infection in human and animal hosts. The limited data on Strongyloides in Indonesia indicated that the disease is still being neglected and under-reported. More epidemiological studies with the improved diagnostic method are needed to determine the societal burden of the parasites.

Interest has recently grown in the research of humic substances (HS) as a potential aquaculture feed additive, resulting in a growing number of publications. However, previous studies have shown varying results. Therefore, to gain a comprehensive understanding of the effects of HS, a quantitative analysis conducted through a meta-analysis is required. This study employed a meta-analysis approach to evaluate the effect of HS as a feed additive on the specific growth rate (SGR) and feed conversion ratio (FCR) of fish aquaculture. The Preferred Reporting Items for Systematic and Meta-Analysis (PRISMA) method was used to collect the data. Multiple databases were searched to identify relevant articles from scholarly journals. Ultimately, 18 publications that met the requirements were included in the analysis using OpenMEE software. The results revealed an effect size (comparing the control and the HS-supplemented groups) of 0.154 (95 % CI, P < 0.001) for SGR. Additionally, there was a notable improvement in FCR with an effect size of -0.220 (95 % CI, P < 0.001). However, it is important to note that the analysis showed high heterogenicity (I2 values of 98.87 % and 97.91 % for SGR and FCR, respectively). In conclusion, HS supplementation may significantly improve fish growth and reduce FCRs in aquaculture. This meta-analysis contributes valuable insight into the use of HS as a beneficial feed additive in aquaculture practices.

The current meta-analysis aimed to estimate the effect of different selenium (Se) sources on immune organs, plasma immunoglobulins, blood profiles, and broiler performances. Related studies that met standard presence criteria were identified and mined from the Scopus database. The database was developed from 38 articles. Data were analyzed using the OpenMEE, considering the difference between organic selenium as fixed effects and different studies as random effects. This study showed that organic selenium feed supplementation improved the feed conversion ratio and increased the average daily feed intake and gain of broilers. Furthermore, the mortality of broilers fed organic Se was significantly lower than that of those fed inorganic Se. For the immune organ of the broiler, organic selenium feed supplement enhanced the thymus and spleen organs but did not affect the bursa organ. IgA and IgM were significantly higher in the broilers fed organic selenium feed; meanwhile, IgG of broilers fed organic selenium was lower than those fed inorganic selenium. The total protein blood concentration of broilers fed organic selenium was significantly higher than those fed inorganic Se. Meanwhile, there was no statistically significant difference in the effects of selenium source on cholesterol. Triglyceride concentrations of broilers fed organic Se are significantly lower than those fed inorganic Se. The ratio of heterophile to lymphocyte in broilers fed organic Se is significantly lower than in those fed inorganic selenium. In conclusion, the organic selenium feed supplement can promote production performance and immune parameters of broilers.

Type 2 diabetes mellitus (DMT2) is a metabolic disease that is increasingly attracting public attention. Diabetes mellitus is expected to reach 439 million in the world in 2030. Resistant starch (RS) is an indigestible starch which has health properties which has health properties that can be used for preventing diabetes mellitus type 2. In order to increase the RS content, a dual modification method consisted of acidification and heat moisture treatment (HMT) can be applied. The Acid-HMT method is affected by various factors, i.e., acid types, acid concentration, water content ratio, HMT temperature and HMT processing time, and different treatments may result in different RS yields. This study aimed to analyze the effective treatment in the Acid-HMT dual modification to enhance RS content by using a systematic review based on the PRISMA method. The studies revealed that there were 11 articles (n = 68 data) which utilized various acid types combined with HMT. The utilization of acid-alcohol, HCl, and organic acid such as citric acid, acetic acid, and lactic acid resulted in different results of RS content in modified starch. In addition to acid types, treatment conditions such as acid concentration, acidification time, acidification temperature, water content ratio, HMT time, and HMT temperature also affected the resulted RS. The treatment with 0.2 M citric acid for 24 hours at 25 ℃ combined with HMT with 30% moisture at 110 ℃ for 8 hours resulted in the highest increase in RS content of modified starch.

Aflatoxins can easily grow and develop in many feed ingredients and influence the risk of several animal chronic diseases. The present study aimed to evaluate the effect of bentonite inclusion on performances, carcass yield, giblet, and blood constituents of broiler-fed contaminated aflatoxin through a meta-analytical approach. A database was developed based on scientific publications that were searched using several search tools such as Science Direct, PubMed, Scopus, and Google Scholar with “bentonite”, “clay”, “montmorillonite”, “aflatoxin” and “broiler” as keywords. A total of 31 studies were retrieved and included in the analysis. Data analysis was based on the mixed model in which dietary bentonite inclusion was treated as the fixed effect and different studies were considered as random effects. Bentonite used were sodium bentonite, calcium bentonite, and montmorillonite form with levels ranging from 0 to 5%. Meanwhile, aflatoxin B1 levels in the diet ranged from 0 to 5 mg/kg. In this study, body weight gain, feed intake, and feed efficiency showed a linear increase (p

The present study aimed to evaluate the use of biochar as a feed additive on nutrient utilization and livestock performance by employing a meta-analysis method. Various in vitro and in vivo studies regarding the effects of biochar on rumen fermentation, nutrient disappearance, total gas, methane production, microbial population, feed intake, growth performance, blood constituents, nutrient digestibility and nitrogen retention were tabulated in a database. Data were analysed using the mixed model method in which the different studies were considered as random effects while the biochar addition was treated as a fixed effect. The addition of biochar reduced methane production (quadratic pattern; P < 0.05), but increased the total gas production (P < 0.001). Addition of biochar decreased (quadratic pattern; P < 0.05) volatile fatty acids and acetic acid in the in vitro rumen fermentation data. In the in vivo data, a reduction in feed conversion ratio (quadratic pattern; P < 0.05), as well as a reduction (P

The present meta-analysis aimed to determine the underlying effects of different saponins extracted from different sources on the production performance, milk yield, digestibility, rumen fermentation, blood metabolites, and nitrogen utilization of ruminants. A total of 26 papers comprising 66 in vivo studies (148 data points of dietary treatments) were evaluated in the present study. The databases were statistically analyzed using the mixed model procedure of SAS, where experiments considered random effects and tannin-related factors were treated as fixed effects. Statistical procedures were then continued in comparing different sources of saponin extract through Mixed Model analysis, where experiments were also random factors and sources of saponin extract were fixed factors. The evidence revealed in the present meta-analysis that saponin supplementation of up to 40 g/kg DM appears to have no detrimental impact on feed intake across ruminant types, suggesting that it does not significantly affect diet palatability. However, the results indicated that there are species-specific responses to saponin supplementation, particularly in relation to palatability and nutrient absorption efficiency, with larger ruminants being better able to tolerate the bitterness induced by saponin extracts. Furthermore, the study found that saponin extracts can influence nutrient digestibility and rumen fermentation dynamics, with different effects observed in large and small ruminants. While some saponin extracts can enhance average daily weight gain and milk yield, others can have adverse effects, highlighting the importance of considering both saponin sources and animal physiological condition when developing nutritional strategies. Additionally, optimization of ruminant production by utilizing saponin extracts is necessary to avoid negative health implications, such as increased blood creatinine levels. Different saponin extracts utilization in ruminant nutrition and environmental management, have a distinct understanding associated to their various bioactive properties. However, among the saponin sources, saponin extracted from Quilaja saponaria is more likely to improve large ruminant production performance while maintaining ruminant health and metabolism, but negatively affect small ruminants. Further research is needed to unravel the intricate effects of different saponin sources on ruminant health and productivity, emphasizing the importance of tailored dietary strategies that consider the unique physiological and metabolic characteristics of the target livestock.