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Charles University in Prague Faculty of Pharmacy in Hradci Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Markéta Jeřábková Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Copper reducing effects of flavones Free radicals are commonly present in our organism, however, in case of their accumulation it could cause many diseases. Copper is an important trace element which is essential for many biological processes. On the other hand, copper can catalyze processes which produce damaging radicals. Flavonoids are polyphenolic compounds with many biological effects. The most important is antioxidant activity which protects us from free radicals. Flavonids can also have a prooxidant effect. This thesis was focused on a detection of the reducing activity of flavones (mosloflavone, luteolin, apigenin, crysin, diosmin, 5-hydroxyflavone, baicalin, baicalein, negletein, apigenin-7-glucoside) at different pH. A reducing activity of surveyed flavones was taken by the methodics of spectrophotometric analysis with the use of a selective indicator of cuprous ionts formed in bathocuproinedisulfonic acid disodium salt at different pH. A reducing activity depends on the structure of flavones and pH environment. From the definition of the relation between the structure of a flavone...

Charles University in Prague Faculty of Pharmacy in Hradci Králové Department of Pharmaceutical botanic and ecology Candidate: Markéta Jeřábková Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Copper chelating activity of flavonols Copper belongs to essential trace elements. It is involved in different biological processses essential for life therefore metabolism disorders of copper can give rise to patological displays. A raised level of loose copper in serum at some diseases arouses a great interest in the "chelating" therapy and the study of new potential chelators. The importance of chelation is based on the maintenance of copper homeostasis and the elimination of its abundance. It serves the intoxication therapy of copper and other heavy metals and the treatment of Wilson's disease. Flavonoids are polyphenolic compounds with antioxidant effects. Flavonoids able to chelate some metal ions through which they reduce the possibility of the production of a hydroxyl radical which decrease the effect of oxidative stress on organism. The possible use of the "chelating" therapy with flavonoids is investigated also at cardiovascular and neurodegenerative diseases, the occurrence of cancer and the inflammation. This diploma thesis was focused on discovering chelating activity of flavonols:...