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1. LPIAT, a lyso-Phosphatidylinositol Acyltransferase, Modulates Seed Germination in Arabidopsis thaliana through PIP Signalling Pathways and is Involved in Hyperosmotic Response

Author

Denis Coulon, Lionel Faure, Magali Grison, Stéphanie Pascal, Valérie Wattelet-Boyer, Jonathan Clark, Marina Le Guedard, Eric Testet, and Jean-Jacques Bessoule

Subjects
acyltransferase, phosphoinositides, seed germination, hyperosmotic stress, aba, aleurone-like cells, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

Lyso-lipid acyltransferases are enzymes involved in various processes such as lipid synthesis and remodelling. Here, we characterized the activity of an acyltransferase from Arabidopsis thaliana (LPIAT). In vitro, this protein, expressed in Escherichia coli membrane, displayed a 2-lyso-phosphatidylinositol acyltransferase activity with a specificity towards saturated long chain acyl CoAs (C16:0- and C18:0-CoAs), allowing the remodelling of phosphatidylinositol. In planta, LPIAT gene was expressed in mature seeds and very transiently during seed imbibition, mostly in aleurone-like layer cells. Whereas the disruption of this gene did not alter the lipid composition of seed, its overexpression in leaves promoted a strong increase in the phosphatidylinositol phosphates (PIP) level without affecting the PIP2 content. The spatial and temporal narrow expression of this gene as well as the modification of PIP metabolism led us to investigate its role in the control of seed germination. Seeds from the lpiat mutant germinated faster and were less sensitive to abscisic acid (ABA) than wild-type or overexpressing lines. We also showed that the protective effect of ABA on young seedlings against dryness was reduced for lpiat line. In addition, germination of lpiat mutant seeds was more sensitive to hyperosmotic stress. All these results suggest a link between phosphoinositides and ABA signalling in the control of seed germination

Published
2020
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2. Proteomic Analysis of Lipid Droplets from Arabidopsis Aging Leaves Brings New Insight into Their Biogenesis and Functions

Author

Lysiane Brocard, Françoise Immel, Denis Coulon, Nicolas Esnay, Karine Tuphile, Stéphanie Pascal, Stéphane Claverol, Laëtitia Fouillen, Jean-Jacques Bessoule, and Claire Bréhélin

Subjects
lipid droplet, leaf senescence, label free proteomics, Small rubber particle protein1, electron tomography, ER contact site, Plant culture, SB1-1110
Abstract

Lipid droplets (LDs) are cell compartments specialized for oil storage. Although their role and biogenesis are relatively well documented in seeds, little is known about their composition, structure and function in senescing leaves where they also accumulate. Here, we used a label free quantitative mass spectrometry approach to define the LD proteome of aging Arabidopsis leaves. We found that its composition is highly different from that of seed/cotyledon and identified 28 proteins including 9 enzymes of the secondary metabolism pathways involved in plant defense response. With the exception of the TRIGALACTOSYLDIACYLGLYCEROL2 protein, we did not identify enzymes implicated in lipid metabolism, suggesting that growth of leaf LDs does not occur by local lipid synthesis but rather through contact sites with the endoplasmic reticulum (ER) or other membranes. The two most abundant proteins of the leaf LDs are the CALEOSIN3 and the SMALL RUBBER PARTICLE1 (AtSRP1); both proteins have structural functions and participate in plant response to stress. CALEOSIN3 and AtSRP1 are part of larger protein families, yet no other members were enriched in the LD proteome suggesting a specific role of both proteins in aging leaves. We thus examined the function of AtSRP1 at this developmental stage and found that AtSRP1 modulates the expression of CALEOSIN3 in aging leaves. Furthermore, AtSRP1 overexpression induces the accumulation of triacylglycerol with an unusual composition compared to wild-type. We demonstrate that, although AtSRP1 expression is naturally increased in wild type senescing leaves, its overexpression in senescent transgenic lines induces an over-accumulation of LDs organized in clusters at restricted sites of the ER. Conversely, atsrp1 knock-down mutants displayed fewer but larger LDs. Together our results reveal that the abundancy of AtSRP1 regulates the neo-formation of LDs during senescence. Using electron tomography, we further provide evidence that LDs in leaves share tenuous physical continuity as well as numerous contact sites with the ER membrane. Thus, our data suggest that leaf LDs are functionally distinct from seed LDs and that their biogenesis is strictly controlled by AtSRP1 at restricted sites of the ER.

Published
2017
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3. JulyNonenzymatic synthesis of glycerolipids catalyzed by imidazole

Author

Eric Testet, Malika Akermoun, Miyuki Shimoji, Claude Cassagne, and Jean-Jacques Bessoule

Subjects
acyl-CoA, lysolipid, acylation, chemical method, omega hydroxypalmitoyl-CoA, Biochemistry, QD415-436
Abstract

Imidazole catalyzed acylations of lysolipids by acyl-CoAs in water at room temperature and at a pH close to neutrality. In the presence of oleoyl-CoA and either lysophosphatidylcholine, 1-palmitoyl-sn-glycero-3-phosphocholine (LPC); lysophosphatidylglycerol, monoacyl-sn-glycero-3-phosphoglycerol; lysophosphatidyl acid, 1-oleoyl-sn-glycero-3-phosphate; lysophosphatidylserine, monoacyl-sn-glycero-3-phosphoserin; or lysophosphatidylethanolamine, monoacyl-sn-glycero-3-phosphoethanolamine, the corresponding phospholipids were synthesized. Similarly, the use of lyso-platelet activating factor, an ether analog of LPC, yielded the formation of 1-O-alkyl-2-oleoyl-sn-glycero-3-phosphocholine. In the presence of LPC, an imidazole-catalyzed synthesis of phosphatidylcholine (PC) occurred when medium, long, and very long chain acyl-CoAs were added. With hydroxyacyl-CoA, a similar PC synthesis was obtained.The process described in the present paper appears to offer several potential applications of interest for the synthesis of glycerophospholipids and triglycerides with labeled and/or an unusual or fragile fatty acid, or when suitable acyltransferases have not yet been described in the literature and/or are not commercially available. The method described is very safe and simple since lipids can be synthesized in tubes containing 0.7% imidazole in water, and left for a few hours at room temperature on the bench.

Published
2002
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4. A Fox2-dependent fatty acid ß-oxidation pathway coexists both in peroxisomes and mitochondria of the ascomycete yeast Candida lusitaniae.

Author

Frédéric Gabriel, Isabelle Accoceberry, Jean-Jacques Bessoule, Bénédicte Salin, Marine Lucas-Guérin, Stephen Manon, Karine Dementhon, and Thierry Noël

Subjects
Medicine, Science
Abstract

It is generally admitted that the ascomycete yeasts of the subphylum Saccharomycotina possess a single fatty acid ß-oxidation pathway located exclusively in peroxisomes, and that they lost mitochondrial ß-oxidation early during evolution. In this work, we showed that mutants of the opportunistic pathogenic yeast Candida lusitaniae which lack the multifunctional enzyme Fox2p, a key enzyme of the ß-oxidation pathway, were still able to grow on fatty acids as the sole carbon source, suggesting that C. lusitaniae harbored an alternative pathway for fatty acid catabolism. By assaying 14Cα-palmitoyl-CoA consumption, we demonstrated that fatty acid catabolism takes place in both peroxisomal and mitochondrial subcellular fractions. We then observed that a fox2Δ null mutant was unable to catabolize fatty acids in the mitochondrial fraction, thus indicating that the mitochondrial pathway was Fox2p-dependent. This finding was confirmed by the immunodetection of Fox2p in protein extracts obtained from purified peroxisomal and mitochondrial fractions. Finally, immunoelectron microscopy provided evidence that Fox2p was localized in both peroxisomes and mitochondria. This work constitutes the first demonstration of the existence of a Fox2p-dependent mitochondrial β-oxidation pathway in an ascomycetous yeast, C. lusitaniae. It also points to the existence of an alternative fatty acid catabolism pathway, probably located in peroxisomes, and functioning in a Fox2p-independent manner.

Published
2014
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5. Phosphoinositides containing stearic acid are required for interaction between Rho <scp>GTPases</scp> and the exocyst to control the late steps of polarized exocytosis

Author

Patricia Laquel, Eric Testet, Karine Tuphile, Christophe Cullin, Laetitia Fouillen, Jean‐Jacques Bessoule, and François Doignon

Subjects
rho GTP-Binding Proteins, Saccharomyces cerevisiae Proteins, Structural Biology, Vesicular Transport Proteins, Genetics, Saccharomyces cerevisiae, Cell Biology, Phosphatidylinositols, Molecular Biology, Biochemistry, Exocytosis, Stearic Acids
Abstract

Cell polarity is achieved by regulators such as small G proteins, exocyst members and phosphoinositides, with the latter playing a key role when bound to the exocyst proteins Sec3p and Exo70p, and Rho GTPases. This ensures asymmetric growth via the routing of proteins and lipids to the cell surface using actin cables. Previously, using a yeast mutant for a lysophosphatidylinositol acyl transferase encoded by the PSI1 gene, we demonstrated the role of stearic acid in the acyl chain of phosphoinositides in cytoskeletal organization and secretion. Here, we use a genetic approach to characterize the effect on late steps of the secretory pathway. The constitutive overexpression of PSI1 in mutants affecting kinases involved in the phosphoinositide pathway demonstrated the role of molecular species containing stearic acid in bypassing a lack of phosphatidylinositol-4-phosphate (PI(4)P) at the plasma membrane, which is essential for the function of the Cdc42p module. Decreasing the levels of stearic acid-containing phosphoinositides modifies the environment of the actors involved in the control of late steps in the secretory pathway. This leads to decreased interactions between Exo70p and Sec3p, with Cdc42p, Rho1p and Rho3p, because of disruption of the GTP/GDP ratio of at least Rho1p and Rho3p GTPases, thereby preventing activation of the exocyst.

Published
2022

9. Lyso-Phosphatidic Acid Acyl-Transferases: a link with intracellular protein transport in Arabidopsis root cells?

Author

Dittrich-Domergue F, Jean-Jacques Bessoule, Le-Guedard M, Lilly Maneta-Peyret, Patrick Moreau, Wattelet-Boyer, Yohann Boutté, and Kriechbaumer

Subjects
chemistry.chemical_compound, biology, Biochemistry, Chemistry, Mutant, Lysophosphatidic acid, Arabidopsis thaliana, Aquaporin, Endomembrane system, Phosphatidic acid, biology.organism_classification, Secretory pathway, Transport protein
Abstract

Phosphatidic acid (PA) and Lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (numbered 2,3,4 and 5) were identified in A. thaliana. These AtLPAATs, displaying an enzymatic activity specific for LPA to produce PA, are located in the endomembrane system. We focused on the putative role of the AtLPAATs 3, 4 and 5 in the secretory pathway of root cells through genetical (knock-out mutants), biochemical (activity inhibitor, lipid analyses) and imaging (live and immuno-confocal microscopies) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 showed a primary root growth decrease. The transport of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, but not that of H+-ATPases. The lpaat4;lpaat5 double mutant was sensitive to salt stress and the transport of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein transport impairment was due to a critical PA concentration threshold.HighlightPhosphatidic acid produced by Lyso-Phosphatidic Acid Acyl-Transferases has an impact on the efficiency of the intracellular transport of some proteins in Arabidopsis thaliana root cells.

Published
2021

10. Lysophosphatidic acid acyltransferases: a link with intracellular protein trafficking in Arabidopsis root cells?

Author

Valérie Wattelet-Boyer, Marina Le Guédard, Franziska Dittrich-Domergue, Lilly Maneta-Peyret, Verena Kriechbaumer, Yohann Boutté, Jean-Jacques Bessoule, and Patrick Moreau

Subjects
Protein Transport, Indoleacetic Acids, Physiology, Arabidopsis Proteins, Arabidopsis, Plant Science, Acyltransferases
Abstract

Phosphatidic acid (PA) and lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (LPAAT2, 3, 4, and 5) were identified in Arabidopsis thaliana. These AtLPAATs display a specific enzymatic activity converting lysophosphatidic acid to PA and are located in the endomembrane system. We investigate a putative role for AtLPAATs 3, 4, and 5 in the secretory pathway of root cells through genetical (knockout mutants), biochemical (activity inhibitor, lipid analyses), and imaging (live and immuno-confocal microscopy) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 produced a significant decrease in primary root growth. The trafficking of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, whereas trafficking of H+-ATPases was unaffected. The lpaat4;lpaat5 double mutant is sensitive to salt stress, and the trafficking of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein trafficking impairment was due to a critical PA concentration threshold.

Published
2021

11. High temperature patterns at the onset of seed maturation determine seed yield and quality in oilseed rape (Brassica napus L.) in relation to sulphur nutrition

Author

Marina Le Guédard, Lethicia Magno Massuia de Almeida, Annette Morvan-Bertrand, Tae-Hwan Kim, Alain Mollier, Marie-Hélène Wagner, María Reyes González-Centeno, Jean-Christophe Avice, Sophie Brunel-Muguet, Pierre-Louis Teissedre, Jean-Jacques Bessoule, Ecophysiologie Végétale, Agronomie et Nutritions NCS (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), GEVES Station nationale d'essais de semences, Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), LEB Aquitaine Transfert-ADERA, Yonsei University, Interactions Sol Plante Atmosphère (UMR ISPA), Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Station Nationale d’Essais de Semences (SNES), and Chonnam National University [Gwangju]

Subjects
0106 biological sciences, 0301 basic medicine, oilseed rape, [SDV]Life Sciences [q-bio], Brassica, chemistry.chemical_element, Plant Science, 01 natural sciences, thermotolerance, Desiccation tolerance, Crop, high temperature, 03 medical and health sciences, repeated stresses, seed quality, priming, stress memory, Ecology, Evolution, Behavior and Systematics, 2. Zero hunger, biology, Seed dormancy, food and beverages, biology.organism_classification, Temperature stress, Sulfur, Horticulture, 030104 developmental biology, chemistry, 13. Climate action, Yield (chemistry), [SDE]Environmental Sciences, Dormancy, sulphur, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

International audience; High temperatures during the crop reproductive stage impact seed yield and quality. The changing climate will require consideration of the effects of high temperature events that differ from their intensity, their duration and their frequency over the seed quality-building stages. The impact of these features deserve to be investigated at the light of induced thermo-sensitization which can lead to alleviate expected negative impacts. In our work, maturing seeds of the sulphur-demanding crop, oilseed rape, were exposed to several temperature sequences that varied in intensity, duration and frequency at the onset of seed maturation. Results-measured in seeds that were at the onset of maturation when the temperature stress occurred-indicated that (i) the longer the cumulated duration of the temperature stress, the more negatively impacted the quality criteria with decreased fatty acids (FAs) concentration, increased omega 6:omega 3 ratio, lower seed membrane integrity and increased seed dormancy and (ii) a mild stress event prior to heat peaks had an alleviating effect on the negative impact of the later heat peaks (priming effect) on seed nitrogen, desiccation tolerance and the phytohormones involved in thermoinhibition. Sulphur restriction was positive on FAs, protein concentration and negative on breaking dormancy. In addition, sulphur supply interfered with temperature modality, features such that positive impact of sulphur limitation on boosting oxidative response were cancelled with intense late heat peaks. This work provides insights to define thermopriming protocols in relation to the timing of quality building processes, their respective optimal temperature and adequate sulphur supply.

Published
2021

12. High temperature patterns during seed maturation determine seed yield and quality in oilseed rape (Brassica napus L.) in relation to sulfur nutrition

Author

Pierre-Louis Teissedre, Sophie Brunel-Muguet, Annette Morvan-Bertrand, Lethicia Magno Massuia de Almeida, Jean Christophe Avice, Tae-Hwan Kim, María Reyes González-Centeno, Alain Mollier, Jean-Jacques Bessoule, Marina Le Guédard, and Marie Hélène Wagner

Subjects
chemistry.chemical_classification, Brassica, chemistry.chemical_element, Fatty acid, Biology, biology.organism_classification, Sulfur, Desiccation tolerance, Crop, Horticulture, chemistry, Mild stress, Yield (chemistry), Dormancy
Abstract

High temperatures (HTs) during the crop reproductive stage impact seed yield and quality. The changing climate will require consideration of the effects of repeated HT events following evidence for non-additive effects due to beneficial stress memory or amplification of individual event effects. Maturing seeds of the sulfur (S)-demanding crop, oilseed rape, were exposed to several HT sequences that varied in intensity, duration and frequency at the onset of seed maturation. The effects of these sequences in combination with two contrasting S supplies are reported. The results indicated that (i) as the number of HT days in a sequence increased, fatty acid (FA) concentrations decreased, the proportion of unsaturated FAs decreased, seed membranes were damaged, desiccation tolerance was lost, and dormancy increased, regardless of event intensity and (ii) a mild stress event prior to heat peaks had a priming effect on desiccation tolerance and the phytohormones involved in HT-induced thermoinhibition. Low S nutrition amplified or alleviated the effects of the HT sequences due the requirement for S in enhancing seed storage synthesis or inducing stress memory-associated mechanisms. This work provides insights to define thermopriming protocols in relation to the timing of quality building processes and their respective optimal temperature.

Published
2020

13. LPIAT, a lyso-Phosphatidylinositol Acyltransferase, Modulates Seed Germination in Arabidopsis thaliana through PIP Signalling Pathways and is Involved in Hyperosmotic Response

Author

Magali S. Grison, Jean-Jacques Bessoule, Stéphanie Pascal, Lionel Faure, Valerie Wattelet-Boyer, Denis Coulon, Jonathan Clark, Eric Testet, Marina Le Guédard, Laboratoire de biogenèse membranaire (LBM), and Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, Salinity, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, Plant Roots, 01 natural sciences, lcsh:Chemistry, chemistry.chemical_compound, Osmoregulation, Phosphatidylinositol Phosphates, acyltransferase, Arabidopsis thaliana, Abscisic acid, lcsh:QH301-705.5, Spectroscopy, biology, food and beverages, General Medicine, phosphoinositides, Hypocotyl, Computer Science Applications, Cell biology, Phenotype, Germination, Acyltransferase, Seeds, lipids (amino acids, peptides, and proteins), Signal Transduction, aleurone-like cells, seed germination, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, aba, Phosphatidylinositol, Physical and Theoretical Chemistry, Molecular Biology, Arabidopsis Proteins, Organic Chemistry, Lipid metabolism, biology.organism_classification, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, hyperosmotic stress, Mutation, Acyl Coenzyme A, Hyperosmotic response, Acyltransferases, Abscisic Acid, 010606 plant biology & botany
Abstract

Lyso-lipid acyltransferases are enzymes involved in various processes such as lipid synthesis and remodelling. Here, we characterized the activity of an acyltransferase from Arabidopsis thaliana (LPIAT). In vitro, this protein, expressed in Escherichia coli membrane, displayed a 2-lyso-phosphatidylinositol acyltransferase activity with a specificity towards saturated long chain acyl CoAs (C16:0- and C18:0-CoAs), allowing the remodelling of phosphatidylinositol. In planta, LPIAT gene was expressed in mature seeds and very transiently during seed imbibition, mostly in aleurone-like layer cells. Whereas the disruption of this gene did not alter the lipid composition of seed, its overexpression in leaves promoted a strong increase in the phosphatidylinositol phosphates (PIP) level without affecting the PIP2 content. The spatial and temporal narrow expression of this gene as well as the modification of PIP metabolism led us to investigate its role in the control of seed germination. Seeds from the lpiat mutant germinated faster and were less sensitive to abscisic acid (ABA) than wild-type or overexpressing lines. We also showed that the protective effect of ABA on young seedlings against dryness was reduced for lpiat line. In addition, germination of lpiat mutant seeds was more sensitive to hyperosmotic stress. All these results suggest a link between phosphoinositides and ABA signalling in the control of seed germination

Published
2020

14. Impact of diuron and S-metolachlor on the freshwater diatom Gomphonema gracile: Complementarity between fatty acid profiles and different kinds of ecotoxicological impact-endpoints

Author

Agnès Feurtet-Mazel, Imane Elfeky, Patrice Gonzalez, Soizic Morin, Laura Malbezin, Mélissa Eon, Nicolas Mazzella, Jean-Jacques Bessoule, Floriane Demailly, François Delmas, and Marina Le Guédard

Subjects
Chlorophyll a, Environmental Engineering, 010504 meteorology & atmospheric sciences, 010501 environmental sciences, Photosynthesis, 01 natural sciences, chemistry.chemical_compound, Algae, Acetamides, Toxicity Tests, Environmental Chemistry, Food science, Waste Management and Disposal, Carotenoid, 0105 earth and related environmental sciences, chemistry.chemical_classification, Diatoms, biology, Herbicides, Fatty Acids, Fatty acid, Pesticide, biology.organism_classification, Pollution, Diatom, chemistry, Diuron, Water Pollutants, Chemical, Polyunsaturated fatty acid
Abstract

Fatty acids (FA) are crucial for the maintenance of membrane fluidity and play a central role in metabolic energy storage. Polyunsaturated fatty acids play an essential ecological role since they are key parameters in the nutritional value of algae. Pesticide impacts on fatty acid profiles have been documented in marine microalgae, but remain understudied in freshwater diatoms. The aims of this study were to: 1) investigate the impact of diuron and S-metolachlor on “classical descriptors” (photosynthesis, growth rate, pigment contents, and on the expression levels of target genes in freshwater diatoms), 2) examine the impact of these pesticides on diatom fatty acid profiles and finally, 3) compare fatty acid profiles and “classical descriptor” responses in order to evaluate their complementarity and ecological role. To address this issue, the model freshwater diatom Gomphonema gracile was exposed during seven days to diuron and S-metolachlor at 10 μg.L−1. G. gracile was mostly composed of the following fatty acids: 20:5n3; 16:1; 16:0; 16:3n4; 14:0 and 20:4n6 and highly unsaturated fatty acids were overall the best represented fatty acid class. S-metolachlor decreased the growth rate and chlorophyll a content of G. gracile and induced the expression of cox1, nad5, d1 and cat genes, while no significant impacts were observed on photosynthesis and carotenoid content. In a more global way, S-metolachlor did not impact the fatty acid profiles of G. gracile. Diuron inhibited photosynthesis, growth rate, chlorophyll a content and induced cat and d1 gene expressions but no significant effect was observed on carotenoid content. Diuron decreased the percentage of highly unsaturated fatty acids but increased the percentage of monounsaturated fatty acids. These results demonstrated that fatty acids responded to diuron conversely to pigment content, suggesting that fatty acids can inform on energy content variation in diatoms subjected to herbicide stress.

Published
2019

15. A new approach to the analysis and evaluation of the research output of countries and institutions

Author

Jean-Jacques Bessoule, Domingo Docampo, University of Vigo [ Pontevedra], Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB), and Bessoule, Jean-jacques

Subjects
research output, Computer science, media_common.quotation_subject, [SDV]Life Sciences [q-bio], bibliometric indicators, Library and Information Sciences, 050905 science studies, [SHS.STAT] Humanities and Social Sciences/Methods and statistics, Institution, countries, institutions, Simplicity, publications, ComputingMilieux_MISCELLANEOUS, media_common, [SHS.STAT]Humanities and Social Sciences/Methods and statistics, 05 social sciences, Scientific production, General Social Sciences, Data science, Computer Science Applications, citations, Tracking (education), 0509 other social sciences, 050904 information & library sciences, Strengths and weaknesses
Abstract

International audience; A plethora of bibliometric indicators is available nowadays to gauge research performance. The spectrum of bibliometric based measures is very broad, from purely size-dependent indicators (e.g. raw counts of scientific contributions and/or citations) up to size-independent measures (e.g. citations per paper, publications or citations per researcher), through a number of indicators that effectively combine quantitative and qualitative features (e.g. the h-index). In this paper we present a straightforward procedure to evaluate the scientific contribution of territories and institutions that combines size-dependent and scale-free measures. We have analysed in the paper the scientific production of 189 countries in the period 2006-2015. Our approach enables effective global and field-related comparative analyses of the scientific productions of countries and academic/research institutions. Furthermore, the procedure helps to identifying strengths and weaknesses of a given country or institution, by tracking variations of performance ratios across research fields. Moreover, by using a straightforward wealth-index, we show how research performance measures are highly associated with the wealth of countries and territories. Given the simplicity of the methods introduced in this paper and the fact that their results are easily understandable by non-specialists, we believe they could become a useful tool for the assessment of the research output of countries and institutions.

Published
2019

16. Trace element bioavailability, yield and seed quality of rapeseed (Brassica napus L.) modulated by biochar incorporation into a contaminated technosol

Author

Alain Ourry, Sophie Brunel-Muguet, Claire Grosbellet, William Galland, Lilian Marchand, Sébastien Mongrand, Anne Maillard, Nadège Oustriere, Céline Pelosi, María Reyes González-Centeno, Jean-Jacques Bessoule, Qinzhong Zhang, Michel Mench, Valérie Bert, Annette Morvan-Bertrand, Pierre-Louis Teissedre, Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Key Laboratory of Agricultural Environment, Ministry of Agriculture, Sino-Australian Joint Laboratory for Sustainable Agro-Ecosystems, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences (CAAS), Institut National de l'Environnement Industriel et des Risques (INERIS), Biodiversité, Gènes et Communautés, Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), USC : Œnologie, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique ( INRA ), UMR 1202 BIOGECO, Université Sciences et Technologies - Bordeaux 1, Ecologie fonctionnelle et écotoxicologie des agroécosystèmes ( ECOSYS ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Université Paris Saclay, Ecophysiologie Végétale, Agronomie et Nutritions ( EVA ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut National de la Recherche Agronomique ( INRA ), Normandie Université ( NU ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bordeaux ( UB ) -Institut des Sciences de la Vigne et du Vin (ISVV), UMR 5200 CNRS-Université Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 950 Ecophysiologie Végétale, Agronomie et nutritions N, C, S, Chinese Academy of Agricultural Sciences ( CAAS ), Technologies and Sustainable and Clean Processes, and Institut National de l'Environnement Industriel et des Risques

Subjects
0106 biological sciences, Environmental Engineering, Rapeseed, phytomanagement, Photochemistry, cadmium, Health, Toxicology and Mutagenesis, [SDE.MCG]Environmental Sciences/Global Changes, Amendment, Biological Availability, chemistry.chemical_element, Zinc, Technosol, 010501 environmental sciences, 01 natural sciences, brassica napus, phytoextraction, Ammonium Compounds, Biochar, Botany, Soil Pollutants, Environmental Chemistry, assainissement du sol, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Biomass, 0105 earth and related environmental sciences, 2. Zero hunger, Cadmium, lead, zinc, Public Health, Environmental and Occupational Health, General Medicine, General Chemistry, Pollution, 6. Clean water, Trace Elements, Manure, Soil conditioner, [ SDE.MCG ] Environmental Sciences/Global Changes, Horticulture, Phytoremediation, Biodegradation, Environmental, chemistry, Metals, Charcoal, copper, Seeds, 010606 plant biology & botany, technosol
Abstract

Background and aims Rapeseed ( Brassica napus L.) is a Cd/Zn-accumulator whereas soil conditioners such as biochars may immobilize trace elements. These potentially complementary soil remediation options were trialed, singly and in combination, in a pot experiment with a metal( loid )-contaminated technosol. Methods The technosol [total content in mg kg −1 Zn 6089, Cd 9.4, Cu 110, and Pb 956] was either amended (2% w/w) or not with a poultry manure-derived biochar. Rapeseed was cultivated for both soil treatments during 24 weeks up to harvest under controlled conditions. Results Biochar incorporation into the technosol promoted the As, Cd, Cu, Mo, Ni, Pb and Zn solubility. It decreased foliar B, Cu and Mo concentrations, and Mo concentration in stems, pericarps and seeds. But, it did not impact neither the biomass of aerial rapeseed parts (except a decrease for seeds), nor their C (except a decrease for stems), seed fatty acid, seed starch and soluble sugar contents, and antioxidant capacity in both leaves and seeds. Biochar amendment increased the phytoextraction by aerial plant parts for K, P, and S, reduced it for N, Ca, B, Mo, Ni and Se, whereas it remained steady for Mg, Zn, Fe, Mn, Cu, Cd and Co. Conclusions The biochar incorporation into this technosol did not promote Cd, Cu and Zn phytoextraction by rapeseed and its potential oilseed production, but increased the solubility of several metal( loid )s. Here Zn and Cd concentrations in the soil pore water were decreased by rapeseed, showing the feasibility to strip available soil Zn and Cd in combination with seed production.

Published
2016

17. Modulation of metal bioavailability for two earthworm species by the use of biochar

Author

Lilian Marchand, Céline Pelosi, Christian Mougin, Gonzalez Centeno, M., Anne Maillard, Alain Ourry, William Galland, Pierre Louis Teissedre, Jean-Jacques Bessoule, Sebastien Mongrand, Annette Morvan-Bertrand, Zhang, Q., Claire Grosbellet, Valérie Bert, Nadège Oustrière, Michel Mench, Sophie Brunel-Muguet, Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université de Bordeaux (UB), Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Chinese Academy of Agricultural Sciences (CAAS), Florentaise, and Institut National de l'Environnement Industriel et des Risques (INERIS)

Subjects
élément trace, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, phytoremédiation, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

18. Lipids of plant membrane rafts

Author

Patrick Moreau, Sébastien Mongrand, Patricia Gerbeau-Pissot, Corinne Buré, Jean-Jacques Bessoule, Fabienne Furt, Françoise Simon-Plas, Jean-Luc Cacas, Marina Le Guédard, and Jean-Marie Schmitter

Subjects
0106 biological sciences, 0303 health sciences, fungi, Lipid microdomain, food and beverages, Membrane raft, Cell Biology, Raft, Plasmodesma, Biology, Lipids, 01 natural sciences, Biochemistry, Sphingolipid, Cell biology, 03 medical and health sciences, Membrane Microdomains, Membrane, Plant Cells, lipids (amino acids, peptides, and proteins), Lipid raft, 030304 developmental biology, 010606 plant biology & botany
Abstract

Lipids tend to organize in mono or bilayer phases in a hydrophilic environment. While they have long been thought to be incapable of coherent lateral segregation, it is now clear that spontaneous assembly of these compounds can confer microdomain organization beyond spontaneous fluidity. Membrane raft microdomains have the ability to influence spatiotemporal organization of protein complexes, thereby allowing regulation of cellular processes. In this review, we aim at summarizing briefly: (i) the history of raft discovery in animals and plants, (ii) the main findings about structural and signalling plant lipids involved in raft segregation, (iii) imaging of plant membrane domains, and their biochemical purification through detergent-insoluble membranes, as well as the existing debate on the topic. We also discuss the potential involvement of rafts in the regulation of plant physiological processes, and further discuss the prospects of future research into plant membrane rafts.

Published
2012

19. Polyphosphoinositides Are Enriched in Plant Membrane Rafts and Form Microdomains in the Plasma Membrane

Author

Elodie Noirot, Sébastien Mongrand, Ingo Heilmann, Jean-Jacques Bessoule, Jeanine Lherminier, Rémi Zallot, Fabienne Furt, Françoise Sargueil, Françoise Simon-Plas, Thomas Stanislas, Sabine König, Université de Bordeaux Ségalen [Bordeaux 2], Georg-August-University [Göttingen], Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), and Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)

Subjects
0106 biological sciences, Physiology, Nicotiana tabacum, Phosphatidylinositol Phosphates, nicotiana tabacum, Plant Science, Plants genetics, membrane plasmique, MICRODOMAINE, 01 natural sciences, DIMs, MICORSCOPIE, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, Cell membrane, 03 medical and health sciences, chemistry.chemical_compound, Génétique des plantes, Genetics, medicine, Phosphatidylinositol, membrane, lipide, 030304 developmental biology, 0303 health sciences, acide gras, biology, polyphosphoinositide, Metabolism, biology.organism_classification, Sterol, tabac, Membrane, medicine.anatomical_structure, chemistry, Biochemistry, Signal transduction, 010606 plant biology & botany
Abstract

L'article original est publié par The American Society of Plant Biologists; International audience; In this article, we analyzed the lipid composition of detergent-insoluble membranes (DIMs) purified from tobacco (Nicotiana tabacum) plasma membrane (PM), focusing on polyphosphoinositides, lipids known to be involved in various signal transduction events. Polyphosphoinositides were enriched in DIMs compared with whole PM, whereas all structural phospholipids were largely depleted from this fraction. Fatty acid composition analyses suggest that enrichment of polyphosphoinositides in DIMs is accompanied by their association with more saturated fatty acids. Using an immunogold-electron microscopy strategy, we were able to visualize domains of phosphatidylinositol 4,5-bisphosphate in the plane of the PM, with 60% of the epitope found in clusters of approximately 25 nm in diameter and 40% randomly distributed at the surface of the PM. Interestingly, the phosphatidylinositol 4,5-bisphosphate cluster formation was not significantly sensitive to sterol depletion induced by methyl-β-cyclodextrin. Finally, we measured the activities of various enzymes of polyphosphoinositide metabolism in DIMs and PM and showed that these activities are present in the DIM fraction but not enriched. The putative role of plant membrane rafts as signaling membrane domains or membrane-docking platforms is discussed.

Published
2010

20. Expression of Rapeseed Microsomal Lysophosphatidic Acid Acyltransferase Isozymes Enhances Seed Oil Content in Arabidopsis

Author

Michel Delseny, Thomas J. Roscoe, Sylvie Maisonneuve, Reneݩ Lessire, and Jean-Jacques Bessoule

Subjects
biology, Physiology, Mutant, food and beverages, Plant Science, Phosphatidic acid, biology.organism_classification, Isozyme, Complementation, chemistry.chemical_compound, Biochemistry, Biosynthesis, chemistry, Arabidopsis, Genetics, Arabidopsis thaliana, Silique
Abstract

In higher plants, lysophosphatidic acid acyltransferase (LPAAT), located in the cytoplasmic endomembrane compartment, plays an essential role in the synthesis of phosphatidic acid, a key intermediate in the biosynthesis of membrane phospholipids in all tissues and storage lipids in developing seeds. In order to assess the contribution of LPAATs to the synthesis of storage lipids, we have characterized two microsomal LPAAT isozymes, the products of homoeologous genes that are expressed in rapeseed (Brassica napus). DNA sequence homologies, complementation of a bacterial LPAAT-deficient mutant, and enzymatic properties confirmed that each of two cDNAs isolated from a Brassica napus immature embryo library encoded a functional LPAAT possessing the properties of a eukaryotic pathway enzyme. Analyses in planta revealed differences in the expression of the two genes, one of which was detected in all rapeseed tissues and during silique and seed development, whereas the expression of the second gene was restricted predominantly to siliques and developing seeds. Expression of each rapeseed LPAAT isozyme in Arabidopsis (Arabidopsis thaliana) resulted in the production of seeds characterized by a greater lipid content and seed mass. These results support the hypothesis that increasing the expression of glycerolipid acyltransferases in seeds leads to a greater flux of intermediates through the Kennedy pathway and results in enhanced triacylglycerol accumulation.

Published
2009

21. PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast

Author

Denis Coulon, René Lessire, Gisèle Velours, Eric Testet, Jean-Jacques Bessoule, Valérie Boyer, Willem Kulik, Sophie Ayciriex, Marina Le Guédard, Christer S. Ejsing, and Andrej Shevchenko

Subjects
Phospholipid, Cell Biology, Biochemistry, Palmitic acid, chemistry.chemical_compound, chemistry, Stearate, Acyltransferase, Saturated fatty acid, Lysophosphatidylinositol, lipids (amino acids, peptides, and proteins), Phosphatidylinositol, Stearic acid, Molecular Biology
Abstract

In yeast, both phosphatidylinositol and phosphatidylserine are synthesized from cytidine diphosphate-diacylglycerol. Because, as in other eukaryotes, phosphatidylinositol contains more saturated fatty acids than phosphatidylserine (and other phospholipids), it has been hypothesized that either phosphatidylinositol is synthesized from distinct cytidine diphosphate-diacylglycerol molecules, or that, after its synthesis, it is modified by a hypothetical acyltransferase that incorporates saturated fatty acid into neo-synthesized molecules of phosphatidylinositol. We used database search methods to identify an acyltransferase that could catalyze such an activity. Among the various proteins that we studied, we found that Psi1p (phosphatidylinositol stearoyl incorporating 1 protein) is required for the incorporation of stearate into phosphatidylinositol because GC and MS analyses of psi1Δ lipids revealed an almost complete disappearance of stearic (but not of palmitic acid) at the sn-1 position of this phospholipid. Moreover, it was found that, whereas glycerol 3-phosphate, lysophosphatidic acid and 1-acyl lysophosphatidylinositol acyltransferase activities were similar in microsomal membranes isolated from wild-type and psi1Δ cells, microsomal membranes isolated from psi1Δ cells are devoid of the sn-2-acyl-1-lysolysophosphatidylinositol acyltransferase activity that is present in microsomal membranes isolated from wild-type cells. Moreover, after the expression of PSI1 in transgenic psi1Δ cells, the sn-2-acyl-1-lysolysophosphatidylinositol acyltransferase activity was recovered, and was accompanied by a strong increase in the stearic acid content of lysophosphatidylinositol. As previously suggested for phosphatidylinositol from animal cells (which contains almost exclusively stearic acid as the saturated fatty acid), the results obtained in the present study demonstrate that the existence of phosphatidylinositol species containing stearic acid in yeast results from a remodeling of neo-synthesized molecules of phosphatidylinositol.

Published
2009

22. Discovery and Characterization of an Arabidopsis thaliana N-Acylphosphatidylethanolamine Synthase

Author

Jeanny Laroche-Traineau, Jean-Jacques Bessoule, Jean-Marie Schmitter, Eric Testet, Marina Le Guédard, René Lessire, Lionel Faure, and Denis Coulon

Subjects
Nape, Molecular Sequence Data, Arabidopsis, Lipids and Lipoproteins: Metabolism, Regulation, and Signaling, Plant Roots, Biochemistry, Bacterial Proteins, Gene Expression Regulation, Plant, Hydrolase, Escherichia coli, Phospholipase D, medicine, Protein Isoforms, Arabidopsis thaliana, Amino Acid Sequence, Molecular Biology, chemistry.chemical_classification, Sequence Homology, Amino Acid, ATP synthase, biology, Hydrolysis, Cell Biology, Plants, biology.organism_classification, Lipids, Luminescent Proteins, medicine.anatomical_structure, Enzyme, chemistry, Acyltransferase, biology.protein, Chromatography, Thin Layer, Heterologous expression, Acyltransferases
Abstract

N-Acylethanolamines (NAEs) are lipids involved in several physiological processes in animal and plant cells. In brain, NAEs are ligands of endocannabinoid receptors, which modulate various signaling pathways. In plant, NAEs regulate seed germination and root development, and they are involved in plant defense against pathogen attack. This signaling activity is started by an enzyme called N-acylphosphatidylethanolamine (NAPE) synthase. This catalyzes the N-acylation of phosphatidylethanolamine to form NAPE, which is most likely hydrolyzed by phospholipase D beta/gamma isoforms to generate NAE. This compound is further catabolized by fatty amide hydrolase. The genes encoding the enzymes involved in NAE metabolism are well characterized except for the NAPE synthase gene(s). By heterologous expression in Escherichia coli and overexpression in plants, we characterized an acyltransferase from Arabidopsis thaliana (At1g78690p) catalyzing the synthesis of lipids identified as NAPEs (two-dimensional TLC, phospholipase D hydrolysis assay, and electrospray ionization-tandem mass spectrometry analyses). The ability of free fatty acid and acyl-CoA to be used as acyl donor was compared in vitro with E. coli membranes and purified enzyme (obtained by immobilized metal ion affinity chromatography). In both cases, NAPE was synthesized only in the presence of acyl-CoA. beta-Glucuronidase promoter experiments revealed a strong expression in roots and young tissues of plants. Using yellow fluorescent protein fusion, we showed that the NAPE synthase is located in the plasmalemma of plant cells.

Published
2009

23. Plant Lipid Rafts, Fluctuat nec mergitur

Author

Fabienne Furt, Sébastien Mongrand, Julie V. Cullimore, Benoit Lefebvre, and Jean-Jacques Bessoule

Subjects
biology, fungi, food and beverages, Lipid metabolism, Plant Science, biology.organism_classification, Sphingolipid, Sterol, Medicago truncatula, Cell biology, Membrane, Biochemistry, Lipid droplet, lipids (amino acids, peptides, and proteins), Signal transduction, Lipid raft
Abstract

Lipid rafts in plasma membranes are hypothesized to play key roles in many cellular processes including signal transduction, membrane trafficking and entry of pathogens. We recently documented the biochemical characterization of lipid rafts, isolated as detergent-insoluble membranes, from Medicago truncatula root plasma membranes. We evidenced that the plant-specific lipid steryl-conjugates are among the main lipids of rafts together with free sterols and sphingolipids. An extensive proteomic analysis showed the presence of a specific set of proteins common to other lipid rafts, plus the presence of a redox system around a cytochrome b(561) not previously identified in lipid rafts of either plants or animals. Here, we discuss the similarities and differences between the lipids and proteins of plant and animal lipid rafts. Moreover we describe the potential biochemical functioning of the M. truncatula root lipid raft redox proteins and question whether they may play a physiological role in legume-symbiont interactions.

Published
2007

24. Requirement of Phosphoinositides Containing Stearic Acid To Control Cell Polarity

Author

Jean-Jacques Bessoule, Laetitia Fouillen, Eric Testet, François Doignon, Patricia Laquel, and Karine Tuphile

Subjects
0301 basic medicine, Cell signaling, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Morphogenesis, Biology, Phosphatidylinositols, Actin cytoskeleton organization, 03 medical and health sciences, chemistry.chemical_compound, Cell polarity, Phosphatidylinositol, Molecular Biology, Actin, Cell Polarity, Cell Biology, Articles, biology.organism_classification, Actins, Cell biology, 030104 developmental biology, Biochemistry, chemistry, lipids (amino acids, peptides, and proteins), Intracellular, Acyltransferases, Gene Deletion, Stearic Acids
Abstract

Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of a stearoyl chain at the sn-1 position of phosphatidylinositol. The absence of PIPs containing stearic acid induced disturbances in intracellular trafficking, although the total amount of PIPs was not diminished. Changes in PIPs also induced alterations in the budding pattern and defects in actin cytoskeleton organization (cables and patches). Moreover, when the PSI1 gene was impaired, a high proportion of cells with bipolar cortical actin patches that occurred concomitantly with the bipolar localization of Cdc42p was specifically found among diploid cells. This bipolar cortical actin phenotype, never previously described, was also detected in a bud9Δ/bud9Δ strain. Very interestingly, overexpression of PSI1 reversed this phenotype.

Published
2015

25. Soil bioindicators: how to select them in function of the site issues?

Author

Benjamin Pauget, Laurence Rougé, Antonio Bispo, Cécile Grand, Thierry Béguiristain, Jean-Jacques Bessoule, Josselin Bodilis, Rémi Chaussod, Nathalie Cheviron, Michael Coeurdassier, Jérôme Cortet, Steven Criquet, Samuel Dequiedt, Olivier Faure, Christophe Gangneux, Isabelle Gattin, Marina Le Guedard, Adnane Hitmi, Laurent, N., Marc Legras, Sylvie Nelieu, Ruiz, N., Salima Taïbi, Franck Vandenbulcke, Annette de Vaufleury, Cecile Villenave, Guenola Peres, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Ingénierie Durable, Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Morphodynamique Continentale et Côtière (M2C), Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire Sols et Environnement (LSE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Ecole Nationale Supérieure des Mines (ENSM), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), LEB Aquitaine Transfert-ADERA, Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant - Clermont Auvergne (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Institut de Recherche pour le Développement (IRD Indonésie), Institut de Recherche pour le Développement (IRD), Laboratoire Génie Civil et géoEnvironnement LGCgE-Lille 1, Ecologie Numérique et Ecotoxicologie, Université Lille Nord de France (COMUE), Sol Agro et hydrosystème Spatialisation (SAS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Université de Rennes (UNIV-RENNES), ProdInra, Archive Ouverte, Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Equipe Ecologie Numérique et Ecotoxicologie, Université de Lille, Sciences et Technologies, Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Université de Rennes (UR), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), UMR CNRS 'Ecobio' 6553, Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ), Agence de l'Environnement et de la Maîtrise de l'Energie ( ADEME ), Laboratoire de Biogenèse Membranaire, UMR 5200, Centre National de la Recherche Scientifique ( CNRS ), Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes ( ECOSYS ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Laboratoire Chrono-environnement, UMR UFC/CNRS 6249, Université de Franche-Comté ( UFC ), Laboratoire Sols et Environnement ( LSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Centre d’Ecologie Fonctionnelle et Evolutive ( CEFE ), Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Montpellier ( UM ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -École pratique des hautes études ( EPHE ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Université Paul-Valéry - Montpellier 3 ( UM3 ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Aix Marseille Université ( AMU ) -Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique ( CNRS ), Ecole Nationale Supérieure des Mines ( ENSM ), ESITPA, Laboratoire de Biogénèse Membranaire UMR 5200, Université de Bordeaux Ségalen (Bordeaux 2), LEB Aquitaine Transfert – ADERA, Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant - Clermont Auvergne ( PIAF ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Ecole Nationale d'Ingénieurs des Techniques Agricoles, Institut de Recherche pour le Développement ( IRD Indonésie ), Institut de Recherche pour le Développement ( IRD ), Université Lille Nord de France, Sol Agro et hydrosystème Spatialisation ( SAS ), Institut National de la Recherche Agronomique ( INRA ) -AGROCAMPUS OUEST, and Université de Rennes ( UNIV-RENNES )

Subjects
[SDV] Life Sciences [q-bio], [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

Soil bioindicators: how to select them in function of the site issues?. 13th International AquaConSoil Conference

Published
2015

26. Complementation of Seed Maturation Phenotypes by Ectopic Expression of ABSCISIC ACID INSENSITIVE3, FUSCA3 and LEAFY COTYLEDON2 in Arabidopsis

Author

Thomas T. Roscoe, Jocelyne Guilleminot, Martine Devic, Frédéric Berger, Jean-Jacques Bessoule, Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biogenèse membranaire (LBM), and Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Physiology, Triacylglycerol, B3 domain, [SDV]Life Sciences [q-bio], Arabidopsis, Plant Science, Biology, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Storage protein, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Gene Regulatory Networks, Seed development, Leafy, Abscisic acid, 030304 developmental biology, Genetics, chemistry.chemical_classification, 0303 health sciences, Arabidopsis Proteins, Fatty Acids, Genetic Complementation Test, Embryo, Starch, Cell Biology, General Medicine, Lipid Metabolism, Plants, Genetically Modified, biology.organism_classification, Complementation, Phenotype, chemistry, Mutation, Seeds, Fus3, Ectopic expression, Biomarkers, Transcription Factors, 010606 plant biology & botany
Abstract

International audience; ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3) and LEAFY COTYLEDON2 (LEC2), collectively the AFL, are master regulators of seed maturation processes. This study examined the role of AFL in the production of seed reserves in Arabidopsis. Quantification of seed reserves and cytological observations of afl mutant embryos show that protein and lipid but not starch reserves are spatially regulated by AFL. Although AFL contribute to a common regulation of reserves, ABI3 exerts a quantitatively greater control over storage protein content whereas FUS3 controls lipid content to a greater extent. Although ABI3 controls the reserve content throughout the embryo, LEC2 and FUS3 regulate reserves in distinct embryonic territories. By analyzing the ability of an individual ectopically expressed AFL to suppress afl phenotypes genetically, we show that conserved domains common to each component of the AFL are sufficient for the initiation of storage product synthesis and the establishment of embryo morphology. This confirms redundancy among the AFL and indicates a threshold necessary for function within the AFL pool. Since no individual AFL was able to suppress the tolerance to desiccation, mid- and late-maturation programs were uncoupled.

Published
2015

27. Soil bioindicators: how soil properties influence their responses and to select them in function of the site issues?

Author

Benjamin Pauget, Laurence Rougé, Antonio Bispo, Cécile Grand, Thierry Béguiristain, Jean-Jacques Bessoule, Josselin Bodilis, Rémi Chaussod, Nathalie Cheviron, Michael Coeurdassier, Jérôme Cortet, Steven Criquet, Samuel Dequiedt, Olivier Faure, Christophe Gangneux, Isabelle Gattin, Marina Le Guedard, Adnane Hitmi, Laurent, N., Marc Legras, Sylvie Nelieu, Ruiz, N., Salima Taïbi, Franck Vandenbulcke, Annette De Vaufleury, Cécile Villenave, Guenola Peres, Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Station Biologique de Paimpont CNRS UMR 6653 (OSUR), Université de Rennes (UR), Ingénierie Durable, Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Laboratoire de biogenèse membranaire (LBM), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Morphodynamique Continentale et Côtière (M2C), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Institut National de la Recherche Agronomique (INRA), Laboratoire Sols et Environnement (LSE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), LEB Aquitaine Transfert-ADERA, Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut de Recherche pour le Développement (IRD [France-Nord]), Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 (LGCgE), Université d'Artois (UA)-Université de Lille-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Sol Agro et hydrosystème Spatialisation (SAS), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), UR 0251 Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés, Institut National de la Recherche Agronomique (INRA)-Santé des plantes et environnement (S.P.E.)-Environnement et Agronomie (E.A.)-Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés (PESSAC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Laboratoire Génie Civil et géo-Environnement (LGCgE) - EA 4515 (LGCgE), IMT Lille Douai, Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Lille-Université d'Artois (UA), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Station Biologique de Paimpont CNRS UMR 6653 ( OSUR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ), Ingénierie Durable, Rennes, France., ADEME, Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France., Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Santé des plantes et environnement ( S.P.E. ) -Environnement et Agronomie ( E.A. ) -Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés ( PESSAC ), Laboratoire Sols et Environnement ( LSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Centre d’Ecologie Fonctionnelle et Evolutive ( CEFE ), Université Paul-Valéry - Montpellier 3 ( UM3 ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -École pratique des hautes études ( EPHE ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Aix Marseille Université ( AMU ) -Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique ( CNRS ), Esitpa, Mont Saint Aignan, France., LEB Aquitaine Transfert – ADERA, Villenave d'Ornon, France., Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier ( PIAF ), Institut National de la Recherche Agronomique ( INRA ) -Université Blaise Pascal - Clermont-Ferrand 2 ( UBP ), ESITPA, Ecole Nationale d'Ingénieurs des Techniques Agricoles, Mont Saint Aignan, France., Institut de Recherche pour le Développement ( IRD [France-Nord] ), Université Lille Nord de France - Laboratoire Génie Civil et géoEnvironnement LGCgE-Lille 1, Ecologie Numérique et Ecotoxicologie, Villeneuve d'Ascq, France., Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes ( Eco&Sols ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Institut de Recherche pour le Développement ( IRD ) -Institut National de la Recherche Agronomique ( INRA ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Sol Agro et hydrosystème Spatialisation ( SAS ), Institut National de la Recherche Agronomique ( INRA ) -AGROCAMPUS OUEST, Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université d'Artois (UA)-Université de Lille-IMT Lille Douai, and Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)

Subjects
[ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

28. Involvement of the caleosin/peroxygenase RD20 in the control of cell death during Arabidopsis responses to pathogens

Author

Elizabeth Blée, Thierry Heitz, Jean-Jacques Bessoule, Abdulsamie Hanano, Atomic Energy Commission of Syria, Institut National de la Recherche Agronomique (INRA), Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
0106 biological sciences, Programmed cell death, RD20, oxylipins, Short Communication, [SDV]Life Sciences [q-bio], Arabidopsis, Pseudomonas syringae, Plant Science, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, medicine.disease_cause, 01 natural sciences, Microbiology, 03 medical and health sciences, Gene Expression Regulation, Plant, Lipid droplet, medicine, Homeostasis, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Plant Diseases, Alternaria brassicicola, chemistry.chemical_classification, reactive oxygen species, 0303 health sciences, Reactive oxygen species, biology, Arabidopsis Proteins, Calcium-Binding Proteins, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Biotic stress, biology.organism_classification, Lipid Metabolism, Plants, Genetically Modified, pseudomonas, defense, cell death, chemistry, caleosin, alternaria, Oxidative stress, 010606 plant biology & botany
Abstract

International audience; Caleosins, mostly found in lipid droplets of seeds and leaves, are believed to play physiological roles through their enzymatic capacities to produce oxylipins. We recently identified the caleosin RD20 as a peroxygenase reducing endogenous fatty acid hydroperoxides into their corresponding alcohols. Such oxylipins confer tolerance to oxidative stress by decreasing reactive oxygen species accumulation and by minimizing cell death. RD20 expression being induced by pathogens, we have examined the mode of action of this caleosin in response to biotic stress. Plants overexpressing RD20 exhibited an alteration of their leaf cuticle wax components and an increased resistance to the fungus Alternaria brassicicola. Conversely, silencing RD20 led to an enhanced propagation of the fungus and to reduced severity of the damages caused by the inoculation of the bacteria Pseudomonas syringae pv tomato. We discuss these findings and propose that the major function of RD20 is to generate oxylipins modulating oxidative status and cell death.

Published
2015

29. Which bioindicators are suitable for soil quality monitoring and risk assessment ? From relevance study to transfer tool development

Author

Guenola Peres, Benjamin Pauget, Annette De Vaufleury, Michael Coeurdassier, Marina Leguedard, Jean-Jacques Bessoule, Samuel Dequiedt, Rémi Chaussod, Lionel Ranjard, Daniel Cluzeau, Muriel Guernion, Laurence Rougé, Mickael Hedde, Nathalie Cheviron, C Thoisy-Dur, J., Sylvie Nelieu, Christian Mougin, Isabelle Gattin, Christophe Gangneux, Laurent, N., Marc Legras, Karine Laval, Patrice Lepelletier, Salima Taïbi, Cecile Villenave, Olivier Faure, Jennifer Hellal, Jérôme Cortet, Thierry Beguiristain, Corinne Leyval, Josselin Bodilis, Steven Criquet, Adnane Hitmi, Nuria Ruiz, Franck Vandenbulcke, Cécile Grand, Laurence Galsomies, Antonio Bispo, Sol Agro et hydrosystème Spatialisation ( SAS ), Institut National de la Recherche Agronomique ( INRA ) -AGROCAMPUS OUEST, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), LEB Aquitaine Transfert – ADERA, Villenave d'Ornon, France., Agroécologie [Dijon], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Station Biologique de Paimpont CNRS UMR 6653 ( OSUR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ), Ingénierie Durable, Rennes, France., UR 0251 Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés, Institut National de la Recherche Agronomique ( INRA ) -Santé des plantes et environnement ( S.P.E. ) -Environnement et Agronomie ( E.A. ) -Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés ( PESSAC ), Esitpa, Mont Saint Aignan, France., ESITPA, Ecole Nationale d'Ingénieurs des Techniques Agricoles, Mont Saint Aignan, France., Unite AgriTerr, Mont St Aignan, France., ESITPA, Ecole Superieure des Ingenieurs et Techniciens pour l'Agriculture, Mont Saint Aignan, France., Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes ( Eco&Sols ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Institut de Recherche pour le Développement ( IRD ) -Institut National de la Recherche Agronomique ( INRA ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), École des Mines de Saint-Étienne ( Mines Saint-Étienne MSE ), Institut Mines-Télécom [Paris], Bureau de Recherches Géologiques et Minières (BRGM) ( BRGM ), Laboratoire Sols et Environnement ( LSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Centre d’Ecologie Fonctionnelle et Evolutive ( CEFE ), Université Paul-Valéry - Montpellier 3 ( UM3 ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -École pratique des hautes études ( EPHE ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Interactions Arbres-Microorganismes ( IAM ), Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Aix Marseille Université ( AMU ) -Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique ( CNRS ), IUT de Clermont Ferrand, Institut Universitaire de Technologie de Clermont­ Ferrand - Laboratoire de PBV, Aurillac, France., Institut de Recherche pour le Développement ( IRD [France-Nord] ), USTL, Université des Sciences et Technologies (Lille 1) - Département Génie Biologique, Villeneuve d’Ascq, France., ADEME, Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France., ADEME, Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France, Sol Agro et hydrosystème Spatialisation (SAS), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), LEB Aquitaine Transfert-ADERA, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Station Biologique de Paimpont CNRS UMR 6653 (OSUR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université Paris Diderot - Paris 7 (UPD7), Institut National de la Recherche Agronomique (INRA)-Santé des plantes et environnement (S.P.E.)-Environnement et Agronomie (E.A.)-Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés (PESSAC), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Laboratoire Sols et Environnement (LSE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Morphodynamique Continentale et Côtière (M2C), Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD [France-Nord]), Département Génie Biologique, Université de Lille, Sciences et Technologies, Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR), Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Institut National de la Recherche Agronomique (INRA), Plateforme BIOCHEM-ENV, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud])

Subjects
[ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Introduction- Considering the few number of bioindicators available for soil risk assessment and soil characterization, a national research programme has been set up in France by the French Agency For Environment and Energy Management (ADEME) to develop indicators able to judge about soil degradation and/or remediation of its properties and functions. Objectives of this “BioIndicator programme” are to (i) develop and validate methods for measuring soil biodiversity and soil functions, (ii) test the sensibility and complementarity of the bioindicators for the characterization of different perturbations (contamination, modification of soil uses), iii) identify relevant bioindicators or endpoints for different purposes (ecological risk assessment, monitoring of soil quality..), iv) transfer the results to stakeholders (policy makers, farmers, research board) by providing suitable tools. Methods- 22 research teams are involved. 47 bioindicators are tested (microorganisms, fauna, flora) in a large panel of situations (47 situations including agricultural, industrial and forest sites) and sampled at the same moment by applying standardised sampling protocols. A common database allows the management of the high number of data (200.000). Results- Bioindicator programme initiates a first national benchmark i.e. baselines values for the different biological groups. It provides relevant tools (battery of bioindicators, biological index) adapted to different environmental targets such as i) evaluation of the impact of agricultural practices (crop rotations, reduced tillage, organic and pesticides management), ii) evaluation of the bioavailability of industrial contaminants, iii) evaluation of the biological state of contaminated soils in order to orientate their future use, iv) soil monitoring of biological state at large scale. Moreover, it provides communication tools, such as bioindicator technical sheets and web-interface addressed to stakeholders (http://ecobiosoil.univ-rennes1.fr/ADEME-Bioindicateur/). Conclusion- this10 years research programme, unique at the European Union scale, permits to improve the knowledge on soil biodiversity and its functioning, and provides tools to stakeholders for future soil management.

Published
2014

30. The upstream oxylipin profile ofArabidopsis thaliana: a tool to scan for oxidative stresses

Author

Alexandra Vial, Jean-Pierre Agnel, Lionel Garnier, Urszula Maciejewska, Lidia Polkowska-Kowalczyk, Marie-Hélène Montané, Jean-Jacques Bessoule, Thierry Douki, Jean-Luc Cacas, Christian Triantaphylidès, and Jean-Luc Montillet

Subjects
Light, Lipoxygenase, Arabidopsis, Plant Science, Oxidative phosphorylation, Environment, medicine.disease_cause, Mass Spectrometry, Lipid peroxidation, chemistry.chemical_compound, Tobacco, Genetics, medicine, Solanum nigrum, chemistry.chemical_classification, Reactive oxygen species, biology, food and beverages, Cell Biology, Carbon Dioxide, Oxylipin, biology.organism_classification, Plant Leaves, Oxidative Stress, Biochemistry, chemistry, Fatty Acids, Unsaturated, biology.protein, lipids (amino acids, peptides, and proteins), Lipid Peroxidation, Reactive Oxygen Species, Oxidative stress, Cadmium, Polyunsaturated fatty acid
Abstract

Various physiological imbalances lead to reactive oxygen species (ROS) overproduction and/or increases in lipoxygenase (LOX) activities, both events ending in lipid peroxidation of polyunsaturated fatty acids (PUFAs). Besides the quantification of such a process, the development of tools is necessary in order to allow the identification of the primary cause of its development and localization. A biochemical method assessing 9 LOX, 13 LOX and ROS-mediated peroxidation of membrane-bound and free PUFAs has been improved. The assay is based on the analysis of hydroxy fatty acids derived from PUFA hydroperoxides by both the straight and chiral phase high-performance liquid chromatography. Besides the upstream products of peroxidation of the 18:2 and 18:3 PUFAs, products coming from the 16:3 were characterized and their steady-state level quantified. Moreover, the observation that the relative amounts of the ROS-mediated peroxidation isomers of 18:3 were constant in leaves allowed us to circumvent the chiral analyses for the discrimination and quantification of 9 LOX, 13 LOX and ROS-mediated processes in routine experiments. The methodology has been successfully applied to decipher lipid peroxidation in Arabidopsis leaves submitted to biotic and abiotic stresses. We provide evidence of the relative timing of enzymatic and non-enzymatic lipid peroxidation processes. The 13 LOX pathway is activated early whatever the nature of the stress, leading to the peroxidation of chloroplast lipids. Under cadmium stress, the 9 LOX pathway added to the 13 LOX one. ROS-mediated peroxidation was mainly driven by light and always appeared as a late process.

Published
2004

31. Lipid Rafts in Higher Plant Cells

Author

Françoise Simon-Plas, Marc Bonneu, Johanne Morel, Sébastien Mongrand, Stéphane Claverol, Jean-Pierre Carde, Marie-Andrée Hartmann, Jean-Jacques Bessoule, René Lessire, and Jeanny Laroche

Subjects
Gel electrophoresis, Ergosterol, NADPH oxidase, biology, Cell Biology, medicine.disease_cause, Biochemistry, Sphingolipid, Cell biology, chemistry.chemical_compound, Membrane, chemistry, Membrane protein, Protein targeting, medicine, biology.protein, lipids (amino acids, peptides, and proteins), Molecular Biology, Lipid raft
Abstract

A large body of evidence from the past decade supports the existence of functional microdomains in membranes of animal and yeast cells, which play important roles in protein sorting, signal transduction, or infection by pathogens. They are based on the dynamic clustering of sphingolipids and cholesterol or ergosterol and are characterized by their insolubility, at low temperature, in nonionic detergents. Here we show that similar microdomains also exist in plant plasma membrane isolated from both tobacco leaves and BY2 cells. Tobacco lipid rafts were found to be greatly enriched in a sphingolipid, identified as glycosylceramide, as well as in a mixture of stigmasterol, sitosterol, 24-methylcholesterol, and cholesterol. Phospho- and glycoglycerolipids of the plasma membrane were largely excluded from lipid rafts. Membrane proteins were separated by one- and two-dimensional gel electrophoresis and identified by tandem mass spectrometry or use of specific antibody. The data clearly indicate that tobacco microdomains are able to recruit a specific set of the plasma membrane proteins and exclude others. We demonstrate the recruitment of the NADPH oxidase after elicitation by cryptogein and the presence of the small G protein NtRac5, a negative regulator of NADPH oxidase, in lipid rafts.

Published
2004

32. The product of the UTH1 gene, required for Bax-induced cell death in yeast, is involved in the response to rapamycin

Author

Angela Grelaud-Coq, Muriel Priault, Stéphen Manon, Jean-Jacques Bessoule, Nadine Camougrand, and Esther Marza

Subjects
Programmed cell death, Bcl-2-associated X protein, Apoptosis, Autophagy, Mutant, biology.protein, Biology, Mitochondrion, Bacterial outer membrane, Molecular Biology, Microbiology, Mitochondrial apoptosis-induced channel, Cell biology
Abstract

A yeast mutant was isolated that was resistant to Bax-induced cell death. It supports a mutation leading to decreased amounts of the protein Uth1p. A strain in which the UTH1 gene is disrupted also exhibits resistance to Bax expression. The absence of Uth1p does not change the mitochondrial localization of Bax, its insertion in the mitochondrial outer membrane or its cytochrome c-releasing activity. On the other hand, the absence of Uth1p does prevent the appearance of other hallmarks related to Bax expression in yeast, such as oxidation of mitochondrial lipid, production of reactive oxygen species and maintenance of plasma membrane properties after ethanol stress. The absence of Uth1p was also found to induce resistance to rapamycin, a specific inducer of autophagy. This resistance only appears when cells are grown under respiratory conditions, but not under fermentative conditions, suggesting that Uth1p acts in an autophagic pathway involving mitochondria, in accordance with its main localization in the outer mitochondrial membrane. Taken together, these data show that Bax is able to activate a death pathway related to autophagy in yeast, which also exhibits typical hallmarks of apoptosis, revealing a possible dual function of Bax in both types of death. This hypothesis is discussed in the light of observations suggesting a co-regulation of apoptosis and autophagy in mammalian cells.

Published
2003

33. Bax-induced cell death in yeast depends on mitochondrial lipid oxidation

Author

Angela Grelaud-Coq, Nadine Camougrand, Jean-Jacques Bessoule, Muriel Priault, and Stéphen Manon

Subjects
chemistry.chemical_classification, Reactive oxygen species, Programmed cell death, biology, Superoxide, Lipid metabolism, Mitochondrion, Biochemistry, Cell biology, Superoxide dismutase, chemistry.chemical_compound, Bcl-2-associated X protein, chemistry, Lipid oxidation, biology.protein
Abstract

The oxidant function of pro-apoptotic protein Bax was investigated through heterologous expression in yeast. Direct measurements of fatty acid content show that Bax-expression induces oxidation of mitochondrial lipids. This effect is prevented by the coexpression of Bcl-xL. The oxidation actually could be followed on isolated mitochondria as respiration-induced peroxidation of polyunsaturated cis-parinaric acid and on whole cells as the increase in the amount of thiobarbituric acid-reactive products. Treatments that increase the unsaturation ratio of lipids, making them more sensitive to oxidation, increase kinetics of Bax-induced death. Conversely, inhibitors of lipid oxidation and treatments that decrease the unsaturation ratio of fatty acids decrease kinetics of Bax-induced death. Taken together, these results show that Bax-induced mitochondrial lipid oxidation is relevant to Bax-induced cell death. Conversely, lipid oxidation is poorly related to the massive Bax-induced superoxide and hydrogen peroxide accumulation, which occurs at the same time, as chemical or enzymatic scavenging of ROS does not prevent lipid oxidation nor has any effects on kinetics of Bax-induced cell death. Whatever the origin of mitochondrial lipid oxidation, these data show that it represents a major step in the cascade of events leading to Bax-induced cell death. These results are discussed in the light of the role of lipid oxidation both in mammalian apoptosis and in other forms of cell death in other organisms.

Published
2002

34. Les indicateurs de l'état biologique des sols : Guide de sélection selon le type d’application et/ou la problématique

Author

Benjamin Pauget, Laurence Rougé, Guenola Peres, Antonio Bispo, Cécile Grand, Thierry Beguiristain, Jean-Jacques Bessoule, Josselin Bodilis, Rémi Chaussod, Nathalie Cheviron, Michael Coeurdassier, Jérôme Cortet, Stéven Criquet, Samuel Dequiedt, Olivier Faure, Christophe Gangneux, Isabelle Gattin, Marina Le Guédard, Adnane Hitmi, Laurent, N., Marc Legras, Sylvie Nelieu, Ruiz, N., Franck Vandenbulcke, Cécile Villenave, Annette De Vaufleury, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Station Biologique de Paimpont CNRS UMR 6653 (OSUR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Ingénierie Durable, Rennes, France., Université Paris Diderot - Paris 7 (UPD7), Sol Agro et hydrosystème Spatialisation (SAS), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), LEB Aquitaine Transfert-ADERA, Morphodynamique Continentale et Côtière (M2C), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, UR 0251 Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés, Institut National de la Recherche Agronomique (INRA)-Santé des plantes et environnement (S.P.E.)-Environnement et Agronomie (E.A.)-Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés (PESSAC), Laboratoire Sols et Environnement (LSE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut de Recherche pour le Développement (IRD [France-Nord]), Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 (LGCgE), Université d'Artois (UA)-Université de Lille-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-JUNIA (JUNIA), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Laboratoire Génie Civil et géo-Environnement (LGCgE) - EA 4515 (LGCgE), IMT Lille Douai, Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Lille-Université d'Artois (UA), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Laboratoire Chrono-environnement (UMR 6249) (LCE), Université de Rennes (UR), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Institut National de la Recherche Agronomique (INRA), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université d'Artois (UA)-Université de Lille-IMT Lille Douai, Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Station Biologique de Paimpont CNRS UMR 6653 ( OSUR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ), Sol Agro et hydrosystème Spatialisation ( SAS ), Institut National de la Recherche Agronomique ( INRA ) -AGROCAMPUS OUEST, ADEME, Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France., Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), LEB Aquitaine Transfert – ADERA, Villenave d'Ornon, France, Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Santé des plantes et environnement ( S.P.E. ) -Environnement et Agronomie ( E.A. ) -Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés ( PESSAC ), Laboratoire Sols et Environnement ( LSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Centre d’Ecologie Fonctionnelle et Evolutive ( CEFE ), Université Paul-Valéry - Montpellier 3 ( UM3 ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -École pratique des hautes études ( EPHE ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Aix Marseille Université ( AMU ) -Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique ( CNRS ), École des Mines de Saint-Étienne ( Mines Saint-Étienne MSE ), Institut Mines-Télécom [Paris], Esitpa, Mont Saint Aignan, France., LEB Aquitaine Transfert – ADERA, Villenave d'Ornon, France., Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier ( PIAF ), Institut National de la Recherche Agronomique ( INRA ) -Université Blaise Pascal - Clermont-Ferrand 2 ( UBP ), ESITPA, Ecole Nationale d'Ingénieurs des Techniques Agricoles, Mont Saint Aignan, France., Institut de Recherche pour le Développement ( IRD [France-Nord] ), Université Lille Nord de France - Laboratoire Génie Civil et géoEnvironnement LGCgE-Lille 1, Ecologie Numérique et Ecotoxicologie, Villeneuve d'Ascq, France., Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes ( Eco&Sols ), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Institut de Recherche pour le Développement ( IRD ) -Institut National de la Recherche Agronomique ( INRA ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro )

Subjects
[SDV] Life Sciences [q-bio], [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

35. The reductase activity of the Arabidopsis caleosin RESPONSIVE TO DESSICATION20 mediates gibberellin-dependent flowering time, abscisic acid sensitivity, and tolerance to oxidative stress

Author

Elizabeth Blée, Jürgen Ehlting, Benoît Boachon, Michel Burcklen, Jean-Jacques Bessoule, Dimitri Heintz, Marina Le Guédard, Abdulsamie Hanano, Ivo Feussner, Cornelia Herrfurth, Université de Strasbourg (UNISTRA), Institut National de la Recherche Agronomique (INRA), Georg-August-University [Göttingen], Centre National de la Recherche Scientifique, French Ministry for Research, Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), and Department for Plant Biochemistry

Subjects
0106 biological sciences, Physiology, [SDV]Life Sciences [q-bio], Arabidopsis, Plant Development, Germination, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Plant Science, Flowers, 01 natural sciences, Substrate Specificity, 03 medical and health sciences, chemistry.chemical_compound, Lipid oxidation, Auxin, Genetics, Arabidopsis thaliana, Abscisic acid, ComputingMilieux_MISCELLANEOUS, Unsaturated fatty acid, reproductive and urinary physiology, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, biology, Arabidopsis Proteins, digestive, oral, and skin physiology, fungi, Calcium-Binding Proteins, Fatty acid, food and beverages, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Articles, Ethylenes, biology.organism_classification, Plant Dormancy, Gibberellins, Oxidative Stress, Biochemistry, chemistry, Fatty Acids, Unsaturated, Gibberellin, Reactive Oxygen Species, 010606 plant biology & botany, Abscisic Acid
Abstract

Contrasting with the wealth of information available on the multiple roles of jasmonates in plant development and defense, knowledge about the functions and the biosynthesis of hydroxylated oxylipins remains scarce. By expressing the caleosin RESPONSIVE TO DESSICATION20 (RD20) in Saccharomyces cerevisiae, we show that the recombinant protein possesses an unusual peroxygenase activity with restricted specificity toward hydroperoxides of unsaturated fatty acid. Accordingly, Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid, a linolenate-derived hydroxide. These plants exhibit elevated levels of reactive oxygen species (ROS) associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity at seed germination. These phenotypes are dependent on the presence of active RD20, since they are abolished in the rd20 null mutant and in lines overexpressing RD20, in which peroxygenase was inactivated by a point mutation of a catalytic histidine residue. RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid. Under oxidative stress, 13-hydroxy-9,11,15-octadecatrienoic acid still accumulates in RD20-overexpressing lines, but this lipid oxidation is associated with reduced ROS levels, minor cell death, and delayed floral transition. A model is discussed where the interplay between fatty acid hydroxides generated by RD20 and ROS is counteracted by ethylene during development in unstressed environments.

Published
2014

36. Quels bioindicateurs et biomarqueurs utiliser pour la surveillance de la qualité des sols et l’évaluation des risques environnementaux?

Author

Guenola Peres, Benjamin Pauget, Thierry Beguiristain, Jean-Jacques Bessoule, Josselin Bodilis, Rémi Chaussod, Daniel Cluzeau, Nathalie Cheviron, Michael Coeurdassier, Jérôme Cortet, Steven Criquet, Samuel Dequiedt, Annette De Vaufleury, Faure, A., Christophe Gangneux, Isabelle Gattin, Mickael Hedde, Adnane Hitmi, Marc Legras, Marina Leguedard, Patrice Lepelletier, Christian Mougin, Sylvie Nelieu, Stéphane Ponton, Lionel Ranjard, Laurence Rougé, Salima Taibi, Franck Vandenbulcke, Cecile Villenave, Laurence Galsomies, Cécile Grand, Antonio Bispo, Sol Agro et hydrosystème Spatialisation (SAS), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), LEB Aquitaine Transfert-ADERA, Morphodynamique Continentale et Côtière (M2C), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), UR 0251 Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés, Institut National de la Recherche Agronomique (INRA)-Santé des plantes et environnement (S.P.E.)-Environnement et Agronomie (E.A.)-Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés (PESSAC), Laboratoire Sols et Environnement (LSE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), ENS des Mines de St-Etienne, Université Jean Monnet [Saint-Étienne] (UJM), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), Laboratoire de PBV, Institut Universitaire de Technologie de Clermont­ Ferrand, Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), Ingénierie Durable, Equipe Ecologie Numérique et Ecotoxicologie, Université de Lille, Sciences et Technologies, Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Elisol Environnement, Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Association Française pour l'Etude du Sol (AFES). FRA., Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Laboratoire Génie Civil et géoEnvironnement LGCgE-Lille 1, Ecologie Numérique et Ecotoxicologie, Université Lille Nord de France (COMUE), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université de Rennes (UR), Laboratoire Chrono-environnement (UMR 6249) (LCE), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Institut National de la Recherche Agronomique (INRA), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure des Mines de St Etienne (ENSM ST-ETIENNE), Sol Agro et hydrosystème Spatialisation ( SAS ), Institut National de la Recherche Agronomique ( INRA ) -AGROCAMPUS OUEST, Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), LEB Aquitaine Transfert – ADERA, Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ) -Santé des plantes et environnement ( S.P.E. ) -Environnement et Agronomie ( E.A. ) -Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés ( PESSAC ), Laboratoire Sols et Environnement ( LSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Centre d’Ecologie Fonctionnelle et Evolutive ( CEFE ), Université Paul-Valéry - Montpellier 3 ( UM3 ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -École pratique des hautes études ( EPHE ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Aix Marseille Université ( AMU ) -Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique ( CNRS ), Université Jean Monnet [Saint-Étienne] ( UJM ), ESITPA, Ecole Nationale d'Ingénieurs des Techniques Agricoles ( ESITPA ), Ecole Superieure des Ingenieurs et Techniciens pour l'Agriculture ( ESITPA ), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] ( EEF ), Institut de Recherche pour le Développement ( IRD ), Université Lille Nord de France, Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes ( Eco&Sols ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Institut de Recherche pour le Développement ( IRD ) -Institut National de la Recherche Agronomique ( INRA ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Agence de l'Environnement et de la Maîtrise de l'Energie ( ADEME ), and Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3)

Subjects
[ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

37. Taxonomy of gymnospermae: multivariate analyses of leaf fatty acid composition

Author

Sébastien Mongrand, Brigitte Patouille, Chantal Lacomblez, Claude Cassagne, Marie Chavent, Jean-Jacques Bessoule, and Alain Badoc

Subjects
Chloroplasts, Larix, Plant Science, Horticulture, Biochemistry, Cedrus, Stangeriaceae, Terminology as Topic, Botany, Molecular Biology, Phylogeny, biology, Galactolipids, Fatty Acids, Discriminant Analysis, Zamiaceae, General Medicine, Pinaceae, biology.organism_classification, Lipids, Ginkgoaceae, Plant Leaves, Cycadopsida, Cephalotaxaceae, Multivariate Analysis, Glycolipids, Taxaceae, Podocarpaceae, Abies
Abstract

The fatty acid composition of photosynthetic tissues from 137 species of gymnosperms belonging to 14 families was determined by gas chromatography. Statistical analysis clearly discriminated four groups. Ginkgoaceae, Cycadaceae, Stangeriaceae, Zamiaceae, Sciadopityaceae, Podocarpaceae, Cephalotaxaceae, Taxaceae, Ephedraceae and Welwitschiaceae are in the first group, while Cupressaceae and Araucariaceae are mainly in the second one. The third and the fourth groups composed of Pinaceae species are characterized by the genera Larix, and Abies and Cedrus, respectively. Principal component and discriminant analyses and divisive hierarchical clustering analysis of the 43 Pinaceae species were also performed. A clear-cut separation of the genera Abies, Larix, and Cedrus from the other Pinaceae was evidenced. In addition, a mass analysis of the two main chloroplastic lipids from 14 gymnosperms was performed. The results point to a great originality in gymnosperms since in several species and contrary to the angiosperms, the amount of digalactosyldiacylglycerol exceeds that of monogalactosyldiacylglycerol.

Published
2001

38. Mutagenesis of a plastidial lysophosphatidic acid acyltransferase

Author

Thomas Roscoe, Jean-Jacques Bessoule, Michel Delseny, René Lessire, and Sylvie Maisonneuve

Subjects
Alanine, Binding Sites, Mutagenesis, Mutant, Brassica, Templates, Genetic, Biology, Biochemistry, Recombinant Proteins, Isoenzymes, Complementation, Microsomes, Gene expression, Escherichia coli, Mutagenesis, Site-Directed, Moiety, Amino Acid Sequence, Plastids, Acyltransferases, Histidine, Function (biology), Sequence Deletion
Abstract

A combination of site-directed and random mutagenesis generated sequence variants of a plastidial lysophosphatidic acid acyltransferase. Alanine substitutions of residues present within two conserved motifs including the putative catalytic histidine resulted in a loss of acyltransferase activity assessed as complementation competance. Substitutions at five sites within the central core resulted in reduced or loss of activity. Truncation mutants reveal that sequences in the C-terminal moiety are essential for function.

Published
2000

39. Import of Lyso-Phosphatidylcholine into Chloroplasts Likely at the Origin of Eukaryotic Plastidial Lipids

Author

Claude Cassagne, Sébastien Mongrand, and Jean-Jacques Bessoule

Subjects
Chloroplasts, Physiology, Biological Transport, Active, Plant Science, Biology, Allium, chemistry.chemical_compound, Biosynthesis, Phosphatidylcholine, Genetics, Glycerol, Plastids, Plastid, chemistry.chemical_classification, Galactolipids, Fatty Acids, Lysophosphatidylcholines, Fatty acid, Lipid metabolism, Lipid Metabolism, Chloroplast, Kinetics, Eukaryotic Cells, Biochemistry, chemistry, Glycolipids, Research Article
Abstract

Plastids rely on the import of extraplastidial precursor for the synthesis of their own lipids. This key phenomenon in the formation of plastidial phosphatidylcholine (PC) and of the most abundant lipids on earth, namely galactolipids, is poorly understood. Various suggestions have been made on the nature of the precursor molecule(s) transferred to plastids, but despite general agreement that PC or a close metabolite plays a central role, there is no clear-cut answer to this question because of a lack of conclusive experimental data. We therefore designed experiments to discriminate between a transfer of PC, 1-acylglycero phosphorylcholine (lyso-PC), or glycerophosphorylcholine. After pulse-chase experiments with glycerol and acetate, plastids of leek (Allium porrum L.) seedlings were purified. The labels of the glycerol moiety and the sn-1- andsn-2-bound fatty acids of plastidial lipids were determined and compared with those associated with the extraplastidial PC. After import, plastid lipids contained the glycerol moiety and the fatty acids esterified to the sn-1 position originating from the extraplastidial PC; no import of sn-2-bound fatty acid was detected. These results rule out a transfer of PC or glycerophosphorylcholine, and are totally explained by an import of lyso-PC molecules used subsequently as precursor for the synthesis of eukaryotic plastid lipids.

Published
2000

40. Involvement of Lipoxygenase-dependent Production of Fatty Acid Hydroperoxides in the Development of the Hypersensitive Cell Death induced by Cryptogein on Tobacco Leaves

Author

Philippe Etienne, Anja Knoll, Jean-Pierre Blein, Jean-Jacques Bessoule, Lydie Suty, Christian Triantaphylidès, Jean-Pierre Agnel, Jean-Luc Montillet, Christine Rusterucci, Béatrice Alonso, Christine Battesti, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux Ségalen [Bordeaux 2], Phytopharmacie et Biochimie des Iteractions Cellulaires (PBIC), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), and Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)

Subjects
feuille, 0106 biological sciences, Lipid Peroxides, Programmed cell death, Necrosis, hypersensibilité, réaction hs, Lipoxygenase, nicotiana tabacum, Biology, 01 natural sciences, Biochemistry, Fungal Proteins, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, cryptogénie, Tobacco, medicine, peroxyde, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Lipase, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Cell Death, acide gras, Algal Proteins, food and beverages, Cell Biology, Respiratory burst, tabac, Chloroplast, Plants, Toxic, chemistry, biology.protein, lipids (amino acids, peptides, and proteins), peroxydation, medicine.symptom, Signal Transduction, 010606 plant biology & botany, Polyunsaturated fatty acid
Abstract

Lipid peroxidation was investigated in relation with the hypersensitive reaction in cryptogein-elicited tobacco leaves. A massive production of free polyunsaturated fatty acid (PUFA) hydroperoxides dependent on a 9-lipoxygenase (LOX) activity was characterized during the development of leaf necrosis. The process occurred after a lag phase of 12 h, was accompanied by the concomitant increase of 9-LOX activity, and preceded by a transient accumulation of LOX transcripts. Free radical-mediated lipid peroxidation represented 10% of the process. Inhibition and activation of the LOX pathway was shown to inhibit or to activate cell death, and evidence was provided that fatty acid hydroperoxides are able to mimic leaf necrotic symptoms. Within 24 h, about 50% of leaf PUFAs were consumed, chloroplast lipids being the major source of PUFAs. The results minimize the direct participation of active oxygen species from the oxidative burst in membrane lipid peroxidation. They suggest, furthermore, the involvement of lipase activity to provide the free PUFA substrates for LOX. The LOX-dependent peroxidative pathway, responsible for tissue necrosis, appears as being one of the features of hypersensitive programmed cell death.

Published
1999

41. Transfer and subsequent metabolism of lysolipids studied by immobilizing subcellular compartments in alginate beads

Author

Claude Cassagne, Eric Testet, Nicole Verdoni, and Jean-Jacques Bessoule

Subjects
Alginates, Hexuronic Acids, Substrate (chemistry), Cell Biology, Compartment (chemistry), Metabolism, Biology, Lipid Metabolism, Phosphatidylcholine Biosynthesis, chemistry.chemical_compound, Acyl-CoA, Membrane, Lysophosphatidylcholine, Glucuronic Acid, Biochemistry, chemistry, Phosphatidylcholine, Electrophoresis, Polyacrylamide Gel, Molecular Biology, Subcellular Fractions
Abstract

The transfer and subsequent metabolism of lysophosphatidylcholine between subcellular compartments were studied in vitro by embedding membranes in alginate beads. After several experiments to validate the process, it was demonstrated that lysophosphatidylcholine was transferred from microsomes embedded in alginate beads to immobilized chloroplasts, that this transfer involved the partition of this molecule, and that the imported lysophosphatidylcholine was further used as substrate for phosphatidylcholine biosynthesis. More generally, the technique used makes it possible to avoid any cross-contamination between compartments, to evidence a transfer of molecules, and to study the metabolism of the imported molecules in the acceptor compartment.

Published
1999

42. Dehydration of 3-hydroxyacyl-CoA in brain very-long-chain fatty acid synthesis

Author

Jean-Jacques Bessoule, Anja Knoll, Françoise Sargueil, and Claude Cassagne

Subjects
Fatty Acid Desaturases, Very long chain fatty acid, Reductase, Medicinal chemistry, Gas Chromatography-Mass Spectrometry, Reversible reaction, Substrate Specificity, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Hydrolysis, Acyl-CoA Dehydrogenases, Microsomes, Spectroscopy, Fourier Transform Infrared, medicine, Animals, Organic chemistry, Coenzyme A, Dehydration, Rats, Wistar, Hydro-Lyases, chemistry.chemical_classification, Chemistry, Fatty Acids, Brain, Water, Substrate (chemistry), Esters, Cell Biology, medicine.disease, Rats, Kinetics, Enzyme, Microsome, lipids (amino acids, peptides, and proteins), NADP
Abstract

Rat brain microsomes actively dehydrate 3-hydroxyacyl-CoAs. Using chemically synthesized [1- 14 C] (R,S) 3-hydroxyeicosanoyl-CoA, we investigated the biochemical characteristics of the dehydration and reduction steps of stearoyl-CoA elongation. The reaction products, separated and identified as trans2,3-enoyl-CoAs and, in the presence of NADPH, as saturated acyl-CoAs, were released from the enzyme as thioesters which were partly hydrolysed. A kinetic analysis of the two coupled reactions showed that the 3-hydroxyacyl-CoA dehydrase catalysed a reversible reaction with kinetic constants of about 0.045 min −1 for forward reaction (dehydration) and 0.025 min −1 for reverse reaction (hydration); V max of the dehydration reached 20 nmoles/min/mg and the apparent Km was 44 μM. In the presence of NADPH, the kinetic constants for the dehydrase were unchanged and that for the trans2,3-enoyl-CoA reductase was 0.025 min −1 . The relative proportion of trans2,3-enoyl-CoA and saturated acyl-CoA depended on the protein amount. An inhibition of the reduction step was observed for substrate concentrations above 15 μM. The 3-hydroxyacyl-CoA dehydrase used (R) rather than (S) 3-hydroxyacyl-CoA. Furthermore, the elongation of (R) 3-hydroxyeicosanoyl-CoA yielded saturated very-long-chain acyl-CoA. These results demonstrated that 3-hydroxyacyl-CoAs entered the elongating complex exclusively at the level of the dehydrase and not of the condensing enzyme.

Published
1999

43. Lipid trafficking in plant cells

Author

Patrick Vincent, Eric Testet, Sébastien Mongrand, Claude Cassagne, Patrick Moreau, and Jean-Jacques Bessoule

Subjects
Organelles, chemistry.chemical_classification, biology, Chemistry, Cell Membrane, Fatty acid, Biological Transport, Lipid metabolism, Cell Biology, Plants, Lipid Metabolism, Models, Biological, Biochemistry, Fatty acid synthase, Lipid droplet, Free fatty acid receptor, biology.protein, Beta-ketoacyl-ACP synthase, adipocyte protein 2, Polyunsaturated fatty acid
Published
1998

44. Le métabolisme fongique dans l’interaction hôte– Candida : exemple de la β-oxydation des acides gras chez Candida lusitaniae

Author

Thierry Noël, Jean-Jacques Bessoule, Isabelle Accoceberry, Karine Dementhon, Marine Lucas-Guerin, Sabine Charron, Frédéric Gabriel, Sandrine Albac, Stéphen Manon, and Bénédicte Salin

Subjects
Infectious Diseases
Abstract

Introduction Les cellules phagocytaires des lignees monocytaire et neutrophile sont essentielles a la defense de l’organisme au cours d’une infection a Candida spp. Cependant in vitro, C. albicans , C. glabrata et C. lusitaniae sont capables de resister a la phagocytose. Notre hypothese est que les lipides, et en particulier les acides gras, pourraient constituer une ressource energetique privilegiee pour la levure afin de resister a la phagocytose. Chez les levures Ascomycetes, le catabolisme des acides gras est presume etre exclusivement localise dans les peroxysomes et repose principalement sur la β-oxydation. Methodes Nous avons construit par recombinaison homologue chez C. lusitaniae 11 mutants nuls (simples, doubles ou triples) afin de bloquer la β-oxydation peroxysomale (genes FOX1 , FOX2 , FOX3 ), mais aussi en amont l’import peroxysomal des acides gras a longue chaine carbonee (gene PXA1 ) et en aval le cycle du glyoxylate (gene ICL1 ). La caracterisation du phenotype des mutants obtenus a compris l’etude : – de l’assimilation des sources de carbone ; – de la consommation d’un substrat radioactif de la β-oxydation par des extraits bruts et fractions cellulaires ; – de l’immunolocalisation de la proteine Fox2 (western blot sur fractions cellulaires, microscopie electronique) ; – de la resistance in vitro a la phagocytose macrophagique (modele developpe au laboratoire) ; – de leur virulence dans un modele murin de candidemie. Resultats Contrairement a ce qui est rapporte pour C. albicans , l’inactivation du gene FOX2 (ou des genes FOX1 ou FOX3 ) ne se traduit pas par un blocage du catabolisme des acides gras chez C. lusitaniae . Nos travaux ont permis d’expliquer ce phenotype original par l’existence de 3 voies pour le catabolisme des acides gras chez C. lusitaniae et de demontrer la colocalisation inedite chez les Eucaryotes de la proteine Fox2 au peroxysome et a la mitochondrie. A 6 h d’infection, la survie des macrophages infectes par le mutant fox2Δ etait comprise entre celle des macrophages infectes par la souche sauvage (la plus faible) et celle des macrophages infectes par le mutant icl1Δ (la plus forte). Les mutants icl1Δ et fox2Δ se multipliaient moins que la souche sauvage. La virulence in vivo du mutant icl1Δ etait attenuee, alors que celle du mutant fox2Δ n’etait pas differente de la souche sauvage. Conclusion Le cycle peroxysomal de l’acide glyoxylique est indispensable pour la virulence de C. lusitaniae , et l’acetyl-CoA qui l’alimente ne provient pas seulement de la s-oxydation des acides gras.

Published
2015

45. The C16:3\C18:3 fatty acid balance in photosynthetic tissues from 468 plant species

Author

Claude Cassagne, Francis Cabantous, Jean-Jacques Bessoule, and Sébastien Mongrand

Subjects
chemistry.chemical_classification, Plant evolution, Plant physiology, Fatty acid, Plant Science, General Medicine, Horticulture, Biology, Photosynthesis, Biochemistry, Chloroplast, Metabolic pathway, chemistry, Chemotaxonomy, Botany, Molecular Biology, Unsaturated fatty acid
Abstract

Two kinds of plants may be distinguished according to their (n–3) trienoic fatty acid composition in photosynthetic tissues. The cis -7,10,13-hexadecatrienoic acid\ cis -9,12,15-octadecatrienoic acid balance directly reflects the biosynthesis pathways (a plastidial one and an extra-plastidial one) of chloroplastic lipids. We analysed the correlation between the existence of these pathways and the evolutionary classification of Cormophytes (particularly Angiosperms). By using cis -7,10,13-hexadecatrienoic acid as a marker for the existence of the plastidial pathway, we studied the overall fatty acid composition of 468 plant species (280 already described in the literature and 188 new ones) distributed among 141 botanical families. The data strongly suggest that the plastidial pathway was lost during evolution and that, in the case of dicotyledonous plants, this loss probably occurred independently and at different rates. The data are also discussed from an environmental and chemotaxonomic point of view.

Published
1998

46. Host-pathogen interaction and signaling molecule secretion are modified in the dpp3 knockout mutant of Candida lusitaniae

Author

Thierry Noël, Ayman Sabra, Karine Dementhon, Jean-Jacques Bessoule, Vessela Atanasova-Penichon, Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biogenèse membranaire (LBM), Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), and French Ministere de l'Enseignement Superieur et de la Recherche

Subjects
Cell signaling, [SDV]Life Sciences [q-bio], Immunology, Mutant, Virulence, Nitric Oxide, Microbiology, Gene Knockout Techniques, Mice, Animals, Secretion, Gene Silencing, Dipeptidyl-Peptidases and Tripeptidyl-Peptidases, Candida, Cellular Microbiology: Pathogen-Host Cell Molecular Interactions, biology, Tumor Necrosis Factor-alpha, Candida lusitaniae, Macrophages, Phenylethyl Alcohol, biology.organism_classification, Farnesol, Reverse genetics, Cell biology, Interleukin-10, Interleukin 10, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, Biochemistry, Host-Pathogen Interactions, Parasitology, Reactive Oxygen Species
Abstract

Candida lusitaniae is an emerging opportunistic yeast and an attractive model to discover new virulence factors in Candida species by reverse genetics. Our goal was to create a dpp3 Δ knockout mutant and to characterize the effects of this gene inactivation on yeast in vitro and in vivo interaction with the host. The secretion of two signaling molecules in Candida species, phenethyl alcohol (PEA) and tyrosol, but not of farnesol was surprisingly altered in the dpp3 Δ knockout mutant. NO and reactive oxygen species (ROS) production as well as tumor necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) secretion were also modified in macrophages infected with this mutant. Interestingly, we found that the wild-type (WT) strain induced an increase in IL-10 secretion by zymosan-activated macrophages without the need for physical contact, whereas the dpp3 Δ knockout mutant lost this ability. We further showed a striking role of PEA and tyrosol in this modulation. Last, the DPP3 gene was found to be an essential contributor to virulence in mice models, leading to an increase in TNF-α secretion and brain colonization. Although reinsertion of a WT DPP3 copy in the dpp3 Δ knockout mutant was not sufficient to restore the WT phenotypes in vitro , it allowed a restoration of those observed in vivo . These data support the hypothesis that some of the phenotypes observed following DPP3 gene inactivation may be directly dependent on DPP3 , while others may be the indirect consequence of another genetic modification that systematically arises when the DPP3 gene is inactivated.

Published
2013

47. Influence of fine process particles enriched with metals and metalloids on Lactuca sativa L. leaf fatty acid composition following air and/or soil-plant field exposure

Author

Marina Le Guédard, Eva Schreck, Christophe Laplanche, Camille Dumat, Annabelle Austruy, Tiantian Xiong, Jean-Jacques Bessoule, Yann Foucault, Centre National d'Études Spatiales - CNES (FRANCE), Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut de Recherche pour le Développement - IRD (FRANCE), Société de Traitement Chimique des Métaux - STCM (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Université de Bordeaux 2 - Victor Segalen (FRANCE), Laboratoire Ecologie Fonctionnelle et Environnement (ECOLAB), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS), Géosciences Environnement Toulouse (GET), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ecologie Fonctionnelle et Environnement (LEFE), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), and Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)

Subjects
Health, Toxicology and Mutagenesis, 0211 other engineering and technologies, Lactuca, 02 engineering and technology, 010501 environmental sciences, Toxicology, 01 natural sciences, Soil, Magnoliopsida, Mixture of metal(loid)s, Soil Pollutants, Metalloids, 0105 earth and related environmental sciences, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, chemistry.chemical_classification, Ecologie, Environnement, Air Pollutants, 021110 strategic, defence & security studies, biology, Fatty Acids, Fatty acid, Soil chemistry, food and beverages, General Medicine, Lettuce, Contamination, biology.organism_classification, Statistical index, Pollution, Plant Leaves, chemistry, Metals, 13. Climate action, Environmental chemistry, Soil water, Phytotoxicity, Metalloid, Uptake pathways, Leaf fatty acid composition, Environmental Monitoring
Abstract

International audience; We investigate the effect of both foliar and root uptake of a mixture of metal(loid)s on the fatty acid composition of plant leaves. Our objectives are to determine whether both contamination pathways have a similar effect and whether they interact. Lactuca sativa L. were exposed to fine process particles enriched with metal(loid)s in an industrial area. Data from a first experiment were used to conduct an exploratory statistical analysis which findings were successfully cross-validated by using the data from a second one. Both foliar and root pathways impact plant leaf fatty acid composition and do not interact. Z index (dimensionless quantity), weighted product of fatty acid concentration ratios was built up from the statistical analyses. It provides new insights on the mechanisms involved in metal uptake and phytotoxicity. Plant leaf fatty acid composition is a robust and fruitful approach to detect and understand the effects of metal(loid) contamination on plants.

Published
2013

48. Are plants useful as accumulation indicators of metal bioavailability?

Author

E. Remon, Olivier Faure, Jean-Jacques Bessoule, J.-L. Bouchardon, Cyrille Conord, M. Le Guedard, Centre Sciences des Processus Industriels et Naturels (SPIN-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Maison de l'eau et de la pêche de la Corrèze (MEP 19), Maison de l'eau et de la pêche de la Corrèze, Département Géochimie, environnement, écoulement, réacteurs industriels et cristallisation (GENERIC-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-SPIN, Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Département GéoSciences et Environnement (GSE-ENSMSE), Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR-5200, and LEB Aquitaine Transfert-ADERA

Subjects
Health, Toxicology and Mutagenesis, 010501 environmental sciences, Toxicology, 01 natural sciences, Metallurgical landfill, Metal, chemistry.chemical_compound, Soil, Soil contamination, Phytoavailability, Soil Pollutants, Ammonium, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, 0105 earth and related environmental sciences, Chemistry, food and beverages, Plant community, 04 agricultural and veterinary sciences, General Medicine, Vegetation, 15. Life on land, Contamination, Plants, Pollution, Metal bioavailability, Bioindication, Metals, visual_art, Environmental chemistry, 040103 agronomy & agriculture, visual_art.visual_art_medium, 0401 agriculture, forestry, and fisheries, Bioindicator, Environmental Monitoring
Abstract

International audience; The use of accumulation bioindicator to assess metal bioavailability has mainly concerned individual species. This work addresses this issue at the plant community level. Metal content within different species from plant communities found at three contaminated and one uncontaminated site was compared. Results showed that for two contaminated sites, leaf metals concentrations were comparable to those in plants from control site, i.e. approx (mg/kg) 0.1 Cd, 0.2 Cr, 9.2 Cu, 1.8 Ni, 0.5 Pb and 42 Zn. Only plants from the third site showed higher metal contents, ranging from 1.5- to 8-fold those of the control community. This contrasted with ammonium acetate-EDTA extractions, which indicated a very high "availability" of metals at the three sites, as compared to the control site. Thus, metal content in plant communities provided accurate information on actual transfer toward the ensemble of vegetation, which could be used to establish site-specific "fingerprints" of metal bioavailability.

Published
2013

49. Les enseignements du programme ADEME « Bioindicateurs de l’état biologique des sols »

Author

Antionio Bispo, Guenola Peres, Mickael Hedde, Isabelle Gattin, Daniel Cluzeau, Nathalie Cheviron, Jennifer Harris-Hellal, Marina Le Guédard, Jean-Jacques Bessoule, Nuria Ruiz, Benjamin Pauget, Annette de Vaufleury, Christian Mougin, Thierry Beguiristain, Lionel Ranjard, Samuel Dequiedt, Rémi Chaussod, Olivier Faure, Adnane Hitmi, Steven Criquet, Marc Legras, Nicolas Laurent, Franck Vandenbulcke, Karine Laval, Christophe Gangneux, Nicolas, E., Michael Coeurdassier, Stéphane Ponton, Jérôme Cortet, Cécile Villenave, Josselin Bodilis, Laurence Rougé, Salima Taïbi, C Thoisy-Dur, J., Jeanne Bodin, Aude Alaphilippe, Francis Douay, Sabine Houot, Muriel Guernion, Patrice Lepelletier, Cedric Repinçay, Jean-François Vian, Sébastien Conil, Noële Raoult, Pierre Plassart, Vincent Mercier, Hotte, H., Laurence Galsomiès, Cécile Grand, Agence de l'Environnement et de la Maîtrise de l'Energie ( ADEME ), Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), Physico-chimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés ( PESSAC ), Institut National de la Recherche Agronomique ( INRA ), Ecole d'Ingénieurs en Agriculture, ESITPA, Université de Rennes ( UNIV-RENNES ), Equipe de Biogéochimie Environnementale, Bureau de Recherches Géologiques et Minières ( BRGM ), UMR 5200, INRA Bordeaux Aquitaine, LEB Aquitaine Transfert – ADERA, Université Bordeaux Segalen - Bordeaux 2, Centre France-Nord, UMR 211 BIOEMCO, Institut de Recherche pour le Développement ( IRD [France-Ouest] ), Department of Chrono-Environment, UMR UFC/CNRS 6249 USC INRA, Université de Franche-Comté ( UFC ), Department of Chrono-Environment, University of Franche-Comté, UMR UFC/CNRS 6249 USC INRA, Laboratoire des Interactions Microorganismes-Minéraux-Matière Organique dans les sols ( LIMOS ), Université Henri Poincaré - Nancy 1 ( UHP ) -Centre National de la Recherche Scientifique ( CNRS ), Agroécologie [Dijon], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), UMR CNRS 5600 EVS-EMSE-Géosciences et Environnement F 42, Ecole Nationale Supérieure des Mines de Saint-Etienne, Université de Lyon (COMUE), Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant - Clermont Auvergne ( PIAF ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Centre National de la Recherche Scientifique ( CNRS ) -Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université ( AMU ) -Université d'Avignon et des Pays de Vaucluse ( UAPV ), Systèmes d'élevage méditerranéens et tropicaux ( UMR SELMET ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Institut National de la Recherche Agronomique ( INRA ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] ( EEF ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Laboratoire de Microbiologie Signaux et Microenvironnement ( LMSM ), Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Unité de Recherches Intégrées - Gotheron ( GOTH RI UERI ), Equipe Sols et Environnement, Laboratoire Génie Civil et géoEnvironnement Lille Nord, Institut Supérieur d'Agriculture de Lille (Groupe ISA), Environnement et Grandes Cultures ( EGC ), AgroParisTech-Institut National de la Recherche Agronomique ( INRA ), Centre National de la Recherche Scientifique ( CNRS ), UR 0251 PESSAC Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés, Département AGEP (AGroécosystèmes-Environnement-Productions), isaralyon, AGRAPOLE, AGEP, Observ Perenne Environm, DRD Observat Surveillance, Agence Nationale pour la Gestion des Déchets Radioactifs ( ANDRA ), Laboratoire Sols et Environnement ( LSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Institut National de la Recherche Agronomique (INRA), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), Université de Rennes (UR), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Laboratoire de biogenèse membranaire (LBM), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Biogéochimie et écologie des milieux continentaux (Bioemco), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire des Interactions Microorganismes-Minéraux-Matière Organique dans les sols (LIMOS), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Environnement, Ville, Société (EVS), École normale supérieure de Lyon (ENS de Lyon)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-École Nationale des Travaux Publics de l'État (ENTPE)-École nationale supérieure d'architecture de Lyon (ENSAL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Systèmes d'élevage méditerranéens et tropicaux (UMR SELMET), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Unité Expérimentale Recherches Intégrées - Gotheron (UERI), Institut Supérieur d'Agriculture de Lille (ISA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Environnement et Grandes Cultures (EGC), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre National de la Recherche Scientifique (CNRS), AGroécosystèmes Environnement Productions (AGEP), ISARA-Lyon, Agence Nationale pour la Gestion des Déchets Radioactifs (ANDRA), Laboratoire Sols et Environnement (LSE), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Physico-chimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Lyon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant - Clermont Auvergne (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Unité de Recherches Intégrées - Gotheron (GOTH RI UERI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
[ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

Les enseignements du programme ADEME « Bioindicateurs de l’état biologique des sols ». Séminaire national du « Programme Inventaire, Gestion et Conservation des Sols (IGCS) »

Published
2013

50. Occurrence of an acyl-CoA:1-acylglycerophosphorylcholine acyltransferase in plant mitochondria

Author

Eric Testet, Thérèse Guillot-Salomon, Sébastien Mongrand, Claude Cassagne, Catherine Cantrel, and Jean-Jacques Bessoule

Subjects
Acyltransferase, Biophysics, Lysophospholipids, Mitochondrion, Biochemistry, chemistry.chemical_compound, Acyl-CoA, Structural Biology, Phosphatidylcholine, Genetics, Molecular Biology, Lysophosphatidylcholine, Endoplasmic reticulum, 1-Acylglycerophosphocholine O-Acyltransferase, Lysophosphatidylcholines, Plant, Cell Biology, Plants, Lipid, Mitochondria, chemistry, Phosphatidylcholines, lipids (amino acids, peptides, and proteins), Bacterial outer membrane, 1-Acylglycerophosphocholine 0-acyl-transferase (EC 2.3-1.23)
Abstract

In the presence of oleoyl-CoA, purified and intact mitochondria from potato tuber formed phosphatidylcholine from labeled lysophosphatidylcholine. The labeled oleoyl moiety of the acyl-CoA was also incorporated in the absence of exogenous lysolipids, such incorporation being largely increased by the addition of exogenous lysophosphatidylcholine. In the presence of various other lysophospholipids, no synthesis of the corresponding phospholipids was observed, suggesting a high specificity of the acyltransferase towards the acyl acceptor. This enzyme was chiefly located in the outer membrane of mitochondria. These results indicate that any acylglycerophosphorylcholine transferred from the endoplasmic reticulum to mitochondria may be acylated to phosphatidylcholine.

Published
1996

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