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In day-to-day practice pediatric orthopedic surgeons often come up against the question of sport. The aim of the present study was to analyze the relationship between sport and childhood, with 3 questions: (1) What are the benefits of sport for children? (2) How to manage high-level child athletes? And (3) What sports are possible after major orthopedic surgery? Sports provide many benefits for children, and are to be encouraged. Sixty minutes' moderate to intense physical activity per day benefits motor development and bone mineralization and reduces the risk of obesity. On the other hand, excessive sports activity, as encountered in high-level sport, can be harmful for the child's development. The amount of training should not exceed a certain threshold in terms of hours per week according to age. Surgical treatment of sport-related traumatic lesions does not necessarily accelerate return to sport: indications need to be reasonable, despite pressure from the patient's circle. Sports are possible after major orthopedic surgery, although return to sport tends to be delayed and the level is lower than preoperatively.

Les calcifications discales sont le plus souvent révélées chez l'enfant, par des crises douloureuses rachidiennes. Leur localisation cervicale, semble être la plus fréquente. Cependant, les autres localisations sont moins symptomatiques et pourraient ainsi passer inaperçues. Nous rapportons deux observations d'enfants hospitalisés pour des calcifications discales cervicales symptomatiques. Il s'agit : du cas d'une fillette âgée de quatre ans et demi ayant présenté un torticolis, révélateur de la migration d'une calcification discale jusque-là asymptomatique ; et de celui du garçon âgé de 05 ans venu pour cervicalgie aigue et chez qui une calcification discale a été retrouvée. Leur traitement était essentiellement conservateur. Les calcifications discales intervertébrales de l'enfant sont une pathologie rare dont l'étiopathogénie reste inconnue. Leur diagnostic ne doit pas se faire sans un examen neurologique attentif et une imagerie devant des douleurs rachidiennes inexpliquées chez l'enfant.The Pan African Medical Journal 2016;25

International audience; The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at the cell surface through a short-lived process. For transmission electron microscopy (TEM) studies of the intracellular ultrastructure of the small number of cells releasing viral particles at a given time, we developed a new correlative microscopy method: CSEMTEM (for correlative scanning electron microscopy - transmission electron microscopy). CSEMTEM analysis suggested that chimeric flavivirus particles were released as individual particles, in small exocytosis vesicles, via a regulated secretory pathway. Our morphological findings provide new insight into interactions between flaviviruses and cells and demonstrate that CSEMTEM is a useful new method, complementary to SEM observations of biological events by intracellular TEM investigations.

A full-term baby boy required moderate resuscitation at birth. Clinical examination revealed a painless lump of the right clavicle. Radiographically, the borders of the two fragments were sclerotic (Fig. 1) and follow-up US showed no callus formation. These findings confirmed the diagnosis of congenital pseudarthrosis of the clavicle (CPC). CPC results from failed coalescence of the two primary ossification centres of the clavicle; the true mechanism of this condition remains unknown [1, 2]. It occurs almost always on the right side and very rarely bilaterally. The larger medial segment always lies above the smaller lateral segment. On both sides of the pseudarthrosis, features of enchondral ossification and fibrocartilage are found [1]. Diagnosis in the neonatal period has been rarely reported, with the lesion usually found during the first 2 years of life [2]. The function of the shoulder is not impaired, although an non-aesthetic painful deformity or thoracic outlet syndrome may occur during and/or after the growth period. Surgery, consisting of resection, bone grafting and osteosynthesis is usually performed at a mean of 6 years of age [1]. There are two differential diagnoses. Cleidocranial dysostosis is easily excluded by the absence of hypoplastic clavicles and a wide pubic symphysis. It is important to differentiate CPC from birth trauma; in the latter, callus formation occurs very quickly.

The diagnosis of scoliosis is based on the association of kyphosis, vertebral rotation, and lateral deviation of the spine. The clinical examination has to be rigorous in order to rule out a potential etiology for this tridimensional deformity. The assessment of the growth, and of the progressive nature of the scoliosis, is essential before any treatment. For minor deformities, a simple clinical monitoring will be necessary. On the other hand, for moderate deformities, a non-surgical treatment with brace will be recommended. Finally, for severe deformities, the treatment will be a surgical treatment. Surgery will consist of a spinal arthrodesis.

International audience; The necessity to perform serum-free cultures to produce recombinant glycoproteins generally requires an adaptation procedure of the cell line to new environmental conditions, which may therefore induce quantitative and qualitative effects on the product, particularly on its glycosylation. In previous studies, desialylation of EPO produced by CHO cells was shown to be dependent on the presence of serum in the medium. In this paper, to discriminate between the effects of the adaptation procedure to serum-free medium and the effects of the absence of serum on EPO production and glycosylation, adapted and non-adapted CHO cells were grown in serum-free and serum-containing media. The main kinetics of CHO cells were determined over batch processes as well as the glycosylation patterns of produced EPO by HPCE-LIF. A reversible decrease in EPO production was observed when cells were adapted to SFX-CHOTM medium, as the same cells partially recovered their production capacity when cultivated in serum-containing medium or in the enriched SFMTM serum-free medium. More interestingly, EPO desialylation that was not observed in both serum-free media was restored if the serum-independent cells were recultured in presence of serum. In the same way, while the serum-independent cells did not release a sialidase activity in both serum-free media, a significant activity was recovered when serum was added. In fact, the cell adaptation process to serum-free conditions did not specifically affect the sialidase release and the cellular mechanism of protein desialylation, which appeared to be mainly related to the presence of serum for both adapted and non-adapted cells.

Forty-two children with nontuberculous spondylodiscitis treated between 1966 and 1997 were reviewed, and the clinical, paraclinical, and therapeutic results are presented. The study shows the difficulties of diagnosis and understanding the pathophysiology of the disease. Additional information is provided by new imaging techniques, disc aspiration, and biopsy. The mean age at treatment was 4 years 6 months. The initial clinical presentation was often misleading and the diagnosis was often delayed (42 days average). Standard radiographs and technetium bone scans were important for diagnosis and patient follow-up. Magnetic resonance imaging and needle aspiration of the disc gave an additional reliable aid in differential diagnosis and helped to guide treatment. Bacteria were isolated in 22 of the 35 samples taken (55% Staphylococcus aureus, 27% Kingella kingae; Coxiella burnetii in one sample). The functional outcome is good if treatment is properly carried out. Disc fibrosis and occasional vertebral fusion develop inevitably in the long term. According to these results, nontuberculous spondylodiscitis is truly osteomyelitis of the spine.