15 results on '"Jeremias, Wander de Jesus"'
Search Results
2. Immunogenicity and safety of inactivated SARS-CoV-2 vaccine (CoronaVac) using two-dose primary protocol in children and adolescents (Immunita-002, Brazil): A phase IV six-month follow up
- Author
-
Corsini, Camila Amormino, primary, Martins, Priscila Fernanda da Silva, additional, Filgueiras, Priscilla Soares, additional, Lourenço, Adelina Júnia, additional, Lima, Ana Esther de Souza, additional, Gomes, Sarah Vieira Contin, additional, Jeremias, Wander de Jesus, additional, Alves, Pedro Augusto, additional, Fernandes, Gabriel da Rocha, additional, Castro, Luciana Lisboa Mota e, additional, Teixeira-Carvalho, Andrea, additional, Campi-Azevedo, Ana Carolina, additional, Curimbaba, Caroline De Almeida Leitao, additional, Lorencini, Daniela Aparecida, additional, Junior, Eolo Morandi, additional, Silva, Victor Mattos da, additional, Cervi, Maria Célia, additional, Borges, Marcos de Carvalho, additional, Nogueira, Maurício Lacerda, additional, Campos, Guilherme Rodrigues Fernandes, additional, Correa, Paulo Roberto Lopes, additional, Carvalho, Taciana Malheiros Lima, additional, Reis, Jordana Grazziela Alves Coelho dos, additional, Reis, Erik Vinicius de Sousa, additional, Castilho, Leda dos Reis, additional, de Lima, Poliana Remundini, additional, Nascimento, João Paulo Resende do, additional, de Oliveira, Jaquelline Germano, additional, Martins-Filho, Olindo Assis, additional, Grenfell, Rafaella Fortini Queiroz e, additional, and Team, Immunita, additional
- Published
- 2024
- Full Text
- View/download PDF
3. Rapid antigen test as a tool for the identification of SARS-CoV-2 infection and its potential as a self-testing device
- Author
-
Filgueiras, Priscilla Soares, primary, Corsini, Camila Amormino, additional, Almeida, Nathalie Bonatti Franco, additional, Pedrosa, Maria Luysa Camargos, additional, Miranda, Daniel Alvim Pena de, additional, Gomes, Sarah Vieira Contin, additional, Assis, Jéssica Vieira de, additional, Silva, Raphael Antônio, additional, Medeiros, Maria Izabella Vieira de Assis Rocha Carvalho de, additional, Lourenço, Adelina Junia, additional, Bicalho, Cecilia Maria Florencio, additional, Vilela, Raquel Virginia Rocha, additional, Jeremias, Wander de Jesus, additional, Fernandes, Gabriel da Rocha, additional, and Queiroz, Rafaella Fortini Grenfell e, additional
- Published
- 2023
- Full Text
- View/download PDF
4. Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata
- Author
-
Queiroz, Fábio Ribeiro, primary, Portilho, Laysa Gomes, additional, Jeremias, Wander de Jesus, additional, Babá, Élio Hideo, additional, do Amaral, Laurence Rodrigues, additional, Silva, Luciana Maria, additional, Coelho, Paulo Marcos Zech, additional, Caldeira, Roberta Lima, additional, and Gomes, Matheus de Souza, additional
- Published
- 2020
- Full Text
- View/download PDF
5. Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
- Author
-
Silva-Moraes, Vanessa, primary, Shollenberger, Lisa Marie, additional, Castro-Borges, William, additional, Rabello, Ana Lucia Teles, additional, Harn, Donald A., additional, Medeiros, Lia Carolina Soares, additional, Jeremias, Wander de Jesus, additional, Siqueira, Liliane Maria Vidal, additional, Pereira, Caroline Stephane Salviano, additional, Pedrosa, Maria Luysa Camargos, additional, Almeida, Nathalie Bonatti Franco, additional, Almeida, Aureo, additional, Lambertucci, Jose Roberto, additional, Carneiro, Nídia Francisca de Figueiredo, additional, Coelho, Paulo Marcos Zech, additional, and Grenfell, Rafaella Fortini Queiroz, additional
- Published
- 2019
- Full Text
- View/download PDF
6. Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
- Author
-
Portilho, Laysa Gomes, primary, Duarte, Bruna Custódio Dias, additional, Queiroz, Fábio Ribeiro, additional, Ribeiro, Thales Henrique Cherubino, additional, Jeremias, Wander de Jesus, additional, Babá, Elio Hideo, additional, Coelho, Paulo Marcos Zech, additional, Morais, Enyara Rezende, additional, Cabral, Fernanda Janku, additional, Caldeira, Roberta Lima, additional, and Gomes, Matheus de Souza, additional
- Published
- 2019
- Full Text
- View/download PDF
7. Investigation of the Larvicidal Effect of Guinea (Petiveria alliacea) on Larvae of Mosquitoes of the Species A. Aegypti
- Author
-
Hartmann, Ingrid, primary, da Silva, Andreia, additional, Walter, Maria Elena, additional, and Jeremias, Wander de Jesus, additional
- Published
- 2018
- Full Text
- View/download PDF
8. Whole genome analysis of a schistosomiasis-transmitting freshwater snail
- Author
-
Adema, Coen M., Hillier, Ladeana W., Jones, Catherine S., Loker, Eric S., Knight, Matty, Minx, Patrick, Oliveira, Guilherme, Raghavan, Nithya, Shedlock, Andrew, Do Amaral, Laurence Rodrigues, Arican-goktas, Halime D., Assis, Juliana G., Baba, Elio Hideo, Baron, Olga L., Bayne, Christopher J., Bickham-wright, Utibe, Biggar, Kyle K., Blouin, Michael, Bonning, Bryony C., Botka, Chris, Bridger, Joanna M., Buckley, Katherine M., Buddenborg, Sarah K., Caldeira, Roberta Lima, Carleton, Julia, Carvalho, Omar S., Castillo, Maria G., Chalmers, Iain W., Christensens, Mikkel, Clifton, Sandra, Cosseau, Celine, Coustau, Christine, Cripps, Richard M., Cuesta-astroz, Yesid, Cummins, Scott F., Di Stephano, Leon, Dinguirard, Nathalie, Duval, David, Emrich, Scott, Feschotte, Cedric, Feyereisen, Rene, Fitzgerald, Peter, Fronick, Catrina, Fulton, Lucinda, Galinier, Richard, Gava, Sandra G., Geusz, Michael, Geyer, Kathrin K., Giraldo-calderon, Gloria I., Gomes, Matheus De Souza, Gordy, Michelle A., Gourbal, Benjamin, Grunau, Christoph, Hanington, Patrick C., Hoffmann, Karl F., Hughes, Daniel, Humphries, Judith, Jackson, Daniel J., Jannotti-passos, Liana K., Jeremias, Wander De Jesus, Jobling, Susan, Kamel, Bishoy, Kapusta, Aurelie, Kaur, Satwant, Koene, Joris M., Kohn, Andrea B., Lawson, Dan, Lawton, Scott P., Liang, Di, Limpanont, Yanin, Liu, Sijun, Lockyer, Anne E., Lovato, Tyanna L., Ludolf, Fernanda, Magrini, Vince, Mcmanus, Donald P., Medina, Monica, Misra, Milind, Mitta, Guillaume, Mkoji, Gerald M., Montague, Michael J., Montelongo, Cesar, Moroz, Leonid L., Munoz-torres, Monica C., Niazi, Umar, Noble, Leslie R., Oliveira, Francislon S., Pais, Fabiano S., Papenfuss, Anthony T., Peace, Rob, Pena, Janeth J., Pila, Emmanuel A., Quelais, Titouan, Raney, Brian J., Rast, Jonathan P., Rollinson, David, Rosse, Izinara C., Rotgans, Bronwyn, Routledge, Edwin J., Ryan, Kathryn M., Scholte, Larissa L. S., Storey, Kenneth B., Swain, Martin, Tennessen, Jacob A., Tomlinson, Chad, Trujillo, Damian L., Volpi, Emanuela V., Walker, Anthony J., Wang, Tianfang, Wannaporn, Ittiprasert, Warren, Wesley C., Wu, Xiao-jun, Yoshino, Timothy P., Yusuf, Mohammed, Zhang, Si-ming, Zhao, Min, Wilson, Richard K., Adema, Coen M., Hillier, Ladeana W., Jones, Catherine S., Loker, Eric S., Knight, Matty, Minx, Patrick, Oliveira, Guilherme, Raghavan, Nithya, Shedlock, Andrew, Do Amaral, Laurence Rodrigues, Arican-goktas, Halime D., Assis, Juliana G., Baba, Elio Hideo, Baron, Olga L., Bayne, Christopher J., Bickham-wright, Utibe, Biggar, Kyle K., Blouin, Michael, Bonning, Bryony C., Botka, Chris, Bridger, Joanna M., Buckley, Katherine M., Buddenborg, Sarah K., Caldeira, Roberta Lima, Carleton, Julia, Carvalho, Omar S., Castillo, Maria G., Chalmers, Iain W., Christensens, Mikkel, Clifton, Sandra, Cosseau, Celine, Coustau, Christine, Cripps, Richard M., Cuesta-astroz, Yesid, Cummins, Scott F., Di Stephano, Leon, Dinguirard, Nathalie, Duval, David, Emrich, Scott, Feschotte, Cedric, Feyereisen, Rene, Fitzgerald, Peter, Fronick, Catrina, Fulton, Lucinda, Galinier, Richard, Gava, Sandra G., Geusz, Michael, Geyer, Kathrin K., Giraldo-calderon, Gloria I., Gomes, Matheus De Souza, Gordy, Michelle A., Gourbal, Benjamin, Grunau, Christoph, Hanington, Patrick C., Hoffmann, Karl F., Hughes, Daniel, Humphries, Judith, Jackson, Daniel J., Jannotti-passos, Liana K., Jeremias, Wander De Jesus, Jobling, Susan, Kamel, Bishoy, Kapusta, Aurelie, Kaur, Satwant, Koene, Joris M., Kohn, Andrea B., Lawson, Dan, Lawton, Scott P., Liang, Di, Limpanont, Yanin, Liu, Sijun, Lockyer, Anne E., Lovato, Tyanna L., Ludolf, Fernanda, Magrini, Vince, Mcmanus, Donald P., Medina, Monica, Misra, Milind, Mitta, Guillaume, Mkoji, Gerald M., Montague, Michael J., Montelongo, Cesar, Moroz, Leonid L., Munoz-torres, Monica C., Niazi, Umar, Noble, Leslie R., Oliveira, Francislon S., Pais, Fabiano S., Papenfuss, Anthony T., Peace, Rob, Pena, Janeth J., Pila, Emmanuel A., Quelais, Titouan, Raney, Brian J., Rast, Jonathan P., Rollinson, David, Rosse, Izinara C., Rotgans, Bronwyn, Routledge, Edwin J., Ryan, Kathryn M., Scholte, Larissa L. S., Storey, Kenneth B., Swain, Martin, Tennessen, Jacob A., Tomlinson, Chad, Trujillo, Damian L., Volpi, Emanuela V., Walker, Anthony J., Wang, Tianfang, Wannaporn, Ittiprasert, Warren, Wesley C., Wu, Xiao-jun, Yoshino, Timothy P., Yusuf, Mohammed, Zhang, Si-ming, Zhao, Min, and Wilson, Richard K.
- Abstract
Biomphalaria snails are instrumental in transmission of the human blood fluke Schistosoma mansoni. With the World Health Organization's goal to eliminate schistosomiasis as a global health problem by 2025, there is now renewed emphasis on snail control. Here, we characterize the genome of Biomphalaria glabrata, a lophotrochozoan protostome, and provide timely and important information on snail biology. We describe aspects of phero-perception, stress responses, immune function and regulation of gene expression that support the persistence of B. glabrata in the field and may define this species as a suitable snail host for S. mansoni. We identify several potential targets for developing novel control measures aimed at reducing snail-mediated transmission of schistosomiasis.
- Published
- 2017
- Full Text
- View/download PDF
9. Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
- Author
-
Queiroz, Fábio Ribeiro, primary, Silva, Luciana Maria, additional, Jeremias, Wander de Jesus, additional, Babá, Élio Hideo, additional, Caldeira, Roberta Lima, additional, Coelho, Paulo Marcos Zech, additional, and Gomes, Matheus de Souza, additional
- Published
- 2017
- Full Text
- View/download PDF
10. Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum
- Author
-
Jeremias, Wander de Jesus, primary, Araújo, Flávio Marcos Gomes, additional, Queiroz, Fábio Ribeiro, additional, Pais, Fabiano Sviatopolk Mirsky, additional, Mattos, Ana Carolina Alves de, additional, Salim, Anna Christina de Matos, additional, Coelho, Paulo Marcos Zech, additional, Oliveira, Guilherme Correa, additional, Kusel, John Robert, additional, Guerra-Sá, Renata, additional, Coimbra, Roney Santos, additional, and Babá, Élio Hideo, additional
- Published
- 2017
- Full Text
- View/download PDF
11. Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum.
- Author
-
Araújo, Flávio Marcos Gomes, Queiroz, Fábio Ribeiro, Pais, Fabiano Sviatopolk Mirsky, Mattos, Ana Carolina Alves de, Salim, Anna Christina de Matos, Coelho, Paulo Marcos Zech, Coimbra, Roney Santos, Babá, Élio Hideo, Jeremias, Wander de Jesus, Oliveira, Guilherme Correa, Kusel, John Robert, and Guerra-Sá, Renata
- Subjects
GENETIC regulation ,SEQUENCE analysis ,SCHISTOSOMA mansoni ,GENETICS ,PARASITES ,SKIN disease genetics ,GENETIC transcription ,POLYMERASE chain reaction ,MICROORGANISMS - Abstract
Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the host. Thus, it is very important for the parasite to remain within the skin after entering the host for a period of about 3 days, at which time it can then reach the venous system, migrate to the lungs and, by the end of eighth day post-infection, it reach the portal venous system, while undergoing minimal changes in morphology. However, after just a few days in the portal blood system, the parasite experiences an extraordinary increase in biomass and significant morphological alterations. Therefore, determining the constituents of the portal venous system that may trigger these changes that causes the parasite to consolidate its development inside the vertebrate host, thus causing the disease schistosomiasis, is essential. The present work simulated the conditions found in the portal venous system of the vertebrate host by exposing schistosomula of S. mansoni to in vitro culture in the presence of portal serum of the hamster, Mesocricetus auratus. Two different incubation periods were evaluated, one of 3 hours and one of 12 hours. These time periods were used to mimic the early contact of the parasite with portal serum during the course of natural infection. As a control, parasites were incubated in presence of hamster peripheral serum, in order to compare gene expression signatures between the two conditions. The mRNA obtained from parasites cultured under both conditions were submitted to a whole transcriptome library preparation and sequenced with a next generation platform. On average, nearly 15 million reads were produced per sample and, for the purpose of gene expression quantification, only reads mapped to one location of the transcriptome were considered. After statistical analysis, we found 103 genes differentially expressed by schistosomula cultured for 3 hours and 12 hours in the presence of hamster portal serum. After the subtraction of a second list of genes, also differentially expressed between schistosomula cultured for 3 hours and 12 hours in presence of peripheral serum, a set of 58 genes was finally established. This pattern was further validated for a subset of 17 genes, by measuring gene expression through quantitative real time polymerase chain reaction (qPCR). Processes that were activated by the portal serum stimulus include response to stress, membrane transport, protein synthesis and folding/degradation, signaling, cytoskeleton arrangement, cell adhesion and nucleotide synthesis. Additionally, a smaller number of genes down-regulated under the same condition act on cholinergic signaling, inorganic cation and organic anion membrane transport, cell adhesion and cytoskeleton arrangement. Considering the role of these genes in triggering processes that allow the parasite to quickly adapt, escape the immune response of the host and start maturation into an adult worm after contact with the portal serum, this work may point to unexplored molecular targets for drug discovery and vaccine development against schistosomiasis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
12. Localização física do BAC clone AL 619337 no genoma de Schistosoma mansoni utilizando a técnica de FISH (fluorescence in situ hybridization)
- Author
-
Jeremias, Wander de Jesus and Babá, Élio Hideo
- Subjects
Esquistossomose ,Schistosoma mansoni ,Técnica Fish ,Biologia molecular - Abstract
A Esquistossomose, também conhecida como “Bilharziose” ou, popularmente, “Barriga d’água” ou “Chistosa” é uma doença crônica e debilitante ou mesmo fatal em alguns casos, que afeta principalmente indivíduos em áreas rurais, sendo endêmica em países tropicais e subtropicais. È causada pelo platelminto trematódeo digenético Schistosoma mansoni. Devido a sua grande importância sócio-econômica da doença em países em desenvolvimento, foi montado um projeto para seqüenciamento e estudo do genoma de seu agente etiológico, o S. mansoni. A ênfase inicial do projeto foi dada na etapa de seqüenciamento de cDNA, devido à existência de poucos genes seqüenciados do parasita. Como parte deste projeto, iniciou-se a construção de um mapa físico do genoma do S. mansoni. E para a consolidação de tal mapa, a localização física de grandes fragmentos de DNA genômico clonados em vetores BAC e YAC no genoma do parasita por meio da técnica de FISH (“Fluorescence in situ Hybridization”) é de fundamental importância. Neste trabalho, o DNA do BAC clone AL619337, desde então denominado BAC2A, foi fisicamente localizado no genoma do S. mansoni por meio da técnica de FISH. Foi estabelecido como 28 dias o melhor tempo de infecção do caramujo hospedeiro para obtenção de maior número de células metafásicas do parasita por lâmina. Uma vez ajustada a concentração do fluoróforo PI com qual os cromossomos metafásicos do parasita foram contra corados e também do conjugado Avidina-FITC, o sinal de hibridização foi visualizado na região de transição entre eucromatina e heterocromatina do braço curto do cromossomo sexual W em todas as metáfases de fêmea observadas. Não foi observado sinal homólogo no outro cromossomo sexual Z, mostrando que o DNA localizado é específico de fêmeas de S. mansoni. Trabalhos desta natureza permitem obter informações sobre a estrutura do genoma. Tais informações associadas a outras referentes à função de determinado gene permitem responder questões determinantes na patologia causada por este importante parasita. The Schistosoma mansoni genome physical map is under construction and large DNA fragments localization by FISH is priority for this strategy. Therefore, little information about this respect is actually available. To contribute with this initiative, a BAC clone DNA was located on the S. mansoni genome by FISH technique in this work. The BAC DNA was labeled by the biotinylated 14-dCTP incorporation in a randomly primers amplification labeling system, using the Klenow polymerase. Cells on metaphase were recovered by dissection of the intramoluscan stage, secondary sporocyst. These were fixed and used to prepare slides containing metaphase spreads. The labeled BAC DNA was hybridized to the S. mansoni chromosomes and the slides were washed several times before to reveal the hybridization signal with an Avidin-FITC conjugated. Chromosomes were counter stained with Propidium Iodide in the anti fade solution, and the slides were covered and observed in an epi-fluorescent microscopy coupled to a digital camera to capture the images. C banding was performed to help in identifying and assembling the chromosomes. We were able to establish the better infection time of the snail that yielded good amounts of metaphase spreads. The PI and FITC fluorescence were adjusted too, according to the microscopy power of resolution.The main signal was observed in the heterochromatineuchromatin transition region of the chromosome W short arm in all female metaphases observed, but nether on the chromosome Z of the male metaphases, showing that the localized BAC clone contains a female specific DNA.With this work, we hope to contribute to the S. mansoni genome physical mapping, becoming an additional place to obtain DNA localization by FISH for this important worm. After the sequencing of the genome is finished, gaining knowledge of the sequences localization is an important step. It will help to associate the physical structure of genome and the functional data of the genes for better understanding different features related to the pathology caused for this important worm.
- Published
- 2004
13. The skin migratory stage of the schistosomulum of Schistosoma mansoni has a surface showing greater permeability and activity in membrane internalisation than other forms of skin or mechanical schistosomula.
- Author
-
JEREMIAS, WANDER DE JESUS, DA CUNHA MELO, JOSE RENAN, BABA, ELIO HIDEO, ZECH COELHO, PAULO MARCOS, and KUSEL, JOHN ROBERT
- Subjects
- *
SCHISTOSOMA mansoni , *MEMBRANE permeability (Biology) , *DEXTRAN , *SKIN permeability , *ENDOCYTOSIS , *BIOMARKERS , *IN vitro studies , *LABORATORY mice - Abstract
Skin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariae in vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariae in vitro and from mouse skin penetrated by cercariae in vivo. Schistosomula can also be produced from cercariae applied through a syringe or in a vortex. When certain surface properties of the different forms of schistosomula were compared, those migrating from mouse skin penetrated by cercariae in vivo or in vitro had greatly increased permeability to membrane impermeant molecules such as Lucifer yellow and high molecular weight dextrans. These migrating forms also possessed surfaces which showed greatly enhanced uptake into internal membrane vesicles of the dye FM 143, a marker for endocytosis. This greatly enhanced activity and permeability of the surfaces of tissue migrating schistosomula is likely to be of great importance in the adaptation to the new host. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
14. Caracterização de genes talin e os efeitos do silenciamento por RNAi usando miR-190 em Schistosoma mansoni
- Author
-
Paiva, Thales Henrique de, Cota, Renata Guerra de Sá, Jeremias, Wander de Jesus, and Borges, William de Castro
- Subjects
Proteínas ,Schistosoma mansoni ,Expressão gênica - Abstract
Programa de Pós-Graduação em Biotecnologia. Núcleo de Pesquisas em Ciências Biológicas, Pró-Reitoria de Pesquisa de Pós Graduação, Universidade Federal de Ouro Preto. A esquistossomose é uma doença parasitária de grande importância econômica e social afetando aproximadamente 200mi/ano de pessoas no mundo. Causada pelo nematódeo do gênero Schistosoma (especificamente neste estudo o S. mansoni por ser o de maior prevalência no Brasil), continua sendo um desafio para a comunidade científica sua compreensão ampla e descoberta de novas tecnologias diagnósticas e terapêuticas. Este estudo realizado trata da caracterização in vitro e in silico da proteína talin bem como avaliar os efeitos ultraestruturais após silenciamento in vitro por seu microRNA intrônico miR-190 em vermes adultos. As proteínas Talinas são uma família de proteínas grandes adaptadoras que conectam uma família de moléculas de adesão celular chamadas integrinas aos filamentos de F-actina do citoesqueleto. Há dois tipos de genes Talin em vertebrados. Talin 1 é essencial para o processo de adesão celular mediado por integrinas enquanto o papel do Talin 2 é desconhecido. Duas entradas da proteína talina previamente anotadas no genoma do S. mansoni, identificadas como Smp_167010 e Smp_142630 foram objeto desse estudo. Análises de predição estrutural in silico foram feitas e observado um padrão diferencial de domínios funcionais (PFAM), bem como alto grau de similaridade e identidade com seus ortólogos (SWISS-MODEL) e os dados indicam que as duas entradas não são oriundas de splicing alternativo e sim de diferenciação e especiação com valores de bootstrap próximo ou atingindo 100 (filogenia). Amostras de vermes adultos machos e fêmeas, cercárias, esquistossômulos mecanicamente transformados (EMT3,5h – EMT-24h) e ovos de S. mansoni foram utilizados para traçar o perfil de expressão gênica por qRT-PCR usando o gene constitutivo EIF4E como controle endógeno. A expressão gênica relativa foi determinada pelo método 2 –ΔCq. A análise estatística foi realizada utilizando o teste one-way ANOVA (Tukey) com P
- Published
- 2019
15. Immunogenicity and safety of inactivated SARS-CoV-2 vaccine (CoronaVac) using two-dose primary protocol in children and adolescents (Immunita-002, Brazil): A phase IV six-month follow up.
- Author
-
Corsini CA, Martins PFDS, Filgueiras PS, Lourenço AJ, Lima AES, Gomes SVC, Jeremias WJ, Alves PA, Fernandes GDR, Castro LLME, de Carvalho AT, Azevedo ACC, Curimbaba CAL, Lorencini DA, Junior EM, da Silva VM, Cervi MC, Borges MC, Nogueira ML, Campos GRF, Correa PRL, Carvalho TML, Dos Reis JGAC, Reis EVS, Castilho LDR, de Lima PR, do Nascimento JPR, de Oliveira JG, Filho OAM, and Grenfell RFQE
- Abstract
Introduction: Vaccines are essential for the prevention and control of several diseases, indeed, monitoring the immune response generated by vaccines is crucial. The immune response generated by vaccination against SARS-CoV-2 in children and adolescents is not well defined regarding to the intensity and medium to long-term duration of a protective immune response, which may point out the need of booster doses and might support the decisions in public health., Objective: The study aims to evaluate the immunogenicity and safety of inactivated SARS-CoV-2 vaccine (CoronaVac) in a two-dose primary protocol in children and adolescent aging from 3 to 17 years old in Brazil., Methods: Participants were invited to participate in the research at two public healthcare centers located in Serrana (São Paulo) and Belo Horizonte (Minas Gerais), Brazil. Participants underwent medical interviews to gather their medical history, including COVID-19 history and medical records. Physical exams were conducted, including weight, blood pressure, temperature, and pulse rate measurements. Blood samples were obtained from the participants before vaccination, 1 month after the first dose, and 1, 3, and 6 months after the second dose and were followed by a virtual platform for monitoring post-vaccination reactions and symptoms of COVID-19. SARS-CoV-2 genome from Swab samples of COVID-19 positive individuals were sequenced by NGS. Total antibodies were measured by ELISA and neutralizing antibodies to B.1 lineage and Omicron variant (BA.1) quantified by PRNT and VNT. The cellular immune response was evaluated by flow cytometry by the quantification of systemic soluble immune mediators., Results: The follow-up of 640 participants showed that the CoronaVac vaccine (Sinovac/Butantan Institute) was able to significantly induce the production of total IgG antibodies to SARS-CoV-2 and the production of neutralizing antibodies to B.1 lineage and Omicron variant. In addition, a robust cellular immune response was observed with wide release of pro-inflammatory and regulatory mediators in the early post-immunization moments. Adverse events recorded so far have been mild and transient except for seven serious adverse events reported on VigiMed., Conclusions: The results indicate a robust and sustained immune response induced by the CoronaVac vaccine in children and adolescents up to six months, providing evidences to support the safety and immunogenicity of this effective immunizer., Competing Interests: MLN has received research grants from Instituto Butantan, Janssen Vaccines and Prevention B.V., Medicago R&D Inc, and Pfizer/BioNTech SE. RFQG has received grants from Instituto Butantan.Additional Declarations: No competing interests reported.
- Published
- 2024
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.