Search

The aim of this study was to evaluate the association between prostate cancer (PCa) and Human papillomavirus (HPV) infection in the Mexican population. We studied 356 paraffin-embedded tissues from unrelated Mexican men with PCa or benign prostatic hyperplasia (BPH), with the latter serving as control. HPV detection was performed by polymerase chain reaction (PCR) using universal primers, and viral genotypes were detected using sequencing or multiplex PCR. Light microscopy analyses enabled the identification of koilocytes in samples subsequently analyzed for HPV detection by in situ PCR and for p16-INK4A expression by immunohistochemistry. The results showed that high risk- (HR) HPVs were detected in 37/189 (19.6%) PCa specimens compared to 16/167 (9.6%) of BHP specimens (odds ratio 2.3; 95% CI= 1.2 to 4.3; p=0.01). These data suggest HR-HPV may play a role in PCa. HPV 52 and 58 were the most frequent genotypes (33 and 17%, respectively) detected in the population studied. Koilocytes were detected in all in situ PCR-HPV-positive samples, representing a pathognomonic feature of infection, and we observed the overexpression of p16-INK4A in HPV-positive samples compared to HPV-negative samples, indirectly suggesting the presence of HR-HPV E7 oncoprotein. These results suggest that HPV infection plays an important role in prostate cancer development.

Breast cancer (BC) is the most frequent malignancy among women worldwide and has been associated with high mortality because of the late treatment of the disease. Our group has proposed a selective ablation of breast cancer cells by the use of magnetic fields assisted by magnetic nanoparticles. The principle is to increase the conductivity of tumoral tissue by the use of a bioconjugated "nanoparticle-antibody" that recognizes specific antigens on the surface of the cancer cells. The aim of this study was to evaluate the c-erbB-2 antigen in breast cancer cells of type BT-474, MCF-7, and MDA-MB-231 as a possible target for the use of magnetic nanoparticles coupled to a specific Monoclonal Antibody (Mab). Quantitative real-time polymerase chain reaction and flow cytometry were used to estimate the relative expressions of the c-erbB-2 gene and the c-erbB-2 antigen in the cell lines, respectively. A covalent union of magnetic nanoparticles to anti c-erbB-2 Mab was used to develop the bioconjugate. Fluorescence microscopy was used to determine the cells that were tagged by the bioconjugate. The results show a well-differentiated relative expression of c-erbB-2 in the studied cell lines and are qualitatively in agreement with the fluorescent marking by the magnetic nanoparticles. The selected breast cancer cells appear to be suitable for experimental evaluation of selective targeting by magnetic nanoparticles.

Breast cancer is the most frequent neoplasia in women worldwide. Our research group has proposed a new technology focused on the selective ablation of breast tumors by Radiofrequency (RF) assisted with Magnetic Nanoprobes. The Nanoprobes require specific antigens overexpressed in breast cancer as potential targets. The aim of this study was to evaluate the relative expression of genes erbB-3 and tgfb1 by RT Q-PCR in breast cancer tissue from an animal model as potential Nanoprobe targets. Cancer tissue was evaluated in early and late stages in order to identify a differentiated relative expression that may be used as therapeutic targets for the nanoprobe. The findings indicate that protein Tgfb1 represents a potential target for the nanoprobe in early stages of breast cancer.

To know if radiological relationship exists among the index corticodiaphyseal of Lizaur Utrilla (ICD) and the corticofemoral diaphyseal of Gómez García (ICDF) with the density bony (DMO) mineral column power station and hip for the diagnosis of osteoporosis; with the purpose of optimizing the simple X-ray of hip well during the valuation of the bony quality.One carries out a study of field, traverse, comparative, descriptive and observational, where you study a representative sample of 90 feminine patients divides in three equal groups: group 1:30 healthy (control) patients from 20 to 40 years of age; group 2: patient postmenopausal and group 3:30 patients with the diagnose of hip fracture; same that were subjected to studies of simple X-ray of hip including the femur proximal as well as central DMO, stops later to obtain those mentioned indexes before mentioned.For effects of this study decided to implement a new index on the base of the smallest trocanter, to which is assigned the name of index corticometaphyseal (ICM); hurtling the following results: the quantity of the bony mineral is bigger in the column (mediates: 0.973 +/- 0.168 g/cm2) that in the hip (mediates: 0.852 +/- 0.164 g/cm2); significant (p0.001) difference exists among each one of the indexes; the index that bigger (35 cases) correlation had with DMO was lCM with 75.5%, (68 cases) continued by ICD with 60% and (54 cases) for I finish ICDF 38%,Radiological relationship of 75.5% exists between IMC and central DMO of column and hip for the diagnosis of osteoporosis.