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A variety of genetic techniques have been devised to determine cell lineage relationships during tissue development. Some of these systems monitor cell lineages spatially and/or temporally without regard to gene expression by the cells, whereas others correlate gene expression with the lineage under study. The GAL4 Technique for Real-time and Clonal Expression (G-TRACE) system allows for rapid, fluorescent protein-based visualization of both current and past GAL4 expression patterns and is therefore amenable to genome-wide expression-based lineage screens. Here we describe the results from such a screen, performed by undergraduate students of the University of California, Los Angeles (UCLA) Undergraduate Research Consortium for Functional Genomics (URCFG) and high school summer scholars as part of a discovery-based education program. The results of the screen, which reveal novel expression-based lineage patterns within the brain, the imaginal disc epithelia, and the hematopoietic lymph gland, have been compiled into the G-TRACE Expression Database (GED), an online resource for use by the Drosophila research community. The impact of this discovery-based research experience on student learning gains was assessed independently and shown to be greater than that of similar programs conducted elsewhere. Furthermore, students participating in the URCFG showed considerably higher STEM retention rates than UCLA STEM students that did not participate in the URCFG, as well as STEM students nationwide.

Objetivo: Identificar contribuições de uma intervenção educacional no conhecimento de pacientes geriátricos sobre aspectos de segurança no tratamento com a varfarina. Métodos: Estudo de intervenção de caráter longitudinal conduzido em uma clínica de anticoagulação de um hospital público universitário localizado em Minas Gerais, realizado com pacientes geriátricos que possuíam diagnóstico de fibrilação atrial valvar ou não valvar em uso de varfarina. Os participantes responderam a um questionário sobre conhecimentos gerais da terapia anticoagulante com varfarina, validado no Brasil (OAK test) e analisado por meio de um comitê de juízes especialistas para seleção das questões sobre segurança desse instrumento no presente estudo. Posteriormente os pacientes participaram de uma intervenção educacional centrada no paciente e no autocuidado, o desfecho analisado foi a pontuação do teste de conhecimento sobre anticoagulação antes (T0), imediatamente após (T1) e seis meses após (T2) a intervenção. Os dados foram analisados de forma descritiva por meio de frequências absolutas e relativas. Os dados foram analisados de forma descritiva por meio de frequências absolutas e relativas. Resultados: 43 pacientes com idade média de 71±7,6 anos, com predominância do sexo feminino 25; 58,1% e média de escolaridade de 5±4,8 anos participaram do estudo. As questões analisadas pelos juízes 1, 2, 3, 4, 5, 6, 9, 12, 13, 15, 17, 19 e 20 tiveram IVC avaliado em 1,0, as questões 7, 10, 16 e 18 tiveram IVC avaliado em 0,92, as questões 8 e 14 tiveram IVC abaixo de 0,78 e foram avaliados em 0,75 e 0,58 respectivamente, sendo assim excluídas do estudo. Para a maioria das questões identificou-se melhoria no conhecimento dos pacientes sobre segurança no tratamento após a intervenção educacional (P 0,75, segundo avaliação os juízes.

Objective: To characterize patients with insulin-dependent Diabetes Mellitus (DM) accompanied by a PF service in a teaching hospital, as well as to identify the occurrence of hospital readmissions and associated factors. Methods: This is a cross-sectional study carried out in a general teaching hospital, being included patients above 18 years old, of both genders, with type 1 or 2 DM diagnosis were admitted from April 2015 to April 2016, accompanied by the Pharmaceutical Follow-up Service of the hospital, who used insulin during hospitalization and received indication for using this drug in the hospital discharge prescription. Data were collected through consultation in electronic medical records and telephone contact after the hospital discharge. Data were recorded in a Microsoft Excel spreadsheet, with the patients subdivided into groups with and without readmission, followed by univariate analysis of associated factors by means of proportion comparison and logistic model with penalized likelihood. Results: Patients who were re-admitted had higher blood sugar values, fewer pharmaceutical interventions regarding insulin use, and longer average hospitalization time. The analysis of the association between variables and readmission did not present statistical significance. Conclusions: The study points out to weaknesses related to the offer of care to the patients under study, such as weaknesses in the coverage for offering actions related to health education in the periods before and after hospital discharge, difficulty in accessing the specialist physician and non-achievement of blood sugar measurement by some patients. There was no association between any of the analyzed variables and hospital readmission.