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Background Antibody-driven complement system (CS) activation has been associated with protection against symptomatic dengue virus (DENV) infection. Aggregation, opsonization, lysis, and phagocytosis are mechanisms triggered by antibody-antigen immunocomplexes following fixation of the component 1q (C1q) and activation of the classical pathway. As a result, DENV neutralization and clearance are facilitated, whereas antibody-dependent enhancement of infection is inhibited. We investigated the ability of antibodies produced in response to Takeda's dengue vaccine candidate, TAK-003, to fix C1q and activate CS. Methods Serum samples were collected from seronegative and seropositive participants in a phase 2 clinical trial (DEN-203), pre- and postvaccination. Samples were evaluated for the presence of complement-fixing antibodies (CFAs) against DENV using a Luminex multiplex-based immunoassay. Results TAK-003 elicited production of CFAs against all 4 DENV serotypes, which persisted for 1 year postvaccination, irrespective of baseline serostatus. CFA levels were correlated with neutralizing antibody titers and virus-binding total IgG and IgG1 concentrations. Furthermore, efficiency of CFA fixation was greater in samples with higher polyclonal IgG avidity. Conclusions These results indicate that antibodies produced after TAK-003 vaccination are functional in both activating CS and neutralizing virus infection by all DENV serotypes, which may contribute to efficacy of TAK-003. Clinical Trials Registration NCT01511250.

The study of environmental perception of a population is important to understand their interrelationships with environment. In this context, the study aimed to analyze the environmental sense and its social representation as teachers and students from senior high school in Timor-Leste and Paraiba. The questionnaire method was used as a data collection instrument applied to teachers and students for qualitative analysis which characterize as a detailed accepting of significance and characteristics of a situation demonstrated by interviewees. The researcher pursues to understand phenomena due to social performers perspective of studied situation and traces her analysis about phenomena studied. As was used features of phenomenological research which is concerned with essentials study (perception and consciousness). By collected data analysis used questionnaires filled through teachers and students of Timor-Leste and Paraiba was verified that environmental awareness in these schools find in fragmented plan when do not reserve implications for the present and future. So as a result, the author point out the relevance of pedagogical practice especially in schools of Timor Leste which assist social personals to merge their knowledge to unified understanding about environment correlations. Environmental perception creates opportunities for reflection and sensibility of social personals concerning the environment conservation. New understanding perspectives about environment approaches from those who share a new way of thinking to act and being in flagrant divergence with anthropocentric tradition, new social consciousness from environmental education practice should consider the social representations. Thus, this study aims to contribute both to strengthen the schools of Timor Leste on perception and awareness of the environment. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES O estudo de Percepção Ambiental de uma população é fundamental para compreender as inter-relações da mesma com o seu ambiente. Dentro desse contexto este trabalho teve como principal objetivo analisar a percepção ambiental e as representações sociais de meio ambiente de professores e educandos do Ensino Médio de escolas públicas do Timor-Leste e Paraíba. Foi utilizado o questionário como um instrumento de coleta de dados aplicados aos professores e educandos, pressupostos da pesquisa qualitativa a qual se caracteriza como uma compreensão detalhada dos significados e características de uma situação apresentada pelos entrevistados. O pesquisador busca entender os fenômenos segundo a perspectiva dos atores sociais da situação estudadas e a partir daí situa sua interpretação dos fenômenos estudados. Assim como se utilizou elementos da Pesquisa Fenomenológica a qual se preocupa com o estudo das essências (Percepção e consciência). A partir da análise dos dados coletados nos questionários preenchidos pelos professores e educandos de Timor-Leste e Paraíba verificou-se que a percepção ambiental destas escolas encontra-se num plano fragmentado, quando não distante das implicações para o presente e para o futuro. Portanto, como resultado o autor aponta a relevância de uma prática pedagógica principalmente nas escolas de Timor-Leste que auxilie os agentes sociais a aglutinarem seus conhecimentos para uma compreensão mais unificada de todas as relações que compõem o meio ambiente. Acreditou-se que a percepção ambiental cria oportunidades à reflexão e sensibilização dos atores sociais no que tange à conservação do meio ambiente. Novas perspectivas de entendimento a respeito do Meio Ambiente provêm do campo daqueles que compartilham um novo modo de pensar, de agir e de ser, em flagrante discordância com tradição antropocêntrica, uma nova consciência social a partir da prática da Educação Ambiental será mais abrangente se considerar as representações sociais. Desta forma essa pesquisa tem a finalidade de contribuir tanto de fortalecer as escolas de Timor-Leste sobre a percepção e a consciência do meio ambiente.