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14. Genome-wide analysis of long non-coding RNAs (lncRNAs) in tea plants (Camellia sinensis) lateral roots in response to nitrogen application.

Author

Shunkai Hu, Yimeng Hu, Huiling Mei, Jianjie Li, Wei Xuan, Jeyaraj, Anburaj, Zhen Zhao, Yuxin Zhao, Rui Han, Xuan Chen, and Xinghui Li

Subjects
LINCRNA, TEA, NUCLEOTIDE sequencing, NITROGEN, PLANT hormones, GREENHOUSES
Abstract

Tea (Camellia sinensis) is one of the significant cash crops in China. As a leaf crop, nitrogen supply can not only increase the number of new shoots and leaves but also improve the tenderness of the former. However, a conundrum remains in science, which is the molecular mechanism of nitrogen use efficiency, especially long noncoding RNA (lncRNA). In this study, a total of 16,452 lncRNAs were identified through high-throughput sequencing analysis of lateral roots under nitrogen stress and control conditions, of which 9,451 were differentially expressed lncRNAs (DE-lncRNAs). To figure out the potential function of nitrogenresponsive lncRNAs, co-expression clustering was employed between lncRNAs and coding genes. KEGG enrichment analysis revealed nitrogen-responsive lncRNAs may involve in many biological processes such as plant hormone signal transduction, nitrogen metabolism and protein processing in endoplasmic reticulum. The expression abundance of 12 DE-lncRNAs were further verified by RT-PCR, and their expression trends were consistent with the results of RNA-seq. This study expands the research on lncRNAs in tea plants, provides a novel perspective for the potential regulation of lncRNAs on nitrogen stress, and valuable resources for further improving the nitrogen use efficiency of tea plants. [ABSTRACT FROM AUTHOR]

Published
2023
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17. Glutamine Synthetases Play a Vital Role in High Accumulation of Theanine in Tender Shoots of Albino Tea Germplasm “Huabai 1”

Author

Yu, Ying, primary, Kou, Xiaobing, additional, Gao, Ruoshi, additional, Chen, Xuefei, additional, Zhao, Zhen, additional, Mei, Huiling, additional, Li, Jianjie, additional, Jeyaraj, Anburaj, additional, Thangaraj, Kuberan, additional, Periakaruppan, Rajiv, additional, Zhuang, Jing, additional, Chen, Xuan, additional, Arkorful, Emmanuel, additional, and Li, Xinghui, additional

Published
2021
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19. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil

Author

Fu, Haiping, primary, Li, Huan, additional, Yin, Peng, additional, Mei, Huiling, additional, Li, Jianjie, additional, Zhou, Pinqian, additional, Wang, Yuanjiang, additional, Ma, Qingping, additional, Jeyaraj, Anburaj, additional, Thangaraj, Kuberan, additional, Chen, Xuan, additional, Li, Xinghui, additional, and Guo, Guiyi, additional

Published
2021
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23. Untargeted metabolomic analysis using UPLC-MS/MS identifies metabolites involved in shoot growth and development in pruned tea plants (Camellia sinensis (L.) O. Kuntz)

Author

Arkorful, Emmanuel, primary, Yu, Ying, additional, Chen, Changsong, additional, Lu, Li, additional, Hu, Shunkai, additional, Yu, Hanpu, additional, Ma, Qingping, additional, Thangaraj, Kuberan, additional, Periakaruppan, Rajiv, additional, Jeyaraj, Anburaj, additional, Chen, Xuan, additional, and Li, Xinghui, additional

Published
2020
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24. A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze]

Author

Author), Shunkai Hu(Former Corresponding, primary, Zhang, Mi, additional, Yang, Yiqing, additional, Xuan, Wei, additional, Zou, Zhongwei, additional, Arkorful, Emmanuel, additional, Chen, Yi, additional, Ma, Qingping, additional, Jeyaraj, Anburaj, additional, Chen, Xuan, additional, and Author), Xinghui Li(New Corresponding, additional

Published
2020
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31. Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [Camellia sinensis(L.) O. Kuntze]

Author

Jeyaraj, Anburaj, Elango, Tamilselvi, Li, Xinghui, and Guo, Guiyi

Abstract

ABSTRACTMicroRNAs play a central role in responses to biotic stressors through their interactions with their target mRNAs. Tea plant (Camellia sinensisL.), an important beverage crop, is vulnerable to tea geometrid and anthracnose disease that causes considerable crop loss and tea production worldwide. Sustainable production of tea in the current scenario to biotic factors is major challenges. To overcome the problem of biotic stresses, high-throughput sequencing (HTS) with bioinformatics analyses has been used as an effective approach for the identification of stress-responsive miRNAs and their regulatory functions in tea plant. These stress-responsive miRNAs can be utilized for miRNA-mediated gene silencing to enhance stress tolerance in tea plant. Therefore, this review summarizes the current understanding of miRNAs regulatory functions in tea plant responding to Ectropis obliqueand Colletotrichum gloeosporioidesattacks for future miRNA research. Also, it highlights the utilization of miRNA-mediated gene silencing strategies for developing biotic stress-tolerant tea plant.

Published
2020
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33. Metabolic Regulation Profiling of Carbon and Nitrogen in Tea Plants [Camellia sinensis(L.) O. Kuntze] in Response to Shading

Author

Li, Yuchen, Jeyaraj, Anburaj, Yu, Hanpu, Wang, Yu, Ma, Qingping, Chen, Xuan, Sun, Haiwei, Zhang, Hong, Ding, Zhaotang, and Li, Xinghui

Abstract

Manipulating light transmission by shading is the most effective method of improving the nutritional value and sensory qualities of tea. In this study, the metabolic profiling of two tea cultivars (“Yulv” and “Maotouzhong”) in response to different shading periods during the summer season was performed using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS) and gas chromatography-mass spectrometry (GC-MS). The metabolic pathway analyses showed that the glycolytic pathway and the tricarboxylic acid cycle (TCA cycle) in the leaves and shoots of “Maotouzhong” were significantly inhibited by long-term shading. The nitrogen metabolism in the leaves of the two cultivars was promoted by short-term shading, while it was inhibited by long-term shading. However, the nitrogen metabolism in the shoots of the two cultivars was always inhibited by shading, whether for short or long-term periods. In addition, the intensity of the flavonoid metabolism in both tea cultivars could be reduced by shading. These results revealed that shading could regulate the carbon and nitrogen metabolism and short-term shading could improve the tea quality to some extent.

Published
2020
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34. Identification and validation of sugarcane streak mosaic virus-encoded microRNAs and their targets in sugarcane

Author

Chandran Viswanathan, Gajjeraman Prabu, and Jeyaraj Anburaj

Subjects
Chlorophyll, Viral pathogenesis, Molecular Sequence Data, Plant Science, Genome, Viral, Genome, Virus, RNA interference, Gene Expression Regulation, Plant, Gene, Phylogeny, Plant Diseases, Regulation of gene expression, Genetics, biology, Base Sequence, Potyviridae, Reverse Transcriptase Polymerase Chain Reaction, RNA, General Medicine, biology.organism_classification, Virology, Carotenoids, Saccharum, MicroRNAs, Gene Ontology, Host-Pathogen Interactions, Nucleic Acid Conformation, RNA, Viral, Agronomy and Crop Science, Sequence Alignment
Abstract

Identification of miRNAs encoded by sugarcane streak mosaic virus and understanding their host target genes might be used as a new strategy to study viral pathogenesis and controlling mosaic disease in sugarcane. Plants have developed several defense mechanisms to cope with various pathogens (bacteria, fungi, virus, and phytoplasma). Among these, RNA interference (RNAi)-mediated defense against viral infection was found to be a major innate immune response. As a counter attack strategy against the host defense, viruses produce suppressors of host RNAi pathway. MicroRNAs (miRNAs) are an abundant class of short (~18–22 nucleotide) non-coding single-stranded RNAs involved in RNAi pathway leading to post-transcriptional regulation of gene expression. Sugarcane streak mosaic virus (SCSMV) is a distinct strain of Potyviridae family which has a single-stranded positive-sense RNA genome causing mosaic disease in sugarcane. In this study, we computationally predicted and experimentally validated the miRNA encoded by the SCSMV genome with detection efficiency of 99.9 % in stem-loop RT-qPCR and predicted their potential gene targets in sugarcane. These sugarcane target genes considerably broaden future investigation of the SCSMV-encoded miRNA function during viral pathogenesis and might be applied as a new strategy for controlling mosaic disease in sugarcane.

Published
2013

35. Absolute quantification of microRNAs in green tea ( Camellia sinensis) by stem-loop quantitative real-time PCR.

Author

Hou, Ying‐Hui, Jeyaraj, Anburaj, Zhang, Xiao, and Wei, Chao‐Ling

Subjects
*MICRORNA, *GREEN tea, *HAIRPIN (Genetics), *POLYMERASE chain reaction, *HYPOTHESIS
Abstract

BACKGROUND There are some studies to show that food-derived plant microRNAs ( miRNAs) may be detected in mammals. The research evidence has provoked a considerable debate whether plant-derived miRNAs exert the same regulatory functions as endogenous animal miRNAs. To test the hypothesis, methods of highly sensitive absolute quantification miRNAs have been developed. However, absolute miRNA quantification of green tea has not yet been reported. This study is the first to build an absolute quantification method to detect miRNAs level in green tea using stem-loop quantitative real-time PCR ( qRT-PCR). RESULTS Two miRNAs, csn- miR164 (a conserved miRNA) and csn- miRn329 (a tea-specific miRNA), were selected as examples for the detection and absolute quantification of miRNAs in green tea samples using stem-loop qRT-PCR. The content of csn- miR164 was significantly higher in the Yuexi Cuilan ( YX) samples than in the Shucheng Orchid ( SC) samples. The content of csn- miRn329 was found to be high at the start of processing in leaf tissues in both the withering and soaking experiments, after which it gradually decreased with time. CONCLUSION To the best of our knowledge, this is the first report to absolutely quantify the miRNAs present in green tea. This method will help to further investigate the possibility that tea-derived miRNAs may play an important role on defending against various diseases in humans. © 2016 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2017
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36. Snakebite and Its Socio-Economic Impact on the Rural Population of Tamil Nadu, India

Author

Vaiyapuri, Sakthivel, primary, Vaiyapuri, Rajendran, additional, Ashokan, Rajesh, additional, Ramasamy, Karthikeyan, additional, Nattamaisundar, Kameshwaran, additional, Jeyaraj, Anburaj, additional, Chandran, Viswanathan, additional, Gajjeraman, Prabu, additional, Baksh, M. Fazil, additional, Gibbins, Jonathan M., additional, and Hutchinson, E. Gail, additional

Published
2013
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37. Immunomodulatory Effect of Methotrexate Abruptly Controls Keratinocyte Activation in Psoriasis

Author

Elango, Tamilselvi, Zhang, Xuejun, Gnanaraj, Pushpa, Li, Xinghui, Dayalan, Haripriya, and Jeyaraj, Anburaj

Subjects
Medical
Abstract

In psoriatic skin, epidermal keratinocytes (KCs) undergo deregulated inflammatory response that leads to prolonged expression of inflammatory mediators as well as abnormal keratins. Due to immune and genetic factors, KCs get activated and cell balance gets disturbed. This activation is mainly due to deregulated inflammatory response. A vicious cycle of KC-immune response called KC activation cycle leads to psoriasis. In psoriatic skin, epidermal KCs undergo deregulated inflammatory response that leads to prolonged expression of inflammatory mediators as well as abnormal keratins. Methotrexate (MTX) an immunosuppressive agent has been used as a standard drug to treat severe psoriasis. Acanthosis and abnormal terminal differentiation was mainly due to the mutation in epidermal keratins. In turn, disease severity and relapsing of psoriasis are mainly due to the mutation of hyperproliferative keratins. These novel keratin mutations in psoriatic epidermis might be one of the causative factors for psoriasis. MTX strongly regulates the KC activation cycle by deregulated inflammatory markers and maintains normal keratin phenotype on hyperproliferating KC, thereby controlling acanthosis in psoriasis patients.

Published
2022

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