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Criteria used for evaluating drought tolerance of wheat cultivars demanding more time and efforts are usually not efficient and conclusive. Present study was conducted to evaluate ABA sensitivity as a criterion for evaluating drought tolerance of wheat cultivars at an early stage. Ten cultivars of wheat were subjected to drought at 3-leaf stage to select the most sensitive and two tolerant cultivars using mortality rate. Tatara was found the most susceptible whereas GA-2002 and Chakwal-50 were the cultivars with maximum drought tolerance. These cultivars were used to study sensitivity to applied absicic acid (based on germination index and relative growth inhibition rate) and physiological responses (leaf water content, chlorophyll stability index, coefficient of relative inhibition and proline accumulation) under drought stress. Highest ABA-sensitivity was recorded in GA-2002 and Chakwal-50 whereas Tatara manifested minimum sensitivity. ABA-sensitivity corresponded to physiological indices of drought tolerance. Results show that ABA-sensitivity is an efficient criterion that can be used to evaluate drought tolerance of wheat cultivars at early stage. [ABSTRACT FROM AUTHOR]

Background Aims and methods Results Conclusions The application of nanoparticles (NPs) in horticultural crops is in a tremendous increase. NPs help in the overcoming of stresses with positive impacts on plant growth and development. Silver NPs (AgNPs) have numerous pre‐ and postharvest applications in agriculture.This study aimed to evaluate the effect of AgNPs application (0, 50, 100, 150, and 200 ppm) at three spray intervals (5, 10, and 15 days) on the morphological and compositional traits, and defense system of strawberry.Results showed that AgNPs application enhanced the growth, yield, quality, and nutritional aspects of strawberry grown under field conditions. Shoot fresh weight (20.20 g) and leaf number/plant (41.53) were enhanced at 100 ppm AgNPs and 15 days interval. A stunted growth of strawberry plants was recorded at 200 ppm AgNPs. Moreover, a 15‐day‐spray interval was found optimum for the improvement of major morphological traits. Fruit size, yield, total soluble solids, acidity, and antioxidant capacity were improved at 50 and 100 ppm AgNPs. The activation of plant defense system, that is, superoxide dismutase, peroxidase, catalase, total soluble protein, and ascorbic acid was improved under AgNPs foliar application. The activation of stress indicating marker malondialdehyde outlined a high defense response of strawberry at 150 ppm AgNPs.Conclusively, AgNPs application at 50, 100, and 150 ppm can be considered effective for sustainable strawberry production. [ABSTRACT FROM AUTHOR]

Production and utilization of nanoparticles (NPs) are increasing due to their positive and stimulating effects on biological systems. Silver (Ag) NPs improve seed germination, photosynthetic efficiency, plant growth, and antimicrobial activities. In this study, the effects of chemo-blended Ag NPs on wheat were investigated using the gel-free/label-free proteomic technique. Morphological analysis revealed that chemo-blended Ag NPs resulted in the increase of shoot length, shoot fresh weight, root length, and root fresh weight. Proteomic analysis indicated that proteins related to photosynthesis and protein synthesis were increased, while glycolysis, signaling, and cell wall related proteins were decreased. Proteins related to redox and mitochondrial electron transport chain were also decreased. Glycolysis associated proteins such as glyceraldehyde-3-phosphate dehydrogenase increased as well as decreased, while phosphoenol pyruvate carboxylase was decreased. Antioxidant enzyme activities such as superoxide dismutase, catalase, and peroxidase were promoted in response to the chemo-blended Ag NPs. These results suggested that chemo-blended Ag NPs promoted plant growth and development through regulation of energy metabolism by suppression of glycolysis. Number of grains/spike, 100-grains weight, and yield of wheat were stimulated with chemo-blended Ag NPs. Morphological study of next generational wheat plants depicted normal growth, and no toxic effects were observed. Therefore, morphological, proteomic, yield, and next generation results revealed that chemo-blended Ag NPs may promote plant growth and development through alteration in plant metabolism. [ABSTRACT FROM AUTHOR]