42 results on '"Ji Hyun-Ju"'
Search Results
2. Supplementary Figure 4 from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Figure 4 - PDF file 1098K, KRT19 inhibition does not down-regulate PTEN protein level through modulation of PTEN stability
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- 2023
3. Supplementary Figure 6 from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Figure 6 - PDF file 1339K, Statistical analyses of western blots and RT-PCR data
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- 2023
4. Supplementary Information from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Information - PDF file 163K, Legends to Supplementary data and Materials and Methods
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- 2023
5. Supplementary Fig. 7B from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Fig. 7A - PDF file 188K, Correlation between KRT19 and HER2 expression in breast cancer patient samples
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- 2023
6. Data from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Purpose: Keratin19 (KRT19) is the smallest known type I intermediate filament and is used as a marker for reverse transcriptase PCR–mediated detection of disseminated tumors. In this study, we investigated the functional analysis of KRT19 in human breast cancer.Experimental Design: Using a short hairpin RNA system, we silenced KRT19 in breast cancer cells. KRT19 silencing was verified by Western blot analysis and immunocytochemistry. We further examined the effect of KRT19 silencing on breast cancer cells by cell proliferation, migration, invasion, colony formation assay, cell-cycle analysis, immunocytochemistry, immunohistochemistry, and mouse xenograft assay.Results: Silencing of KRT19 resulted in increased cell proliferation, migration, invasion, and survival. These effects were mediated by upregulation of Akt signaling as a result of reduced PTEN mRNA expression. Silencing of KRT19 decreased the nuclear import of early growth response-1 (Egr1), a transcriptional factor for PTEN transcription, through reduced association between Egr1 and importin-7. We also confirmed that silencing of KRT19 increased tumor formation in a xenograft model.Conclusions: KRT19 is a potential tumor suppressor that negatively regulates Akt signaling through modulation of Egr1 nuclear localization. Clin Cancer Res; 19(16); 4335–46. ©2013 AACR.
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- 2023
7. Supplementary Figure 5 from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Figure 5 - PDF file 2097K, KRT19 regulated nuclear localization of Egr1 transcriptional factor
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- 2023
8. Supplementary Fig. 3 from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Fig. 3 - PDF file 2033K, KRT19 inhibition induces Akt activity through down-regulation of PTEN expression.
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- 2023
9. Supplementary Fig. 2 from Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Incheol Shin, In-Sun Chu, Myung Chan Gye, Soon Young Shin, Sarah Shim, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Wonseok Yang, and Ji-hyun Ju
- Abstract
Supplementary Fig. 2 - PDF file 2822K, KRT19 inhibition up-regulates colony formation, cell migration and invasion
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- 2023
10. Silencing of CD133 inhibits GLUT1‐mediated glucose transport through downregulation of the HER3/Akt/mTOR pathway in colon cancer
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Hyungjoo Kim, Seogho Son, Incheol Shin, and Ji-hyun Ju
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Receptor, ErbB-3 ,Biophysics ,Biological Transport, Active ,Down-Regulation ,medicine.disease_cause ,Biochemistry ,03 medical and health sciences ,Downregulation and upregulation ,Structural Biology ,Cancer stem cell ,Genetics ,medicine ,Humans ,Gene silencing ,AC133 Antigen ,neoplasms ,Molecular Biology ,Protein kinase B ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,Glucose Transporter Type 1 ,0303 health sciences ,biology ,Chemistry ,TOR Serine-Threonine Kinases ,030302 biochemistry & molecular biology ,Glucose transporter ,Cell Biology ,HCT116 Cells ,Gene Expression Regulation, Neoplastic ,carbohydrates (lipids) ,Glucose ,Colonic Neoplasms ,embryonic structures ,biology.protein ,Cancer research ,GLUT1 ,Carcinogenesis ,HT29 Cells ,Proto-Oncogene Proteins c-akt - Abstract
Cluster of differentiation 133 (CD133) is a transmembrane glycoprotein that has been reported as a marker of cancer stem cells or cancer-initiating cells in various cancers. However, its contribution to tumorigenesis and differentiation remains to be elucidated. To determine the role of CD133 in colon cancer, we silenced CD133 in human colon cancer cells. Silencing of CD133 results in decreased cell proliferation, survival, migration, invasion, and glucose transport. These effects are mediated by downregulation of the human epidermal growth factor receptor 3 (HER3)/Akt/mTOR signaling pathway, culminating in reduced expression of the glucose transporter GLUT1. We also confirm that the cellular phenotypes of CD133-silenced cells are mediated by GLUT1 downregulation. We conclude that CD133 is a potential tumor initiator that positively regulates GLUT1 expression through modulation of HER3/Akt/mTOR signaling.
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- 2020
11. Morphometry of Spinal Nerve Composition and Thicknesses of Lumbar Plexus Nerves for Use in Clinical Applications
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Ji Hyun-Ju and Hur Mi-Sun
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Spinal nerve composition ,Anatomy ,Lumbar plexus ,Thickness - Abstract
SUMMARY: The aim of this study was to clarify the diverse spinal compositions of the branches of the lumbar plexus in terms of their prevalence rates and thicknesses. Thirty lumbar plexuses extracted from Korean adults were used in this study. The nerve fascicles were separated and traced with the aid of a surgical microscope. The thickness of each spinal nerve component was calculated based on the mean of the largest and smallest diameters using digital calipers under the surgical microscope. The most common patterns of the spinal composition of the branches of the lumbar plexus were as follows: The iliohypogastric nerve (IHN) and the ilioinguinal nerve (IIN) arose from the ventral ramus of the first lumbar nerve (L1), the genitofemoral nerve (GFN) arose from the anterior division of the ventral ramus of the second lumbar nerve (L2), and the lateral femoral cutaneous nerve (LFCN) arose from the posterior division of the ventral ramus of theL2, the femoral nerve (FN) arose from the posterior division of the ventral ramus of L2-the fourth lumbar nerve (L4), with the thickest spinal component derived from the third lumbar nerve (L3), and the obturator nerve (OBN) arose from the anterior division of the ventral ramus of L2-L4, with the thickest spinal component derived from L3. However, when L5 constituted the FN and OBN, the thickest spinal components of the FN and OBN was L4. This morphometric study has measured the thicknesses of diverse spinal components that constitute the branches of the lumbar plexus after separating the nerve fascicles. The thicknesses of the various spinal components of these branches can be compared in order to understand which make the main and minor contributions to the lower limb.
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- 2021
12. EGFR negates the proliferative effect of oncogenic HER2 in MDA-MB-231 cells
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Kyungmin Lee, Wonseok Yang, KeeSoo Nam, Incheol Shin, Ji-hyun Ju, and Sunhwa Oh
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medicine.medical_specialty ,Biophysics ,Breast Neoplasms ,Biochemistry ,Normal cell ,Cell Line, Tumor ,Internal medicine ,medicine ,Humans ,skin and connective tissue diseases ,neoplasms ,Molecular Biology ,Triple-negative breast cancer ,Cell Proliferation ,DNA Primers ,Mda mb 231 ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,Chemistry ,Genes, erbB-2 ,Ligand (biochemistry) ,ErbB Receptors ,Endocrinology ,Cell culture ,Cancer research ,Female ,Breast cancer cells ,Tyrosine kinase ,EGFR Family - Abstract
Members of the EGFR family are potent mediators of normal cell growth and development. HER2 possesses an active tyrosine kinase domain, but no direct ligand has been identified. To investigate the differential effect of HER2 in breast cell lines, HER2 was overexpressed in MCF-10A, MCF7 and MDA-MB-231 cells. HER2 overexpression promoted proliferation, survival and migration in MCF-10A and MCF-7 cells. No significant differences were seen in proliferation, survival or migration between MDA-MB-231 vec and HER2 cells. The activity of downstream HER2 proteins increased in MCF-10A HER2 and MCF-7 HER2 cells but not in MDA-MB-231 HER2 cells. Exogenously expressed HER2 failed to associate with EGFR or HER3 in MDA-MB-231 cells, while overexpression of HER2 enhanced HER family dimerization in MCF-10A and MCF-7 cells.
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- 2015
13. An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses
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Chul Geun Kim, Ji Sook Kim, Ji-Hyun Ju, Yunho Gwak, EonSeon Jin, Jaekyung Hyun, Yew Lee, Woongsic Jung, and Chihong Song
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Models, Molecular ,Protein Folding ,In silico ,Biology ,Biochemistry ,Chloroplast membrane ,Protein Structure, Secondary ,law.invention ,Western blot ,Stress, Physiological ,Antifreeze protein ,law ,Antifreeze Proteins ,Botany ,Microalgae ,Genetics ,medicine ,neoplasms ,Molecular Biology ,Binding Sites ,medicine.diagnostic_test ,Ice ,digestive, oral, and skin physiology ,Mutagenesis ,Immunogold labelling ,Recombinant Proteins ,digestive system diseases ,embryonic structures ,Mutagenesis, Site-Directed ,Recombinant DNA ,Crystallization ,Intracellular ,Biotechnology - Abstract
The structure and function of the Antarctic marine diatom Chaetoceros neogracile antifreeze protein (Cn-AFP), as well as its expression levels and characteristics of the ice-binding site, were analyzed in the present study. In silico analysis revealed that the Cn-AFP promoter contains both light- and temperature-responsive elements. Northern and Western blot analyses demonstrated that both Cn-AFP transcript and protein expression were strongly and rapidly stimulated by freezing, as well as temperature and high light stress. Immunogold labeling revealed that Cn-AFP is preferentially localized to the intracellular space near the chloroplast membrane. Recombinant Cn-AFP had clear antifreeze activity. Protein-folding simulation was used to predict the putative ice-binding sites in Cn-AFP, and site-directed mutagenesis of the Cn-AFP b-face confirmed their identification.
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- 2014
14. Topographical study of the trapezius muscle, greater occipital nerve, and occipital artery for facilitating blockade of the greater occipital nerve
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Won, Hyung-Jin, primary, Ji, Hyun-Ju, additional, Song, Jae Kyeong, additional, Kim, Yeon-Dong, additional, and Won, Hyung-Sun, additional
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- 2018
- Full Text
- View/download PDF
15. Regulation of Cell Proliferation and Migration by Keratin19-Induced Nuclear Import of Early Growth Response-1 in Breast Cancer Cells
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Soon Young Shin, KeeSoo Nam, Myung Chan Gye, Kyungmin Lee, Wonseok Yang, Incheol Shin, Sarah Shim, Sunhwa Oh, Ji-hyun Ju, and In-Sun Chu
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Cancer Research ,Cell Survival ,Receptors, Cytoplasmic and Nuclear ,Breast Neoplasms ,Karyopherins ,Small hairpin RNA ,Mice ,Downregulation and upregulation ,Cell Movement ,Cell Line, Tumor ,Animals ,Humans ,PTEN ,Gene silencing ,Gene Silencing ,Protein kinase B ,Cell Proliferation ,Early Growth Response Protein 1 ,Cell Nucleus ,Keratin-19 ,Regulation of gene expression ,biology ,Cell growth ,PTEN Phosphohydrolase ,Molecular biology ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,Protein Transport ,Cell Transformation, Neoplastic ,Oncology ,biology.protein ,Cancer research ,Heterografts ,Female ,Signal transduction ,Proto-Oncogene Proteins c-akt ,Protein Binding ,Signal Transduction - Abstract
Purpose: Keratin19 (KRT19) is the smallest known type I intermediate filament and is used as a marker for reverse transcriptase PCR–mediated detection of disseminated tumors. In this study, we investigated the functional analysis of KRT19 in human breast cancer. Experimental Design: Using a short hairpin RNA system, we silenced KRT19 in breast cancer cells. KRT19 silencing was verified by Western blot analysis and immunocytochemistry. We further examined the effect of KRT19 silencing on breast cancer cells by cell proliferation, migration, invasion, colony formation assay, cell-cycle analysis, immunocytochemistry, immunohistochemistry, and mouse xenograft assay. Results: Silencing of KRT19 resulted in increased cell proliferation, migration, invasion, and survival. These effects were mediated by upregulation of Akt signaling as a result of reduced PTEN mRNA expression. Silencing of KRT19 decreased the nuclear import of early growth response-1 (Egr1), a transcriptional factor for PTEN transcription, through reduced association between Egr1 and importin-7. We also confirmed that silencing of KRT19 increased tumor formation in a xenograft model. Conclusions: KRT19 is a potential tumor suppressor that negatively regulates Akt signaling through modulation of Egr1 nuclear localization. Clin Cancer Res; 19(16); 4335–46. ©2013 AACR.
- Published
- 2013
16. Protein kinase Cδ negatively regulates Notch1-dependent transcription via a kinase-independent mechanism in vitro
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Sunhwa Oh, Minsoon Kim, Chul Geun Kim, Jieun Song, Kibeom Jang, Ji Hyun Ju, and Incheol Shin
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Proteasome Endopeptidase Complex ,Notch ,Transcription, Genetic ,Cell Survival ,Down-Regulation ,Biology ,Histone Deacetylases ,Cell Line ,Mice ,Protein Kinase Cδ ,Genes, Reporter ,Transcription (biology) ,Animals ,Humans ,Gene silencing ,Gene Silencing ,Receptor, Notch1 ,HES1 ,Kinase activity ,Promoter Regions, Genetic ,Protein kinase A ,Molecular Biology ,Cell Proliferation ,Promoter ,Cell Biology ,Molecular biology ,Protein Kinase C-delta ,Gene Expression Regulation ,Proto-Oncogene Proteins c-akt ,Transcription ,Chromatin immunoprecipitation ,Nuclear localization sequence - Abstract
Protein kinase Cδ (PKCδ) plays a significant role in the regulation of growth, apoptosis, and differentiation in a diversity of cell types. We investigated the effect of PKCδ on Notch1 intracellular domain (NICD)-mediated transcription with Notch transcription reporter constructs. The results indicate that co-expression of PKCδ down-regulated NICD-dependent transcription. Co-expression of a dominant negative PKCδ (K376R) variant lacking kinase activity was also able to downregulate NICD-dependent transcription, suggesting that PKCδ exerts its inhibitory effect via a kinase-independent mechanism(s). Interestingly, expression of PKCδ as well as K376R induced NICD up-regulation by inhibiting proteasome-mediated degradation of NICD, indicating that NICD protein quantity is not proportional to its transcriptional activity. When the subcellular distribution of NICD was investigated by both subcellular fractionation and immunocytochemistry, it was found that PKCδ and K376R effectively impaired proper nuclear localization of NICD, possibly via a physical association between NICD and PKCδ, which was confirmed by co-immunoprecipitation experiments. Chromatin immunoprecipitation assays revealed that both PKCδ and K376R inhibit the association of NICD with the promoter region of its target gene, Hes1. Furthermore, silencing of PKCδ resulted in increased NICD nuclear localization and NICD transcriptional activity in MCF-7 cells. PKCδ silencing-induced increase in anti-apoptotic survivin could not rescue apoptosis induced by doxorubicin. The data herein indicate that PKCδ can induce down-regulation of NICD transcriptional activity via a kinase-independent inhibition of NICD nuclear targeting and dissociation of NICD from target gene promoters.
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- 2012
17. CD24 enhances DNA damage-induced apoptosis by modulating NF-κB signaling in CD44-expressing breast cancer cells
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Incheol Shin, Kibeom Jang, Ji Hyun Ju, Jae Youn Yi, Minsoon Kim, Kyungmin Lee, Jongbin Kim, and Dong Young Noh
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Cancer Research ,DNA damage ,Blotting, Western ,Apoptosis ,Breast Neoplasms ,Cell Line, Tumor ,Radiation, Ionizing ,Tumor Cells, Cultured ,Humans ,Neoplasm Invasiveness ,RNA, Messenger ,RNA, Small Interfering ,Extracellular Signal-Regulated MAP Kinases ,Luciferases ,skin and connective tissue diseases ,Protein kinase A ,Cell Proliferation ,Antibiotics, Antineoplastic ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Kinase ,Cell growth ,Chemistry ,Cell Cycle ,CD44 ,HEK 293 cells ,NF-kappa B ,CD24 Antigen ,Cell Differentiation ,General Medicine ,Flow Cytometry ,Cell biology ,Doxorubicin ,biology.protein ,Female ,Mitogen-Activated Protein Kinases ,Signal transduction ,DNA Damage ,Signal Transduction - Abstract
Cluster of differentiation 24 (CD24) is a small glycosylphosphatidylinositol-linked cell surface molecule that is expressed in a variety of human carcinomas, including breast cancer. To determine the role of CD24 in breast cancer cells, we expressed CD24 in CD24-negative/low and cluster of differentiation 44 (CD44)-positive MDA-MB-231 metastatic breast cancer cells. Forced expression of CD24 resulted in a decrease in c-Raf/mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK)/mitogen-activated protein kinase signaling and reduced cell proliferation. Apoptosis induced by DNA damage was greatly enhanced in MDA-MB-231 CD24 cells as compared with MDA-MB-231 vec cells. CD24 expression efficiently attenuated DNA damage-induced nuclear factor-kappaB (NF-κB) signaling in MDA-MB-231 cells. However, in CD24-positive and CD44-negative/low MCF-7 cells, knockdown of CD24 did not significantly affect DNA damage-induced apoptosis nor NF-κB signaling. Silencing of CD24 in CD24/CD44-double-positive MDA-MB-468 cells partially rescued DNA damage-induced apoptosis. Transient transfection studies with 293T cells also revealed that CD24 attenuated cell viability and NF-κB signaling only when CD44 was cotransfected. These data indicate that CD24 expression potentiated DNA-induced apoptosis by suppressing antiapoptotic NF-κB signaling in CD44-expressing cells.
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- 2011
18. Induction of apoptotic cell death by phytoestrogens by up-regulating the levels of phospho-p53 and p21 in normal and malignant estrogen receptor α–negative breast cells
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Hye Sook Seo, Ji-hyun Ju, Kibeom Jang, and Incheol Shin
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Cyclin-Dependent Kinase Inhibitor p21 ,G2 Phase ,endocrine system ,Cell signaling ,Cell division ,Endocrinology, Diabetes and Metabolism ,Estrogen receptor ,Genistein ,Apoptosis ,Breast Neoplasms ,Phytoestrogens ,Biology ,chemistry.chemical_compound ,Endocrinology ,Cyclin D1 ,Cell Line, Tumor ,Humans ,Breast ,Apigenin ,skin and connective tissue diseases ,Cyclin B1 ,Cyclin-dependent kinase 1 ,Nutrition and Dietetics ,urogenital system ,Estrogen Receptor alpha ,food and beverages ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,chemistry ,Cancer research ,Female ,Quercetin ,Tumor Suppressor Protein p53 ,Cell Division - Abstract
In this study, we investigated the underlying mechanism by which phytoestrogens suppress the growth of normal (MCF-10A) and malignant (MDA-MB-231) estrogen receptor α (ERα)-negative breast cells. We hypothesized that phytoestrogen inhibits the proliferation of ERα-negative breast cancer cells. We found that all tested phytoestrogens (genistein, apigenin, and quercetin) suppressed the growth of both MCF-10A and MDA-MB-231 cells, as revealed by proliferation assays. These results were accompanied by an increase in the sub-G0/G1 apoptotic fractions as well as an increase in the cell population in the G2/M phase in both cell types, as revealed by cell cycle analysis. When we assessed the effect of phytoestrogens on the level of intracellular signaling molecules by Western blot analysis, we found that phytoestrogens increased the level of active p53 (phospho-p53) without changing the p53 level in both MCF-10A and MDA-MB-231 cells. Phytoestrogens also induced an increase in p21, a p53 target gene, and a decrease in either Bcl-xL or cyclin B1 in both cell types. In contrast, the protein levels of phosphatase and tensin homolog, cyclin D1, cell division control protein 2 homolog, phospho-cell division control protein 2 homolog, and p27 were not changed after phytoestrogen treatment. Our data indicate that phytoestrogens induce apoptotic cell death of ERα-negative breast cancer cells via p53-dependent pathway and suggest that phytoestrogens may be promising agents in the treatment and prevention of ERα-negative breast cancer.
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- 2011
19. Akt isoform-specific inhibition of MDA-MB-231 cell proliferation
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Kyungmin Lee, Wonseok Yang, Ji-hyun Ju, and Incheol Shin
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Cyclin E ,AKT1 ,Breast Neoplasms ,AKT2 ,Cell Line, Tumor ,Humans ,Protein Isoforms ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,biology ,Cell growth ,Chemistry ,Cyclin-Dependent Kinase 2 ,Cyclin-dependent kinase 2 ,Cell Biology ,Cell cycle ,Protein Structure, Tertiary ,Cell biology ,Proto-Oncogene Proteins c-raf ,embryonic structures ,Cancer research ,biology.protein ,Female ,Proto-Oncogene Proteins c-akt ,Cyclin-Dependent Kinase Inhibitor p27 ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction - Abstract
To dissect the isoform-specific roles of Akt in breast cancer cells, constitutively active Akt isoforms were introduced into MDA-MB-231 cells. Both Akt1 and Akt2 efficiently inhibited the growth of MDA-MB-231 cells. Overexpression of Akt1 down-regulated ERK activity inhibiting Ser 259 phosphorylation of c-Raf and subsequent downstream signaling. Akt2 overexpression up-regulated the cell cycle inhibitor p27. Cycloheximide decay assays showed that Akt2 increased the stability and nuclear localization of p27, thus inhibiting the cyclin E/CDK2 complex. These results suggest that the inhibition of cell proliferation by Akt1 and Akt2 is mediated by isoform-specific mechanisms.
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- 2011
20. S100A4 negatively regulates β-catenin by inducing the Egr-1-PTEN-Akt-GSK3β degradation pathway
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Incheol Shin, Kyungmin Lee, Ji-hyun Ju, Sunhwa Oh, Wonseok Yang, and KeeSoo Nam
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Cell signaling ,Proteasome Endopeptidase Complex ,Transcription, Genetic ,Dishevelled Proteins ,Down-Regulation ,Biology ,Glycogen Synthase Kinase 3 ,GSK-3 ,Cell Movement ,Cell Line, Tumor ,Tensin ,PTEN ,Humans ,S100 Calcium-Binding Protein A4 ,Phosphorylation ,RNA, Small Interfering ,Protein kinase B ,beta Catenin ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Early Growth Response Protein 1 ,Cell Nucleus ,Glycogen Synthase Kinase 3 beta ,S100 Proteins ,PTEN Phosphohydrolase ,Cell Biology ,Phosphoproteins ,HEK293 Cells ,Cell culture ,Catenin ,Cancer research ,biology.protein ,MCF-7 Cells ,RNA Interference ,Lithium Chloride ,Proto-Oncogene Proteins c-akt ,Protein Binding ,Signal Transduction - Abstract
S100A4, also known as the mts1 gene, has been reported as an invasive and metastatic marker for many types of cancers. S100A4 interacts with various target genes that affect tumor cell metastasis; however, little is known about cellular signaling pathways elicited by S100A4. In the current study, we demonstrate an inhibitory effect of S100A4 on β-catenin signaling in breast cancer cells. By overexpressing S100A4 in MCF-7, MDA-MB-231 and MDA-MB-453 breast cancer cells, we observed the down-regulation of β-catenin expression and β-catenin-dependent TCF/LEF transcriptional activities. The activity of GSK3β, which phosphorylates β-catenin and induces proteasomal degradation of β-catenin, was increased in S100A4-overexpressing cell lines. Blocking Glycogen Synthase Kinase (GSK3β) activity by lithium chloride or Dvl gene overexpression restored β-catenin expression. We also found that increased GSK3β activity was due to decrease in Akt activity resulting from Egr-1-induced phosphatase and tensin homolog (PTEN) expression. S100A4 induced Egr-1 nuclear localization by increasing the association between Egr-1 and importin-7 and this effect was reduced in S100A4 mutants that harbored a defect in nuclear localization signals. Collectively, we verify herein that S100A4 may act as a tumor suppressor in breast cancers by down-regulating the central signaling axis for tumor cell survival.
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- 2014
21. HER2 stabilizes survivin while concomitantly down-regulating survivin gene transcription by suppressing Notch cleavage
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Sunhwa Oh, Kee Soo Nam, Dong-Young Noh, Ji Hyun Ju, Wonseok Yang, Kyungmin Lee, and Incheol Shin
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MAPK/ERK pathway ,Transcription, Genetic ,MAP Kinase Signaling System ,Receptor, ErbB-2 ,Survivin ,Ubiquitin-Protein Ligases ,Notch signaling pathway ,Down-Regulation ,Breast Neoplasms ,X-Linked Inhibitor of Apoptosis Protein ,Biology ,Inhibitor of apoptosis ,Biochemistry ,Receptor tyrosine kinase ,Inhibitor of Apoptosis Proteins ,CDC2 Protein Kinase ,Chlorocebus aethiops ,Animals ,Humans ,skin and connective tissue diseases ,neoplasms ,Molecular Biology ,Protein kinase B ,Adaptor Proteins, Signal Transducing ,Cyclin-dependent kinase 1 ,Receptors, Notch ,Protein Stability ,Intracellular Signaling Peptides and Proteins ,Ubiquitination ,Cell Biology ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Notch proteins ,COS Cells ,Proteolysis ,Cancer research ,biology.protein ,Female ,Apoptosis Regulatory Proteins ,Proto-Oncogene Proteins c-akt ,HeLa Cells - Abstract
In breast cancer, the HER2 (human epidermal growth factor receptor 2) receptor tyrosine kinase is associated with extremely poor prognosis and survival. Notch signalling has a key role in cell-fate decisions, especially in cancer-initiating cells. The Notch intracellular domain produced by Notch cleavage is translocated to the nucleus where it activates transcription of target genes. To determine the combinatory effect of HER2 and Notch signalling in breast cancer, we investigated the effect of HER2 on Notch-induced cellular phenomena. We found the down-regulation of Notch-dependent transcriptional activity by HER2 overexpression. Also, the HER2/ERK (extracellular-signal-regulated kinase) signal pathway down-regulated the activity of γ-secretase. When we examined the protein level of Notch target genes in HER2-overexpressing cells, we observed that the level of survivin, downstream of Notch, increased in HER2 cells. We found that activation of ERK resulted in a decrease in XAF1 [XIAP (X-linked inhibitor of apoptosis)-associated factor 1] which reduced the formation of the XIAP–XAF1 E3 ligase complex to ubiquitinate survivin. In addition, Thr34 of survivin was shown to be the most important residue in determining survivin stability upon phosphorylation after HER2/Akt/CDK1 (cyclin-dependent kinase 1)–cyclin B1 signalling. The results of the present study show the combinatorial effects of HER2 and Notch during breast oncogenesis.
- Published
- 2013
22. Phosphorylation of p90RSK is associated with increased response to neoadjuvant chemotherapy in ER-positive breast cancer
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Ji-hyun Ju, Kyung Min Lee, Hee-Chul Shin, Hee Sung Kim, Jae Kyo Yi, Wonshik Han, Minju Yi, Dong-Young Noh, Hyeong-Gon Moon, Incheol Shin, Hea Young Lee, and Soo-Kyung Ahn
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Oncology ,MAPK/ERK pathway ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Predictive marker ,Estrogen receptor ,Breast Neoplasms ,lcsh:RC254-282 ,Ribosomal Protein S6 Kinases, 90-kDa ,Breast cancer ,Surgical oncology ,Internal medicine ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,Genetics ,medicine ,Humans ,Chemotherapy ,Doxorubicin ,Anthracyclines ,Phosphorylation ,Neoadjuvant therapy ,business.industry ,Middle Aged ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Neoadjuvant Therapy ,ERK ,Receptors, Estrogen ,Cancer research ,MCF-7 Cells ,Female ,Taxoids ,P90RSK ,business ,Estrogenreceptor ,medicine.drug ,Signal Transduction ,Research Article - Abstract
Background The clinical implication of Ras/Raf/ERK pathway activity in breast cancer tissue and its association with response to chemotherapy is controversial. We aimed to explore the value of p90RSK phosphorylation, a downstram molecule of the pathway, in predicting chemotherapy response in breast cancer. Methods The expression of phosphorylated p90RSK (phospho-p90RSK) and chemotherapy response was measured in 11 breast cancer cell lines and 21 breast cancer tissues. The predictive value of phospho-p90RSK was validated in core needle biopsy specimens of 112 locally advanced breast cancer patients who received anthracycline and taxane-based neoadjuvant chemotherapy. Results In 11 breast cancer cell lines, the relative expression of phospho-p90RSK was inversely correlated with cell survival after doxorubicin treatment (p = 0.021). Similar association was observed in fresh tissues from 21 breast cancer patients in terms of clinical response. In paraffin-embedded, formalin-fixed tissues from core needle biopsy tissues from 112 patients, positive phospho-p90RSK expression was associated with greater tumor shrinkage and smaller post-chemotherapy tumor size. The association between phospho-p90RSK expression and chemotherapy response was more evident in estrogen receptor(ER)-positive tumors. The expression of phosphor-p90RSK did not show a significant relationship with the incidence of pCR. P90RSK silencing using siRNA did not affect the cancer cell’s response to doxorubicin, and the expression of phospho-p90RSK was highly correlated with other Ras/Raf/ERK pathway activation. Conclusion Our results suggest that phospho-p90RSK expression, which reflects the tumor’s Ras/Raf/ERK/p90RSK pathway activation can be a potential predictive marker for chemotherapy response in ER-positive breast cancer which needs further independent validation.
- Published
- 2012
23. Glycogen synthase kinase 3-β phosphorylates novel S/T-P-S/T domains in Notch1 intracellular domain and induces its nuclear localization
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Ji Hyun Ju, Xiangzi Han, and Incheol Shin
- Subjects
Cytoplasm ,Transcription, Genetic ,Mutant ,Nuclear Localization Signals ,Biophysics ,Active Transport, Cell Nucleus ,Biology ,Biochemistry ,Glycogen Synthase Kinase 3 ,Mice ,GSK-3 ,Chlorocebus aethiops ,medicine ,NLS ,Animals ,Humans ,Phosphorylation ,Receptor, Notch1 ,Molecular Biology ,GSK3B ,Cell Nucleus ,COS cells ,Glycogen Synthase Kinase 3 beta ,Cell Biology ,Fusion protein ,Molecular biology ,Protein Structure, Tertiary ,Up-Regulation ,Cell nucleus ,medicine.anatomical_structure ,HEK293 Cells ,Amino Acid Substitution ,COS Cells ,Nuclear localization sequence - Abstract
We identified two S/T-P-S/T domains (2122-2124, 2126-2128) inducing Notch intracellular domain (NICD) nuclear localization. The GFP-NICD (1963-2145) fusion protein deletion mutant without classical NLS was localized in the nucleus like the full length GFP-NICD. However, quadruple substitution mutant (T2122A T2124A S2126A T2128A) showed increased cytoplasmic localization. GSK-3β enhanced nuclear localization and transcriptional activity of WT NICD but not of quadruple substitution mutant. In vitro kinase assays revealed that GSK-3β phosphorylated S and T residues in NICD S/T-P-S/T domains. These results suggest that the novel S/T-P-S/T domain, phosphorylated by GSK-3β is also involved in the nuclear localization of NICD as well as classical NLS.
- Published
- 2012
24. CD24 regulates cell proliferation and transforming growth factor β-induced epithelial to mesenchymal transition through modulation of integrin β1 stability
- Author
-
Wonseok Yang, Dong-Young Noh, Kyungmin Lee, Jae Youn Yi, Incheol Shin, Kibeom Jang, and Ji Hyun Ju
- Subjects
MAPK/ERK pathway ,Epithelial-Mesenchymal Transition ,Integrin ,Down-Regulation ,Small hairpin RNA ,Transforming Growth Factor beta1 ,Humans ,Epithelial–mesenchymal transition ,Phosphorylation ,RNA, Small Interfering ,skin and connective tissue diseases ,Cell adhesion ,Extracellular Signal-Regulated MAP Kinases ,Cell Proliferation ,Mitogen-Activated Protein Kinase Kinases ,biology ,Cell growth ,Chemistry ,Protein Stability ,Integrin beta1 ,CD24 Antigen ,Cell Biology ,G1 Phase Cell Cycle Checkpoints ,Cell biology ,HEK293 Cells ,Focal Adhesion Kinase 1 ,Cancer cell ,Cancer research ,biology.protein ,MCF-7 Cells ,RNA Interference ,raf Kinases ,Proto-oncogene tyrosine-protein kinase Src ,Protein Binding ,Signal Transduction - Abstract
To determine the role of CD24 in breast cancer cells, we knocked down CD24 in MCF-7 human breast cancer cells by retroviral delivery of shRNA. MCF-7 cells with knocked down CD24 (MCF-7 hCD24 shRNA) exhibited decreased cell proliferation and cell adhesion as compared to control MCF-7 mCD24 shRNA cells. Decreased proliferation of MCF-7 hCD24 shRNA cells resulted from the inhibition of cell cycle progression from G1 to S phase. The specific inhibition of MEK/ERK signaling by CD24 ablation might be responsible for the inhibition of cell proliferation. Phosphorylation of Src/FAK and TGF-β1-mediated epithelial to mesenchymal transition was also down-regulated in MCF-7 hCD24 shRNA cells. Reduced Src/FAK activity was caused by a decrease in integrin β1 bound with CD24 and subsequent destabilization of integrin β1. Our results suggest that down-regulation of Raf/MEK/ERK signaling via Src/FAK may be dependent on integrin β1 function and that this mechanism is largely responsible for the CD24 ablation-induced decreases in cell proliferation and epithelial to mesenchymal transition.
- Published
- 2012
25. Induction of apoptotic cell death by Pharbitis nil extract in HER2-overexpressing MCF-7 cells
- Author
-
Hye Sook Seo, Ji-hyun Ju, Incheol Shin, Min Jeong Jeon, Wonseok Yang, and Kyungmin Lee
- Subjects
MAPK/ERK pathway ,Cell signaling ,Receptor, ErbB-2 ,Cyclin D ,Apoptosis ,Breast Neoplasms ,chemistry.chemical_compound ,Cell Line, Tumor ,Drug Discovery ,Medicine ,Humans ,skin and connective tissue diseases ,Extracellular Signal-Regulated MAP Kinases ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Pharmacology ,Ipomoea nil ,biology ,business.industry ,Asia, Eastern ,Plant Extracts ,Cell Cycle ,Cell cycle ,Antineoplastic Agents, Phytogenic ,Cell biology ,chemistry ,Cell culture ,Immunology ,biology.protein ,Female ,Growth inhibition ,business ,Proto-Oncogene Proteins c-akt ,Phytotherapy - Abstract
Aim of the study We performed this study to investigate the anti-cancer activity of Pharbitis nil (PN) ethanol extract which has been used for herbal medicinal treatment against diseases in East Asia. Materials and methods We analyzed the effects of PN extract on proliferation of breast cancer cell lines, MCF-7 control vector (vec) and MCF-7 human epidermal growth factor receptor 2 (HER2) cells engineered to overexpress oncogenic HER2 via retroviral infection. We performed the proliferation assay to measure the growth rate of the cells. FACS analysis was used to analyze the cell cycle. Western blot analysis was used to investigate the effect of PN on the level and activation of intracellular molecules. Results We found that PN extract inhibited the proliferation of both MCF-7 vec and MCF-7 HER2 cells. This growth inhibition was accompanied with the increase of sub G0/G1 apoptotic fractions. When we check the efficiency of PN on the level of intracellular signaling molecules, we found that PN extract induced the inhibition of phosphorylation of HER2 and its downstream effectors, Akt and extracellular signal-regulated kinases (ERK). Active forms of both Akt and ERK were gradually decreased in PN-treated MCF-7 vec and MCF-7 HER2 cells suggesting that the growth suppressive activity of PN is related to signaling pathway. The level of cyclin D also diminished in PN-treated both cells suggesting that PN may inhibit the growth of MCF-7 vec and MCF-7 HER2 cells by perturbing cell cycle progression. It should be noted that PN decreased the growth rate of both MCF-7 vec and MCF-7 HER2 cells without changing the level and activation of p53. Conclusion PN extract suppressed the proliferation rate of HER-2 overexpressing MCF-7 breast cancer cells inducing apoptotic cell death in vitro . Our data demonstrates that PN extracts contain useful anti-tumor activity especially against HER2 overexpressing breast cancer.
- Published
- 2010
26. Danshen (Salvia miltiorrhiza) extract inhibits proliferation of breast cancer cells via modulation of Akt activity and p27 level
- Author
-
Ji-hyun Ju, Wonseok Yang, Incheol Shin, Xiangzi Han, and Min Jeong Jeon
- Subjects
Receptor, ErbB-2 ,Breast Neoplasms ,Salvia miltiorrhiza ,Pharmacology ,Receptor tyrosine kinase ,Cell Line, Tumor ,Humans ,Medicine ,Phosphorylation ,skin and connective tissue diseases ,Protein kinase B ,Cell Proliferation ,biology ,Traditional medicine ,Cell growth ,business.industry ,Cell Cycle ,Cell cycle ,Cell culture ,biology.protein ,Female ,Signal transduction ,business ,Proto-Oncogene Proteins c-akt ,Cyclin-Dependent Kinase Inhibitor p27 ,Drugs, Chinese Herbal ,Phenanthrolines - Abstract
Danshen is widely used in traditional Chinese medicine, often in combination with other herbs. To check the effect of Danshen on the proliferation of breast cancer cells, Danshen extract was used to treat MCF-7 and MCF-7 HER2 cells, the latter of which overexpresses HER2. HER2 is a receptor tyrosine kinase, and is involved in signal transduction pathways leading to tumor cell proliferation. MTT and cell proliferation assays revealed that Danshen strongly inhibited the proliferation of both MCF-7 vec cells and MCF-7 HER2 cells. Flow cytometry analyses indicated that Danshen induced cell cycle delay in the G1 phase. HER2 expression was shown to confer resistance to Danshen-induced inhibition of proliferation and cell cycle delay, suggesting that HER2 is responsible for the resistance to Danshen. Danshen treatment induced the down-regulation of Akt phosphorylation and an increase in p27 in MCF-7 vec and MCF-7 HER2 cells. Nevertheless, MCF-7 HER2 cells were more resistant to the Danshen-induced inhibition of Akt phosphorylation and p27 up-regulation.
- Published
- 2009
27. Protein expression profiling of primary mammary epithelial cells derived from MMTV-neumice revealed that HER2/NEU-driven changes in protein expression are functionally clustered
- Author
-
Taehoon Lee, Kyungmin Lee, Dong-Young Noh, Jaeyoon Kim, Sungwoo Park, Incheol Shin, and Ji Hyun Ju
- Subjects
Proteomics ,Genetically modified mouse ,Genotype ,Receptor, ErbB-2 ,Blotting, Western ,Clinical Biochemistry ,Cell ,Protein Array Analysis ,Apoptosis ,Mice, Transgenic ,Biology ,medicine.disease_cause ,Biochemistry ,Mass Spectrometry ,HER2/neu ,Mice ,Downregulation and upregulation ,Cell Line, Tumor ,Cell Adhesion ,Genetics ,medicine ,Animals ,Humans ,Mammary Glands, Human ,Molecular Biology ,Wild type ,Epithelial Cells ,Heterozygote advantage ,Cell Biology ,Molecular biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Mammary Tumor Virus, Mouse ,Proteome ,biology.protein ,Carcinogenesis - Abstract
MMTV-neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV-neu heterozygote mice and wild type (WT) littermates using two-dimensional digest (ChemDigest™/Trypsin)-LC-MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti-apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion-related proteins were downregulated in PMECs from MMTV-neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU-driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010
- Published
- 2009
28. Phase II trial of S-1 in combination with gemcitabine for chemo-naïve patients with locally advanced or metastatic pancreatic cancer
- Author
-
Chi-Young Jeong, Kim Seok Hyun, Hyun Jin Kim, Jung Hun Kang, Hye Jung Kim, Ji-Hyun Ju, Tae Hyo Kim, Gyeong-Won Lee, and Hoon Gu Kim
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Pancreatic disease ,Neutropenia ,Toxicology ,Gastroenterology ,Deoxycytidine ,Internal medicine ,Pancreatic cancer ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Pharmacology (medical) ,Neoplasm Metastasis ,Aged ,Tegafur ,Pharmacology ,Performance status ,business.industry ,Cancer ,Combination chemotherapy ,Middle Aged ,medicine.disease ,Survival Analysis ,Gemcitabine ,Surgery ,Pancreatic Neoplasms ,Drug Combinations ,Oxonic Acid ,Oncology ,Female ,business ,Progressive disease ,medicine.drug - Abstract
We performed a phase II study of combination chemotherapy with S-1 plus gemcitabine for treating chemo-naïve patients with unresectable pancreatic cancer to evaluate the efficacy and toxicity.Patients with histologically confirmed unresectable pancreatic cancer were eligible. The treatment consisted of S-1 (40 mg/m(2) p.o. b.i.d. from D1 to 14) and gemcitabine (1,250 mg/m(2) on D1 and 8), repeated every 3 weeks.Thirty-two patients were enrolled between March 2005 and December 2007. No complete response was observed and a partial response was observed in 14 patients (44.0%), stable disease in eight patients (25.0%), and progressive disease in eight patients (25.0%). The median time to progression was 4.92 months (95% CI: 4.16-5.67 months), and the median overall survival was 7.89 months (95% CI: 5.96-9.82 months). The survival duration was significantly longer for the patients with a good performance status compared with that of the patients with a poor performance status. The major toxicities were grade 3-4 neutropenia (9, 28.1%), grade 3/4 thrombocytopenia (5, 15.6%), and grade 3 diarrhea (5, 15.6%).The combination chemotherapy of S-1 and gemcitabine showed promising antitumor activity and manageable toxicities, and especially for the good performance status patients with unresectable pancreatic cancer.
- Published
- 2008
29. A Case of Liver Abscess due to Sump Syndrome and Spontaneous Drainage to the Stomach
- Author
-
Chang Yoon Ha, Ok Jae Lee, Woon Tae Jung, Hyun Ju Min, Yeon Jeong Ahn, Ji Hyun Ju, Eun Young Yun, Tae Hyo Kim, Jae Jin Hwang, Hyun Jin Kim, Hyun Jeong Jang, and Sun Young Yi
- Subjects
medicine.medical_specialty ,medicine.anatomical_structure ,business.industry ,Stomach ,medicine ,Sump Syndrome ,Drainage ,medicine.disease ,business ,Surgery ,Liver abscess - Published
- 2010
30. Thalidomide Induced Nonspecific Interstitial Pneumonia in Patient with Relapsed Multiple Myeloma
- Author
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Kyung-Nyeo Jeon, Hoon-Gu Kim, Ho Cheol Kim, Ji-Hyun Ju, Gyeong-Won Lee, Myung-Hee Kang, and Jung Hun Kang
- Subjects
medicine.medical_specialty ,Right middle lobe ,business.industry ,Radiography ,Case Report ,Middle Aged ,respiratory system ,Interstitial fibrosis ,medicine.disease ,Thalidomide ,respiratory tract diseases ,Surgery ,Lung diseases, interstitial ,Multiple myeloma ,Hemosiderin ,medicine ,Humans ,Female ,In patient ,Interstitial pneumonia ,Radiology ,business ,medicine.drug - Abstract
A 63-year-old female diagnosed with relapsed multiple myeloma visited our hospital complaining of a persistent cough. Since July 2006, she had been taking 100 mg thalidomide daily and gradually developed shortness of breath and a persistent dry cough. A chest X-ray and computed tomography showed ground glass opacities in both lungs. An open lung biopsy of the right middle lobe under general anesthesia revealed chronic peribronchial inflammation, mild interstitial fibrosis, and intra-alveolar macrophage infiltration, with some hemosiderin features, compatible with non-specific interstitial pneumonia (NSIP). After discontinuing the thalidomide, the patient's symptoms did not deteriorate, although the radiographs did not improve. The patient is alive and well with regular outpatient follow-up without progression of the NSIP.
- Published
- 2010
31. A Case of Fatal Hyperinfective Strongyloidiasis with Acute Respiratory Failure and Intestinal Perforation in Lung Cancer Patient
- Author
-
Eun Young Yun, Yu Eun Kim, Young Sil Hwang, Ho Cheol Kim, Gi Dong Lee, Hyeon Sik Kim, Jeong Eun Ma, Yu Ji Cho, Yi Yeong Jeong, Ji Hyun Ju, and Jong Deok Lee
- Subjects
medicine.medical_specialty ,Abdominal pain ,biology ,medicine.diagnostic_test ,business.industry ,fungi ,Perforation (oil well) ,medicine.disease ,biology.organism_classification ,Gastroenterology ,respiratory tract diseases ,Strongyloides stercoralis ,Surgery ,Strongyloidiasis ,medicine.anatomical_structure ,Bronchoscopy ,Internal medicine ,medicine ,Sputum ,Eosinophilia ,Esophagus ,medicine.symptom ,business - Abstract
Strongyloides stercoralis is an intestinal nematode that is a parasite to humans. The infecting filariform larvae of S. stercoralis enters the host body via the bloodstream, passes through the lungs, penetrates the alveoli, and then ascends the airway to transit down the esophagus into the small bowel. The infection can persist for decades without causing major symptoms and can elicit eosinophilia of varying magnitudes. Of note, this infection can also develop into a disseminated, often fatal, disease (hyperinfection) in patients receiving immunosuppressive corticosteroids. A 65-year-old man who was receiving corticosteroid therapy for the treatment of spinal stenosis was admitted to the emergency room with complaints of abdominal pain and severe dyspnea. We detected many S. stercoralis larvae in the sputum and in the bronchoalveolar-lavage sample collected by bronchoscopy. Here, we report a fatal case of strongyloidiasis with acute respiratory failure and intestinal perforation. In addition, we provide a brief review of the relevant medical literature.
- Published
- 2010
32. Successful Treatment of Syncope with Chemotherapy Irresponsive to Cardiac Pacemaker in Head and Neck Cancer
- Author
-
Myounghee Kang, Hoon Gu Kim, Ji Hyun Ju, Jin Pyeong Kim, Jung Je Park, Gyeong Won Lee, and Jung Hun Kang
- Subjects
Male ,Pacemaker, Artificial ,medicine.medical_specialty ,medicine.medical_treatment ,Tonsillar Neoplasms ,Case Report ,Docetaxel ,chemotherapy ,Malignancy ,Syncope ,Cardiac pacemaker ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,biology ,business.industry ,Wide local excision ,Head and neck cancer ,Carotid sinus ,Syncope (genus) ,Neck dissection ,General Medicine ,Middle Aged ,medicine.disease ,biology.organism_classification ,Surgery ,Radiography ,medicine.anatomical_structure ,Head and Neck Neoplasms ,Carcinoma, Squamous Cell ,head and neck cancer ,Taxoids ,Cisplatin ,Neoplasm Recurrence, Local ,business ,medicine.drug - Abstract
Recurrent syncope as a complication of recurrent neck malignancy is an uncommon but well documented association. The syncope is presumed to occur when a tumor mass invades the baroreceptor within the carotid sinus or when it disrupts the afferent nerve fibers of the glossopharyngeal nerve. A 59-year-old man presented with recurrent syncope and headache. He had a wide local excision including tonsillectomy and modified left radical neck dissection for tonsilar cancer 4 years ago. A computed tomography scan revealed ill-defined lesions in left parapharyngeal, carotid space and right upper jugular region. After clinical evaluation, cardiac pacemaker was placed, but he still suffered from the syncope. Then, he received the chemotherapy with docetaxel and cisplatin. The last hypotension event occurred on day 10 of the chemotherapy. Six months after 3 cycles of chemotherapy, he remained in complete remission and resolution of syncope. We report a case in which syncope was associated with a recurrence of tonsilar cancer and successfully treated with chemotherapy.
- Published
- 2009
33. An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses.
- Author
-
Yunho Gwak, Woongsic Jung, Yew Lee, Ji Sook Kim, Chul Geun Kim, Ji-Hyun Ju, Chihong Song, Jae-Kyung Hyun, and EonSeon Jin
- Subjects
ANTIFREEZE proteins ,MICROALGAE ,PROMOTERS (Genetics) ,DIATOMS ,CHLOROPLAST membranes ,IMMUNOGOLD labeling - Abstract
The structure and function of the Antarctic marine diatom Chaetoceros neogracile antifreeze protein (Cn-AFP), as well as its expression levels and characteristics of the ice-binding site, were analyzed in the present study. In silico analysis revealed that the Cn-AFP promoter contains both light- and temperature-responsive elements. Northern and Western blot analyses demonstrated that both Cn-AFP transcript and protein expression were strongly and rapidly stimulated by freezing, as well as temperature and high light stress. Immunogold labeling revealed that Cn-AFP is preferentially localized to the intracellular space near the chloroplast membrane. Recombinant Cn-AFP had clear antifreeze activity. Protein-folding simulation was used to predict the putative ice-binding sites in Cn-AFP, and site-directed mutagenesis of the Cn-AFP b-face confirmed their identification. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
34. A Case of Pure Red Cell Aplasia Associated with Hashimoto Disease
- Author
-
Jong Seok Lee, Kwang Min Kim, Gyeong Won Lee, Hoon Gu Kim, Myung Hee Kang, Ji Hyun Ju, and Jung Hun Kang
- Subjects
Autoimmune disease ,medicine.diagnostic_test ,business.industry ,Anemia ,Pure red cell aplasia ,Hematology ,Hematocrit ,urologic and male genital diseases ,medicine.disease ,Mixed connective tissue disease ,Rheumatoid arthritis ,Immunology ,medicine ,Hashimoto Disease ,Reticulocytopenia ,business - Abstract
Pure red cell aplasia (PRCA) is a rare hematologic disorder characterized by anemia, reticulocytopenia in the blood and isolated severe erythroblastopenia with no abnormalities of the granulopoiesis and mega- karyopoiesis in the bone marrow. Acquired PRCA has been reported in association with various auto- immune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis, mixed connective tissue disease and Sjogren's syndrome. We report here on a case of PRCA associated with Hashimoto disease (without any other autoimmune disease) which, to the best of our knowledge, has not been pre- viously reported in Korean and English literatures. Our patient was treated with levothyroxine alone with- out other immunosuppressive agents and her hemoglobin concentration and hematocrit values returned
- Published
- 2007
35. Four Cycles of Docetaxel and Cisplatin Combination Chemotherapy in Patients with Advanced Non-small Cell Lung Cancer
- Author
-
Ji-Hyun Ju, Seung Suk Yoou, Gyeong-won Lee, Hoon Gu Kim, Sang-min Lee, Won Sup Lee, Myung Hee Kang, Jung Hun Kang, and Kwang Min Kim
- Subjects
Oncology ,Chemotherapy ,medicine.medical_specialty ,Performance status ,business.industry ,medicine.medical_treatment ,Combination chemotherapy ,Neutropenia ,medicine.disease ,Vinorelbine ,Docetaxel ,Internal medicine ,medicine ,Lung cancer ,business ,Febrile neutropenia ,medicine.drug - Abstract
Purpose: We performed a pilot study on defining duration of docetaxel and cisplatin combination chemotherapy in patients with advanced non-small cell lung cancer to evaluate its efficacy. Patients and Methods: Sixteen chemonaive patients with biopsy proven, unresectable (stage IIIB or IV) non-small cell lung cancer (NSCLC) were enrolled between January 2003 and December 2004. Treatment consisted of docetaxel (75 mg/m/day) and cisplatin (70 mg/m/day) every 3 week up to 4 cycles. The outcome was compared with that of a historical control group of 42 patients treated from January 1998 until December 2001, who were treated with mitomycin-C, vinorelbine, cisplatin for unresectable stage IIIB or IV NSCLC. Results: Median age was 68 (age range 43∼72). Among 16 patients, 5 patients were stage IIIB and 11 were stage IV. Fourteen patients had performance status of 0-1 and, 2 had performace status 2 respectively. Two patients were lost due to refusal of receiving chemotherapy. By intention-totreat-analysis, overall response rate was 44% (C.I.: 0.19∼0.68). No complete response was noted. Median time to progression (TTP) was 144 days. Overall survival (OS) was 285 days. There is no difference in TTP & OS between docetaxel, cispatin (DP) group and mitomycin, vinorelbine, cisplatin (MVP) group statistically. Neutropenia was the most common grade III or IV toxicity; (two patients had Grade III toxicity and five Grade IV toxicity). Five patients developed febrile neutropenia, and three of neutropenia patients died due to pneumonia or septic shock. The most common non-hematologic toxicity was infection. Two of 3 patients with infection required admission. Conclusion: These findings suggested that four cycles of docetaxel and cisplatin might be as effective as MVP continous therapy for advanced NSCLC. (J Lung Cancer 2007;6(1):8 14)
- Published
- 2007
36. Clinical Implications of Topographic Anatomy on the Ganglion Impar
- Author
-
Oh, Chang-Seok, primary, Chung, In-Hyuk, additional, Ji, Hyun-Ju, additional, and Yoon, Duck-Mi, additional
- Published
- 2004
- Full Text
- View/download PDF
37. The Muscular Branch of the Radial Nerve to the Brachialis Muscle in Korean
- Author
-
Ji, Hyun Ju, primary and Chung, In Hyuk, additional
- Published
- 2002
- Full Text
- View/download PDF
38. HER2 stabilizes survivin while concomitantly down-regulating survivin gene transcription by suppressing Notch cleavage.
- Author
-
Ji-hyun JU, Wonseok YANG, Sunhwa OH, KeeSoo NAM, Kyung-min LEE, Dong-young NOH, and Incheol SHIN
- Subjects
- *
BREAST cancer , *GROWTH factors , *CELL communication , *GENETIC regulation , *SURVIVIN (Protein) , *PHOSPHORYLATION - Abstract
In breast cancer, the HER2 (human epidermal growth factor receptor 2) receptor tyrosine kinase is associated with extremely poor prognosis and survival. Notch signalling has a key role in cell-fate decisions, especially in cancer-initiating cells. The Notch intracellular domain produced by Notch cleavage is translocated to the nucleus where it activates transcription of target genes. To determine the combinatory effect of HER2 and Notch signalling in breast cancer, we investigated the effect of HER2 on Notch-induced cellular phenomena. We found the down-regulation of Notch-dependent transcriptional activity by HER2 overexpression. Also, the HER2/ERK (extracellularsignal- regulated kinase) signal pathway down-regulated the activity of γ-secretase. When we examined the protein level of Notch target genes in HER2-overexpressing cells, we observed that the level of survivin, downstream of Notch, increased in HER2 cells. We found that activation of ERK resulted in a decrease in XAF1 [XIAP (X-linked inhibitor of apoptosis)- associated factor 1] which reduced the formation of the XIAP-XAF1 E3 ligase complex to ubiquitinate survivin. In addition, Thr34 of survivin was shown to be the most important residue in determining survivin stability upon phosphorylation after HER2/Akt/CDK1 (cyclin-dependent kinase 1)-cyclin B1 signalling. The results of the present study showthe combinatorial effects of HER2 and Notch during breast oncogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
39. Protein expression profiling of primary mammary epithelial cells derived from MMTV-neu mice revealed that HER2/NEU-driven changes in protein expression are functionally clustered.
- Author
-
Sungwoo Park, Kyung-min Lee, Ji-hyun Ju, Jaeyoon Kim, Dong-Young Noh, Taehoon Lee, and Shin, Incheol
- Subjects
EPITHELIAL cells ,LABORATORY mice ,PROTEINS ,APOPTOSIS ,ANIMAL models in research - Abstract
MMTV-neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV-neu heterozygote mice and wild type (WT) littermates using two-dimensional digest (ChemDigest™/Trypsin)-LC-MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti-apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion-related proteins were downregulated in PMECs from MMTV-neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU-driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010 [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
40. Successful Treatment of Syncope with Chemotherapy Irresponsive to Cardiac Pacemaker in Head and Neck Cancer.
- Author
-
Ji Hyun Ju, Myoung Hee Kang, Hoon Gu Kim, Gyeong Won Lee, Park, Jung Je, Jin Pyeong Kim, and Jung Hun Kang
- Abstract
Recurrent syncope as a complication of recurrent neck malignancy is an uncommon but well documented association. The syncope is presumed to occur when a tumor mass invades the baroreceptor within the carotid sinus or when it disrupts the afferent nerve fibers of the glossopharyngeal nerve. A 59-year-old man presented with recurrent syncope and headache. He had a wide local excision including tonsillectomy and modified left radical neck dissection for tonsilar cancer 4 years ago. A computed tomography scan revealed ill-defined lesions in left parapharyngeal, carotid space and right upper jugular region. After clinical evaluation, cardiac pacemaker was placed, but he still suffered from the syncope. Then, he received the chemotherapy with docetaxel and cisplatin. The last hypotension event occurred on day 10 of the chemotherapy. Six months after 3 cycles of chemotherapy, he remained in complete remission and resolution of syncope. We report a case in which syncope was associated with a recurrence of tonsilar cancer and successfully treated with chemotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
41. Phase II trial of S-1 in combination with gemcitabine for chemo-naïve patients with locally advanced or metastatic pancreatic cancer.
- Author
-
Gyeong-Won Lee, Hye Junh Kim, Ji-Hyun Ju, Seok-Hyun Kim, Hoon Gu Kim, Tae Hyo Kim, Hyun Jin Kim, Chi-Young Jeong, and Jung Hun Kang
- Subjects
RESEARCH ,PANCREATIC cancer ,COMBINATION drug therapy ,CANCER treatment ,THERAPEUTICS ,DRUGS - Abstract
We performed a phase II study of combination chemotherapy with S-1 plus gemcitabine for treating chemo-naïve patients with unresectable pancreatic cancer to evaluate the efficacy and toxicity. Patients with histologically confirmed unresectable pancreatic cancer were eligible. The treatment consisted of S-1 (40 mg/m
2 p.o. b.i.d. from D1 to 14) and gemcitabine (1,250 mg/m2 on D1 and 8), repeated every 3 weeks. Thirty-two patients were enrolled between March 2005 and December 2007. No complete response was observed and a partial response was observed in 14 patients (44.0%), stable disease in eight patients (25.0%), and progressive disease in eight patients (25.0%). The median time to progression was 4.92 months (95% CI: 4.16–5.67 months), and the median overall survival was 7.89 months (95% CI: 5.96–9.82 months). The survival duration was significantly longer for the patients with a good performance status compared with that of the patients with a poor performance status. The major toxicities were grade 3–4 neutropenia (9, 28.1%), grade 3/4 thrombocytopenia (5, 15.6%), and grade 3 diarrhea (5, 15.6%). The combination chemotherapy of S-1 and gemcitabine showed promising antitumor activity and manageable toxicities, and especially for the good performance status patients with unresectable pancreatic cancer. [ABSTRACT FROM AUTHOR]- Published
- 2009
- Full Text
- View/download PDF
42. Repeated Therapeutic Embolization with Systemic Chemotherapy for Hepatic Metastases from !- Fetoprotein-producing Gastric Cancer .
- Author
-
Ji-Hyun Ju, Won Sup Lee, Myung Hee Kang, Seok Hyun Kim, Hoon Gu Kim, Dong Chul Kim, Tae-Beom Shin, and Kyungsoo Bae
- Subjects
- *
CANCER chemotherapy , *THERAPEUTIC embolization , *STOMACH cancer , *LIVER cancer , *COMBINATION drug therapy , *HEPATIC veno-occlusive disease - Abstract
α-Fetoprotein (AFP)-producing gastric cancer is rare, characterized by high malignant potential (high proliferative activity, lack of apoptosis, and rich neovascularization), and associated with a dismal prognosis when it metastasizes to the liver. We present here a case of metastatic α-fetoprotein-producing gastric cancer to the liver in a 72-year-old man who was treated with repeated hepatic transarterial embolization (TAE) in combination with systemic chemotherapy. The tumor metastasis controlling effects seen may suggest a therapeutic option in these cases. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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