1. A small heat shock protein 21 (sHSP21) mediates immune responses in Chinese oak silkworm Antheraea pernyi
- Author
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Bo-Ping Tang, Li Chaofeng, Ji-Wu Tian, Yang Li, Li Chen, Xun-Guang Bian, Yu Liu, Dong Wang, Chun-Lin Zhou, Xiao-Yu Zhu, Bao-Ming Ge, Qiu-Ning Liu, and Zhao-Zhe Xin
- Subjects
Lipopolysaccharides ,0301 basic medicine ,Antheraea pernyi ,Moths ,Biology ,Biochemistry ,Quercus ,03 medical and health sciences ,Protein Domains ,Structural Biology ,Heat shock protein ,Animals ,Cloning, Molecular ,Binding site ,Molecular Biology ,Gene ,Phylogeny ,Binding Sites ,Innate immune system ,Kinase ,General Medicine ,biology.organism_classification ,Nucleopolyhedroviruses ,Heat-Shock Proteins, Small ,Cell biology ,Immunity, Active ,030104 developmental biology ,Gene Expression Regulation ,Insect Proteins ,Casein kinase 2 ,Tyrosine kinase - Abstract
Small heat shock proteins (sHSPs) are conserved among insects and play an important role in the regulation of many biological processes, including temperature stress, abiotic stress, immune responses, metamorphosis, and embryo development. Antheraea pernyi is an economically valuable silk-producing moth and source of insect food containing high-quality protein. The aim of this study was to quantify expression of the ApsHSP21 gene in response to pathogen-associated molecular patterns (PAMPs) and nucleopolyhedrovirus (NPV) challenge. The deduced ApsHSP21 protein sequence consists of 186 residues with a calculated molecular mass of 21.0 kDa and an isoelectronic point (pI) of 6.63. The protein contains a conserved α-crystallin domain (ACD), and includes two casein kinase II phosphorylation sites, a protein kinase C phosphorylation site, two tyrosine kinase phosphorylation sites, and various polypeptide binding sites. Phylogenetic analysis revealed that ApsHSP21 is closely related to homologs from other insects. Real-time quantitative reverse transcription PCR (qRT-PCR) analysis revealed that expression of ApsHSP21 was significantly up-regulated at different timepoints following simulated pathogen challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), glucan, and NPV. The results suggest sHSP21 is involved in innate immune responses in A. pernyi.
- Published
- 2018