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11 results on '"Jia, Bao Xiu"'

2. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling

Author

Chen Jiao, Yang Zhen, Guo Yu-rong, Jia Bao-xiu, Liu Cai-hong, Li Yuqin, and You Guirong

Subjects
Models, Molecular, Circular dichroism, Time Factors, Article Subject, Serum albumin, lcsh:Medicine, Microscopy, Atomic Force, Protein Structure, Secondary, General Biochemistry, Genetics and Molecular Biology, Hydrophobic effect, Patulin, chemistry.chemical_compound, medicine, Humans, Molecule, Serum Albumin, Binding Sites, Quenching (fluorescence), General Immunology and Microbiology, biology, Hydrogen bond, Circular Dichroism, lcsh:R, General Medicine, Human serum albumin, body regions, Kinetics, Spectrometry, Fluorescence, Energy Transfer, chemistry, biology.protein, Thermodynamics, Physical chemistry, Spectrophotometry, Ultraviolet, Research Article, Protein Binding, medicine.drug
Abstract

The interaction of patulin with human serum albumin (HSA) was studied in vitro under normal physiological conditions. The study was performed using fluorescence, ultraviolet-visible spectroscopy (UV-Vis), circular dichroism (CD), atomic force microscopy (AFM), and molecular modeling techniques. The quenching mechanism was investigated using the association constants, the number of binding sites, and basic thermodynamic parameters. A dynamic quenching mechanism occurred between HSA and patulin, and the binding constants (K) were 2.60 × 104, 4.59 × 104, and 7.01 × 104 M−1at 288, 300, and 310 K, respectively. Based on fluorescence resonance energy transfer, the distance between the HSA and patulin was determined to be 2.847 nm. TheΔG0,ΔH0, andΔS0values across various temperatures indicated that hydrophobic interaction was the predominant binding force. The UV-Vis and CD results confirmed that the secondary structure of HSA was altered in the presence of patulin. The AFM results revealed that the individual HSA molecule dimensions were larger after interaction with patulin. In addition, molecular modeling showed that the patulin-HSA complex was stabilized by hydrophobic and hydrogen bond forces. The study results suggested that a weak intermolecular interaction occurred between patulin and HSA. Overall, the results are potentially useful for elucidating the toxigenicity of patulin when it is combined with the biomolecular function effect, transmembrane transport, toxicological, testing and other experiments.

Published
2014
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8. Flow injection determination of metoclopramide based on KMnO4—HCHO chemiluminescence in a micellar medium

Author

Jia, Bao-xiu, Li, Yu-qin, Liu, Cai-hong, Li, Ke, and Qi, Yong-xiu

Subjects
*FLOW injection analysis, *AMIDES, *POTASSIUM permanganate, *CHEMILUMINESCENCE, *MICELLES, *LUMINESCENCE, *SURFACE active agents
Abstract

Abstract: A novel flow injection chemiluminescence (CL) analysis method for the determination of metoclopramide in the presence of sodium dodecyl sulfonate (SDS) surfactant micelles is described. This method is based on the luminescent properties of the KMnO4–HCHO–metoclopramide in acid medium sensitized by SDS. The optimized experimental conditions were evaluated and the possible mechanism was discussed. The CL increase is linearly related to the concentration of metoclopramide in the range 0–80.0μg/ml with a detection limit of 31.3ng/mL (S/N=3).The relative standard deviation for 20.0μg/ml samples was 2.6% (n=11). The proposed method has been successfully applied to the determination of metoclopramide in tablets. [ABSTRACT FROM AUTHOR]

Published
2010
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11. [Determination of tetrandrine and fangchinoline in Radix Stephaniae tetrandrae and its preparation by nonaqueous capillary chromatography].

Author

Li YQ, Chen XG, Qi YX, Li K, Jia BX, Liu CH, and Cao ML

Subjects
Calibration, Capsules, Drugs, Chinese Herbal isolation & purification, Drugs, Chinese Herbal standards, Plant Roots chemistry, Plants, Medicinal chemistry, Quality Control, Reproducibility of Results, Benzylisoquinolines analysis, Capillary Electrochromatography methods, Drugs, Chinese Herbal chemistry, Stephania tetrandra chemistry
Abstract

Objective: To establish a new method for the determination of fangchinoline and tetrandrine in Stephania tetrandra and Fengtongan capsule by noanqueous capillary electrophoresis., Method: Separation was carried out in an uncoated fused capillary (50 cm x 75 microm i.d.) with a running buffer containing 50 mmol x L(-1) ammonium acetate, 1.0% acetic acid and 20% acetonitrile in methanol. A separation voltage of 20 kV and a UV detector wavelength at 214 nm were adopted. Sample was introduced from the anode., Result: The calibration ranges were 1.00, 500 mg x L(-1) for both analytes. Under the optimum conditions, the relative standard deviation (RSD, n = 6) for the migration time of each analyte were 0.09%, 1.9% (intra-day) and 0.63%, 1.9% (inter-day); The RSD for the peak area of each analyte were 0.45%, 5.9% (intra-day) and 2.3%, 5.6% (inter-day), respectively. The contents of the analytes were determined easily with average recoveries 102% for fangchinoline and 105% for tetrandrine in S. tetrandra and 94.6% for fangchinoline and 98.7% for tetrandrine in Fengtongan capsules, respectively., Conclusion: The proposed method is simple, rapid, accurate and higher repeatable, and can be used to control of the quality of S. tetrandra and Fengtongan capsules.

Published
2007

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