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• A sandwich assay for β-lactoglobulin (β-LG) in infant food formula was developed. • Hierarchical antifouling capture probe and fluorescent recognition probe were used. • Capture probe selectively recognized β-LG and resisted nonspecific protein adhesion. • Recognition probe exhibited fluorescent signal upon interaction with β-LG by aptamer. • Promising detection performance in commercial food samples was achieved. Tracing the presence of allergenic β-lactoglobulin (β-Lg) in infant foods is an urgent need, but the interference from the protein-rich matrix often hampered the detection accuracy. Here, we developed a sandwich assay for β-Lg in infant food formula based on a hierarchically architectured antifouling capture probe and fluorescent recognition probe. The antifouling capture probe was constructed from the polydopamine-coated magnetic particles (Fe 3 O 4 @PDA), which was modified with repeated glutamic acid-lysine (EK) antifouling peptide and aptamer towards β-Lg. The spatial arrangement of these ligands on the Fe 3 O 4 @PDA surface was carefully tailored. Furthermore, a fluorescent recognition probe based on aptamer-modified silica-doped carbon quantum dot was developed to explore a sandwich assay for β-Lg with the capture probe. The sandwich assay was proved to have high potential in detecting β-Lg in commercially available infant food samples. The work provided a new approach to developing detection methods with matrix interference-resistant properties. [ABSTRACT FROM AUTHOR]

The coseismic geothermal changes of ground temperature observed at observatories near the epicenter of the 2020 Jiashi Ms = 6.4 earthquake in China, provide a unique opportunity to study heat generation and conduction in rock. Here, evolutions of rock temperature at the Xikeer, Jiashizongchang, and Gedaliang observatories, which are located at epicentral distances of 1.4, 27.42, and 50 km respectively, were analyzed. Significant coseismic geothermal changes of 0.0432 °C were observed at the Xikeer observatory at the depth of 33.38 m, at which clear diurnal variations can be observed. Smaller changes of ~0.0001 °C were observed at the depths of 12.3 and 22.8 m at the Xikeer observatory and 22.3 m at the Jiashizongchang observatory. The stress transfer induced by the coseismic rupture induced a rise in local ground temperature, but the magnitude of the change was relatively small. The larger amplitude change at the Xikeer observatory was caused by fluid infiltration. We note that diurnal variation has been recorded at the Gedaliang observatory, but the coseismic response is no longer in existence. The temperature increases at the hypocentral area were higher than expected in the ground due to the coseismic stress transfer, but the change attenuated rapidly with distance. [ABSTRACT FROM AUTHOR]

This document is a retraction notice from the journal OncoTargets & Therapy regarding an article titled "MLPH Accelerates the Epithelial–Mesenchymal Transition in Prostate Cancer." The retraction is due to concerns about the duplication of images from Figure 3 with images from other unrelated articles. The authors did not respond to queries or provide an explanation for the duplicated images. The retracted article will remain online but will be digitally watermarked as "Retracted." [Extracted from the article]

The solar radiation or insolation at a specific locality on the Earth's surface is determined by the distance of this point from the sun and by the angle with which the radiation hits the earth's surface. The theory that variations in the earth's orbit determine the amount of radiation and therefore the climate which is the Milankovitch Cycle. In non‐marine lacustrine rift basin, the changes of climate can be recorded by the palaeontology, and it also plays an important role in controlling lacustrine fluctuations. The lacustrine fluctuations are indicated by coastal onlap, which is the landward or lake‐ward onlap of non‐marine coastal deposits. In this paper, based on borehole data, wireline logs, seismic datasets, biostratigraphic data and palaeontology, the lacustrine fluctuation curve can be reconstructed from the onlap of lacustrine deposits in non‐marine sequences. The durations and magnitudes of these changes can be used to construct charts showing cycles of the relative rising and falling of lake level. In addition, the changes of lake level can also be reflected by the palaeontology such as pollen. The living conditions and distributions of pollen can be a good indicator for climate changes and lacustrine fluctuations in an a non‐marine setting. The calculation of the Milankovitch Cycle can be determined by the GR log in the study area which keeps a record of cycle changes. All climatic changes and lacustrine fluctuations are mainly resulted from the Milankovitch Cycles, and therefore, through the reconstruction of lacustrine fluctuations, climate changes and the Milankovitch Cycle, the correspondences among them can be clearly addressed. All the results can contribute a lot in theory research in the study area and lead to a new focus in practical hydrocarbon exploration and exploitation. [ABSTRACT FROM AUTHOR]

Introduction: Prostate cancer (PC) is the second greatest cause of cancer deaths globally. PC presents a poor prognosis once it metastasizes. There is considerable proof of vital epithelial–mesenchymal transition (EMT) functionality in PC metastasis. Previous studies revealed that melanophilin (MLPH) is associated with PC; however, its role in PC remains poorly understood. Methods: Bioinformatics analyses were performed. The cellular responses to MLPH knockdown were examined in HCC cell lines via wound healing assay, migration and invasion assay, Western blotting. Results: Analysis of the PROGgeneV2 database revealed that high MLPH expression might indicate poor overall survival. MLPH knockdown reduced PC cell migration, proliferation, and invasion. MLPH downregulation in vivo resulted in a lower growth rate and fewer metastatic nodules in lung tissues. Furthermore, MLPH knockdown recovered downregulated expression of the mesenchymal marker N-cadherin and the epithelial marker E-cadherin following a decrease in β-catenin. Conclusion: These results indicate that progression of PC is stimulated via MLPH-dependent initiation of the EMT. [ABSTRACT FROM AUTHOR]

Stress may induce apparent resistivity changes. Clarifying the deformation process of the source media is critical for determining the correlations between resistivity variations and earthquake occurrence. In this study, the stress state of a medium was analyzed by integrating GPS measurements, the spatiotemporal evolution of the load/unload response ratio (LURR), geochemical monitoring, and synchronous apparent resistivity changes preceding the 2020 Mw 6.0 Jiashi earthquake. The medium hosting the Kalpin Observatory underwent elastic deformation before 2019, and the synchronous decreases in the E–W and N–S apparent resistivities from 2015 can be attributed to N–S-dominated compressive stress. The microdamage stage occurred in 2019, with subsequent E–W apparent resistivity variation amplitudes that were ~0.4 Ωm higher than those in previous years. This difference is a result of microdamage to the medium owing to tensile stress during the seismogenic process. The spatiotemporal evolution of the LURR and gas seepage monitoring data also indicate that the medium was damaged prior to the earthquake. Variations in the apparent resistivity measured at the Kalpin Observatory indicate that the medium underwent elastic deformation, followed by microdamage, until stress triggered the earthquake. [ABSTRACT FROM AUTHOR]

Adipose derived stem cells (ADSCs) have the property to differentiate into neuron-like cells, which may provide a novel insight for the restoration of erectile dysfunction (ED) mainly induced by cavernous nerve injury. Icariside II (ICA II) has been reported to play a key role in the regulation of erectile function via stimulating the differentiation of ADSCs to Schwann Cells (SCs). However, the function and molecular mechanisms of ICA II in ED remains to be further clarified. The expression of S100, P75, GDNF and miR-33 was detected by qRT-PCR. And the relative proteins expression was determined by western blot. Cell viability was measured by Cell Counting Kit-8 (CCK-8) assay. Bioinformatics, luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify the interaction between miR-33 and GDNF. Intracavernosal pressure (ICP), the ratio of ICP and mean arterial pressure (MAP), as well as nNOS expression were examined to evaluate the erectile function of SD rats with bilateral cavernous nerve injury (BCNI). ICA II and miR-33 respectively promoted and inhibited the differentiation of ADSCs to SCs. MiR-33 could negatively regulate P75 and GDNF expression. ICA II exerted promotion effects on differentiation of ADSCs to SCs via regulating miR-33. GDNF was identified to be a target of miR-33. MiR-33 overexpression abrogated the stimulatory effect of ICA II on ADSCs' differentiation, which was blocked by GDNF overexpression. treated with ICA II recovered the erectile function of BCNI model rats through regulation of miR-33. ICA II contributed to the differentiation of ADSCs to SCs viamiR-33/GDNF axis, contributing to the recovery of erectile function in BCNI rats. [ABSTRACT FROM AUTHOR]