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21 results on '"Jia, Guangle"'

4. High-frequency inlaid cylindrical piezoelectric transducer for high-sensitivity acoustics.

Author

Rong, Tian, Luo, Qinghua, Wang, Chenxu, Li, Yingchun, Jia, GuangLe, and Yu, Zhaoxian

Subjects
PIEZOELECTRIC transducers, ACOUSTICS, TRANSDUCERS, FINITE element method, CIRCUIT elements
Abstract

This work develops a high-frequency high-sensitivity inlaid cylindrical hydroacoustic transducer by enhancing the electromechanical coupling coefficient, amplifying stress, and adopting circumferential inlaid techniques. Equivalent circuit and finite element simulation methods were used to determine the optimum parameters of the unit, then the transducer was fabricated. Tests show that the resonant frequency of every array unit was 220 kHz. The maximum emission voltage response and reception sensitivity were respectively about 172 dB and −177 dB, with emission and reception bandwidths both about 40 kHz at 3 dB loss. The horizontal and vertical beamwidths were 15° and 4° for each unit respectively. [ABSTRACT FROM AUTHOR]

Published
2023
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13. Levenberg-Marquardt Optimization Based Iterative Divided Difference Filtering for Multi-AUV Cooperative Localization

Author

Jia Guangle, Gao Wei, Zhang Yong-gang, Sun Chengjiao, and Luo Li

Subjects
020301 aerospace & aeronautics, 0209 industrial biotechnology, Computer science, Initialization, 02 engineering and technology, Filter (signal processing), Levenberg–Marquardt algorithm, 020901 industrial engineering & automation, 0203 mechanical engineering, Transmission (telecommunications), State (computer science), Underwater acoustics, Algorithm, Underwater acoustic communication
Abstract

Due to the low communication frequency and slow transmission speed of underwater acoustic communication, the frequency of cooperative update is significantly reduced, which results in poor filtering convergence and positioning accuracy. To solve this problem, in this paper, a new iterative divided difference filter (IDDF) is proposed for multi-AUV cooperative localization system based on a Levenberg-Marquardt optimization. As compared with conventional filtering algorithm, the proposed IDDF algorithm can improve the convergence and estimation accuracy when the state estimation has large initialization error. Simulation results show that the proposed cooperative localization algorithm has better filtering convergence and positioning accuracy then existing methods.

Published
2018

17. [Oligonucleotide microarray for subtyping avian influenza virus]

Author

Han, Xueqing, Lin, Xiangmei, Hou, Yihong, Wu, Shaoqiang, Liu, Jian, Mei, Lin, Jia, Guangle, and Yang, Zexiao

Subjects
Birds, Viral Proteins, Influenza A virus, Reverse Transcriptase Polymerase Chain Reaction, Influenza in Birds, Influenza, Human, Animals, Humans, Neuraminidase, Hemagglutinin Glycoproteins, Influenza Virus, DNA Primers, Oligonucleotide Array Sequence Analysis
Abstract

Avian influenza viruses are important human and animal respiratory pathogens and rapid diagnosis of novel emerging avian influenza viruses is vital for effective global influenza surveillance. We developed an oligonucleotide microarray-based method for subtyping all avian influenza virus (16 HA and 9 NA subtypes).In total 25 pairs of primers specific for different subtypes and 1 pair of universal primers were carefully designed based on the genomic sequences of influenza A viruses retrieved from GenBank database. Several multiplex RT-PCR methods were then developed, and the target cDNAs of 25 subtype viruses were amplified by RT-PCR or overlapping PCR for evaluating the microarray. Further 52 oligonucleotide probes specific for all 25 subtype viruses were designed according to published gene sequences of avian influenza viruses in amplified target cDNAs domains, and a microarray for subtyping influenza A virus was developed. Then its specificity and sensitivity were validated by using different subtype strains and 2653 samples from 49 different areas.The results showed that all the subtypes of influenza virus could be identified simultaneously on this microarray with high sensitivity, which could reach to 2.47 pfu/mL virus or 2.5 ng target DNA. Furthermore, there was no cross reaction with other avian respiratory virus.An oligonucleotide microarray-based strategy for detection of avian influenza viruses has been developed. Such a diagnostic microarray will be useful in discovering and identifying all subtypes of avian influenza virus.

Published
2008

21. Development of a DNA barcoding system for the Ixodida (Acari: Ixodida).

Author

Lv, Jizhou, Wu, Shaoqiang, Zhang, Yongning, Zhang, Tianyi, Feng, Chunyan, Jia, Guangle, and Lin, Xiangmei

Subjects
ACAROLOGY, MITES, DEOXYRIBOSE, ARTHROPODA, TICK-borne diseases
Abstract

To control the spread of tick-borne diseases, there is an urgent need to develop a reliable technique that can distinguish different species of ticks. DNA barcoding has been proved to be a powerful tool to identify species of arthropods, but this technique has not yet been developed for identifying ticks. Here, we screened and analyzed 1082 sequences of ticks from BOLD system and GenBank, consisting of 647 16S, 325 COI, and 110 18S. These sequences are reported in previous studies and considered to be correctly identified at the species level. Through the analyses of genetic divergences and neighbor-joining (NJ) phylogenetic relationships between the species of ticks, our results show that COI and 16S are reliable in discriminating species of ticks and the 18S could discriminate ticks at the genera level. New universal primers for 16S, 18S, and COI of ticks were designed and a DNA barcoding system for the Ixodida was developed. To assess the performance of this system, 57 specimens of ticks were collected within China. Our results show that DNA barcoding system could correctly identify the species of specimens in adult and subadult stages. This system would assist non-taxonomists to conveniently identify the species of Ixodida based on DNA sequences rather than morphological traits. However, there are still serious deficiencies in the information of 16S and COI of some species of ticks, and additional research is needed to resolve this problem. [ABSTRACT FROM AUTHOR]

Published
2014
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