1. Heterologous boost with mRNA vaccines against SARS-CoV-2 Delta/Omicron variants following an inactivated whole-virus vaccine.
- Author
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Lu, Changrui, Zhang, Yuntao, Liu, Xiaohu, Hou, Fujun, Cai, Rujie, Yu, Zhibin, Liu, Fei, Yang, Guohuan, Ding, Jun, Xu, Jiang, Hua, Xianwu, Cheng, Xinhua, Pan, Xinping, Liu, Lianxiao, Lin, Kang, Wang, Zejun, Li, Xinguo, Lu, Jia, Zhang, Qiu, Li, Yuwei, Hu, Chunxia, Fan, Huifen, Liu, Xiaoke, Wang, Hui, Jia, Rui, Xu, Fangjingwei, Wang, Xuewei, Huang, Hongwei, Zhao, Ronghua, Li, Jing, Cheng, Hang, Jia, William, and Yang, Xiaoming
- Subjects
Epidemic ,Virus ,mRNA vaccine ,Animals ,Humans ,COVID-19 ,COVID-19 Vaccines ,SARS-CoV-2 ,Antibodies ,Neutralizing ,mRNA Vaccines ,Antibodies ,Viral ,Vaccines ,Inactivated - Abstract
The coronavirus SARS-CoV-2 has mutated quickly and caused significant global damage. This study characterizes two mRNA vaccines ZSVG-02 (Delta) and ZSVG-02-O (Omicron BA.1), and associating heterologous prime-boost strategy following the prime of a most widely administrated inactivated whole-virus vaccine (BBIBP-CorV). The ZSVG-02-O induces neutralizing antibodies that effectively cross-react with Omicron subvariants. In naïve animals, ZSVG-02 or ZSVG-02-O induce humoral responses skewed to the vaccines targeting strains, but cellular immune responses cross-react to all variants of concern (VOCs) tested. Following heterologous prime-boost regimes, animals present comparable neutralizing antibody levels and superior protection against Delta and Omicron BA.1variants. Single-boost only generated ancestral and omicron dual-responsive antibodies, probably by recall and reshape the prime immunity. New Omicron-specific antibody populations, however, appeared only following the second boost with ZSVG-02-O. Overall, our results support a heterologous boost with ZSVG-02-O, providing the best protection against current VOCs in inactivated virus vaccine-primed populations.
- Published
- 2023