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With the development of the photovoltaic industry; there will be an increasing demand for efficient, high-power density, and low-cost grid interface converters. Compared with two-level inverters, multilevel inverters have the following advantages: (1) lower device voltage ratings; (2) better output filtering spectrum; (3) lower electromagnetic interference (EMI) noise; and (4) higher switching speed capability. However, the complex switching circuit of the multilevel inverter will bring more parasitic inductance, resulting in severe switching overvoltage (ringing). Especially in order to reduce the cost of the inverter, using the long-loop modulation mode, the commutation loop will introduce more parasitic inductance, which will make the overvoltage more serious. Consider that commonly used overvoltage absorption schemes are effective only for overvoltage or suppression of oscillations. Therefore, a new overvoltage absorption circuit is proposed in this paper, which can not only alleviate the overvoltage and ringing phenomena but also suppress the effect of voltage jumps during low-frequency switching on high-frequency input voltage. This overvoltage absorption circuit is characterized by low overvoltage, fast ringing damping, and minimum capacitance. Experiments and simulations are conducted to verify the effectiveness of this overvoltage absorption circuit using a three-level ANPC inverter as a prototype. The results show that the proposed overvoltage absorption circuit can significantly reduce the overvoltage level, shorten the oscillation time, and reduce the voltage difference between the upper and lower DC bus capacitors. [ABSTRACT FROM AUTHOR]

Bacillus cereus belongs to Gram-positive bacteria, which is widely distributed in nature and shows certain pathogenicity. Different B . cereus strains carry different subsets of virulence factors, which directly determine the difference in their pathogenicity. It is therefore important to study the distribution of virulence factors and the biological activity of specific toxins for precise prevention and control of B . cereus infection. In this study, the hemolysin BL triayl was expressed, purified, and characterized. The results showed that the bovine pathogenic B . cereus hemolysin BL could be expressed and purified in the prokaryotic expression system, and the bovine pathogenic B . cereus hemolysin BL showed hemolysis, cytotoxicity, good immunogenicity and certain immune protection in mice. In this study, the recombinant expression of hemolysin BL triayl was achieved, and the biological activity of hemolysin BL of bovine pathogenic ceroid spore was investigated. This study may facilitate further investigating the pathogenic mechanism of B . cereus hemolysin BL and developing a detection method for bovine pathogenic B . cereus disease.