28 results on '"Jia-zhou Li"'
Search Results
2. Comparative study on pyrolysis kinetics of agroforestry biomass based on distributed activation energy model method
- Author
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Pei QIAO, Zi-qian GUO, Yu-ming ZHANG, Jia-zhou LI, Wei ZHANG, and Ming-hua LIU
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- 2022
3. Comparative pyrolysis kinetics of heavy oil and its SARA fractions using distributed activation energy model
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Qing-An Xiong, Yu-Ming Zhang, Pei Qiao, Guo-Tong Li, Jia-Zhou Li, and Wei Zhang
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Geophysics ,Fuel Technology ,Geochemistry and Petrology ,Energy Engineering and Power Technology ,Economic Geology ,Geology ,Geotechnical Engineering and Engineering Geology - Published
- 2023
4. Transformation behaviors of vanadium and nickel during petroleum coke and coal co-gasification
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Jia zhou Li and Bing Wang
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Fuel Technology ,General Chemical Engineering ,Energy Engineering and Power Technology ,General Chemistry ,Geotechnical Engineering and Engineering Geology - Published
- 2022
5. Design and Implementation of an Augmented Reality-based Interactive and Real-time Wind Turbine Maintenance Auxiliary Platform System.
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Jui-Hung Liu, Chen-Yi Tsai, Jia-Zhou Li, and Chun-Chieh Wang
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AUGMENTED reality ,WIND turbines ,RENEWABLE energy sources ,WIND power ,WIND power plants ,MAINTENANCE costs ,AUTOMATION - Abstract
To achieve the goal of net zero emissions by 2050, Taiwan's government is committed to aggressively developing renewable energy sources, with wind power being among the top three. However, when wind turbines experience failures, it is common to shut them down and call for professional help to troubleshoot, leading to significant losses due to transportation costs and downtime. Traditional maintenance methods rely on expertise and are limited by paper manuals and screen-based tutorials. As Industry 4.0 progresses, IoT technology is being increasingly adopted across industries, leading to greater automation. Augmented reality (AR) technology is also gaining recognition as science and technology rapidly advance. AR technology adds virtual objects to the physical world and presents the potential to simplify tasks that once required significant time and effort. Additionally, it provides real-time information to technicians and has become an indispensable auxiliary system in various sectors. In this study, we aim to develop an AR-based, interactive, and real-time wind turbine maintenance auxiliary platform system using Unity3D and Vuforia. The system can control and repair wind turbines, monitor operational data and conditions in real time, and alert users to maintenance and repair tasks in the event of failures. This AR maintenance assistance platform also includes a remote expert system. When users are unable to troubleshoot issues themselves, they can connect with remote experienced experts for support, increasing wind farm efficiency and enabling real-time monitoring. The presented AR system can assist operations and maintenance (O&M) of a wind farm in reducing the maintenance cost and time, and thus increase the efficiency and output yield of the wind farm. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
6. Effects of secondary reaction of primary volatiles on oil/gas yield and quality in oil shale pyrolysis
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Yu-ming Zhang, Pei Qiao, Jun-tao Guan, Jia-zhou Li, and Wei Zhang
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business.industry ,Fossil fuel ,complex mixtures ,law.invention ,chemistry.chemical_compound ,Diesel fuel ,Chemical engineering ,chemistry ,law ,Yield (chemistry) ,Pyrolysis oil ,Gasoline ,business ,Distillation ,Pyrolysis ,Oil shale - Abstract
The reaction behaviors of primary volatiles from oil shale pyrolysis were investigated using a two-stage reactor under different in-situ thermal reaction conditions. The reaction parameters of secondary reactor, such as, reactor temperature, atmosphere and residence time, were studied for their effects on the pyrolysis oil/gas yield and quality. The results showed that the reaction temperature had profound influence on the oil and gas yield. The pyrolyzed shale oil and gas yield reduced by 15% and increased by 20% (mass) respectively, with the temperature of 2nd stage reactor increasing from 600 to 650°C under optimized reaction condition of 1st stage. Comparing with nitrogen atmosphere, the liquid oil yield could be enhanced by 5% when steam was added as the reaction atmosphere in the second stage, and the corresponding oil was mainly concentrated in the gasoline and diesel distillations (that is, boiling point
- Published
- 2021
7. Degradation of indole via a two-component indole oxygenase system from Enterococcus hirae GDIAS-5
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Jun-Jin Deng, Jing-Yi Hu, Xue-ying Han, Yang Li, Xiao-Chun Luo, Zhi-Lin Wang, and Jia-Zhou Li
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Environmental Engineering ,Health, Toxicology and Mutagenesis ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Published
- 2023
8. Promotion of feather waste recycling by enhancing the reducing power and keratinase activity of Streptomyces sp. SCUT-3
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Jia-Zhou Li, Xiao-Chun Luo, Liang Shuang, Ming-Shu Zhang, Mao Hehua, Jun-Jin Deng, Zi-Yang Luo, Wen-Jun Lu, De-Lin Lu, and Zhi-Wei Li
- Subjects
0301 basic medicine ,animal structures ,Bioconversion ,medicine.medical_treatment ,01 natural sciences ,Streptomyces ,03 medical and health sciences ,chemistry.chemical_compound ,Sulfite ,medicine ,Environmental Chemistry ,Food science ,Protease ,biology ,010405 organic chemistry ,Chemistry ,biology.organism_classification ,Pollution ,0104 chemical sciences ,030104 developmental biology ,Keratinase ,Feather ,visual_art ,visual_art.visual_art_medium ,biology.protein ,Fermentation ,Bacteria - Abstract
As a potential renewable and nitrogen-rich resource, millions of tons of feather waste are generated from poultry farming and this has been steadily increasing. Bioconversion is the most promising low-cost and environmentally benign recycling method, while the efficiency of the isolated natural feather degrading bacteria (FDB) cannot satisfy the requirements of industrial utilization. The process of bacterial degradation of feathers not only requires proteases but also involves complex reduction mechanisms that have not yet been revealed. Previous heterologous expression of keratinase genes in non-FDB hosts achieved only very limited success. In this study, the mechanism of sulfite production via cysteine dioxygenase (CDO1) oxidation was first identified in Streptomyces sp. SCUT-3, which is important for feather disulfide bond reduction. The knockdown of cdo1 significantly decreased the sulfite production (from 38.5 to 22.5 mg L−1) and feather degradation rate (from 45.7% to 39.5%) of SCUT-3 in 5% chicken feather medium (CFM) culture. The overexpression of cdo1 increased bacterial single-cell sulfite production and feather protein conversion efficiency by 3.8 and 2.5 fold, respectively, on day 2 in 5% CFM. On this basis, a new strategy is proposed for increasing feather degradation efficiency by enhancing the reducing power and keratinase activity of FDB according to their own feather degradation mechanism. Thus, the co-overexpression of CDO1 and protease Sep39 successfully increased SCUT-3's feather protein conversion efficiency (42.5% higher than the wild-type) on day 2 in 5% CFM culture. With the co-overexpression strain SCUT-Ocdo1-sep39, 57.5% of proteins in feathers were converted to soluble peptides and free amino acids (0.20 g and 0.29 g g−1 feather, respectively) by solid-state fermentation in 6 d, which was 28.6% higher than that obtained for wild-type SCUT-3. The method developed herein exhibits a much higher economic benefit and a lower ecological footprint than previously reported feather-processing methods and is powerful for industrial recycling of feather waste. This study also elucidates FDB's disulfide bond mechanism and provides a reference for further genetic modification of FDB.
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- 2021
9. Recombinant neutral protease rNpI as fish feed additive to improve protein digestion and growth
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Jia-Zhou Li, Zhong‐Qiu Li, Jun-Jin Deng, Min Zhao, Shun Xu, Xiao-Chun Luo, De-Lin Lu, Dan Shi, and Zi-Juan You
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0303 health sciences ,Proteases ,Rapeseed ,Protease ,Protein digestion ,medicine.medical_treatment ,04 agricultural and veterinary sciences ,Aquatic Science ,Biology ,Commercial fish feed ,03 medical and health sciences ,Papain ,chemistry.chemical_compound ,chemistry ,Plant protein ,040102 fisheries ,medicine ,0401 agriculture, forestry, and fisheries ,Food science ,Digestion ,030304 developmental biology - Abstract
The addition of heavy protein to aquafeed causes low digestibility, high cost and significant nitrogen pollution in the water. The addition of protease is an effective way of improving protein absorption, but few proteases have been developed specifically for aquafeeds. The hydrolysis efficiency of protease additives for plant protein in the neutral gut environment and their synergy with endogenous proteases has not been sufficiently investigated. In this study, the performance of the recombinant neutral protease rNpI as an aquafeed additive to reduce the raw protein materials in the diet of Carassius carassius during 56 days of growth was evaluated. rNpI effectively hydrolysed soya bean protein isolates (SPI) with a high degree of hydrolysation and a high small‐peptide yield. The rNpI‐treated SPI exhibited a notable antioxidant activity, which benefited fish health. In vitro digestibility indicated that rNpI complemented trypsin in SPI digestion better than papain or Alcalase. The growth trial showed that the addition of rNpI could warrant a reduction in the crude protein and gross energy of feed while producing a lower feed‐conversion rate (FCR) and better growth performance. Crucian that were fed an RM‐wheat‐E diet (5.5% rapeseed meal and 5% wheat reduction) showed the best performance, with the lowest FCR and highest weight gain. These results indicate that rNpI could be an effective additive for the aquafeed industry and could provide economic benefits by reducing aquafeed protein requirements and nitrogen emissions. This study provides novel information and strategies for additive protease use and development in aquafeed.
- Published
- 2020
10. Distribution of Mpeg1+ cells in healthy grouper (Epinephelus coioides) and after Cryptocaryon irritans infection
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Jia-Zhou Li, Yan-Wei Li, Rui Han, An-Xing Li, Lu-Yun Ni, Hong-Ping Chen, Xue-Ming Dan, and Xiao-Chun Luo
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0301 basic medicine ,Head Kidney ,biology ,Cryptocaryon ,medicine.diagnostic_test ,Spleen ,04 agricultural and veterinary sciences ,General Medicine ,Aquatic Science ,biology.organism_classification ,Immunofluorescence ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,Immune system ,medicine.anatomical_structure ,Polyclonal antibodies ,040102 fisheries ,medicine ,biology.protein ,0401 agriculture, forestry, and fisheries ,Environmental Chemistry ,Macrophage ,Grouper - Abstract
Cryptocaryon irritans is an extremely harmful ciliated obligate parasite that is responsible for large economic losses in aquaculture. C. irritans infection can cause an insect-resistant immune response in fish, and many immune cells can be observed in the local infection site. However, it is unclear whether macrophages are involved in the host defense against C. irritans infection. The Mpeg1 protein can form pores and destroy the cell membrane of invading pathogens, and is also used as a macrophage-specific marker in mammals. Therefore, a polyclonal antibody against grouper recombinant Mpeg1a was produced to mark macrophages in this study, which could recognize both isoforms of Mpeg1 (Mpeg1a/b). Immunofluorescence revealed that EcMpeg1 positive cells were mostly distributed in the head kidney and spleen in healthy grouper. Immunofluorescence and immunohistochemistry showed that the number of EcMpeg1 positive cells increased in the gills after infection with C. irritans, implying that EcMpeg1 positive cells may be involved in the process of grouper resistance against C. irritans infection.
- Published
- 2020
11. One-step processing of shrimp shell waste with a chitinase fused to a carbohydrate-binding module
- Author
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Jun-Jin Deng, Ming-Jun Zhu, Zhi-Wei Li, Jia-Zhou Li, Xiao-Chun Luo, Ming-Shu Zhang, He-Hua Mao, and De-Lin Lu
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0303 health sciences ,biology ,010405 organic chemistry ,Chemistry ,Bioconversion ,fungi ,01 natural sciences ,Pollution ,Hydrolysate ,0104 chemical sciences ,Shrimp ,03 medical and health sciences ,Hydrolysis ,chemistry.chemical_compound ,Chitin ,Enzymatic hydrolysis ,Chitinase ,biology.protein ,Environmental Chemistry ,Carbohydrate-binding module ,Food science ,030304 developmental biology - Abstract
As a potential renewable and nitrogen-rich feedstock, tons of shrimp shell waste is generated from the increasing consumption of seafood. However, its traditional processing is costly and creates pollution. The bioconversion of shell waste into valuable nitrogen-containing chemicals via enzymatic hydrolysis is a promising technology. However, intact shells are poorly hydrolyzed by chitinases unless pre-demineralization and deproteinization with chemicals or proteases are performed. In this study, three carbohydrate-binding modules (CBMs) were fused to the C-terminal of a chitinase Chit46 to enhance its hydrolysis of shrimp shell waste. The addition of CBMs significantly improved the chitinase activity and substrate-binding activity of Chit46. Chimeric chitinases could directly hydrolyze shrimp shells without pretreatment and their hydrolysis efficiency was much higher than that of previously reported chitinases. With the modified chitinase (‘Chit46-CBM3’), we developed a one-step shrimp shell processing method. The method could convert 46.5% of chitin in shrimp shells to chitin oligomers by hydrolysis in 12 h. Partial protein release accompanied chitin hydrolysis. Consisting of 8.8 g l−1 chitin oligomers and 11.3 g l−1 protein, the hydrolysate could support robust microbial growth and the residue was more digestible by mammals than conventional shrimp shell powder. This process is superior to previously reported methods in cost, conversion efficiency and labour. The one-step process also exhibited a much lower ecological footprint than conventional processing and can be applied to other types of chitinous waste in addition to shrimp shells.
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- 2020
12. Indole metabolism mechanisms in a new, efficient indole-degrading facultative anaerobe isolate Enterococcus hirae GDIAS-5
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Jun-Jin Deng, Dun Deng, Zhi-Lin Wang, Xiao-Chun Luo, Hong-Ping Chen, Shu-Yang Liu, Xian-Yong Ma, and Jia-Zhou Li
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Isatin ,Enterococcus hirae ,Indoles ,Environmental Engineering ,Bacteria ,Health, Toxicology and Mutagenesis ,Catechols ,Environmental Chemistry ,Ferric Compounds ,Pollution ,Waste Management and Disposal ,Oxindoles - Abstract
Indole is an inter-species and inter-kingdom signaling molecule widespread in the natural world. A large amount of indole in livestock wastes makes it difficult to be degraded, which causes serious malodor. Identifying efficient and eco-friendly ways to eliminate it is an urgent task for the sustainable development of husbandry. While bioconversion is a widely accepted means, the mechanism of indole microbial degradation is little understood, especially under anaerobic conditions. Herein, a new Enterococcus hirae isolate GDIAS-5, effectively degraded 100 mg/L indole within 28 h aerobically or 5 days anaerobically. Three intermediates (oxindole, isatin, and catechol) were identified in indole degradation, and catechol was further degraded by a meta-cleavage catabolic pathway. Two important processes for GDIAS-5 indole utilization were discovered. One is Fe(III) uptake and reduction, which may be a critical process that is coupled with indole oxidation, and the other is the entire pathway directly involved in indole oxidation and metabolism. Furthermore, monooxygenase ycnE responsible for indole oxidation via the indole-oxindole-isatin pathway was identified for the first time. Bioinformatic analyses showed that ycnE from E. hirae formed a phylogenetically separate branch from monooxygenases of other species. These findings provide new targets and strategies for synthetic biological reconstruction of indole-degrading bacteria.
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- 2022
13. Marinobacter shengliensis subsp. alexandrii Subsp. Nov., Isolated from Cultivable Phycosphere Microbiota of Highly Toxic Dinoflagellate Alexandrium catenella LZT09 and Description of Marinobacter shengliensis Subsp. shengliensis Subsp. Nov
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Jia-Zhou Li, Nurhezreen Md Iqbal, Shen-Quan Liao, Rong Xiang, Xi Yang, Lin-Zeng Yu, Qiao Yang, Xiao-Ai Zhang, Chun-Tian Zheng, Lei Wang, Ming-Fei Sun, and Yu-Han Duan
- Subjects
DNA, Bacterial ,Alexandrium catenella ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,Phylogenetics ,RNA, Ribosomal, 16S ,Marinobacter ,medicine ,Paralytic shellfish poisoning ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Base Composition ,biology ,030306 microbiology ,Microbiota ,Fatty Acids ,Dinoflagellate ,Nucleic Acid Hybridization ,General Medicine ,Sequence Analysis, DNA ,Ribosomal RNA ,biology.organism_classification ,16S ribosomal RNA ,medicine.disease ,Bacterial Typing Techniques ,Dinoflagellida ,Marinobacter shengliensis - Abstract
Phycosphere hosts the boundary of unique holobionts harboring dynamic algae-bacteria interactions. During our investigating the microbial consortia composition of phycosphere microbiota (PM) derived from diverse harmful algal blooms (HAB) dinoflagellates, a novel rod-shaped, motile and faint yellow-pigmented bacterium, designated as strain LZ-6 T, was isolated from HAB Alexandrium catenella LZT09 which produces high levels paralytic shellfish poisoning toxins. Phylogenetic analysis based on 16S rRNA gene and two housekeeping genes, rpoA and pheS sequences showed that the novel isolate shared the highest gene similarity with Marinobacter shengliensis CGMCC 1.12758 T (99.6%) with the similarity values of 99.6%, 99.9% and 98.5%, respectively. Further phylogenomic calculations of average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between strains LZ-6 T and the type strain of M. shengliensis were 95.9%, 96.4% and 68.5%, respectively. However, combined phenotypic and chemotaxonomic characterizations revealed that the new isolate was obviously different from the type strain of M. shengliensis. The obtained taxonomic evidences supported that strain LZ-6 T represents a novel subspecies of M. shengliensis, for which the name is proposed, Marinobacter shengliensis subsp. alexandrii subsp. nov. with the type strain LZ-6 T (= CCTCC AB 2018388TT = KCTC 72197 T). This proposal automatically creates Marinobacter shengliensis subsp. shengliensis for which the type strain is SL013A34A2T (= LMG 27740 T = CGMCC 1.12758 T).
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- 2020
14. Role of major histocompatibility complex II antigen-presentation pathway genes in orange-spotted grouper infected with Cryptocaryon irritans
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Jia-Zhou Li, Dong-Dong Xu, Ze-Quan Mo, Xiao-Chun Luo, Jun-Jin Deng, Xue-Ming Dan, Shun Xu, and Yan-Wei Li
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0301 basic medicine ,Fish Proteins ,Orange-spotted grouper ,Veterinary (miscellaneous) ,Antigen presentation ,chemical and pharmacologic phenomena ,Spleen ,Antigens, Protozoan ,Ciliophora Infections ,Aquatic Science ,Major histocompatibility complex ,Microbiology ,Major Histocompatibility Complex ,03 medical and health sciences ,Fish Diseases ,Immune system ,Antigen ,medicine ,Animals ,Grouper ,Immunity, Mucosal ,Hymenostomatida ,biology ,04 agricultural and veterinary sciences ,biology.organism_classification ,Acquired immune system ,Immunity, Humoral ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Bass - Abstract
Cryptocaryon irritans, a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C. irritans remain unknown. In this study, the full-length cDNA of the grouper cysteine protease CTSS was cloned. The expression distributions of six genes (CTSB, CTSL, CTSS, GILT, MHC IIA and MHC IIB) involved in MHC II antigen presentation pathway were tested. These genes were highly expressed in systematic immune tissues and skin and gill mucosal-associated immune tissues. All six genes were upregulated in skin at most time points. Five genes expected CTSS was upregulated in spleen at most time points. CTSB, CTSL and MHC IIA were upregulated in the gill and head kidney at some time points. These results indicate that the presentation of MHC II antigen intensively occurred in local infected skin and gill. Spleen, not head kidney, had the most extensive systematic antigen presentation. In skin, six genes most likely peaked at day 2, earlier than in spleen (5-7 days), marking an earlier skin antibody peak than any recorded in serum previously. This significant and earlier mucosal antigen presentation indicates that specific immune response occurs in local mucosal tissues.
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- 2020
15. Distribution of Mpeg1
- Author
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Lu-Yun, Ni, Hong-Ping, Chen, Rui, Han, Xiao-Chun, Luo, An-Xing, Li, Jia-Zhou, Li, Xue-Ming, Dan, and Yan-Wei, Li
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Fish Proteins ,Gills ,Fish Diseases ,Macrophages ,Animals ,Membrane Proteins ,Ciliophora Infections ,Ciliophora ,Disease Resistance ,Perciformes - Abstract
Cryptocaryon irritans is an extremely harmful ciliated obligate parasite that is responsible for large economic losses in aquaculture. C. irritans infection can cause an insect-resistant immune response in fish, and many immune cells can be observed in the local infection site. However, it is unclear whether macrophages are involved in the host defense against C. irritans infection. The Mpeg1 protein can form pores and destroy the cell membrane of invading pathogens, and is also used as a macrophage-specific marker in mammals. Therefore, a polyclonal antibody against grouper recombinant Mpeg1a was produced to mark macrophages in this study, which could recognize both isoforms of Mpeg1 (Mpeg1a/b). Immunofluorescence revealed that EcMpeg1 positive cells were mostly distributed in the head kidney and spleen in healthy grouper. Immunofluorescence and immunohistochemistry showed that the number of EcMpeg1 positive cells increased in the gills after infection with C. irritans, implying that EcMpeg1 positive cells may be involved in the process of grouper resistance against C. irritans infection.
- Published
- 2020
16. The feather degradation mechanisms of a new Streptomyces sp. isolate SCUT-3
- Author
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Ming-Shu Zhang, Jun-Jin Deng, Jia-Zhou Li, Liang Shuang, Zhi-Wei Li, De-Lin Lu, Xiao-Chun Luo, and Ye Ke
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animal structures ,Bioconversion ,Microorganism ,medicine.medical_treatment ,Medicine (miscellaneous) ,Streptomyces ,General Biochemistry, Genetics and Molecular Biology ,Article ,Substrate Specificity ,Avian Proteins ,Applied microbiology ,03 medical and health sciences ,Bacterial Proteins ,medicine ,Animals ,Food science ,lcsh:QH301-705.5 ,030304 developmental biology ,Waste Products ,0303 health sciences ,Protease ,biology ,030306 microbiology ,food and beverages ,Feathers ,beta-Keratins ,biology.organism_classification ,Biodegradation, Environmental ,Keratinase ,lcsh:Biology (General) ,Feather ,visual_art ,Proteolysis ,visual_art.visual_art_medium ,biology.protein ,Biocatalysis ,Fermentation ,General Agricultural and Biological Sciences ,Bacteria ,Peptide Hydrolases - Abstract
Feather waste is the highest protein-containing resource in nature and is poorly reused. Bioconversion is widely accepted as a low-cost and environmentally benign process, but limited by the availability of safe and highly efficient feather degrading bacteria (FDB) for its industrial-scale fermentation. Excessive focuses on keratinase and limited knowledge of other factors have hindered complete understanding of the mechanisms employed by FDB to utilize feathers and feather cycling in the biosphere. Streptomyces sp. SCUT-3 can efficiently degrade feather to products with high amino acid content, useful as a nutrition source for animals, plants and microorganisms. Using multiple omics and other techniques, we reveal how SCUT-3 turns on its feather utilization machinery, including its colonization, reducing agent and protease secretion, peptide/amino acid importation and metabolism, oxygen consumption and iron uptake, spore formation and resuscitation, and so on. This study would shed light on the feather utilization mechanisms of FDBs., Li et a. report a new Streptromyces isolate, SCUT-3 which can efficiently degrade feather into products with high amino acid content, useful as feed for plants, animals and microbes. Using multiple omics and other techniques, they report how SCUT-3 turns on its feather utilization machinery and suggest a number of expressed genes most likely implicated in feather degradation.
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- 2019
17. Effects of antimicrobial peptides on serum biochemical parameters, antioxidant activity and non-specific immune responses in Epinephelus coioides
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Yan-Wei Li, Jia-Zhou Li, Youhua Huang, Gang Wang, Liang-Shi Chen, Jia-Yang He, Guo Chen, Qiwei Qin, Muting Yan, Hongyan Sun, Yu-Ling Su, Xue-Ming Dan, and Tian-Yong Zhan
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0301 basic medicine ,Antioxidant ,Lipopolysaccharide ,medicine.medical_treatment ,Antimicrobial peptides ,Aquatic Science ,Antioxidants ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,Immune system ,medicine ,Environmental Chemistry ,Animals ,biology ,Albumin ,04 agricultural and veterinary sciences ,General Medicine ,Antimicrobial ,Animal Feed ,Immunity, Innate ,Diet ,030104 developmental biology ,chemistry ,Biochemistry ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Bass ,Lysozyme ,Blood Chemical Analysis ,Antimicrobial Cationic Peptides ,Bacillus subtilis - Abstract
Antimicrobial peptides (AMPs) are small proteins showing broad-spectrum antimicrobial activity that have been known to be powerful agents against a variety of pathogens (bacteria, fungi and viruses). In this study, the effects of AMPs from Bacillus subtilis on Epinephelus coioides were examined. E. coioides were fed with diets containing AMPs (0, 100, 200, 400 or 800 mg/kg) for four weeks. Results showed that the levels of total protein (TP), albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and blood glucose (GLU) and lipopolysaccharide (LPS) in the serum of E. coioides changed than those of the control group; compared to the control group, the levels of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and lysozyme (LZM) levels in E. coioides fed with different dosages AMP diets were also different; in addition, the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin-1-beta (IL-1β), and heat shock protein 90 (Hsp90) in the tissues of E. coioides were measured, the three genes in the tissues examined were significantly upregulated. The results demonstrated that diets containing AMPs can enhance the antioxidant capacity and innate immune ability of E. coioides, indicating that AMPs might be a potential alternative to antibiotics in E. coioides.
- Published
- 2018
18. PD-1 inhibitor plus anlotinib for metastatic castration-resistant prostate cancer: a real-world study
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Xin-Xing Du, Yan-Hao Dong, Han-Jing Zhu, Xiao-Chen Fei, Yi-Ming Gong, Bin-Bin Xia, Fan Wu, Jia-Yi Wang, Jia-Zhou Liu, Lian-Cheng Fan, Yan-Qing Wang, Liang Dong, Yin-Jie Zhu, Jia-Hua Pan, Bai-Jun Dong, and Wei Xue
- Subjects
anlotinib ,ctdna ,immune checkpoint inhibitor ,programmed cell death-1 inhibitor ,prostate cancer ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Management and treatment of terminal metastatic castration-resistant prostate cancer (mCRPC) remains heavily debated. We sought to investigate the efficacy of programmed cell death 1 (PD-1) inhibitor plus anlotinib as a potential solution for terminal mCRPC and further evaluate the association of genomic characteristics with efficacy outcomes. We conducted a retrospective real-world study of 25 mCRPC patients who received PD-1 inhibitor plus anlotinib after the progression to standard treatments. The clinical information was extracted from the electronic medical records and 22 patients had targeted circulating tumor DNA (ctDNA) next-generation sequencing. Statistical analysis showed that 6 (24.0%) patients experienced prostate-specific antigen (PSA) response and 11 (44.0%) patients experienced PSA reduction. The relationship between ctDNA findings and outcomes was also analyzed. DNA-damage repair (DDR) pathways and homologous recombination repair (HRR) pathway defects indicated a comparatively longer PSA-progression-free survival (PSA-PFS; 2.5 months vs 1.2 months, P = 0.027; 3.3 months vs 1.2 months, P = 0.017; respectively). This study introduces the PD-1 inhibitor plus anlotinib as a late-line therapeutic strategy for terminal mCRPC. PD-1 inhibitor plus anlotinib may be a new treatment choice for terminal mCRPC patients with DDR or HRR pathway defects and requires further investigation.
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- 2023
- Full Text
- View/download PDF
19. Development and Prospect of Genetically Engineered Pharmaceutics
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Ya Qiong Huang, Shi Hua Zhao, Shi Yuan Zeng, Jia Zhou Li, Xu Jian Chen, De Shun Shi, Gui Wen Ruan, and Kenneth L. White
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Engineering ,Genetically engineered ,business.industry ,General Engineering ,Pharmaceutics ,Nanotechnology ,Computational biology ,Health outcomes ,business ,Human being ,Genetic therapy ,DNA shuffling - Abstract
With the rapid development of modern biology and technology, Genetically Engineered Pharmaceutics has become the most important field in modern medicinal industry. Currently, we have taken concern that the Genetically Engineered Pharmaceutics has been a subject of research. As advanced genetically engineered technology, genetic therapy, the point mutation techniques, DNA shuffling, RNA interference (RNAi) technology, the bioinformatics for genome and proteome analyses, the high throughput screening technology, etc. can be applied in the treatment of cancer, and other targeted therapies. A great deal of advanced technologies accelerates the development of genetically engineered pharmaceutics, and plays a key role in genetically engineered pharmaceutics. With the advances in genetically engineered pharmaceutics, we look forward to finding more new drugs, such as the high-value peptides, small functional molecular groups, proteins, especially antibodies, vaccines, enzymes, and therapeutic peptides. These developments continue to secure greater health outcomes of human being at all aspects in the long run. In this article, the development and prospect of Genetically Engineered Pharmaceutics are reviewed, and furthermore, the potential development and huge prospects are also presented.
- Published
- 2013
20. Culture Conditions affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase
- Author
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Yuanyuan Zhang, Mingquan Xie, Jia-zhou Li, and Xiao-Chun Luo
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chemistry.chemical_classification ,Recombinant escherichia coli ,food.ingredient ,Food additive ,Transporter ,General Chemistry ,Biology ,biology.organism_classification ,Industrial and Manufacturing Engineering ,Enzyme ,food ,Biochemistry ,chemistry ,Side chain ,Gene ,Decaprenyl diphosphate synthase ,Bacteria ,Food Science - Abstract
Ubiquinones (UQ) are important electron transporters and play lot of important roles in most organisms. In different species, UQ was classified to be UQ-6, 7, 8, 9, 10 according to their polyprenyl side chain length. The side chain's length is determined by the enzyme named Poly-Prenyl diphosphate synthases (PPPS). Bacteria are usually reconstructed to producing UQ-10 used in human's food additive, medicine or cosmetics, such as using decaprenyl Diphosphate Synthase (DPS) gene from R. radiobacter to substitute E.coli's octaprenyl diphosphate synthase gene , just like E. coli BL21 (�� ispB::ddsA) used in this study. It is interesting that not only in these reconstructed bacteria, but in human-being, DPS can synthesize UQ-9 besides UQ-10. The mechanism of this phenomenon is still unknown. In this study, the effects of culture conditions, including the temperature, dissolved oxygen, pH and culture medium, on the DPS characteristics in E. coli BL21 (�� ispB::ddsA) were examined. Results show that temperature greatly affects the ratio of UQ-9/UQ-10, but not the total ubiquinone's production. Increasing dissolved oxygen and protein concentration in culture medium can promote total ubiquinone's production, but not the ratio of UQ-9/UQ-10. These results may give reference for UQ-10's industrial produce and the mechanism of these conditions' effect on DPS will be discussed.
- Published
- 2013
21. Enzymatic Characteristics of a Recombinant Neutral Protease I (rNpI) from Aspergillus oryzae Expressed in Pichia pastoris
- Author
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Xiao-Chun Luo, Ye Ke, Mingquan Xie, Jia-zhou Li, and Wei-Qian Huang
- Subjects
Hydrolyzed protein ,Arachis ,Aspergillus oryzae ,Molecular Sequence Data ,Peptide ,Pichia ,Substrate Specificity ,Pichia pastoris ,Fungal Proteins ,chemistry.chemical_compound ,Hydrolysis ,Insulin ,Cloning, Molecular ,chemistry.chemical_classification ,Base Sequence ,biology ,Temperature ,Metalloendopeptidases ,General Chemistry ,Hydrogen-Ion Concentration ,biology.organism_classification ,Recombinant Proteins ,Amino acid ,Papain ,Enzyme ,Gene Expression Regulation ,chemistry ,Biochemistry ,Mutagenesis, Site-Directed ,Soybean Proteins ,General Agricultural and Biological Sciences ,Oxidation-Reduction - Abstract
A truncated neutral protease I (NpI) from Aspergillus oryzae 3.042 was expressed in Pichia pastoris with a high enzyme yield of 43101 U/mL. Its optimum pH was about 8.0, and it was stable in the pH range of 5.0-9.0. Its optimum temperature was about 55 °C and retained90% activity at 50 °C for 120 min. Recombinant NpI (rNpI) was inhibited by Cu(2+) and EDTA. Eight cleavage sites of rNpI in oxidized insulin B-chain were determined by mass spectrometry, and five of them had high hydrophobic amino acid affinity, which makes it efficient in producing antihypertensive peptide IPP from β-casein and a potential debittering agent. The high degree of hydrolysis (DH) of rNpI to soybean protein (8.8%) and peanut protein (11.1%) compared to papain and alcalase makes it a good candidate in the processing of oil industry byproducts. The mutagenesis of H(429), H(433), and E(453) in the deduced zinc-binding motif confirmed rNpI as a gluzincin. All of these results show the great potential of rNpI to be used in the protein hydrolysis industry.
- Published
- 2012
22. The Structure and Enzyme Characteristics of a Recombinant Leucine Aminopeptidase rLap1 from Aspergillus sojae and Its Application in Debittering
- Author
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Jia-zhou Li, Xiao-Chun Luo, Zhi-Zhong Meng, Wei-Qian Huang, Li-Fen Zhong, and Zi-Juan You
- Subjects
Models, Molecular ,Genetic Vectors ,Molecular Sequence Data ,Bioengineering ,Biology ,Aspergillus sojae ,Applied Microbiology and Biotechnology ,Biochemistry ,Aminopeptidase ,Hydrolysate ,Pichia pastoris ,Substrate Specificity ,Leucyl Aminopeptidase ,Casein ,Enzyme Stability ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,chemistry.chemical_classification ,Hydrolysis ,Temperature ,Substrate (chemistry) ,General Medicine ,Hydrogen-Ion Concentration ,biology.organism_classification ,Recombinant Proteins ,Amino acid ,Aspergillus ,chemistry ,Structural Homology, Protein ,Taste ,Electrophoresis, Polyacrylamide Gel ,Leucine ,Hydrophobic and Hydrophilic Interactions ,Sequence Alignment ,Biotechnology - Abstract
A leucine aminopeptidase Lap1 was cloned from Aspergillus sojae GIM3.30. The truncated Lap1 without a signal peptide was over-expressed in P. pastoris, and the enzymatic characteristics of recombinant Lap1 (rLap1) were tested. The rLap1 was about 36.7 kDa with an optimal pH 8.0 and optimal temperature 50 °C for substrate Leu-p-nitroanilide and it sustained 50 % activity after 1 h incubation at 50 °C. The activity of rLap1 was significantly inhibited by EDTA, whereas Co(2+), Mn(2+), and Ca(2+) ions, but not Zn(2+) ions, restored its activity. rLap1 showed the highest activity against Arg-pNA and then Leu-, Lys-, Met-, and Phe-pNA. The 3D structure of rLap1 showed it had a conserved functional charge/dipole complex and a hydrogen bond network of Zn2-D179-S228-Q177-D229-S158 around its active center. An acidic Asp residue was found at the bottom of the substrate binding pocket, which explains its preference for basic N-terminal amino acid substrates such as Arg and Lys. rLap1 improved the degree of hydrolysis of casein and soy protein hydrolysates and also decreased their bitterness, indicating its potential utility in food production.
- Published
- 2015
23. Frequency-multiplying optoelectronic oscillator with a tunable frequency multiplication technique
- Author
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Liao Jun, Tian-long Yang, Chang Chen, Cheng-xin Li, Gan Xiaqing, Wei Wang, Chen Tailong, Jia-zhou Li, and Xin Kang
- Subjects
Optical fiber ,Materials science ,business.industry ,Optoelectronic oscillator ,General Engineering ,02 engineering and technology ,Atomic and Molecular Physics, and Optics ,law.invention ,020210 optoelectronics & photonics ,Optics ,Band-pass filter ,law ,0202 electrical engineering, electronic engineering, information engineering ,Optoelectronics ,Multiplication ,business ,Linear filter - Published
- 2016
24. [Study on chemical constituents of tress of Toona ciliata]
- Author
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Jia-Zhou, Li, Hua-Nong, Mo, and Xian-Mao, Ning
- Subjects
Plant Leaves ,Plants, Medicinal ,Molecular Structure ,Plant Extracts ,Palmitic Acid ,Stigmasterol ,Meliaceae ,Sitosterols - Abstract
To study the chemical constituents of the tress of Toona ciliata.The compounds were isolated by many kinds of chromatography methods and identified on the basis of physico-chemical characters and spectroscopic analysis.Seven compounds were obtained from the petrol and chloroform extracts of the tress of Toona ciliata, and their structures were identified as 3-Acetoxy-17-furan-3-yl-1-hydroxy-1, 4, 4, 10, 13-pentamethyl-12-oxo-tetradecahydro-16, 20-dioxa-cyclopropa [14, 15] cyclopenta [alpha] phenanthrene-7-carboxylic acid methyl ester (I), beta-sitosterol (II), stigmasterol (III), n-C35H72 (IV), palmitinic acid (V), n-C20H42 (VI), 3-(3-Propyl-[1,1',3',1"]tercyclohexan-3"-yl)-propan-1-ol (VII).Compounds I, IV, VI, VII are isolated from this plant for the first time and compound I is a new compound.
- Published
- 2010
25. Frequency-multiplying optoelectronic oscillator with a tunable frequency multiplication technique.
- Author
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Cheng-xin Li, Wei Wang, Chang Chen, Tai-long Chen, Xia-qing Gan, Jun Liao, Tian-long Yang, Jia-zhou Li, and Xin Kang
- Subjects
OPTOELECTRONIC devices ,PHOTODETECTORS ,MICROWAVE photonics - Abstract
An optoelectronic oscillator (OEO) for the generation of a frequency-doubled or -quadrupled microwave signal with a tunable frequency multiplication technique is proposed and demonstrated. In the proposed OEO, a modulated signal is generated by a Mach-Zehnder modulator (MZM). The modulated signal is then divided into two parts. One part is sent to a photodetector (PD1) via an optical domain dual-loop composite cavity and then fed back to the MZM to form the OEO loop that can generate an oscillation signal with the frequency determined by the center frequency of an electrical bandpass filter used in the loop and the length difference of the dual-loop composite cavity. The other part is sent to a fiber-optic recirculating delay line (RDL) structure and then applied to another PD (PD2) for the formation of a microwave photonic (MWP) notch filter. Through using the cascade characteristics of the RDL loop in the fiber-optic RDL structure and the notch response of the MWP notch filter, a frequency-doubled or -quadrupled microwave signal is generated. A detailed theoretical analysis is provided and the results are verified by an experiment. A fundamental oscillation signal at 5.592 GHz is generated in the OEO loop, which is multiplied to generate a frequency-doubled microwave signal at 11.184 GHz or a frequency-quadrupled microwave signal at 22.368 GHz, respectively. The performance of the generated microwave signals is also investigated. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
26. BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer
- Author
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Jia-Zhou Liu, Yi-Lin Hu, Ying Feng, Yun Jiang, Yi-Bing Guo, Yi-Fei Liu, Xi Chen, Jun-Ling Yang, Yu-yan Chen, Qin-Sheng Mao, and Wan-Jiang Xue
- Subjects
BDH2 ,Nrf2 ,Gastric cancer ,ROS ,PI3K ,Autophagy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background 3-Hydroxy butyrate dehydrogenase 2 (BDH2) is a short-chain dehydrogenase/reductase family member that plays a key role in the development and pathogenesis of human cancers. However, the role of BDH2 in gastric cancer (GC) remains largely unclear. Our study aimed to ascertain the regulatory mechanisms of BDH2 in GC, which could be used to develop new therapeutic strategies. Methods Western blotting, immunohistochemistry, and RT-PCR were used to investigate the expression of BDH2 in GC specimens and cell lines. Its correlation with the clinicopathological characteristics and prognosis of GC patients was analysed. Functional assays, such as CCK-8 and TUNEL assays, transmission electron microscopy, and an in vivo tumour growth assay, were performed to examine the proliferation, apoptosis, and autophagy of GC cells. Related molecular mechanisms were clarified by luciferase reporter, coimmunoprecipitation, and ubiquitination assays. Results BDH2 was markedly downregulated in GC tissues and cells, and the low expression of BDH2 was associated with poor survival of GC patients. Functionally, BDH2 overexpression significantly induced apoptosis and autophagy in vitro and in vivo. Mechanistically, BDH2 promoted Keap1 interaction with Nrf2 to increase the ubiquitination level of Nrf2. Ubiquitination/degradation of Nrf2 inhibited the activity of ARE to increase accumulation of reactive oxygen species (ROS), thereby inhibiting the phosphorylation levels of AktSer473 and mTORSer2448. Conclusions Our study indicates that BDH2 is an important tumour suppressor in GC. BDH2 regulates intracellular ROS levels to mediate the PI3K/Akt/mTOR pathway through Keap1/Nrf2/ARE signalling, thereby inhibiting the growth of GC.
- Published
- 2020
- Full Text
- View/download PDF
27. Neoadjuvant Chemotherapy in Early Triple-Negative Breast Cancer: A Pairwise and Network Meta-Analysis of Pathological Complete Response
- Author
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Zeng-Jie Weng MM, Sheng-Xi Wu MD, He-San Luo MD, Ze-Sen Du MM, Xu-Yuan Li MD, and Jia-Zhou Lin MM
- Subjects
Public aspects of medicine ,RA1-1270 - Abstract
We performed a pairwise and network meta-analysis to compare pathological complete response (pCR) among neoadjuvant chemotherapy in patients with triple-negative breast cancer. We searched PubMed for randomized clinical trials between January 1, 2000 and December 1, 2020. Abstracts from meetings were also searched. A frequentist random-effect model was applied to compare pCR and toxicities. The P-score was used to rank treatment effects. Nineteen trials with 16 treatments and 7794 patients were included. On the basis of SoC, the addition of carboplatin (OR = 1.82, 95% CI, 1.24 to 2.68, P < .01) and the addition of checkpoint inhibitors (OR = 1.69, 95% CI, 1.23 to 2.32, P < .01) increased pCR in pairwise meta-analysis; compared with paclitaxel, nab-paclitaxel did not improve pCR rates (OR = 1.81, 95% CI, .80 to 4.12, P = .16). The anthracycline-sparing regimen led to similar pCR compared with the anthracycline-containing regimen (OR = 1.50, 95% CI, .82 to 2.76, P = .19). In network meta-analysis, the addition of carboplatin plus a PD-1 inhibitor (pembrolizumab), carboplatin plus bevacizumab, and carboplatin plus veliparib ranked as the top three treatments for achieving pCR, with corresponding P-scores of .91, .84, and .72, respectively. Among patients with homologous recombination deficiency, the addition of carboplatin (OR = 1.31, 95% CI, .69 to 2.50, P = .41) or carboplatin plus PARP inhibitors (OR = 1.19, 95% CI, .58 to 2.47, P = .63) did not increase pCR. For triple-negative breast cancer, combining carboplatin with taxane-anthracycline-containing neoadjuvant chemotherapy could be the standard of care, and the combination containing checkpoint inhibitor is promising. However, their role in long-term oncologic outcome remains to be determined.
- Published
- 2021
- Full Text
- View/download PDF
28. Enzymatic Characteristics of a Recombinant Neutral Protease I (rNpl) from Aspergillus oryzae Expressed in Pichia pastoris.
- Author
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Ye Ke, Wei-Qian Huang, Jia-zhou Li, Ming-quan Xie, and Xiao-chun Luo
- Published
- 2012
- Full Text
- View/download PDF
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