24 results on '"Jian-Guang Qi"'
Search Results
2. Gut microbiota analysis and its significance in vasovagal syncope in children
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Wei Bai, Selena Chen, Chao-Shu Tang, Jian-Guang Qi, Qing-Hua Cui, Ming Xu, Jun-Bao Du, Hong-Fang Jin, and Qiang Shi
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Medicine - Abstract
Abstract. Background:. Vasovagal syncope (VVS) is common in children and greatly affect both physical and mental health. But the mechanisms have not been completely explained. This study was designed to analyze the gut microbiota in children with VVS and explore its clinical significance. Methods:. Fecal samples from 20 VVS children and 20 matched controls were collected, and the microbiota were analyzed by 16S rRNA gene sequencing. The diversity and microbiota compositions of the VVS cases and controls were compared with the independent sample t test or Mann-Whitney U test. The correlation between the predominant bacteria and clinical symptoms was analyzed using Pearson or Spearman correlation test. Results:. No significant differences in diversity were evident between VVS and controls (P > 0.05). At the family level, the relative abundance of Ruminococcaceae was significantly higher in VVS children than in controls (median [Q1, Q3]: 22.10% [16.89%, 27.36%] vs. 13.92% [10.31%, 20.18%], Z = −2.40, P 4, P
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- 2019
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3. Vaccination in children with congenital heart disease: an observational study in a Beijing hospital
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Xue-ying Zhou, Mi Yao, Jian-guang Qi, Zhen-nan Qi, and Wei-lan Liang
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Pediatrics, Perinatology and Child Health - Abstract
Introduction Underimmunization of CHD children is a public health concern in China. This study aimed to analyze the vaccination status of CHD children to provide additional evidence on optimal vaccination strategies and to make suggestions to promote appropriate vaccination services for these children. Methods This cross-sectional study evaluated 155 CHD children who received at least one vaccine at Peking University First Hospital. Vaccine-specific immunization rates were calculated. A telephone questionnaire survey was conducted that covered the following: the prognosis, reasons for delayed vaccinations and getting vaccination in the hospital. All statistical analyses were performed using the SPSS version 22 software. Results The left-to-right shunt group involved 138 children, while the other type CHD group involved 17. The vaccination rate was the highest for MPSV-AC (87.1%) and the lowest for DTaP (40.1%). The most frequent reason for vaccination in the hospital was refusal from community health centers (61.5%). No participant reported vaccine-related adverse effects. Conclusions The age-appropriate vaccine-specific immunization rates in CHD children are low, with the lowest for DTaP. Refusal of community health centers was the primary reason. Our findings support that clinically stable CHD children may be safely vaccinated on a schedule similar to that of ordinary children in China. Impact From our investigation, we found that the age-appropriate vaccine-specific immunization rates in children with CHD in China are low, with the lowest for diphtheria and tetanus toxoid and acellular pertussis. Refusal of community health centers to vaccinate was the primary reason for the low rates. We believe our study provides additional evidence on optimal vaccination strategies for children with CHD and it can be used to develop strategies to promote appropriate vaccination services for these children.
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- 2022
4. The configuration exchanging theory for transport properties and glass formation temperature of ionic liquids.
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Yu-Feng Hu, Xian-Ming Zhang, Jian-Guang Qi, and Liu-Yi Yin
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GLASS transition temperature ,IONIC liquids ,DIFFUSION coefficients ,MOLECULAR rotation ,MOLECULAR structure - Abstract
Understanding molecular motion in terms of molecular structure is an important issue for microscopic understanding of the nature of transport properties and glass transition, and for design of structured materials to meet specific demands in various applications. Herein, a novel molecular mechanism is proposed to connect macroscopic motion in ionic liquids with molecular structure via conformational conversions of the constituent ions or of the cation-anion pairs. New equations for description of relaxation time, diffusion coefficient, molar conductivity, and viscosity of ionic liquids are established. The equation parameters, which were determined from the temperature dependent heat capacities, self-diffusion coefficients, molar conductivities, and viscosities of typical ionic liquids, were used to produce predictions for the corresponding properties of other ionic liquids and for the glass transition temperatures of representative ionic liquids. All predictions are in nice agreements with the experimental results. [ABSTRACT FROM AUTHOR]
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- 2015
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5. Investigation on co-hydropyrolysis of heavy bio-oil with degradable plastics
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Rong-Yang Zhao, Mei-Ling Yang, Jian-guang Qin, Wei-Wei Cui, Lai-Shun Yang, Guo-Zhang Chang, Guang-Xi Yue, and Cui-Ping Wang
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Heavy bio-oil ,Degradable plastics ,Co-hydropyrolysis ,Iron-based oxygen carrier ,Fuel oil ,Environmental technology. Sanitary engineering ,TD1-1066 - Abstract
Reducing the acidity and viscosity of heavy bio-oil is one of the main tasks of bio-oil treatment. The sharp increase in the consumption of degradable plastics requires an effective route for their recycling. In this study, heavy bio-oil was used as the organic solvent of degradable plastics in a co-hydropyrolysis process with lower temperature in reaction kettle. Employing an orthogonal experimental design, and with the maximum total energy yield of co-hydropyrolysis process as the target, the optimal reaction parameters were determined by the degradable plastics all pyrolyzed. The liquidity of the liquid phase increased significantly and the viscosity is decreased. The hydrocarbon content in the liquid-phase products increased, and the amount of organic acids decreased significantly from 183.9 mg/g to 72.1 mg/g according to KOH solution titration. A total energy yield of 86.27% was achieved with a calorific value of the bio-oil product of 25.6 MJ/kg and coke product of 26.93 MJ/kg. The active component of the iron-based oxygen carrier is completely transformed from FeO to Fe3O4, which prompted the pyrolysis of oxygen-containing organic matter and the uptake of the products by the fuel oil. This work developed a way to resource the heavy bio-oil and degradable plastics.
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- 2023
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6. [Clinical analysis and follow-up study of cardiavascular system involvement in 10 children with methylmalonic aciduria combined with hyperhomocysteinemia]
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Yan-Hua, Qi, Jian-Guang, Qi, Yu-Peng, Liu, Hui, Yan, Xue-Qin, Liu, Xin, Zhang, Hui-Jie, Xiao, Yan-Ling, Yang, and Jun-Bao, DU
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Male ,Cardiovascular Diseases ,Child, Preschool ,Hyperhomocysteinemia ,Infant, Newborn ,Humans ,Infant ,Female ,Child ,Amino Acid Metabolism, Inborn Errors ,Follow-Up Studies ,Retrospective Studies - Abstract
To study the clinical features and treatment outcomes of cardiovascular system involvement in children with methylmalonic aciduria combined with hyperhomocysteinemia (MMACHC).The clinical data of 10 children with methylmalonic aciduria combined with hyperhomocysteinemia and who had cardiovascular system involvement were retrospectively analyzed and the treatment outcomes were followed up.In the 10 patients, there were 4 cases with initial presentations of cardiovascular system symptoms such as shortness of breath and dyspnea, 3 cases with urinary tract symptoms such as edema, hematuria and proteinuria, and 3 cases with nervous system symptoms such as developmental retardation and convulsions. The 10 patients had different types and severity of cardiovascular injuries. After 3 months to 8 years of follow-up, the congenital heart defects resolved naturally in 2 cases, and the patient with arrhythmia had no obvious changes. In 5 cases of hypertension, blood pressures recovered to normal in 3 cases, and 1 case was lost to follow-up. In 5 patients with pulmonary hypertension, 2 died, 2 recovered, and 1 case had mildly elevated pulmonary artery pressure. Seven patients underwent MMACHC gene testing, and 5 showed c.80AG mutations.Metabolic disease should be taken into account for the children with unexplained pulmonary hypertension and hypertension with the onset of the shortness of breath and dyspnea. The severity of cardiovascular system involvement might be one of the most important factors affecting the prognosis of children with MMACHC. Cardiavascular system involvement of the patients may be related to MMACHC c.80AG mutations.
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- 2015
7. [Clinical characteristics and follow-up study of tachycardia-induced cardiomyopathy in 12 children]
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Jian-guang, Qi, Chang-qing, Xing, Xue-qin, Liu, Qing-you, Zhang, Yong-hong, Chen, and Jun-bao, DU
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Cardiomyopathy, Dilated ,Male ,Child, Preschool ,Tachycardia ,Humans ,Infant ,Female ,Cardiomyopathies ,Child ,Follow-Up Studies ,Retrospective Studies - Abstract
Tachycardia induced cardiomyopathy (TIC), secondary to various tachyarrhythmias, is a reversible condition which can lead to cardiac enlargement and heart failure. The impairment of both structure and function of heart can be reverted completely or partially if tachyarrhythmias are ceased without delay. This study aimed to explore the clinical characteristics, therapeutic regimen and outcome of TIC in children.Clinical data of 12 children with TIC, who came from Peking University First Hospital from Feb. 2003 to Jun. 2009, were retrospectively analyzed and followed up. The echocardiogram data on admission were compared with those from 12 homochronous cases with idiopathic dilated cardiomyopathy matched with 12 TIC cases in age and gender.Atrial tachycardia is the commonest arrhythmia in 12 TIC cases (75%). Four cases underwent catheterization for radiofrequency ablation and all succeeded. The cardiac rhythm of 6 out of 8 cases treated with drugs became sinus rhythm after 3 days to 2 weeks antiarrhythmic drugs treatment. The remaining 2 cases still retained atrial rhythm, but the ventricular heart rates declined to normal. The left ventricular end-diastolic dimensions of the 12 cases were decreased compared with those of pretherapy [(37.5 ± 5.3) mm vs. (43.0 ± 5.7) mm, P0.01], and the left ventricular ejection fractions were increased [(60.5% ± 5.6%) vs. (33.7% ± 10.3%), P0.01], after (3.4 ± 2.3) months. In our (4.3 ± 2.4) year-follow-up, all cases were fine, except in one case the tachyarrhythmia relapsed because of discontinuation of the drug treatment by her parents. The left ventricular end-diastolic dimensions in 12 TIC cases were smaller than those of the 12 age- and gender-matched idiopathic dilated cardiomyopathy [(43.0 ± 5.7) mm vs. (54.8 ± 7.5) mm, t = 7.9, P0.01], and the ejection fractions were higher [(33.7% ± 10.3%) vs. (21.8% ± 7.5%), t = 3.7, P0.01].The diagnosis of TIC should be considered for the children with tachycardia, cardiac enlargement and cardiac insufficiency. The degree of cardiac enlargement and cardiac insufficiency might be of value for the differential diagnosis between TIC and idiopathic dilated cardiomyopathy. The rhythm control and ventricular rates control could all result in a favorite therapeutic efficacy.
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- 2012
8. [Adrenomedullin alleviates collagen accumulation in pulmonary arteries of rats with hypoxic pulmonary hypertension]
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Jian-Guang, Qi, Chang-Qing, Xing, Ya-Guang, Ding, and Jun-Bao, DU
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Male ,Adrenomedullin ,Transforming Growth Factor beta ,Hypertension, Pulmonary ,Animals ,Collagen ,Pulmonary Artery ,Rats, Wistar ,Hypoxia ,Rats - Abstract
To observe the effect of adrenomedullin (ADM) on the pulmonary vascular collagen metabolism in hypoxic rats in order to study the effect of ADM on chronic hypoxic pulmonary vascular structural remodeling and its possible mechanism.Nineteen male Wistar rats were randomly divided into three groups: normal control (n=6), hypoxia (n=7) and ADM-treated hypoxia (n=6). ADM was subcutaneously administered into rats of the ADM-treated hypoxia group by mini-osmotic pump (300 ng/h) for two weeks. After two weeks of hypoxic challenge, mean pulmonary arterial pressure (mPAP) was evaluated using a right cardiac catheterization procedure. The ratio of right ventricular mass to left ventricular plus septal mass[RV/ (LV+S)] was measured. The changes of pulmonary vascular microstructure were observed. Meanwhile, the expression levels of collagen I, collagen III and transforming growth factor (TGF)-β in pulmonary arteries were detected by immunohistochemical assay.mPAP and RV/(LV+S) increased significantly in the hypoxia group compared with normal controls (P0.01). The muscularization of small pulmonary vessels and the relative medial thickness of pulmonary arteries increased obviously in the hypoxia group compared with those in the normal control group (P0.01). Meanwhile, the expression levels of collagen I, collagen III and TGF-β of pulmonary arteries in the hypoxia group increased markedly compared with those in the normal control group. However, mPAP and RV/(LV+S) were significantly reduced in the ADM-treated hypoxia group compared with those in the hypoxia group (P0.01). ADM ameliorated pulmonary vascular structural remodeling of hypoxic rats, with a decrease in the expression of collagen I, collagen III and TGF-β of pulmonary arteries.ADM might play a regulatory role in the development of hypoxic pulmonary hypertension and hypoxic pulmonary vascular remodeling, through inhibiting the expression of TGF-β and alleviating the collagen accumulation of pulmonary arteries.
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- 2012
9. [Impact of high pulmonary blood flow on pulmonary vascular structure and human urotensin II in intrapulmonary arteries of rats]
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Jian-Guang, Qi, Jun-Bao, Du, Jian, Li, Bing, Wei, and Chao-Shu, Tang
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Male ,Rats, Sprague-Dawley ,Pulmonary Circulation ,Hypertension, Pulmonary ,Urotensins ,Animals ,Humans ,Pulmonary Artery ,Rats - Abstract
To study human urotensin II (hUII) expression in intrapulmonary arteries of rats with pulmonary hypertension induced by high pulmonary blood flow and explore the role of hU II in the development of pulmonary hypertension induced by left to right shunt.Aortocaval shunting was produced for 11 weeks in rats. Pulmonary artery mean pressure (PAMP) of each rat was evaluated using right cardiac catheterization. The pulmonary vascular structural changes, including the percentage of muscularized arteries of small pulmonary vessels and relative medial thickness of intra-acinar pulmonary arteries were examined. Meanwhile, the expression of hU II by pulmonary arteries was detected by immunohistochemistry.After 11-week aortocaval shunting, PAMP was significantly increased. The percentage of muscularized arteries of small pulmonary vessels and relative medial thickness of pulmonary arteries were obviously increased in shunting rats compared with controls (P0.01, respectively). Meanwhile, hU II expression by pulmonary artery endothelial cells and smooth muscle cells was significantly augmented in rats of shunt group, which was positively correlated with PAMP and the structural changes in pulmonary arteries.The up-regulation of hU II in pulmonary arteries might be involved in the development of pulmonary vascular structural remodeling and pulmonary hypertension induced by high pulmonary blood flow.
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- 2010
10. [Genotype, phenotype analysis and follow-up study on patients with Duchenne/Becker muscular dystrophy]
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Yan-zhi, Zhang, Hui, Xiong, Xiao-zhu, Wang, Shuo, Wang, Jing, Luo, Jing-min, Wang, Yu-wu, Jiang, Xing-zhi, Chang, Hong, Pan, Jian-guang, Qi, Wan-zhen, Li, Yun, Yuan, and Xi-ru, Wu
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Male ,Adolescent ,Genotype ,DNA Mutational Analysis ,Infant ,Exons ,Muscular Dystrophy, Duchenne ,Young Adult ,Phenotype ,Child, Preschool ,Mutation ,Humans ,Female ,Child ,Nucleic Acid Amplification Techniques ,Gene Deletion ,Follow-Up Studies - Abstract
To improve the diagnosis and management of Duchenne/Becker muscular dystrophy(DMD/BMD).Clinical features of 90 cases of DMD/BMD were collected. Genomic DNA was extracted using standard procedures from the peripheral blood leukocytes, and multiplex ligation-dependent probe amplification (MLPA) was applied to detect DMD gene to identify genetic mutation. For those patients whose deletion/duplication mutation was not identified, FKRP gene mutation analysis was performed using PCR-DNA direct sequence. All the cases were followed up.Among the 90 cases of clinically diagnosed DMD/BMD, exons deletion of DMD was detected in 58 cases (64.44%), and exons duplication in 9 (10.00%). Among the 34 mothers with an affected boy but without previous genetic conformation, 17 were confirmed to be carriers with gene deletion/duplication. None of the 23 cases, without detected DMD gene deletion/duplication, carried FKRP gene mutation. Fourteen children were given short-term intermittent prednisone therapy (0.75 mg/kg daily during the first 10 days of each month). The course was not long enough and the sample size was too small to conclude any benefits or side effects. Prenatal diagnosis was provided for one mother in her next pregnancy detecting a female carrier fetus.DMD gene deletions mainly occurs between exons 45 and 54, while duplications mostly at 5'-terminus. Identification of the characteristics and types of gene mutation may facilitate the recognition and prognosis prediction of DMD/BMD. MLPA is a non-complex and quick diagnostic tool for DMD/BMD and its carriers, and also helpful in genetic counseling.
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- 2010
11. [Regulation of endogenous cystathionine-gamma-lyase gene expression in high pulmonary flow by nitric oxide precursor]
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Lin, Shi, Jun-bao, Du, Ding-fang, Pu, Jian-guang, Qi, and Chao-shu, Tang
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Male ,Rats, Sprague-Dawley ,Gene Expression Regulation ,Cystathionine gamma-Lyase ,Animals ,Gene Expression ,Hydrogen Sulfide ,Arginine ,Nitric Oxide ,Lung ,Rats - Abstract
Pulmonary hypertension is a common complication of congenital heart disease with a left-to right shunt. The mechanism of pulmonary hypertension induced by high pulmonary blood flow is still not fully understood. Recent studies showed that hydrogen sulfide (H2S) could relax vascular smooth muscle cells. But the change of the system of H2S in pulmonary hypertension induced by high pulmonary blood flow was not reported. We studied the influence on expression of CSE mRNA and production of hydrogen sulfide in rat lung tissues by L-Arginine, in order to demonstrate a regulating role of nitric oxide (NO) in the regulation of cystathionine-gamma-lyase/hydrogen sulfide system (CSE/H2S).Thirty male SD rats were randomly divided into shunting group, shunting with L-Arginine group, and control group. Abdominal aorta and inferior vena cava shunting was produced in rats of the later group. Pulmonary artery mean pressure (mPAP) and the hypertrophy of right ventricle of each rat were analyzed. The expression of lung tissue CSE mRNA was measured using quantitative reverse transcription-polymerase chain reaction and in situ hybridization. The activity of CSE in lung tissue was measured according to chemical analysis.mPAP was significantly increased in shunted rats compared with normal control (P0.01), the expression of lung tissue CSE mRNA and the activity of CSE in lung tissue were decreased in shunt group (P0.01). However, L-arginine significantly attenuated pulmonary artery pressure, but augmented the expression of lung tissue CSE mRNA as well as the activity of CSE in lung tissue.L-Arginine reverses the down-regulation of CSE/H2S system in high pulmonary blood flow-induced pulmonary hypertension.
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- 2010
12. [Clinical characteristics of cardiac syncope in children]
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Qing-you, Zhang, Jun-bao, DU, Jian-guang, Qi, Ling, Han, and Wan-zhen, Li
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Diagnosis, Differential ,Male ,Adolescent ,Heart Diseases ,Child, Preschool ,Tachycardia, Ventricular ,Humans ,Female ,Child ,Syncope ,Retrospective Studies - Abstract
To explore the clinical characteristics of cardiac syncope (CS) in children, and understand their significance in predicting the cardiac syncope.Twenty-three patients were referred to our department for evaluation of syncope. The diagnosis of the above cases was cardiac syncope. Each patient was interviewed using a standard questionnaire. The clinical histories and standard baseline electrocardiogram were analyzed to identify the variables contributing to the diagnosis of CS in children.A cardiac cause was identified in 23 syncopal patients presenting to the Department of Pediatrics, Peking University First Hospital: sick sinus syndrome in 7, congenital long QT syndrome in 4, third degree atrioventricular block in 2, supraventricular tachycardia in 2, ventricular tachycardia in 1, atrial fibrillation in 1, pacemaker dysfunction in 1, idiopathic pulmonary hypertension in 3, hypertrophic cardiomyopathy in 1, and dilated cardiomyopathy in 1. The average age of CS patients was 9 years. In totally 23 patients, exertion related syncope spells were found in 14 cases (60.9%), syncope spells at various position 7/23 (30.4%), absence of prodromes in 12/23 (52.2%), syncope spells with incontinence in 4/23 (17.4%), history of heart disease in 4/23 (17.4%). Abnormal standard baseline electrocardiogram was found in 21 cases (91.7%).The children with cardiac syncope have overt clinical features, especially abnormal findings in electrocardiogram and exertion related syncope spells are the most common clinical features.
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- 2009
13. Suppression of CCDC6 sensitizes tumor to oncolytic virus M1
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Ying Liu, Ke Li, Wen-bo Zhu, Hao Zhang, Wen-tao Huang, Xin-cheng Liu, Yuan Lin, Jing Cai, Guang-mei Yan, Jian-guang Qiu, Liang Peng, Jian-kai Liang, and Cheng Hu
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Oncolytic virus ,M1 virus ,CCDC6 ,Interferon pathway ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Oncolytic virus is an effective therapeutic strategy for cancer treatment, which exploits natural or manipulated viruses to selectively target and kill cancer cells. However, the innate antiviral system of cancer cells may resistant to the treatment of oncolytic virus. M1 virus is a newly identified oncolytic virus belonging to alphavirus species, but the molecular mechanisms underlying its anticancer activity are largely unknown. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. RNA seq analysis was used to analyze the gene alternation after M1 virus infection. Small interfering RNAs transfection for gene knockdown was used for gene functional tests. Caspase-3/7 activity was detected by Caspase-Glo Assay Systems. A mice model of orthotopic bladder tumor was established to determine the oncolytic effectiveness of the M1 virus. The expression of cleaved-Caspase 3 as well as Ki-67 in tumor cells were detected by immunohistochemical analysis. To further define the molecular factors involved in M1 virus-mediated biological function, we knocked down genes related to alphavirus’ activity and found that CCDC6 plays an important role in the oncolytic activity of M1 virus. Moreover, knocked down of CCDC6 augments the reproduction of M1 virus and resulted in endoplasmic reticulum (ER) stress-induced cell apoptosis in vitro as well as in vivo orthotopic bladder cancer model. Our research provides a rational new target for developing new compounds to promote the efficacy of oncolytic virus therapy.
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- 2021
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14. [Clinical characteristics and therapy of severe Mycoplasma pneumonia in children]
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Jian-Guang, Qi, Shao-Jie, Zhang, and Yong-Hong, Chen
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Male ,Leukocyte Count ,C-Reactive Protein ,Adolescent ,Child, Preschool ,Pneumonia, Mycoplasma ,Humans ,Female ,Blood Sedimentation ,Child ,Antibodies, Bacterial - Abstract
To study the clinical characteristics, therapeutic regimen and outcome of severe Mycoplasma pneumonia (MP) in children.Clinical data of 79 children with MP, including 69 mild and 10 severe cases, were retrospectively analyzed. The 10 children with severe MP were followed-up.In severe MP cases, the fever duration prior to hospitalization and the total fever duration were more prolonged, peripheral blood leucocytes counts, C-reactive protein and erythrocyte sedimentation rate increased, and serum IgM and IgE levels increased as compared to mild MP cases. Of the 10 cases of severe MP, 4 manifested as pulmonary consolidation, 4 as pulmonary consolidation complicated by moderate to large pleural effusion and 2 as progressively worsening pulmonary radiographic findings. Nine severe MP cases were administered with glucocorticoid as well as antibiotics, and the therapeutic effect was satisfactory. In the convalescence stage, bronchofiberoscope lavages were used in 5 severe cases because of persistent pulmonary consolidation.Severe MP was characterized by rapid progression, pulmonary consolidation, moderate to severe pleural effusion, obviously increased inflammatory indexes, and poor therapeutic reaction to simple macrolide antibiotics. Besides antibiotics, glucocorticoid should be used for severe MP cases as soon as possible. For severe cases with persistent pulmonary consolidation, bronchofiberoscope lavages are recommended.
- Published
- 2008
15. [Alterations of proadrenomedullin N-terminal 20-peptide in rats with pulmonary hypertension induced by high pulmonary blood flow]
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Jian-Guang, Qi, Xiao-Hui, Li, Ya-Guang, Ding, Chao-Shu, Tang, and Jun-Bao, Du
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Male ,Rats, Sprague-Dawley ,Adrenomedullin ,Pulmonary Circulation ,Hypertension, Pulmonary ,Animals ,Pulmonary Artery ,Rats - Abstract
The mechanism of high pulmonary blood flow-induced pulmonary hypertension remains unclear. The aim of this study was to explore the effect of proadrenomedullin N-terminal 20-peptide (PAMP) on pulmonary hypertension, through examining the alterations of pulmonary PAMP expression and plasma PAMP concentration in rats with pulmonary hypertension induced by high pulmonary blood flow.Sixteen male Sprague-Dawley rats were randomly divided into control (n=8) and shunt groups (n=8). Aortocaval shunting was produced in the shunt group. After 11 weeks of shunting, systolic pulmonary artery pressure (sPAP), diastolic pulmonary artery pressure (dPAP) and mean pulmonary artery pressure (mPAP) were evaluated by using a right cardiac catheterization procedure. The ultrastructural changes in intra-acinar pulmonary arteries were observed. The concentration of plasma PAMP was measured by radioimmunoassay. The expression of PAMP in pulmonary arteries was detected by immunohistochemical assay.sPAP, dPAP and mPAP were significantly increased in shunt rats compared with controls (P0.01). Ultrastructural changes, such as hyperplasia and swelling of endothelial cells, irregularity of internal elastic laminar, and hypertrophy and increased number of synthetic phenotype of smooth muscle cells, were found in intra-acinar pulmonary muscularized arteries in the shunt group. Plasma PAMP concentration (616 +/- 195 pg /mL vs 427 +/- 90 pg /mL) and PAMP expression in endothelial cells (0.62 +/- 0.09 vs 0.38 +/- 0.12) and in smooth muscle cells (0.24 +/- 0.07 vs 0.14 +/- 0.05) of pulmonary arteries increased significantly in the shut group compared with controls.The up-regulation of pulmonary and plasm PAMP expression might be involved in the development of high pulmonary blood flow-induced pulmonary hypertension.
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- 2007
16. Imbalance of endogenous homocysteine and hydrogen sulfide metabolic pathway in essential hypertensive children
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Li, Chen, Sumou, Ingrid, Ya-guang, Ding, Ying, Liu, Jian-guang, Qi, Chao-shu, Tang, and Jun-bao, DU
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Male ,Adolescent ,Systole ,Hypertension ,Humans ,Female ,Hydrogen Sulfide ,Child ,Homocysteine - Abstract
Hypertension is a common disease of the cardiovascular system. So far, the pathogenesis of primary hypertension remains unclear. The elaboration of its pathogenesis is an important topic in the field which calls for urgent resolution. The aim of this study was to probe into the metabolic imbalance of homocysteine (Hcy) and hydrogen sulfide (H(2)S) in children with essential hypertension, and its significance in the pathogenesis of essential hypertension.Twenty-five children with essential hypertension and 30 healthy children with normal blood pressure were enrolled in the study. The medical history was investigated and a physical examination was conducted on the subjects. Plasma Hcy content was examined by fluorescence polarization immunoassay (FPIA). The plasma H(2)S level was detected by a modified method with a sulfide electrode. Data were presented as mean +/- standard deviation. The t test was applied to the mean values of both groups. Pearson linear correlation analysis was applied to the plasma Hcy and H(2)S as well as to the systolic pressure against the plasma H(2)S/Hcy ratio.Plasma Hcy, an intermittent metabolite of the endogenous methionine pathway, was markedly increased but plasma H(2)S, a final product of this pathway was significantly decreased in hypertensive cases when compared with normal subjects ((Hcy: (12.68 +/- 9.69) micromol/L vs (6.62 +/- 4.79) micromol/L (t = 2.996, P0.01); H(2)S: (51.93 +/- 6.01) micromol/L vs (65.70 +/- 5.50) micromol/L) (t = -8.670, P0.01)). The ratio of plasma H(2)S/Hcy in children with hypertension was 5.83 +/- 2.91, while that of the control group was 11.60 +/- 3.30, and the difference is significant with a t = -6.610 and P0.01. A negative correlation existed between plasma Hcy and H(2)S concentrations, r = -0.379, P0.05. And a negative correlation was found between systolic blood pressure and the plasma H(2)S/Hcy ratio, r = -0.687, P0.05.There was a metabolic imbalance of homocysteine and hydrogen sulfide in essential hypertensive children.
- Published
- 2007
17. [Effect of adrenomedullin 1-50 on chronic hypoxic pulmonary hypertension in rats]
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Jian-guang, Qi, Ya-guang, Ding, Chao-shu, Tang, and Jun-bao, Du
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Male ,Hypertension, Pulmonary ,Pulmonary Artery ,Nitric Oxide ,Peptide Fragments ,Rats ,Adrenomedullin ,Chronic Disease ,Airway Remodeling ,Animals ,Hydrogen Sulfide ,Rats, Wistar ,Hypoxia ,Lung - Abstract
To explore the effect of adrenomedullin(1-50) (ADM(1-50)) on hypoxic pulmonary hypertension and pulmonary vascular structural remodeling and the plasma concentration of nitric oxide (NO) and hydrogen sulfide (H(2)S) in rats.Twenty male Wistar rats were randomly divided into control group (n=7), hypoxic group (n=6) and hypoxic with ADM(1-50) group (n=7). ADM(1-50) was subcutaneously administered into rats of hypoxic with ADM(1-50) group by mini-osmotic pump (300 ng/h). After two weeks' hypoxic challenge, mean pulmonary arterial pressure (mPAP) was evaluated by using a right cardiac catheterization procedure. The ratio of right ventricular mass to left ventricular plus septal mass [RV/(LV+S)] was detected. Pulmonary vascular microstructure was measured and the ultrastructural changes in intra-acinar pulmonary arteries were observed. Meanwhile, plasma concentrations of NO and H(2)S were measured.mPAP was significantly increased in hypoxic rats than that in controls [(24.9+/-6.8) mmHg vs (14.3+/-2.4) mmHg, P0.01,1 mmHg=0.133 kPa]; RV/(LV+S) was also significantly increased in hypoxic rats than that in controls [(0.318+/-0.054) vs (0.182+/-0.007), P0.01]. Microstructure and ultrastructure of pulmonary arteries changed obviously in hypoxic rats with the development of hypoxic pulmonary vascular structural remodeling. Meanwhile, plasma NO and H(2)S concentrations in hypoxic rats were markedly decreased compared with controls. However, mPAP was significantly decreased in hypoxic rats treated with ADM(1-50) than that in hypoxic rats [(14.9+/-3.0) mmHg vs (24.9+/-6.8) mmHg, P0.01]; RV/(LV+S) was also significantly decreased than that in hypoxic rats [(0.185+/-0.011) vs (0.318+/-0.054), P0.01]. ADM(1-50) ameliorated pulmonary vascular structural remodeling of hypoxic rats in association with an increase in plasma NO and H(2)S concentrations.ADM(1-50) plays an important role in regulation of the development of hypoxic pulmonary hypertension and hypoxic pulmonary vascular structural remodeling, through promoting NO and H(2)S production in hypoxic rats.
- Published
- 2006
18. [A case of Kawasaki disease with anterior uveitis]
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Jian-guang, Qi, Jun-bao, Du, Yong-hong, Chen, Yun, Qi, and Wan-zhen, Li
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Adolescent ,Humans ,Female ,Mucocutaneous Lymph Node Syndrome ,Uveitis, Anterior - Published
- 2005
19. [Endogenous nitric oxide pathway in high pulmonary blood flow-induced pulmonary vascular structural remodeling]
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Jian-guang, Qi, Jun-bao, Du, Xiu-ying, Tang, Jian, Li, Bing, Wei, and Chao-shu, Tang
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Male ,Rats, Sprague-Dawley ,Nitric Oxide Synthase Type III ,Hypertension, Pulmonary ,Animals ,RNA, Messenger ,Nitric Oxide Synthase ,Pulmonary Artery ,Nitric Oxide ,Immunohistochemistry ,Blood Flow Velocity ,In Situ Hybridization ,Rats - Abstract
Pulmonary vascular structural remodeling induced by high pulmonary blood flow is an important pathologic basis of pulmonary hypertension with congenital heart disease of left-to-right shunt. However, the mechanism is still not clear. The present study aimed to examine the alteration of endogenous nitric oxide (NO) pathway in high pulmonary blood flow-induced pulmonary vascular structural remodeling, so as to explore the role of NO pathway in pulmonary hypertension induced by high pulmonary blood flow.Sixteen male SD rats were randomly divided into control group (n = 8) and shunting group (n = 8). Aortocaval shunting was produced for 11 weeks in shunt rats. Pulmonary artery mean pressure (mPAP) of each rat was evaluated using right cardiac catheterization. The ratio of right ventricular mass to left ventricular plus septal mass [RV/(LV + S)] was detected. Pulmonary vascular micro-and ultra-structure was examined by using a light microscope and a transmitted electronic microscope. Meanwhile, the concentration of plasma NO was measured by spectrophotometry. The expressions of endothelial NO synthase (eNOS) mRNA and protein by pulmonary arteries were detected by in situ hybridization and immunohistochemistry, respectively.After 11-week aortocaval shunting, mPAP was significantly increased [(22.5 +/- 2.6) mmHg vs. (15.8 +/- 2.8) mmHg, 1 mmHg = 0.133 kPa, t = 4.97, P0.01], and RV/(LV + S) was also markedly increased (0.267 +/- 0.022 vs. 0.221 +/- 0.016, t = 4.85, P0.01). The percentage of muscularized arteries was obviously increased in shunt rats compared with controls [(23.2 +/- 2.4)% vs. (13.5 +/- 2.1)%, t = 7.82, P0.01], and relative medial thickness of pulmonary arteries was obviously increased in shunt rats [median pulmonary artery: (7.76 +/- 0.56)% vs. (4.82 +/- 1.03)%, t = 6.23, P0.01; small pulmonary artery: (11.94 +/- 0.66)% vs. (6.91 +/- 0.53)%, t = 14.96, P0.01]. Ultrastructural changes, such as hyperplasia and degeneration of endothelial cells, irregularity of internal elastic laminar and hypertrophy and the increased number of synthetic phenotype of smooth muscle cells, were found in intrapulmonary arteries of shunt rats. Meanwhile, plasma NO concentration was increased [(30.2 +/- 7.9) micromol/L vs (19.7 +/- 5.7) micromol/L, t = 3.05, P0.01) and eNOS mRNA and protein expressions by pulmonary arteries were significantly augmented in rats of shunting group.The upregulation of eNOS/NO might be an adaptive response of pulmonary circulation to an increased blood flow in the development of pulmonary hypertension and pulmonary vascular structural remodeling.
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- 2004
20. Density and Viscosity of the Ternary Systems [NMP][MSA] + LaCl3 + H2O, [NMP][BSA] + LaCl3 + H2O, and [NMP][p-TSA] + LaCl3 + H2O at Different Temperatures and Atmospheric Pressure.
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Wei-Ting Ma, Yu-Feng Hu, Jian-Guang Qi, Xian-Ming Zhang, and Ya-Mei Zhao
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- 2016
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21. Acyl-CoA synthetase long-chain 3 regulates AKT phosphorylation and the functional activity of human prostate cancer cells
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Ke Li, Yun-Hua Mao, Wen-Han Qiu, Jian-Wen He, De-Juan Wang, Cheng Hu, Wen-Tao Huang, Jie Si-Tu, and Jian-Guang Qiu
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Medicine ,Biology (General) ,QH301-705.5 - Abstract
Abstract. Metastasis is the main cause of cancer-specific death in patients with prostate cancer (PCa). Acyl-coenzyme A synthetase long-chain family member 3 (ACSL3) is involved in the metabolic reprogramming of multiple types of cancer cells, but its role in PCa metastasis remains largely unknown. Here, we determined the effect of overexpression or small interfering RNA-mediated depletion of ACSL3 on the migratory and invasive abilities of human PCa cell lines. We also conducted phospho-protein microarray analysis to identify signaling pathway components affected by ACSL3 modulation. Overexpression of ACSL3 promoted the migration and invasion of PCa cells, whereas ACSL3 downregulation had the opposite effects. Mechanistically, phospho-protein analysis showed that ACSL3 regulated the phosphorylation of AKT and the expression of matrix metalloproteinase9. Our results support a potential role for ACSL3 in promoting the metastatic behavior of PCa, possibly via AKT/matrix metalloproteinase9 pathways. Thus, ACSL3 could be a novel target for the development of treatments for PCa.
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- 2018
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22. Effect of L-Arginine on Pulmonary Artery Smooth Muscle Cell Apoptosis in Rats with Hypoxic Pulmonary Vascular Structural Remodeling.
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Sumou, Ingrid Karmane, Jun-Bao Du, Bing Wei, Chun-Yu Zhang, Jian-Guang Qi, and Chao-Shu Tang
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ARGININE ,AMINO acids ,APOPTOSIS ,PULMONARY artery ,ARTERIES ,PULMONARY blood vessels ,SMOOTH muscle ,HYPOXEMIA - Abstract
This study investigated the effect of L-arginine (L-Arg) on the apoptosis of pulmonary artery smooth muscle cells (PASMC) in rats with hypoxic pulmonary vascular structural remodeling, and its mechanisms. Seventeen Wistar rats were randomly divided into a control group ( n= 5), a hypoxia group ( n= 7), and a hypoxia+L-Arg group ( n= 5). The morphologic changes of lung tissues were observed under optical microscope. Using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphatebiotin nick end labeling assay, the apoptosis of PASMC was examined. Fas expression in PASMC was examined using immunohistochemistry. The results showed that the percentage of muscularized artery in small pulmonary vessels, and the relative medial thickness and relative medial area of the small and median pulmonary muscularized arteries in the hypoxic group were all significantly increased. Pulmonary vascular structural remodeling developed after hypoxia. Apoptotic smooth muscle cells of the small and median pulmonary arteries in the hypoxia group were significantly less than those in the control group. After 14 d of hypoxia, Fas expression by smooth muscle cells of median and small pulmonary arteries was significantly inhibited. L-Arg significantly inhibited hypoxic pulmonary vascular structural remodeling in association with an augmentation of apoptosis of smooth muscle cells as well as Fas expression in PASMC. These results showed that L-Arg could play an important role in attenuating hypoxic pulmonary vascular structural remodeling by upregulating Fas expression in PASMC, thus promoting the apoptosis of PASMC. Edited by Huang-Tian YANG [ABSTRACT FROM AUTHOR]
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- 2006
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23. Comparative study on ureteroscopic lithotripsy and laparoscopic ureterolithotomy for treatment of unilateral upper ureteral stones Estudo comparativo entre litotripsia e ureterolitotomia laparoscópica no tratamento de cálculos unilaterais altos
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You-qiang Fang, Jian-guang Qiu, De-juan Wang, Hai-lun Zhan, and Jie Situ
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Cálculos Ureterais ,Ureteroscopia ,Litotripsia ,Laparoscopia ,Ureteral Calculi ,Ureteroscopy ,Lithotripsy ,Laparoscopy ,Surgery ,RD1-811 - Abstract
PURPOSE: To compare the curative effects of ureteroscopic lithotripsy and laparoscopic ureterolithotomy for unilateral upper ureteral stones, and to explore optimal surgical indications and skills. METHODS: Fifty cases of unilateral upper ureteral stones were randomly divided into two groups: one group underwent ureteroscopic holmium laser lithotripsy under epidural or lumbar anesthesia (n=25), and another group underwent laparoscopic ureterolithotomy under general anesthesia (n=25). Double-J stent was routinely indwelled in both groups. Operating time, postoperative hospitalization time, stone clearance rate and perioperative complications were compared. RESULTS: Operation was successfully performed in all 50 cases, and no open surgery was converted in any case. In the ureteroscopy and laparoscopy groups, the mean operating time was 49.0±10.7 min and 41.8±8.0 min (t=2.68, P=0.00999), respectively, their hospitalization time was 2.8±1.3 days vs. 2.9±0.8 days (t =-0.40, P=0.69413), and stone clearance rate was 88.0% (22/25) vs. 100% (25/25). Stone moved to the renal pelvis in three cases in the ureteroscopy group, and residual stones were removed by extracorporeal shock-wave lithotripsy (ESWL). All patients were followed up for more than three months, and no serious complications such as ureterostenosis occurred. CONCLUSIONS: Laparoscopic ureterolithotomy has a higher stone clearance rate and shorter operation time compared with ureteroscopic lithotripsy. Laparoscopic ureterolithotomy is one safe and effective treatment on unilateral upper ureteral stones.OBJETIVO: Comparar os efeitos curativos da litotripsia ureteroscópica e a ureterolitotomia laparoscópica para cálculos unilaterais altos e pesquisar as indicações e resultados. MÉTODOS: Cinquenta casos de cálculos unilaterais altos foram distribuídos aleatoriamente em dois grupos: um grupo submetido a litotripsia ureteroscópica com laser holmium sob anestesia epidural ou lombar (n=25) e outro grupo submetido a ureterolitotomia laparoscópica sob anestesia geral (n=25). Duplo-J stent foi rotineiramente instalado em ambos os grupos. Comparou-se o tempo operatório, tempo de hospitalização pós-operatória, nível de desaparecimento dos cálculos e complicações pós-operatórias. RESULTADOS: Atos operatórios nos 50 casos sem ocorrências e nenhum ato convertido. Nos grupos por ureteroscopia e laparoscopia, o tempo operatório médio foi 49,0±10,7 minutos e 41,8±8,0 minutos (t=2,68, P=0,00999) respectivamente, tempo de hospitalização foi 2,8±1,3 dias vs. 2,9±0,8 dias (t=0,40, P=0,69413) e o nível de desaparecimento dos cálculos foi 88.0% (22/25) vs. 100% (25/25). Cálculo deslocado para pelve renal em três casos no grupo ureteroscópico e cálculos residuais foram removidos por litotripsia por onda de choque extracorpóreo (ESWL). Todos pacientes foram seguidos por mais de três meses e não ocorreram complicações sérias como estenoses ureterais. CONCLUSÕES: A ureterolitotomia laparoscópica teve maior nível desaparecimento dos cálculos e tempo operatório menor comparado à litotripsia ureteroscópica A ureterolitotomia laparoscópica é um tratamento seguro e efetivo para cálculos ureterais unilaterais altos.
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- 2012
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24. High Q-factor multiple Fano resonances for high-sensitivity sensing in all-dielectric nanocylinder dimer metamaterials.
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Wu-Deng Wang, Li Zheng, and Jian-Guang Qi
- Abstract
We investigated the high Q-factor, high modulation depth, and multiple Fano resonances based on a periodical all-dielectric asymmetric nanocylinder dimer. It is demonstrated that due to excitation of subradiant hybrid modes five sharp Fano resonances can be achieved by breaking symmetry and can be flexibly tuned by geometry. All Fano resonances have a narrow line width, maximal Q-factor exceeds 5000 and even minimal Q-factor also reaches 870. Moreover, modulation depth can reach nearly 100%. In addition, the bulk refractive index sensitivity exceeds 530 nm/RIU, and maximal figure of merit reaches 3350. The proposed array is useful for nonlinear optics and biosensing. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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