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In this paper, the method of reducing the rise time of high-voltage pulse transformer output rectangular pulses using a magnetic compression switch has been studied. An equivalent circuit consisting of pulse transformer and magnetic switch was used to describe the behavior of a transformer operating with magnetic switch. Meanwhile, four simplified equivalent sub-circuits and corresponding differential calculation models were used to analysis the influence of the magnetic switch module on the waveform characteristics of load resistance. The simulation and experiment results showed that the rise time of rectangular pulses could be reduced from more than 1 μs to less than 150 ns while keeping pulse duration constant which was considered as the principle of rectangular pulse sharpening. Moreover, the pulse waveform performance on load resistance was determined by the pulse transformer, magnetic switch module and their coupling effects.

The students' interest in genetic experiments was promoted effectively by adding more genetic projects with research or autonomous selections, adopting the vivid and interesting case and problem-based teaching methods, using the teaching philosophy of diversity to design experimental teaching tools, and taking other comprehensive teaching methods.

Pulsed electric field (PEF) is a novel non-thermal food processing technology that involves the electric discharge of high voltage short pulses through the food product. In PEF study, rectangular pulses are most commonly used for inactivating microorganisms. However, little information is available on the inactivation effect of rising time of rectangular pulse. In this paper, inactivation effects, electric field strength, treatment time and conductivity on staphylococcus aureus inactivation were investigated when the pulse rising time is reduced from 2.5 μs to 200 ns. Experimental results showed that inactivation effect of PEF increased with electric field strength, solution conductivity and treatment time. Rising time of the rectangular pulse had a significant effect on the inactivation of staphylococcus aureus. Rectangular pulses with a rising time of 200 ns had a better inactivation effect than that with 2 μs. In addition, temperature increase of the solution treated by pulses with 200 ns rising time was lower than that with 2 μs. In order to obtain a given inactivation effect, treatment time required for the rectangular pulse with 200 ns rise time was shorter than that with 2 μs.