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23 results on '"Jiang, Linglei"'

1. ELISA‐based detection of immunoglobulins against extracellular vesicles in blood plasma.

Author

Driedonks, Tom A. P., Jiang, Linglei, Gololobova, Olesia, Liao, Zhaohao, and Witwer, Kenneth W.

Subjects
*EXTRACELLULAR vesicles, *BLOOD plasma, *IMMUNOGLOBULINS, *ANTIBODY formation, *IMMUNE response
Abstract

Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell‐derived EVs into pig‐tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV‐mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV‐based therapies in preclinical studies. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Vaccines’ New Era-RNA Vaccine

Author

Zhou, Wenshuo, primary, Jiang, Linglei, additional, Liao, Shimiao, additional, Wu, Feifei, additional, Yang, Guohuan, additional, Hou, Li, additional, Liu, Lan, additional, Pan, Xinping, additional, Jia, William, additional, and Zhang, Yuntao, additional

Published
2023
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3. Bacterial extracellular vesicles: A position paper by the microbial vesicles task force of the Chinese society for extracellular vesicles

Author

Wen, Minghui, primary, Wang, Jingyu, additional, Ou, Zihao, additional, Nie, Guangjun, additional, Chen, Ye, additional, Li, Min, additional, Wu, Zhiwei, additional, Xiong, Sidong, additional, Zhou, Hongwei, additional, Yang, Zifeng, additional, Long, Gang, additional, Su, Jiacan, additional, Liu, Han, additional, Jin, Yingying, additional, Wen, Zhenke, additional, Fu, Yuxuan, additional, Zhou, Tieli, additional, Xie, Hui, additional, Guan, Wenda, additional, Sun, Xi, additional, Wang, Zhang, additional, Wang, Juan, additional, Chen, Xunxun, additional, Jiang, Linglei, additional, Qin, Xiaolin, additional, Xue, Yicong, additional, Huang, Mei, additional, Huang, Xinyue, additional, Pan, Ruoru, additional, Zhen, Haojun, additional, Du, Yuqi, additional, Li, Qianbei, additional, Huang, Xixin, additional, Wu, Yuanyuan, additional, Wang, Pingping, additional, Zhao, Kening, additional, Situ, Bo, additional, Hu, Xiumei, additional, and Zheng, Lei, additional

Published
2023
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4. Pharmacokinetics and biodistribution of extracellular vesicles administered intravenously and intranasally to Macaca nemestrina

Author

Driedonks, Tom, primary, Jiang, Linglei, additional, Carlson, Bess, additional, Han, Zheng, additional, Liu, Guanshu, additional, Queen, Suzanne E., additional, Shirk, Erin N., additional, Gololobova, Olesia, additional, Liao, Zhaohao, additional, Nyberg, Lyle H., additional, Lima, Gabriela, additional, Paniushkina, Liliia, additional, Garcia‐Contreras, Marta, additional, Schonvisky, Kayla, additional, Castell, Natalie, additional, Stover, Mitchel, additional, Guerrero‐Martin, Selena, additional, Richardson, Riley, additional, Smith, Barbara, additional, Mahairaki, Vasiliki, additional, Lai, Charles P., additional, Izzi, Jessica M., additional, Hutchinson, Eric K., additional, Pate, Kelly A. M., additional, and Witwer, Kenneth W., additional

Published
2022
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6. A bacterial extracellular vesicle‐based intranasal vaccine against SARS‐CoV‐2 protects against disease and elicits neutralizing antibodies to wild‐type and Delta variants

Author

Jiang, Linglei, primary, Driedonks, Tom A.P., additional, Jong, Wouter S.P., additional, Dhakal, Santosh, additional, Bart van den Berg van Saparoea, H., additional, Sitaras, Ioannis, additional, Zhou, Ruifeng, additional, Caputo, Christopher, additional, Littlefield, Kirsten, additional, Lowman, Maggie, additional, Chen, Mengfei, additional, Lima, Gabriela, additional, Gololobova, Olesia, additional, Smith, Barbara, additional, Mahairaki, Vasiliki, additional, Riley Richardson, M., additional, Mulka, Kathleen R., additional, Lane, Andrew P., additional, Klein, Sabra L., additional, Pekosz, Andrew, additional, Brayton, Cory, additional, Mankowski, Joseph L., additional, Luirink, Joen, additional, Villano, Jason S., additional, and Witwer, Kenneth W., additional

Published
2022
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8. A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles

Author

Jiang, Linglei, Luirink, Joen, Kooijmans, Sander A A, van Kessel, Kok P M, Jong, Wouter, van Essen, Max, Seinen, Cor W, de Maat, Steven, de Jong, Olivier G, Gitz-François, Jerney F F, Hennink, Wim E, Vader, Pieter, Schiffelers, Raymond M, Pharmaceutics, Afd Pharmaceutics, Molecular Microbiology, AIMMS, LaserLaB - Molecular Biophysics, Pharmaceutics, and Afd Pharmaceutics

Subjects
Surface Properties, Blotting, Western, Biophysics, Cell Culture Techniques, Outer membrane vesicles, 02 engineering and technology, Extracellular vesicles, Biochemistry, Post insertion, 03 medical and health sciences, Mice, Microscopy, Electron, Transmission, Labelling, Cell Line, Tumor, Animals, Humans, Molecular Biology, 030304 developmental biology, 0303 health sciences, Liposome, Bacteria, Chemistry, Translation (biology), 021001 nanoscience & nanotechnology, Flow Cytometry, Poly(ethylene glycol)lipids, Immunohistochemistry, Cell biology, Bacterial Outer Membrane, HEK293 Cells, Post-insertion, Nucleic acid, Surface modification, Electrophoresis, Polyacrylamide Gel, 0210 nano-technology, Drug carrier
Abstract

Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a ‘post-insertion’ approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.

Published
2021

9. Pharmacokinetics and biodistribution of extracellular vesicles administered intravenously and intranasally to Macaca nemestrina

Author

Driedonks, Tom, primary, Jiang, Linglei, additional, Carlson, Bess, additional, Han, Zheng, additional, Liu, Guanshu, additional, Queen, Suzanne E., additional, Shirk, Erin N., additional, Gololobova, Olesia, additional, Nyberg, Lyle H., additional, Lima, Gabriela, additional, Paniushkina, Liliia, additional, Garcia-Contreras, Marta, additional, Schonvisky, Kayla, additional, Castell, Natalie, additional, Stover, Mitchel, additional, Guerrero-Martin, Selena, additional, Richardson, Riley, additional, Smith, Barbara, additional, Mahairaki, Vasiliki, additional, Lai, Charles P., additional, Izzi, Jessica M., additional, Hutchinson, Eric K., additional, Pate, Kelly A.M., additional, and Witwer, Kenneth W., additional

Published
2021
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10. A bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants

Author

Jiang, Linglei, primary, Driedonks, Tom A.P., additional, Jong, Wouter S.P., additional, Dhakal, Santosh, additional, Berg van Saparoea, H. Bart van den, additional, Sitaras, Ioannis, additional, Zhou, Ruifeng, additional, Caputo, Christopher, additional, Littlefield, Kirsten, additional, Lowman, Maggie, additional, Chen, Mengfei, additional, Lima, Gabriela, additional, Gololobova, Olesia, additional, Smith, Barbara, additional, Mahairaki, Vasiliki, additional, Richardson, M. Riley, additional, Mulka, Kathleen R., additional, Lane, Andrew P., additional, Klein, Sabra L., additional, Pekosz, Andrew, additional, Brayton, Cory F., additional, Mankowski, Joseph L., additional, Luirink, Joen, additional, Villano, Jason S., additional, and Witwer, Kenneth W., additional

Published
2021
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21. Thermodynamic and kinetic investigation of agomelatine polymorph transformation.

Author

Zhang, Qi, Jiang, Linglei, and Mei, Xuefeng

Subjects
ACTIVATION energy, CHEMICAL kinetics, POTENTIAL energy, POLYMORPHISM (Crystallography), X-ray powder diffraction
Abstract

Thermodynamic properties of polymorphic forms I and II of Agomelatine were investigated and the bimorphism was determined to be monotropically related. The phase transition kinetics from metastable form I to thermodynamically stable form II was studied and a quantification method was developed based on X-ray powder diffraction technique. Various solid-state kinetic models were examined and the results were fit to the experimental data. The nucleation kinetic models were found to be the best fit to describe the experimental data across the temperature range. The activation energy of the form transformation was calculated in the range of 116–122 kJ mol−1, irrespective of which kinetic model selected. [ABSTRACT FROM AUTHOR]

Published
2016
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22. A bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants.

Author

Jiang L, Driedonks TAP, Jong WSP, Dhakal S, van den Berg van Saparoea HB, Sitaras I, Zhou R, Caputo C, Littlefield K, Lowman M, Chen M, Lima G, Gololobova O, Smith B, Mahairaki V, Richardson MR, Mulka KR, Lane AP, Klein SL, Pekosz A, Brayton CF, Mankowski JL, Luirink J, Villano JS, and Witwer KW

Abstract

Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Current SARS-CoV-2 vaccines include mRNA-containing lipid nanoparticles or adenoviral vectors that encode the SARS-CoV-2 Spike (S) protein of SARS-CoV-2, inactivated virus, or protein subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed in the future to address issues such as stability and storage requirements, need for vaccine boosters, desirability of different routes of administration, and emergence of SARS-CoV-2 variants such as the Delta variant. Here, we present a novel, well-characterized SARS-CoV-2 vaccine candidate based on extracellular vesicles (EVs) of Salmonella typhimurium that are decorated with the mammalian cell culture-derived Spike receptor-binding domain (RBD). RBD-conjugated outer membrane vesicles (RBD-OMVs) were used to immunize the golden Syrian hamster ( Mesocricetus auratus ) model of COVID-19. Intranasal immunization resulted in high titers of blood anti-RBD IgG as well as detectable mucosal responses. Neutralizing antibody activity against wild-type and Delta variants was evident in all vaccinated subjects. Upon challenge with live virus, hamsters immunized with RBD-OMV, but not animals immunized with unconjugated OMVs or a vehicle control, avoided body mass loss, had lower virus titers in bronchoalveolar lavage fluid, and experienced less severe lung pathology. Our results emphasize the value and versatility of OMV-based vaccine approaches.

Published
2022
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23. A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles.

Author

Jiang L, Luirink J, Kooijmans SAA, van Kessel KPM, Jong W, van Essen M, Seinen CW, de Maat S, de Jong OG, Gitz-François JFF, Hennink WE, Vader P, and Schiffelers RM

Subjects
Animals, Bacterial Outer Membrane ultrastructure, Blotting, Western methods, Cell Culture Techniques methods, Cell Line, Tumor, Electrophoresis, Polyacrylamide Gel methods, Extracellular Vesicles ultrastructure, Flow Cytometry methods, HEK293 Cells, Humans, Mice, Microscopy, Electron, Transmission methods, Surface Properties, Bacteria chemistry, Bacterial Outer Membrane chemistry, Extracellular Vesicles chemistry, Immunohistochemistry methods
Abstract

Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a 'post-insertion' approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2021
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