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Myocardial damage is a critical complication and a significant contributor to mortality in sepsis. MicroRNAs (miRNAs) have emerged as key players in sepsis pathogenesis. In this study, we explore the effect and mechanisms of miR-29b-1-5p on sepsis-induced myocardial damage. Sepsis-associated Gene Expression Omnibus datasets (GSE72380 and GSE29914) are examined for differential miRNAs. The mouse sepsis-induced cardiac injury was established by Lipopolysaccharide (LPS) or cecal ligation and puncture (CLP). LPS-treated HL-1 mouse cardiomyocytes simulate myocardial injury in vitro. miR-29b-1-5p is co-upregulated in both datasets and in cardiac tissue from sepsis mouse and HL-1 cell models. miR-29b-1-5p expression downregulation was achieved by antagomir transduction and confirmed by real-time quantitative reverse transcription PCR. Survival analysis and echocardiography examination show that miR-29b-1-5p inhibition improves mice survival cardiac function in LPS- and CLP-induced sepsis mice. Hematoxylin and eosin and Masson’s trichrome staining and Immunohistochemistry analysis of mouse myocardial α-smooth muscle actin show that miR-29b-1-5p inhibition reduces myocardial tissue injury and fibrosis. The inflammatory cytokines and cardiac troponin I (cTnI) levels in mouse serum and HL-1 cells are also decreased by miR-29b-1-5p inhibition, as revealed by enzyme-linked immunosorbent assay. The expressions of autophagy-lysosomal pathway-related and apoptosis-related proteins in the mouse cardiac tissues and HL-1 cells are evaluated by western blot analysis. The sepsis-induced activation of the autophagy-lysosomal pathway and apoptosis are also reversed by miR-29b-1-5p antagomir. MTT and flow cytometry measurement further confirm the protective role of miR-29b-1-5p antagomir in HL-1 cells by increasing cell viability and suppressing cell apoptosis. Metascape functionally enriches TargetScan-predicted miR-29b-1-5p target genes. TargetScan prediction and dual luciferase assay validate the targeting relationship between miR-29b-1-5p and telomeric repeat-binding factor 2 (TERF2). The expression and function of TERF2 in HL-1 cells and mice are also evaluated. MiR-29b-1-5p negatively regulates the target gene TERF2. TERF2 knockdown partly restores miR-29b-1-5p antagomir function in LPS-stimulated HL-1 cells. In summary, miR-29b-1-5p targetedly inhibits TERF2, thereby enhancing sepsis-induced myocardial injury.

The evolution of inclusions in molten steel in tundish before and after vacuum treatment during the production of GCr15 bearing steel in a steel plant is analyzed， the results showed that total oxygen content in molten steel increased greatly in tundish. Magnesium aluminum Spinel inclusions increased in RH soft blowing and tundish to varying degrees， and the number increased significantly in tundish. The MgO aggregate particles in the working layer of the tundish coating are greatly reduced， and some MgO particles are dissolved in the molten steel， resulting in the increase of oxygen content in the molten steel.The effect of different SiO2 content on the phase of tundish coating was discussed by thermodynamic simulation analysis. It was proposed that the control of SiO2 content at range 4%~6% and the strengthening of tundish masonry management can reduce the formation of high temperature low melting phase in tundish coating and effectively improve the flaw detection eligibility rate of bearing steel GCr15 head slab.

One of the important requirements for converter steelmaking is to accurately calculate the amount of lime added based on the conditions of hot metal and the requirements of molten steel， in order to avoid waste of flux. However， due to the fast pace of converter production and multiple influencing factors， it is difficult to accurately determine the amount of lime added. In order to accurately calculate the amount of converter lime， avoid lime waste and reduce unnecessary steelmaking cost， mechanism model， classification model and multiple linear regression model are established to calculate the amount of converter lime. Compared with the three models， the pure mechanism model has low calculation accuracy and is difficult to be directly applied to field production. According to the classification model optimized by mechanism model， only 60% of the furnace times difference is less than 500 kg/ furnace. A multiple linear regression model is established using SPSS， and the relationship between lime addition amount and other parameters is obtained WLime=4 147.24×wTSi+529.67×wTC+60.20×Wqf+0.405×VO-1 833.95. The verification results show that， 93% of the calculated difference of the furnace is less than 400 kg/ furnace， 77% of the calculated difference of the furnace is less than 300 kg/ furnace， which can basically meet the requirements of field use. After the model is applied in the field， the amount of lime added is effectively reduced.

Background: Neuroblastoma (NBL) is an extracranial malignant tumor in children deriving from the neural crest in the sympathetic nervous system. Although various immunotherapy interventions have made significant breakthroughs in many adult cancers, the efficacy of these immunotherapies was still limited in NBL. NBL has low immunogenicity which results in a lack of tumor-infiltrating T lymphocytes in the tumor microenvironment (TME). Moreover, tumor cells can wield many immune evasion strategies both in the TME and systemically to impede lymphocyte infiltration and activation. All these factors hamper the anti-tumor effects of CD8+ T cells during immunotherapy and the levels of infiltrating CD8+ T cells correlate with therapy response. Materials and methods: In this study, we utilized multidimensional bioinformatic methods to establish a risk model based on CD8+ T cells -related genes (CD8+ TRGs). Results: We obtained 33 CD8+ TRGs with well-predictive ability for prognosis in both GSE49711 and E-MTAB-8248 cohorts. Then, 12 CD8+ TRGs including HK2, RP2, HPSE, ELL2, GFI1, SLC22A16, FCGR3A, CTSS, SH2D1A, RBP5, ATF5, and ADAM9 were finally identified for risk model construction and validation. This model revealed a stable performance in prognostic prediction of the overall survival (OS) and event-free survival (EFS) in patients with NBL. Additionally, our research indicated that the immune and stromal scores, immune-related pathways, immune cell infiltration, the expression of major histocompatibility complex (MHC) and immune checkpoint molecules, immunotherapy response, and drug susceptibility revealed significant differences between high and low-risk groups. Conclusions: According to our analyses, the constructed CD8+ TRGs-based risk model may be promising for the clinical prediction of anti-tumor therapy responses and prognoses in NBL. [ABSTRACT FROM AUTHOR]

In order to analyze the effect of cold joints on the interlayer bonding property of 3DPC and the formation mechanism of interlayer cold joints, the interlayer bonding property of a 3D printing cementitious material (3DPC) was evaluated by direct tensile test, and the process of interlayer cold joints extension of 3DPC was simulated by near field dynamic method. The results indicate that there is no cold joint between printed layers when the printing time of the upper layer is less than the starting time of the hydration induction period of cement, and the upper layer printing time and dosage of nano-clay have little effect on the interlayer bonding strength. After the upper layer printing time exceeds the starting time of cement hydration induction period, the number, continuity and length of interlayer cold joints increase with the prolongation of interlayer cycle time, and the interlayer bonding strength decreases, but the interlayer bonding strength can be significantly improved by adding nanoclay. The cold joint of 3DPC can be prevented and the interlayer bonding can be enhanced by controlling the upper layer printing time and by adding nano-clay to ensure proper relative humidity between 3DPC interlayer. [ABSTRACT FROM AUTHOR]

Ta2N is an effective diffusion barrier material to prevent undesired Cu diffusion in ultra-large scale integration circuits. Previous theoretical work has reported the interesting result that at the Cu/Ta2N interface the Cu layer preferentially bonded with the Ta layer but not the N layer of Ta2N. However, this result was calculated from largely lattice mismatched interface models. To confirm this theoretical result and unravel the cause of strong Cu–Ta bonding at the Cu/Ta2N interface, in this study density functional theory calculations, on the basis of super-cell models, were performed to investigate the Cu(111)/Ta2N(001) interface. We firstly calculated interface cohesive energies and confirmed that the Cu layer preferentially bonded with the Ta layer of Ta2N. Then, electronic structure calculations revealed that the chemical bonding of the Cu–Ta bond at the Cu(111)/Ta2N(001) interface was primarily covalent in character, providing a proper explanation for the close integration of the Cu layer and Ta layer. Lastly, Cu diffusion investigations revealed that Ta2N was able to effectively prevent Cu diffusion. Furthermore, we found that the N layer of Ta2N played the critical role in preventing Cu diffusion. [ABSTRACT FROM AUTHOR]