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【Objective】According to the market's demand for taste-type and fresh-eatable cabbage, more varieties of cabbage with good characters and in line with market supply and demand were selected by using its own germplasm resources.【Method】Using 13 Ougra sterile heading cabbage materials selected by the team as female parents and 10 inbred lines heading cabbage materials (GL14000-14009) as male parents, 14 heading cabbage hybrid combinations were prepared through hybridization, the experiments were compared and screened from the aspects of plant width, outer leaf number, longitudinal diameter, transverse diameter, central column length, yield and disease resistance.【Result】After comparison, GL22-02, GL22-06, GL22-11 and GL22-13 were obtained, and their agrtural traits, quality and yield were better than those of the control Tejia No. 2. All the leaf bulbs were round, and the petiole-leaf ratio was 26.9%, 20.8%, 23.1% and 16.8%. It was better than that of control variety (31.3%). Leaf bulb compactness was 0.76, 0.73, 0.59 and 0.62 g/cm3, which was more than 0.16 g/cm3 higher than that of the control variety. The central column length was less than half of the leaf bulb height, 4.9, 4.3, 4.8 and 4.6 cm, respectively. The moisture contents were 93.22%, 94.17%, 93.75% and 93.61%, protein contents were 1.51%, 1.28%, 1.39% and 1.36%, respectively. The soluble sugar content were 5.61%, 5.69%, 5.56% and 5.84%, respectively, which were 0.14-0.37 percentage point higher than that of the control variety. The crude cellulose content were 0.61%, 0.71%, 0.69% and 0.67%, respectively, which were 0.08-0.18 percentage point lower than that of the control variety. The vitamin C contents were 682.3, 687.1, 699.1 and 682.3 mg/kg, respectively, which were 48.4-65.2 mg/kg higher than the control variety. After edible quality identification, four hybrid materials of heading cabbage were selected, the leaf balls were raw (fresh) to eat, crisp texture, sweet taste. The black rot disease resistance of the composite materials was high (HR), the same as that of the control.The yield was 4.80%, 8.46%, 10.97% and 8.46% higher than that of control Tejia No. 2.【Conclusion】The four composite materials GL22-02, GL22-06, GL22-11 and GL22-13 were selected. They both had agronomic and economic characters, which can be used for production demonstration in large scale.

Abstract Background Chinese cabbage is one of the most widely grown leafy vegetables in China. Cytoplasmic male sterility (CMS) is a maternally inherited trait that produces abnormal pollen during anther development, which is commonly seen in cruciferous vegetables. However, the molecular mechanism of Chinese cabbage CMS is not clear. In this study, the metabolome and hormone profiles of Chinese cabbage male sterile line (CCR20000) and sterile maintainer line (CCR20001) were analyzed in flower buds during normal stamen development and abnormal stamen development, respectively. Results A total of 556 metabolites were detected based on UPLC-MS/MS detection platform and database search, and the changes of hormones such as auxin, cytokinins, abscisic acid, jasmonates, salicylic acid, gibberellin acid and ethylene were analyzed. The results showed that compared with the male fertile line (MF), the male sterile line (MS) significantly decreased the content of flavonoids and phenolamides metabolites in the stamen dysplasia stage, accompanied by a large accumulation of glucosinolate metabolites. Meanwhile, the contents of GA9, GA20, IBA, tZ and other hormones in MS were significantly lower than those in MF strains. Further, by comparing the metabolome changes of MF and MS during stamen dysplasia, it was found that flavonoid metabolites and amino acid metabolites were distinctly different. Conclusions These results suggest that flavonoids, phenolamides and glucosinolate metabolites may be closely related to the sterility of MS strains. This study provides an effective basis for further research on the molecular mechanism of CMS in Chinese cabbage.

Background: COVID-19 is characterized by endothelial dysfunction and is presumed to have long-term cardiovascular sequelae. In this study, we aimed to explore the serum levels of endothelial biomarkers of COVID-19 recovers 1-year after hospital discharge. Methods: A clinical follow-up study, including 345 COVID-19 survivors from Huanggang, Hubei, and 119 age and gender matched healthy controls were enrolled in the study. A standardized symptom questionnaire, electrocardiogram and Doppler ultrasound of lower extremities, routine blood tests, biochemical and immunological tests were collected, and serum levels of soluble vascular cell adhesion molecule-1(VCAM-1), intercellular cell adhesion molecule-1(ICAM-1), P-selectin, and fractalkine were measured by enzyme-linked immunosorbent assays. Results: 1-year after discharge, 39% of recovers possessed post-COVID syndromes, while no deep vein thrombosis was detected in all screened. No significant differences in circulatory inflammatory markers (leukocytes, neutrophils, lymphocytes, C-reactive protein and interleukin-6), alanine aminotransferase, estimated glomerular filtration rate, glucose, triglycerides, total cholesterol and D-dimer observed among controls and previously mild or severe infected. Furthermore, serum levels of VCAM-1, ICAM-1, P-selectin, and fractalkine presented no significant differences between survivors and healthy controls. Conclusions: SARS-CoV-2 infection may not impose a higher risk of long-term cardiovascular sequelae, even for those recovered from severe illness. KEYWORDS: COVID, ENDOTHELIAL DYSFUNCTION, INFLAMMATORY MARKERS, ADHESION MOLECULES, THROMBOSIS.

Objective:This study investigated the COVID-19-prevention knowledge and practices of healthcare workers (HCWs), their psychological states concerning the return to work, and their trust and requirements in using traditional Chinese medicine (TCM) to prevent and treat COVID-19. It is hoped that the study can serve as a reference for policy making during the resumption of work in other countries or regions experiencing similar situations.Methods:This study comprised a quantitative cross-sectional online survey design. Purposive sampling and Cluster sampling were used to recruit all HCWs working in public hospitals in Huangzhou District, Huanggang City, Hubei Province, China. From April 23 to May 14, 2020, surveys were sent electronically to all 13 public hospitals in this area.Results:In total, 2,079 responses were received and 2,050 completed forms were included. After analysis, 47.9 and 46.6% of HCWs indicated that they possessed very good knowledge or good knowledge of preventative measures, respectively. Multivariable log-binomial regression indicated that male, tertiary hospital, medical staff, and undergraduate/postgraduate qualification were associated with good knowledge. Good knowledge was also well-correlated with good practice (OR: 3.277; 95% CI: 2.734–3.928; P < 0.01). 59.8% of HCWs reported worries about resuming work; especially asymptomatic infections. The Self-Rating Anxiety Scale (SAS) indicated that 10.8% of participants had mild anxiety, 1.5% moderate anxiety, and 0.1% severe anxiety. Female, divorced/widowed, and working in a high risk hospital (the Huangzhou District People's Hospital was used for throat swab examinations of returning workers) were risk factors for concerns about resuming work and anxiety symptoms. However, good preventive knowledge was a protective factor for anxiety. HCWs' trust in using TCM to treat COVID-19 was significantly higher than their trust in using TCM for prevention (P < 0.001). Regarding preferences for preventative TCM products, oral TCM granules were the most preferred (62.4%). HCWs also indicated they wanted to know more about the clinical efficacy, applicable population, and adverse reactions of preventative TCM products (89.3, 81.1, and 81.4%, respectively).Conclusion:While HCWs had good knowledge of COVID-19 preventative measures, this did not eliminate the psychological impact of resumption of work. Promotion of COVID-19 prevention knowledge reduces the risk of infection, and alleviates the worries and anxiety symptoms of HCWs about resuming work (especially in administrative staff, those with low education, and those working in primary hospitals). Additional psychological support is required for female HCWs, divorced/widowed HCWs, and those working in high-risk hospitals. Finally, systematic trials of preventative TCM products are recommended.

Objective: The objective of this study was to investigate how knowledge and practice of coronavirus disease 2019 (COVID-19) prevention measures affected concerns about returning to work among supermarket staff. Attitudes about the ability of traditional Chinese medicine (TCM) to prevent COVID-19 were also assessed.Methods: A cross-sectional study was conducted in Huanggang, Hubei Province, China from April 23 to 25, 2020. Participants were invited to fill out an electronic questionnaire on their cell phones.Results: The results showed that from 2,309 valid questionnaires, 61.5% of participants were concerned about resuming work. Major concerns included asymptomatic infection (85.01%) and employees gathering in the workplace (78.96%). Multivariate logistic regression indicated that the female gender, having school-aged children and pregnancy were risk factors for being concerned about resuming work, while good knowledge and practice of preventive measures were protective factors. Knowledge and practice of preventive measures were positively correlated. Among preventive measures, the highest percentage of participants knew about wearing masks and washing hands. Meanwhile, 65.8% of participants expressed confidence in the ability of TCM to prevent COVID-19, where 74 and 51.3% thought there was a need and a strong need, respectively, for preventive TCM-based products. Among them, 71.5% preferred oral granules. Regarding TCM as a COVID-19 preventative, most were interested in information about safety and efficacy.Conclusion: These findings suggested that promoting knowledge and practices regarding COVID-19 prevention can help alleviate concerns about returning to work. Meanwhile, TCM can feasibly be accepted to diversify COVID-19 prevention methods.Clinical Trial Registration:http://www.chictr.org.cn/, identifier: ChiCTR2000031955.

Male sterility exists widely in flowering plants and is used as a fascinating tool by breeders for creating hybrid varieties. Herein, stamen samples from male sterile CCR20000 and male fertile CCR20001 lines during two developmental stages were employed to elucidate the molecular changes during flower development in fertile and sterile Chinese cabbage lines. RNA-seq revealed weak transcriptional activity in the sterile line, which may have led to the abnormal stamen development. The differentially expressed genes were enriched in plant hormone, carbon metabolism, and biosynthesis of amino acid pathways. Important genes with opposite patterns of regulation between the two lines have been associated with the male sterility trait. Members of the transcription factor families such as AP2, MYB, bHLH, and WRKY were highly active in the regulation of structural genes involved in pollen fertility. This study generated important genomic information to support the exploitation of the male sterility trait in Chinese cabbage breeding programs.

To develop a new kind of famotidine-resin microcapsule for gastric adhesion sustained release by screening out suitable excipients and designing reasonable prescriptions to improve patient drug activities to achieve the expected therapeutic effect. The famotidine drug resin was prepared using the water bath method with carbomer 934 used as coating material. Microcapsules were prepared using the emulsified solvent coating method and appropriate excipients were used to prepare famotidine sustained release suspension. Pharmacokinetics of the developed microcapsules were studied in the gastrointestinal tract of rats. The self-made sustained-release suspension of famotidine hydrochloride effectively reduced the blood concentration and prolonged the action time. The relative bioavailability of the self-made suspension of the famotidine hydrochloride to the commercially available famotidine hydrochloride was 146.44%, with an average retention time of about 5h longer, which indicated that the new suspension had acceptable adhesion properties. The findings showed that the newly developed famotidine-resin microcapsule increased the bioavailability of the drug with a significant sustained-release property. [ABSTRACT FROM AUTHOR]

The durability of infection-induced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity has crucial implications for reinfection and vaccine effectiveness. However, the relationship between coronavirus disease 2019 (COVID-19) severity and long-term anti-SARS-CoV-2 immunoglobulin G (IgG) antibody level is poorly understood. Here, we measured the longevity of SARS-CoV-2-specific IgG antibodies in survivors who had recovered from COVID-19 1 year previously. In a cohort of 473 survivors with varying disease severity (asymptomatic, mild, moderate, or severe), we observed a positive correlation between virus-specific IgG antibody titers and COVID-19 severity. In particular, the highest virus-specific IgG antibody titers were observed in patients with severe COVID-19. By contrast, 74.4% of recovered asymptomatic carriers had negative anti-SARS-CoV-2 IgG test results, while many others had very low virus-specific IgG antibody titers. Our results demonstrate that SARS-CoV-2-specific IgG persistence and titer depend on COVID-19 severity. [ABSTRACT FROM AUTHOR]

Different sterile cytoplasm types of nine cabbage cytoplasmic male sterile materials were identified by molecular marker in the study, in order to better use molecular marker to conduct the assisted breeding in the future. Genomic DNA was isolated from Chinese cabbage by CTAB method. The design of two pairs of specific primers was performed on conserved flanking region of orfl38 gene in the GenBank. PCR was performed with genomic DNA of the nine Chinese cabbage materials. The bands were sequenced. The homologous comparison was conducted in NCBI, and finally, the type of sterile cytoplasm was determined. The results showed that the bands were amplified only in four Chinese cabbage male sterile materials with two pairs of specific primers PI/PR and PIII/PIV, while the other five materials did not obtain the relative bands. The result was consistent with the field sterility identification. And then four molecular markers of Chinese cabbage Ogura cytoplasmic male sterility (CMS) were obtained. After conducting a homologous comparative analysis with BLAST in GenBank, it was found that the homologous degree was 100% in specific segments of the three sterility materials (LI-CI, L3-CI and L3-F l) and Ogu orfl38 gene (GenBank accession No.: HQ149728) of the reported broccoli Ogu CMS. The homologous degree of LI-Fl was 99% with a variation point. The type of cytoplasmic male sterility of the other five materials needed further research. Four materials of the nine were identified as the radish cytoplasmic male sterility materials and four molecular markers were obtained. [ABSTRACT FROM AUTHOR]

Isolated microspore culture was carried out with Brassica juncea to study the effects of sterilization methods, culture period of embryos, hormone formula and the concentration of activated carbon on embryogenesis, and the concentration of NAA on induction of roots. The results showed that the embryogenesis on medium subjected to filter sterilization is better than medium subjected to autoclaved sterilization. The embryos cultured for 15 d had the highest plantlet regeneration rate (52%). NLN-13 liquid medium including 0.1 mg/L 6-BA and CL 2 g/L activated carbon significantly improved the plantlet regeneration rate. 1/2 MS including 0.3 mg/L NAA had the highest plantlet regeneration rate (100%). [ABSTRACT FROM AUTHOR]

In this study, using Japanese radish cytoplasmic sterile line "Half Green Cabbage" (HGC) and clubroot-resistant South Korea cabbage variety "CR Cabbage King" (CCK) as experimental materials, self-incompatible DH lines were obtained by microspore culture. Through five generations of backcross, CCR11239, a clubroot-resistant radish cytoplasmic sterile line, was obtained. Through six generations of self-crossing, CCR11240, the maintainer line of CCR11239, was bred. After the cross of CCK and Luchunbai 1 (83-1) and five generations of self-crossing, self-compatible line CCR11241 was obtained. Through crossing CCR11240 with CCR11241, the new cabbage variety CCR11242 was obtained. After variety comparison test, provincial regional test and production demonstration test, the new variety was registered in 2012. The average yield of CCR11242 reached 60 975 kg/hm2, which was improved by 142% compared with Luchunbai 1 (83 - 1). The disease index of CCR11242 was 5.63, which was 88.13 lower than the control, indicating high resistance (HR) to clubroot. [ABSTRACT FROM AUTHOR]