1. Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition
- Author
-
Bi Ma, Jianqiong Hu, Wuqi Wei, Jianglian Yuan, Ningjia He, Yiwei Luo, Hongshun Li, Haiye Hao, and Zhiyuan Lv
- Subjects
0106 biological sciences ,0301 basic medicine ,Aging ,Hydrastis ,vegetative phase transition ,age pathway ,Perennial plant ,QH301-705.5 ,Transgene ,Arabidopsis ,Biology ,Plant Roots ,perennial woody plants ,01 natural sciences ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Arabidopsis thaliana ,Biology (General) ,Physical and Theoretical Chemistry ,Promoter Regions, Genetic ,QD1-999 ,Molecular Biology ,Spectroscopy ,Plant Proteins ,Organic Chemistry ,Computational Biology ,Gene Expression Regulation, Developmental ,General Medicine ,Plants, Genetically Modified ,biology.organism_classification ,mir156s ,Yeast ,Up-Regulation ,Computer Science Applications ,Cell biology ,Plant Leaves ,MicroRNAs ,mulberry ,Chemistry ,Populus ,030104 developmental biology ,Molecular mechanism ,Morus ,Transcription Factors ,010606 plant biology & botany ,Woody plant - Abstract
The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in Arabidopsis thaliana. The over-expression of miR156 in transgenic Populus × canadensis dramatically prolongs the juvenile phase. Here, we find that the expression of mno-miR156 decreases with age in all tissues in mulberry, which led us to study the hierarchical action of miR156 in mulberry. Utilizing degradome sequencing and dual-luciferase reporter assays, nine MnSPLs were shown to be directly regulated by miR156. The results of yeast one-hybrid and dual-luciferase reporter assays also revealed that six MnSPLs could recognize the promoter sequences of mno-miR172 and activate its expression. Our results demonstrate that mno-miR156 performs its role by repressing MnSPL/mno-miR172 pathway expression in mulberry. This work uncovered a miR156/SPLs/miR172 regulation pathway in the development of mulberry and fills a gap in our knowledge about the molecular mechanism of vegetative phase transition in perennial woody plants.
- Published
- 2021
- Full Text
- View/download PDF