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Abstract Numerous studies have found that some food preservatives are hazardous to human health and can lead to cancer in the long run. Plant extracts are becoming increasingly popular and garnering the attention of researchers due to their important antibacterial and antioxidant components. In this review, we will focus on some chemical preservatives, the potential health risks of nitrate and nitrite, sodium benzoate, and potassium sorbate in food processing. Natural plant extracts are also discussed as antimicrobials and antioxidants, as well as their potent roles as medicinal plants and antimicrobial agents that can be employed to reduce the percentage of food preservatives. Data reveals that synthetic chemicals in food additives can promote obesity, cancer, asthma, and heart problems. Natural plant extracts have antibacterial and antioxidant characteristics, making them a partially viable alternative to synthetic preservatives. Bioactive compounds, agro-industrial processes, and green processing technologies that are environmentally friendly are important for improving food products.

Abstract This study conducted a comparison of the nutritional content and lipid composition of five different varieties of quinoa (QL-1, SJ-1, SJ-2, KL-1, and KL-2) from Qinghai Province, China. Each of the five varieties exhibited varying levels of essential nutrients, including crude protein, dietary fiber, and crude fat. The QL-1 variety has the highest concentration of phytic acid, measuring 1.66 g/100 g. A non-targeted lipomics analysis discovered a total of 16 lipid categories and 383 individual lipids in quinoa. Out of the several substances, glyceride had the highest concentration, exceeding 70%, with phospholipids coming next. The percentage of fatty acids and fatty acid esters was between 5 and 11%. The glycerides in all five kinds exhibited a similar composition, with unsaturated glycerides constituting over 99% of the total glycerides. Phospholipids constituted over 11% of the overall lipid content, with lecithin comprising more than 80% and cephalin ranging from 16.70 to 18.61%. The results establish a solid basis for the utilization of quinoa in processing, particularly in lipid processing. Graphical Abstract

ABSTRACT During September to December 2021, school mask policies to mitigate SARS-CoV-2 transmission varied throughout the US. We compared infection-induced seroprevalence estimates and estimated seroconversion among children residing in areas with and without school mask requirements. We estimated infection-induced seroprevalence among children in three age groups (5–17, 5–11, and 12–17 years) in areas with and without school district mask requirements for two time points: September 1–30, 2021 and December 15, 2021 to January 14, 2022. Robust Poisson regression models estimated population seroconversion over the semester among initially seronegative children. Permutation tests assessed for significant differences in the estimated population seroconversion due to incident infections by school district mask policy. Residing in an area with no school mask requirement was associated with higher infection-induced seroprevalence among children aged 5–17 years (adjusted prevalence ratio [aPR] = 1.18, 95% confidence interval [CI]: 1.10, 1.26), and those aged 5–11 years (aPR) = 1.21, 95% CI: 1.10, 1.32) and those aged 12–17 years (aPR = 1.16, 95% CI: 1.07, 1.26), compared with areas requiring masks in school. Estimated population seroconversion during the semester was also significantly higher among children in districts without mask policies than those with school mask requirements among all age groups (5–17 years: 23.7% vs 18.1%, P < 0.001; 5–11 years: 6.4% vs 4.5%, P = 0.002;12–17 years: 27.2% vs 21.0%, P < 0.001). During the U.S. Fall 2021 semester, areas with school mask requirements had lower infection-induced seroprevalence and an estimated lower proportion of seroconversion due to incident infection among school-aged children compared with areas without school mask requirements; causality cannot necessarily be inferred from these associations.IMPORTANCEDuring the U.S. Fall 2021 school semester, the estimated proportion of previously uninfected school-aged children who experienced a first infection with SARS-CoV-2 was lower in areas where public school district policies required masks for all staff and students compared with areas where the school districts had no mask requirements. Because children are more likely than adults to experience asymptomatic or mild SARS-CoV-2 infections, the presence of infection-induced antibodies is a more accurate measure of infection history than clinical testing. The proportion of children with these antibodies (i.e., seroprevalence) can improve our understanding of SARS-CoV-2 by detecting more infections and eliminating potential bias due to local testing and reporting practices. Enhanced robustness of surveillance for respiratory infections in children, including records of mitigation policies in communities and schools, as well as seroprevalence data, would establish a better evidence base for policy decisions and response measures during future respiratory outbreaks.

Head blight is a serious fungal disease that results in yield loss and mycotoxin contamination in cereal crops worldwide. Head blight is primarily caused by members of the Fusarium graminearum species complex (FGSC), which consists of at least 16 species, including Fusarium asiaticum and Fusarium graminearum. FGSC members produce trichothecene mycotoxins and can be divided into four groups (chemotypes) depending on the type of trichothecenes produced: NIV (production of nivalenol), 3ADON (production of 3-acetyldeoxynivalenol), 15ADON (production of 15-acetyldeoxynivalenol), and NX-2 (production of 3α-acetoxy, 7α, 15-dihydroxy-12, and 13-epoxytrichothe-9-ene). Whereas F. graminearum mainly causes head blight in wheat-producing areas, F. asiaticum is associated primarily with rice head blight in eastern Asia. To date, the NCBI database archives 116 assembled genome sequences of F. graminearum, but only four accessions correspond to F. asiaticum. Accessions for three F. graminearum strains (PH-1 = NRRL 31084, CS3005, and FG-12) are chromosome assemblies (i.e., include contigs/scaffolds consisting of full-length or nearly full-length chromosomes), but none of the F. asiaticum accessions is a chromosome assembly. This announcement reports the assembled genome sequences of nine F. asiaticum strains and seven F. graminearum strains isolated from rice, wheat, barley, and other gramineous plants in Korea and China. [Figure: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Herbal medicines are widely used for clinical purposes worldwide. These herbs are susceptible to phytopathogenic fungal invasion during the culturing, harvesting, storage, and processing stages. The threat of fungal and mycotoxin contamination requires the evaluation of the health risks associated with these herbal medicines. In this study, we collected 138 samples of 23 commonly used herbs from 20 regions in China, from which we isolated a total of 200 phytopathogenic fungi. Through morphological observation and ITS sequencing, 173 fungal isolates were identified and classified into 24 genera, of which the predominant genera were Fusarium (27.74%) and Alternaria (20.81%), followed by Epicoccum (11.56%), Nigrospora (7.51%), and Trichocladium (6.84%). Quantitative analysis of the abundance of both Fusarium and Alternaria in herbal medicines via RT-qPCR revealed that the most abundant fungi were found on the herb Taraxacum mongolicum, reaching 300,000 copies/μL for Fusarium and 700 copies/μL for Alternaria. The in vitro mycotoxin productivities of the isolated Fusarium and Alternaria strains were evaluated by using liquid chromatography–tandem mass spectrometry (LC-MS/MS), and it was found that the Fusarium species mainly produced the acetyl forms of deoxynivalenol, while Alternaria species mainly produced altertoxins. These findings revealed widely distributed fungal contamination in herbal medicines and thus raise concerns for the sake of the quality and safety of herbal medicines.

Abstract Starch,as the main source of carbohydrates in the diet, provides energy for various activities of the body. Different types and structures of starch lead to variations in digestion and absorption, thereby affecting blood glucose levels and lipid metabolism in the body. Resistant starch (RS) has gained much attention because of its unique properties; it is not digested in the small intestine but ferments in the large intestine and produces short-chain fatty acids. RS has been found to play a crucial role in glucose homeostasis, fat metabolism, cholesterol levels and mineral absorption. Furthermore, RS has a high thermal stability, white color and low water holding capacity, making it useful in a wide range of food industry applications. This review aims to provide an update on the physiological effects of RS under physiological and pathological conditions, to provide information on the applications of RS in the food industry, and to assess whether dietary strategies to improve RS could have potential prevention and therapeutic effects for metabolic disorders associated with diabetes, obesity, and hyperlipidemia. Graphical Abstract

Highly r -process-enhanced (RPE) stars are rare and usually metal poor ([Fe/H] < −1.0), and they mainly populate the Milky Way halo and dwarf galaxies. This study presents the discovery of a relatively bright ( V = 12.72), highly RPE ( r -II) star ([Eu/Fe] = +1.32, [Ba/Eu] = −0.95), LAMOST J020623.21+494127.9. This star was selected from the Large Sky Area Multi-Object Fiber Spectroscopic Telescope medium-resolution ( R ∼ 7500) spectroscopic survey; follow-up high-resolution ( R ∼ 25,000) observations were conducted with the High Optical Resolution Spectrograph installed on the Gran Telescopio Canarias. The stellar parameters ( T _eff = 4130 K, $\mathrm{log}\,{\rm{g}}$ = 1.52, [Fe/H] = −0.54, ξ = 1.80 km s ^−1 ) have been inferred taking into account nonlocal thermodynamic equilibrium effects. The abundances of [Ce/Fe], [Pr/Fe], and [Nd/Fe] are +0.19, +0.65, and +0.64, respectively, relatively low compared to the Solar r -process pattern normalized to Eu. This star has a high metallicity ([Fe/H] = −0.54) compared to most other highly RPE stars and has the highest measured abundance ratio of Eu to H ([Eu/H] = +0.78). It is classified as a thin-disk star based on its kinematics and does not appear to belong to any known stream or dwarf galaxy.

Background: Presbycusis/Age-related hearing loss is a sensorineural hearing loss caused by age-related deterioration of the auditory system that poses a risk to the physical and mental health of older people, including social and cognitive decline. It is also associated with frailty, falls and depression. There are currently no specific medications for the treatment of presbycusis, and early detection and intervention are key to its prevention and management. Traditional Chinese medicine interventions may offer opportunities in the prevention and treatment of presbycusis, but there is no relevant review. Methods: Literature searches was conducted using PubMed, Cochrane Library, Web of Science, and China National Knowledge Infrastructure (CNKI) databases for review articles, research articles, clinical trials, meta-analyses, and case studies in animal models and clinical trials. Results: We summarized the pathological mechanisms associated with presbycusis, related to genetic factors, environment, lifestyle, and molecular mechanisms related to oxidative stress, mitochondrial dysfunction, and inflammatory pathways. It is suggested that traditional Chinese medicine interventions may offer opportunities in the prevention and treatment of presbycusis using active ingredients of herbs or formulas, acupuncture, and exercise such as Tai Chi Chuan or Ba Duan Jin. The active ingredients of herbs or formulas may exert ear protection through Nrf2-mediated antioxidant pathways, NF-kB and NLRP3-related anti-inflammatory signaling, and regulation of autophagy. Conclusions: Here, we review the pathogenetic factors and pathological mechanisms involved in presbycusis, as well as traditional Chinese medicine interventions and treatments, with the aim of providing a new perspective for the prevention and treatment of hearing loss in the elderly and further improving their quality of life.

Background: APOBEC family play an important role in cancer mutagenesis and tumor development. The role of APOBEC family in lung adenocarcinoma (LUAD) has not been studied comprehensively. Materials and methods: The expression data of pan-cancer as well as LUAD was obtained from public databases. The expression level of APOBEC family genes was analyzed in different normal and cancer tissues. APOBEC mutagenesis enrichment score (AMES) was utilized to evaluate the APOBEC-induced mutations and the relation of APOBEC with genomic instability. Gene set enrichment analysis was used to identify differentially enriched pathways. Univariate Cox regression and Lasso regression were applied to screen key prognostic genes. The immune cell infiltration was estimated by CIBERSORT. RT-qPCR assay, CCK-8 and Transwell assay were conducted to explore gene expression and lung cancer cell invasion. Results: Cancer tissues had significantly altered expression of APOBEC family genes and the expression patterns of APOBEC family were different in different cancer types. APOBEC3B was the most aberrantly expressed in most cancer types. In LUAD, we observed a significantly positive correlation of AMES with intratumor heterogeneity (ITH), tumor neoantigen burden (TNB), and tumor mutation burden (TMB). High AMES group had high mutation counts of DNA damage repair pathways, and high enrichment of cell cycle and DNA repair pathways. We identified four prognostic genes (LYPD3, ANLN, MUC5B, and FOSL1) based on AMES, and constructed an AMES-related gene signature. The expressions of four genes were enhanced and accelerated the invasion ability and viability of lung cancer cells. Furthermore, we found that high group increased oxidative stress level. Conclusions: APOBEC family was associated with genomic instability, DNA damage-related pathways, and cell cycle in LUAD. The AMES-related gene signature had a great potential to indicate the prognosis and guide immunotherapy/chemotherapy for patients suffering from LUAD.

Autophagy is the main intracellular degradation system by which cytoplasmic materials are transported to and degraded in the vacuole/lysosome of eukaryotic cells, and it also controls cellular differentiation and virulence in a variety of filamentous fungi. However, the contribution of the autophagic pathway to fungal development and pathogenicity in the important maize pathogen and mycotoxigenic fungus Fusarium verticillioides is still unknown. In this study, we characterized two autophagy-related proteins, FvAtg4 and FvAtg8. The F. verticillioides deletion mutants ΔFvAtg4 and ΔFvAtg8 were impaired in autophagosome formation, aerial hyphal formation, sexual growth, lipid turnover, pigmentation and fungal virulence. Interestingly, ΔFvAtg4 and ΔFvAtg8 were defective in fumonisin B1 (FB1) synthesis, which may have resulted from decreased intracellular levels of alanine in the mutants. Our results indicate that FvAtg4 and FvAtg8 contribute to F. verticillioides pathogenicity by regulating the autophagic pathway to control lipid turnover, fumonisin biosynthesis, and pigmentation during its infectious cycle.

Henoch-Schönlein purpura nephritis (HSPN) is a common vasculitis that mostly affects children, and previous studies have indicated that genetic factors may influence disease susceptibility. The aim of this study was to evaluate a possible association of three interleukin-2 (IL-2) gene polymorphisms (rs3136534, rs2069776, and rs2069762) with HSPN in the Chinese population. A total of 81 patients with HSPN and 200 healthy children were enrolled. The distribution of genotypes, allelic frequencies, and haplotype frequencies among the three IL-2 polymorphisms were analyzed using the Sequenom MassARRAY system by means of matrix-assisted laser desorption ionization-time of flight mass spectrometry method. Compared to the healthy controls, genotyping analysis demonstrated rs3136534 was associated with a decreased HSPN risk in the dominant inheritance model (G/T+T/T vs. G/G; OR, 0.54; 95% CI, 0.31–0.93). However, the frequency of the T allele and haplotypes of rs3136534 showed no statistical significance. For the frequency of genotype, allele, and haplotype of the rs2069776 and rs20697622 polymorphisms, no significant differences were observed between HSPN patients and controls. Our results suggest that the rs3136534 polymorphism of the IL-2 gene is associated with susceptibility to HSPN in Chinese children. Keywords: ; ;

BackgroundAtopic dermatitis (AD) is a common chronic inflammatory skin disease, adversely affecting nearly 20% of the pediatric population worldwide. Interleukin-4 (IL-4) and interleukin-18 (IL-18) are considered to be involved in the pathogenesis and development of AD. The aim of this study was to investigate the association of IL-4 and IL-18 gene polymorphisms with the susceptibility and severity of AD in Chinese children.MethodsSix candidate single nucleotide polymorphisms (SNPs) in IL-4 and IL-18 genes were genotyped through multi-PCR combined with next-generation sequencing in 132 AD children and 100 healthy controls, and all the analyses were performed on blood genome DNA.ResultsThe frequencies of G allele, CG genotype and CG + GG genotype of IL-4 rs2243283, as well as the haplotype IL-4/GTT (rs2243283-rs2243250-rs2243248) were all significantly decreased in AD patients compared with the controls [G vs. C: P = 0.033, OR = 0.59; CG vs. CC: P = 0.024, OR = 0.47; CG + GG vs. CC: P = 0.012, OR = 0.49; GTT vs. CCT: P = 0.011, OR = 0.65]. Moreover, the frequencies of A allele, AA genotype and AG + AA genotype of IL-18 rs7106524, along with the haplotype IL-18/CAA (rs187238-rs360718-rs7106524) were statistically increased in the severe AD patients (A vs. G: P

Accurate determination of the stellar atmospheric parameters of RR Lyrae stars (RRLs) requires short individual exposures of the spectra to mitigate pulsation effects. We present improved template-matching methods to determine the stellar atmospheric parameters of RRLs from single-epoch spectra of the Large Sky Area Multi-Object Fiber Spectroscopic Telescope, also known as the Guoshoujing Telescope (LAMOST). We determine the radial velocities and stellar atmospheric parameters (effective temperature T _eff , surface gravity $\mathrm{log}\,g$ , and metallicity [M/H]) of 10,486 and 1027 RRLs from 42,729 LAMOST low-resolution spectra (LRS) and 7064 LAMOST medium-resolution spectra (MRS), respectively. Our results are in good agreement with the parameters of other databases, where the external uncertainties of T _eff , $\mathrm{log}\,g$ , and [M/H] for LRS/MRS are estimated to be 207/142 K, 0.21/0.16 dex, and 0.24/0.18 dex, respectively. We conclude with the variation characteristics of the radial velocities (RV) and stellar atmospheric parameters for RRLs during the pulsation phase. There is a significant difference of 28 ± 21 km s ^−1 between the peak-to-peak amplitude ( A _ptp ) of RV from the H α line (RV _H _α ) and from metal lines (RV _metal ) for RRab, whereas it is only 4 ± 17 km s ^−1 for RRc. The A _ptp of T _eff is 930 ± 456 and 409 ± 375 K for RRab and RRc, respectively. The $\mathrm{log}\,g$ of RRab shows a mild variation of approximately 0.22 ± 0.42 dex near the phase of φ = 0.9, while that of RRc almost remains constant. The [M/H] of RRab and RRc show a minor variation of about 0.25 ± 0.50 and 0.28 ± 0.55 dex, respectively, near the phase of φ = 0.9. We expect that the determined stellar atmospheric parameters would shed new light on the study of stellar evolution and pulsation, the structure of the Milky Way, as well as other research fields.

Very metal-poor (VMP) stars record the signatures of early accreted galaxies, making them essential tools for unraveling the early stages of Galaxy formation. Understanding the origin of VMP stars requires comprehensive studies of their chemical compositions and kinematics, which are currently lacking. Hence, we conduct a chemodynamical analysis of 352 VMP stars selected from one of the largest uniform high-resolution VMP star samples, jointly obtained from LAMOST and Subaru. We apply a friends-of-friends clustering algorithm to the master catalog of this high-resolution sample, which consists of 5778 VMP stars. It results in 131 dynamically tagged groups with 89 associated with known substructures in the Milky Way, including Gaia-Sausage-Enceladus (GSE), Thamnos, Helmi streams, Sequoia, Wukong, Pontus, and the very metal-poor disk (VMPD). Our findings are: (i) the VMPD shows lower Zn abundances than the rest, which indicates that it could be a relic of small stellar systems; (ii) Sequoia shows moderately high r -process abundances; (iii) Helmi streams show deficiencies in carbon and light neutron-capture elements; (iv) the fraction of carbon-enhanced metal-poor stars with no enhancement in heavy elements (CEMP-no stars) seems low in the VMPD and the Helmi streams; and (v) a subgroup in GSE exhibits a very high fraction of r -process enhanced stars, with four out of five showing [Eu/Fe]> +1.0. The abundance patterns of other elements in VMP substructures largely match the whole VMP sample. We also study large-scale correlations between abundance ratios and kinematics without classifying stars into substructures, but it does not yield significant correlations once the overall chemical evolution is considered for most elements.

Carbon stars are excellent kinematic tracers of galaxies and play important roles in understanding the evolution of the Galaxy. Therefore, it is worthwhile to search for them in a large number of spectra. In this work, we build a new carbon star catalog based on the Large Sky Area Multi-Object Fiber Spectroscopy Telescope (LAMOST) DR7 spectra. The catalog contains 4542 spectra of 3546 carbon stars, identified through line index and near-infrared color–color diagrams. Through visual inspection of the spectra, we further subclassify them into 925 C–H, 384 C–R, 608 C–N, and 1292 Ba stars. However, 437 stars could not be subclassified due to their low signal-to-noise. Moreover, by comparing with the LAMOST DR7 pipeline we find 567 more carbon stars and visually subclassify them. We find that on the J − H versus H − K _s two-color diagram, C–N stars can be reliably distinguished from the other three subtypes. Additionally, by utilizing the Gaia distance, we study the distribution of carbon stars in the H-R diagram and identify 258 dwarf carbon stars by the criterion M _G > 5.0 mag. Finally, we present the spatial distribution in Galactic coordinates of the 3546 carbon stars. The majority of C–N, C–R, and Ba stars are distributed at low Galactic latitudes, while most C–H and dwarf carbon stars are distributed at high Galactic latitudes.

Utilizing astrometric parameters sourced from Gaia Data Release 3 and radial velocities obtained from various spectroscopic surveys, we identify 519 high-velocity stars (HiVels) with a total velocity in the Galactocentric rest frame greater than 70% of their local escape velocity under the Gala MilkyWayPotential . Our analysis reveals that the majority of these HiVels are metal-poor late-type giants, and we show nine HiVels that are unbound candidates to the Galaxy with escape probabilities of 50%. To investigate the origins of these HiVels, we classify them into four categories and consider the impact of the Large Magellanic Cloud (LMC) potential on their backward-integration trajectories. Specifically, we find that one of the HiVels can track back to the Galactic center, and three HiVels may originate from the Sagittarius dwarf spheroidal galaxy (Sgr dSph). Furthermore, some HiVels appear to be ejected from the Galactic disk, while others formed within the Milky Way or have an extragalactic origin. Given that the LMC has a significant impact on the orbits of Sgr dSph, we examine the reported HiVels that originate from the Sgr dSph, with a few of them passing within the half-light radius of the Sgr dSph.

IntroductionAtopic Dermatitis (AD) is a chronic inflammatory skin disease that affects almost 20% of children and 2 -10% of adults worldwide. Previous studies revealed that Interleukin-6 (IL-6) plays an essential role in autoimmune and chronic inflammatory diseases. This study aims to investigate the associations between IL-6 polymorphisms and AD.MethodsBlood samples were collected from 132 AD patients and 100 controls, and single nucleotide polymorphisms (SNPs) in IL-6 (rs2069840 (C/G), rs2066992 (G/T), rs2069837 (A/G) and rs1800796 (G/C)) were analyzed using Multiplex PCR-Based Next Generation Sequencing (NGS).ResultsResults showed that the A/G genotype of IL-6/rs2069837 was significantly associated with a 1.933-fold increased risk of AD compared to those patients with A/A genotype (OR 1.933; 95%CI 1.086-3.438; p=0.024). The combined A/G-G/G genotype raised AD risk by 1.856 times compared to patients with the A/A genotype in dominant model (OR: 1.856; 95% CI: 1.056-3.261; p=0.030). No association was observed for 3 other SNPs and 4 haplotypes.DiscussionThese findings suggested that the A/G genotype of IL-6/rs2069837 was more susceptible to AD than A/A genotype in Chinese Han children, indicating the risk role of IL-6/rs2069837 in the occurrence of AD.

In order to derive sodium abundances and investigate the effects of non-local thermodynamic equilibrium (NLTE) on the formation of H-band Na I lines, we update the sodium atomic model by incorporating collision rates with hydrogen from new quantum-mechanical calculations. The differential Na abundances for 13 sample stars are obtained by analyzing high-resolution H-band spectra from the Apache Point Observatory Galactic Evolution Experiment (APOGEE) and optical spectra under both local thermodynamic equilibrium (LTE) and NLTE conditions. Consistent abundances from both bands suggest that our updated atomic model is valid for studying the formation of H-band Na I lines. Our calculations show that, in our stellar parameter space, NLTE effects are negative and can result in corrections larger than −0.4 dex on optical lines. The corrections on H-band Na I lines are typically small, within about 0.05 dex, but not negligible if accurate sodium abundance is desired. We note that the [Na/Fe] ratios favor the theoretical galactic chemical model.

Members of the Fusarium graminearum species complex (FGSC) cause extensive yield losses in cereal production worldwide, and food safety concerns due to the accumulation of Fusarium toxins in infected grains. Among these pathogens, F. meridionale is responsible for Fusarium head blight of wheat and rice, ear and stalk rot of maize, and pod blight of soybean. Here, we present an improved genome assembly of F. meridionale strain SR5 isolated from rice in China based on PacBio long-read sequencing and Illumina short-read sequencing technology. The assembled genome of SR5 has a total size of 36.82 Mb, an N50 scaffold length of 7.82 Mb, nine scaffolds, and encodes 12,409 predicted genes. These high-quality data expand FGSC genomic resources and provide a valuable resource for better understanding their genetic diversity and the molecular basis of pathogenesis, which will facilitate the development of an effective control strategy.[Graphic: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Based on the proper motions and radial velocities from Gaia Data Release 3, we identify two hypervelocity stars (HVSs) that may originate from the Galactic center (GC). We select the candidates by first filtering for all Gaia stars with Galactocentric radial velocities >500 km s ^−1 . We also require the candidates cross the Galactic midplane only once at 80% confidence, as determined by backwards orbit integration in several models of the Galactic potential, given the uncertainties in the Gaia measurables. The final two HVS candidates are the only such stars in our sample whose backwards-integrated trajectories pass within 1 kpc of the GC, suggesting a potential GC origin. We discuss possible ejection scenarios for these HVSs, in particular, by finding that ejection via the Hills mechanism is unlikely unless the HVS trajectories were significantly altered by local or large-scale perturbations to the assumed Galactic potential, e.g., the Large Magellanic Cloud. Interestingly, one of the HVSs ejects in a direction that is curiously aligned with the clockwise stellar disk around Sgr A*, suggesting a possible connection. We also discuss that the two stars may be ejected by other mechanisms.

We report the discovery of a dark companion to 2MASS J15274848+3536572 with an orbital period of 6.14 hr. Combining the radial velocity from LAMOST observations and modeling of the multiband light curve, one obtains a mass function of ≃0.131 M _⊙ , an inclination of $45\buildrel{\circ}\over{.} {20}_{-0\buildrel{\circ}\over{.} \,20}^{+0\buildrel{\circ}\over{.} \,13}$ , and a mass ratio of ${0.631}_{-0.003}^{+0.014}$ , which demonstrate the binary nature of the dark companion with a mass of 0.98 ± 0.03 M _⊙ and a main-sequence K9-M0 star of 0.62 ± 0.01 M _⊙ . LAMOST optical spectra at a range of orbital phases reveal extra-peaked H _α emission that suggests the presence of an accretion disk. The dark companion does not seem to be a white dwarf because of the lack of any observed dwarf nova outbursts in the long-term data archive, although a magnetic white dwarf cannot be excluded. Alternatively, we propose a scenario wherein the dark companion is a neutron star, but we have not detected radio pulsations or a single pulse from the system with the FAST (Five-hundred-meter Aperture Spherical radio Telescope), which hints at a radio-quiet compact object. If the dark companion is identified as a neutron star, it will be the nearest (∼118 pc) and lightest neutron star. Furthermore, kinematic analysis of the system’s orbit in the galaxy may suggest its supernova event is associated with the radionuclide ^60 Fe signal observed from deep-sea crusts. This radio-quiet and X-ray-dim nearby neutron star may resemble an XDINS (X-ray-dim isolated neutron star) but in a binary.

ABSTRACT: Objectives: Lipopeptides have been revealed as good potential biocontrol agents against various pathogenic microbes. In the present work, we report the draft genome sequence of a lipopeptide-producing strain of Bacillus amyloliquefaciens (7D3) that showed good antifungal activity against the azole-resistant pathogenic fungus Fusarium graminearum. Methods: Whole-genome sequencing of strain 7D3 was performed on an Illumina MiSeq 300 platform. Raw data were cleaned using Trim Galore v.0.4.0 and were checked for quality using FastQC. De novo assembly was performed using the SOAPdenovo2 package. Genes responsible for the biosynthesis of secondary metabolites were identified using antiSMASH. Results: Bacillus amyloliquefaciens 7D3 genome assembly resulted in a total genome size of 3 913 220 bp with a G+C content of 46.13%. There were 3998 predicted genes with 72 tRNAs and 9 rRNAs. A total of ten gene clusters were found to be related to secondary metabolite biosynthesis, of which five were identified as lipopeptide synthesis clusters. Conclusion: This study presents the genome sequence of B. amyloliquefaciens 7D3, which exhibited intense antagonistic activity against azole-resistant fungi. The whole genome sequence will help in the search for novel antifungal peptides against drug-resistant pathogens.

Abstract Production of the Fusarium toxin deoxynivalenol (DON) is associated with oxidative stress and has been indicated to be part of an adaptive response to oxidative stress in the important wheat fungus Fusarium graminearum. In this study, we found that the antioxidant methyl gallate (MG) displays inhibitory effects against mycelial growth, conidial formation and germination, and DON biosynthesis in F. graminearum in a dose-dependent manner. Treatment with 0.05% (w/v) MG resulted in an abnormal swollen conidial morphology. The expression of the TRI genes involved in DON biosynthesis was significantly reduced, and the induction of Tri1-GFP green fluorescence signals in the spherical and crescent-shaped toxisomes was abolished in the MG-treated mycelium. RNA-Seq analysis of MG-treated F. graminearum showed that 0.5% (w/v) MG inhibited DON production by possibly altering membrane functions and oxidoreductase activities. Coupled with the observations that MG treatment decreases catalase, POD and SOD activity in F. graminearum. The results of this study indicated that MG displays antifungal activity against DON production by modulating its oxidative response. Taken together, the current study revealed the potential of MG in inhibiting mycotoxins in F. graminearum. Graphical abstract

Fusarium fujikuroi species complex (FFSC) strains are a major concern for food quantity and quality due to their strong ability to synthesize mycotoxins. The effects of interacting conditions of water activity, temperature, and incubation time on the growth rate, toxin production, and expression level of biosynthetic genes were examined. High temperature and water availability increased fungal growth. Higher water activity was in favor of toxin accumulation. The maximum amounts of fusaric acid (FA) and fumonisin B1 (FB1) were usually observed at 20–25 °C. F. andiyazi could produce a higher content of moniliformin (MON) in the cool environment than F. fujikuroi. The expression profile of biosynthetic genes under environmental conditions varied wildly; it was suggested that these genes might be expressed in a strain-dependent manner. FB1 concentration was positively related to the expression of FUM1, while a similar correlation of FUB8 and FUB12 with FA production could be observed in F. andiyazi, F. fujikuroi, and F. subglutinans. This study provides useful information in the monitoring and prevention of such toxins entering the maize production chain.

An antibiotic produced by Paenibacillus polymyxa 7F1 was studied. The 7F1 strain was isolated from the rhizosphere of a wheat field. Response surface methodology was used to optimize the physicochemical parameters. The strain showed broad-spectrum activity against several plant pathogens. Identification of the strain was realized based on 16s rRNA gene and gyrB gene sequencing. The antibiotic was optimized by one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The suitable antibiotic production conditions were optimized using the one-factor-at-a-time method. The individual and interaction effects of three independent variables: culture temperature, initial pH, and culture time, were optimized by Box-Behnken design. The 16SrRNA gene sequence (1239 nucleotides) and gyrB gene (1111 nucleotides) were determined for strain 7F1 and shared the highest identities to those of Paenibacillus polymyxa. The results showed the optimal fermentation conditions for antibiotics produced by Paenibacillus polymyxa 7F1 were a culture temperature of 38 °C, initial pH of 8.0, and culture time of 8 h. The antibiotics produced by Paenibacillus polymyxa 7F1 include lipopeptides such as iturin A and surfactin. The results provide a theoretical basis for the development of bacteriostatic biological agents and the control of mycotoxins.

Abstract Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. Graphical abstract

Abstract Pyruvate dehydrogenase kinase (PDK) is a mitochondrial enzyme in a variety of eukaryotes, including the plant pathogen Fusarium graminearum. This enzyme can reduce the oxidation of glucose to acetyl-coA by phosphorylation and selectively inhibits the activity of pyruvate dehydrogenase (PDH), which is a kind of pyruvate dehydrogenase complex (PDC). In this study, we investigated the F. graminearum pyruvate dehydrogenase kinase encoded by FgPDK2, which is a homologue of Neurospora crassa PDK2. The disruption of the FgPDK2 gene led to several phenotypic defects including effects on mycelial growth, conidiation, pigmentation, and pathogenicity. The mutants also showed decreased resistance to osmotic stress and cell membrane/wall-damaging agents. The FgPDK2 deletion mutant exhibited reduced virulence. All of these defects were restored by genetic complementation of the mutant with the complete FgPDK2 gene. Overall, the results demonstrated that FgPDK2 is crucial for the growth of F. graminearum and can be exploited as a potential molecular target for novel fungicides to control Fusarium head blight caused by F. graminearum. Graphical abstract

The nonlocal thermodynamical equilibrium (NLTE) line formation of Y i and Y ii is considered in 1D LTE model atmospheres of FGK-type stars. The model atom was constructed with the most up-to-date atomic data, including quantum cross sections and rate coefficients for transitions in inelastic collisions of Y i and Y ii with hydrogen atoms. For seven reference stars, we obtained an agreement between NLTE abundances inferred from the two ionization stages, while the difference in LTE abundance (Y i and Y ii ) can reach up to −0.31 dex. In the atmospheres of FGK-type stars, for both Y i and Y ii lines, the NLTE abundance corrections are positive. In solar metallicity stars, the NLTE abundance corrections for Y ii lines do not exceed 0.12 dex, while in atmospheres of metal-poor stars, they do not exceed 0.21 dex. For Y i lines, the NLTE abundance corrections can reach up to ∼0.5 dex. We determined the yttrium NLTE abundances for a sample of 65 F and G dwarfs and subgiants in the −2.62 ≤ [Fe/H] ≤ +0.24 metallicity range, using high-resolution spectra. For stars with [Fe/H] ≤ −1.5, [Y/Fe] versus [Fe/H] diagram reveals a positive trend with an average value of [Y/Fe] ≃ 0. For metal-poor stars, among Sr, Y, and Zr, the arrangement [Sr/Fe] < [Y/Fe] < [Zr/Fe] remains consistent. The current study is useful for Galactic chemical evolution research. The model atom will be applied for NLTE yttrium abundance determination in very metal-poor stars studied with LAMOST and Subaru.

A small fraction of giants possess photospheric lithium (Li) abundance higher than the value predicted by the standard stellar evolution models, and the detailed mechanisms of Li enhancement are complicated and lack a definite conclusion. In order to better understand the Li enhancement behaviors, a large and homogeneous Li-rich giant sample is needed. In this study, we designed a modified convolutional neural network model called Coord-DenseNet to determine the A (Li) of Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) low-resolution survey (LRS) giant spectra. The precision is good on the test set: MAE = 0.15 dex, and σ = 0.21 dex. We used this model to predict the Li abundance of more than 900,000 LAMOST DR8 LRS giant spectra and identified 7768 Li-rich giants with Li abundances ranging from 2.0 to 5.4 dex, accounting for about 1.02% of all giants. We compared the Li abundance estimated by our work with those derived from high-resolution spectra. We found that the consistency was good if the overall deviation of 0.27 dex between them was not considered. The analysis shows that the difference is mainly due to the high A (Li) from the medium-resolution spectra in the training set. This sample of Li-rich giants dramatically expands the existing sample size of Li-rich giants and provides us with more samples to further study the formation and evolution of Li-rich giants.

The main component of creosote obtained from dry wood distillation—guaiacol—is a natural antioxidant that has been widely used in pharmaceutical and food preservation applications. However, the antifungal mechanism of guaiacol against phytopathogens remains unclear. In this study, we found that guaiacol exerts inhibitory effects against mycelial growth, conidial formation and germination, and deoxynivalenol (DON) biosynthesis in Fusarium graminearum in a dose-dependent manner. The median effective concentration (EC50) value of guaiacol for the standard F. graminearum strain PH-1 was 1.838 mM. Guaiacol strongly inhibited conidial production and germination. The antifungal effects of guaiacol may be attributed to its capability to cause damage to the cell membrane by disrupting Ca2+ transport channels. In addition, the decreased malondialdehyde (MDA) levels and catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activity by guaiacol treatment indicate that guaiacol displays activity against DON production by modulating the oxidative response in F. graminearum. Taken together, this study revealed the potentials of antioxidant in inhibiting mycotoxins in F. graminearum.

Background: The traditional Chinese medicine formula ErLong ZuoCi (ELZC) has been extensively used to treat age-related hearing loss (ARHL) in clinical practice in China for centuries. However, the underlying molecular mechanisms are still poorly understood.Objective: Combine network pharmacology with experimental validation to explore the potential molecular mechanisms underlying ELZC with a systematic viewpoint.Methods: The chemical components of ELZC were collected from the Traditional Chinese Medicine System Pharmacology database, and their possible target proteins were predicted using the SwissTargetPrediction database. The putative ARHL-related target proteins were identified from the database: GeneCards and OMIM. We constructed the drug-target network as well as drug-disease specific protein-protein interaction networks and performed clustering and topological property analyses. Functional annotation and signaling pathways were performed by gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. Finally, in vitro experiments were also performed to validate ELZC’s key target proteins and treatment effects on ARHL.Results: In total, 63 chemical compounds from ELZC and 365 putative ARHL-related targets were identified, and 1860 ARHL-related targets were collected from the OMIM and GeneCards. A total of 145 shared targets of ELZC and ARHL were acquired by Venn diagram analysis. Functional enrichment analysis suggested that ELZC might exert its pharmacological effects in multiple biological processes, such as cell proliferation, apoptosis, inflammatory response, and synaptic connections, and the potential targets might be associated with AKT, ERK, and STAT3, as well as other proteins. In vitro experiments revealed that ELZC pretreatment could decrease senescence-associated β-galactosidase activity in hydrogen peroxide-induced auditory hair cells, eliminate DNA damage, and reduce cellular senescence protein p21 and p53. Finally, Western blot analysis confirmed that ELZC could upregulate the predicted target ERK phosphorylation.Conclusion: We provide an integrative network pharmacology approach, in combination with in vitro experiments to explore the underlying molecular mechanisms governing ELZC treatment of ARHL. The protective effects of ELZC against ARHL were predicted to be associated with cellular senescence, inflammatory response, and synaptic connections which might be linked to various pathways such as JNK/STAT3 and ERK cascade signaling pathways. As a prosperous possibility, our experimental data suggest phosphorylation ERK is essential for ELZC to prevent degeneration of cochlear.

Based on the spectra with high resolution and a high signal-to-noise ratio, we investigate the enrichment history of the s-process element in seven barium (Ba) stars by measuring their Ba odd isotope fraction. It is found that the relative contributions of the s-process to their Ba abundance are 91.4±25.7%, 91.4±34.3%, 82.9±28.5%, 77.1±31.4%, and 71.4±37.1% for REJ 0702+129, HD 13611, BD+80°670, HR 5692, and HD 202109, respectively. Our results suggest that these five Ba stars have a prominent s-process signature, which indicates that their heavy elements mainly come from their former AGB companions (now WDs) by mass transfer, while the r-process contribution can naturally be explained by the evolution of the Milky Way. The s-process contribution of BD+80°670 is 51.4±31.4%, which is the lowest among our seven sample stars. Considering its lower values of both [Ba/Nd] and [Ba/Eu], we suspect that BD+68°1027 is likely to be a r-rich Ba star and has similar origins to the CEMP-r/s stars. HD 218356 has an unreasonable s-process contribution over 100%. Combining its stellar atmospheric parameters and the evolutionary stage, we speculate that HD 218356 is a more evolved extrinsic Ba star, and its massive companion should have the largest s-process efficiency in our samples.

Abstract Fusarium mycotoxin contamination of both foods and feeds is an inevitable phenomenon worldwide. Deoxynivalenol, nivalenol, zearalenone, T-2 toxin and fumonisin B1 are the most studied Fusarium mycotoxins. Co-contamination of mycotoxins has also been studied frequently. Fusarium mycotoxins occur frequently in foods at very low concentrations, so there is a need to provide sensitive and reliable methods for their early detection. The present review provides insight on the types, toxicology and occurrence of Fusarium mycotoxins. It further elucidates various detection methods of mycotoxin production from Fusarium strains, with a special focus on chromatographic and immunochemical techniques.

Zearalenone (ZEN) is produced by Fusarium spp. and is widely found in moldy wheat, corn, and other grains. ZEN has a strong toxicity and causes reproductive and immune disorders and estrogenic syndrome in animals and humans. Biodegradation has been demonstrated as an efficient way to control the hazardous effect of ZEN. A promising way to apply biodegradation in feed is to introduce anaerobic ZEN-degrading microorganisms, which can function during the digestion process in animal intestines. The aim of this study was to isolate anaerobic ZEN-degrading bacteria from anaerobic environments. A strain named F39 was isolated from animal intestinal contents and had a ZEN-degradation rate of 87.35% in 48 h to form trace amount of α- and β-zearalenol. Based on the morphological and physiological properties and phylogenetic analysis of 16S rRNA and rpoB gene sequences, F39 was identified as Clostridium sporogenes. The optimum temperature for the growth of F39 was 37 °C, the optimum pH was 7.0, and the most suitable carbon source was beef extract, while the optimal conditions for the degradation of ZEN were as follows: 35 °C, pH 7.0, and GAM medium. ZEN was degraded by F39 with a high efficiency in the concentration range of 1–15 mg/L. The bioactive factors responsible for ZEN degradation were mainly distributed intracellularly. F39 can degrade most of the ZEN present, but a small amount is broken down into two secondary metabolites, α- and β-zearalenol, and the toxicity of the degradation products is reduced. With an efficiency of 49%, F39 can more effectively degrade ZEN in wheat-based feedstuffs than in other feedstuff, and the degradation efficiency was pH related. To the best of our knowledge, this is the first report of Clostridium sporogenes F39’s ability to maintain the biodegradation potentials.

All organisms must secure essential trace elements (e.g., Cu) for survival and reproduction. However, excess trace element accumulation in cells is highly toxic. The maintenance of copper (Cu) homeostasis has been extensively studied in mammals, bacteria, and yeast but not in plant pathogens. In this study, we investigated the molecular mechanisms of copper tolerance in Fusarium graminearum, the important wheat head scab fungus. RNA-seq revealed induced expression of the P-type ATPase transporter FgCrpA and metallothionein (MT) FgCrdA after excess Cu treatment. Deletion of FgCrpA but not FgCrdA resulted in reduced tolerance to Cu toxicity. The “Cu fist” transcription factor FgAceA was involved in Cu detoxification through activation of FgCrpA. △FgAceA was more sensitive to copper toxicity than △FgCrpA and overexpression of FgCrpA restored copper tolerance in △FgAceA. FgAceA negatively regulated aurofusarin production and its biosynthetic gene expression. △FgCrpA and △FgAceA were reduced in virulence in flowering wheat heads and synthesized decreased amounts of the mycotoxin deoxynivalenol when challenged with excess Cu. Taken together, these results suggest that mediation of Cu tolerance in F. graminearum mainly relies on the Cu efflux pump and that FgAceA governs Cu detoxification through activation of FgCrpA.

Deoxynivalenol (DON), a common mycotoxin of type B trichothecene, is produced mainly by several Fusarium species. DON causes great losses in farming and poses severe safety risks to human and animal health. Thus, DON contamination in cereals and DON toxicity are of worldwide concern. In this study, we screened the bacterial consortium C20, which efficiently degraded almost 70 μg ml−1 DON within 5 days. The bacterial consortium also had the ability to degrade 15-acetyl-DON, 3-acetyl-DON, and T-2 toxin. The bacterial consortium C20 was able to degrade DON under a wide range of pH and temperature conditions. The optimal temperature and pH for DON degradation were 30°C and pH 8.0, respectively. The bacterial consortium C20 comprised of different bacterial genera, and several strains were found to significantly increase when cultured in Mineral Medium with 100 μg ml−1 DON based on the analysis of the sequences of the hypervariable V3-V4 region of the 16S rRNA gene. 3-keto-DON was confirmed as a degradation product of DON by liquid chromatography/time-of-flight/mass spectrometry (LC-TOF-MS) and nuclear magnetic resonance (NMR) analyses. The results indicated that the bacterial consortium C20 is a potential candidate for the biodegradation of DON in a safe and environmentally friendly manner.

Fusarium graminearum and Fusarium verticillioides are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies for controlling these diseases have been developed. However, due to a lack of resistant cultivars and the hazards of chemical fungicides, efforts are now focused on the biocontrol of plant diseases, which is a more sustainable and environmentally friendly approach. In the present study, the lipopeptide mycosubtilin purified from Bacillus subtilis ATCC6633 significantly suppressed the growth of F. graminearum PH-1 and F. verticillioides 7600 in vitro. Mycosubtilin caused the destruction and deformation of plasma membranes and cell walls in F. graminearum hyphae. Additionally, mycosubtilin inhibited conidial spore formation and germination of both fungi in a dose-dependent manner. In planta experiments demonstrated the ability of mycosubtilin to control the adverse effects caused by F. graminearum and F. verticillioides on wheat heads and maize kernels, respectively. Mycosubtilin significantly decreased the production of deoxynivalenol (DON) and B-series fumonisins (FB1, FB2 and FB3) in infected grains, with inhibition rates of 48.92, 48.48, 52.42, and 59.44%, respectively. The qRT-PCR analysis showed that mycosubtilin significantly downregulated genes involved in mycotoxin biosynthesis. In conclusion, mycosubtilin produced by B. subtilis ATCC6633 was shown to have potential as a biological agent to control plant diseases and Fusarium toxin contamination caused by F. graminearum and F. verticillioides.

Superflares are large explosive events on stellar surfaces, much larger than solar flares, but it remains unclear whether they share the same origin. Here, the authors analysed 48 superflare stars and determine the relation between their chromospheric activity and the occurrence of superflares.