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13 results on '"Jin, Guoen"'

2. Long-term ventilatory adaptation and ventilatory response to hypoxia in plateau pika (Ochotona curzoniae): role of nNOS and dopamine

Author

Pichon, Aurelien, Zhenzhong, Bai, Favret, Fabrice, Jin, Guoen, Shufeng, Han, Marchant, Dominique, Richalet, Jean-Paul, and Ge, Ri-Li

Subjects
Hypoxia -- Care and treatment, Hypoxia -- Research, Dopamine -- Physiological aspects, Dopamine -- Research, Artificial respiration -- Usage, Biological sciences
Abstract

We assessed ventilatory patterns and ventilatory responses to hypoxia (HVR) in high-altitude (HA) plateau pikas, repetitively exposed to hypoxic burrows, and control rats. We evaluated the role of neuronal nitric oxide synthase (nNOS) and dopamine by using S-methyl-L-thiocitrulline (SMTC) inhibitor and haloperidol antagonist, respectively. Ventilation ([V.sub.I]) was measured using a whole body plethysmograph in conscious pikas (n = 9) and low-altitude (LA) rats (n = 7) at different [MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII] (56, 80, 111,150, and 186 mmHg) and in HA acclimatized rats (n = 9, 8 days at 4,600 m) at two different [MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII] (56 and 80 mmHg). The effects of NaCl, SMTC, and haloperidol on ventilatory patterns were assessed in pikas at [MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII] = 56 and 80 mmHg. We observed a main species effect with larger [V.sub.I], tidal volume (VT), inspiratory time/total time ([T.sub.i]/[T.sub.tot]), and a lower expiratory time in pikas than in LA rats. Pikas had also a larger VT and lower respiratory frequency compared with HA rats in hypoxia. HVR of pikas and rats were not statistically different. In pikas, SMTC induced a significant increase in [V.sub.I] and VT for a [MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII] of 56 mmHg, but had no effect for a [P.sub.I][O.sub.2] of 80 mmHg, i.e., the living altitude of pikas. In pikas, haloperidol injection had no effect on any ventilatory parameter. Long-term ventilatory adaptation in pikas is mainly due to an improvement in respiratory pattern (VT and [T.sub.i]/[T.sub.tot]) with no significant improvement in HVR. The sensitivity to severe acute hypoxia in pikas seems to be regulated by a peripheral nNOS mechanism. adaptation; control of breathing; dopamine doi: 10.1152/ajpregu.00108.2009.

Published
2009

6. Molecular cloning and characterization of hemoglobin α and β chains from plateau pika (Ochotona curzoniae) living at high altitude

Author

Cao Yue, Ma Lan, Yang Yingzhong, Jin Guoen, Yun Haixia, Bai Zhenzhong, and Ge Rili

Subjects
DNA, Complementary, Phenylalanine, Ochotona curzoniae, Molecular Sequence Data, Glycine, Codon, Initiator, Zoology, Molecular cloning, Polymerase Chain Reaction, Conserved sequence, Hemoglobins, Phylogenetics, Serine, Genetics, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Pika, Conserved Sequence, Phylogeny, DNA Primers, chemistry.chemical_classification, Base Sequence, biology, Ecology, Altitude, Hemoglobin A, Valine, Lagomorpha, General Medicine, Effects of high altitude on humans, biology.organism_classification, Adaptation, Physiological, Amino acid, Amino Acid Substitution, chemistry, Codon, Terminator, Hemoglobin, Asparagine
Abstract

Hemoglobin (Hb) plays an important role in oxygen transfer from lung to tissues. Possession of a Hb with high oxygen affinity helps highland animals to adapt to high altitude, has been studied profoundly. Plateau pika (Ochotona curzoniae), a native species living at 3,000-5,000 m above sea level on Qinghai-Tibet Plateau, is a typical hypoxia and low temperature tolerant mammal. To investigate the possible mechanisms of plateau pika Hb in adaptation to high altitude, the complete cDNA and amino acid sequences of plateau pika hemoglobin alpha and beta chains have been described. Compared with human Hb, alterations in important regions can be noted: alpha111 Ala-->Asn, beta35 Tyr-->Phe, beta112 Cys-->Val, beta115 Ala-->Ser, and beta125 Pro-->Gln. Phylogenetic analysis of alpha and beta chains shows that plateau pika is closer to rabbit than to other species. This study provides essential information for elucidating the possible roles of hemoglobin in adaptation to extremely high altitude in plateau pika.

Published
2007

10. Molecular cloning of hemoglobin alpha-chain gene from Pantholops hodgsonii, a hypoxic tolerance species

Author

Bai Zhenzhong, Yang Yingzhong, Cao Yue, Ge Rili, Ma Lan, Yunden Droma, Yun Haixia, Jin Guoen, and Keishi Kubo

Subjects
DNA, Complementary, Molecular Sequence Data, Biology, Molecular cloning, Biochemistry, DNA sequencing, Homology (biology), Hemoglobins, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Gene, Phylogeny, Sheep, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, General Medicine, Ruminants, Sequence Analysis, DNA, biology.organism_classification, Molecular biology, TA cloning, Reverse transcription polymerase chain reaction, Pantholops hodgsonii, Cattle, Hemoglobin, Sequence Alignment
Abstract

To investigate the possible mechanisms of high-altitude native animals in adapting to high altitude, we cloned hemoglobin alpha-chain (alpha-chain Hb) gene from Pantholops hodgsonii, an animal species that indigenously lives at elevations of 3700-5500 m on the Qinghai-Tibetan plateau. Using reverse transcription polymerase chain reaction (RT-PCR) technique, the alpha-chain Hb gene was amplified from total RNA in the liver of the Pantholops hodgsonii. TA cloning technique was used and the PCR product was cloned into pGEM-T vector. The DNA sequence of the gene was highly homologous with sheep (99.1%), goat (98.6%), cattle (95.6%) and human (86.5%). The alpha-chain Hb gene encoded a 142-amino acid protein that could be identified with the homology of alpha-chain Hb protein in sheep (98%), goat (96%), cattle (91%) and human (87%). However, 18 alternations were detected when compared with the alpha-chain Hb gene in human, and 2 in sheep. Moreover, the alterations of a117 GluAsp and alpha 132 AsnSer in important regions were noted in human and sheep, respectively. Phylogenetic analysis suggested that the structure of alpha-chain Hb was highly similar to that in sheep. This study provided essential information for elucidating the possible roles of hemoglobin in adapting to extremely high altitude in Pantholops hodgsonii.

Published
2007

13. Establishment of an Improved Bundle Therapy Procedure for Acute High-Altitude Disease.

Author

Zhang Xuefeng, Ma Si-Qing, Jin Guoen, Pei Zhiwei, and Guo Zhijian

Subjects
*HIGH altitude pulmonary edema, *THERAPEUTICS research, *CEREBRAL edema, *TREATMENT of mountain sickness, *INFLUENCE of altitude, *RESPIRATORY distress syndrome, *X-rays, *PHYSIOLOGY, *PATIENTS, *THERAPEUTICS, *DISEASE risk factors
Abstract

The article discusses hypoxic pathophysiology and high-altitude medicine in China as of December 2012, with a focus on bundle therapy procedures for treating acute high-altitude diseases such as cerebral edema (HACE) and pulmonary edema (HAPE). Topics include patients from the Tibetan Plateau, diagnostic procedures including use of X-rays to determine interstitial lung edema, and the presence of acute respiratory distress syndrome (ARDS). Additional information is presented in tables depicting results of several bundle therapy strategies.

Published
2012

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