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Cu+‐containing materials have great potentials in various applications like adsorptive desulfurization. Nevertheless, their applications are severely obstructed by poor stability of Cu+ in air. Here, we first clarify the mechanism of Cu+ oxidation by first‐principle calculations and demonstrate that moisture accelerates Cu+ oxidation dramatically. Then, the microenvironment of Cu2O‐modified HKUST‐1, a typical metal‐organic framework, is adjusted from hydrophilic to hydrophobic with polydimethylsiloxane coating (producing Cu2O@HK@P). This isolates moisture from pores and enhances the stability of Cu+ significantly even under oxygen atmosphere. Cu+ in Cu2O@HK@P preserves well after exposed to air for 6 months, while Cu2O@HK lose almost all Cu+ for 2 weeks. The optimal Cu2O@HK@P can remove 540 μmol g−1 of thiophene from hydrous fuel, which is much superior to Cu2O@HK (227 μmol g−1) and most reported adsorbents. Our strategy can also be applied to stabilize Cu+ in various materials including zeolite, mesoporous silica, and activated carbon. [ABSTRACT FROM AUTHOR]

Due to the excellent adsorption capacity on CO2, Mg‐MOF‐74 is highly potential for carbon capture. However, the practical application is seriously hindered by the poor hydrolytic stability of Mg‐MOF‐74 even towards trace of moisture. Here we report a strategy by adjusting the microenvironment of Mg‐MOF‐74 from hydrophilic to superhydrophobic by coating polydimethylsiloxane (PDS, yielding M74@P), which markedly enhances the hydrolytic stability. Mg‐MOF‐74 is degraded obviously upon exposure to humid atmosphere (75% relative humidity) for 7 days, while M74@P retains its structure well even after 1 year. The dynamic breakthrough results show that the optimized M74@P sample can capture 6.65 mmol g−1 CO2 from wet CO2/N2 mixtures after 100 cycles, which is superior to Mg‐MOF‐74 (1.17 mmol g−1) and most reported adsorbents under comparable conditions (continuous flow adsorption from wet gases). The excellent adsorption capacity and hydrolytic stability make the present adsorbents highly promising for practical applications in carbon capture. [ABSTRACT FROM AUTHOR]

The article offers information on the establishment and application of nested polymerase chain reaction (PCR) assay for detection of Mycoplamsa hyorhinis and template of DNA that was extracted by phenol-chloroform extraction methods.

Background: Previous studies have proposed that the transcriptional regulatory factor tripartite motif containing 29 (TRIM29) is involved in carcinogenesis via binding with nucleic acid. TRIM29 is confirmed to be highly expressed when the cancer cells acquire therapy-resistant properties. We noticed that TRIM29 levels were significantly increased in anlotinib-resistant NCIH1975 (NCI-H1975/AR) cells via mining data information from gene expression omnibus (GEO) gene microarray (GSE142031; log2 fold change > 1, p < 0.05)., Objective: Our study aimed to investigate the function of TRIM29 on the resistance to anlotinib in non-small cell lung cancer (NSCLC) cells, including NCI-H1975 and A549 cells., Methods: Real-time RT-PCR and western blot were used to detect TRIM29 expression in anlotinib- resistant NSCLC (NSCLC/AR) cells. Apoptosis were determined through flow cytometry, acridine orange/ethidium bromide staining as well as western blot. ELISA was used to measure the content of C-X3-C motif chemokine ligand 1. Co-Immunoprecipitation assay was performed to verify the interaction between TRIM29 and RAD50 double-strand break repair protein (RAD50)., Results: TRIM29 expression was shown to be elevated in the cytoplasm and nucleus of NSCLC/ AR cells compared to normal NSCLC cells. Next, we demonstrated that TRIM29 knockdown facilitated apoptosis and enhanced the sensitivity to anlotinib in NSCLC/AR cells. Based on the refined results citing from the database BioGRID, it was proved that TRIM29 interacted with RAD50. Herein, RAD50 overexpression diminished the pro-apoptotic effect induced by silencing TRIM29 in anlotinib-resistant A549 (A549/AR) cells., Conclusion: Finally, we concluded that the increased sensitivity to anlotinib in NSCLC/AR cells was achieved by knocking down TRIM29, besides, the positive effects of TRIM29 knockdown were attributed to the promotion of apoptosis via binding to RAD50 in NSCLC/AR cell nucleus. Therefore, TRIM29 might become a potential target for overcoming anlotinib resistance in NSCLC treatment., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Aim: To investigate the relationship between p57 and cholesterol in cellular membrane under physiological conditions., Methods: Different concentrations of mbetaCD were used to extract cholesterol from cellular membrane of U937 cells. The cellular membrane was prepared by ultracentrifugation and the content change of p57 in cellular membrane component was measured by Western blot analysis. Discontinuous sucrose density gradient centrifugation was used to prepare lipid rafts and the content of p57 in them was measured by Western blot analysis., Results: The degradation of cholesterol level in cellular membrane didn't lead to obvious content change of p57 in cellular membrane component. Furthermore, no p57 protein was found in lipid rafts., Conclusion: No relationship between p57 and cholesterol in cellular membrane was found in the physiological condition, which is obviously different from that in pathological condition caused by the invasion of pathogenic mycobacteria.

Objective: To observe the association between fasting plasma glucose (FPG) and 5 years outcome post PCI in aged patients with coronary artery disease (CAD)., Methods: A total of 269 patients (mean age 63.8 +/- 9.4 years, 236 males) with CAD underwent PCI between January 2000 and December 2001 were followed up and data on angiographic restenosis, the major adverse cardiac events, the cumulative survival rates and the correlated risk factors were collected and analyzed. Patients were divided into 4 groups according to the levels of their FPG at baseline (group 1: FPG < 5.6 mmol/L; group 2: 5.6 mmol/L < or = FPG < 6.1 mmol/L; group 3: 6.1 mmol/L < or = FPG < 7.0 mmol/L; group 4: FPG > or = 7.0 mmol/L)., Results: At the end of the 5 years follow-up, the incidences of major adverse cardiac events, target lesion revascularization, recurring angina pectoris and angiographic restenosis of group 2 were significantly higher than those of group 1 (P < 0.05) and similar as those in group 3 (P > 0.05). The cumulative survival rates of cardiovascular events of group 2, group 3 and group 4 were all significantly decreased compared with group 1 (P < 0.05). The logistic regression model analysis showed that FPG was an independent risk factor for angiographic restenosis, incidence of major adverse cardiac events, all-cause mortality and recurring angina pectoris (P < 0.05)., Conclusion: FPG > or = 5.6 mmol/L and over is associated with increased incidences of major adverse cardiac events in aged patients with CAD who underwent PCI.

Objective: To assess the prevalence of metabolic syndrome (MS) and its association with mortality of cerebro-cardiovascular diseases in senile population., Methods: Data were collected from 1926 people aged 60 and over, who took part in routine health examination in our hospital from 1996 to 1997. All subjects were followed up for 10 years. MS was diagnosed by using the definition recommended by Chinese Diabetic Society in 2004. Cox-proportional hazards models were used in survival analyses and to calculate the relative risk (RR) of cerebro-cardiovascular diseases mortality., Results: The prevalence of MS was 25.03% (n = 482, Group 2) in this population. The 10 year mortality of cerebro-cardiovascular diseases was significantly higher (6.82/1000-person year vs. 2.55/1000-person year, P < 0.05) and the cumulative survival rate was significantly lower (92.46%vs. 97.14%, P < 0.05) in group 2 compared that in group 1 (non-MS, n = 1444). Compared with group 1, RR of cerebro-cardiovascular diseases mortality was 2.52 (95% CI 1.367 - 4.661, P < 0.05) in group 2., Conclusion: There was a high prevalence of MS in the senile population and MS was associated with higher 10 years mortality of cerebro-cardiovascular diseases.

Objectives: To examine the reproducibility of oral glucose tolerance test (OGTT) in screening abnormal glucose metabolism in Chinese population., Methods: Two times of OGTT were performed in 259 Chinese subjects without a history of diabetes (male 152, female 107) over a period of 2-3 weeks., Results: The overall reproducibility was 56.4%. Subjects with impaired glucose regulation (IGR) at the first test showed lower reproducibility (37.5%), as compared with subjects with normal glucose metabolism (91.2%) and newly detected diabetes (63.8%). The reproducibility of OGTT in group with two components of metabolic syndrome is the lowest (P < 0.05). According to the results of the two tests, subjects with one abnormal OGTT had older, higher systolic blood pressure, heart rate and waist-to-hip ratio (WHR) than those with two normal OGTTs and had lower waist circumference, triglyceride and higher high-density lipoprotein cholesterol than those with two abnormal OGTTs., Conclusions: These findings confirmed the poor reproducibility of OGTT which was lower in subjects with IGR and moderate metabolic abnormality. Furthermore, subjects with one abnormal OGTT, whether reproducible or not, had a higher cardiovascular risk profile, as compared with subjects with normal glucose regulation on two OGTTs. Therefore, OGTT is still essential to the screening of abnormal glucose metabolism.