Search

Your search keyword '"Jin, Yu-Bei"' showing total 10 results
Start Over Author "Jin, Yu-Bei"
10 results on '"Jin, Yu-Bei"'

1. Immune responses of mice inoculated with recombinant Lactobacillus plantarum NC8 expressing the fusion gene HA2 and 3M2e of the influenza virus and protection against different subtypes of influenza virus

Author

Bo, Feng, Yang, Wen-Tao, Shonyela, Seria Masole, Jin, Yu-Bei, Huang, Ke-Yan, Shao, Li-Na, Wang, Cong, Zhou, You, Li, Qiong-Yan, Jiang, Yan-Long, Huang, Hai-Bin, Shi, Chun-Wei, Wang, Jian-Zhong, Wang, Guan, Kang, Yuan-Huan, Yang, Gui-Lian, and Wang, Chun-Feng

Published
2019
Full Text
View/download PDF

9. Expression and purification of swine RAG2 in E. coli for production of porcine RAG2 polyclonal antibodies

Author

Jin, Yu-Bei, primary, Yang, Wen-Tao, additional, Huang, Ke-Yan, additional, Chen, Hong-Liang, additional, Shonyela, Seria-Masole, additional, Liu, Jing, additional, Liu, Qiong, additional, Feng, Bo, additional, Zhou, You, additional, Zhi, Shu-Li, additional, Jiang, Yan-Long, additional, Wang, Jian-Zhong, additional, Huang, Hai-Bin, additional, Shi, Chun-Wei, additional, Yang, Gui-Lian, additional, and Wang, Chun-Feng, additional

Published
2017
Full Text
View/download PDF

10. Expression and purification of swine RAG2 in E. colifor production of porcine RAG2 polyclonal antibodies

Author

Jin, Yu-Bei, Yang, Wen-Tao, Huang, Ke-Yan, Chen, Hong-Liang, Shonyela, Seria-Masole, Liu, Jing, Liu, Qiong, Feng, Bo, Zhou, You, Zhi, Shu-Li, Jiang, Yan-Long, Wang, Jian-Zhong, Huang, Hai-Bin, Shi, Chun-Wei, Yang, Gui-Lian, and Wang, Chun-Feng

Abstract

Recombination activating gene 2 (RAG2) is necessary for immature B cell differentiation. Antibodies to human and rabbit RAG2 are currently commercially available, but antibodies to swine RAG remain unavailable to date. In this study, the swine RAG2 genes sequence was synthesized and then cloned into a pET-28a vector. The recombinant fusion protein was successfully expressed in E. coli,purified through nickel column chromatography, and further digested with Tobacco Etch Virus protease. The cleaved protein was purified by molecular-exclusion chromatography and named pRAG2. We used pRAG2 to immunize rabbits, collected the serum and purified rabbit anti-pRAG2 polyclonal antibodies. The rabbit anti-pRAG2 polyclonal antibodies were tested via immunofluorescence on eukaryotic cells overexpressing pRAG2 and also able to recognize pig natural RAG2 and human RAG2 protein in western blotting. These results indicated that the prepared rabbit anti-pRAG2 polyclonal antibodies may serve as a tool to detect immature B cell differentiation of swine.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results