70 results on '"Jin‑Lan Pan"'
Search Results
2. A Recurrent Cryptic MED14-HOXA9 Rearrangement in an Adult Patient With Mixed-Phenotype Acute Leukemia, T/myeloid, NOS
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Qian Wang, Ling Zhang, Ming-qing Zhu, Zhao Zeng, Bao-zhi Fang, Jun-dan Xie, Jin-lan Pan, Chun-xiao Wu, Ni Wu, Ri Zhang, Su-ning Chen, and Hai-ping Dai
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mixed-phenotype acute leukemia ,MED14-HOXA9 ,fusion gene ,PTPN11 ,NOTCH1 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
To define the fusion genes in T/myeloid mixed-phenotype acute leukemia (T/M MPAL), we performed transcriptome sequencing of diagnostic bone marrow samples from 20 adult patients. Our analysis identified a second instance of a recurrent MED14-HOXA9 chimeric gene resulting from the in-frame fusion of exon 23 of MED14 and exon 1 of HOXA9, the first in an adult patient. The MED14-HOXA9 fusion gene was detected in both the diagnostic and relapsed blasts with reverse transcription-polymerase chain reaction and Sanger sequencing. The patient received combined conventional chemotherapy but suffered relapse at 11 months and died of disease progression one year after the initial diagnosis. Our data suggest that MED14-HOXA9 is a cryptic recurrent aberration in T/M MPAL, which might indicate an aggressive clinical course and inferior outcome after conventional chemotherapy. Further studies will be carried out to reveal the effects of the MED14-HOXA9 fusion on the differentiation and proliferation of leukemia stem cells, as well as suitable treatment strategies for this emerging entity.
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- 2021
- Full Text
- View/download PDF
3. Identification of a Novel MAPK1::BCR Fusion Gene/t(9;22) (q34;q11) in a Case of Acute Promyelocytic Leukemia.
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Qian Wang, Ling-Ji Zeng, Man Wang, Jian-Yu Weng, and Jin-Lan Pan
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FLOW cytometry ,ACUTE promyelocytic leukemia ,PATHOLOGIC complete response ,RARE diseases ,CHROMOSOME abnormalities ,REVERSE transcriptase polymerase chain reaction ,KARYOTYPES ,RNA ,TRETINOIN ,SEQUENCE analysis ,ECCHYMOSIS ,HEMORRHAGE - Published
- 2024
- Full Text
- View/download PDF
4. A new breakpoint fusion gene involving <scp>KMT2A</scp> :: <scp>EDC4</scp> rearrangement in de novo acute myeloid leukemia
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De‐Yuan Hu, Man Wang, Kai Shen, Jin‐Lan Pan, Yu‐Sha Guo, Zhi‐Bo Zhang, Feng‐Hong Zhang, Jia Yin, and Su‐Ning Chen
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Biochemistry (medical) ,Clinical Biochemistry ,Hematology ,General Medicine - Published
- 2023
5. Integrative genomic and transcriptomic profiling reveals distinct molecular subsets in adult mixed phenotype acute leukemia
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Qian Wang, Wen‐zhi Cai, Qin‐rong Wang, Ming‐qing Zhu, Ling‐zhi Yan, Yan Yu, Xie‐bing Bao, Hong‐jie Shen, Hong Yao, Jun‐dan Xie, Tong‐tong Zhang, Ling Zhang, Xiao‐yu Xu, Zhe Shan, Hong Liu, Jian‐nong Cen, Dan‐dan Liu, Jin‐lan Pan, Da‐ru Lu, Jia Chen, Yang Xu, Ri Zhang, Ying Wang, Sheng‐li Xue, Miao Miao, Yue Han, Xiao‐wen Tang, Hui‐ying Qiu, Ai‐ning Sun, Jin‐yan Huang, Hai‐ping Dai, De‐pei Wu, and Su‐ning Chen
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Hematology - Abstract
Mixed phenotype acute leukemia (MPAL) is a subtype of leukemia in which lymphoid and myeloid markers are co-expressed. Knowledge regarding the genetic features of MPAL is lacking due to its rarity and heterogeneity. Here, we applied an integrated genomic and transcriptomic approach to explore the molecular characteristics of 176 adult patients with MPAL, including 86 patients with T-lymphoid/myeloid MPAL (T/My MPAL-NOS), 42 with Ph+ MPAL, 36 with B-lymphoid/myeloid MPAL (B/My MPAL-NOS), 4 with t(v;11q23), and 8 with MPAL, NOS, rare types. Genetically, T/My MPAL-NOS was similar to B/T MPAL-NOS but differed from Ph+ MPAL and B/My MPAL-NOS. T/My MPAL-NOS exhibited higher CEBPA, DNMT3A, and NOTCH1 mutations. Ph+ MPAL demonstrated higher RUNX1 mutations. B/T MPAL-NOS showed higher NOTCH1 mutations. By integrating next-generation sequencing and RNA sequencing data of 89 MPAL patients, we defined eight molecular subgroups (G1-G8) with distinct mutational and gene expression characteristics. G1 was associated with CEBPA mutations, G2 and G3 with NOTCH1 mutations, G4 with BCL11B rearrangement and FLT3 mutations, G5 and G8 with BCR::ABL1 fusion, G6 with KMT2A rearrangement/KMT2A rearrangement-like features, and G7 with ZNF384 rearrangement/ZNF384 rearrangement-like characteristics. Subsequently, we analyzed single-cell RNA sequencing data from five patients. Groups G1, G2, G3, and G4 exhibited overexpression of hematopoietic stem cell disease-like and common myeloid progenitor disease-like signatures, G5 and G6 had high expression of granulocyte-monocyte progenitor disease-like and monocyte disease-like signatures, and G7 and G8 had common lymphoid progenitor disease-like signatures. Collectively, our findings indicate that integrative genomic and transcriptomic profiling may facilitate more precise diagnosis and develop better treatment options for MPAL.
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- 2022
6. [Clinical Features and Prognostic Factors of Patients with Multiple Myeloma]
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Ying-Ying, Gong, Xiao-Shuang, Yan, Ye-Min, Wang, Jin-Lan, Pan, Ying-Ying, Zhai, Su-Ning, Chen, and Dan-Dan, Liu
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Treatment Outcome ,Hematopoietic Stem Cell Transplantation ,Humans ,Middle Aged ,Multiple Myeloma ,Prognosis ,Transplantation, Autologous ,Disease-Free Survival ,Retrospective Studies - Abstract
To summarize the clinical and Laboratory characteristics of patients with multiple myeloma (MM) and analyze the prognostic factors.Two hundred MM patients were retrospectively analyzed for the following parameters, including peripheral blood, bone marrow morphology, cytogenetics, clinical staging, and response to the chemotherapy in order to summarize related factors affecting overall survival (OS). The prognostic factors were also analyzed.200 patients with MM were divided into 3 groups according to bone marrow plasma cell percentage (BMPC%) in bone marrow smears: <10% group (74 cases, 37.0%), 10%-50% group (75 cases, 37.5%), >50% group (51 cases, 25.5%). Compared with the other two groups, patients in BMPC%10% group were characterized by lower clinical staging levels, lower rates of 13q14 deletion and t(11;14) positive, better response to chemotherapy and favorable three-year OS rate. The univariate analysis showed that prognostic factors indicating favorable outcome as evaluated by OS included age≤55 years old, BMPC%<10%, WBC<7.5×10The clinical characteristics are different among MM patients with different BMPC% in bone marrow smears at initial diagnosis, and prognostic analysis shows that the BMPC% in bone marrow smears has an effect on OS rate. BMPC% in bone marrow smears at initial diagnosis, age, WBC, Hb, response to the fourth chemotherapy are also the main factors impacting the prognosis of patients.多发性骨髓瘤患者的临床特征及预后因素分析.总结多发性骨髓瘤(MM)患者的临床及实验室特征资料并分析影响预后的因素.回顾性分析苏州大学附属第一医院收治的200例初诊MM患者外周血、骨髓象、细胞遗传学、临床分期、疗效等方面的特点,总结影响患者总体生存(OS)的相关因素.200例MM患者按照初诊时骨髓涂片获取的骨髓浆细胞百分比(BMPC%)分为3组,<10%组占37.0%(74例),10%-50%组占37.5%(75例),>50%组占25.5%(51例);相较于其他两组,<10%组的患者各项临床分期级别偏低,13q14缺失、t(11;14)阳性率低,对化疗更敏感,3年OS率更高。单因素分析结果显示,初诊时年龄≤55岁、BMPC%<10%、白细胞计数<7.5×10初诊时骨髓涂片BMPC%不同的MM患者临床及实验室特点有所不同;初诊时骨髓涂片BMPC%≤50%、年龄小、无重度贫血、白细胞计数不高、行第四疗程治疗后疗效好为影响患者预后的主要因素;预后分析提示骨髓涂片BMPC%对患者OS率有影响.
- Published
- 2021
7. A Recurrent Cryptic
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Qian, Wang, Ling, Zhang, Ming-Qing, Zhu, Zhao, Zeng, Bao-Zhi, Fang, Jun-Dan, Xie, Jin-Lan, Pan, Chun-Xiao, Wu, Ni, Wu, Ri, Zhang, Su-Ning, Chen, and Hai-Ping, Dai
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Oncology ,fusion gene ,NOTCH1 ,Brief Research Report ,PTPN11 ,mixed-phenotype acute leukemia ,MED14-HOXA9 - Abstract
To define the fusion genes in T/myeloid mixed-phenotype acute leukemia (T/M MPAL), we performed transcriptome sequencing of diagnostic bone marrow samples from 20 adult patients. Our analysis identified a second instance of a recurrent MED14-HOXA9 chimeric gene resulting from the in-frame fusion of exon 23 of MED14 and exon 1 of HOXA9, the first in an adult patient. The MED14-HOXA9 fusion gene was detected in both the diagnostic and relapsed blasts with reverse transcription-polymerase chain reaction and Sanger sequencing. The patient received combined conventional chemotherapy but suffered relapse at 11 months and died of disease progression one year after the initial diagnosis. Our data suggest that MED14-HOXA9 is a cryptic recurrent aberration in T/M MPAL, which might indicate an aggressive clinical course and inferior outcome after conventional chemotherapy. Further studies will be carried out to reveal the effects of the MED14-HOXA9 fusion on the differentiation and proliferation of leukemia stem cells, as well as suitable treatment strategies for this emerging entity.
- Published
- 2021
8. [Clinical Significance of Common Gene Mutations in 53 Patients with Acute Myeloid Leukemia Harboring 11q23/MLL Rearrangements]
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Shu-Xiao, Bai, Yan-Lei, Gong, Jing-Ren, Zhang, Chun-Xiao, Wu, Jun, Zhang, Hui-Ying, Qiu, Hong-Jie, Shen, Jian-Nong, Cen, Su-Ning, Chen, and Jin-Lan, Pan
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Leukemia, Myeloid, Acute ,fms-Like Tyrosine Kinase 3 ,Chromosomes, Human, Pair 11 ,Mutation ,Hematopoietic Stem Cell Transplantation ,Humans ,Prognosis ,Nucleophosmin ,In Situ Hybridization, Fluorescence - Abstract
To investigate the clinical significance of AML patients with 11q23/MLL rearrangement, and to evaluate the effect of those mutations on the AML patients.53 cases involving translocations of chromosome 11q23 were identified by chromosome banding analysis. MLL rearrangements were detected by fluorescence in situ hybridization and/or multiplex nested PCR. The samples were screened for mutations in the candidate genes FLT3-ITD, FLT3-TKD, TET2, N-RAS, ASXLI, EZH2, DNMT3, C-Kit, NPM1, WT1, CEBPA by using genomic DNA-PCR and deep-sequencing.21/53 MLL-rearranged AML cases showed at least one additional chromosomal aberrations. The most common additional aberration was +8. Gene mutations were observed in 23 cases (43.4%) and most cases showed singal mutation. N-RAS mutation was more frequent (8 cases, 15.1%), followed by WT1 mutation in 4 cases (7.5%), FLT3-ITD mutation in 3 cases, ASXL1 mutation in 2 cases, DNMT3A mutation in 2 cases, EZH2 mutation in 1 case, c-Kit17 mutation in 1 case, FLT3-TKD mutation in 1 case, and FLT3-ITD and TKD mutation coexistent in 1 case. No mutation was detected in CEBPA, NPM1, C-KIT8, TET2. Median OS for gene mutated patients was 8.5 months and 13 months for no mutated patients. Median OS for patients who received hematopoietic stem cell transplantation (HSCT) was 22.5 months and 7.5 months for patients who olny received chemotherapy.A relatively high mutation frequency is observed in AML patients with 11q23/MLL rearrangements and most cases shows single mutation. The RAS signaling pathway alterations are most common. Gene mutation does not affect the OS of these patients, who show poor prognosis. A significantly higher Hb at initial diagnosis in FLT3 mutated patients is significantly higher than that in FLT3 wild-type cases. Patients who underwent HSCT show a better prognosis than those only received chemotherapy.53例伴有11q23/MLL重排的急性髓系白血病的常见基因突变.了解AML中常见基因突变在11q23/MLL重排的急性髓系白血病中的发生率,探讨伴有基因突变的11q23/MLL重排AML患者的临床特点,评估这些基因突变对该类AML患者的预后影响.53例AML患者的核型均涉及11q23易位,采用FISH和/或多重PCR进行MLL重排检测;采用基因组DNA-PCR技术对该组标本进行AML中11种常见基因突变的检测,这些基因包括:FLT3-ITD、FLT3-TKD、TET2、N-RAS、ASXL1、EZH2、DNMT3、C-Kit、NPM1、WT1、CEBPA等.53例患者均为MLL重排,其中21例(39.6%)同时合并有其它染色体异常,最常见的为+8;23例(43.4%)为突变阳性,均为单一突变。其中N-RAS突变发生率最高,为8例(15.1%),其次为WT1为4例(7.5%)、FLT3-ITD突变3例、ASXL1突变2例,DNMT3A突变2例,EZH2突变1例,c-Kit17突变1例,FLT3-TKD突变1例,FLT3-ITD和TKD双突变1例,而CEBPA、NPM1、C-KIT8、TET2 等基因在本组病例中未发现突变。基因突变患者的中位总生存期为8.5个月,未突变患者的中位总生存期为13个月。18例患者行造血干细胞移植,其中位总生存期为22.5个月,仅行化疗患者的OS时间为7.5个月.11q23/MLL重排AML基因突变发生率较高,但多为单一突变,其中RAS通路基因突变最常见。该类患者预后不良,各种基因突变并不影响该类患者的总生存期,FLT3突变患者初诊时血红蛋白高于FLT3野生型患者,行造血干细胞移植可使该类患者获得较好预后.
- Published
- 2020
9. [Clinical Features and Prognosis of 188 Patients with Acute Myeloid Leukemia-M
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Jing-Jing, Wang, Chao, Wang, Xiao-Shuang, Yan, Jin-Lan, Pan, Ming-Qing, Zhu, Jian-Nong, Cen, Su-Ning, Chen, and Dan-Dan, Liu
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Leukemia, Myeloid, Acute ,Mutation ,Humans ,HLA-DR Antigens ,Middle Aged ,Prognosis ,Immunophenotyping ,Retrospective Studies - Abstract
To summarize the clinical characteristics of patients with acute myeloid leukemia-type MOne hundred eighty-eight AML-MAmong 188 patients with AML-MThe clinical characteristics are different between M188例急性髓系白血病M总结急性髓系白血病 M通过回顾性分析本血液病研究中心收治的 188例急性髓系白血病 M在188例M伴有不同核型异常的M
- Published
- 2019
10. [Effect of Additional Chromosomal Abnormalities on the Outcome of CML-CP Patients Receiving TKI Therapy]
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Yan-Hua, Yue, Xue-Feng, He, Jin-Lan, Pan, Jun, Zhang, Chao, Xu, Li, Yao, Yan, Chen, Su-Ning, Chen, and Jian-Nong, Cen
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Chromosome Aberrations ,Treatment Outcome ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Humans ,Antineoplastic Agents ,Follow-Up Studies - Abstract
To explore the effect of additional chromosomal abnormalities on the prognosis and outcome of CML-CP patients receiving imatinib therapy.The clinical and genetic data of 589 CML-CP patients receiving imatinib treatment between May 2009 and October 2014 in the 1st Affiliated Hospital of Soochow University were analyzed, the 589 patients were divided into 5 groups according to the karyotypes at the initial diagnosis. The OS(overall survival), PFS (progression-free survival), EFS (event-free survival), Cumulative MMR (major molecular remission) and Cumulative CCyR (complete cytogenetic remission) were calculated by using the Kaplan-Meier method and compared by using the log-rank text by Graphpad 6.0. The χThere was significant difference about the frequency of optimal molecular response at 3 and 6 months between CML-CP patients with additional chromosomal abnormalities and those with classic t(9;22) [50%(12/24) vs. 73.94%(261 /353), P0.05; 50%(10 /20) vs. 72.05%(232 /322) (P0.05)], and the same significant difference was found at 6 months between the group with variant translocations and that with classic t(9;22) [53.3% (16 /30) vs. 72.05%(232 /322) (P0.05)]. The P values of cumulative CCyR (P0.05) and EFS (P0.01) for 4 years were statistically significant between CML-CP patients with additional chromosomal abnormalities and the other 4 groups. Compared one to another, there was the significant difference in cumulative CCyR and EFS for 4 years between CML-CP patients with additional chromosomal.abnormalities and those with classic t(9;22) (47.25% vs. 84.01%)(P0.05); (75.03% vs. 90.01%)(P0.01).The additional chromosomal abnormalities influence the outcome of CML-CP patients receiving imatinib treatment, which make poor prognosis.
- Published
- 2018
11. Coexistence of p210BCR‑ABL and CBFβ‑MYH11 fusion genes in myeloid leukemia: A report of 4 cases
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Feng Jiang, Zi‑Xing Chen, Dan‑Dan Liu, Jian‑Ying Liang, Jian‑Nong Cen, Xiao‑Fei Qi, Su‑Ning Chen, Yuanyuan Wang, Wen‑Jing Ding, and Jin‑Lan Pan
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Cancer Research ,ABL ,Myeloid leukemia ,Articles ,Biology ,medicine.disease ,Fusion protein ,myeloid leukemia ,Fusion gene ,Transplantation ,03 medical and health sciences ,Leukemia ,p210BCR-ABL ,0302 clinical medicine ,Oncology ,hemic and lymphatic diseases ,030220 oncology & carcinogenesis ,Acute lymphocytic leukemia ,Immunology ,corebinding factor β-myosin heavy chain 11 fusion genes ,medicine ,MYH11 ,Cancer research ,neoplasms ,030215 immunology - Abstract
Numerous acquired molecular and cytogenetic abnormalities are strongly associated with hematological malignancies. The breakpoint cluster region-ABL proto-oncogene 1 (BCR-ABL) rearrangement leads to a p210 chimeric protein in typical chronic myeloid leukemia (CML), whereas 17–25% of patients with acute lymphocytic leukemia and 0.9–3% patients with de novo acute myeloid leukemia (AML) carry a p190BCR-ABL fusion protein. Cases of patients with AML/CML carrying two specific primary molecular changes, BCR-ABL and core binding factor-β-myosin heavy chain 11 (CBFβ-MYH11) fusion genes have been rarely reported. The present study aimed to understand the nature and mechanism of this particular type of leukemia through case reports and literature review. A total of four patients who were diagnosed as AML/CML with BCR-ABL and CBFβ-MYH11 fusion genes in the First Affiliated Hospital of Soochow University (Suzhou, China) between January 2004 and December 2012 were examined. Morphological analysis of bone marrow cells, flow cytometry, quantitative polymerase chain reaction of p210BCR-ABL and CBFβ-MYH11 transcripts as well as cytogenetic and fluorescence in situ hybridization analyses were performed. A total of 4 patients who exhibited fusion of p210BCR-ABL and CBFβ-MYH11 were identified. A single patient (case 1) was first diagnosed CML-acute phase (AP), which progressed rapidly to CML-blast crisis (BC), and three patients (cases 2, 3 and 4) were diagnosed with AML with bone marrow eosinophilia at first presentation with no evidence of previous onset of CML. All cases achieved remission following conventional chemotherapy/hematological stem cell transplantation combined with the inhibitor of tyrosine kinase (TKI) maintenance therapy. The patients with CML carrying and expressing BCR-ABL and CBFβ-MYH11 fusion genes appeared more likely to rapidly progress to AP or BC. Therefore, the product of the CBFβ-MYH11 fusion gene may serve an important role in the transformation of CML. The co-expression of p210BCR-ABL and CBFβ-MYH11 fusion genes in myeloid leukemia may be a molecular event occurring not only during the development of CML, but also in AML.
- Published
- 2017
12. [Autophagy Activity of CD34+ Cells in MDS Patients and Its Clinical Significance]
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Feng, Jiang, Yuan-Yuan, Wang, Jian-Nong, Cen, Zi-Xing, Chen, Jian-Ying, Liang, Dan-Dan, Liu, Jin-Lan, Pan, Ming-Qing, Zhu, and Su-Ning, Chen
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Leukemia, Myeloid, Acute ,Myelodysplastic Syndromes ,Autophagy ,Humans ,Antigens, CD34 ,Bone Marrow Cells ,Flow Cytometry ,Prognosis - Abstract
To explore the autophagy activity of CD34+ cells in bone marrow of MDS patients and its clinical significance.The activity of autophagy in bone marrow CD34+ cells from 20 MDS patients, 20 non-malignant anemia patients and 5 AML patients admitted in our hospital from October 2012 to March 2014 was detected by flow cytometry (FCM).The autophagy activity in low risk MDS patients and non-malignant anemia patients were both significantly higher than that in both high risk MDS and AML patients (P0.05), and more interestingly, the autophagy activity in MDS negatively correlated with World Health Organization classification-based prognostic system (WPSS) score (r=-0.877) .The autophagy activity CD34+ cells in the patients with MDS is higher than that in AML patients, and negatively correlated with WPSS scores, indicating that the decrease of autophagy activity maybe accelerate the genesis and development of MDS and relate with the prognosis of MDS patients.
- Published
- 2016
13. Contents Vol. 120, 2008
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G.R. Degasperi, Woosung Yu, S.T.O. Saad, In Keun Choi, A. Christoforidou, Seungjin Lee, In Sun Kim, A. Anastasiadis, G. Bourikas, Sanghui Park, W.P. Pfeifer, Jin-Lan Pan, Byung Soo Kim, Kyoung-Mee Kim, Seok Kim, Ya-Fang Wu, Ashis Mukhopadhyay, Ofer Shpilberg, M.T. Almeida, Uma B. Dasgupta, Mical Paul, Bama Charan Mondal, Anat Gafter-Gvili, Jun Haeng Lee, A. Goutzouvelidis, Yong-Quan Xue, Bhaswati Ganguli, Ronit Gurion, Chul Won Choi, Jae J. Kim, Jun Suk Kim, V. Kaloutsi, Young-Hyeh Ko, I. Kotsianidis, Hai-Ping Dai, Jong-Chul Rhee, C. Tsatalas, Moshe Yeshurun, Ron Ram, D. Margaritis, Ki-Hyun Kim, Hee Sang Hwang, Juan Shen, A.E. Vercesi, Jun Zhang, Young Hyeh Ko, Yong Wang, F.F. Costa, Won Kim, Hwa Jung Sung, Pia Raanani, D. Pantelidou, E. Spanoudakis, and Utpal Chaudhuri
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Hematology ,General Medicine - Published
- 2008
14. [Clinical and Laboratorial Characteristics of Primary Acute Myeloid leukemia with Philadelphia Chromosome and Inversion 16]
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Feng, Jiang, Yuan-Yuan, Wang, Zi-Xing, Cheng, Su-Ning, Chen, Dan-Dan, Liu, Jian-Ying, Liang, Jin-Lan, Pan, Ming-Qing, Zhu, Wen-Jing, Ding, and Jian-Nong, Cen
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Chromosome Aberrations ,Leukemia, Myeloid, Acute ,Chromosome Inversion ,Fusion Proteins, bcr-abl ,Humans ,Chromosome Disorders ,Philadelphia Chromosome ,HLA-DR Antigens - Abstract
To summarize the clinical characteristics as well as diagnosis and treatment in 1 case of acute myeloid leukemia(AML) with coexpression of Ph and inv(16).A series of clinical tests, the cellular morphological, immunological, cytogenetic and molecular biological examinations of leukemia cells were performed.The clinical characteristics of this patient were very common. The cellular morphology is similar to the AML with inv(16). The leukemia cells were stained positively for CD13, CD33, CD34, CD117 and HLA-DR. Karyotypic analysis showed a complex chromosome abnormality including inv(16) and Ph, and the FISH analysis showed that the percentage of rearrangement of CBFβ allele was over that of the BCR-ABL fusion signals. The obvious adverse events did not occur in this patient within 3 years.Ph as secondary aberration of inv(16) rarely occures in primary AML cases, and so far there have not been the clear criteria of diagnosis and treatment. The cytogenetic and molecular biology could provide the basis for diagnosis. Moreover, autologous hematopoietic stem cell transplantation combined with imatinib probably is one of the effective treatment methods.
- Published
- 2015
15. [Correlation between expression of SIL-TAL1 fusion gene and deletion of 6q in T-cell acute lymphoblastic leukemia]
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Qian, Wang, Li-Li, Wu, Hai-Ping, Dai, Na-Na, Ping, Chun-Xiao, Wu, Jin-Lan, Pan, Jian-Nong, Cen, Hui-Ying, Qiu, and Su-Ning, Chen
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Comparative Genomic Hybridization ,Oncogene Proteins, Fusion ,Reverse Transcriptase Polymerase Chain Reaction ,Humans ,Leukemia-Lymphoma, Adult T-Cell ,Chromosomes, Human, Pair 6 ,Chromosome Deletion ,Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - Abstract
The present study was designed to investigate the prevalence and clinical significance of SIL-TAL1 rearrangements in T-cell acute lymphoblastic leukemia (T-ALL). The incidence of SIL-TAL1 rearrangements was analyzed by nest real-time quantitative polymerase chain reaction (RT-PCR) in 68 patients with T-ALL. Karyotypic analysis was performed by conventional R-banding assay and array-based comparative genomic hybridization (array-CGH). The results showed that SIL-TAL1 rearrangements were identified in 10/26 (38.5%) pediatric and 2/42 (4.8%) adult T-ALL cases, which indicate a pediatric preference for SIL-TAL1 rearrangements in T-ALL. Two different transcripts were detected in 6/12(50%) T-ALL samples. Abnormal karyotypes were detected in 6 out of 11 cases (54.5%) and a deletion of the long arm of chromosome 6 was observed in 4 cases. Array-CGH results of 2 T-ALL cases with SIL-TAL1 rearrangement revealed that this fusion gene was resulted from a cryptic deletion of 1p32, and the overlap region of 6q deletion was 6q14.1-16.3. These cases with SIL-TAL1 fusion had a higher white blood cell (WBC) count and higher serum levels of lactate dehydrogenase (LDH) than cases without SIL-TAL1 fusion. It is concluded that SIL-TAL1 rearrangements are associated with loss of heterozygosity of chromosomal 6q, and SIL-TAL1-positive patients are younger than SIL-TAL1-negative patients. In contrast to the cases without SIL-TAL1 fusion, there are many adverse prognostic factors in the cases with SIL-TAL1 fusion, such as higher WBC count and higher LDH levels.
- Published
- 2014
16. [Clinical and laboratorial analysis for 15 adult cases of mixed phenotypic acute leukemia with Ph chromosome and/or positive BCR-ABL]
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Ling-Zhi, Yan, Su-Ning, Chen, Na-Na, Ping, Qin-Rong, Wang, Hong, Liu, Zi-Xuan, Ding, Ming-Qing, Zhu, Jian-Ying, Liang, Dan-Dan, Liu, Jian-Nong, Cen, Jin-Lan, Pan, Hui-Ying, Qiu, Ai-Ning, Sun, and De-Pei, Wu
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Adult ,Aged, 80 and over ,Male ,Adolescent ,Fusion Proteins, bcr-abl ,Hematopoietic Stem Cell Transplantation ,Antigens, CD34 ,Middle Aged ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Prognosis ,Survival Rate ,Young Adult ,Phenotype ,Karyotyping ,Humans ,Female ,Protein Kinase Inhibitors ,Aged ,Retrospective Studies - Abstract
The purpose of this study was to summary the clinical and laboratorial features in 15 adult cases of mixed phenotypic acute leukemia with Ph chromosome and/or BCR-ABL fusion gene positive (Ph(+)MPAL), 15 adult patients with Ph(+)MPAL were defined by WHO-2008 classification. The clinical characteristics, results of morphology, immunology, cytogenetics and molecular genetic detections and results of follow-up in 15 adult patients with Ph(+)MPAL were analyzed retrospectively. The results showed that 15 patients among 87 cases of MPAL demonstrated Ph(+)MPAL (17.2%; 15/87) (7 males and 8 females), their median age was 51 (range 16-81) year old and median WBC count at diagnosis was 69 (12.7-921)×10(9)/L. Based on FAB criteria, these patients showed different morphologic types, including AML (13.3%; 2/15), ALL (40.0%; 6/15), HAL (46.7%; 7/15). Immunologic analysis indicated that 15 cases of Ph(-)MPAL were all classified as B-lymphoid +myeloid mixed immunophenotype. Except one patient, all expressed CD34 antigen on the surface of leukemia cells with 64.3% strong positive, only Ph (53.3%; 8/15), Ph with additional chromosomal abnormalities (33.3%; 5/15) and normal karyotype (13.3%; 2/15) were cytogenetically identified. BCR-ABL fusion gene transcript positive were detected by multiplex reverse transcription PCR in all cases, with e1a2 subtype (p190) (40.0%; 6/15) and b2a2 or b3a2 (p210) subtype (60.0%; 9/15). Four out of 7 (57.1%) patients were found to have IKZF1 gene deletion, without other common gene mutations. Seven out of 10 cases (70.0%) achieved complete remission (CR) after one cycle of induction chemotherapy. In the induction stage, CR rate seemed higher when tyrosine kinase inhibitors (TKI) were added to chemotherapy (83.3%:50.0%; P = 0.206). Overall survival (OS) in 4 patients received allogeneic hematopoietic stem cell transplantation (allo-HSCT) was longer than that in 4 patients received chemotherapy alone (P = 0.004). It is concluded that Ph(+)MPAL mainly is expressed as B+My phenotype. The majority of patients is older and has CD34 overexpression. In the aspect of molecular genetics, the Ph(+)MPAL is similar to other acute leukemia with Ph chromosome. Ph(+)MPAL is a subtype of acute leukemia with poor prognosis. WBC count at diagnosis is an independent prognostic factor. The combination of TKI and allo-HSCT can improve their long-term survival, which needs to be confirmed through carrying out a prospective and multicenter clinical trial for newly diagnosed Ph(+)MPAL.
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- 2013
17. [Deletions and rearrangements of PAX5 gene in B-lineage acute lymphoblastic leukemia]
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Ji-fu, Zheng, Sha-sha, Dong, Qian, Wang, Jin-lan, Pan, Su-ning, Chen, and Hui-ying, Qiu
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Adult ,Gene Rearrangement ,Male ,Adolescent ,PAX5 Transcription Factor ,Middle Aged ,Young Adult ,Acute Disease ,Leukemia, B-Cell ,Humans ,Female ,Child ,Chromosomes, Human, Pair 9 ,In Situ Hybridization, Fluorescence ,Sequence Deletion - Abstract
To determine the frequency paired-box domain 5 (PAX5) gene alterations in B-lineage acute lymphoblastic leukemia (B-ALL) harboring 9p abnormalities and its implication for clinical prognosis.Bacterial artificial chromosomes RP11-344B23 and RP11-652D9 encompassing the PAX5 gene were selected. DNA was extracted with conventional method and labeled with fluorescein by nicking transition. Fluorescence in situ hybridization (FISH) was used to determine the rearrangement or deletion of the PAX5 gene in B-ALL harboring chromosome 9p abnormalities. Clinical and laboratory features of patients were analyzed.Fifty cases were analyzed with FISH. Complete deletion was observed in 23 patients (46%), partial deletion was observed in 2 patients (4%), and rearrangement was detected only in 1 case. The total frequency of abnormalities was 52% (26/50). No significant difference was found in clinical features of patients with or without PAX5 rearrangement or deletion.The frequency of PAX5 gene alterations in B-ALL harboring 9p abnormalities was 52%. However, no significant difference was found between patients with and without PAX5 alterations.
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- 2013
18. Clinical and experimental characteristics of 20 patients with acute myeloid leukemia with complex variant of t(821)
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Jing, Xia, Su-Ning, Chen, Jin-Lan, Pan, Qin-Rong, Wang, Ya-Fang, Wu, Yong, Wang, Jun, Zhang, Juan, Shen, Yong-Quan, Xue, and Chang-Geng, Ruan
- Subjects
Adult ,Male ,Adolescent ,Chromosomes, Human, Pair 21 ,DNA Mutational Analysis ,Middle Aged ,Protein-Tyrosine Kinases ,Translocation, Genetic ,Leukemia, Myeloid, Acute ,Young Adult ,Humans ,Female ,Aged ,Chromosomes, Human, Pair 8 ,Retrospective Studies - Abstract
This study was aimed to summarize and analyze the morphology, immunophenotype, cytogenetics, molecular biology (MICM), tyrosine kinase (TK) gene mutations and clinical features of acute myeloid leukemia (AML) with complex variant of t(8;21). A retrospective study was performed for 20 AML patients with complex variant of t(8;21) in our hospital from January 1994 to April 2012, including analysis of clinical feature, immunophenotype, chromosome karyotype, treatment regimen, as well as the overall survival (OS) and relapse-free survival (RFS). Mutations of C-KIT, FLT3-ITD, FLT3-TKD and JAK2V617F were detected by genomic DNA PCR and the sequencing was per-formed in 13 AML patients with complex variant of t(8;21). The results showed that (1) the incidence of 20 AML patients with complex variant of t(8; 21) was 2.4% of total t(8; 21) AML patients. In 20 AML patients with complex variant of t(8;21), 1 case was M1, 17 cases were M2, 2 cases were M4; 10 cases were myeloid phenotype and the other 3 were myeloid plus lymphoid phenotype. There were 16 kinds of cytogenetics additional involvement of chromosomal breakpoints: lp22, 1p32, 2q35, 2q14, 3p25, 5q13, 6p22, 7q21, llq11, 1lq13, 12q14, 12q24, 12p12, 14q32, 15p13, 20q12. (2) C-KIT aberrations were detected in 30.8% cases, all mutated in exon 17 (mutkit 17), only 1 case had JAK2V617F mutation. The result of FLT3 mutation screenings in AML patients with complex variant of t(8; 21) was negative. Of 5 patients with gene mutations, 1 patient (20%) achieved complete remission (CR), the median RFS and median OS time were 6.5 months and 8.9 months respectively. Of the 8 patients without gene mutations, 6 patietns (75%) achieved CR; the median RFS and median OS time were 26.6 months and 27.7 months respectively. It is concluded that the AML patients with complex variant of t(8;21) shows typical features of t(8;21) AML, but the existence of the tyrosine kinase-related gene mutation has important implications on remission rate and long-term survival of patients treated by induction chemotherapy.
- Published
- 2013
19. [Multivariate analysis of imatinib resistance-related factors during the treatment of chronic myeloid leukemia]
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Min, Zhou, Hui-ying, Qiu, Guang-sheng, He, Yang, Xu, Jian-nong, Cen, Jin-lan, Pan, Su-ning, Chen, Ai-ning, Sun, Ri, Zhang, and De-pei, Wu
- Subjects
Adult ,Male ,Adolescent ,Middle Aged ,Piperazines ,Young Adult ,Pyrimidines ,Drug Resistance, Neoplasm ,Child, Preschool ,Benzamides ,Leukemia, Myeloid, Chronic-Phase ,Imatinib Mesylate ,Humans ,Female ,Child ,Aged ,Follow-Up Studies ,Retrospective Studies - Abstract
To explore efficacy of imatinib for patients with chronic myeloid leukemia(CML) and its resistance-related factors during the treatment.The clinical data of 214 CML patients received imatinib were analyzed respectively in our hospital from April 2005 to December 2010. The therapy history and efficacy of regular follow-up and factors influencing drug resistance were analyzed. COX regression analysis was used to perform the univariate and multivariate analysis.Until the end of follow up, thirty-one patients (14.5%) occurred drug resistance. One of them was in accelerated phase(AP), and two in blast phase(BP); 69.2% of patients achieved a complete cytogenetic response(CCyR), and 31.3% of patients achieved a major molecular response(MMR). COX analysis was performed in 207 chronic phase(CP) patients. Univariate analysis showed that the course of disease before treatment, the hemoglobin count, the white blood cell count, whether achieved CCyR or not and whether achieved MMR or not were the influencing factors for imatinib resistance. Multivariate analysis showed that whether achieved CCyR or not was the independent factor for drug resistance.Whether achieved CCyR or not is an independent factor and also a protective factor for imatinib resistance in patients with CML.目的 探讨伊马替尼治疗慢性髓性白血病(CML)过程中发生耐药的相关因素。方法 回顾性分析2005年4月至2010年12月接受伊马替尼治疗的214例CML患者临床资料,随访患者治疗情况、疗效,分析影响耐药的各种因素。应用COX比例风险回归模型对影响耐药的各种因素进行单因素和多因素分析。结果 截至随访终点,31例(14.5%)患者出现伊马替尼耐药,其中1例为加速期患者,2例为急变期患者;完全细胞遗传学反应(CCyR)率为69.2%,主要分子学反应(MMR)率为31.3%。以207例慢性期患者进行COX分析,单因素分析显示,治疗前病程、HGB水平、WBC、是否获得CCyR、是否获得MMR为伊马替尼耐药的影响因素。多因素COX回归分析表明,是否获得CCyR为伊马替尼耐药的独立影响因素。结论 是否获得CCyR是伊马替尼治疗CML是否发生耐药的独立影响因素,且为保护因素。
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- 2013
20. [The different characteristics of ABL kinase domain mutation in the Chinese Han nationality imatinib resistant Philadelphia chromosome-positive acute lymphoblastic leukemia and chronic myeloid leukemia]
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Hong-jie, Shen, Jun, He, Qiao-cheng, Qiu, Jian-nong, Cen, Jin-lan, Pan, Li, Yao, Zi-xuan, Ding, Yan, Chen, and Zi-xing, Chen
- Subjects
Adult ,Chromosome Aberrations ,Adolescent ,Middle Aged ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Protein-Tyrosine Kinases ,Piperazines ,Young Adult ,Pyrimidines ,Asian People ,Drug Resistance, Neoplasm ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Benzamides ,Mutation ,Imatinib Mesylate ,Humans ,Female ,Philadelphia Chromosome ,Proto-Oncogene Proteins c-abl ,Aged - Abstract
To identify the distribution and differentiation of ABL kinase domain mutation in the Chinese Han nationality imatinib resistant chronic myeloid leukemia (CML) and Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph(+)ALL).Bone marrow or peripheral blood samples of 112 imatinib resistant CML patients and 21 Ph(+)ALL patients were obtained from the first affiliated hospital of Soochow university according to local law. Total RNA was extracted from the mononuclear cells using a TRIzol reagent. ABL kinase domain (KD) mutation was detected by direct sequencing.Of the 112 imatinib resistant CML patients, 54.46%(61 cases) had ABL KD mutation. Twenty-three mutants were identified in 20 amino acid sites and 23.21% (26 cases) ABL KD mutations were in P-loop region. ABL KD mutations were also detected in 71.43% (15 cases) imatinib resistant Ph(+)ALL patients, with 10 mutations in 8 amino acid sites. The most frequent mutation was T315I (28.57%), followed by E255K/V (19.05%) and Y253F/H (14.29%). The frequency of T315I was much higher in imatinib resistant Ph(+) ALL than that in imatinib resistant CML (P = 0.001). Ph(+)ALL with additional chromosomal aberrations also had a higher rate of ABL KD mutation than that of CML (P = 0.010). Ph(+)ALL gained ABL KD mutation faster than CML (P0.010).Chinese imatinib resistant CML and Ph(+)ALL patients had different characteristics in ABL KD mutation. The rate of ABL KD mutation in Ph(+)ALL with additional chromosomal aberrations was much higher than that of CML with additional chromosomal aberrations.
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- 2013
21. [Immunophenotyping and molecular genetic analysis of diffuse large B-cell lymphoma]
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Yong-sheng, Han, Yong-quan, Xue, Hai-yan, Yang, Jun, Zhang, and Jin-lan, Pan
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Adult ,Aged, 80 and over ,Male ,Adolescent ,Genes, myc ,Middle Aged ,Genes, bcl-2 ,Immunophenotyping ,DNA-Binding Proteins ,Proto-Oncogene Proteins c-bcl-6 ,Humans ,Female ,Lymphoma, Large B-Cell, Diffuse ,In Situ Hybridization, Fluorescence ,Aged - Abstract
To perform immunophenotyping and molecular genetic analysis for diffuse large B-cell lymphoma (DLBCL), and to explore their correlation and implication for prognosis.Immunohistochemical streptavidin peroxidase (SP) method was used to determine the expression of CD10, BCL6 and MUM1 in 59 cases of DLBCL. A Hans algorithm was used to classify DLBCL into germinal center B-cell (GCB) and non-GCB subtypes. Interphase fluorescence in situ hybridization (FISH) assay was performed on paraffin-embedded lymphoma tissue sections to detect translocations and amplifications of BCL6, BCL2 and MYC genes with dual-color break-apart BCL6 probe, dual-color dual-fusion IgH/ BCL2 probe and dual-color break-apart MYC probe, respectively.In the 59 cases of DLBCL, 28.8% (17/59) belonged to GCB subtype, and 71.2% (42/59) belonged to non-GCB subtype. The incidences of BCL6, BCL2 and MYC gene translocations were 24.1% (14/58), 1.7% (1/59) and 5.3% (3/57), respectively. The incidences of BCL6, BCL2 and MYC gene amplifications were 17.2% (10/58), 22.0% (13/59) and 21.1% (12/57), respectively. BCL6 amplification was not correlated with BCL6 translocation (P=0.424), but was correlated with amplifications of BCL2 and MYC (C=0.405 and 0.403, respectively, P0.01). The incidence of BCL6 translocation in GCB type was higher than that in non-GCB type, and amplifications of BCL6, BCL2 or MYC were more frequently encountered in non-GCB type, though no statistical significance was detected (P=0.089 and 0.106, respectively). By univariate analysis, immunophenotyping and international prognostic index (IPI) exerted a significant effect on overall survival (OS) (P=0.047 and 0.001, respectively), but to which BCL6 translocation and amplification of the 3 genes were not related (P=0.150 and 0.444, respectively). By multivariate analysis, IPI score was the only independent prognostic factor for OS (RR =3.843, P=0.017).The GCB subtype of DLBCL is less common in the patient cohort. Common genetic aberrations have included BCL6 translocation and BCL6, BCL2 and MYC amplifications. Amplification of the 3 genes is strongly correlated with each other, and the incidence of BCL2 translocation is low. Immunophenotyping only has minor significance for the prognosis. Genetic aberrations cannot predict the clinical outcome of DLBCL.
- Published
- 2013
22. [Clinical and laboratory investigation of pericentric inv(9)(p22q34) with the der(9)t(9;22)(q34;q11) in Ph-positive leukemia]
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Yisun, Fan, Shuang-shuang, Ding, Jin-lan, Pan, Yong-quan, Xue, and Zhen-hua, Hu
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Adult ,Male ,Leukemia, Myeloid, Acute ,Chromosomes, Human, Pair 22 ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Chromosome Inversion ,Humans ,Female ,Middle Aged ,Chromosomes, Human, Pair 9 ,Translocation, Genetic - Abstract
To investigate clinical and molecule genetics features of four Ph-positive leukemia patients characterized by pericentric inv(9)(p22q34) with the der(9)t(9;22)(q34;q11).Cytogenetic analysis was carried out on bone marrow directly or after short-period culture. R banding was used for karyotype analysis. BCR/ABL fusion gene was detected with interphase fluorescence in situ hybridization (FISH), and chromosome painting was carried out using specific probes. RT-PCR was used to detect BCR/ABL chimeric transcripts.One patient with acute myeloid leukemia (AML) presented three clones, which included one with a normal karyotype, one with t(9;22)(q34;q11), and one with inv(9)(p22q34) involving the der(9)t(9;22) and additional t(8;12)(q12;p11). The inv(9)(p22q34) has always co-occurred with der(9)t(9;22)(q34;q11) accompanied by der(22)t(9;22)(q34;q11) in all metaphases from the three patients with chronic myeloid leukemia (CML). B3a2 transcript was detected in all patients by RT-PCR. Inv(9)(p22q34) was found in both CML and AML, and was associated with poor prognosis.Inv(9)(p22q34) is a novel, rare, but recurrent secondary chromosomal abnormality for Ph-positive leukemia. Leukemia with der(9)t(9;22) and inv(9)(p22q34) has unique clinical and laboratory characteristics.
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- 2013
23. [Clinical significance of common leukemia gene mutations in patients with acute promyelocytic leukemia]
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Jia, Yin, Ai-Ning, Sun, Xiao-Peng, Tian, Hong, Tian, Rong-Xian, Wang, Zhen, Yang, Xiu-Li, Wang, De-Pei, Wu, Hui-Ying, Qiu, Jin-Lan, Pan, Jian-Nong, Cen, Jian-Ying, Liang, and Su-Ning, Chen
- Subjects
Adult ,Male ,Adolescent ,DNA Mutational Analysis ,Polycomb Repressive Complex 2 ,Nuclear Proteins ,Middle Aged ,Prognosis ,Dioxygenases ,DNA-Binding Proteins ,Repressor Proteins ,Proto-Oncogene Proteins c-kit ,Young Adult ,Genes, ras ,Leukemia, Promyelocytic, Acute ,fms-Like Tyrosine Kinase 3 ,Tandem Repeat Sequences ,Proto-Oncogene Proteins ,Mutation ,Humans ,Enhancer of Zeste Homolog 2 Protein ,Female ,Child ,Nucleophosmin ,Aged - Abstract
This study was aimed to explore whether multiple common gene mutations of leukemia synergistically involved in acute promyelocytic leukemia (APL) pathogenesis, and to investigate their relevance to clinical features, cytogenetics and molecular risk stratification. 84 specimens of admitted de novo APL patients from February 2005 to October 2010 were collected, the gene mutations of bone marrow mononuclear cells and clinical features of mutation-positive patients were analyzed by genomic DNA-PCR. The results indicated that the prevalence of mutations was 60.7% (51/84), in which the mutations with the highest incidence were found as FLT3-ITD, reaching 27.4% (23/84). Next, there were 12 cases WT1 mutation, 9 for FLT3-TKD, 7 for TET2, 5 for N-RAS, 4 for ASXL1, 2 for EZH2 mutation and 1 positive case in MLL-PTD, IDH1 and CBL mutation respectively. No mutation was found in other JAK1, DNMT3, c-Kit, NPM1, IDH2, RUNX1 and JAK2 (V617F) common leukemia-related genes. Combined analysis with clinical data demonstrated that the patients with FLT3-ITD mutation displayed higher white blood cell counts, while the patients with N-RAS mutation showed lower platelet counts. Overall survival of these patients was obviously shorten as compared with patients with wild-type. This difference between mutant and wild-type of all above mentioned cases was statistically significant (P0.05). The difference between APL with simple t (15;17) and additional abnormal karyotype was not statistically significant. It is concluded that the FLT3-ITD mutation is recurrent genetic change in APL, and together with N-RAS mutation indicates poor prognosis. Additional abnormal karyotype does not associate with prognosis of APL.
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- 2013
24. Coexistence of p210BCR-ABL and CBFβ-MYH11 fusion genes in myeloid leukemia: A report of 4 cases.
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YUAN-YUAN WANG, WEN-JING DING, FENG JIANG, ZI-XING CHEN, JIAN-NONG CEN, XIAO-FEI QI, JIAN-YING LIANG, DAN-DAN LIU, JIN-LAN PAN, and SU-NING CHEN
- Subjects
MYELOID leukemia ,HUMAN cytogenetics ,PROTO-oncogenes ,GENE rearrangement ,CHIMERIC proteins ,FLUORESCENCE in situ hybridization - Abstract
Numerous acquired molecular and cytogenetic abnormalities are strongly associated with hematological malignancies. The breakpoint cluster region-ABL proto-oncogene 1 (BCR-ABL) rearrangement leads to a p210 chimeric protein in typical chronic myeloid leukemia (CML), whereas 17-25% of patients with acute lymphocytic leukemia and 0.9-3% patients with de novo acute myeloid leukemia (AML) carry a p190
BCR-ABL fusion protein. Cases of patients with AML/CML carrying two specific primary molecular changes, BCR-ABL and core binding factor-β-myosin heavy chain 11 (CBFβ-MYH11) fusion genes have been rarely reported. The present study aimed to understand the nature and mechanism of this particular type of leukemia through case reports and literature review. A total of four patients who were diagnosed as AML/CML with BCR-ABL and CBFβ-MYH11 fusion genes in the First Affiliated Hospital of Soochow University (Suzhou, China) between January 2004 and December 2012 were examined. Morphological analysis of bone marrow cells, flow cytometry, quantitative polymerase chain reaction of p210BCR-ABL and CBFβ-MYH11 transcripts as well as cytogenetic and fluorescence in situ hybridization analyses were performed. A total of 4 patients who exhibited fusion of p210BCR-ABL and CBFβ-MYH11 were identified. A single patient (case 1) was first diagnosed CML-acute phase (AP), which progressed rapidly to CML-blast crisis (BC), and three patients (cases 2, 3 and 4) were diagnosed with AML with bone marrow eosinophilia at first presentation with no evidence of previous onset of CML. All cases achieved remission following conventional chemotherapy/hematological stem cell transplantation combined with the inhibitor of tyrosine kinase (TKI) maintenance therapy. The patients with CML carrying and expressing BCR-ABL and CBFβ-MYH11 fusion genes appeared more likely to rapidly progress to AP or BC. Therefore, the product of the CBFβ-MYH11 fusion gene may serve an important role in the transformation of CML. The co-expression of p210BCR-ABL and CBFβ-MYH11 fusion genes in myeloid leukemia may be a molecular event occurring not only during the development of CML, but also in AML. [ABSTRACT FROM AUTHOR]- Published
- 2017
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25. [Clinical and experimental studies of childhood acute myeloid leukemia with 11q23/MLL rearrangements]
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Ya-xiang, He, Yong-quan, Xue, Hong-ying, Wang, Xue-jun, Shao, Jin-lan, Pan, Jun, Xu, Nai-chao, Yang, Zheng-hua, Ji, Yi-ping, Huang, and Shao-yan, Hu
- Subjects
Male ,Adolescent ,Chromosomes, Human, Pair 11 ,Remission Induction ,Infant ,Translocation, Genetic ,Immunophenotyping ,Leukemia, Myeloid, Acute ,Treatment Outcome ,Child, Preschool ,Karyotyping ,Humans ,Female ,Child ,In Situ Hybridization, Fluorescence ,Myeloid-Lymphoid Leukemia Protein - Abstract
To explore clinical and experimental features of 28 cases of childhood acute myeloid leukemia (AML) with 11q23/MLL gene rearrangements.Karyotypes of 234 cases of de novo childhood AML were analyzed using short-term culture of bone marrow cells and R-banding. The fusion transcripts involving MLL gene and partial tandem duplication of MLL (MLL-PTD) were detected by multiple reverse transcription polymerase chain reaction (RT-PCR) assay. Two cases with 11q23 translocation by karyotypic analysis but with negative result of multiple RT-PCR were studied with MLL-dual-color fluorescence in situ hybridization (D-FISH).R-banding karyotypic analysis has revealed 20 cases with 11q23 translocation (14 cases with M5, 4 cases with M4, 2 cases with M2), including 12 cases with t(9;11)(p22;q23), 3 cases with t(1;11)(q21;q23), 2 cases with t(6;11)(q27;q23), 1 case with t(11;19)(q23;p13), 1 with t(5;11)(q31;q23), and 1 with t(X;11)(q24;q23). Eighteen cases with 11q23 translocation having fusion transcripts involving MLL genes were confirmed with multiple RT-PCR; 2 cases showed negative results, but they were confirmed to have MLL rearrangements by D-FISH. MLL-PTD was also detected in 8 cases (4 cases M5, 2 cases M4, M2 and M6, one case each) from the other childhood AML cases. The total incidence of 11q23/MLL gene rearrangements was 11.97% (28/234), and most of patients(85.7%, 24/28) were M4/M5. The complete remission (CR) rate after treatment for the 28 cases with MLL rearrangements was 53.8%, the difference was significant by statistics (P0.05) compared with 90.5% for the control group (M4/M5 childhood AML with other karyotypic abnormalities or normal karyotype). Of them, 2 cases receiving intensive chemotherapy survived for 81 and 66 months, respectively, 4 cases receiving allogeneic stem cell transplantation survived for 21, 20, 16 and 11 months, respectively, and are still alive with CR. The medium survival (MS) time for 28 cases with 11q23/MLL rearrangements was 11 months, whereas the MS for control group was 15 months. The difference was not statistically significant(P0.05).The 11q23/MLL rearrangements is highly correlated with the occurrence of monocytic leukemia (M4 and M5). The 11q23 translocation and MLL-PTD are mutually exclusive, though both are indicative of poor prognosis. Intensive chemotherapy and allogeneic stem cell transplantation may ameliorate the clinical outcome. Multiple RT-PCR combined with karyotypic analysis and D-FISH are useful for screening the 11q23/MLL rearrangements in childhood AML.
- Published
- 2012
26. [Expression of SET-NUP214 fusion gene in patients with T-cell acute lymphoblastic leukemia and its clinical significance]
- Author
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Hai-Ping, Dai, Qian, Wang, Li-Li, Wu, Na-Na, Ping, Chun-Xiao, Wu, Jun-Dan, Xie, Jin-Lan, Pan, Yong-Quan, Xue, De-Pei, Wu, and Su-Ning, Chen
- Subjects
Oncogene Proteins, Fusion ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression ,Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ,DNA-Binding Proteins ,Nuclear Pore Complex Proteins ,Repressor Proteins ,Mutation ,Humans ,Histone Chaperones ,Chromosome Deletion ,Receptor, Notch1 ,Carrier Proteins ,Chromosomes, Human, Pair 9 ,Transcription Factors - Abstract
This study was aimed to investigate the occurrence and clinical significance of the SET-NUP214 fusion gene in patients with T-cell acute lymphoblastic leukemia (T-ALL), analyse clinical and biological characteristics in this disease. RT-PCR was used to detect the expression of SET-NUP214 fusion gene in 58 T-ALL cases. Interphase FISH and Array-CGH were used to detect the deletion of 9q34. Direct sequencing was applied to detect mutations of PHF6 and NOTCH1. The results showed that 6 out of 58 T-ALL cases (10.3%) were detected to have the SET-NUP214 fusion gene by RT-PCR. Besides T-lineage antigens, expression of CD13 and(or) CD33 were detected in all the 6 cases. Deletions of 9q34 were detected in 4 out of the 6 patients by FISH. Array-CGH results of 3 SET-NUP214 positive T-ALL patients confirmed that this fusion gene was resulted from a cryptic deletion of 9q34.11q34.13. PHF6 and NOTCH1 gene mutations were found in 4 and 5 out of 6 SET-NUP214 positive T-ALL patients, respectively. It is concluded that SET-NUP214 fusion gene is often resulted from del(9)(q34). PHF6 and NOTCH1 mutations may be potential leukemogenic event in SET-NUP214 fusion gene.
- Published
- 2012
27. [Clinical and experimental study of a multiple myeloma case with low hypodiploidy]
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Shu-xiao, Bai, Jin-lan, Pan, Yong-quan, Xue, Su-ning, Chen, Ya-fang, Wu, Yong, Wang, Jun, Zhang, and Juan, Shen
- Subjects
Adult ,Gene Rearrangement ,Cytogenetics ,Abnormal Karyotype ,Humans ,Female ,Multiple Myeloma - Abstract
To report the clinical and laboratory characterization of a case of multiple myeloma with low hypodiploid complex karyotyptic abnormalities.Cytogenetic examination of bone marrow performed by 24 h culture method. R-banding technique was used to analyze the karyotype. Interphase fluorescence in situ hybridization (FISH) was performed using chromosome probes such as 13q14, p53, Rb1, 1q21 and IgH/CCND1. The DNA content was detected by flow cytometry.Chromosome analysis revealed complex chromosomal rearrangement. Five cells had a low hypodiploid karyotype with 35 chromosomes. Three cells had the duplication of the low hypodiploid karyotype. Four cells had a normal karyotype. Monosomy 1, 13, 14, 17 and a mark chromosome 1 derived from chromosome 11 resulting in the amplication of CCND1 gene were confirmed by interphase FISH. Flow cytometric analysis displayed a low hypodiploid peak with the DNA index of 0.8426.These results indicated that the low hypodiploidy is a rare abnormality in multiple myeloma. Interphase FISH is a reliable method for detecting molecular abnormalities in multiple myeloma.
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- 2012
28. [Fluorescence in situ hybridization study of acute myeloid leukemia with cryptic chromosome rearrangements]
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Shu-xiao, Bai, Yong-quan, Xue, Su-ning, Chen, Jin-lan, Pan, Ya-fang, Wu, Juan, Shen, Yong, Wang, and Jun, Zhang
- Subjects
Adult ,Male ,Leukemia, Myeloid, Acute ,Young Adult ,Adolescent ,Oncogene Proteins, Fusion ,Karyotyping ,Humans ,Female ,Middle Aged ,In Situ Hybridization, Fluorescence ,Translocation, Genetic ,Chromosome Banding - Abstract
To detect specific chromosome rearrangements in acute myeloid leukemia (AML) using interphase-fluorescence in situ hybridization (FISH).All cases were studied by R-band karyotypic analysis using direct method and/or short-term culture for chromosomes preparation. Interphase-FISH was performed in 108 cases of AML with M5, M4, M2, M3 subtypes including 103 cases with normal karyotypes, 4 cases with chromosomal abnormalities other than specific chromosomal rearrangements using chromosome translocation probe such as AML1/ETO, PML/RARα, CBFβ/MYH11 and MLL.Of 38 cases of M2-AML without t(8;21) on conventional cytogenetics(CC) analysis, 4 cases showed positivity for AML1/ETO fusion transcript, which included 2 cases with typical signal model and 2 with insertion. Of 9 cases of M3-AML without t(15;17) on CC analysis, 6 showed positivity for PML/RARα fusion transcript including 2 with typical signal model, 3 with insertion, one without PML/RARα rearrangement on reverse transcription-PCR and FISH assay using PML/RARα probe. FISH assay using the RARα dual color, break-apart rearrangement probe indicated a partial deletion of RARα. Of 23 cases with M4 or M4EO-AML without inv(16) on CC analysis, 3 showed positivity for CBFβ/MYH11 fusion transcript. Of 38 cases without 11q23 translocation on CC analysis, all cases were negative for MLL rearrangement.Interphase-FISH can detect specific chromosome rearrangements such as AML1/ETO, PML/RARα or CBFβ/MYH11 in some AML cases with normal karyotype, though it seemed less useful for the detection of MLL rearrangement.
- Published
- 2011
29. [Report of ten cases of hematologic malignancies with idic(20q-) and literature review]
- Author
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Yong-sheng, Han, Yong-quan, Xue, Tian-yu, Li, Jun, Zhang, Su-ning, Chen, Jin-lan, Pan, Ya-fang, Wu, Yong, Wang, and Juan, Shen
- Subjects
Adult ,Chromosome Aberrations ,Isochromosomes ,Male ,Myelodysplastic Syndromes ,Chromosomes, Human, Pair 20 ,Humans ,Female ,Chromosome Deletion ,Middle Aged ,In Situ Hybridization, Fluorescence ,Aged - Abstract
To analyze the clinical and molecular cytogenetic features of hematologic malignancies with idic(20q-).The clinical data of 10 patients with idic (20q-) were analyzed. Karyotyping analysis was carried out with R banding technique. A CEP20 probe was used to perform single-color fluorescence in situ hybridization (FISH). A subtelomeric probe for 20q and a locus-specific probe for 20q12 were used to perform dual-color FISH. The literatures of hematologic malignancies with idic(20q-) were reviewed.Of the 10 cases, 2 were diagnosed as acute erythroid leukemia, 1 primary myelofibrosis, 3 myelodysplastic syndromes (MDS) and 4 highly suspected (HS-MDS). Karyotype analysis showed that one of the normal chromosome 20 allele was substituted by one or two metacentric isochromosomes smaller than the normal one in all 10 cases. It was confirmed to be der(20)del(20)(q11q13)idic(20)(p11), i.e., idic(20q-) by FISH assay. Partial cells in 2 of the 10 cases had 20q- as the sole karyotypic anomaly.Idic(20q-) results from a pre-existing del(20q) and is strongly associated with MDS and acute erythroid leukemia. Idic(20q-) as a recurrent cytogenetic abnormality is helpful for diagnosing HS-MDS in patients with cytopenia but only slight or absent dysplasia.
- Published
- 2011
30. [The different signal patterns of two FISH probes in the FISH detection of Ph-positive leukemia and their clinical significance]
- Author
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Hui, Jiang, Yong-quan, Xue, Jin-lan, Pan, Jun, Zhang, Hai-ping, Dai, Ya-fang, Wu, Yong, Wang, Juan, Shen, and Su-ning, Chen
- Subjects
Case-Control Studies ,Karyotyping ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Proto-Oncogene Proteins c-bcr ,Humans ,Chromosomes, Human, Pair 9 ,In Situ Hybridization, Fluorescence - Abstract
To compare the signal patterns of dual color extra-signal BCR/ABL probe (ES-FISH) and dual color dual fusion BCR/ABL probe (D-FISH) in the fluorescence in situ hybridization (FISH) detection of Ph-positive leukemia, and to explore their diagnostic value.ES-FISH probe and D-FISH probe were used, respectively, to detect the BCR/ABL fusion gene in 74 cases with typical t(9;22)(q34;q11) and 37 cases with variant t(9;22)(q34;q11) translocation or complex karyotypic abnormalities containing Ph translocation.The BCR/ABL fusion gene in all cases with typical t(9;22)(q34;q11) could be detected by both FISH probes. D-FISH had a signal pattern of 1O1G2F, while ES-FISH showed a signal pattern of 2O1G1F. ES-FISH enables the minor breakpoint cluster region to be identified in 9 cases (12.2% ) of Ph-positive leukemia, whereas D-FISH could not differentiate the minor breakpoint cluster region from major breakpoint cluster region. D-FISH could distinguish simple ABL gene deletion from simultaneous deletion of the ABL and BCR genes in 8 cases (10.8%) of Ph-positive leukemia patients, but ES-FISH could not. For variant Ph translocation or complex karyotypic abnormalities containing Ph translocation, each FISH probe showed four or six types of signal pattern, most of which were atypical. The exact interpretation was dependent on conventional karyotypic analysis and FISH on metaphases.ES-FISH and D-FISH probes displayed different signal patterns in Ph-positive leukemia due to their differences in size and covered regions. ES-FISH and D-FISH probes may be selected as better probe for Ph-positive acute lymphocytic leukemia and Ph-positive chronic myeloid leukemia, respectively. When imatinib was used for treatment, there was no preference between ES-FISH and D-FISH probe, because major breakpoint cluster region, minor breakpoint cluster region and partial sequence deletion of derivative chromosome 9, would not affect the prognosis of Ph-positive leukemia. However, considering that ES-FISH probe has a better cost-performance than D-FISH probe does, it is recommended as first choice.
- Published
- 2010
31. [A clinical and laboratory study of TCF3-PBX1 positive adult acute lymphoblastic leukemia.]
- Author
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Ji-Fu, Zheng, Hui-Ying, Qiu, Jin-Lan, Pan, Jian-Nong, Cen, Ya-Fang, Wu, Jun, Zhang, De-Pei, Wu, and Yong-Quan, Xue
- Subjects
Adult ,Oncogene Proteins, Fusion ,Chromosomes, Human, Pair 1 ,Humans ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,In Situ Hybridization, Fluorescence ,Translocation, Genetic - Abstract
To explore the morphology, immunophenotype, cytogenetics and clinical features of TCF3-PBX1 fusion gene positive adult acute lymphoblastic leukemia (ALL).R banding was used to analyze conventional cytogenetics (CC), interphase fluorescence in situ hybridization (iFISH) and RT-PCR to detect the TCF3-PBX1 fusion gene, and flow cytometry to immunophenotype. The clinical and laboratory features and long-term follow-up of the patients were analyzed.The incidence of 19 TCF3-PBX1-positive adult ALL was 3.13% of total ALL patients. Of them, 12 and 7 cases were diagnosed as L(1) and L(2) morphology respectively; 7 cases with balanced translocation of chromosome 1 and 19; 10 with der(19) t(1;19) formed from unbalanced translocation and 2 with normal karyotypes. TCF3-PBX1 fusion gene was detected by RT-PCR in 9 cases, and by iFISH in 17. 16 cases were B-phenotype and the other 2 T-phenotype; 17 cases had lymph node, spleen or liver infiltration. Of 18 patients received chemotherapy, 17 (94.7%) achieved complete remission (CR); the median relapse-free survival (RFS) and median overall survival was 3.2 months and 7.2 months, respectively.TCF3-PBX1-positive adult ALL had unique clinical and pathological features with high remission rate, high relapse rate and short survival time and should be considered to receive intensified treatment strategies. iFISH combined with CC and RT-PCR can increase the detection rate of t(1;19)/TCF3-PBX1 fusion gene.
- Published
- 2010
32. [Establishment and characterization of a new human myeloid leukemia cell line SH-2]
- Author
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Hui-Ying, Qiu, Yong-Quan, Xue, Jun, Zhang, Hai-Ping, Dai, Jin-Lan, Pan, Ya-Fang, Wu, Su-Ning, Chen, Yong, Wang, Juan, Shen, Ai-Ning, Sun, and De-Pei, Wu
- Subjects
Adult ,Male ,Leukemia, Myeloid, Acute ,Cell Line, Tumor ,Cell Culture Techniques ,Humans ,Cell Separation ,Immunophenotyping - Abstract
To establish and characterize a novel human myeloid leukemia cell line SH-2.Bone marrow mononuclear cells (BMMNC) isolated from a AML-M2 patient, who failed to obtain complete remission after chemotherapy and allogenic bone marrow transplantation were passed in a long term IMDM culture medium supplemented with 20% fetal calf serum. Stromal cells were retained and rh-IL-3 was added in the culture system. A new human myeloid leukemia cell line SH-2 was successfully established with a cytogenetic characteristics of a loss of Y chromosome (-Y), a derivative chromosome 16 resulting from unbalanced translocation between chromosome 16 and 17, monosome 17, trisomy 19 and p53 alteration. Various methods were employed to characterize SH-2 cell line.SH-2 cells has been maintained without cytokine and stromal cells for more than 3 years without EB virus and mycoplasma contamination. SH-2 cells had the basically same morphological, immunophenotypic and cytogenetic features as the patient's leukemia cells did, such as myeloid morphology, an immunophenotype of CD13+, CD33+, CD56+, CD16/56+ and a hypodiploid karyotype of 45, X, -Y, der(16)t(16;17)(q24;ql2), -17, +19, which were gradually decreased and replaced by the near-tetraploid cells with a karyotype of 73-102(80), XX, -Y, -Y, del (q131)x2, der(16)t(16;17)(q24;q12)x2, -17, -17, +19, +19. FISH and multiple FISH delineated all the abnormalities and revealed a loss of one p53 allele due to monosomy 17. DNA direct sequencing detected a point mutation of CAG to CAT at codon 576 of exon 5 in another p53 allele. RT-PCR showed that SH-2 cells expressed apoptosis-related genes (bcl-2, Fas, GST-pi and p2) rather than MDR-related genes. Short tandem repeat PCR provided powerful evidence for the derivation of SH-2 cell line from the patient's leukemia cells. SH-2 cells had certain colony formation and tumorigenic capacities in nude and SCID mice.SH-2 is a new myeloid leukemia cell line with a unique biology background, and will provide a useful tool for leukemia research.
- Published
- 2009
33. [Fluorescence in situ hybridization studies on a myeloid leukemia patient with ins(8;21)(q22;q22.1q22.3)]
- Author
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Ya-fang, Wu, Yong-quan, Xue, Shu-xiao, Bai, Jun, Zhang, Li, Yao, Yong, Wang, Hui-ying, Qiu, Juan, Shen, Jin-lan, Pan, and Qin-fen, Ma
- Subjects
Chromosomes, Human, Pair 15 ,Leukemia, Myeloid ,Karyotyping ,Core Binding Factor Alpha 2 Subunit ,Humans ,Female ,Chromosomes, Human, Pair 19 ,In Situ Hybridization, Fluorescence ,Translocation, Genetic ,Chromosome Banding ,Chromosomes, Human, Pair 8 - Abstract
To report a case of acute myeloid leukemia (AML) with the insertion (8;21)(q22;q22.1q22.3). A 33-year-old Chinese woman was referred to our hospital. Hematologic data showed WBC 42.7 x 10(9)/L with monocytosis (monocyte counts 7.296 x 10(9)/L). Bone marrow aspirate was hypercellular with 4.5% monoblasts and 7.5% promonocytes. At first she was diagnosed with chronic myelomonocytic leukemia (CMML) according to the FAB criteria. Initially the patient received supportive care only, but her general condition rapidly became worse three months later. The monoblasts and promonocytes in the bone marrow rose to 20.5%. After two cycles of combined chemotherapy she obtained complete remission.Chromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was carried out by R-banding technique. Three fluorescence in situ hybridization (FISH) analyses were performed using AML1-ETO dual color, dual fusion probe, whole chromosome painting 8 and 21 probes, and cen-8 and Tel 21qter probes, respectively. Reverse transcription polymerase chain reaction (RT-PCR) assay for detecting the AML1-ETO fusion transcript was also performed.Conventional cytogenetic analysis showed a karyotype of 46,XX,ins(8;21) (q22;q22.1q22.3)[7]/46,XX[3]. FISH tests confirmed the insertion. RT-PCR analysis detected the AML1-ETO fusion transcript.We consider that this patient should be rediagnosed as acute myeloid leukemia according to the criteria proposed by World Health Organization (WHO) and that FISH and RT-PCR play an important role in verification of the ins(8;21).
- Published
- 2009
34. [Osteoblasts from patients with myelodysplastic syndrome express multiple cytokines and support hematopoietic progenitor cell survival in vitro]
- Author
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Wen-Ming, Chen, Zi-Xing, Chen, Jian-Nong, Cen, Jun, He, Xue-Li, Jiao, Jin-Lan, Pan, Qiao-Cheng, Qiu, Lan, Dai, and Dan-Dan, Liu
- Subjects
Stem Cell Factor ,Osteoblasts ,Granulocyte-Macrophage Progenitor Cells ,Interleukin-6 ,Myelodysplastic Syndromes ,Cytokines ,Granulocyte-Macrophage Colony-Stimulating Factor ,Humans ,RNA, Messenger ,Hematopoietic Stem Cells - Abstract
This study was aimed to investigate the biological characteristics of osteoblasts from patients with myelodysplastic syndrome (MDS) and their supportive capacity for hematopoiesis in vitro. A two-dimensional culture system was constructed by using osteoblasts derived from human marrow mesenchymal stem cells (MSC); MSCs were isolated from bone marrow of MDS patients and normal individuals and were cultured; the third passage of MSCs were induced into osteoblasts which were treated with mitomycin C and confluenced into a feeder layer. Ficolled bone marrow mononuclear cells were obtained from normal individuals and seeded into the two-dimensional culture system to culture in vitro without exogenous cytokines. By using colony-forming assay, the ability of the two-dimensional system to culture HPCs was observed. The cytokine expression of osteoblasts from MDS patient bone marrows in mRNA level was detected by RT-PCR and was compared with human osteoblast cell line hFOB1.19. The results showed that the osteoblasts from MDS patients could support short-term survival of GM-CFC in condition without exogenous cytokines, that is, osteoblasts played a crucial role in regulation of HPC growth. The results of RT-PCR clearly demonstrated that the osteoblast cell line hFOB1.19 expressed SCF, IL-6, SDF-1alpha, G-CSF and GM-CSF. The same expression patterns of above cytokines were also seen in osteoblasts derived from BM-MSCs of MDS patients and normal individuals, but these cells did not express GM-CSF. It is concluded that the biological characteristics of osteoblasts from bone marrow of MDS patients are generally not different from those of osteoblasts from normal bone marrow. Both of them can support GM -CFC to form colonies in vitro, it may be associated with expressing important related cytokines by osteoblasts.
- Published
- 2008
35. [Multiplex fluorescence in situ hybridization for detecting complex chromosomal aberrations in chronic myeloid leukemia in blast crisis]
- Author
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Yu, Zhu, Jian-Yong, Li, Wei, Xu, Hai-Rong, Qiu, Li-Juan, Chen, Jin-Lan, Pan, Yun-Feng, Shen, and Yong-Quan, Xue
- Subjects
Adult ,Chromosome Aberrations ,Male ,Karyotyping ,Humans ,Female ,Leukemia, Myeloid, Accelerated Phase ,Middle Aged ,Blast Crisis ,In Situ Hybridization, Fluorescence - Abstract
To investigate the value of multiplex fluorescence in situ hybridization (M-FISH) for the detection of complex chromosomal abnormalities (CCA) of chronic myeloid leukemia in blast crisis (CML-BC).M-FISH was used to study 26 cases of CML-BC with CCA assayed by conventional cytogenetics (CC).Sixty-nine kinds of structural rearrangements were detected by M-FISH besides typical t (9;22) translocation, among them only 10 were balanced ones and 59 unbalanced ones including 1 insertion, 6 deletions, 52 translocations and derivative chromosomes. In addition, 23 numerical abnormalities were detected. All chromosomes were involved in CML-BC, and chromosomes 17, 2, 8, 16 involvements were the most frequent. M-FISH failed to find out the abnormal clone in 1 case, discovered CCA clones that were missed CC in 6 cases. Clarified 16 kinds of aberrations which could not be identified CC and corrected 5 aberrations made wrong description by CC. Thirty-five kinds of translocations were found by M-FISH which were missed by CC. The aberrations of der (9) t (16; 6; 9; 22) and der (18) t (16; 18; 19) we found were reported in the literature for the first time.M-FISH can refine CCA in CML-BC, find out or correct the missed or misidentified abnormalities by CC. The frequent secondary chromosomal abnormalities in CML-BC with CCA are different from that in CML.
- Published
- 2007
36. [Analysis of complex chromosomal aberrations in patients with myelodysplastic syndromes using multiplex fluorescence in situ hybridization combined with whole chromosome painting]
- Author
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Li-juan, Chen, Jian-yong, Li, Bing, Xiao, Yu, Zhu, Qiong, Liu, Jin-lan, Pan, Hai-rong, Qiu, Lei, Fan, Su-jiang, Zhang, Rui-nan, Lu, Wei, Xu, and Yong-quan, Xue
- Subjects
Adult ,Chromosome Aberrations ,Male ,Adolescent ,Middle Aged ,Chromosome Banding ,Chromosome Painting ,Young Adult ,Karyotyping ,Myelodysplastic Syndromes ,Humans ,Female ,In Situ Hybridization, Fluorescence ,Aged ,Chromosomes, Human, Pair 17 - Abstract
To explore the value of multiplex fluorescence in situ hybridization (M-FISH) in combination with whole chromosome painting (WCP) in the detection of complex chromosomal aberrations (CCAs) in myelodysplastic syndromes (MDS).M-FISH was used in seven MDS patients with R-banding CCAs to refine the complex chromosomal rearrangements, and to identify cryptic translocations and characterization of marker chromosomes. Dual-color WCP procedures were further performed in 7 cases to confirm some rearrangements detected by M-FISH.M-FISH confirmed all results of R-banding. The composition and origin of 6 kinds of marker chromosomes, 9 kinds of chromosomes with additional material undetermined and 5 kinds of derivative chromosomes undefined by conventional cytogenetics (CC) were defined after M-FISH analysis; four kinds of cryptic translocations overlooked by CC were found on derivative chromosomes and previously normal appearing chromosomes. In addition, M-FISH revealed some nonrandom aberrations: aberrations involving chromosome 17 and -5/5q- were the two most frequent aberrations. Some misclassified and missed chromosomal aberrations by M-FISH were corrected by WCP.M-FISH is a powerful molecular cytogenetic tool in clarification of CCAs. Complementary WCP helps us to identify misclassified and missed chromosomal aberrations by M-FISH. CC in combination with molecular cytogenetic techniques, such as M-FISH and WCP, can unravel complex chromosomal aberrations more precisely.
- Published
- 2007
37. [Study on molecular cytogenetic abnormalities in multiple myeloma]
- Author
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Shu-Yan, Liu, Jian-Yong, Li, Li-Juan, Chen, Jin-Wen, Huang, Jin-Lan, Pan, Hai-Rong, Qiu, Yun-Feng, Shen, Wei, Xu, and Yong-Quan, Xue
- Subjects
Chromosome Aberrations ,Gene Rearrangement ,Male ,Plasma Cells ,Humans ,Female ,Middle Aged ,Immunoglobulin Heavy Chains ,Multiple Myeloma ,Gene Deletion ,In Situ Hybridization, Fluorescence ,Aged - Abstract
To explore the molecular cytogenetic abnormalities in multiple myeloma (MM).Bone marrow plasma cells from 23 previously untreated MM patients were purified by CD138 McAb magnetic cell sorting system, and a panel of probes for interphase fluorescence in situ hybridization were used to detect the 13q14 deletion, p53 deletion and IgH gene translocation in the sorted MM cells.Among 23 MM patients, 13q14 deletion was observed in 10 (43.5%) cases, with the positive rate of 13q14 deleted cells ranged from 79% to 96%; 14q32 translocation was observed in 11 (47.8%) cases; 13q14 deletion and 14q32 translocation were simultaneously observed in 7 (30.4%) cases; and p53 deletion was observed in none of the 23 cases.The frequency of 13q14 deletion and IgH gene translocation in multiple myeloma are high; and the relationship between 13q14 deletion, IgH gene translocation and prognosis is worth further investigating.
- Published
- 2007
38. [Analysis of NPM1 gene mutations in acute myeloid leukemia]
- Author
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Ling-zhi, Yan, Su-ning, Chen, Jian-ying, Liang, Yu-feng, Feng, Jian-nong, Cen, Jun, He, Wei-rong, Chang, Zi-ling, Zhu, Jin-lan, Pan, Ya-fang, Wu, Yong-quan, Xue, and De-pei, Wu
- Subjects
Adult ,Male ,Leukemia, Myeloid, Acute ,Adolescent ,DNA Mutational Analysis ,Mutation ,Humans ,Nuclear Proteins ,Female ,Exons ,Middle Aged ,Nucleophosmin ,Aged - Abstract
To evaluate the prevalence of nucleophosmin (NPM1) gene exon 12 mutations in adults with acute myeloid leukemia (AML) and its clinical characteristics.Genomic DNAs from 101 AML adults were screened by PCR and sequencing or capillary electrophoresis (CE) for NPMI mutations.NPM1 exon 12 mutations were present in 31.7% of the overall cohort, including 1/1 (100%) of M0, 9/17(52.9%) of M1 , 7/25 (28.0%) of M2, 0/23(0%) of M3, 2/7 (28.6%) of M4 and 13/25 (52.0% ) of M5. NPM1 gene mutations were more prevalent in patients with normal karyotype (27/59, 45.8%) compared with that in those with karyotypic abnormalities (5/42, 11.9% ) (P0.001). NPM1 mutant cases were significantly associated with old age (P0.05), high peripheral white cell count (P0.05) and low expression of CD34 (P0.05) and CD17 (P0.05). Sequence analysis of these NPM1 mutant cases revealed 5 known mutations (type A, B, D, N(M), and P(M)) and 1 novel variant (named as type S).NPM1 exon 12 mutations occur with a considerable percentage in AML patients with normal karyotype, M1/M5 subtype and older age, and are associated with higher peripheral white cell count and lower expression of CD34 and CD117.
- Published
- 2007
39. [High tumorigenicity of human acute monocytic leukemic cell Line SHI-1 in nude mice and its mechanism]
- Author
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Su-Ning, Chen, Yong-Quan, Xue, Jin-Lan, Pan, Ya-Fang, Wu, Yong, Wang, and Jian-Nong, Cen
- Subjects
Vascular Endothelial Growth Factor A ,Disease Models, Animal ,Mice ,Mice, Inbred BALB C ,Matrix Metalloproteinase 9 ,Leukemia, Monocytic, Acute ,Tumor Cells, Cultured ,Animals ,Humans ,Matrix Metalloproteinase 2 ,Mice, Nude ,Genes, p53 ,Neoplasm Transplantation - Abstract
This study was purposed to explore the tumorigenicity of a novel human monocytic leukemic cell line SHI-1 in nude mice and its mechinism. The tumorigenicity in mice was evaluated in sixteen nude mice subcutaneously injected with the SHI-1 cell line. The tumor specimen was studied by the conventional pathologic examination. The mononuclear cells (MNC) of the tumor was assayed by RHG banding, the transcription of MLL-AF6 fusion gene and the VEGF gene was detected by RT-PCR. Gelatin zymography method was used to study the expression of MMP-9 and MMP-2 in the supernatant of the SHI-1 cell line. Matrigel invasion assay was employed for the study of migration of the SHI-1 cell in vitro. The results showed that the tumor masses were found in all sixteen mude mice after subcutaneous injection of SHI-1 cells, the tumor mass was mainly composed of leukemia cells, the transcription of MLL-AF6 fusion gene and VEGF gene was proved by RT-PCR analysis, the expressions of MMP-2 and MMP-9 in the serum-free culture supernatant of the SHI-1 cell line were significantly higher than those in U937, K562, and NB4 cell lines. The SHI-1 cell line exhibited significantly higher in vitro invasiveness than other leukemia cell lines, the blocking antibody of MMP-2 could inhibit the migration of the SHI-1 cell line significantly. It is concluded that the SHI-1 cell line presents higher tumorigenicity in nude mice than other leukemia cell line and the mechanism is associated with p53 gene alteration, high transcription level of VEGF gene, high expression level of MMP, and significantly higher invasiveness.
- Published
- 2007
40. [Clinical and experimental retrospective analysis on acute leukemia with trisomy 4 cell]
- Author
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Jin-lan, Pan, Yong-quan, Xue, Hui-ying, Qiu, Jun, Zhang, Ya-fang, Wu, Yong, Wang, Juan, Shen, and Yong-jin, Zhu
- Subjects
Adult ,Male ,Young Adult ,Leukemia ,Karyotyping ,Acute Disease ,Humans ,Female ,Trisomy ,Chromosomes, Human, Pair 4 ,Middle Aged ,In Situ Hybridization, Fluorescence ,Aged - Abstract
To explore the clinical and experimental features of acute leukemia (AL) with trisomy 4.A retrospective analysis on the clinical and laboratory data of 21 cases of AL with trisomy 4 was performed. Chromosomes were prepared using direct method and/or short-term (24 h) cultures of bone marrow cells. Karyotypic analysis was carried out by using R-banding technique. Thirteen cases were studied by interphase fluorescence in situ hybridization (FISH) by using a chromosome 4-specific alpha -satellite DNA probe labeled by spectrum Green to ascertain the presence of a clone with trisomy 4. Five cases with t (8; 21) revealed by karyotypic analysis were detected by dual-color FISH using t (8; 21) translocation probe to confirm the AML1/ETO rearrangement.All the patients with AL and trisomy 4 were with de novo AL except two cases with secondary AL. M2 was the most frequent Franch-American-British(FAB) subtype in this series (9/21 cases). The initial leukocyte count more than 10x 10(9)/L was seen in 16 cases. An enlargement of liver, spleen and/or lymph nodes in varying degrees was found in 15 cases. Among 15 cases received immunophenotypic analysis, 11 cases showed CD34 positivity and 6 cases co-expressed myeloid and lymphocyte antigens. Karyotypic analysis disclosed clonal trisomy 4 in 18 cases and one cell with +4 in 3 cases. Isolated trisomy 4 was found in 7 cases, while 14 cases had other abnormalities besides trisomy 4 among which t (8; 21) was found in 8 cases. Dual-color FISH confirmed that all 13 cases including 3 cases having one cell with +4 on karyotypic analysis had clonal trisomy 4. Dual-color FISH confirmed that all 5 cases with t (8; 21) had AML1/ETO rearrangement.AL patients with trisomy 4 have unique clinical and experimental features and a poor prognosis.
- Published
- 2007
41. [Study of trisomy 22 and inversion 16 in acute myeloid leukemia]
- Author
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Hui-fen, Zhou, Jian-yong, Li, Jin-lan, Pan, Hai-rong, Qiu, Li-juan, Chen, Jie-ying, Hu, Yun-feng, Shen, Wei, Xu, and Yong-quan, Xue
- Subjects
Adult ,Male ,Leukemia, Myeloid, Acute ,Adolescent ,Chromosomes, Human, Pair 22 ,Chromosome Inversion ,Humans ,Female ,Trisomy ,Middle Aged ,Chromosomes, Human, Pair 16 ,In Situ Hybridization, Fluorescence ,Aged - Abstract
To explore the value of trisomy 22 ( +22) in the diagnosis of inv(16) acute myeloid leukemia (AML).Interphase fluorescence in situ hybridization (FISH) was performed in 18 AML patients with +22. The probe was two-color break apart probe for CBFbeta with SpectrumRed on the centromeric side and SpectrumGreen on the telomeric side. The FISH results were compared with that of R-banding conventional cytogenetics (CC). Multiplex FISH (M-FISH) was used to analyze the relationship of +22 and inv(16).CC revealed inv(16) in none of the 18 AML, with +22, but FISH revealed inv (16) in 11 of them and del( 16) (q22) in one. As CC results, 9 of the 11 cases were sole +22, one complicated with trisomy 8, and one del(16) (q22). Four patients with +22 and inv(16) were analyzed by M-FISH and revealed +22 only.+22 can be regarded as an important marker for the diagnosis of inv(16) AML.
- Published
- 2007
42. [The clinical implication of JAK2 mutation expression in patients with myeloproliferative disorders]
- Author
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Hai-rong, Fei, Ri, Zhang, Su-ning, Chen, Jin-lan, Pan, Jian-nong, Cen, and Yong-quan, Xue
- Subjects
Adult ,Aged, 80 and over ,Male ,Myeloproliferative Disorders ,Adolescent ,Base Sequence ,DNA Mutational Analysis ,Age Factors ,Janus Kinase 2 ,Middle Aged ,Polymerase Chain Reaction ,Gene Frequency ,Mutation ,Humans ,Female ,Child ,Aged - Abstract
To investigate the frequency and clinical implication of JAK2V617F mutation in Chinese patients with myeloproliferative disorders (MPD).Genomic DNA from bone marrow or blood mononuclear cells of 137 cases of MPD was screened with allele-specific polymerase chain reaction (PCR) and JAK2V617F mutation was detected with gel electrophoresis. There were 57 cases with polycythemia vera (PV), 68 with essential thrombocythemia (ET), 12 with idiopathic myelofibrosis (IMF).JAK2V617F mutation was detected in 42 (73.7%) of the 57 patients with PV, 40 (58.8%) of the 68 with ET and 8 (66.7%) of the 12 with IMF. Sequence analysis of PCR products from selected patients confirmed the coexistence of both mutant and wild-type alleles. A higher prevalence was observed in elderly patients with MPD (P0.05). Cytogenetic analysis was performed in 115 of the 137 patients. Among the 108 patients with normal karyotype, JAK2V617F mutation was detected in 74 patients (68.5%) as compared with 5 of the 7 patients with karyotypic abnormalities (71.4%).JAK2V617F mutation occurs in a significant percentage of Chinese patients with the myeloproliferative disorders. There is a higher prevalence in elderly patients.
- Published
- 2007
43. [Cytogenetic analysis in patients with polycythemia vera]
- Author
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Li-Min, Duan, Jian-Yong, Li, Jin-Lan, Pan, Hai-Rong, Qiu, Su-Jiang, Zhang, Ya-Fang, Wu, Wei, Xu, and Yong-Quan, Xue
- Subjects
Adult ,Male ,Cytogenetic Analysis ,Humans ,Female ,Trisomy ,Middle Aged ,Chromosomes, Human, Pair 9 ,Polycythemia Vera ,Gene Deletion ,In Situ Hybridization, Fluorescence ,Aged ,Chromosomes, Human, Pair 8 - Abstract
In order to evaluate the incidence of chromosomal abnormalities in patients with polycythemia vera (PV) accurately and to investigate the value of fluorescence in situ hybridization (FISH) technique in the detection of trisomies 8 and 9, conventional cytogenetics (CC) technique was used to detect karyotype and interphase FISH was used to detect trisomies 8 and 9 in 50 newly diagnosed PV and 8 normal individuals. The results showed that out of 50 cases, the 3 cases had chromosome karyotype abnormality, including trisomy 8, deletion of chromosome Y and inversion of chromosome 11 by CC technique. FISH method detected two cases of trisomy 8, including one case confirmed by CC technique, and one case of trisomy 9 neglected by CC technique. It is concluded that the incidence of chromosomal abnormalities in patients with PV is rare, and the incidence of trisomy 8 and trisomy 9 found in this study are relatively lower than that have been reported which may be related to the limited number of samples. Interphase FISH, as an important complement to CC, is a useful method for the detection of trisomies 8 and 9.
- Published
- 2007
44. [Karyotype analysis in 119 patients with chronic myeloid leukemia at blast crisis]
- Author
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Yu, Zhu, Jian-Yong, Li, Jin-Lan, Pan, Wei, Wu, Hai-Rong, Qiu, Ya-Fang, Wu, Hui, Yang, Jian-Fu, Zhang, Li-Juan, Chen, and Yong-Quan, Xue
- Subjects
Adult ,Male ,Karyotyping ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Humans ,Female ,Chromosome Deletion ,Blast Crisis ,Chromosomes, Human, Pair 9 - Abstract
To investigate the karyotype of chronic myeloid leukemia in blast crisis (CML-BC), karyotype analysis was performed with R-banding technique in 119 patients with CML-BC. Dual fusion fluorescence in situ hybridization (FISH) was used to detect derivative chromosome 9 deletions in randomly selected 28 cases of them. The results showed that 11 cases (8.9%) were Ph negative; 113 cases (91.1%) were Ph positive; 104 cases (83.9%) had standard Ph translocation; 9 cases (7.2%) showed variant translocation, including 4 cases (3.2%) with simple variant translocation and 5 cases (4.0%) with complex variant translocation. 72.6% of Ph negative CML-BC had extra chromosomal abnormalities, with the most common ones being i (17q) and +14. 72.3% of Ph positive CML-BC had extra cytogenetic changes and +Ph, +8, i (17q) were the most frequent. The average time between diagnosis and blast crisis was 29.0 months (range 2 to 66 months) for Ph negative cases and 34.2 months (range 1 to 127 months) for Ph positive cases. 5 cases (5/28, 17.9%) with der (9) deletions were detected by FISH technique. It is concluded that extra chromosomal abnormalities are common in CML-BC patients and FISH can effectively detect the der (9) deletions.
- Published
- 2007
45. [Molecular cytogenetic characteristics of chronic lymphocytic leukemia]
- Author
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Wei, Xu, Jian-yong, Li, Jin-lan, Pan, Hai-rong, Qiu, Yun-feng, Shen, Bing, Xiao, Li-juan, Chen, Ya-fang, Wu, Rui-lan, Sheng, and Yong-quan, Xue
- Subjects
Aged, 80 and over ,Male ,Chromosomes, Human, Pair 12 ,Chromosomes, Human, Pair 13 ,Humans ,Female ,Trisomy ,Chromosome Deletion ,Middle Aged ,Leukemia, Lymphocytic, Chronic, B-Cell ,In Situ Hybridization, Fluorescence ,Aged ,Chromosomes, Human, Pair 17 - Abstract
To explore the molecular cytogenetic characteristics in patients with chronic lymphocytic leukemia (CLL).Interphase fluorescence in situ hybridization (FISH) was used to detect trisomy 12, deletion of 13q14 and 17p13 in 60 patients with CLL.Out of the 60 patients, 41 (68.3%) had at least one kind of molecular cytogenetic aberrations. Two (3.3%) had two kinds of abnormalities. Trisomy 12 was found in 12 (20.0%) cases, 13q14 deletion in 24 (40.0%) cases and 17p13 deletion in 5 (11.7%) cases. The number of trisomy 12 cells ranged from 4.0% to 34.0%, 13q14 deletion ranged from 22.0% to 93.0% and 17p13 deletion ranged from 6.0% to 68.0%. There was no significant difference among each Binet stages.FISH is a more rapid, accurate and sensitive technique in analysis of chromosome aberrations in CLL. FISH may provide accurate information of molecular cytogenetics for CLL.
- Published
- 2006
46. [The value of multiplex fluorescence in situ hybridization in the detection of complex karyotypic abnormalities of acute myeloid leukemia]
- Author
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Li, Ma, Jian-yong, Li, Jin-lan, Pan, Bing, Xiao, Si-xuan, Qian, Li-juan, Chen, Hai-rong, Qiu, Bing-zhao, Wen, and Yong-quan, Xue
- Subjects
Adult ,Male ,Young Adult ,Adolescent ,Leukemia, Myeloid ,Acute Disease ,Spectral Karyotyping ,Humans ,Female ,Middle Aged ,Translocation, Genetic - Abstract
To investigate the value of multiplex fluorescence in situ hybridization (FISH) in the detection of complex karyotypic abnormalities of acute myeloid leukemia (AML).Multiplex FISH was used in combination with conventional cytogenetics (CC) and interphase FISH to study 14 cases of AML with complex karyotypic abnormalities.In the 14 cases of AML studied, conventional cytogenetics detected 23 numerical and 56 structural chromosome abnormalities. Among them 4 gained whole chromosome and 4 lost whole chromosome which were confirmed by multiplex FISH. Twelve chromosome losses detected by CC were revised as derivative chromosomes resulted from various structural aberrations, and 26 derivative and 19 marker chromosomes were characterized precisely by multiplex FISH. Most of them were resulted from unbalanced translocations, including 2 complex 8; 21 translocations, which have not been reported previously: t (8; 21), der (8) t (8; 21) (8pter --8q22::21q22 --21qter), der (21) t (8; 21; 8) (8qter --8q22:: 21p13 --21q22::8q22 --8qter) and t (21; 8; 18; 1), der (8) t (8; 21) (8pter --8q22:: 21q22 --21qter), der (21) t (21; 8; 18; 1) (21p13 --21q22?::8q22 --8q24 ?:: 18??::1q??q??). The complex karyotypic abnormalities involved nearly all chromosomes, of which the chromosomes 17, 7 and 5 were more involved than the rest.Multiplex FISH in combination with conventional cytogenetics may characterize the complex chromosomal abnormalities more precisely. Introduction of this technique to the study of AML with complex chromosomal abnormalities is warranted.
- Published
- 2006
47. [Study of deletion of derivative chromosome 9 in patients with Ph+ chronic myeloid leukemia]
- Author
-
Wei, Wu, Yong-quan, Xue, Ya-fang, Wu, Jin-lan, Pan, and Juan, Shen
- Subjects
Adult ,Male ,Adolescent ,Middle Aged ,Prognosis ,Translocation, Genetic ,Survival Rate ,Young Adult ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Humans ,Female ,Philadelphia Chromosome ,Chromosome Deletion ,Child ,Chromosomes, Human, Pair 9 ,In Situ Hybridization, Fluorescence ,Aged - Abstract
To determine the frequency of the derivative 9 [der(9)] deletion among chronic myeloid leukemia (CML) patients with classic and variant Ph translocations, and assess the correlation between this deletion and clinical prognosis.Cytogenetic analysis of bone marrow cells was performed by direct method and/or 24 h culture method. RHG banding was used for karyotype analysis. Dual-color and dual-fusion DNA probe was used to perform FISH for investigating the deletion of der(9) in Ph+ CML patients.Cytogenetics studies showed typical Ph translocation in 76/105 and variant Ph translocation in 29/105 cases. Interphase-FISH studies showed deletion of der(9) in 12 (15.8%) of 76 patients with classic Ph translocation and in 4 (13.7%) of 29 patients with variant translocation. The frequency of deletion was similar in classic and variant translocations (P0.05). When the deletion was seen in the patient, it was present in all the Ph+ metaphases and nuclei. In 3 patients there were mixed cell populations with either 5'-abl or 3'-bcr deletion and all the 3 patients had both 5'-abl and 3'-bcr deletion. The median survival time of patients with deletion was significantly shorter than those without deletion (34 months vs 76 months; P0.05).Deletion of der(9) is seen in about 1/6 of Ph+ CML patients in our study on Chinese CML patients, Ph+ CML patients with the deletion have shorter median survival time than those without it, indicating that it is a poor prognostic index.
- Published
- 2006
48. [Detection of fusion genes associated with specific translocations in acute leukemia patients with normal karyotypes by using multiplex RT-PCR]
- Author
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Li, Ma, Yong-Quan, Xue, Jin-Lan, Pan, Jun, He, Ya-Fang, Wu, Jian-Nong, Cen, and Bin-Zhao, Wen
- Subjects
Adult ,Aged, 80 and over ,Male ,Leukemia ,Adolescent ,Oncogene Proteins, Fusion ,Reverse Transcriptase Polymerase Chain Reaction ,Middle Aged ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Prognosis ,Translocation, Genetic ,Leukemia, Myeloid, Acute ,Karyotyping ,Acute Disease ,Cytogenetic Analysis ,Humans ,Female ,Aged - Abstract
This study was aimed to explore the usefulness of multiplex reverse transcription-polymerase chain reaction (multiplex RT-PCR) in detection of fusion genes associated with specific translocations in acute leukemia (AL) patients with normal karyotypes. 37 AL patients with normal karyotypes were analyzed by multiplex RT-PCR. The results showed that 4 types of fusion genes such as PML/RARA, AML1/ETO, CBFbeta/MYH11, BCR/ABL were detected in 8 (21.6%) patients by multiplex RT-PCR. In conclusion, multiplex RT-PCR is useful in detection of fusion genes associated with specific translocations in acute leukemia (AL) with normal karyotypes and it would refine the karyotype analysis. When the normal karyotypes were detected in acute leukemia patients by conventional cytogenetic method, the multiplex RT-PCR should be performed for them.
- Published
- 2006
49. [Detection of the complex chromosomal aberrations in acute lymphoblastic leukemia by means of multiplex fluorescence in situ hybridization]
- Author
-
Jian-Yong, Li, Li, Ma, Bing, Xiao, Jin-Lan, Pan, Hai-Rong, Qiu, Ya-Fang, Wu, Bing-Zhao, Wen, and Yong-Quan, Xue
- Subjects
Adult ,Chromosome Aberrations ,Male ,Adolescent ,Karyotyping ,Cytogenetic Analysis ,Humans ,Female ,Middle Aged ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,In Situ Hybridization, Fluorescence - Abstract
This study was aimed to establish the technique of multiplex fluorescence in situ hybridization (M-FISH) and to explore its usefulness in detection of complex chromosomal aberrations (CCAs) in acute lymphoblastic leukemia (ALL). Five ALL patients with CCAs were analyzed by combining the techniques of conventional cytogenetics (CC) and M-FISH. The results demonstrated that M-FISH confirmed the aberrations previously detected by CC, such as t (9;22), t (1;19) and t (y;1), and revealed new abnormalities as der (1) (1::3::7), der (6) t (6;9) (q?;p13), der (1) t (1;11), der (12) t (1;12), der (3) t (3;5), der (2) t (2;16), der (9) (9::18::7) and der (7) (9::18::7), and also corrected the wrong results in CC. Among these abnormalities, der (9) (9::18::7) and der (7) (9::18::7) were reported for the first time. In conclusion, M-FISH has proved to be useful in characterization of the CCAs in ALL, and it is an essential method to refine the karyotype analysis.
- Published
- 2006
50. [Multiplex fluorescence in situ hybridization in detecting complex chromosomal aberrations in myelodysplastic syndromes]
- Author
-
Bing, Xiao, Jian-yong, Li, Jin-lan, Pan, Li, Ma, Hai-rong, Qiu, Ya-fang, Wu, and Yong-quan, Xue
- Subjects
Adult ,Chromosome Aberrations ,Gene Rearrangement ,Male ,Adolescent ,Karyotyping ,Myelodysplastic Syndromes ,Humans ,Female ,Middle Aged ,In Situ Hybridization, Fluorescence ,Translocation, Genetic ,Aged - Abstract
To explore the value of multiplex fluorescence in situ hybridization (M-FISH) technique in the detection of the complex chromosomal aberrations (CCAs) in myelodysplastic syndromes (MDS).M-FISH was used in ten MDS patients with R-banding CCAs to refine the complex chromosomal rearrangements, the constitute of marker chromosomes, and to identify the cryptic translocations.Thirty-seven kinds of structural rearrangements were detected by M-FISH including insertion, deletion, translocation and derivative chromosomes, among which 34 kinds were unbalanced rearrangements, and 3 were balanced rearrangements including t(6;22) (q21; q12), t(9; 19) (q13; p13) and t(3;5) (?; ?). Seven abnormalities in the present paper were first reported in the literature. In addition, chromosome 17 aberrations (7/10) and -5/5q - (7/10) were the two most frequent abnormalities.M-FISH could refine CCAs in MDS patients, find or correct the missed or misidentified abnormalities analysed by conventional cytogenetics.
- Published
- 2006
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