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211 results on '"Jin Fengliang"'

1. Control efficiency and mechanism of spinetoram seed-pelleting against the striped flea beetle Phyllotreta striolata

Author

Xiong Tengfei, Satyabrata Nanda, Jin Fengliang, Lin Qingsheng, and Feng Xia

Subjects
Medicine, Science
Abstract

Abstract The striped flea beetle (SFB, Phyllotreta striolata) can cause serious harm to cruciferous crops in both the larval and adult stages. Presently, there are no other sustainable alternatives to the use of chemical pesticides for controlling SFB infestation. In this study, the use of a seed-pelletized coating of spinetoram effectively reduced the numbers of SFB and its feedings on the flowering cabbage seedlings, whereas, in combination with the insect-proof net, it controlled the SFB infestation throughout the cabbage growth period. The analysis of the pesticide residues in soil and different cabbage parts indicated the degradation dynamics of spinetoram. The concentration of spinetoram in cabbage parts decreased over time, while increased first and subsequently decreased in soil. Furthermore, estimation of the half-life of spinetoram revealed that via seed-palletized application spinetoram half-life was found to be 2.82 days in soil, 4.21 days in the root, 5.77 days in the stem, and 3.57 days in the leaf, respectively. Both the lower pesticide residues and the half-life of spinetoram in soil and cabbage parts suggested it to be a promising environment and food-safe pesticide in controlling SFB. Moreover, the seed-pelletized coating ensured a sustainable release of spinetoram that can reduce the pesticide application frequency and be cost-effective and pocket-friendly for the farmers.

Published
2022
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6. Metagenomics and Microbial Ecology

Author

De Mandal, Surajit, primary, Panda, Amrita Kumari, additional, Kumar, Nachimuthu Senthil, additional, Bisht, Satpal Singh, additional, and Jin, Fengliang, additional

Published
2021
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7. Two-Sex Life Table Analysis for Optimizing Beauveria bassiana Application against Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae).

Author

Zafar, Junaid, Shoukat, Rana Fartab, Zhu, Zhanpeng, Fu, Dongran, Xu, Xiaoxia, and Jin, Fengliang

Subjects
BEET armyworm, BEAUVERIA bassiana, INSECT pests, LIFE tables, NOCTUIDAE, METABOLIC detoxification, BIOPESTICIDES
Abstract

Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) is a highly dispersive, polyphagous insect pest that severely defoliates crops. Excessive reliance on synthetic insecticides leads to ecological pollution and resistance development, urging scientists to probe eco-friendly biopesticides. Here, we explore the virulence of an entomopathogenic fungus, Beauveria bassiana, against S. exigua, resulting in 88% larval mortality. Using an age–stage, two-sex life table, we evaluated the lethal and sublethal effects of B. bassiana on the demographic parameters of S. exigua, including survival, development, and reproduction. Sublethal (LC20) and lethal concentrations (LC50) of B. bassiana impacted the parental generation (F0), with these effects further influencing the demographic parameters of the first filial generation (F1). The infected F1 offsprings showed a reduced intrinsic rate of increase (r), mean generation time (T), and net reproduction rate (R0). Larval developmental duration varied significantly between the control (10.98 d) and treated groups (LC20: 10.42; LC50: 9.37 d). Adults in the treated groups had significantly reduced lifespans (M: 8.22; F: 7.32 d) than the control (M: 10.00; F: 8.22 d). Reduced fecundity was observed in the B. bassiana-infected groups (LC20: 313.45; LC50: 223.92 eggs/female) compared to the control (359.55 eggs/female). A biochemical assay revealed elevated levels of detoxification enzymes (esterases, glutathione S-transferases, and acetylcholinesterase) in the F0 generation after B. bassiana infection. However, the enzymatic activity remained non-significant in the F1 generation likely due to the lack of direct fungal exposure. Our findings highlight the enduring effects of B. bassiana on the biological parameters and population dynamics of S. exigua, stressing its use in eco-friendly management programs. [ABSTRACT FROM AUTHOR]

Published
2024
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17. MiR-2b-3p Downregulated PxTrypsin-9Expression in the Larval Midgut to Decrease Cry1Ac Susceptibility of the Diamondback Moth, Plutella xylostella(L.)

Author

Zhang, Jie, Liu, Mingyou, Wen, Liang, Hua, Yanyan, Zhang, Ruonan, Li, ShuZhong, Zafar, Junaid, Pang, Rui, Xu, Hanhong, Xu, Xiaoxia, and Jin, Fengliang

Abstract

Crystal (Cry) toxins, produced by Bacillus thuringiensis, are widely used as effective biological pesticides in agricultural production. However, insects always quickly evolve adaptations against Cry toxins within a few generations. In this study, we focused on the Cry1Ac protoxin activated by protease. Our results identified PxTrypsin-9as a trypsin gene that plays a key role in Cry1Ac virulence in Plutella xylostellalarvae. In addition, P. xylostellamiR-2b-3p, a member of the micoRNA-2 (miR-2) family, was significantly upregulated by Cry1Ac protoxin and targeted to PxTrypsin-9downregulated its expression. The mRNA level of PxTrypsin-9, regulated by miR-2b-3p, revealed an increased tolerance of P. xylostellalarvae to Cry1Ac at the post-transcriptional level. Considering that miR-2b and trypsin genes are widely distributed in various pest species, our study provides the basis for further investigation of the roles of miRNAs in the regulation of the resistance to Cry1Ac and other insecticides.

Published
2024
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29. BmMD‐2A responds to 20‐hydroxyecdysone and regulates Bombyx mori silkworm innate immunity in larva‐to‐pupa metamorphosis.

Author

Zhang, Ruonan, Chen, Xue, Wang, Yuan, Bai, Xu, Yang, Qiong, Zhong, Yangjin, Yu, Xiao‐Qiang, Jin, Fengliang, and Yang, Wanying

Subjects
SILKWORMS, NATURAL immunity, INSECT metamorphosis, ANTIMICROBIAL peptides, CELLULAR signal transduction, METAMORPHOSIS, STAPHYLOCOCCUS aureus, STAPHYLOCOCCUS, MOLTING
Abstract

20E‐hydroxyecdysone (20E) plays important roles in larval molting and metamorphosis in insects and is also involved in the insect innate immune response. Insect metamorphosis is a highly successful strategy for environmental adaptation and is the most vulnerable stage during which the insect is susceptible to various pathogens. 20E regulates a series of antimicrobial peptides (AMPs) through the immunodeficiency (IMD) pathway activation in Drosophila; nevertheless, whether other immune pathways are involved in 20E‐regulated insect immunity is unknown. Our previous studies showed that BmMD‐2A is a member of the MD‐2‐related lipid recognition (ML) family of proteins that are involved in the Bombyx mori innate immunity Toll signaling pathway. In this study, we further demonstrate that BmMD‐2A is also positively regulated by 20E, and the BmMD‐2A neutralization experiment suggested that 20E activates some downstream immune effect factors, the AMP genes against Escherichia coli and Staphylococcus aureus, through the regulation of BmMD‐2A in larval metamorphosis, implying that B. mori may use the Toll‐ML signaling pathway to maintain innate immune balance in the larval‐pupal metamorphosis stage, which is a different innate immunity pathway regulated by 20E compared to the IMD pathway in Drosophila. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Control Efficiency and Mechanism of Spinetoram Seed-Pelleting Against the Striped Flea Beetle Phyllotreta Striolata

Author

Xiong, Tengfei, Satyabrata, Nanda, Jin, Fengliang, Lin, Qingsheng, and Feng, Xia

Subjects
Coleoptera, Insecticides, Soil, Multidisciplinary, Seeds, fungi, Pesticide Residues, Animals, Siphonaptera, food and beverages, Brassica, Macrolides
Abstract

The striped flea beetle (SFB, Phyllotreta striolata) is an important pest of the cruciferous crops in Asia. SFB is regarded as the most destructive pest of cruciferous crops in China due to the severe crop loss and frequent infestation incidents. As no SFB resistant cultivar is available at present, therefore, application of insecticides is the primary method of SFB control. On the contrary, the exploitation of chemical insecticides causes severe environmental issues and is not cost-effective. The use of a seed-pelletized coating of spinetoram effectively reduced SFB feedings on the flowering cabbage seedlings, whereas in combination with the insect-proof net, it controlled the SFB infestation throughout the cabbage growth period. The analysis of the pesticide residues in soil and different cabbage parts indicated the degradation dynamics of spinetoram. Furthermore, estimation of the half-life of spinetoram revealed that via seed-palletized application spinetoram half-life was found to be 2.82 days in soil, 4.21 days in the root, 5.77 days in the stem, and 3.57 days in the leaf, respectively. Both the lower pesticide residues and the half-life of spinetoram in soil and cabbage parts suggested it to be a promising environment and food-safe pesticide in controlling SFB. Moreover, the seed-pelletized coating ensured a sustainable release of spinetoram that can reduce the pesticide application frequency and be cost-effective and pocket-friendly for the farmers.

Published
2021

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