60 results on '"Jin-Hai Zhang"'
Search Results
2. Rapid screening of Zr-containing particles from Chang’e-5 lunar soil samples for isotope geochronology: Technical roadmap for future study
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Jin-Hua Li, Qiu-Li Li, Liang Zhao, Jin-Hai Zhang, Xu Tang, Li-Xin Gu, Qian Guo, Hong-Xia Ma, Qin Zhou, Yan Liu, Pei-Yu Liu, Hao Qiu, Gang Li, Lin Gu, Shun Guo, Chun-Lai Li, Xian-Hua Li, Fu-Yuan Wu, and Yong-Xin Pan
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QE1-996.5 ,Extraterrestrial samples ,µXRF ,Correlative workflow ,Technical roadmap ,Chang’e-5 lunar samples ,General Earth and Planetary Sciences ,Geology ,Zr-containing particles - Abstract
New samples returned by China Chang’e-5 (CE-5) mission offer an opportunity for studying the lunar geologic longevity, space weathering, and regolith evolution. The age determination of the CE-5 samples was among the first scientific questions to be answered. However, the precious samples, most in the micrometer size range, challenge many traditional analyses on large single crystals of zircon developed for massive bulk samples. Here, we developed a non-destructive rapid screening of individual zirconium-containing particle for isotope geochronology based on a Micro X-ray fluorescence analysis (µXRF). The selected particles were verified via scanning electron microscopy (SEM), 3D X-ray microscopy (XRM), and focused ion beam scanning electron microscopy (FIB-SEM) techniques, which showed that zirconium-bearing minerals with several microns were precisely positioned and readily suitable for site-specific isotopic dating by second ion mass spectrometry (SIMS). Such protocol could be also applicable in non-destructively screening other types of particles for different scientific purposes. We therefore proposed a correlative workflow for comprehensively studying the CE-5 lunar samples from single particles on nanometer to atomic scales. Linking various microscopic and spectromicroscopic instruments together, this workflow consists of six steps: (1) single-particle selection with non-destructive µXRF technique, (2) 2D/3D morphological and structural characterization with a correlative submicron 3D XRM and nanoscale resolution FIB-SEM imaging methods, (3) SEM analysis of the surface morphology and chemistry of the selected particle, (4) a series of microscopic and microbeam analyses (e.g., SEM, electron probe microanalysis, and SIMS) on the cross-section of the selected particle to obtain structural, mineralogical, chemical, and isotopic features from the micron to nanometer scale, (5) advanced 2D/3D characterization and site-specific sample preparation of thin foil/tip specimens on a microregion of interest in the selected particle with FIB-SEM technique, and (6) comprehensive analyses on the FIB-milled specimens at nanometer to atomic scale with synchrotron-based scanning transmission X-ray microscopy, analytic transmission electron microscopy, and atom probe tomography. Following this technical roadmap, one can integrate multiple modalities into a uniform frame of multimodal and multiscale correlated datasets to acquire high-throughput information on the limited or precious terrestrial and extraterrestrial samples.
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- 2022
3. Research of Network Information Platform Construction of ERP System in Manufacturing
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Jin Hai Zhang
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Information management ,Engineering management ,Engineering ,Knowledge management ,business.industry ,Robustness (computer science) ,Competitive pressure ,General Medicine ,Informatization ,business - Abstract
Computer network system is a basis for enterprises to implement informatization construction , to a good network environment, System suitability, and security features of the system. Facing growing international and domestic competitive pressure, companies propose the development of a comprehensive information management strategy; in the process of enterprise Informationization construction, very important thing about the construction of network study environment for enterprises. Papers in reconciling efficiency, robustness, and security of the system on the basis of focusing on system "against" and "applicable" were studied.
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- 2014
4. Design and Implementation of Data Mining Based on Distributed Computing
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Jin Hai Zhang
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Database ,Data stream mining ,Computer science ,business.industry ,Distributed computing ,Computer data storage ,InformationSystems_DATABASEMANAGEMENT ,The Internet ,General Medicine ,Data mining ,computer.software_genre ,business ,computer - Abstract
Beacause internet data has a massive, diverse, heterogeneous, dynamic features, using traditional databases to analyze these data, data storage and processing efficiencies already can not meet the requirements. Utilizing leading-edge distributed computing technology to solve traditional data mining scenarios in lack of data mining of massive data improved data mining algorithm of lot OK Hadoop distributed computing platform, which later on other data mining algorithms using Hadoop to reference while using rich data mining algorithms can be found there is more value in your data.
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- 2014
5. Design of Ship Communication System Based on CAN Bus
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Jin Hai Zhang and Xi Kun Wang
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Flexibility (engineering) ,Engineering ,business.industry ,Reliability (computer networking) ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,General Medicine ,Communications system ,Automotive electronics ,CAN bus ,Embedded system ,business ,Fieldbus ,Protocol (object-oriented programming) ,System bus - Abstract
Existing ship electronic equipment are based on serial interfaces in accordance with NEMA0183 Protocol for the exchange of information, complex low transfer rates, lines, nodes exist, poor reliability shortcomings. Fieldbus (CAN) to its high anti-interference ability and higher traffic rates made up for these shortcomings, has been widely applied to automotive electronics, but in application of ship data communication has not been very good. CAN bus technology with outstanding reliability, timeliness and flexibility for the development of modern marine communication system offers a new way.
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- 2014
6. Research and Design of the Communication Middleware for the Distributed System
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Jin Hai Zhang
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Unix ,Distributed Computing Environment ,Location transparency ,Computer science ,Distributed computing ,General Medicine ,Transparency (human–computer interaction) ,computer.software_genre ,Inter-process communication ,Middleware ,Middleware (distributed applications) ,Daemon ,User interface ,computer ,Message queue - Abstract
This article on inter-process communication mechanism has conducted in-depth research and analysis of traditional UNIX system under the environment of the inter process communication mechanism and existing mechanisms for process communication in distributed environment. And on that basis propose new interprocess communication mechanism in distributed environment. Given interprocess communication semaphores and Message Queuing design ideas and implementation details, and compare it with the traditional way, we concluded that in the case of performance does not deteriorate, provides better user interfaces and location transparency and access higher transparency.
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- 2014
7. Study on Embedded TCP_IP Protocol and ARM Implementation
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Jin Hai Zhang
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Engineering ,TCP acceleration ,business.industry ,computer.internet_protocol ,Transmission Control Protocol ,Datagram ,Serial Line Internet Protocol ,General Medicine ,IP tunnel ,Internet protocol suite ,Embedded system ,Zeta-TCP ,business ,Communications protocol ,computer ,Computer network - Abstract
Rapid development of Internet networks and backbones technology makes communication based on packet-switching technology, quality and performance have been steadily improved. Existing embedded Internet scenarios, there are high costs, insufficient to achieve complex control functions such as. Embedded devices in the application of limited hardware resources, depending on application requirements simplified TCP/IP protocol. Research of embedded system based on TCP/IP to embedded information product development technology will undoubtedly have an important value.
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- 2014
8. Research on Navigation Class Specialized Student of Honor in China
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Xin Gang Song, Jin Hai Zhang, Jing Tao Ren, Jin Ming Hao, and Zhao Xuan Li
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Class (computer programming) ,Engineering ,Operations research ,business.industry ,Time constrained ,Crew ,General Medicine ,Price system ,Public relations ,Social relation ,Politics ,Honor ,China ,business - Abstract
Concept of honor is based on certain historical conditions and social relations, while guiding people to foster a correct world Outlook and Outlook on life and at the same time constrained by a world Outlook and Outlook on life. The shipping industry is an important window for the international society of China, crew's mental Outlook reflects the image of the Chinese nation. In maritime activities, the crew can properly establish the concept of honor, not only affecting their own scientific Outlook on life and values, indicating, Also determines the speed of shipping industry development in China and the success or failure of international political, economic and cultural exchanges.
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- 2014
9. Study on the Phenomenon of College Students' Network had a Crush on and the Educational Guidance
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Song Song Zhao, Zhen Xun Cui, Jin Hai Zhang, and Hua Jing Zhang
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Mental development ,Engineering ,business.industry ,media_common.quotation_subject ,Addiction ,Subject (philosophy) ,General Medicine ,Popularity ,Political education ,Phenomenon ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Quality (business) ,Ideology ,business ,media_common - Abstract
With the popularity and development of application of network in colleges and universities, students enjoy the convenience of learning and school life network at the same time, it could also be that excessive and irregular use and create network obsession or addiction led directly to an increase in psychological problems among college students, lack of ideals and beliefs, moral quality of landslide, thus affecting the students ' healthy and smooth growth. How to educating and guiding college students, the prevention and resolution of network with negative consequences for physical and mental development of college students, into a new era, the new situation of ideological and political education of college students under the subject of intense interest.
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- 2014
10. Design of Real-Time Streaming Media Distribution MStreaming System
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Hua Jing Zhang, Jin Hai Zhang, and Song Song Zhao
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Media management ,Computer science ,business.industry ,computer.internet_protocol ,Quality of service ,Distributed computing ,Usability ,General Medicine ,computer.software_genre ,Bottleneck ,Systems management ,Real Time Streaming Protocol ,business ,computer ,Computer network - Abstract
This article taking into account the network efficiency, QoS, resource consumption, latency, performance and ease of use, and implementation of the system on the basis of factors such as the complexity, media management, node in the system management, and streaming media QoS control, buffer management, incentives, and other key algorithm and protocol analysis and design, and trying to achieve a balance between the factors. Finally developed real-time streaming data distribution system based on P2P MStreaming program, its resources quickly and easily launch and positioning, exert the advantage of P2P technology can effectively solve the problem of server bottleneck link, decentralized services and resource consumption.
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- 2014
11. Research on Inquiry Teaching of Computer Programming
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Xin Gang Song, Lin Sun, Jin Hai Zhang, and Lin Jie Fan
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Subjectivity ,business.industry ,Computer science ,Scientific development ,Teaching method ,Teaching and learning center ,Computer programming ,Lifelong learning ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,General Medicine ,business ,Curriculum - Abstract
Provide opportunities for educational and scientific development, but also poses a challenge. Era of traditional teaching methods cannot meet the manpower needs of new curriculum reform requires renewing teaching ideas, changing teaching methods, reflect students ' subjectivity in the study and cultivation of students ' innovative spirit, formed the awareness and capability for lifelong learning. Inquiry teaching to cultivate students ' innovative spirit and practical ability for objective, reflect and respond to the demand for education in the era, are effective means of implementation of the new curriculum.
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- 2014
12. Research of Distributed P2P Network Streaming Media Technology
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Jin Hai Zhang
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Service (systems architecture) ,Multimedia ,business.industry ,Computer science ,computer.internet_protocol ,General Medicine ,computer.software_genre ,Live streaming ,Personal computer ,Bandwidth (computing) ,The Internet ,Real Time Streaming Protocol ,business ,computer ,Computer network - Abstract
With the popularity of the Internet, personal computer performance and network bandwidth continues to increase and the rapidly improving network streaming media applications have become more abundant. Due to the high bandwidth for streaming media have characteristics, traditional methods of C/S can easily cause server performance bottlenecks, introduce the idea of P2P live streaming media service, through normal computer exercise part of the server's functionality, decentralization of services, can effectively reduce the load on the server, as well as local high network bandwidth consumption. Therefore, P2P live streaming media distribution technology is potentially broad application prospects.
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- 2014
13. Research and Design of Image Acquisition System Using USB2.0 Interface
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Jin Hai Zhang
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Computer science ,business.industry ,Interface (computing) ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Image processing ,General Medicine ,Analog image processing ,Camera interface ,Digital image ,Automatic image annotation ,Transmission (telecommunications) ,Digital image processing ,Computer vision ,Artificial intelligence ,business ,Computer hardware - Abstract
Image acquisition is to study an image acquisition, transmission and storage of information, is the basis of image processing technologies. Currently most of the image acquisition device through the computer interface in the image data into the computer, with high speed and large storage capacity of the computer complete the image acquisition. Traditional computer interface in image processing of data if there is a problem such as high cost, inconvenience, in response to this situation, this paper proposes a USB2.0 interface technology of image acquisition system programme.
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- 2013
14. Research on Software Product Development Project Management
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Jin Hai Zhang
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Engineering ,Knowledge management ,business.industry ,Software development ,General Medicine ,Application lifecycle management ,Software development process ,Engineering management ,Project planning ,Package development process ,Extreme project management ,business ,Software project management ,Project management triangle - Abstract
Project organization and management software development project management model is the best form of software development enterprises in China's understanding of software development project management has been greatly improved. Traditional software development projects have different characteristics, therefore how flexible application for software project management theory and strategies of the main research objectives of domestic software development project management. Our software in enterprise application software development project management, general shortcomings in implementation, this article on software research and development analysis and project management of implementation strategies.
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- 2013
15. Study of Embedded Operating System Process Scheduling
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Jin Hai Zhang
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Fixed-priority pre-emptive scheduling ,Software ,Computer science ,business.industry ,Embedded system ,Two-level scheduling ,General Medicine ,Dynamic priority scheduling ,business ,Round-robin scheduling ,Embedded operating system ,Fair-share scheduling ,Scheduling (computing) - Abstract
Embedded systems are widely used in industries, its amazing to develop speed and as an embedded system software for embedded operating system is at the core of the information industry research hot spots. This thesis is mainly embedded in different application environments, the operating system discussion and research on how to implement its process scheduling algorithm.
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- 2013
16. Dendritic development of hippocampal CA1 pyramidal cells in a neonatal hypoxia-ischemia injury model
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Shan Ou, Yan Dong Zhao, Sai Yu Cheng, Jin Hai Zhang, Zhi Xiao, Wen Juan He, and Huai Zhen Ruan
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Dendritic spine ,business.industry ,Ischemia ,food and beverages ,Morris water navigation task ,Hippocampal formation ,Hypoxia (medical) ,medicine.disease ,Neonatal hypoxia ,Muscle hypertrophy ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,nervous system ,chemistry ,Corticosterone ,Medicine ,medicine.symptom ,business ,Neuroscience - Abstract
It is believed that neonatal hypoxia–ischemia (HI) brain injury causes neuron loss and brain functional defects. However, the effect of HI brain injury on dendritic development of the remaining pyramidal cells of the hippocampus and the reaction of contralateral hippocampal neurons require further studies. The Morris water maze and Golgi–Cox staining were used to evaluate the learning and memory and dendritic morphology of pyramidal cells. The results of Golgi–Cox staining showed CA1 pyramidal neurons of HI injury models with fewer bifurcations and shorter dendrite length than the naive control group. The density of dendritic spines of hippocampal CA1 pyramidal neurons was significantly lower in the HI brain injury group than in controls. With respect to hippocampal function, the HI brain injury group presented cognitive deficits in the reference memory task and probe trail. In the HI group, the pyramidal cells of left hippocampus that did not experienced ischemia but did experience hypoxia had more complex dendrites and higher density of spine than the HI injury side and control. The functional implementation of injured hippocampus might depend mainly on the hypertrophy of contralateral hippocampus after HI brain injury. Corticosterone can partially prevent the hippocampal pyramidal cells from HI injury and reduce the difference of the bilateral hippocampus pyramidal cells, but there was no improvement in learning and memory. © 2013 Wiley Periodicals, Inc.
- Published
- 2013
17. Study on Classification of Network Streaming Media Encryption
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Jin Hai Zhang
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Digital image ,Multimedia ,business.industry ,Computer science ,computer.internet_protocol ,Process (computing) ,The Internet ,Real Time Streaming Protocol ,General Medicine ,business ,Encryption ,computer.software_genre ,computer - Abstract
Rapid development of the Internet, to provide streaming media business emergence of soil to grow, with the updated development of streaming media technology, it security problems more and more attention. In view of the breadth of media stream data storage and transfer, and easy features, making it more vulnerable to damage and attack, and the entire process has had to face very large data flows. Therefore, encryption of digital images and video special research is necessary on the issue.
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- 2013
18. Design and Realization of RFID Cold Chain Logistics Management System
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Bo Ye and Jin Hai Zhang
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Engineering ,Software ,business.industry ,Supply chain ,Logistics management ,General Medicine ,Cold chain ,business ,Automation ,Realization (systems) ,Manufacturing engineering - Abstract
RFID technology as one of the top ten technology of the 20th century, has been in transportation, logistics, industrial and other fields has been more and more widely applied, and are increasingly showing its great potential. RFID cold chain logistics management system to promote the implementation of automation, intelligence, and integration of software applications such as supply chain will play a great role in promoting.
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- 2013
19. Research on the Embedded Network Streaming Media Technology
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Jin Hai Zhang
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Digital rights management ,Multimedia ,computer.internet_protocol ,Computer science ,Real Time Streaming Protocol ,General Medicine ,Space (commercial competition) ,computer.software_genre ,computer - Abstract
With the development of the information industry, awareness of information resources has gradually transition from text and images to audio and video, and an increased emphasis on getting real-time and interactive resources. Streaming media with start delay is small, does not take the client's local storage space advantages, gradually became the preferred. Streaming media applications based on IP networks continuous development on a global scale.
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- 2013
20. Research and Design of Experiment Platform Based on ARM Embedded System
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Jin Hai Zhang
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Engineering ,Automatic Identification System ,business.industry ,Design of experiments ,Information technology ,General Medicine ,GeneralLiterature_MISCELLANEOUS ,law.invention ,Maritime security ,law ,Information and Communications Technology ,Embedded system ,Wi-Fi ,Architecture ,Radar ,business - Abstract
With the development of the global economy, national exchange frequently between navigational skills improve more and more attention. Radar, ARPA has identified the ship's capabilities, but because of their limitations were unable to adapt to the needs of modern maritime security. As digital communications technology, the rapid development of computer and network information technology, development of AIS Technology Foundation has been set up. Navigational aids in the shipping industry have become increasingly demanding of circumstances, marine automatic identification system (AIS) came into being. This article examines the AIS system architecture based on wireless local area network, building a wireless local area network.
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- 2013
21. Research on Mathematical Modeling of Ship Simulation System
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Jin Hai Zhang and Yong Xiang Li
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Engineering ,Mathematical model ,Operations research ,business.industry ,Teaching method ,Control (management) ,General Medicine ,Simulation system ,Work (electrical) ,Systems engineering ,Driving simulation ,Process control ,Production (economics) ,business - Abstract
With the rapid development of science and technology, mathematical models more and more present in modern production, work and social activities. Must be established to control the production process of mathematical models, use this model on design and calculation of control devices accordingly in order to achieve effective process control. Ship handling Simulator as an advanced teaching method applied in the teaching and training of crews sailing in China have a long history. By driving simulation of ship navigation, training students to train and ship very similar environment.
- Published
- 2013
22. [Clinical study of application minimally invasive expandable channel in lumbar discectomy and interbody fusion and internal fixation]
- Author
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Yong-Qing, He, Chang, Xiang, Jin-Hai, Zhang, Chao-Yang, Ruan, Gang, Zhang, Qiang-Hua, Zeng, Qun-Wei, Zhu, Sheng-Wen, Li, and Zheng, Qian
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Adult ,Male ,Lumbar Vertebrae ,Spinal Fusion ,Treatment Outcome ,Humans ,Minimally Invasive Surgical Procedures ,Female ,Middle Aged ,Aged ,Diskectomy ,Retrospective Studies - Abstract
To explore the advantages of minimally invasive expandable in surgery of lumbar discectomy and interbody fusion and internal fixation.The clinical data of 48 patients who underwent lumbar discectomy and interbody fusion and internal fixation from January 2010 to March 2016 was retrospectively analyzed. According to the admission queue, the patients were randomly assigned into channel group (26 cases) or traditional group (22 cases). In channel group, surgical approach of minimally invasive expandable channel was applied, and in traditional group, open posterior operation approach (including posterior lumbar interbody fusion and transforaminal lumbar interbody fusion, etc.) was applied. In channel group, there were 20 males and 6 females, aged from 43 to 74 years with an average of(56.6±5.1) years; course of disease was ranged from 4 to 22 months with an average of (6.7±1.8) months; 1 case was complicated with diabetes, 6 cases were complicated with hypertensive disease, and 2 cases were complicated with arrhythmia. In traditional group, there were 15 males and 7 females, aged from 43 to 73 years with an average of(55.9±4.6) years; course of disease was ranged from 4 to 26 months with an average of (6.2±2.1) months; 2 cases were complicated with diabetes, 5 cases were complicated with hypertensive disease, and 1 case was complicated with arrhythmia. Operation time, bleeding volume, and hospitalization time were compared between two groups and visual analogue scale(VAS), Oswestry Disability Index(ODI), bone fusion information, and complications correlated with incision were observed in two groups.All 48 patients were followed up for more than 6 months. Postoperative VAS and ODI were significantly improved (Compared with conventional surgery minimally invasive lumbar discectomy and interbody fusion and internal fixation has advantages of less trauma, shorter operative time and better functional recovery.
- Published
- 2016
23. Research on Oil Separator Control and Simulation
- Author
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Xiao Li and Jin Hai Zhang
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Engineering ,Engine room ,ComputingMethodologies_SIMULATIONANDMODELING ,business.industry ,General Engineering ,Separator (oil production) ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Electronic information ,business ,Process engineering ,Simulation ,Computer technology - Abstract
With the rapid development of computer technology and electronic information technology is widely used, and application of computer simulation technology is increasingly popular. Engine room Simulator is a typical application of computer simulation technology in the field of maritime, has become an important means of training, assessment of the majority of the crew. Among them, marine oil separator system is an important part of marine Simulator. Simulation program for marine oil separator system timing control simulation for oil separator, and simulation for common failures.
- Published
- 2012
24. Research of Automatic Fingerprint Recognition Essential Algorithms
- Author
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Jin Hai Zhang
- Subjects
Minutiae ,Computer science ,Fingerprint ,business.industry ,Data_MISCELLANEOUS ,Pattern recognition ,Theoretical research ,Computer vision ,General Medicine ,Artificial intelligence ,Fingerprint recognition ,business ,Algorithm - Abstract
Fingerprint recognition has wide application prospect in all fields which contain identity authentication. Construction of accurate and reliable,safe and Practical automatic fingerprint identification system(AFIS) has become researc hotspot. Although theoretical research and application developmen of AFIS have got a significant Progress,accuracy of the algorithm and proeessing speeds till need to be improved. In this paper, fingerprint image preprocessing algorithms,fingerprint singular Points and minutiae extraction algorithm and fingerprint matching algorithm are analyzed and discussed in detail.
- Published
- 2011
25. MANDELBROT AND JULIA SETS OF ONE-PARAMETER RATIONAL FUNCTION FAMILIES ASSOCIATED WITH NEWTON'S METHOD
- Author
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Zhi-Jie Li, Jin-Hai Zhang, Xiang-Dong Liu, and Xue-Ye Ang
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Discrete mathematics ,Applied Mathematics ,Mandelbrot set ,Julia set ,Misiurewicz point ,symbols.namesake ,Newton fractal ,Modeling and Simulation ,External ray ,symbols ,Geometry and Topology ,Complex quadratic polynomial ,Mandelbox ,Mathematics ,Pickover stalk - Abstract
In this paper, general Mandelbrot and Julia sets of one-parameter rational function families associated with Newton's method were discussed. The bounds of these general Mandelbrot sets and two formulas for calculating the number of different periods periodic points of these rational functions were given. The relations between general Mandelbrot sets and common Mandelbrot sets of zn + c (n ∈ Z, n ≥ 2), along with the relations between general Mandelbrot sets and their corresponding Julia sets were investigated. Consequently, the results were found in the study: there are similarities between the Mandelbrot and Julia sets of one-parameter rational function families associated with Newton's method and the Mandelbrot and Julia sets of zn + c (n ∈ Z, n ≥ 2).
- Published
- 2010
26. GENERALIZED SECANT METHODS AND THEIR FRACTAL PATTERNS
- Author
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Jun-Xing Zhang, Zhi-Jie Li, Jin-Hai Zhang, and Xiang-Dong Liu
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Hyperbolic secant distribution ,Applied Mathematics ,Mathematical analysis ,Chaotic ,Fractal landscape ,symbols.namesake ,Secant method ,Modeling and Simulation ,Fractal derivative ,Muller's method ,symbols ,Geometry and Topology ,Complex plane ,Newton's method ,Mathematics - Abstract
In this paper, a class of generalized secant methods was investigated. The convergence of these methods was discussed, and their striking fractal patterns are generated to represent the chaotic behavior of these methods in the complex plane.
- Published
- 2009
27. Inhibition of ATP-Induced Glutamate Release by MRS2179 in Cultured Dorsal Spinal Cord Astrocytes
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Huai-Zhen Ruan, Wen-Juan He, Xiao-Hong Liu, Jin-Hai Zhang, Xi-Gui Wu, Jun-Wei Zeng, and Lu Du
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Time Factors ,Glutamic Acid ,Rats, Sprague-Dawley ,Receptors, Purinergic P2Y1 ,Adenosine Triphosphate ,Mediator ,Purinergic P2 Receptor Antagonists ,medicine ,Animals ,heterocyclic compounds ,Receptor ,Cells, Cultured ,Chromatography, High Pressure Liquid ,Pharmacology ,Calcium metabolism ,Microscopy, Confocal ,Dose-Response Relationship, Drug ,Receptors, Purinergic P2 ,Chemistry ,musculoskeletal, neural, and ocular physiology ,Purinergic receptor ,Glutamate receptor ,General Medicine ,Spinal cord ,Rats ,Cell biology ,Adenosine Diphosphate ,Posterior Horn Cells ,medicine.anatomical_structure ,Astrocytes ,Excitatory postsynaptic potential ,Calcium ,Neuroscience ,Astrocyte - Abstract
It was reported that ATP, an excitatory chemical mediator, exerts its effects by activation of the P2X (ligand-gated cationic channels) and P2Y (G protein-coupled receptors) purinoceptors in the nervous system. In the present work, we used confocal laser scanning microscopy and high-performance liquid chromatography to assess the role of the P2Y1 receptor in ATP-evoked Ca2+ mobilization and glutamate release from cultured dorsal spinal cord astrocytes. ATP (0.01–100 µmol/l) produces a dose-dependent rise in the Ca2+ relative fluorescence intensity in cultured astrocytes. N6methyl-2′-deoxyadenosine-3′,5′-bisphosphate (MRS2179, 0.01–100 µmol/l), a P2Y1-specific antagonist, could dose-dependently inhibit ATP-evoked Ca2+ mobilization. In addition, 100 µmol/l ATP caused glutamate efflux from cultured dorsal spinal cord astrocytes in a time-dependent manner. 100 µmol/l MRS2179 significantly inhibited the glutamate efflux induced by ATP, which suggests that P2Y1 receptor activation is responsible for the ATP-induced glutamate efflux from astrocytes. Taken together, our results demonstrate that P2Y1 receptor plays an important role in modulating the function of astrocytes, which raises the possibility that MRS2179, a potent P2Y1-specific antagonist, may become a potential drug in treating many chronic neurological diseases characterized by astrocytic activation in the nervous system.
- Published
- 2008
28. Design and Development of a DNA Array for Rapid Detection and Genotyping of Seven Kinds of Pathogenic Microbes
- Author
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Hengbin Guo, Kai-hua Tao, Yue-xi Li, Jun Zhou, Yude Tang, Jin Zhu, Min Cao, Hui-Ying Jin, and Jin-hai Zhang
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Microbiological Techniques ,Plasmodium ,Materials science ,Genotype ,Biomedical Engineering ,Bioengineering ,Microbiology ,chemistry.chemical_compound ,Enterobacteriaceae ,Animals ,Humans ,Nanotechnology ,General Materials Science ,Vibrio cholerae ,Genotyping ,Gene ,DNA Primers ,Oligonucleotide Array Sequence Analysis ,Base Sequence ,biology ,Oligonucleotide ,Hybridization probe ,General Chemistry ,Condensed Matter Physics ,biology.organism_classification ,Molecular biology ,Hantaan virus ,Orientia tsutsugamushi ,chemistry ,Schistosoma ,Parasitology ,Leptospira interrogans ,DNA microarray ,DNA Probes ,DNA - Abstract
A DNA array for rapid detection and genotyping of the pathogenic microbes of epidemic hemorrhagic fever, tsutsugamushi disease, leptospirosis, malaria, schistosomiasis, cholera, and hemorrhagic colitis was developed. The specific and relatively conserved PCR primers and DNA probes were screened from the characteristic genes of the pathogenic microbes. The PCR or RT-PCR methods were established for amplifying and labeling the DNA fragments of the pathogenic microbes. All the probes with the same Tm value were synthesized chemically and modified with an NH2 at their 5' terminus, they were printed on glass slides for fabrication of a oligonucleotide DNA array. The developed DNA array could be used for detecting and genotyping the pathogenic microbes simultaneously, and they had a high sensitivity and specificity.
- Published
- 2005
29. Expression of an antitumor–analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli
- Author
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Li-Jun Wu, Siling Wang, Chunfu Wu, Runlin Z. Ma, Jin-Hai Zhang, Zi-Yuan Duan, and Yan-Feng Liu
- Subjects
DNA, Complementary ,Genetic Vectors ,Scorpion ,Scorpion Venoms ,Antineoplastic Agents ,Peptide ,Venom ,medicine.disease_cause ,Polymerase Chain Reaction ,Chromatography, Affinity ,law.invention ,Scorpions ,Mice ,Affinity chromatography ,law ,Complementary DNA ,biology.animal ,Escherichia coli ,Tumor Cells, Cultured ,medicine ,Animals ,Buthus ,chemistry.chemical_classification ,Analgesics ,Dose-Response Relationship, Drug ,biology ,biology.organism_classification ,Molecular biology ,Recombinant Proteins ,chemistry ,Recombinant DNA ,Electrophoresis, Polyacrylamide Gel ,Peptides ,Neoplasm Transplantation ,Biotechnology - Abstract
The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice.
- Published
- 2003
30. The Effect and Countermeasures of Introducing Electro-Technical Officer on Board
- Author
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Jin Hai Zhang, Qiang Ma, Xin Jian Liu, and Bo Chen
- Subjects
Officer ,Convention ,On board ,Engineering management ,Engineering ,Countermeasure ,Ship management ,business.industry ,General Engineering ,Computer security ,computer.software_genre ,business ,computer - Abstract
Electro-technical officer will be introduced and as a mandatory rating on board in the STCW78/10 amendments, the article introduces the origin and requirement of the ETO, analysis the impact on maritime authorities, ship management company and education and training institutions of navigation and puts forward some countermeasures, as well as soon as possible to provide the reference for our country to perform convention.
- Published
- 2012
31. [Effects of biochars produced from different sources on arsenic adsorption and desorption in soil]
- Author
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Lian-Zhu, Guan, Jing-Jing, Zhou, Yun, Zhang, Guang-Cai, Zhang, Jin-Hai, Zhang, and Zhong-Xiang, Chan
- Subjects
Manure ,China ,Biodegradation, Environmental ,Charcoal ,Animals ,Soil Pollutants ,Cattle ,Adsorption ,Arsenic - Abstract
By using OECD Guideline 106 batch equilibrium method, this paper studied the characteristics of As (V) adsorption and desorption in brown soil as affected by the biochars produced from dairy manure, pine needle, and corn straw. When the addition amount of the biochars was 0.5%, the maximum adsorption amount of As (V) was decreased in the order of dairy manure biocharpine needle biocharcorn straw biochar, which was related to the basic characteristics of the biochars. The adsorption isotherm of As (V) could be well fitted by Langmuir model (R2 = 0.997). In comparing with CK, both the adsorption capacity (lgKf = 1.99-2.10) and the adsorption intensity (1/N = 0.413-0.449) of As (V) were low, and the main adsorption mechanism was physical adsorption. The desorption rate of As (V) (14.5%-18.7%) was decreased in the order of dairy manure biocharpine needle biocharcorn straw biochar. The addition of the biochars decreased the adsorption of As (V) by brown soil, which could induce the increase of the bioavailability of As, and strengthen the toxicity of As in soil.
- Published
- 2014
32. Dendritic development of hippocampal CA1 pyramidal cells in a neonatal hypoxia-ischemia injury model
- Author
-
Yan Dong, Zhao, Shan, Ou, Sai Yu, Cheng, Zhi, Xiao, Wen Juan, He, Jin Hai, Zhang, and Huai Zhen, Ruan
- Subjects
Silver Staining ,Pyramidal Cells ,Age Factors ,Dendrites ,Functional Laterality ,Rats ,Rats, Sprague-Dawley ,Disease Models, Animal ,Animals, Newborn ,Memory ,Space Perception ,Hypoxia-Ischemia, Brain ,Exploratory Behavior ,Animals ,Corticosterone ,Maze Learning ,CA1 Region, Hippocampal - Abstract
It is believed that neonatal hypoxia-ischemia (HI) brain injury causes neuron loss and brain functional defects. However, the effect of HI brain injury on dendritic development of the remaining pyramidal cells of the hippocampus and the reaction of contralateral hippocampal neurons require further studies. The Morris water maze and Golgi-Cox staining were used to evaluate the learning and memory and dendritic morphology of pyramidal cells. The results of Golgi-Cox staining showed CA1 pyramidal neurons of HI injury models with fewer bifurcations and shorter dendrite length than the naive control group. The density of dendritic spines of hippocampal CA1 pyramidal neurons was significantly lower in the HI brain injury group than in controls. With respect to hippocampal function, the HI brain injury group presented cognitive deficits in the reference memory task and probe trail. In the HI group, the pyramidal cells of left hippocampus that did not experienced ischemia but did experience hypoxia had more complex dendrites and higher density of spine than the HI injury side and control. The functional implementation of injured hippocampus might depend mainly on the hypertrophy of contralateral hippocampus after HI brain injury. Corticosterone can partially prevent the hippocampal pyramidal cells from HI injury and reduce the difference of the bilateral hippocampus pyramidal cells, but there was no improvement in learning and memory.
- Published
- 2012
33. [Acute high altitude hypoxia causes glial activation markedly in the brain of adult rats]
- Author
-
Zhong, Yang, Jin-Hai, Zhang, and Qing-Yuan, Huang
- Subjects
Male ,Altitude ,Astrocytes ,Animals ,Brain ,Microglia ,Rats, Wistar ,Hypoxia ,Rats - Published
- 2010
34. A computing scheme of 3D Fourier migration on Graphics Processing Unit
- Author
-
Zhenxing Yao, Jin‐Hai Zhang, and Shu-Qin Wang
- Subjects
Scheme (programming language) ,Real-time computer graphics ,symbols.namesake ,Fourier transform ,Texture mapping unit ,Computer science ,Computer graphics (images) ,symbols ,Graphics processing unit ,General-purpose computing on graphics processing units ,computer ,computer.programming_language - Published
- 2009
35. Analyzing the Self-similarity of the TCP Bulk Flow
- Author
-
Xiang-Dong Liu, Jin-Hai Zhang, Jun-Xing Zhang, and Xinqin He
- Subjects
Hurst exponent ,CUBIC TCP ,Self-similarity ,business.industry ,Computer science ,ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS ,Network interface ,Topology ,TCP global synchronization ,Computer Science::Performance ,TCP Friendly Rate Control ,Network interface controller ,Flow (mathematics) ,Computer Science::Networking and Internet Architecture ,business ,Computer network - Abstract
This paper analyzed the actual TCP's bulk flow in a simple topological network with different parameters statistically, studied the self-similarity of their stable flow. The results indicated that the TCP bulk flow in 10BaseT networks is quite stable, does not present the burst and the self-similarity. But in 100BaseTx networks, the TCP bulk flow presents the burst and the self-similarity obviously, its self-similarity degree closely correlates to the communications mode and the buffer size of the network interface cards. Under the similar condition, the self-similarity of the full duplex TCP bulk flows is more significant than its of the half duplex TCP bulk flows, meanwhile, the less buffer the network interface card has, the more significant self-similarity and burst the TCP bulk traffic presents.
- Published
- 2008
36. P2Y1 receptor-mediated glutamate release from cultured dorsal spinal cord astrocytes
- Author
-
Jin-Hai Zhang, Xi-Gui Wu, Huai-Zhen Ruan, Jun-Wei Zeng, and Xiao-Hong Liu
- Subjects
Glutamic Acid ,Biology ,Biochemistry ,Synaptic Transmission ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Receptors, Purinergic P2Y1 ,Chloride Channels ,medicine ,Animals ,Calcium Signaling ,Cells, Cultured ,Chromatography, High Pressure Liquid ,Protein Kinase C ,Cell Size ,Microscopy, Confocal ,Metabotropic glutamate receptor 5 ,Receptors, Purinergic P2 ,Metabotropic glutamate receptor 6 ,Glutamate receptor ,Glutamic acid ,Thionucleotides ,Water-Electrolyte Balance ,Cell biology ,Rats ,Adenosine Diphosphate ,Posterior Horn Cells ,medicine.anatomical_structure ,Metabotropic glutamate receptor ,Astrocytes ,Neuroglia ,NMDA receptor ,Calcium ,Synaptic Vesicles ,Astrocyte - Abstract
P2 receptors have been implicated in the release of neurotransmitter and proinflammatory cytokines by the response to neuroexcitatory substances in astrocytes. In the present study, we examined the mechanisms of ADP and adenosine 5'-O-2-thiodiphosphate (ADPbetaS, ADP analogue) on glutamate release from cultured dorsal spinal cord astrocytes by using confocal laser scanning microscopy and HPLC. Immunofluorescence activity showed that P2Y(1) receptor protein is expressed in cultured astrocytes. ADP and ADPbetaS-induced [Ca(2+)](i) increase and glutamate release are mediated by P2Y(1) receptor. Ca(2+) release from IP(3)-sensitive calcium stores and protein kinase C (PKC) activation is important for glutamate release from astrocytes. Furthermore, P2Y(1) receptor-evoked glutamate release is regulated by volume-sensitive Cl(-) channels and anion co-transporter, which open up the possibility that P2Y(1) receptor activation causes the increase of cell volume. Release of glutamate by ADPbetaS was abolished by 5-nitro-2 (3-phenyl propy lamino)-benzoate plus furosemide but was unaffected by botulinum toxin A. These observations indicate that P2Y(1) receptor-evoked glutamate may be mediated via volume-sensitive Cl(-) channel but not via exocytosis of glutamate containing vesicles. We speculate that P2Y(1) receptors-evoked glutamate efflux, occurring under pathological condition, may modulate the activity of synapses in spinal cord.
- Published
- 2008
37. [Progress of studies on multidrug resistance in hematological malignancies--review]
- Author
-
Jin-Hai, Zhang and Xin-Hua, Chen
- Subjects
ATP Binding Cassette Transporter, Subfamily B ,Drug Resistance, Neoplasm ,Reverse Transcriptase Polymerase Chain Reaction ,Hematologic Neoplasms ,Antineoplastic Combined Chemotherapy Protocols ,Blotting, Western ,Humans ,Drug Resistance, Multiple - Abstract
Chemotherapy remains a major route of intervention in hematological malignancies. However, a key issue in the treatment of hematological malignancies is the development of multidrug resistance (MDR) to chemotherapeutic drugs. Several mechanisms may account for this phenomenon, including biochemical mechanisms, such as the overexpression of resistance-conferring proteins and physiological mechanisms involving the hematopoietic microenvironment. In this article the pathomechanism, diagnostic approach, interpretation of results from clinical samples and correlations with hemopoietic microenvironment were briefly reviewed. The aspects of development and problems in MDR study as well as detection methods for MDR were also discussed.
- Published
- 2006
38. [Expression, purification and serological reactivity of a chimeric antigen of GRA6 with P30 from Toxoplasma gondii]
- Author
-
Yue-Xi, Li, Jin-Hai, Zhang, Kai-Hua, Tao, and Pei-Tang, Huang
- Subjects
Immunoglobulin M ,Models, Genetic ,Pregnancy ,Recombinant Fusion Proteins ,Protozoan Proteins ,Animals ,Humans ,Antigens, Protozoan ,Electrophoresis, Polyacrylamide Gel ,Enzyme-Linked Immunosorbent Assay ,Female ,Polymerase Chain Reaction ,Toxoplasma - Abstract
Major surface protein (p30) and Dense Granule Antigen GRA6 of Toxoplasma gondii have good antigenicity, and could be used for detection of IgM against Toxoplasma gondii. GRA6 may complement P30 to reach more high sensitivity for detection of antibodies to Toxoplasma gondii, so, we try to express the chimeric protein of GRA6 and P30 by genetic engineering, identify its antignenicity and use for developing diagnosis reagent. Antigenic domains of p30 and GRA6 of Toxoplasma gondii were screened by analyzing their sequences using the software ANTHEWIN. Two DNA fragments encoding respectively antigenic domains of p30 and GRA6 were cloned, they were inserted into the same expression vector pET28a( + ) and expressed as a chimeric protein in Escherichia coli. BL21(DE3), the expressed chimeric protein of p30 with GRA6 in a form of inclusion body was about 25% of total proteins of E. coli. BL21(DE3). The inclusion body was washed once with 0.5% Triton X-100 and dissolved with 0.5% SKL, after renaturation by gradient dialysis, the recombinant protein was purified by DEAE-Sepharose FF cation column and then detected with 12% SDS-PAGE, it exists mainly in the eluted peak with 300 mmol/L NaCl and has high purity. By using enzyme-linked immunosorbent assay (ELISA), the recombinant protein was examined for reactivity with immunoglobulin M (IgM) antibodies in 6 sera from patients infected with Toxoplasma gondii ., it was reactive with all the 6 sera but not with sera from normal people, these results showed that the recombinant chimeric antigen has good antigenicity and specificity and could be used for detection of IgM against Toxoplasma gondii. The expressed chimeric protein could be used for epidemic investigation of Toxoplasma gondii, blood donor screening, especially for detection of pregnant women, and is of great significance in prevention of Toxoplasma gondii infection.
- Published
- 2005
39. [Detection of Schistosomia japonicum 5D gene by polymerase chain reaction and genechip technique]
- Author
-
Jun, Zhou, Kai-hua, Tao, Yue-xi, Li, Wan-hong, Qian, Jin-hai, Zhang, Yong, Wang, and Zhao-song, Zhang
- Subjects
China ,Genetic Techniques ,Animals ,DNA, Helminth ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Genes, Helminth ,Schistosoma japonicum - Abstract
In order to develop the diagnostic genechip for specific detection of Schistosoma japonicum (Chinese mainland strain).Probe and primers were designed based on the Schistosoma japonicum 5D gene encoding an immunogenic miracidial antigen. The probe for the conservative and specific gene sequence was spotted onto the specially treated glass slides by pin-based spotting robot Pixsys 5500 and was employed to make genechips. A polymerase chain reaction (PCR) protocol was designed to effectively amplify the 5D gene fragment containing the probe sequence from cercaria, egg, adult worm and infected Oncomelania DNA as well as other flukes DNA, respectively. After 35 cycles by PCR, the products were then labeled with fluorescent Cy3-labeled primer, using dissymmetrical PCR. The labeled PCR products of the target genes were hybridized to the diagnostic genechips for detection of Schistosoma japonicum and a fluorescent scanner (ScanArray 3000) was used to observe and record the hybridization signals.The result obtained from the study showed that a 262 bp DNA fragment was amplified from cercaria, egg and adult worm with the designed primers and enable the genechip be applied to detect a single cercaria, egg and adult worm. When the genechip was used to detect Clonorchis sinensis, Fasciolopsis busk, and Paragonimus westermani DNA, the results showed negative, indicating that the genechip had good specificity.The genchip technique for detection of Schistosoma japonicum was established successfully and having the characteristics of high sensitivity and specificity.
- Published
- 2004
40. Effect of antisense oligonucleotide of noggin on spatial learning and memory of rats
- Author
-
Xiao-Tang, Fan, Wen-Qin, Cai, Zhong, Yang, Hai-Wei, Xu, and Jin-Hai, Zhang
- Subjects
Male ,Neurons ,Proteins ,Oligonucleotides, Antisense ,Hippocampus ,Rats ,Random Allocation ,Memory ,Space Perception ,Animals ,Female ,RNA, Messenger ,Rats, Wistar ,Carrier Proteins ,Maze Learning - Abstract
To investigate the effect of antisense oligonucleotide (ASODN) of noggin on rat spatial learning and memory.Expression of noggin mRNA was measured by in situ hybridization method and the ability to spatial learning and memory was tested with Morris water maze.Compared with control rats, noggin mRNA positive neurons in dentate gyrus (DG) and CA3 region of hippocampus were markedly increased after the Morris water maze training (P0.01). The increase of noggin mRNA positive neurons in hippocampus following maze training could be significantly blocked by icv injection of antisense noggin ODN, and the injection also impaired the learning and memory formation as compared to that in control rats. But the sense oligonucleotide (SODN) had no effect.Noggin, as an embryonic gene expressed in adult hippocampus, plays an important role in the process of learning and memory formation.
- Published
- 2003
41. Subject Index Vol. 82, 2008
- Author
-
Zhuan Bian, Tiziana Fiumara, Hitesh Soni, Jalal Solati, Valeria Lucini, Shizuo Narimatsu, Lu Du, Elisabetta Miraldi, Dong Chen, Keiko Hayashi, Alessia Caronno, Bernhard J. Haas, Shinsaku Naito, Akiko Koeda, Marco Biagi, Hiroko Tonai-Kachi, Herbert Marini, Uğur Dilmen, Min Nie, Mohammad-Reza Zarrindast, Ş. Suna Oğuz, Melvin L. Billingsley, Francesca Polito, Kazem Parivar, Xi-Gui Wu, Marilou Pannacci, Dilek Dilli, Xiao-Hong Liu, Letteria Minutoli, Ming-wen Fan, Shvetank Bhatt, Alessandra Bitto, Josef Donnerer, Kenjiro Nagaoka, Wen-juan He, Jin-Hai Zhang, Harald H. Kessler, Daniela Giachetti, Francesco Scaglione, Yasuhito Taniguchi, Mukul R. Jain, Domenica Altavilla, Claude Leone, Jun-Wei Zeng, Pankaj R. Patel, Francesco Squadrito, Ajay Sharma, Mario Vaccaro, Katsuhiro Shinjo, Nobumitsu Hanioka, Huai-Zhen Ruan, Takeshi Shimizudani, and Shahrbanoo Oryan
- Subjects
Pharmacology ,Index (economics) ,Statistics ,Subject (documents) ,General Medicine ,Mathematics - Published
- 2008
42. {6,6′-Dimethoxy-2,2′-[ethane-1,2-diylbis(nitrilomethylidyne)]diphenolato}methanol-μ-nitrato-dinitratosamarium(III)nickel(II)
- Author
-
Rong-Hua Hu, Jin-Hai Zhang, Run-Qing Jiang, and Yan Sui
- Subjects
Schiff base ,Ligand ,Imine ,Inorganic chemistry ,chemistry.chemical_element ,General Chemistry ,Zinc ,Condensed Matter Physics ,Medicinal chemistry ,chemistry.chemical_compound ,Nickel ,chemistry ,Heteronuclear molecule ,Atom ,General Materials Science ,Methanol - Abstract
In the title heteronuclear ZnII–PrIII complex (systematic name: {6,6′-dimethoxy-2,2′-[ethane-1,2-diylbis(nitrilomethylidyne)]diphenolato-1κ4O1,O1′,O6,O6′:2κ4O1,N,N′,O1′}(methanol-1κO)-μ-nitrato-1:2κ2O:O′-dinitrato-1κ4O,O′-praseodymium(III)zinc(II)), [PrZn(C18H18N2O4)(NO3)3(CH4O)], with the hexadentate Schiff base compartmental ligand N,N′-bis(3-methoxysalicylidene)ethylenediamine (H2L), the Pr and Zn atoms are triply bridged by two phenolate O atoms provided by the Schiff base ligand and one nitrate. The five-coordinate Zn atom is in a square-pyramidal geometry, with the donor centers of two imine N atoms, two phenolate O atoms and one of the bridging nitrate O atoms. The PrIII center has a tenfold coordination environment of O atoms, involving the phenolate O atoms, two methoxy O atoms, one methanol O atom, and two O atoms from two nitrates and one from the bridging nitrate. Strong intermolecular O—H⋯O and some weak C—H⋯O interactions generate a two-dimensional layer structure.
- Published
- 2007
43. {μ-6,6′-Diethoxy-2,2′-[ethane-1,2-diylbis(nitrilomethylidyne)]diphenolato}trinitratocerium(III)zinc(II)
- Author
-
Yan Sui, Jin-Hai Zhang, Rong-Hua Hu, and Li-Yang Yin
- Subjects
General Materials Science ,General Chemistry ,Condensed Matter Physics - Published
- 2007
44. Comparative Study on Polarization of DNA and CdSe Quantum Dots
- Author
-
Xing-Fei, Zhou, primary, Cheng-Yi, Cui, additional, Jin-Hai, Zhang, additional, Jian-Hua, Liu, additional, and Jing-Song, Liu, additional
- Published
- 2010
- Full Text
- View/download PDF
45. Optimized finite-difference operator for broadband seismic wave modeling.
- Author
-
Jin-Hai Zhang and Zhen-Xing Yao
- Subjects
SEISMIC waves ,FINITE differences ,NUMERICAL analysis ,FREQUENCY standards ,WAVE analysis - Abstract
High-resolution image and waveform inversion of small scale targets requires the handling of high-frequency seismic wavefields. However, conventional finite-difference (FD) methods have strong numerical dispersions in the presence of high-frequency components. To reduce these numerical dispersions, we optimized the constant coefficients of the FD operator by maximizing the wavenumber coverage with- in a given error limitation. We set up three general criteria to enhance the convergence of the algorithm and reduce the optimization effort. We selected the error limitation to be 0.0001, this being the smallest in the literature, which led to perfect agreement between theoretical analyses and nu- merical experiments. The accuracy of our optimized FD methods can even reach that of much higher order unoptimized FD methods, which means great savings of computa- tional efforts and memory demand. These advantages become even more apparent with 3D modeling, especially for saving memory demand. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
46. Globally optimized finite-difference extrapolator for strongly VTI media.
- Author
-
Jin-Hai Zhang and Zhen-Xing Yao
- Subjects
EXTRAPOLATION ,FINITE differences ,MATHEMATICAL optimization ,APPROXIMATION theory ,TAYLOR'S series ,ANISOTROPY - Abstract
Implicit finite-difference (FD) migration is unconditionally stable and is popular in handling strong velocity variations, but its extension to strongly transversely anisotropic media with vertical symmetric axis media is difficult. Traditional local optimizations generate the optimized coefficients for each pair of Thomsen anisotropy parameters independently, which can degrade results substantially for large anisotropy variations and lead to a huge table. We developed an implicit FD method using the analytic Taylor-series expansion and used a global optimization scheme to improve its accuracy for wide phase angles. We first extended the number of the constant coefficients; then we relaxed the coefficient of the time-delay extrapolation term by tuning a small factor such that error is less than 0.1%. Finally, we optimized the constant coefficients using a simulated annealing algorithm by constraining that all the error functions on a fine grid of the whole anisotropic region did not exceed 0.5% simultaneously. The extended number of the constant coefficients and the relaxed coefficient greatly enhanced the flexibility of matching the dispersion relation and significantly improved the ability of handling strong anisotropy over a much wider range. Compared with traditional local optimization, our scheme does not need any table and table lookup. For each order of the FD method, only one group of optimized coefficients is enough to handle strong variations in velocity and anisotropy. More importantly, our global optimization scheme guarantees the accuracy for various possible ranges of anisotropy parameters, no matter how strong the anisotropy is. For the globally optimized second-order FD method, the accurate phase angle is up to 580, and the increase is about 18°-22°. For the globally optimized fourth-order FD method, the accurate phase angle is up to 77°, and the increase is about 22o-27°. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
47. Reducing two-way splitting error of FFD method in dual domains.
- Author
-
Jin-Hai Zhang and Zhen-Xing Yao
- Subjects
FOURIER analysis ,FINITE differences ,FOURIER transforms ,SEISMIC waves ,SEISMOLOGY - Abstract
The Fourier finite-difference (FFD) method is very popular in seismic depth migration. But its straightforward 3D extension creates two-way splitting error due to ignoring the cross terms of spatial partial derivatives. Traditional correction schemes, either in the spatial domain by the implicit finite-difference method or in the wavenumber domain by phase compensation, lead to substantially increased computational costs or numerical difficulties for strong velocity contrasts. We propose compensating the two-way splitting error in dual domains, alternately in the spatial and wavenumber domains via Fourier transform. First, we organize the expanded square-root operator in terms of two-way splitting FFD plus the usually ignored cross terms. Second, we select a group of optimized coefficients to maximize the accuracy of propagation in both inline and crossline directions without yet considering the diagonal directions. Finally, we further optimize the constant coefficient of the compensation part to further improve the overall accuracy of the operator. In implementation, the compensation terms are similar to the high-order corrections of the generalized-screen method, but their functions are to compensate the two-way splitting error rather than the expansion error. Numerical experiments show that optimized one-term compensation can achieve nearly perfect circular impulse responses and the propagation angle with less than 1% error for all azimuths is improved up to 60° from 35°. Compared with traditional single-domain methods, our scheme can handle lateral velocity variations (even for strong velocity contrasts) much more easily with only one additional Fourier transform based on the two-way splitting FFD method, which helps retain the computational efficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
48. Expression of a dominant-negative Rho-kinase promotes neurite outgrowth in a microenvironment mimicking injured central nervous system.
- Author
-
Ping YANG, Hui-zhong WEN, and Jin-hai ZHANG
- Subjects
LENTIVIRUS diseases ,CELLS ,NEURONS ,CELLULAR signal transduction ,CENTRAL nervous system ,GREEN fluorescent protein - Abstract
AbstractAim:To investigate whether lentiviral vector (LV)-mediated expression of a dominant negative mutant Rho-kinase (DNROCK) could inhibit activation of the Rho/ROCK signaling pathway and promote neurite outgrowth in a hostile microenvironment mimicking the injured central nervous system (CNS) in vitro.Methods:Lentiviral stock was produced using the three-plasmid system by transfecting HEK293 cells. Myelin prepared from rat brain was purified by two rounds of discontinuous density gradient centrifugation and osmotic disintegration. Differentiated PC12 cells and dissociated adult rat dorsal root ganglion (DRG) neurons were transduced with either LV/DNROCK or LV/green fluorescent protein (GFP) and seeded on solubilized myelin proteins. The effect of DNROCK on growth cone morphology was tested by rhodamine-conjugated phalloidin staining. Expression of DNROCK was determined by immunoblotting. The length of the longest neurite, the percentage of neurite-bearing neurons, or the total process outgrowth for all transduced neurons were measured by using the Scion image analysis program.Results:Transduction of DNROCK inhibited serum-induced stress fiber formation in NIH 3T3 cells and induced enlargement of cell bodies and decreased the phosphorylation levels of MYPT1 in HeLa cells. LV/DNROCK blocked myelin-induced increase in ROCK translocation from cytosol to membrane in LV/GFP-treated PC12 cells. DNROCK promotes neurite outgrowth of differentiated PC12 cells and DRG neurons on myelin protein. LV/DNROCK-transduced PC12 cells had longer neurites than LV/GFP-transduced cells (39.18±2.19 μm vs 29.32±1.7 μm, P<0.01) on myelin-coated coverslips. Furthermore, a significantly higher percentage of LV/DNROCK-transduced cells had extended neurites than LV/GFP-transduced cells (63.75%±8.03% vs 16.3%±3.70%, P<0.01). LV/DNROCK-transduced DRG neurons had longer neurite length (325.22±10.8 μm vs 202.47±9.3 μm, P<0.01) and more primary neurites per cell than those in LV/GFP-transduced cells plated on myelin and laminin (7.8±1.25 vs 4.84±1.45, P<0.01) or on laminin alone (5.2±1.88). LV/DNROCK-transduced cells had significantly larger growth cones (33.12±1.06 μm
2 ) than LV/GFP-pretreated cells (23.72±1.22 μm2 ).Conclusion:These results indicate that blocking the RhoA/ROCK signaling pathway by expression of DNROCK is effective in facilitating neurite outgrowth in a microenvironment mimicking injury of central nervous system. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
49. Optimized Chebyshev Fourier migration: A wide-angle dual-domain method for media with strong velocity contrasts. .
- Author
-
Jin-Hai Zhang, Wei-Min Wang, Shu-Qin Wang, and Zhen-Xing Yao
- Subjects
CHEBYSHEV polynomials ,FOURIER transforms ,ELASTIC wave propagation ,FINITE differences ,EXTRAPOLATION ,WAVE equation - Abstract
A wide-angle propagator is essential when imaging complex media with strong lateral velocity contrasts in one-way wave-equation migration. We have developed a dual domain one-way propagator using truncated Chebyshev polynomials and a globally optimized scheme. Our method increases the accuracy of the expanded square-root operator by adding two high order terms to the traditional split-step Fourier propagator. First, we approximate the square-root operator using Taylor expansion around the reference background velocity. Then, we apply the first-kind Chebyshev polynomials to economize the results of the Taylor expansion. Finally, we optimize the constant coefficients using the globally optimized scheme, which are fixed and feasible for arbitrary velocity models. Theoretical analysis and numerical experiments have demonstrated that the method has very high accuracy and exceeds the unoptimized Fourier finite-difference propagator for the entire range of practical velocity contrasts. The accurate propagation angle of the method is always about 60° under the relative error of 1% for complex media with weak, moderate, and even strong lateral velocity contrasts. The method allows us to handle wide-angle propagations and strong lateral velocity contrast simultaneously by using Fourier transform alone. Only four 2D Fourier transforms are required for each step of 3D wavefield extrapolation, and the computing cost is similar to that of the Fourier finite-difference method. Compared with the finite-difference method, our method has no two-way splitting error (i.e., azimuthal-anisotropy error) for 3D cases and almost no numerical dispersion for coarse grids. In addition, it has strong potential to be accelerated when an enhanced fast Fourier transform algorithm emerges. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
50. Accelerating 3D Fourier migration with graphics processing units.
- Author
-
Jin-Hai Zhang, Shu-Qin Wang, and Zhen-Xing Yao
- Subjects
INVARIANT wave equations ,GRAPHICS processing units ,COMPUTER software ,FOURIER transforms ,EXTRAPOLATION ,BANDWIDTHS - Abstract
Computational cost is a major factor that inhibits the practical application of 3D depth migration. We have developed a fast parallel scheme to speed up 3D wave-equation depth migration on a parallel computing device, i.e., on graphics processing units (GPUs). The third-order optimized generalized-screen propagator is used to take advantage of the built-in software implementation of the fast Fourier transform. The propagator is coded as a sequence of kernels that can be called from the computer host for each frequency component. Moving the wavefield extrapolation for each depth level to the GPUs allows handling a large 3D velocity model, but this scheme can be speeded up to a limited degree over the CPU implementation because of the low-bandwidth data transfer between host and device. We have created further speedup in this extrapolation scheme by minimizing the low-bandwidth data transfer, which is done by storing the 3D velocity model and imaged data in the device memory, and reducing half the memory demand by compressing the 3D velocity model and imaged data using integer arrays instead of float arrays. By incorporating a 2D tapered function, time-shift propagator, and scaling of the inverse Fourier transform into a compact kernel, the computation time is reduced greatly. Three-dimensional impulse responses and synthetic data examples have demonstrated that the GPU-based Fourier migration typically is 25 to 40 times faster than the CPU-based implementation. It enables us to image complex media using 3D depth migration with little concern for computational cost. The macrovelocity model can be built in a much shorter turnaround time. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
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