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Three new flavonoid glycosides, embeliaflavosides A-C (1–3), together with eight known flavonoid glycosides (4–11), were isolated from the fruits of Embelia ribes. Their structures were established...

Breast cancer is a common malignant tumor suffered predominantly by women worldwide, which results in serious levels of morbidity and mortality. To control the effects of the cancer, it is critically important to elucidate the pathophysiological processes by which it occurs and develops. Reports have demonstrated that long noncoding RNAs perform a critical role in the development and metastasis of cancers. The lncRNA TTN-AS1 is considered carcinogenic. Nevertheless, the importance and biological functions of TTN-AS1 in breast cancer require greater exploration. In the current paper, we observed that TTN-AS1 expression was significantly upregulated in breast cancer tissues/cells compared with those that are healthy. TTN-AS1 enhanced the proliferation, migration, invasion, and epithelial-mesenchymal transformation of breast cancer cells. Furthermore, a direct target of TTN-AS1, miR-139-5p was negatively regulated. In addition, zinc finger E-box binding homeobox 1 (ZEB1) is an important nuclear transcription factor, the expression of which is increased in multiple tumors. Here, we also found that ZEB1 is a target of miR-139-5p, of which TTN-AS1 could regulate the expression through competition with miR-139-5p. That is, TTN-AS1 promoted proliferation and invasion of breast cancer cells by interaction with the miR-139-5p/ZEB1 axis. In conclusion, the present study aimed to illustrate the significance of TTN-AS1 in breast cancer metastasis and contribute to potentially innovative strategies for its treatment.

Three new flavonoid glycosides, embeliaflavosides A-C (

Pancreatic ductal adenocarcinoma (PDAC), a common malignancy originated from the digestive system worldwide, has a poor clinical outcome. SPOCK1 is a widely investigated member of the Ca2+ -binding proteoglycan family and functions as an essential driver in several cancers. However, the complex regulatory role of SPOCK1 in PDAC is unclear. Bioinformatics analysis predicted an interrelationship between increased SPOCK1 expression and the clinical characteristics of patients with PDAC. The SPOCK1 expression levels in fresh tissue samples were confirmed, and SPOCK1 expression was then knocked down by lentivirus-mediated short hairpin RNA. Cell proliferation, metastasis, and apoptosis were detected through Cell Counting Kit-8, colony formation assays, invasion and migration assays, flow cytometric analysis, quantitative real-time polymerase chain reaction, and Western blot experiment. On the basis of the Cancer Genome Atlas database, we found a significantly higher level of SPOCK1 in PDAC than in adjacent nontumor tissues. Patients with PDAC with high SPOCK1 expression exhibited shorter overall survival time, as well as disease-free survival time. The knockdown of SPOCK1 significantly decreased the proliferation and metastasis of PCNA-1 and MIA PaCa-2 cells. Moreover, the knockdown of SPOCK1 led to cell cycle arrest in G0/G1 phase and increased the proportion of apoptotic PDAC cells by regulating members of the caspase and Bcl-2 families. Our data proved that SPOCK1 is a critical regulator of tumor proliferation and metastasis in PDAC cells. Therefore, SPOCK1 might be a potential prognostic and therapeutic target molecule in PDAC.

Diabetic retinopathy is the main ocular complication of diabetes mellitus. The aim of this study was to investigate the protective effect and mechanism of resolvin D2 (RvD2) on diabetic retinopathy. Streptozocin-induced C57/BJ diabetic mice were divided into three groups: normal control, diabetes mellitus, and diabetes plus RvD2 treatment. After three months of diabetic model induction, exogenous RvD2 was injected, monthly for three months, into the vitreous cavity of mice in the diabetic treatment group. Retinal vascular leakage, ganglion cell apoptosis, inflammatory factor expression, and oxidative stress factors were detected one month after the last injection. The levels of retinal vascular leakage and ganglion cell apoptosis in diabetic mice treated with RvD2 were significantly lower than those in untreated diabetic mice, as were the retinal levels of inflammatory factors and oxidative stress. In conclusion, RvD2 might be used as a retinal protective factor for diabetes mellitus by reducing inflammation and oxidative stress.

With people's changing life style and dietary habits, the prevalence of type 2 diabetes mellitus (T2DM) has become much more serious than ever before. T2DM is a polygenic metabolic disorder, resulting from the interaction of genetic and various environmental factors. Among all the T2DM related genes, the transcription factor 7 like 2 (TCF7L2) gene is one of the most relevant risk-related genes for T2DM. However, the role of TCF7L2 in T2DM pathogenesis has not yet been interpreted thoroughly. Based on the experimental studies in recent years, this review discusses several possible mechanisms of T2DM pathogenesis induced by TCF7L2.

Nine new alkylresorcinols, designated as embelialkylresorcinols A-I (1–9), along with five known compounds (10–14) were isolated from the fruits of Embelia ribes. Their structures were elucidated by extensive spectroscopic analysis (1D, 2D NMR and HRESIMS), optical rotation data and modified Mosher method. Notably, embelialkylresorcinols A-H (1–8), possessing double aromatic rings linked with an aliphatic chain, are reported from the genus of Embelia (Myrsinaceae) for the first time. Most of the isolated compounds were evaluated for cytotoxic activity, and the results indicated that compounds 3, 5, 6 and 8 displayed moderate cytotoxicity against three human cancer cell lines (Hep3B, A549 and HCC1806) with IC50 values ranging from 23.06 to 41.49 μM.

Two new 8- O -4′ neolignan glycosides, named matteustruthiosides A ( 1 ) and B ( 2 ) were isolated from the aerial parts of Matteuccia struthiopteris . Their chemical structures were characterized on the basis of various spectroscopic techniques. The absolute configurations of them were determined by experimental circular dichroism (CD) spectra. Compounds 1 and 2 were inactive against influenza A (H1N1) virus in vitro .

In the civil aviation industry, the number of flights and the number of airports have been increasing. Airlines recommendation service which to provide customers with more efficient and shorter routes has become one of the effective measures for improving the service quality of aviation. Actually, Airports and air-lines can be formed a weighted directed graph (for example, we use plane fares to represent the weights on the edges of the graph, and represent the vertices in the graph by the airports), and Airlines recommendation is to help user to find out the shortest path from the source vertex to the target vertices of the weighted directed graph. The shortest path search can be done by using the Dijkstra incremental optimization algorithm[1]. However, as the incremental part become larger and larger, the time spent on shortest path computation will be growing rapidly. In order to shorten the computing time, this paper proposes a dynamic SPT increment algorithm based on Hadoop platform. By the Hadoop efficient distributed platform[2], using MapReduce to take a dynamic search for the incremental part of the SPT. Finally, regenerates the new shortest path tree to improve the algorithm efficiency and performance.

Power loss is a serious problem for all power companies. To find effective means of abnormal electricity iidentification is a popular research field in recent years. This paper puts forward an anomalous electricity use model based on multi-dimensional compound features of electricity users. The support vector machine, local outlier factor, correlation measurement based on the similar user power load, and correlation change rate measurement based on the most relevant users—these four algorithms are adopted to extract four-dimensional compound features of anomalous electricity use from the perspective of global anomaly, local anomaly, regional space, and time sequence. Next, the logistic regression (LR) model is trained based on the compound features. After training, the LR model is adopted as the final anomalous electricity use identification model. Analysis of the practical power load data of users suggests that the LR model combine respective advantages of the four-dimensional compound features. Detection of anomalies using the LR model is an effective approach, which can reliably and accurately identify residents’ anomalous electricity use. From the accuracy rate, recall rate, precision rate and scores of F1, it can be seen that the LR model is significantly superior to SVM.

Jun activation domain-binding protein 1 (Jab1) was overexpressed in breast cancer, which was involved in degradation of the cyclin-dependent kinase inhibitor p27(Kip1). The objective of this study was to examine the effect of brain specific kinase 1 (BRSK1) expression on Jab1 over-expression and related signaling pathway in breast cancer.Immunohistochemical analysis was performed in 95 human breast carcinoma samples and the data were correlated with clinicopathologic features. Furthermore, Western blot analysis was performed for BRSK1 and Jab1 in breast carcinoma samples and cell lines to evaluate their protein levels and molecular interaction.We found that the cytoplasmic BRSK1 expression was inversely associated with Jab1 expression (P0.01) and correlated significantly with histologic grade (P=0.006), however nuclear BRSK1 expression couldn't obtain similar results. Kaplan-Meier analysis revealed that survival curves of low versus high expressers of cytoplasmic BRSK1 and Jab1 showed a highly significant separation in breast cancer (P0.01). While in vitro, following release of breast cancer cell lines from serum starvation, the expression of Jab1, phosphor-Akt (p-Akt) was up-regulated, whereas BRSK1 and p27(Kip1) were decreased. Treatment of phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 could diminish Jab1 expression but increase BRSK1 expression. In addition, we employed siRNA technique to knock down Jab1 and/or BRSK1 expression and observed their effects on MDA-MB-231 cell growth.BRSK1 is a novel tumor suppressor in breast cancer which inversely correlated with Jab1 expression, may involve in the restoring Jab1-induced suppression of p27(Kip1) and may regulate cell cycle through the PI3K/Akt pathway.

The adaptive fuzzy logic systems are constructed in this paper by utilizing the data information sampled from the inputs and outputs of unknown functions in the nonlinear systems controlled, and then output stable controller is synthesized for a class of uncertain nonlinear systems based on the universal approximation property of adaptive fuzzy logic systems. Finally, the simulation shows the validity of the method in this paper.

One new flavanonol, demethylmatteucinol (1), and nine new flavanone glucoside derivatives, matteflavosides H-J (2–4) and matteuinterates A-F (5–10), were isolated from the rhizomes of Matteuccia intermedia C.Chr., along with 21 known flavanones (11−31). Notably, all of them contain C-methylation in the A-ring. The structures of the compounds were elucidated by spectroscopic methods and chemical derivatization. The α-glycosidase inhibition assay indicated that compounds 12–17 showed potent inhibitory activity with IC50 values of 12.4–69.7 μM, which suggested their hypoglycemic effect.

Ski-interacting protein (SKIP) is a nuclear hormone receptor-interacting cofactor, interactions with the proto-oncogene Ski, appears to modulate a number of signalling pathways involved in control of cell proliferation and differentiation, and may play a critical role in oncogenesis. In the present study, to investigate the potential roles of SKIP in breast cancer, expression patterns, interaction and the correlation with clinical/prognostic factors of SKIP and Ki-67 were examined among patients with breast cancer. Immunohistochemistry and Western blot analysis were performed for SKIP in 85 breast carcinoma samples. The data were correlated with clinicopathological features. The univariate and multivariate survival analyses were also performed to determine their prognostic significance. We found that SKIP was over expressed in breast carcinoma as compared with the adjacent normal tissues. High expression of SKIP was positively associated with histological grade (P = 0.01) and Ki-67 (P = 0.004). Univariate analysis showed that SKIP expression was associated with a poor prognosis (P = 0.006). While in vitro, following release of breast cancer cell lines from serum starvation, the expression of SKIP was up-regulated, whereas p27 was down-regulated. In addition, we employed small interfering RNA (siRNA) technique to knock down SKIP expression and observed it effects on MDA-MB-231 cells growth. SKIP depletion by siRNA inhibited cell proliferation, blocked S phase and decreased cyclin A and cyclin B levels. On the basis of these results, we suggested that SKIP overexpression was involved in the pathogenesis of breast cancer, which might serve as a future target for breast cancer.

This paper puts forward a designing scheme of remote monitoring system for parameters of water quality based on GPRS, which is mainly try to improve the existing problems in traditional method such as low monitor frequency, more error in sampling, much dispersion in data. After carefully study the communication principle of GPRS, the monitoring information platform is constructed by adopting chip S3C2440A as key of hardware circuit ,making use of sensor to sample data in real-time, and with public GPRS as data channel. The test turns out that the system works well in function of wireless data channel and data analysis, moreover, it has good property in expansibility for other wireless T&C system.

isolation, structural elucidation, and anti-influenza A (H1N1) activity of matteustruthiosides A (I) and B (II)

Adenylate cyclase-associated protein 1 (CAP1) is a conserved protein that was found to be up-regulated in breast cancer and related to the migration of breast cancer. We verified its roles in breast cancer specimens and cell lines. In our results, 71 of 100 specimens of breast cancer showed high levels of CAP1 by immunohistochemistry. Associated with statistical analysis, we saw that CAP1 was related to the grade of breast cancer. In MDA-MB-231, the expression of CAP1 was the highest and by knocking down the expression of CAP1 in MDA-MB-231, its ability for proliferating and migrating apparently decreased and induced changes in morphology, which were related to the arrangement of F-actin. Therefore, CAP1 might be a potential molecular targeted therapy for surgery and immune treatment.