Search

In order to study the differences in the comprehensive quality and processing characteristics of different varieties of wampee (Clausena lansium (Lour.) Skeels), seven kinds of wampee ('Bingtanghuangpi' 'Jixinhuangpi' 'Jinhuangpi' 'Guifeisanhao' 'Baimihuangpi' 'Huananyihao' 'Wuhehuangpi') were taken as the research objects, the physical property, chemical composition and antioxidant capacity of the fruits were analyzed, and the processing characteristics of the seven kinds of wampee were evaluated and categorized using principal component analysis and cluster analysis. The results showed that the pulping rate, soluble sugar and organic acid content of seven kinds of wampee ranged from 50.51% to 60.71%, 18.93 to 48.91 mg/g and 8.13 to 14.00 mg/g. Respectively, the 'Bingtanghuangpi' had the highest pulping rate, the 'Baimihuangpi' had the highest soluble sugar content, the 'Jixinhuangpi' had the highest organic acid content. The total phenol content of pericarp, total phenol content of fruit pulp, total flavonoid content of pericarp and total flavonoid content of fruit pulp ranged from 2446.4 to 4045.5 mg/kg DW, 763.7 to 118.2 mg/kg, 234.1 to 678.7 mg/kg DW, and 96.8 to 143.4 mg/kg. Respectively, the 'Guifeisanhao' had the highest total phenolic and total flavonoid content, and the strongest antioxidant capacity (ferric reducing antioxidant power (FRAP), DPPH radical scavenging capacity). Nine phenolic compounds in wampee were identified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and rutin was the main phenolic compound. The results of principal component analysis showed that 25 indicators reflecting the quality of different varieties of wampee, that can be expressed by four principal components (the cumulative contribution rate reached 87.51%), of which 'Guifeisanhao' scored the highest in the comprehensive evaluation of principal component analysis, and 'Bingtanghuangpi' scored the lowest. The results of cluster analysis were basically consistent with the results of principal component analysis. Overall, 'Guifeisanhao' and 'Wuhehuangpi' had the highest content of total phenolics, total flavonoids and the strongest antioxidant ability, but the soluble sugar content was relatively low, which was more suitable for the extraction of active substances. 'Bingtanghuangpi' and 'Baimihuangpi' had high soluble sugar content and low organic acid content, with moderate sweet and sour taste, which was suitable for fresh food, and the high organic acid content of 'Jinhuangpi' 'Huananyihao' and 'Jixinhuangpi' was more suitable for processing into food ingredient.

The spilling-breaking waves that appear in chirped wave packets are studied in a two-dimensional wave channel. These waves are produced by superposing waves with gradually decreasing frequencies. The analysis focuses on the nonlinear characteristics, energy variation, and energy transformation during the evolution and breaking of wave groups. Ensemble empirical mode decomposition is used to analyze the non-breaking and breaking energy variations of the intrinsic mode functions (IMFs). It is found that the third-order IMF component is a source of non-breaking energy dissipation and the second-order IMF, which represents a short wave group with a relatively higher energy content, is a primary source of the energy loss caused by wave breaking. Additionally, the findings reveal that among the three waves preceding the maximum crest, the wave closest to the maximum crest carried most of the energy. When wave breaking occurs, the energy dissipation caused by the wave breaking primarily originates from that wave. After wave breaking, whether it is the first breaker or subsequent breakers, the main energy dissipation occurs in a frequency range higher than the dominant frequency. This energy loss plays a significant role in increasing the energy of free waves. Moreover, a potential link between the number of carrier waves and wave breaking phenomena has been found. As the number of carrier waves increased, both the nonbreaking and breaking energy dissipation rates exhibited an overall increasing trend. The amount of nonbreaking energy dissipation was generally more than twice the breaking energy dissipation rate. For wave groups with more carrier waves, the modulation instability plays a significant role in generating larger waves. Furthermore, an analysis of the dominant frequency variations of the wave group before wave breaking suggests that wave breaking is not a sufficient condition for a frequency downshift in the wave spectra.

Abstract To ensure bridge safety, the flood risk analysis is significant important. However, due to the small size and large number of piers in the short-distance multi-bridge system, the extremely long calculation time and low efficiency of the numerical model are induced by the small mesh size and large mesh number. In this paper, a flood risk assessment model of the multi-bridge system with short distance was established to improve the calculation efficiency based on the finite volume method combined with the Delaunay mesh adaptive refinement method. The calculated water level with refined and non-refined mesh was compared with the experimental data of a partial failure dam break test case and Shukry experiment of open channel bend flow. The calculated water level results are in good agreement with the experimental data. In addition, the mesh refinement model improved the calculation efficiency by more than 73% with ensuring the calculation accuracy. Finally, the flood risk of a real multi-bridge system with short distance was evaluated by using the numerical model. The calculated results shown that, different from the general flow law, the water level in the upstream and downstream channel of Bridge 2 rose with a maximum difference value of 0.326 m while the water level in the far downstream channel of Bridge 2 dropped result from the construction of Bridge 2 on the basis of the Bridges 1, 3 and 4. The construction of Bridge 2 also increased the flow velocity around Bridge 3 with maximum 0.013 m/s. This study provides a new tool and technical reference for flood risk analyses of similar multi-bridge system with short distance.

Due to natural and anthropogenic disturbances, natural gas hydrates with morphologies of nodules and chunks dissociate and release massive free gas, creating large cavities within fine-grained marine sediments. However, it is still a challenge to quantify the impact of gas cavities on mechanical properties of cavitied fine-grained marine sediments as there is a lack of efforts focusing on the inner structure visualization. In this study, an oedometer test and X-ray computed tomography scans are jointly conducted on marine clayey silt with gas cavities, and the confined compressibility as well as the inner structure change under an undrained condition are explored, followed by development of a theoretical model depicting the void ratio change. The results show that vertical loading induces a void ratio reduction, and the reduced void ratio can fully recover after being unloaded. Although being fully recovered, unrecovered changes of the inner structure still remain after being unloaded. Examples include closed cracks in the lower matrix, new occurring cracks in the upper matrix, and the fragmented gas cavity. In addition, the void ratio linearly increases with the increasing inverse of normalized pore gas pressure, while the coefficient of the effective stress linearly decreases with the increasing inverse of normalized vertical loading stress. The proposed theoretical model captures the essential physics behind undrained confined deformation of fine-grained marine sediments with gas cavities when subjected to loading and unloading.

Due to significant influence on the safety of marine structures, the interaction between extreme waves and structures is a crucial area of study in marine science. This paper focus on the verification of a solitary wave meshless SPH model and the application of the model on the interaction between solitary waves and semi-submersible structures. A solitary wave propagation model is established based on the SPH method combined with Rayleigh solitary wave theory, quintic kernel function, artificial viscosity, and Symplectic Method. The accuracy of the model is validated by comparing the calculated wave height with the theoretical value. The calculated results with relative particle spacing H0/d0 ≥ 20 are in good agreement with the analytical solution. The simulated solitary wave is also quite stable with a maximum L2 error 0.016. Therefore, the proposed SPH model can accurately simulate the propagation of the solitary waves. A case study on the interaction between solitary waves and semi-submersible platforms is conducted. The results show that the interaction between solitary waves and semi-submersible causes two double peaks with wave heights of 0.398 m and 0.410 m, respectively, induced by overtopping at the center of the platform. The wave transmission coefficient Kt is 0.880 due to that the solitary wave height reduces from 0.498 m to 0.438 m after the solitary wave propagates through the semi-submersible structure. In addition, the solitary wave induces significant vertical wave loads of the structure with a load amplitude of 0.688, while horizontal wave loads are relatively small with a load amplitude of 0.089. The solitary wave arrived the structure induces the upstream and downstream overtopping and forms a hydraulic jump leading to the complex flow field. The maximum velocity at the top and bottom of the structure is 2.2 m/s and 0.8 m/s respectively. Positive or negative vortex are formed at the bottom of the leading edge, top and downstream of the structure with the maximum intensity 28 s-1 and -40 s-1. In a word, the meshless SPH model can conveniently and accurately simulate the propagation of the solitary waves, and be applied to the investigation of the wave height, velocity, vorticity, wave load, and wave breaking of the interaction between solitary waves and structures in ocean engineering.

Abstract The colonization of bacterial pathogens is a major concern in wound infection and becoming a public health issue. Herein, a core–shell structured Ag@MSN (silver core embedded with mesoporous silica, AM)-based nanoplatform was elaborately fabricated to co-load ciprofloxacin (CFL) and tumor necrosis factor-α (TNF-α) small interfering RNA (siTNF-α) (AMPC@siTNF-α) for treating the bacterial-infected wound. The growth of bacterial pathogens was mostly inhibited by released silver ions (Ag+) and CFL from AMPC@siTNF-α. Meanwhile, the loaded siTNF-α was internalized by macrophage cells, which silenced the expression of TNF-α (a pro-inflammatory cytokine) in macrophage cells and accelerated the wound healing process by reducing inflammation response. In the in vivo wound model, the Escherichia coli (E. coli)-infected wound in mice almost completely disappeared after treatment with AMPC@siTNF-α, and no suppuration symptom was observed during the course of the treatment. Importantly, this nanoplatform had negligible side effects both in vitro and in vivo. Taken together, this study strongly demonstrates the promising potential of AMPC@siTNF-α as a synergistic therapeutic agent for clinical wound infections. Graphical Abstract

Abstract Background Betanodaviruses, members of the Nodaviridae family, are the causative agents of viral nervous necrosis in fish, resulting in great economic losses worldwide. Methods In this study, we isolated a virus strain named seahorse nervous necrosis virus (SHNNV) from cultured big-belly seahorses Hippocampus abdominalis in Xiamen city, Fujian Province, China. Virus isolation, PCR detection, phylogenetic analysis, qRT-PCR, fluorescence in situ hybridization and histology were used for virus identification and analysis of virus histopathology. Furthermore, an artificial infection experiment was conducted for virulence testing. Results Brain and eye tissue homogenates of diseased big-belly seahorses were inoculated onto a grouper spleen (GS) cell monolayer at 28 °C. Tissue homogenates induced obvious cytopathic effects in GS cells. PCR and sequencing analyses revealed that the virus belonged to Betanodavirus and shared high sequence identity with red-spotted grouper nervous necrosis virus isolates. qRT-PCR and fluorescence in situ hybridization revealed that SHNNV mainly attacked the brain and eye. Histopathological examination revealed that the virus led to cytoplasmic vacuolation in the brain and retinal tissues. Infection experiments confirmed that SHNNV was highly infectious, causing massive death in big-belly seahorses. Conclusion A novel seahorse betanodavirus from the big-belly seahorse cultured in China was discovered. This finding will contribute to the development of efficient strategies for disease management in aquaculture.

The effect of the non-uniform bathymetry on the nonlinear wave dynamics for the freak wave is investigated experimentally, with emphasis on the interrelations between different nonlinear behaviors resulting from various geometric parameters and spectral analysis. Both the frequency modulation and the nonlinear phase coupling can be provoked by the decreasing water depth and weakened after the top peak of the bar, the nonlinear exhibition for transferring energy to high-frequency contents over shoal supports that frequency modulation can reflect nonlinear phase coupling well. The consistent change of the Hilbert energy spectrum and the bicoherence shows that the main nonlinear interaction in the process of wave propagation in shallowing water is quadratic nonlinearity. In addition, the geometric study is conducted to investigate the effect of the water depth on the parametric variations, the research results show that the mean asymmetry and kurtosis change abruptly when the wave approaches the top peak of the bar. As the wave propagates along the water flume, freak waves can be generated at various locations, however, they appear more frequently as waves propagate close to the shallowest water depth, and the maximum probability of occurrence for a freak wave can be up to about 1%.

Abstract Background Increased glucose fluctuation has been related to poor prognosis in patients with critical illnesses, while its prognostic role in patients with acute stroke remains unknown. The meta-analysis aimed to evaluate the association between the acute glycemic variation (GV) and mortality risk in patients with acute stroke. Methods Cohort studies were obtained by searching Medline, Web of Science, Embase, Wanfang and CNKI databases. A random-effect model which incorporates the intra-study heterogeneity was chosen to pool the results. Results Ten cohort studies with 1433 patients were included, and 280 (19.5%) of them died within 90 days of disease onset. Results of the meta-analyses showed that a higher acute GV was associated with an increased risk of early mortality in patients with acute stroke, as indicated by GV measured with the coefficient of variation of blood glucose (CVBG, odds ratio [OR]: 2.24, 95% CI 1.40 to 3.58, p 0.05). Conclusions Higher acute GV may be an independent risk factor of early mortality in patients with acute stroke.

Hydraulic jumps are a rapid transition from supercritical to subcritical flow and generally occur in rivers or spillways. Owing to the high energy dissipation rate, hydraulic jumps are widely applied as energy dissipators in hydraulic projects. To achieve efficient and accurate simulations of 2D hydraulic jumps in open channels, a parallel Weakly Compressible Smoothed Particle Hydrodynamics model (WCSPH) with Shepard Density filter was established in this study. The acceleration of the model was obtained by OpenMP to reduce execution time. To further reduce execution time, a suitable and efficient scheduling strategy was selected for the parallel numerical model by comparing parallel speed-ups under different scheduling strategies in OpenMP. Following this, two test cases of uniform flow in open channels and hydraulic jumps with different inflow conditions were investigated to validate the model. The comparison of the water depth and velocity fields between the numerical results and the analytical solution generally showed good agreement, although there was a minor discrepancy in conjugate water depths. The numerical results showed free surface undulation with decreasing amplitude, which is more consistent with physical reality, with a low inflow Froude number. Simultaneously, the Shepard filter was able to smooth the pressure fields of the hydraulic jumps with a high inflow Froude number. Moreover, the parallel speed-up was generally able to reach theoretical maximum acceleration by analyzing the performance of the model according to different particle numbers.

By neglecting the viscosity of fluid and rotation in flow, the theory of potential flow cannot accurately predict the hydrodynamic forces on the structures under significant viscous effects. In this study, the effects of liquid viscosity on the hydrodynamic forces on a horizontal circular cylinder underwater with a large-amplitude forced oscillation were investigated. The study used a two-dimensional two-phase flow wave tank model based on the viscous fluid theory using the OpenFOAM® package. The numerical calculations were carried out under different types of liquid (i.e., liquid with different viscosities). The liquid viscosity effects are visually shown by comparison of the various frequency components of the hydrodynamic forces on the cylinder, and the magnitude and phase relations of the viscous shear forces and the pressure forces. By analyzing the distribution characteristics of the flow fields around the circular cylinder, the viscous-effect mechanisms are revealed. It is found that the discrepancies of the contributions of viscous shear forces, and the discrepancies of the vortex effects on the phase and magnitude of the pressure forces lead to the obvious differences among the results under different liquid viscosities.

Characteristics of hydrodynamic forces and flow fields around the semi-submersible platform induced by the internal solitary wave (ISW) propagation were investigated in a 3D numerical wave tank. Good agreements between numerical results and experimental data of forces and torque on the platform were achieved. The hydrodynamic loads increased and decreased with the increases in the ISW amplitude and fluid depth ratio, respectively. The pressure mainly contributed to the force on the platform. The horizontal forces on bracings were negligible. Almost all the vertical forces on the platform were derived from those on pontoons. The horizontal force and torque on the platform increased with the increases in the angle between the platform symmetrical axis and the ISW propagation direction. The platform subjected the maximum vertical force when the angle was 0°. There were obvious velocity reductions around the platform during the ISW propagation, as visible vortexes shedding around the platform could be observed. Complexities of the distributions of flow fields around the platforms located at the 30°- and 60°-direction were greater than those around the platforms located at the 0°- and 90°-direction. Flow fields around the same kind components of each platform located at different angles were similar.

The success or failure of river closure is directly related to the construction period and project benefit. Therefore, it is very necessary to study the river closure by an appropriate method. In this paper, a 1D−2D coupled river closure model is established to optimize the closure flow rate, closure period, and layout of a real closure project. The 1D transition model between open channel flow and pressurized flow is established by a finite volume scheme. For the 2D model, 2D shallow water equations are solved using an unstructured finite volume scheme. The 1D model and 2D model are coupled by considering the mass and momentum conservation. To validate the model, a physical experiment of a real river closure project is set up according to the gravity similarity criterion with a scale of 1:80. Then, the experimental data obtained by the calibrated physical experiment is compared with the numerical results. Good agreements are achieved in terms of surface elevation, velocity, and flow rate. Finally, the real river closure project is further investigated by the model. The layout, closure flow rate and closure period of this project is analyzed and optimized. The original design of the berm is more suitable to discharge the flow. Moreover, the first stage cofferdam should be removed to floor elevation upstream and downstream of the dam. The river closure flow rate should not exceed 2380 m3/s.

PURPOSE:High expression of highly upregulated in iver cancer (HULC) has been found associated with increased metastasis and poor prognosis with cancer. This meta-analysis aimed to determine the pooled effect of HULC on metastasis and prognosis of cancers. METHOD:The studies were accessed using multiple databases. RevMan5.3 and STATA14.0 were used to estimate pooled effects, the heterogeneity among studies, and publication bias for the association of HULC and overall survival (OS). RESULTS:A total of 9 studies of 966 cancer patients were included. Risk of lymph node metastasis was increased with high versus low HULC expression (pooled odds ratio [OR] = 4.83, 95% confidence interval [CI] 1.59-14.63) as was distant metastasis (pooled OR = 5.44, 95% CI 2.33-12.74). Furthermore, OS time was shortened with high HULC expression (pooled hazard ratio [HR] = 1.48, 95% CI 1.03-2.12), especially in Chinese patients (pooled HR = 2.04, 95% CI 1.55-2.68), and risk of recurrence was increased (pooled OR = 6.68, 95% CI 2.77-16.13). CONCLUSION:HULC might be a potential biomarker for therapy and prognosis surveillance in cancers.

Purpose: Abnormal expression of miR-223 in cancerous tissue has confirmed it as an important player in tumorigenesis of cancers, such as hepatocellular carcinoma, colorectal carcinoma, osteosarcoma, gastric cancer, and chronic lymphocytic leukemia. The present meta-analysis aimed to explore the association between circulating miR-223 and prognosis of cancers. Methods: The studies were accessed by an electronic search of multiple databases. RevMan5.3 and STATA14.0 were used to estimate the heterogeneity among studies, pooled effects, and publication bias. Results: Ten studies with data of 1,002 patients with cancer were included in this meta-analysis. The risk of metastasis from stages 3 to 4 of TNM did not decrease when high versus low circulating expression of miR-223 were compared (pooled odds ratio =0.50, 95% CI: 0.24-1.03). In case of prognosis, the overall survival time was not significantly longer with high circulating miR-223 expression (pooled hazard ratio [HR] =0.64, 95% CI: 0.38-1.11) in all cancer types. However, the overall survival time of chronic lymphocytic leukemia (pooled HR =0.19, 95% CI: 0.07-0.54) increased in subgroup analysis. Moreover, the treatment-free survival of chronic lymphocytic leukemia was significantly increased with high circulating miR-223 expression (pooled HR =0.38, 95% CI: 0.23-0.64). Conclusion: Circulating miR-223 was not an effective biomarker in prognosis surveillance in all cancers but in chronic lymphocytic leukemia. [ABSTRACT FROM AUTHOR]