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This study analyzed the role of G1 to S phase transition 1 protein (GSPT1) in promoting progression of liver cancer cells.A bioinformatics database was used to analyze the expression levels of GSPT1 in liver cancer tissues and the prognosis of patients. Subsequently, Western blotting and quantitative PCR were used to verify the expression levels of GSPT1 between normal hepatocytes and hepatoma cells. We used a CRISPR/Cas9 system to construct knockouts of GSPT1 in HepG2 and HCCLM9 liver cancer cells. The effect of GSPT1 on liver cancer cell migration and invasion was analyzed using flow cytometry, migration, and tumor formation assays.The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset indicated that GSPT1 expression was upregulated in liver cancer cell lines, and patients with liver cancer had poor prognosis. Knockout of GSPT1 in cells significantly inhibited tumor proliferation, cell migration, and growth in vivo.In this study, we found that GSPT1 promotes the migration and invasion of liver cancer cells.

Ti-6Al-4V (wt%) alloy samples with dog-bone and box shapes respectively were fabricated by selective laser melting (SLM). The microstructures and mechanical properties of the 3D printed Ti-6Al-4V samples with and without heat treatment were characterized and tested. The microstructures of the as-fabricated dog-bone shaped samples were mainly composed of acicular α’ phase. After annealing at 700°C, the acicular α’ phase changed into an α/β lamellar structure. After solution treatment at 955°C, water quenching and aging at 550°C, the microstructure was mainly composed of primary α phase and α/β lamellar structure. The optimum heat treatment is annealing, and the mechanical properties of the annealed sample are as follows: yield strength: 1015 MPa, ultimate tensile strength (UTS): 1083 MPa and elongation to fracture: 7.9%. The microstructures of the box-shaped samples after annealing mainly consist of α phase and α/β lamellar structure. When stretched along the direction parallel to the crystal growth direction, the yield strength and UTS of the sample are 1054 and 1090 MPa，and its elongation to fracture is 6.3%. When stretched along the direction perpendicular to the crystal growth direction, the yield strength and UTS of the sample are 1019 and 1068 MPa respectively, and its elongation to fracture is 8.7%.

The modal of large flexible structure with low and close modes hardly computes accurate data in the ground. While binocular stereo vision is valued for non-contact measurement, greater flexibility and higher precision in on-orbit identification method. This paper optimized the binocular vision algorithm. The difference between original and new algorithm, which includes the aiming-point number, the identification time and the memory number was shown. The conclusion manifests that new algorithm can improve the identification accuracy and speed up the identification and provide support for on-orbit modal identification.

The vehicle may vibrate when it moves under or exceeding water. This vibration will interact with the flow filed around to form a fluid-structure interactiondynamics system. In this paper, the numerical simulation of a cylinder in the process of moving under or exceeding water with ABAQUS and FLUENT is presented. Simultaneously, the first three damping ratio of the system are obtained by damping identification. For different density, viscosity and velocity of water, model separately to get the impact of each factor. Besides, the continuous process of exceeding water has been discretized to obtaindifferent oscillating curvesat different positions with respect to the water,and then the change of the damping ratio is presented. Thus the damping characteristics of the system in the water-exit process have been obtained approximately.

In the launching stage, the vehicle runs from the tube into the water above. This is a nonlinear and time-varying coupling process of water, structure and gas. This article aims at the dynamic response of the underwater vehicle in the vertical tube-exit stage. According to experimental pressure values on measuring points, the hydrodynamic pressure distribution on the vehicles surface is determined by Cosine Hypothesis. By LS-DYNA, the vehicles FEM model is built, and the hydrodynamic pressure is applied on the model. With numerical simulation, its dynamic response is obtained.

In this paper, two kinds of time varying parameter identification methods which are called identification method of using broken line to approximate time varying parameter and identification algorithm with auto-regulation forgetting factor are studied. The two methods are used for short-term time-varying system parameter identification in simulations. According to the results of the simulations, the applicable conditions of the two kinds of identification methods are analyzed. The results of the simulations indicate the effectiveness of the two methods. Furthermore whether the two kinds of identification methods are sensitive to noise or not is studied by setting different noise levels in the simulations. Finally, experiments of a variable mass cylindrical shell are adopted to demonstrate the efficiency of the two kinds of time varying parameter identification methods.

To identify a novel HLA allele in Chinese population.A new HLA-B allele was initially detected by an usual PCR-SSP and PCR-SSOP in routine typing HLA allele. Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele.The HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B 4409 allele at nt538(GC), nt539(AT) and nt540 (CG) in exon 3, resulting in an amino acid change from D(GAC) to L(CTG) at codon 180.A novel HLA allele was confirmed by the SBT and it was officially designated as HLA-B 4446 by WHO Nomenclature Committee for Factors of the HLA System in September,2005.