Search

Quantitative trading decision models have a key influence on financial investment. Firstly, this study established an LSTM model by using long-term and short-term memory networks and predicted the future prices of gold and bitcoin investment products. Then, according to the time range of gold and bitcoin assets, three types of transactions were determined: cross, non-cross, and inclusion relationship, and the daily trading strategies were determined by the greedy model established by a greedy algorithm. Then, the Sharpe Ratio of the nonparametric method was used to measure the risk of the developed decision model and evaluate the accuracy of the model. Finally, starting from the stock market fluctuation and macro-mobilization, the sensitivity of the decision model under different transaction costs was tested by increasing or decreasing the percentage of transaction costs (0.5%, 1%, 1.5%, and 2%, respectively). Research informs investors on how to invest for the best returns.

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that hosts complex signaling events and plays diverse roles in a variety of cellular processes. Secreted ER chaperones are capable of activating EGFR and its downstream transcription factor, STAT3. However, the molecular mechanism of these ER proteins triggering EGFR signaling is largely unknown. In this study, we identify KDEL receptor (KDELR) as a cell surface receptor for secreted ER chaperones and a co-receptor for EGFR. Prior to ligand addition, KDELR spontaneously oligomerizes and constantly undergoes recycling near the plasma membrane. Upon ligand binding, the interactions of KDELR with itself and with EGFR increase rapidly, leading to augmented internalization of KDELR and tyrosine phosphorylation in the C-terminus of EGFR. STAT3, which binds the phosphorylated tyrosine motif on EGFR, is subsequently activated by EGFR and induces transcription of genes relevant to cell proliferation and migration. Taken together, our results suggest that secreted ER chaperones bind surface-expressed KDELR and transactivates EGFR-STAT3 signaling pathway.

KDEL receptor-1 maintains homeostasis in the early secretory pathway by capturing and retrieving ER-chaperones to the ER during heavy secretory activity. We have previously shown that a Golgi scaffolding protein (ACBD3) facilitates KDEL receptor localization at the Golgi via regulating cargo wave-induced cAMP/PKA-dependent signaling pathway. Unexpectedly, a fraction of the receptor is also known to reside in the plasma membrane as a stress response, although it is largely unknown exactly how KDEL receptor gets exported from the Golgi and travels to the PM. In this study, we sought to investigate the mechanism by which KDEL receptor gets exported from the Golgi en route to the PM and identified two crucial factors that greatly influence post-Golgi trafficking of KDEL receptor. We show here that ACBD3 depletion results in significantly increased trafficking of KDEL receptor to the PM via Rab4-positive tubular carriers emanating from the Golgi. Expression of constitutively activated Rab4 mutant (Q72L) increases surface expression of KDEL receptor up to 2~3-fold, whereas expression of GDP-locked Rab4 mutant (S27N) inhibits KDEL receptor localization to the PM. Importantly, KDELR trafficking from the Golgi to the PM is independent of PKA- and Src Kinase-mediated mechanism. Taken together, these results reveal that ACBD3 and Rab4-GTP are key players at the Golgi in regulating KDEL receptor trafficking to the cell surface.

SUMOylation is a post-translational modification that attaches a ubiquitin-like modifier to target proteins and plays a number of roles throughout the cells, including transcriptional regulation and cellular stress response, etc. We have recently reported that a Golgi structural protein, Golgin45, is subjected to Tankyrase1-dependent poly (ADP-Ribosyl)ation, resulting in dynamic regulation of Golgin45 protein and membrane trafficking of glycosylating enzymes at the Golgi. However, exact mechanism that modulates Tankyrase activity on Golgin45 remained elusive. Here, we report that Golgin45 is a SUMOylated protein, and heat shock significantly increases Golgin45 SUMOylation, concomitantly leading to reduced PARylation. Importantly, while PML is required for Golgin45 SUMOylation by SUMO3, over-expression of a SUMOylation-deficient Golgin45 mutant inhibits the formation of PML-NBs under heat shock, revealing a role of SUMOylated Golgin45 in PML-NB formation in the nucleus. These results provide a new insight into stress-induced inter-organelle communications between the Golgi and the nucleus.

A rhodamine-based fluorescent probe for Cu2+ and ATP has been designed. The fluorescence intensity/absorbance was significantly enhanced upon the addition of Cu2+ owning to the opening of the spiro-ring of rhodamine, which quickly returned to the original level due to the reconstruction of the probe by the reacting with ATP. Cu2+/ATP-induced fluorescent intensity/aborbance changes showed a good linear relationship with the concentration of Cu2+/ATP in the range of 2–20 μM/0–10 μM with a detection limit of 0.1 μM/1.0 μM. The proposed method is simple in design and fast in operation, and is suitable for the reversible monitoring of Cu2+ and ATP in bioanalytical applications.

In the context of high loss in the storage and transportation of fresh agricultural products, in order to help company make reasonable fresh-keeping decisions and reduce losses, we established a leading supplier of fresh agricultural products in two level dual channel supply chain model based on consumer utility function, and using Stackelberg game method to solve the optimal pricing and optimal fresh-keeping decision of fresh agricultural supplier and retailer under centralized decision-making and decentralized decision-making model. Research shows: (1) Under centralized decision-making model, the highest profit does not affect the cooperation and achieve complete coordination regardless of the bargaining power of the retailer; (2) High cost factor of fresh-keeping efforts makes supplier and retailer more inclined to lower prices to attract consumers. (3)The “revenue sharing + fresh-keeping cost sharing” coordination strategy provided by the supplier can increase the respective profits of both parties and achieve complete coordination of the dual-channel supply chain of fresh agricultural products.

Liposomes are of great interest and importance in tumor imaging, since they can greatly improve the imaging sensitivity and specificity by increasing the accumulation of contrast agents. Still, most liposome-based probes have high background signals during blood circulation, which limits enhancement of S/B ratio and tumor imaging sensitivity. To enhance the S/B ratio of tumor imaging, we construct a fluorescence resonance energy transfer (FRET) and aggregation induced emission (AIE) based liposomal fluorescence probe TPE/BHQ-lipo with excellent FRET effect (99 %) and great fluorescence enhancement upon liposome rupture (120-fold) as well as efficient fluorescence recovery in tumor cell imaging. Finally, we used the TPE/BHQ-lipo to image 4T1 tumor upon intravenous injection of liposomes and the group showed enhanced signal to background ratio of 4.1, compared to 1.8 from control AIE-based liposomal group (TPE-lipo). Our work offers an excellent FRET and AIE-based liposomal probe for high-sensitive tumor imaging.

Herein, we presented two novel turn-on colorimetric and fluorescent probes based on a F− triggered Si O bond cleavage reaction, which displayed several desired properties for the quantitative detection for F−, such as high specificity, rapid response time (within 3 min) and naked-eye visualization. The fluorescence intensity at 574 nm (absorbance at 544 nm) of the solution was found to increase linearly with the concentration of F− (0.00–30.0 μM) with the detection limit was estimated to be 0.47 μM/0.48 μM. Based on these excellent optical properties, the probes were employed to monitor F− in real water samples and tea samples with satisfactory. Furthermore, it was successfully applied for fluorescent imaging of F− in living nude mice, suggesting that it could be used as a powerful tool to predict and explore the biological functions of F− in physiological and pathological processes.

A rhodamine-based fluorescent probe for Cu